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Shai Morin - One of the best experts on this subject based on the ideXlab platform.

  • evidence for pre zygotic reproductive barrier between the b and q Biotypes of bemisia tabaci hemiptera aleyrodidae
    Bulletin of Entomological Research, 2010
    Co-Authors: Moshe Elbaz, N Lahav, Shai Morin
    Abstract:

    : The degree of reproductive isolation between the B and Q Biotypes of the whitefly Bemisia tabaci (Hemiptera: Aleyrodidae) is currently not clear. Laboratory experiments have shown that the two Biotypes are capable of producing viable F1 hybrids but that these females are sterile as their F2 generation failed to develop, indicating, most likely, a post-zygotic reproductive barrier. Here, we confirm, by molecular and ecological tools, that the B and Q Biotypes of Israel are genetically isolated and provide two independent lines of evidence that support the existence of a pre-zygotic reproductive barrier between them. Firstly, monitoring of mating behaviors in homogeneous and heterogeneous couples indicated no copulation events in heterogeneous couples compared to approximately 50% in homogeneous B and Q couples. Secondly, we could not detect the presence of sperm in the spermathecae of females from heterogeneous couples, compared to 50% detection in intra-B Biotype crosses and 15% detection in intra-Q Biotype crosses. The existence of pre-zygotic reproductive barriers in Israeli B and Q colonies may indicate a reinforcement process in which mating discrimination is strengthened between sympatric taxa that were formerly allopatric, to avoid maladaptive hybridization. As the two Biotypes continued to perform all courtship stages prior to copulation, we also conducted mixed cultures experiments in order to test the reproductive consequences of inter-Biotype courtship attempts. In mixed cultures, a significant reduction in female fecundity was observed for the Q Biotype but not for the B Biotype, suggesting an asymmetric reproductive interference effect in favour of the B Biotype. The long-term outcome of this effect is yet to be determined since additional environmental forces may reduce the probability of demographic displacement of one Biotype by the other in overlapping niches.

  • multiple origins of pyrethroid resistance in sympatric Biotypes of bemisia tabaci hemiptera aleyrodidae
    Insect Biochemistry and Molecular Biology, 2006
    Co-Authors: Michal Alon, Juergen Benting, Bettina Lueke, Tanja Ponge, Fishel Alon, Shai Morin
    Abstract:

    Abstract The two most damaging Biotypes of Bemisia tabaci , B and Q, are sympatric in the Mediterranean basin and show high resistance to pyrethroids synergized by organophosphates. Previous work showed that in the B Biotype, this resistance is associated with the L925I mutation in the para -type voltage gated sodium channel. Here we identified two mutations in the para -type voltage gated sodium channel associated with resistance to pyrethroids synergized by organophosphates in the Q Biotype: the L925I mutation that occurs in the B Biotype, and substitution of threonine to valine, at position 929 (T929V). To determine if the L925I and T929V mutations have single or multiple origins, we sequenced the DNA regions flanking the mutations from 13 B and Q strains collected worldwide. The survey identified five resistant alleles and five susceptible alleles. In the resistant alleles, the nucleotide diversity was low within Biotypes (0.001), but high between Biotypes (0.033). Nucleotide diversity in susceptible alleles was high between the two Biotypes (0.028). These observations are consistent with multiple independent origins of resistance. Although the B and Q Biotypes coexist in several regions of the Mediterranean basin, divergence in their DNA sequences at the para -type voltage gated sodium channel locus suggests gene flow between these Biotypes is low or nil.

  • dna markers for identifying Biotypes b and q of bemisia tabaci hemiptera aleyrodidae and studying population dynamics
    Bulletin of Entomological Research, 2005
    Co-Authors: V Khasdan, Svetlana Kontsedalov, Shai Morin, I Levin, A Rosner, L Maslenin, A. R. Horowitz
    Abstract:

    The two most widespread Biotypes of Bemisia tabaci (Gennadius) in southern Europe and the Middle East are referred to as the B and Q-type, which are morphologically indistinguishable. In this study various DNA markers have been developed, applied and compared for studying genetic diversity and distribution of the two Biotypes. For developing sequence characterized amplified regions (SCAR) and cleaved amplified polymorphic sequences (CAPS) techniques, single random amplified polymorphic DNA (RAPD) fragments of B and Q Biotypes, respectively, were used. The CAPS were investigated on the basis of nuclear sodium channel and the mitochondrial cytochrome oxidase I genes (mtCOI) sequences. In general, complete agreement was found between the different markers used. Analysis of field samples collected in Israel for several years, using these markers, indicated that the percentage of the Q Biotype tends to increase in field populations as time progresses. This may be attributed to the resistance of the Q Biotype to neonicotinoids and pyriproxyfen and the susceptibility of the B Biotype to these insecticides.

