The Experts below are selected from a list of 16362 Experts worldwide ranked by ideXlab platform
Peter Dürre - One of the best experts on this subject based on the ideXlab platform.
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fermentative butanol production
Annals of the New York Academy of Sciences, 2008Co-Authors: Peter DürreAbstract:Clostridium acetobutylicum is an anaerobic, spore-forming bacterium with the ability to ferment starch and sugars into solvents. In the past, it has been used for industrial production of acetone and butanol, until cheap crude oil rendered petrochemical synthesis more economically feasible. Both economic (price of crude oil) and environmental aspects (carbon dioxide emissions) have caused the pendulum to swing back again. Molecular biology has allowed a detailed understanding of genes and enzymes, required for solventogenesis. Thus, construction of strains with improved fermentation ability is now possible. Advances in continuous culture technology and improved downstream Processing also add to economic advantages of a new Biotechnological Process. Two major companies have already committed themselves to biobutanol production as a biofuel additive. Thus, butanol fermentation is on the rise again.
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Fermentative butanol production: Bulk chemical and biofuel
Annals of the New York Academy of Sciences, 2008Co-Authors: Peter DürreAbstract:Clostridium acetobutylicum is an anaerobic, spore-forming bacterium with the ability to ferment starch and sugars into solvents. In the past, it has been used for industrial production of acetone and butanol, until cheap crude oil rendered petrochemical synthesis more economically feasible. Both economic (price of crude oil) and environmental aspects (carbon dioxide emissions) have caused the pendulum to swing back again. Molecular biology has allowed a detailed understanding of genes and enzymes, required for solventogenesis. Thus, construction of strains with improved fermentation ability is now possible. Advances in continuous culture technology and improved downstream Processing also add to economic advantages of a new Biotechnological Process. Two major companies have already committed themselves to biobutanol production as a biofuel additive. Thus, butanol fermentation is on the rise again.
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biobutanol an attractive biofuel
Biotechnology Journal, 2007Co-Authors: Peter DürreAbstract:Biofuels are an attractive means to prevent a further increase of carbon dioxide emissions. Currently, gasoline is blended with ethanol at various percentages. However, butanol has several advantages over ethanol, such as higher energy content, lower water absorption, better blending ability, and use in conventional combustion engines without modification. Like ethanol, it can be produced fermentatively or petrochemically. Current crude oil prices render the Biotechnological Process economic again. The best-studied bacterium to perform a butanol fermentation is Clostridium acetobutylicum. Its genome has been sequenced, and the regulation of solvent formation is under intensive investigation. This opens the possibility to engineer recombinant strains with superior biobutanol-producing ability.
Sueli Rodrigues - One of the best experts on this subject based on the ideXlab platform.
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Dextransucrase Stability in Cashew Apple Juice
Food and Bioprocess Technology, 2008Co-Authors: Talita L. Honorato, Sueli RodriguesAbstract:The use of agriculture waste as substrate for Biotechnological Process became an interesting alternative to reduce production costs and the negative impact of agricultural wastes disposal in the environment. In Brazil, cashew tree cultivation is done aiming the cashew nut production, and tons of cashew apples are wasted in the field after the nut removal. Thus, cashew apple juice can be used as low cost substrate for Biotechnological Process. In this study, the stability of dextransucrase produced in a fermentation medium using cashew apple juice as substrate was evaluated. The crude enzyme was stable at 30 °C for 30 h at pH ranging from 4.5 to 5.5. The partially purified enzyme was stable in non-fermented cashew apple juice at pH 5.0 for 96 h at 30 °C. The high stability of the enzyme in cashew apple juice at room temperature allows its industrial use without needing the enzyme purification, consequently reducing Process costs.
Julio Cesar Dos Santos - One of the best experts on this subject based on the ideXlab platform.
