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Toru Nagasawa - One of the best experts on this subject based on the ideXlab platform.
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occurrence of e poly l lysine degrading enzyme in e poly l lysine tolerant Sphingobacterium multivorum oj10 purification and characterization
Fems Microbiology Letters, 2002Co-Authors: Mitsuaki Kito, Yuichi Onji, Toyokazu Yoshida, Toru NagasawaAbstract:Abstract ϵ-Poly- L -lysine (ϵ-PL)-degrading enzyme was found in the ϵ-PL-tolerant strain Sphingobacterium multivorum OJ10 and purified to homogeneity. The purified enzyme has a molecular mass of approximately 80 kDa. The enzyme catalyzed exo-type degradation of ϵ-PL and released L -lysine. The enzyme was a Co 2+ or Ca 2+ ion-activated aminopeptidase.
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occurrence of epsilon poly l lysine degrading enzyme in epsilon poly l lysine tolerant Sphingobacterium multivorum oj10 purification and characterization
Fems Microbiology Letters, 2002Co-Authors: Mitsuaki Kito, Yuichi Onji, Toyokazu Yoshida, Toru NagasawaAbstract:?-Poly-l-lysine (?-PL)-degrading enzyme was found in the ?-PL-tolerant strain Sphingobacterium multivorum OJ10 and purified to homogeneity. The purified enzyme has a molecular mass of approximately 80 kDa. The enzyme catalyzed exo-type degradation of ?-PL and released l-lysine. The enzyme was a Co2+ or Ca2+ ion-activated aminopeptidase.
Akira Yokota - One of the best experts on this subject based on the ideXlab platform.
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Sphingobacterium thermophilum sp nov of the phylum bacteroidetes isolated from compost
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Shuhei Yabe, Kazuyoshi Kawahara, Yoshifumi Aiba, Yasuteru Sakai, Masaru Hazaka, Akira YokotaAbstract:A Gram-negative bacterium, designated CKTN2T, was isolated from compost. Cells of strain CKTN2T were strictly aerobic rods. The isolate grew at 20–50 °C (optimum 40–45 °C), but not below 15 °C or above 52 °C, and at pH 5.9–8.8 (optimum pH 7.0), but not below pH 5.4 or above pH 9.3. The DNA G+C content was 40.3 mol%. The predominant menaquinone was MK-7. The major fatty acids were iso-C15 : 0 (45.2 %), iso-C17 : 0 3-OH (11.1 %) and C18 : 0 (14.5 %). Analysis of the 16S rRNA gene sequence of strain CKTN2T revealed that it is a member of the genus Sphingobacterium and is most closely related to Sphingobacterium alimentarium DSM 22362T (93.2 % 16S rRNA gene sequence similarity). Strain CKTN2T could be distinguished from its closest phylogenetic relatives by different phenotypic characteristics. According to the phenotypic and genotypic characteristics, strain CKTN2T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium thermophilum sp. nov. is proposed. The type strain is CKTN2T ( = JCM 17858T = KCTC 23708T).
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proposals of Sphingobacterium faecium sp nov Sphingobacterium piscium sp nov Sphingobacterium heparinum comb nov Sphingobacterium thalpophilum comb nov and two genospecies of the genus Sphingobacterium and synonymy of flavobacterium yabuuchiae and sp
Journal of General and Applied Microbiology, 1992Co-Authors: Mariko Takeuchi, Akira YokotaAbstract:Seventeen strains of Cytophaga, Flavobacterium and Actinobacillus species were taxonomically characterized. DNA base composition of these strains ranged from 39.0 to 42.3mol%, and the menaquinone system was MK-7. All the strains contained sphingolipids with long-chain bases, which are characteristic of the genus Sphingobacterium. Based on their chemotaxonomic and physiological characteristics together with DNA/DNA hybridization studies, we propose two new species and two new combinations, Sphingobacterium faecium sp. nov., Sphingobacterium piscium sp. nov., Sphingobacterium heparinum comb. nov., Sphingobacterium thalpophilum comb. nov. and two genospecies of the genus Sphingobacterium. Flavobacterium yabuuchiae was found to be a synonym of Sphingobacterium spiritivorum.