Carol A Mallorysmith - One of the best experts on this subject based on the ideXlab platform.

  • cross resistance of acetyl coa carboxylase accase inhibitor resistant wild oat avena fatua Biotypes in the pacific northwest
    Weed Technology, 2008
    Co-Authors: Ahmet Uludag, Kee Woong Park, Joshua Cannon, Carol A Mallorysmith
    Abstract:

    Seeds from five suspected acetyl-CoA carboxylase (ACCase) inhibitor–resistant wild oat Biotypes (R1 to R5) were collected in wheat and lentil fields in the Pacific Northwest. Based on whole plant dose–response experiments, the five resistant Biotypes were 2 to 24 times more resistant to the aryloxyphenoxypropionate (APP) herbicides (fenoxaprop, diclofop, and quizalofop) compared with the susceptible Biotype. However, none of the resistant Biotypes were resistant to the cyclohexanedione (CHD) herbicides, sethoxydim and clethodim. R2 was the only Biotype resistant to tralkoxydim and pinoxaden, a phenylpyrazolin herbicide and an ACCase inhibitor. The R2 Biotype was 35 and 16 times more resistant to tralkoxydim and pinoxaden, respectively, when compared with the susceptible Biotype. The levels of resistance and cross-resistance patterns varied among Biotypes indicating either more than one mechanism of resistance or different resistance mutations in these wild oat Biotypes. The CHD herbicides, sethoxydim and ...

  • identification of glyphosate resistant italian ryegrass lolium multiflorum in oregon
    Weed Science, 2005
    Co-Authors: Alejandro Perezjones, Carol A Mallorysmith, Kee Woong Park, Jed B Colquhoun, Dale L Shaner
    Abstract:

    Abstract A suspected glyphosate-resistant Italian ryegrass Biotype was collected from a filbert orchard near Portland, OR, where glyphosate was applied multiple times per year for about 15 yr. Greenhouse studies were conducted to determine if this Biotype was glyphosate resistant. The plants were sprayed with glyphosate (0.01 to 3.37 kg ae ha−1) 14 d after planting and shoot biomass was determined 3 wk after herbicide treatment. Based on the dose–response experiments conducted in the greenhouse, the suspected Italian ryegrass Biotype was approximately fivefold more resistant to glyphosate than the susceptible Biotype. Plants from both susceptible and resistant Biotypes were treated with glyphosate (0.42 and 0.84 kg ha−1) and shikimic acid was extracted 12, 24, 48, and 96 h after treatment. The susceptible Biotype accumulated between three and five times more shikimic acid than did the resistant Biotype. Leaf segments from both susceptible and resistant Biotypes were incubated with different glyphosate con...

  • absorption translocation and metabolism of propoxycarbazone sodium in als inhibitor resistant bromus tectorum Biotypes
    Pesticide Biochemistry and Physiology, 2004
    Co-Authors: Kee Woong Park, Lynn Fandrich, Carol A Mallorysmith
    Abstract:

    Abstract Experiments were conducted to investigate the absorption, translocation, and metabolism of propoxycarbazone-sodium in acetolactate synthase-inhibitor resistant (AR and MR) and susceptible (AS and MS) Bromus tectorum Biotypes. Absorption and translocation of l4 C-propoxycarbazone-sodium were similar in all Biotypes. One major and three minor metabolites were identified using reverse-phase high performance liquid chromatography. In all Biotypes, 80% of the propoxycarbazone-sodium was metabolized by 72 h after treatment (HAT). However, propoxycarbazone-sodium was metabolized more rapidly in the MR Biotype than in the other Biotypes. The half-life of propoxycarbazone-sodium in the MR Biotype was 8.9 h, which was 30, 36, and 40% shorter than in the AS, AR, and MS Biotypes, respectively. When 14 C-propoxycarbazone-sodium was applied with 1-aminobenzotriazole, a known cytochrome P450 inhibitor, metabolism decreased 20% 12 HAT in the MR Biotype. These results indicate that resistance of the MR Biotype to propoxycarbazone-sodium is due to a relatively rapid rate of propoxycarbazone-sodium metabolism compared to other B. tectorum Biotypes and that cytochrome P450s may be involved in the metabolism. The fact that these populations evolved so quickly and with different resistance mechanisms is a concern as more ALS inhibitors are introduced into the production systems.