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biosurfactant production by aureobasidium pullulans in stirred tank bioreactor new approach to understand the influence of important variables in the Process
Bioresource Technology, 2017Co-Authors: Larissa Pereira Brumano, Julio Cesar Dos Santos, Felipe A F Antunes, Sara Galeno Souto, Joachim Venus, Roland Schneider, Silvio Silverio Da SilvaAbstract:Abstract Surfactants are amphiphilic molecules with large industrial applications produced currently by chemical routes mainly derived from oil industry. However, Biotechnological Process, aimed to develop new sustainable Process configurations by using favorable microorganisms, already requires investigations in more details. Thus, we present a novel approach for biosurfactant production using the promising yeast Aureobasidium pullulans LB 83, in stirred tank reactor. A central composite face-centered design was carried out to evaluate the effect of the aeration rate (0.1–1.1 min −1 ) and sucrose concentration (20–80 g.L −1 ) in the biosurfactant maximum tensoactivity and productivity. Statistical analysis showed that the use of variables at high levels enhanced tensoactivity, showing 8.05 cm in the oil spread test and productivity of 0.0838 cm.h −1 . Also, unprecedented investigation of aeration rate and sucrose concentration relevance in biosurfactant production by A. pullulans in stirred tank reactor was detailed, demonstrating the importance to establish adequate conditions in bioreactors, aimed to scale-up Process.
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low melanin containing pullulan production from sugarcane bagasse hydrolysate by aureobasidium pullulans in fermentations assisted by light emitting diode
Bioresource Technology, 2017Co-Authors: Ruly Teran Hilares, Camila Ayres Orsi, Muhammad Ajaz Ahmed, Paulo Ricardo Franco Marcelino, Carlos Renato Menegatti, Silvio Silverio Da Silva, Julio Cesar Dos SantosAbstract:Abstract Pullulan is a polymer produced by Aureobasidium pullulans and the main bottleneck for its industrial production is the presence of melanin pigment. In this study, light-emitting diodes (LEDs) of different wavelengths were used to assist the fermentation Process aiming to produce low-melanin containing pullulan by wild strain of A. pullulans LB83 with different carbon sources. Under white light using glucose-based medium, 11.75 g.L −1 of pullulan with high melanin content (45.70 UA 540nm .g −1 ) was obtained, this production improved in Process assisted by blue LED light, that resulted in 15.77 g.L −1 of pullulan with reduced content of melanin (4.46 UA 540nm .g −1 ). By using sugarcane bagasse (SCB) hydrolysate as carbon source, similar concentration of pullulan (about 20 g.L −1 ) was achieved using white and blue LED lights, with lower melanin contents in last. Use of LED light was found as a promising approach to assist Biotechnological Process for low-melanin containing pullulan production.
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scale up of xylitol production from sugarcane bagasse hemicellulosic hydrolysate by candida guilliermondii fti 20037
Journal of Industrial and Engineering Chemistry, 2017Co-Authors: Priscila Vaz De Arruda, Julio Cesar Dos Santos, Rita C L B Rodrigues, Debora Danielle Virginio Da Silva, Celina Kiyomi Yamakawa, George Jackson De Moraes Rocha, Jonas Nolasco, Jose Geraldo Da Cruz Pradella, Carlos Eduardo Vaz RossellAbstract:Abstract In this study, volumetric oxygen mass transfer coefficient (kLa) was selected as a criterion for facilitating the scale up of xylitol production by Candida guilliermondii at the bench and pilot-scale level. A kLa value of 16 h−1 was applied in reactors with volumetric capacity of 2.4 L, 18 L and 125 L. Fermentation was successfully scaled-up from the bench to pilot-scale level with all experiments demonstrating a minimum of 60% xylose to xylitol conversion efficiency. Under all evaluated conditions glycerol and ethanol were also produced as by-products of xylose metabolism. Only minor differences were observed in the fermentation profile when reactor volumes ranging from 2.4 L to 125 L were used for experimentation purposes, reaching, at pilot scale, yield and volumetric productivity of 0.55 g g−1 and 0.31 g L−1 h−1, respectively, with maximum specific growth rate of 0.26 h−1. This demonstrates and reinforces the feasibility of using kLa as scale up criterion. The use of this parameter allowed precise reproduction of results obtained at bench bioreactor level to a larger scale; this is extremely crucial and important information considering that the aim of the proposed Biotechnological Process is to reach the level required for the industrial viability.
Chiara Schiraldi - One of the best experts on this subject based on the ideXlab platform.