Erkin Rahman - One of the best experts on this subject based on the ideXlab platform.
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Sphingobacterium hotanense sp nov isolated from soil of a populus euphratica forest and emended descriptions of Sphingobacterium daejeonense and Sphingobacterium shayense
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Ting Xiao, Xiang He, Gang Cheng, Haiju Kuang, Khayir Yusup, Maryam Hamdun, Abaidulla Gulsimay, Chengxiang Fang, Erkin RahmanAbstract:A novel Gram-staining-negative bacterial strain, designated XH4T, was isolated from soil of a Populus euphratica forest in the Hotan River valley, Xinjiang Uyghur autonomous region, PR China. The cells were strictly aerobic, non-motile, short rods. The isolate grew optimally at 37 °C and at pH 7.0–8.0. Based on 16S rRNA gene sequence analysis, strain XH4T belonged to the genus Sphingobacterium and was closely related to Sphingobacterium mizutaii ATCC 33299T (96.1 % sequence similarity). The DNA G+C content was 41.2 mol%. The major polar lipid of strain XH4T was phosphatidylethanolamine, and several unidentified polar lipids were also present. Strain XH4T showed the typical chemotaxonomic features of the genus Sphingobacterium , with the presence of ceramide phosphorylethanolamine 2 (CerPE-2) as the major ceramide. The major cellular fatty acids of strain XH4T were iso-C15 : 0 (34.0 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 20.7 %) and iso-C17 : 0 3-OH (14.7 %). The predominant isoprenoid quinone was MK-7. On the basis of phenotypic data and phylogenetic inference, strain XH4T represents a novel species of the genus Sphingobacterium , for which the name Sphingobacterium hotanense sp. nov. is proposed. The type strain is XH4T ( = NRRL B-59204T = CCTCC AB 209007T). Emended descriptions of Sphingobacterium daejeonense and Sphingobacterium shayense are also given.
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Sphingobacterium shayense sp nov isolated from forest soil
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Xiang He, Ting Xiao, Haiju Kuang, Chengxiang Fang, Maripat Tudahong, Ghenijan Osman, Erkin RahmanAbstract:A Gram-staining-negative, yellow-coloured, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated HS39T, isolated from a soil sample collected from a natural Populus euphratica forest in Xinjiang, China, was characterized using a polyphasic approach. The isolate grew optimally at 30–37 °C, at pH 6.5–8.0 and with 0–3 % NaCl. Analysis of the 16S rRNA gene sequence of strain HS39T revealed that it is a member of the genus Sphingobacterium. Sphingobacterium mizutaii ATCC 33299T was the nearest relative (94.0 % 16S rRNA gene sequence similarity). The G+C content of the genomic DNA was 40.2 mol%. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c). The predominant isoprenoid quinone was MK-7. On the basis of phenotypic properties and phylogenetic inference, strain HS39T represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium shayense sp. nov. is proposed. The type strain is HS39T (=CCTCC AB 209006T =NRRL B-59203T).
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Sphingobacterium shayense sp. nov., isolated from forest soil.
International journal of systematic and evolutionary microbiology, 2009Co-Authors: Xiang He, Ting Xiao, Haiju Kuang, Chengxiang Fang, Maripat Tudahong, Ghenijan Osman, Erkin RahmanAbstract:A Gram-staining-negative, yellow-coloured, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated HS39(T), isolated from a soil sample collected from a natural Populus euphratica forest in Xinjiang, China, was characterized using a polyphasic approach. The isolate grew optimally at 30-37 °C, at pH 6.5-8.0 and with 0-3 % NaCl. Analysis of the 16S rRNA gene sequence of strain HS39(T) revealed that it is a member of the genus Sphingobacterium. Sphingobacterium mizutaii ATCC 33299(T) was the nearest relative (94.0 % 16S rRNA gene sequence similarity). The G+C content of the genomic DNA was 40.2 mol%. The major fatty acids were iso-C₁₅:₀, iso-C₁₇:₀ 3-OH and summed feature 3 (comprising C₁₆:₁ω6c and/or C₁₆:₁ω7c). The predominant isoprenoid quinone was MK-7. On the basis of phenotypic properties and phylogenetic inference, strain HS39(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium shayense sp. nov. is proposed. The type strain is HS39(T) (=CCTCC AB 209006(T) =NRRL B-59203(T)).
