The Experts below are selected from a list of 31743 Experts worldwide ranked by ideXlab platform
Angélique Vetillard - One of the best experts on this subject based on the ideXlab platform.
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De Novo sequencing and transcriptome analysis for Tetramorium bicarinatum: a comprehensive venom gland transcriptome analysis from an ant species
BMC Genomics, 2014Co-Authors: Wafa Bouzid, Marion Verdenaud, Christophe Klopp, Frédéric Ducancel, Celine Noirot, Angélique VetillardAbstract:Background: Arthropod venoms are invaluable sources of bioactive substances with Biotechnological Application. The limited availability of some venoms, such as those from ants, has restricted the knowledge about the composition and the potential that these biomolecules could represent. In order to provide a global insight on the transcripts expressed in the venom gland of the Brazilian ant species Tetramorium bicarinatum and to unveil the potential of its products, high-throughput approach using Illumina technology has been applied to analyze the genes expressed in active venom glands of this ant species.
Subhankar Chatterjee - One of the best experts on this subject based on the ideXlab platform.
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Psychrotolerant Sphingobacterium kitahiroshimense LT-2 Isolated from Dhundi Glacier, Himachal Pradesh: Origin Prediction and Future Application.
Indian Journal of Microbiology, 2018Co-Authors: Shivika Sharma, Subhankar ChatterjeeAbstract:A psychrotolerant bacterium, isolated from Dhundi Glacier, Himachal Pradesh (India) was identified as Sphingobacterium kitahiroshimense LT-2 on the basis of biochemical, molecular and phylogenetic analysis. Sphingobacterium kitahiroshimense was first reported from Japan and was isolated from the city of Kitahiroshima, Hokkaido, Japan. In this report we have discussed about the origin of our strain and predicted that air masses and dust associated microbial cells transportation phenomena may be applicable for the origin of this species in this region. Enzymes and secondary metabolites secreted by the genus Sphingobacterium have enormous potentiality regarding their Biotechnological Application. Preliminary study of our strain based on metabolic profiling through HPLC showed many new metabolites were secreted by the bacterium when grown in presence of different sugar medium at 28 °C. As far as our knowledge this is the first report about Sphingobacterium species isolated from this region. This preliminary finding will help to draw an idea about the bacterial population in this Himalayan Glaciers (in HP) as well as Biotechnological Application of this strain can be explored further.
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Psychrotolerant Sphingobacterium kitahiroshimense LT-2 Isolated from Dhundi Glacier, Himachal Pradesh: Origin Prediction and Future Application.
Indian Journal of Microbiology, 2018Co-Authors: Shivika Sharma, Subhankar ChatterjeeAbstract:A psychrotolerant bacterium, isolated from Dhundi Glacier, Himachal Pradesh (India) was identified as Sphingobacterium kitahiroshimense LT-2 on the basis of biochemical, molecular and phylogenetic analysis. Sphingobacterium kitahiroshimense was first reported from Japan and was isolated from the city of Kitahiroshima, Hokkaido, Japan. In this report we have discussed about the origin of our strain and predicted that air masses and dust associated microbial cells transportation phenomena may be applicable for the origin of this species in this region. Enzymes and secondary metabolites secreted by the genus Sphingobacterium have enormous potentiality regarding their Biotechnological Application. Preliminary study of our strain based on metabolic profiling through HPLC showed many new metabolites were secreted by the bacterium when grown in presence of different sugar medium at 28 °C. As far as our knowledge this is the first report about Sphingobacterium species isolated from this region. This preliminary finding will help to draw an idea about the bacterial population in this Himalayan Glaciers (in HP) as well as Biotechnological Application of this strain can be explored further.
Wafa Bouzid - One of the best experts on this subject based on the ideXlab platform.
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De Novo sequencing and transcriptome analysis for Tetramorium bicarinatum: a comprehensive venom gland transcriptome analysis from an ant species
BMC Genomics, 2014Co-Authors: Wafa Bouzid, Marion Verdenaud, Christophe Klopp, Frédéric Ducancel, Celine Noirot, Angélique VetillardAbstract:Background: Arthropod venoms are invaluable sources of bioactive substances with Biotechnological Application. The limited availability of some venoms, such as those from ants, has restricted the knowledge about the composition and the potential that these biomolecules could represent. In order to provide a global insight on the transcripts expressed in the venom gland of the Brazilian ant species Tetramorium bicarinatum and to unveil the potential of its products, high-throughput approach using Illumina technology has been applied to analyze the genes expressed in active venom glands of this ant species.
Rita Bernhardt - One of the best experts on this subject based on the ideXlab platform.
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Cytochromes P450 as promising catalysts for Biotechnological Application: chances and limitations
Applied Microbiology and Biotechnology, 2014Co-Authors: Rita Bernhardt, Vlada B. UrlacherAbstract:Cytochromes P450 (CYPs) belong to the superfamily of heme b containing monooxygenases with currently more than 21,000 members. These enzymes accept a vast range of organic molecules and catalyze diverse reactions. These extraordinary capabilities of CYP systems that are unmet by other enzymes make them attractive for biotechnology. However, the complexity of these systems due to the need of electron transfer from nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) via redox partner proteins for the initial hydroxylation step limits a broader technical implementation of CYP enzymes. There have been several reviews during the past years tackling the potential CYPs for synthetic Application. The aim of this review is to give a critical overview about possibilities and chances for Application of these interesting catalysts as well as to discuss drawbacks and problems related to their use. Solutions to overcome these limitations will be demonstrated, and several selected examples of successful CYP Applications under industrial conditions will be reviewed.
