The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform

Lília R. Almeida - One of the best experts on this subject based on the ideXlab platform.

  • Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications.
    Journal of tissue engineering and regenerative medicine, 2016
    Co-Authors: Viviana P. Ribeiro, Lília R. Almeida, A. R. Martins, Alexandra P. Marques, Iva Pashkuleva, Graça Bonifácio, Rui A. Sousa, Ana S. Ribeiro, Carla J. S. M. Silva, Ana L. Oliveira
    Abstract:

    Textile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineering applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the scaffolds developed: sodium hydroxide etching, ultraviolet radiation exposure in an ozone atmosphere and grafting (acrylic acid, vinyl phosphonic acid and vinyl sulphonic acid) after oxygen plasma activation as a way to tailor cell adhesion. A possible effect of the applied treatments on the bulk properties of the textile scaffolds has been considered and thus tensile tests in dry and hydrated states were also carried out. The microscopy results indicated that the surface morphology and roughness were affected by the applied treatments. The X-ray photoelectron spectroscopy and contact angle measurements showed the incorporation of oxygen-containing groups and higher surface free energy as result of the surface treatments applied. The DNA quantification and scanning electron microscopy analysis revealed that these modifications enhanced cell adhesion and altered cell morphology. Generally, sodium hydroxide treatment altered most significantly the surface properties, which in turn resulted in a high number of cells adherent to these surfaces. Based on the results obtained, the proposed surface treatments are appropriate to modify polybutylene succinate knitting scaffolds, influencing cell adhesion and its potential for use in tissue engineering applications. Copyright © 2016 John Wiley & Sons, Ltd.

  • Influence of different surface modification treatments on silk Biotextiles for tissue engineering applications.
    Journal of biomedical materials research. Part B Applied biomaterials, 2015
    Co-Authors: Viviana P. Ribeiro, Lília R. Almeida, A. R. Martins, Alexandra P. Marques, Iva Pashkuleva, Graça Bonifácio, Rui A. Sousa, Ana S. Ribeiro, Carla J. S. M. Silva, Rui L. Reis
    Abstract:

    Contract grant sponsor: Portuguese Foundation for Science and Technology under POCTI and/or FEDER programs under the scope of the project TISSUE2TISSUE; contract grant number: PTDC/CTM/105703/2008Contract grant sponsor: Investigator FCT program (to A.N.L.); contract grant number: IF/00411/2013

  • errata to new Biotextiles for tissue engineering development characterization and in vitro cellular viability acta biomaterialia 9 2013 8167 8181
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Alexandra P. Marques, Iva Pashkuleva, João F. Mano, Ana Bela Sarmento Ribeiro, Nelson F. Durães
    Abstract:

    Errata to: ‘‘New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability’’ [Acta Biomaterialia 9 (2013) 8167–8181] Lilia R. Almeida , Ana R. Martins , Emanuel M. Fernandes , Mariana B. Oliveira , Joao F. Mano , Vitor M. Correlo , Iva Pashkuleva , Alexandra P. Marques , Ana S. Ribeiro , Nelson F. Duraes , Carla J. Silva , Graca Bonifacio , Rui A. Sousa , Ana L. Oliveira , Rui L. Reis a,b a 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Portugal b ICVS/3B’s – PT Government Associated Laboratory, Braga/Guimaraes, Portugal CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicao, Portugal CITEVE, Technological Centre for Textile and Clothing Industry, V.N. Famalicao, Portugal Department of Health Sciences, Portuguese Catholic University, Viseu, Portugal

  • New Biotextiles for tissue engineering: development, characterization and in vitro cellular viability.
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Ana Sofia Ribeiro, Nelson F. Durães, Alexandra P. Marques, Iva Pashkuleva, Carla J. Silva
    Abstract:

    This work proposes biodegradable textile-based structures for tissue engineering applications. We describe the use of two polymers, polybutylene succinate (PBS) proposed as a viable multifilamentand silk fibroin (SF), to produce fibre-based finely tuned porous architectures by weft knitting. PBS is here proposed as a viable extruded multifilament fibre to be processed by a textile-based technology. A comparative study was undertaken using a SF fibre with a similar linear density. The knitted constructs obtained are described in terms of their morphology, mechanical properties, swelling capability, degradation behaviour and cytotoxicity. The weft knitting technology used offers superior control over the scaffold design (e.g. size, shape, porosity and fibre alignment), manufacturing and reproducibility. The presented fibres allow the processing of a very reproducible intra-architectural scaffold geometry which is fully interconnected, thus providing a high surface area for cell attachment and tissue in-growth. The two types of polymer fibre allow the generation of constructs with distinct characteristics in terms of the surface physico-chemistry, mechanical performance and degradation capability, which has an impact on the resulting cell behaviour at the surface of the respective Biotextiles. Preliminary cytotoxicity screening showed that both materials can support cell adhesion and proliferation. These results constitute a first validation of the two Biotextiles as viable matrices for tissue engineering prior to the development of more complex systems. Given the processing efficacy and versatility of the knitting technology and the interesting structural and surface properties of the proposed polymer fibres it is foreseen that the developed systems could be attractive for the functional engineering of tissues such as skin, ligament, bone or cartilage.

  • Errata to: “New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability” [Acta Biomaterialia 9 (2013) 8167–8181]
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Ana Sofia Ribeiro, Alexandra P. Marques, Iva Pashkuleva, João F. Mano, Nelson F. Durães
    Abstract:

    Errata to: ‘‘New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability’’ [Acta Biomaterialia 9 (2013) 8167–8181] Lilia R. Almeida , Ana R. Martins , Emanuel M. Fernandes , Mariana B. Oliveira , Joao F. Mano , Vitor M. Correlo , Iva Pashkuleva , Alexandra P. Marques , Ana S. Ribeiro , Nelson F. Duraes , Carla J. Silva , Graca Bonifacio , Rui A. Sousa , Ana L. Oliveira , Rui L. Reis a,b a 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Portugal b ICVS/3B’s – PT Government Associated Laboratory, Braga/Guimaraes, Portugal CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicao, Portugal CITEVE, Technological Centre for Textile and Clothing Industry, V.N. Famalicao, Portugal Department of Health Sciences, Portuguese Catholic University, Viseu, Portugal

Alexandra P. Marques - One of the best experts on this subject based on the ideXlab platform.

  • Silk-based anisotropical 3D Biotextiles for bone regeneration
    Biomaterials, 2017
    Co-Authors: Viviana P. Ribeiro, Alexandra P. Marques, Graça Bonifácio, Rui A. Sousa, Joana Silva-correia, Ana Isabel Delgado Cravo Lopes Nascimento, Alain Da Silva Morais, Ana S. Ribeiro, Carla J. S. M. Silva, Joaquim M. Oliveira
    Abstract:

    Bone loss in the craniofacial complex can been treated using several conventional therapeutic strategies that face many obstacles and limitations. In this work, novel three-dimensional (3D) biotextile architectures were developed as a possible strategy for flat bone regeneration applications. As a fully automated processing route, this strategy as potential to be easily industrialized. Silk fibroin (SF) yarns were processed into weft-knitted fabrics spaced by a monofilament of polyethylene terephthalate (PET). A comparative study with a similar 3D structure made entirely of PET was established. Highly porous scaffolds with homogeneous pore distribution were observed using micro-computed tomography analysis. The wet state dynamic mechanical analysis revealed a storage modulus In the frequency range tested, the storage modulus values obtained for SF-PET scaffolds were higher than for the PET scaffolds. Human adipose-derived stem cells (hASCs) cultured on the SF-PET spacer structures showed the typical pattern for ALP activity under osteogenic culture conditions. Osteogenic differentiation of hASCs on SF-PET and PET constructs was also observed by extracellular matrix mineralization and expression of osteogenic-related markers (osteocalcin, osteopontin and collagen type I) after 28 days of osteogenic culture, in comparison to the control basal medium. The quantification of convergent macroscopic blood vessels toward the scaffolds by a chick chorioallantoic membrane assay, showed higher angiogenic response induced by the SF-PET textile scaffolds than PET structures and gelatin sponge controls. Subcutaneous implantation in CD-1 mice revealed tissue ingrowth's accompanied by blood vessels infiltration in both spacer constructs. The structural adaptability of textile structures combined to the structural similarities of the 3D knitted spacer fabrics to craniofacial bone tissue and achieved biological performance, make these scaffolds a possible solution for tissue engineering approaches in this area.

  • Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications.
    Journal of tissue engineering and regenerative medicine, 2016
    Co-Authors: Viviana P. Ribeiro, Lília R. Almeida, A. R. Martins, Alexandra P. Marques, Iva Pashkuleva, Graça Bonifácio, Rui A. Sousa, Ana S. Ribeiro, Carla J. S. M. Silva, Ana L. Oliveira
    Abstract:

    Textile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineering applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the scaffolds developed: sodium hydroxide etching, ultraviolet radiation exposure in an ozone atmosphere and grafting (acrylic acid, vinyl phosphonic acid and vinyl sulphonic acid) after oxygen plasma activation as a way to tailor cell adhesion. A possible effect of the applied treatments on the bulk properties of the textile scaffolds has been considered and thus tensile tests in dry and hydrated states were also carried out. The microscopy results indicated that the surface morphology and roughness were affected by the applied treatments. The X-ray photoelectron spectroscopy and contact angle measurements showed the incorporation of oxygen-containing groups and higher surface free energy as result of the surface treatments applied. The DNA quantification and scanning electron microscopy analysis revealed that these modifications enhanced cell adhesion and altered cell morphology. Generally, sodium hydroxide treatment altered most significantly the surface properties, which in turn resulted in a high number of cells adherent to these surfaces. Based on the results obtained, the proposed surface treatments are appropriate to modify polybutylene succinate knitting scaffolds, influencing cell adhesion and its potential for use in tissue engineering applications. Copyright © 2016 John Wiley & Sons, Ltd.

  • Influence of different surface modification treatments on silk Biotextiles for tissue engineering applications.
    Journal of biomedical materials research. Part B Applied biomaterials, 2015
    Co-Authors: Viviana P. Ribeiro, Lília R. Almeida, A. R. Martins, Alexandra P. Marques, Iva Pashkuleva, Graça Bonifácio, Rui A. Sousa, Ana S. Ribeiro, Carla J. S. M. Silva, Rui L. Reis
    Abstract:

    Contract grant sponsor: Portuguese Foundation for Science and Technology under POCTI and/or FEDER programs under the scope of the project TISSUE2TISSUE; contract grant number: PTDC/CTM/105703/2008Contract grant sponsor: Investigator FCT program (to A.N.L.); contract grant number: IF/00411/2013

  • errata to new Biotextiles for tissue engineering development characterization and in vitro cellular viability acta biomaterialia 9 2013 8167 8181
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Alexandra P. Marques, Iva Pashkuleva, João F. Mano, Ana Bela Sarmento Ribeiro, Nelson F. Durães
    Abstract:

    Errata to: ‘‘New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability’’ [Acta Biomaterialia 9 (2013) 8167–8181] Lilia R. Almeida , Ana R. Martins , Emanuel M. Fernandes , Mariana B. Oliveira , Joao F. Mano , Vitor M. Correlo , Iva Pashkuleva , Alexandra P. Marques , Ana S. Ribeiro , Nelson F. Duraes , Carla J. Silva , Graca Bonifacio , Rui A. Sousa , Ana L. Oliveira , Rui L. Reis a,b a 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Portugal b ICVS/3B’s – PT Government Associated Laboratory, Braga/Guimaraes, Portugal CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicao, Portugal CITEVE, Technological Centre for Textile and Clothing Industry, V.N. Famalicao, Portugal Department of Health Sciences, Portuguese Catholic University, Viseu, Portugal

  • New Biotextiles for tissue engineering: development, characterization and in vitro cellular viability.
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Ana Sofia Ribeiro, Nelson F. Durães, Alexandra P. Marques, Iva Pashkuleva, Carla J. Silva
    Abstract:

    This work proposes biodegradable textile-based structures for tissue engineering applications. We describe the use of two polymers, polybutylene succinate (PBS) proposed as a viable multifilamentand silk fibroin (SF), to produce fibre-based finely tuned porous architectures by weft knitting. PBS is here proposed as a viable extruded multifilament fibre to be processed by a textile-based technology. A comparative study was undertaken using a SF fibre with a similar linear density. The knitted constructs obtained are described in terms of their morphology, mechanical properties, swelling capability, degradation behaviour and cytotoxicity. The weft knitting technology used offers superior control over the scaffold design (e.g. size, shape, porosity and fibre alignment), manufacturing and reproducibility. The presented fibres allow the processing of a very reproducible intra-architectural scaffold geometry which is fully interconnected, thus providing a high surface area for cell attachment and tissue in-growth. The two types of polymer fibre allow the generation of constructs with distinct characteristics in terms of the surface physico-chemistry, mechanical performance and degradation capability, which has an impact on the resulting cell behaviour at the surface of the respective Biotextiles. Preliminary cytotoxicity screening showed that both materials can support cell adhesion and proliferation. These results constitute a first validation of the two Biotextiles as viable matrices for tissue engineering prior to the development of more complex systems. Given the processing efficacy and versatility of the knitting technology and the interesting structural and surface properties of the proposed polymer fibres it is foreseen that the developed systems could be attractive for the functional engineering of tissues such as skin, ligament, bone or cartilage.

Nelson F. Durães - One of the best experts on this subject based on the ideXlab platform.

  • errata to new Biotextiles for tissue engineering development characterization and in vitro cellular viability acta biomaterialia 9 2013 8167 8181
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Alexandra P. Marques, Iva Pashkuleva, João F. Mano, Ana Bela Sarmento Ribeiro, Nelson F. Durães
    Abstract:

    Errata to: ‘‘New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability’’ [Acta Biomaterialia 9 (2013) 8167–8181] Lilia R. Almeida , Ana R. Martins , Emanuel M. Fernandes , Mariana B. Oliveira , Joao F. Mano , Vitor M. Correlo , Iva Pashkuleva , Alexandra P. Marques , Ana S. Ribeiro , Nelson F. Duraes , Carla J. Silva , Graca Bonifacio , Rui A. Sousa , Ana L. Oliveira , Rui L. Reis a,b a 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Portugal b ICVS/3B’s – PT Government Associated Laboratory, Braga/Guimaraes, Portugal CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicao, Portugal CITEVE, Technological Centre for Textile and Clothing Industry, V.N. Famalicao, Portugal Department of Health Sciences, Portuguese Catholic University, Viseu, Portugal

  • New Biotextiles for tissue engineering: development, characterization and in vitro cellular viability.
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Ana Sofia Ribeiro, Nelson F. Durães, Alexandra P. Marques, Iva Pashkuleva, Carla J. Silva
    Abstract:

    This work proposes biodegradable textile-based structures for tissue engineering applications. We describe the use of two polymers, polybutylene succinate (PBS) proposed as a viable multifilamentand silk fibroin (SF), to produce fibre-based finely tuned porous architectures by weft knitting. PBS is here proposed as a viable extruded multifilament fibre to be processed by a textile-based technology. A comparative study was undertaken using a SF fibre with a similar linear density. The knitted constructs obtained are described in terms of their morphology, mechanical properties, swelling capability, degradation behaviour and cytotoxicity. The weft knitting technology used offers superior control over the scaffold design (e.g. size, shape, porosity and fibre alignment), manufacturing and reproducibility. The presented fibres allow the processing of a very reproducible intra-architectural scaffold geometry which is fully interconnected, thus providing a high surface area for cell attachment and tissue in-growth. The two types of polymer fibre allow the generation of constructs with distinct characteristics in terms of the surface physico-chemistry, mechanical performance and degradation capability, which has an impact on the resulting cell behaviour at the surface of the respective Biotextiles. Preliminary cytotoxicity screening showed that both materials can support cell adhesion and proliferation. These results constitute a first validation of the two Biotextiles as viable matrices for tissue engineering prior to the development of more complex systems. Given the processing efficacy and versatility of the knitting technology and the interesting structural and surface properties of the proposed polymer fibres it is foreseen that the developed systems could be attractive for the functional engineering of tissues such as skin, ligament, bone or cartilage.