Hans-jürgen Busse - One of the best experts on this subject based on the ideXlab platform.
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Sphingobacterium cellulitidis sp. nov., isolated from clinical and environmental sources.
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Geert Huys, Prashant Purohit, Cindy Snauwaert, Huda Al Saffar, Ina'am Al Obaid, Hans-jürgen Busse, Torsten Seemann, M. John AlbertAbstract:The taxonomic position of two isolates belonging to the genus Sphingobacterium was determined. The first isolate, R-53603T, was obtained from purulent discharge from the toe of a cellulitis patient in Kuwait. Comparative 16S rRNA gene sequence analysis revealed 99.87 % similarity of R-53603T with environmental isolate P031 (=R-53745) originating from activated sludge in Singapore. The two isolates were phylogenetically positioned on the same sub-branch. Highest 16S rRNA gene sequence similarity was found with the type strains of Sphingobacterium mizutaii (98.23 %), Sphingobacterium lactis (97.78 %) and Sphingobacterium daejeonense (97.14 %). DNA–DNA hybridizations revealed
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Sphingobacterium cellulitidis sp nov isolated from clinical and environmental sources
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Geert Huys, Prashant Purohit, Cindy Snauwaert, Huda Al Saffar, Ina'am Al Obaid, Hans-jürgen Busse, Torsten Seemann, John M AlbertAbstract:The taxonomic position of two isolates belonging to the genus Sphingobacterium was determined. The first isolate, R-53603T, was obtained from purulent discharge from the toe of a cellulitis patient in Kuwait. Comparative 16S rRNA gene sequence analysis revealed 99.87 % similarity of R-53603T with environmental isolate P031 (=R-53745) originating from activated sludge in Singapore. The two isolates were phylogenetically positioned on the same sub-branch. Highest 16S rRNA gene sequence similarity was found with the type strains of Sphingobacterium mizutaii (98.23 %), Sphingobacterium lactis (97.78 %) and Sphingobacterium daejeonense (97.14 %). DNA–DNA hybridizations revealed <70 % relatedness between the two isolates and the type strains of the close phylogenetic neighbours S. mizutaii (18.0–24.5 %), S. lactis (20.3–25.9 %) and S. daejeonense (13.2–20.0 %). The high relative contribution of iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c) in the cellular fatty acid profiles of R-53603T and R-53745, the presence of sphingophospholipids, MK-7 as the dominant menaquinone and phosphatidylethanolamine as the major polar lipid in strain R-53603T are typical chemotaxonomic characteristics for members of the genus Sphingobacterium . Phenotypic features most useful for differentiation of the two novel strains from the most closely related species S. mizutaii include growth on MacConkey agar, and utilization of stachyose, guanidine HCl and lithium chloride in Biolog GEN III tests. Strains R-53603T and R-53745 thus represent a novel species, for which the name Sphingobacterium cellulitidis sp. nov. is proposed. The type strain is R-53603T (=LMG 28764T=DSM 102028T).