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Investigation of cytochromes P450 in myxobacteria: Excavation of cytochromes P450 from the genome of Sorangium cellulosum So ce56
FEBS Letters, 2011Co-Authors: Yogan Khatri, Olena Perlova, Frank Hannemann, Rolf Müller, Rita BernhardtAbstract:The exploitation of cytochromes P450 for novel Biotechnological Application and for the investigation of their physiological function is of great scientific interest in this post genomic era, where an extraordinary biodiversity of P450 genes has been derived from all forms of life. The study of P450s in the myxobacterium Sorangium cellulosum strain So ce56, the producer of novel secondary metabolites of pharmaceutical interest is the research topic, in which we were engaged since the beginning of its genome sequencing project. We herein disclosed the cytochrome P450 complements (CYPomes) of spore-forming myxobacterial species, Stigmatella aurantiaca DW4/3-1, Haliangium ochraceum DSM 14365 and Myxococcus xanthus DK1622, and their potential pharmaceutical significance has been discussed.
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discovery of a steroid 11α hydroxylase from rhizopus oryzae and its Biotechnological Application
Journal of Biotechnology, 2010Co-Authors: S Petric, Rita Bernhardt, Tarek Hakki, D žigon, B CresnarAbstract:Abstract To overcome the chemically laborious stereo- and regioselective hydroxylation steps in the pharmaceutical production of corticosteroids and progestogens, certain fungal species, e.g. Rhizopus spp. and Aspergillus spp., are employed to perform the 11α-hydroxylation of the steroid skeleton, thereby significantly simplifying steroid drug production. Here we report for the first time the identification and expression of a fungal 11α-steroid hydroxylase, CYP509C12. The newly identified cytochrome P450, which is one of the 48 putative CYP genes in Rhizopus oryzae , was induced in the fungus by progesterone. By functionally expressing CYP509C12 in recombinant fission yeast, we were able to determine that its substrate spectrum includes progesterone as well as testosterone, 11-deoxycorticosterone, and 11-deoxycortisol, with the hydroxylations taking place predominantly at 11α and 6β positions of the steroid ring system. To increase the 11α-hydroxylation activity of CYP509C12 in recombinant fission yeast, its natural redox partner, the R. oryzae NAD(P)H-dependent reductase, was coexpressed. The coexpression improved electron transfer to CYP509C12 and thus an increase in productivity from 246 to 300 μM hydroxyPg d −1 was observed, as well as a 7-fold increase of rate of hydroxyprogesterone formation within the linear phase of transformation. This newly developed strain displayed total bioconversion of progesterone into 11α-hydroxyprogesterone and small amounts of 6β-hydroxyprogesterone within the first 6 h of incubation with progesterone as substrate, hence demonstrating its potential for Biotechnological Application.
Shivika Sharma - One of the best experts on this subject based on the ideXlab platform.
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Psychrotolerant Sphingobacterium kitahiroshimense LT-2 Isolated from Dhundi Glacier, Himachal Pradesh: Origin Prediction and Future Application.
Indian Journal of Microbiology, 2018Co-Authors: Shivika Sharma, Subhankar ChatterjeeAbstract:A psychrotolerant bacterium, isolated from Dhundi Glacier, Himachal Pradesh (India) was identified as Sphingobacterium kitahiroshimense LT-2 on the basis of biochemical, molecular and phylogenetic analysis. Sphingobacterium kitahiroshimense was first reported from Japan and was isolated from the city of Kitahiroshima, Hokkaido, Japan. In this report we have discussed about the origin of our strain and predicted that air masses and dust associated microbial cells transportation phenomena may be applicable for the origin of this species in this region. Enzymes and secondary metabolites secreted by the genus Sphingobacterium have enormous potentiality regarding their Biotechnological Application. Preliminary study of our strain based on metabolic profiling through HPLC showed many new metabolites were secreted by the bacterium when grown in presence of different sugar medium at 28 °C. As far as our knowledge this is the first report about Sphingobacterium species isolated from this region. This preliminary finding will help to draw an idea about the bacterial population in this Himalayan Glaciers (in HP) as well as Biotechnological Application of this strain can be explored further.
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Psychrotolerant Sphingobacterium kitahiroshimense LT-2 Isolated from Dhundi Glacier, Himachal Pradesh: Origin Prediction and Future Application.
Indian Journal of Microbiology, 2018Co-Authors: Shivika Sharma, Subhankar ChatterjeeAbstract:A psychrotolerant bacterium, isolated from Dhundi Glacier, Himachal Pradesh (India) was identified as Sphingobacterium kitahiroshimense LT-2 on the basis of biochemical, molecular and phylogenetic analysis. Sphingobacterium kitahiroshimense was first reported from Japan and was isolated from the city of Kitahiroshima, Hokkaido, Japan. In this report we have discussed about the origin of our strain and predicted that air masses and dust associated microbial cells transportation phenomena may be applicable for the origin of this species in this region. Enzymes and secondary metabolites secreted by the genus Sphingobacterium have enormous potentiality regarding their Biotechnological Application. Preliminary study of our strain based on metabolic profiling through HPLC showed many new metabolites were secreted by the bacterium when grown in presence of different sugar medium at 28 °C. As far as our knowledge this is the first report about Sphingobacterium species isolated from this region. This preliminary finding will help to draw an idea about the bacterial population in this Himalayan Glaciers (in HP) as well as Biotechnological Application of this strain can be explored further.