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Boosted large-scale production and purification of a thermostable archaeal phosphotriesterase-like lactonase for organophosphate decontamination
Journal of Industrial Microbiology & Biotechnology, 2017Co-Authors: Odile Francesca Restaino, Mario De Rosa, Maria Giovanna Borzacchiello, Ilaria Scognamiglio, Elena Porzio, Giuseppe Manco, Luigi Fedele, Cinzia Donatiello, Chiara SchiraldiAbstract:Thermostable phosphotriesterase-like lactonases (PLLs) from extremophile archaea, like Sso Pox from Sulfolobus solfataricus , are attractive Biotechnological tools with industrial applications as organophosphate decontaminants, but their manufacturing still remains an unresolved issue because of the high costs and the low production yields. In this paper, for the first time, an efficient Biotechnological Process for the production and purification of a recombinant, engineered PLL, Sso Pox W263F, expressed in E. coli , has been set up by studying new induction strategies, by designing high cell density cultivations and a new membrane-based downstream Process. In fed batches, the enzyme production was boosted of 69-fold up to 4660.0 U L^−1 using galactose as inducer in the replacement of IPTG; the Process was scalable from 2.5 up to 150 L. By coupling a single thermo-precipitation step and an ultrafiltration Process, a total enzyme recovery of 77% with a purity grade of almost 80% was reached.
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high cell density cultivation of escherichia coli k4 in a microfiltration bioreactor a step towards improvement of chondroitin precursor production
Microbial Cell Factories, 2011Co-Authors: Odile Francesca Restaino, Donatella Cimini, Mario De Rosa, Angela Catapano, Chiara SchiraldiAbstract:Background The bacteria Escherichia coli K4 produces a capsular polysaccharide (K4 CPS) whose backbone is similar to the non sulphated chondroitin chain. The chondroitin sulphate is one of the major components of the extra-cellular matrix of the vertebrate connective tissues and a high value molecule, widely employed as active principle in the treatment of osteoarthritis. It is usually obtained by extraction from animal tissues, but the risk of virus contaminations, as well as the scarceness of raw material, makes this productive Process unsafe and unable to satisfy the growing market demand. In previous studies a new Biotechnological Process to produce chondroitin from Escherichia coli K4 capsular polysaccharide was investigated and a 1.4 g·L-1 K4 CPS concentration was reached using fed-batch fermentation techniques. In this work, on the trail of these results, we exploited new fermentation strategies to further improve the capsular polysaccharide production.
Talita L. Honorato - One of the best experts on this subject based on the ideXlab platform.
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Dextransucrase Stability in Cashew Apple Juice
'Springer Science and Business Media LLC', 2015Co-Authors: Talita L. Honorato, Rodrigues SAbstract:Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)The use of agriculture waste as substrate for Biotechnological Process became an interesting alternative to reduce production costs and the negative impact of agricultural wastes disposal in the environment. In Brazil, cashew tree cultivation is done aiming the cashew nut production, and tons of cashew apples are wasted in the field after the nut removal. Thus, cashew apple juice can be used as low cost substrate for Biotechnological Process. In this study, the stability of dextransucrase produced in a fermentation medium using cashew apple juice as substrate was evaluated. The crude enzyme was stable at 30 degrees C for 30 h at pH ranging from 4.5 to 5.5. The partially purified enzyme was stable in non-fermented cashew apple juice at pH 5.0 for 96 h at 30 degrees C. The high stability of the enzyme in cashew apple juice at room temperature allows its industrial use without needing the enzyme purification, consequently reducing Process costs.31105110Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq
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Dextransucrase Stability in Cashew Apple Juice
Food and Bioprocess Technology, 2008Co-Authors: Talita L. Honorato, Sueli RodriguesAbstract:The use of agriculture waste as substrate for Biotechnological Process became an interesting alternative to reduce production costs and the negative impact of agricultural wastes disposal in the environment. In Brazil, cashew tree cultivation is done aiming the cashew nut production, and tons of cashew apples are wasted in the field after the nut removal. Thus, cashew apple juice can be used as low cost substrate for Biotechnological Process. In this study, the stability of dextransucrase produced in a fermentation medium using cashew apple juice as substrate was evaluated. The crude enzyme was stable at 30 °C for 30 h at pH ranging from 4.5 to 5.5. The partially purified enzyme was stable in non-fermented cashew apple juice at pH 5.0 for 96 h at 30 °C. The high stability of the enzyme in cashew apple juice at room temperature allows its industrial use without needing the enzyme purification, consequently reducing Process costs.