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Sphingobacterium zeae sp nov an endophyte of maize
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Peter Kampfer, Hans-jürgen Busse, Tanita Kleinhagauer, John A Mcinroy, Stefanie P GlaeserAbstract:A yellow-pigmented strain (JM-1081T) isolated from healthy stem tissue of Zea mays was taxonomically characterized. Cells of the strain were rod-shaped and Gram-stain-negative. Comparative 16S rRNA gene sequence analysis revealed closest relationship to the type strains of Sphingobacterium multivorum (98.1 % similarity), Sphingobacterium mucilaginosum (97.9 %) and Sphingobacterium siyangense (97.8 %). 16S rRNA gene sequence similarities to the type strains of all other Sphingobacterium species were below 97.8 %. Fatty acid analysis of whole-cell hydrolysates of the strain resulted in a pattern typical of the genus Sphingobacterium with iso-C15 : 0 2-OH and/or C16 : 1 ω7c, iso-C15 : 0, iso-C17 : 0 3-OH and C16 : 0 and as major compounds. The polyamine pattern contained predominantly sym-homospermidine. The major quinone was menaquinone MK-7 and the only identified lipids in the polar lipid profile were phosphatidylethanolamine and phosphatidylserine. In addition, 15 unidentified lipids were detected in moderate to major amounts. Sphingolipid was detected. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. DNA–DNA hybridizations with two of the closely related type strains, those of S. multivorum and S. siyangense , as well as Sphingobacterium canadense resulted in values below 70 %. In addition to the genotypic differences, differential biochemical and chemotaxonomic properties confirmed that the isolate JM-1081T represents a novel species, for which the name Sphingobacterium zeae sp. nov. is proposed. The type strain is JM-1081T (=LMG 29191T=CCM 8652T).
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Sphingobacterium psychroaquaticum sp nov a psychrophilic bacterium isolated from lake michigan water
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Richard A Albert, Nancy E Waas, Shawn C Pavlons, Jamie L Pearson, Laura M Ketelboeter, Ramon Rossellomora, Hans-jürgen BusseAbstract:A psychrophilic, Gram-negative bacterium, designated MOL-1T, was isolated from water of Lake Michigan. 16S rRNA gene sequence analysis revealed that the sequence of strain MOL-1T has sequence similarity of 95.6, 94.8, 94.3, 94.3, 94.2 and 93.9 %, respectively, to the 16S rRNA gene sequences of Sphingobacterium shayense HS39T, S. lactis WCC 4512T, S. composti T5-12T, S. daejeonense TR6-04T, S. bambusae IBFC2009T and S. alimentarium WCC 4521T. The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). Menaquinone MK-7 is the predominant respiratory quinone, while sym-homospermidine is the predominant polyamine. The polar lipid profile is composed of the predominant lipids phosphatidylethanolamine and unidentified polar lipid L2, with moderate amounts of unidentified polar lipids L1, L5 and L6 and unidentified aminophospholipids APL1 and APL2 and minor to trace amounts of unidentified polar lipids L3, L4, L7, L8, L9 and L10, unidentified phospholipid PL4 and unidentified aminophospholipid APL3. After molecular and phenotypic studies, including chemotaxonomic analyses, it was concluded that strain MOL-1T represents a novel Sphingobacterium species, for which the name Sphingobacterium psychroaquaticum sp. nov. is proposed. The type strain is MOL-1T ( = NRRL B-59232T = DSM 22418T).
Mitsuaki Kito - One of the best experts on this subject based on the ideXlab platform.
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occurrence of e poly l lysine degrading enzyme in e poly l lysine tolerant Sphingobacterium multivorum oj10 purification and characterization
Fems Microbiology Letters, 2002Co-Authors: Mitsuaki Kito, Yuichi Onji, Toyokazu Yoshida, Toru NagasawaAbstract:Abstract ϵ-Poly- L -lysine (ϵ-PL)-degrading enzyme was found in the ϵ-PL-tolerant strain Sphingobacterium multivorum OJ10 and purified to homogeneity. The purified enzyme has a molecular mass of approximately 80 kDa. The enzyme catalyzed exo-type degradation of ϵ-PL and released L -lysine. The enzyme was a Co 2+ or Ca 2+ ion-activated aminopeptidase.
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occurrence of epsilon poly l lysine degrading enzyme in epsilon poly l lysine tolerant Sphingobacterium multivorum oj10 purification and characterization
Fems Microbiology Letters, 2002Co-Authors: Mitsuaki Kito, Yuichi Onji, Toyokazu Yoshida, Toru NagasawaAbstract:?-Poly-l-lysine (?-PL)-degrading enzyme was found in the ?-PL-tolerant strain Sphingobacterium multivorum OJ10 and purified to homogeneity. The purified enzyme has a molecular mass of approximately 80 kDa. The enzyme catalyzed exo-type degradation of ?-PL and released l-lysine. The enzyme was a Co2+ or Ca2+ ion-activated aminopeptidase.