  • Evaluation of Novel 3D Architectures Based on Knitting Technologies for Engineering Biological Tissues
    Journal of Donghua University, 2013
    Co-Authors: Viviana P. Ribeiro, Vitor M. Correlo, Ana Sofia Ribeiro, Nelson F. Durães, Carla J. Silva, Alexandra P. Marques, Graça Bonifácio, Rui A. Sousa, Ana L. Oliveira, Rui L. Reis
    Abstract:

    Textile-based technologies are considered as potential routes for the production of 3D porous architectures for tissue engineering( TE) applications. We describe the use of two polymers,namely polybutylene succinate( PBS) and silk fibroin(SF) to produce fiber-based finely tuned porous architectures by weft and warp knittings. The obtained knitted constructs are described in terms of their morphology, mechanical properties,swelling ability,degradation behaviour,and cytotoxicity. Each type of polymer fibers allows for the processing of a very reproducible intra-architectural scaffold geometry,with distinct characteristics in terms of the surface physicochemistry,mechanical performance,and degradation capability,which has an impact on the resulting cell behaviour at the surface of the respective Biotextiles. Preliminary cytotoxicity screening shows that both materials can support cell adhesion and proliferation. Furthermore, different surface modifications were performed( acid /alkaline treatment, UV radiation,and plasma) for modulating cell behavior. An increase of cell-material interactions were observed,indicating the important role of materials surface in the first hours of culturing. Human adipose-derived stem cells( hASCs) became an emerging possibility for regenerative medicine and tissue replacement therapies. The potential of the recently developed silk-based biotextile structures to promote hASCs adhesion,proliferation,and differentiation is also evaluated. The obtained results validate the developed constructs as viable matrices for TE applications. Given the processing efficacy and versatility of the knitting technology, and the interesting structural and surface properties of the proposed polymer fibers,it is foreseen that our developed systems can be attractive for the functional engineering of tissues such as bone,skin,ligaments or cartilage and also for develop more complex systems for further industrialization of TE products.

  • Errata to: “New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability” [Acta Biomaterialia 9 (2013) 8167–8181]
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Ana Sofia Ribeiro, Alexandra P. Marques, Iva Pashkuleva, João F. Mano, Nelson F. Durães
    Abstract:

    Errata to: ‘‘New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability’’ [Acta Biomaterialia 9 (2013) 8167–8181] Lilia R. Almeida , Ana R. Martins , Emanuel M. Fernandes , Mariana B. Oliveira , Joao F. Mano , Vitor M. Correlo , Iva Pashkuleva , Alexandra P. Marques , Ana S. Ribeiro , Nelson F. Duraes , Carla J. Silva , Graca Bonifacio , Rui A. Sousa , Ana L. Oliveira , Rui L. Reis a,b a 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Portugal b ICVS/3B’s – PT Government Associated Laboratory, Braga/Guimaraes, Portugal CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicao, Portugal CITEVE, Technological Centre for Textile and Clothing Industry, V.N. Famalicao, Portugal Department of Health Sciences, Portuguese Catholic University, Viseu, Portugal

A. R. Martins - One of the best experts on this subject based on the ideXlab platform.

  • Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications.
    Journal of tissue engineering and regenerative medicine, 2016
    Co-Authors: Viviana P. Ribeiro, Lília R. Almeida, A. R. Martins, Alexandra P. Marques, Iva Pashkuleva, Graça Bonifácio, Rui A. Sousa, Ana S. Ribeiro, Carla J. S. M. Silva, Ana L. Oliveira
    Abstract:

    Textile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineering applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the scaffolds developed: sodium hydroxide etching, ultraviolet radiation exposure in an ozone atmosphere and grafting (acrylic acid, vinyl phosphonic acid and vinyl sulphonic acid) after oxygen plasma activation as a way to tailor cell adhesion. A possible effect of the applied treatments on the bulk properties of the textile scaffolds has been considered and thus tensile tests in dry and hydrated states were also carried out. The microscopy results indicated that the surface morphology and roughness were affected by the applied treatments. The X-ray photoelectron spectroscopy and contact angle measurements showed the incorporation of oxygen-containing groups and higher surface free energy as result of the surface treatments applied. The DNA quantification and scanning electron microscopy analysis revealed that these modifications enhanced cell adhesion and altered cell morphology. Generally, sodium hydroxide treatment altered most significantly the surface properties, which in turn resulted in a high number of cells adherent to these surfaces. Based on the results obtained, the proposed surface treatments are appropriate to modify polybutylene succinate knitting scaffolds, influencing cell adhesion and its potential for use in tissue engineering applications. Copyright © 2016 John Wiley & Sons, Ltd.

  • Influence of different surface modification treatments on silk Biotextiles for tissue engineering applications.
    Journal of biomedical materials research. Part B Applied biomaterials, 2015
    Co-Authors: Viviana P. Ribeiro, Lília R. Almeida, A. R. Martins, Alexandra P. Marques, Iva Pashkuleva, Graça Bonifácio, Rui A. Sousa, Ana S. Ribeiro, Carla J. S. M. Silva, Rui L. Reis
    Abstract:

    Contract grant sponsor: Portuguese Foundation for Science and Technology under POCTI and/or FEDER programs under the scope of the project TISSUE2TISSUE; contract grant number: PTDC/CTM/105703/2008Contract grant sponsor: Investigator FCT program (to A.N.L.); contract grant number: IF/00411/2013

  • errata to new Biotextiles for tissue engineering development characterization and in vitro cellular viability acta biomaterialia 9 2013 8167 8181
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Alexandra P. Marques, Iva Pashkuleva, João F. Mano, Ana Bela Sarmento Ribeiro, Nelson F. Durães
    Abstract:

    Errata to: ‘‘New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability’’ [Acta Biomaterialia 9 (2013) 8167–8181] Lilia R. Almeida , Ana R. Martins , Emanuel M. Fernandes , Mariana B. Oliveira , Joao F. Mano , Vitor M. Correlo , Iva Pashkuleva , Alexandra P. Marques , Ana S. Ribeiro , Nelson F. Duraes , Carla J. Silva , Graca Bonifacio , Rui A. Sousa , Ana L. Oliveira , Rui L. Reis a,b a 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Portugal b ICVS/3B’s – PT Government Associated Laboratory, Braga/Guimaraes, Portugal CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicao, Portugal CITEVE, Technological Centre for Textile and Clothing Industry, V.N. Famalicao, Portugal Department of Health Sciences, Portuguese Catholic University, Viseu, Portugal

  • New Biotextiles for tissue engineering: development, characterization and in vitro cellular viability.
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Ana Sofia Ribeiro, Nelson F. Durães, Alexandra P. Marques, Iva Pashkuleva, Carla J. Silva
    Abstract:

    This work proposes biodegradable textile-based structures for tissue engineering applications. We describe the use of two polymers, polybutylene succinate (PBS) proposed as a viable multifilamentand silk fibroin (SF), to produce fibre-based finely tuned porous architectures by weft knitting. PBS is here proposed as a viable extruded multifilament fibre to be processed by a textile-based technology. A comparative study was undertaken using a SF fibre with a similar linear density. The knitted constructs obtained are described in terms of their morphology, mechanical properties, swelling capability, degradation behaviour and cytotoxicity. The weft knitting technology used offers superior control over the scaffold design (e.g. size, shape, porosity and fibre alignment), manufacturing and reproducibility. The presented fibres allow the processing of a very reproducible intra-architectural scaffold geometry which is fully interconnected, thus providing a high surface area for cell attachment and tissue in-growth. The two types of polymer fibre allow the generation of constructs with distinct characteristics in terms of the surface physico-chemistry, mechanical performance and degradation capability, which has an impact on the resulting cell behaviour at the surface of the respective Biotextiles. Preliminary cytotoxicity screening showed that both materials can support cell adhesion and proliferation. These results constitute a first validation of the two Biotextiles as viable matrices for tissue engineering prior to the development of more complex systems. Given the processing efficacy and versatility of the knitting technology and the interesting structural and surface properties of the proposed polymer fibres it is foreseen that the developed systems could be attractive for the functional engineering of tissues such as skin, ligament, bone or cartilage.