  • physiological and molecular basis for als inhibitor resistance in bromus tectorum Biotypes
    Weed Research, 2004
    Co-Authors: K W Park, Carol A Mallorysmith
    Abstract:

    Summary Primisulfuron-resistant (AR and MR) and -susceptible (AS and MS) Bromus tectorum Biotypes were collected from a Poa pratensis field at Athena, Oregon, and in research plots at Madras, Oregon. Studies were conducted to characterize the resistance of the B. tectorum Biotypes. Whole plant bioassay and acetolactate synthase (ALS) enzyme assay revealed that the AR Biotype was highly resistant to the sulfonylurea (SU) herbicides, primisulfuron and sulfosulfuron and to a sulfonylaminocarbonyltriazolinone (SCT) herbicide, propoxycarbazone-sodium. However, the AR Biotype was not resistant to imazamox, an imidazolinone (IMI) herbicide. Results of the whole plant bioassay studies showed that the MR Biotype was moderately resistant to all ALS inhibitors tested. However, there were no differences in ALS sensitivities between the MR and MS Biotypes. The nucleotide and amino acid sequence analysis of the als gene demonstrated a single-point mutation from C to T, conferring the exchange of the amino acid proline to serine at position 197 in the AR Biotype. However, this mutation was not found in the MR Biotype. Results of this research indicate that: the resistance of the AR Biotype to SU and SCT herbicides is based on an altered target site due to a single-point mutation; resistance in the MR Biotype is not due to a target site mutation.

Jose Luis Cenis - One of the best experts on this subject based on the ideXlab platform.

  • Biotype q of bemisia tabaci identified in israel
    Phytoparasitica, 2003
    Co-Authors: A. R. Horowitz, Ian Denholm, Kevin Gorman, Jose Luis Cenis, Svetlana Kontsedalov, Isaac Ishaaya
    Abstract:

    The Biotype status of samples of the whiteflyBemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) collected from several field and greenhouse sites in Israel during 1999–2000 was determined by polyacrylamide gel electrophoresis (PAGE) for general esterases, and by RAPD-PCR using primers of arbitrary sequence. Results of this survey provide the first published evidence for the occurrence of theB. tabaci Q Biotype, alongside the more widely distributed B Biotype. Based on the collected samples, it appears that both the B and Q Biotypes are present in Israel, and that field populations consist of a mixture of the two Biotypes. A possible link betweenB. tabaci Biotypes and insecticide resistance is discussed.

  • genetic diversity of iberian populations of bemisia tabaci hemiptera aleyrodidae based on random amplified polymorphic dna polymerase chain reaction
    Molecular Ecology, 2001
    Co-Authors: Andres Moya, P Guirao, D Cifuentes, Francisco Beitia, Jose Luis Cenis
    Abstract:

    : The genetic structure of six Iberian populations of the whitefly Bemisia tabaci, two of them Biotype Q, one Biotype B, and the other three a mixture of both, has been studied using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). A total of 336 individuals was completely discriminated by means of 234 scored bands. Separate analyses of molecular variance of haploid males and diploid females using the pairwise number of differences between haplotypes showed that Biotypes contribute significantly more to the observed variability than populations within Biotypes. On average, gene flow between two Biotypes of the same population is lower than between populations of identical Biotypes. On the basis of these results and the nondetection under natural conditions of a single hybrid, we consider that both Biotypes are genetically isolated under the ecological conditions prevailing in the south Iberian Peninsula. All populations of Biotype Q presented similar values of intrapopulational diversity, which were higher than the values shown by populations of Biotype B.

  • genetic relationships among Biotypes of bemisia tabaci hemiptera aleyrodidae based on aflp analysis
    Bulletin of Entomological Research, 2000
    Co-Authors: Maria Teresa Cervera, Francisco Beitia, Jose Antonio Cabezas, B Simon, Jose M Martinezzapater, Jose Luis Cenis
    Abstract:

    Genetic similarities between 13 samples belonging to nine reference Biotypes and two field populations of Bemisia tabaci (Gennadius), one field population of B. medinae Gomez-Menor and another of B. afer Priesner & Hosny, were evaluated using amplified fragment length polymorphism (AFLP) markers. The results indicate that B. tabaci Biotypes can be grouped together with a minimum similarity coefficient of 0.32 and separated from the two other species with a similarity coefficient of 0.07. Bemisia tabaci Biotypes were grouped in four clusters which comprised: (i) Near East and Indian subcontinent Biotypes; (ii) B and Q Biotypes plus a Nigerian population from cowpea; (iii) New World A Biotype; and (iv) S Biotype and a Nigerian population from cassava. These results were consistent with a previous grouping of Biotypes based on RAPD-PCR analysis. The AFLP assay allowed the scoring of a total of 354 polymorphic bands in two reaction events with the use of two primer combinations.

Rafael De Prado - One of the best experts on this subject based on the ideXlab platform.