  • Errata to: “New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability” [Acta Biomaterialia 9 (2013) 8167–8181]
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Ana Sofia Ribeiro, Alexandra P. Marques, Iva Pashkuleva, João F. Mano, Nelson F. Durães
    Abstract:

    Errata to: ‘‘New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability’’ [Acta Biomaterialia 9 (2013) 8167–8181] Lilia R. Almeida , Ana R. Martins , Emanuel M. Fernandes , Mariana B. Oliveira , Joao F. Mano , Vitor M. Correlo , Iva Pashkuleva , Alexandra P. Marques , Ana S. Ribeiro , Nelson F. Duraes , Carla J. Silva , Graca Bonifacio , Rui A. Sousa , Ana L. Oliveira , Rui L. Reis a,b a 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Portugal b ICVS/3B’s – PT Government Associated Laboratory, Braga/Guimaraes, Portugal CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicao, Portugal CITEVE, Technological Centre for Textile and Clothing Industry, V.N. Famalicao, Portugal Department of Health Sciences, Portuguese Catholic University, Viseu, Portugal

Iva Pashkuleva - One of the best experts on this subject based on the ideXlab platform.

  • Modulating cell adhesion to polybutylene succinate biotextile constructs for tissue engineering applications.
    Journal of tissue engineering and regenerative medicine, 2016
    Co-Authors: Viviana P. Ribeiro, Lília R. Almeida, A. R. Martins, Alexandra P. Marques, Iva Pashkuleva, Graça Bonifácio, Rui A. Sousa, Ana S. Ribeiro, Carla J. S. M. Silva, Ana L. Oliveira
    Abstract:

    Textile-based technologies are powerful routes for the production of three-dimensional porous architectures for tissue engineering applications because of their feasibility and possibility for scaling-up. Herein, the use of knitting technology to produce polybutylene succinate fibre-based porous architectures is described. Furthermore, different treatments have been applied to functionalize the surface of the scaffolds developed: sodium hydroxide etching, ultraviolet radiation exposure in an ozone atmosphere and grafting (acrylic acid, vinyl phosphonic acid and vinyl sulphonic acid) after oxygen plasma activation as a way to tailor cell adhesion. A possible effect of the applied treatments on the bulk properties of the textile scaffolds has been considered and thus tensile tests in dry and hydrated states were also carried out. The microscopy results indicated that the surface morphology and roughness were affected by the applied treatments. The X-ray photoelectron spectroscopy and contact angle measurements showed the incorporation of oxygen-containing groups and higher surface free energy as result of the surface treatments applied. The DNA quantification and scanning electron microscopy analysis revealed that these modifications enhanced cell adhesion and altered cell morphology. Generally, sodium hydroxide treatment altered most significantly the surface properties, which in turn resulted in a high number of cells adherent to these surfaces. Based on the results obtained, the proposed surface treatments are appropriate to modify polybutylene succinate knitting scaffolds, influencing cell adhesion and its potential for use in tissue engineering applications. Copyright © 2016 John Wiley & Sons, Ltd.

  • Influence of different surface modification treatments on silk Biotextiles for tissue engineering applications.
    Journal of biomedical materials research. Part B Applied biomaterials, 2015
    Co-Authors: Viviana P. Ribeiro, Lília R. Almeida, A. R. Martins, Alexandra P. Marques, Iva Pashkuleva, Graça Bonifácio, Rui A. Sousa, Ana S. Ribeiro, Carla J. S. M. Silva, Rui L. Reis
    Abstract:

    Contract grant sponsor: Portuguese Foundation for Science and Technology under POCTI and/or FEDER programs under the scope of the project TISSUE2TISSUE; contract grant number: PTDC/CTM/105703/2008Contract grant sponsor: Investigator FCT program (to A.N.L.); contract grant number: IF/00411/2013

  • errata to new Biotextiles for tissue engineering development characterization and in vitro cellular viability acta biomaterialia 9 2013 8167 8181
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Alexandra P. Marques, Iva Pashkuleva, João F. Mano, Ana Bela Sarmento Ribeiro, Nelson F. Durães
    Abstract:

    Errata to: ‘‘New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability’’ [Acta Biomaterialia 9 (2013) 8167–8181] Lilia R. Almeida , Ana R. Martins , Emanuel M. Fernandes , Mariana B. Oliveira , Joao F. Mano , Vitor M. Correlo , Iva Pashkuleva , Alexandra P. Marques , Ana S. Ribeiro , Nelson F. Duraes , Carla J. Silva , Graca Bonifacio , Rui A. Sousa , Ana L. Oliveira , Rui L. Reis a,b a 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Portugal b ICVS/3B’s – PT Government Associated Laboratory, Braga/Guimaraes, Portugal CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicao, Portugal CITEVE, Technological Centre for Textile and Clothing Industry, V.N. Famalicao, Portugal Department of Health Sciences, Portuguese Catholic University, Viseu, Portugal

  • New Biotextiles for tissue engineering: development, characterization and in vitro cellular viability.
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Ana Sofia Ribeiro, Nelson F. Durães, Alexandra P. Marques, Iva Pashkuleva, Carla J. Silva
    Abstract:

    This work proposes biodegradable textile-based structures for tissue engineering applications. We describe the use of two polymers, polybutylene succinate (PBS) proposed as a viable multifilamentand silk fibroin (SF), to produce fibre-based finely tuned porous architectures by weft knitting. PBS is here proposed as a viable extruded multifilament fibre to be processed by a textile-based technology. A comparative study was undertaken using a SF fibre with a similar linear density. The knitted constructs obtained are described in terms of their morphology, mechanical properties, swelling capability, degradation behaviour and cytotoxicity. The weft knitting technology used offers superior control over the scaffold design (e.g. size, shape, porosity and fibre alignment), manufacturing and reproducibility. The presented fibres allow the processing of a very reproducible intra-architectural scaffold geometry which is fully interconnected, thus providing a high surface area for cell attachment and tissue in-growth. The two types of polymer fibre allow the generation of constructs with distinct characteristics in terms of the surface physico-chemistry, mechanical performance and degradation capability, which has an impact on the resulting cell behaviour at the surface of the respective Biotextiles. Preliminary cytotoxicity screening showed that both materials can support cell adhesion and proliferation. These results constitute a first validation of the two Biotextiles as viable matrices for tissue engineering prior to the development of more complex systems. Given the processing efficacy and versatility of the knitting technology and the interesting structural and surface properties of the proposed polymer fibres it is foreseen that the developed systems could be attractive for the functional engineering of tissues such as skin, ligament, bone or cartilage.

  • Errata to: “New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability” [Acta Biomaterialia 9 (2013) 8167–8181]
    Acta Biomaterialia, 2013
    Co-Authors: Lília R. Almeida, A. R. Martins, Emanuel M. Fernandes, Mariana B. Oliveira, Vitor M. Correlo, Ana Sofia Ribeiro, Alexandra P. Marques, Iva Pashkuleva, João F. Mano, Nelson F. Durães
    Abstract:

    Errata to: ‘‘New Biotextiles for tissue engineering: Development, characterization and in vitro cellular viability’’ [Acta Biomaterialia 9 (2013) 8167–8181] Lilia R. Almeida , Ana R. Martins , Emanuel M. Fernandes , Mariana B. Oliveira , Joao F. Mano , Vitor M. Correlo , Iva Pashkuleva , Alexandra P. Marques , Ana S. Ribeiro , Nelson F. Duraes , Carla J. Silva , Graca Bonifacio , Rui A. Sousa , Ana L. Oliveira , Rui L. Reis a,b a 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909 Caldas das Taipas, Portugal b ICVS/3B’s – PT Government Associated Laboratory, Braga/Guimaraes, Portugal CeNTI, Centre for Nanotechnology and Smart Materials, V.N. Famalicao, Portugal CITEVE, Technological Centre for Textile and Clothing Industry, V.N. Famalicao, Portugal Department of Health Sciences, Portuguese Catholic University, Viseu, Portugal