  • target site mutation and reduced translocation are present in a glyphosate resistant lolium multiflorum lam Biotype from spain
    Plant Physiology and Biochemistry, 2012
    Co-Authors: Fidel Gonzaleztorralva, Javier Gilhumanes, Francisco Barro, Ivo Brants, Rafael De Prado
    Abstract:

    Abstract The resistance mechanism of a glyphosate-resistant Lolium multiflorum Lam. Biotype collected in Cordoba (Southern Spain) was examined. Resistance Factor values at three different growth stages ranged between 4.77 and 4.91. At 96 hours after treatment (HAT) the S Biotype had accumulated seven times more shikimic acid than the R Biotype. There were significant differences in translocation of 14C-glyphosate between Biotypes, i.e. at 96 HAT, the R Biotype accumulated in the treated leaf more than 70% of the absorbed herbicide, in comparison with 59.21% of the S Biotype; the R Biotype translocated only 14.79% of the absorbed 14C-glyphosate to roots, while in the S population this value was 24.79%. Visualization of 14C-glyphosate by phosphor imaging showed a reduced distribution in the R Biotype compared with the S. Glyphosate metabolism was not involved in the resistance mechanism due to both Biotypes showing similar values of glyphosate at 96 HAT. Comparison of the EPSPS gene sequences between Biotypes indicated that the R Biotype has a proline 182 to serine amino acid substitution. In short, the resistance mechanism of the L. multiflorum Lam. Biotype is due to an impaired translocation of the herbicide and an altered target site.

  • pool of resistance mechanisms to glyphosate in digitaria insularis
    Journal of Agricultural and Food Chemistry, 2012
    Co-Authors: Leonardo Bianco De Carvalho, Fidel Gonzaleztorralva, Javier Gilhumanes, Francisco Barro, Rafael De Prado, Pedro Luis Da Costa Aguiar Alves, Hugo Cruzhipolito, Antonia M Rojanodelgado, Maria Dolores Luque De Castro
    Abstract:

    Digitaria insularis Biotypes resistant to glyphosate have been detected in Brazil. Studies were carried out in controlled conditions to determine the role of absorption, translocation, metabolism, and gene mutation as mechanisms of glyphosate resistance in D. insularis. The susceptible Biotype absorbed at least 12% more 14C-glyphosate up to 48 h after treatment (HAT) than resistant Biotypes. High differential 14C-glyphosate translocation was observed at 12 HAT, so that >70% of the absorbed herbicide remained in the treated leaf in resistant Biotypes, whereas 42% remained in the susceptible Biotype at 96 HAT. Glyphosate was degraded to aminomethylphosphonic acid (AMPA), glyoxylate, and sarcosine by >90% in resistant Biotypes, whereas a small amount of herbicide (up to 11%) was degraded by the susceptible Biotype up to 168 HAT. Two amino acid changes were found at positions 182 and 310 in EPSPS, consisting of a proline to threonine and a tyrosine to cysteine substitution, respectively, in resistant Biotypes...

  • mechanism of resistance to accase inhibiting herbicides in wild oat avena fatua from latin america
    Journal of Agricultural and Food Chemistry, 2011
    Co-Authors: Hugo Cruzhipolito, Maria D Osuna, Jose A Dominguezvalenzuela, N Espinoza, Rafael De Prado
    Abstract:

    Whole-plant response of two suspected resistant Avena fatua Biotypes from Chile and Mexico to ACCase-inhibiting herbicides [aryloxyphenoxypropionate (APP), cyclohexanedione (CHD), and pinoxaden (PPZ)] and the mechanism behind their resistance were studied. Both dose-response and ACCase enzyme activity assays revealed cross-resistance to the three herbicide families in the Biotype from Chile. On the other hand, the wild oat Biotype from Mexico exhibited resistance to the APP herbicides and cross-resistance to the CHD herbicides, but no resistance to PPZ. Differences in susceptibility between the two Biotypes were unrelated to absorption, translocation, and metabolism of the herbicides. PCR generated fragments of the ACCase CT domain spanning the potential mutations sited in the resistant and susceptible Biotypes were sequenced and compared. A point mutation was detected in the aspartic acid triplet at the amino acid position 2078 in the Chilean Biotype and in isoleucine at the amino acid position 2041 in the Mexican wild oat Biotype, which resulted in a glycine triplet and an asparagine triplet, respectively. On the basis of in vitro assays, the target enzyme (ACCase) in these resistant Biotypes contains a herbicide-insensitive form. This is the first reported evidence of resistance to pinoxaden in A. fatua.

Youjun Zhang - One of the best experts on this subject based on the ideXlab platform.

  • the suitability of Biotypes q and b of bemisia tabaci gennadius hemiptera aleyrodidae at different nymphal instars as hosts for encarsia formosa gahan hymenoptera aphelinidae
    PeerJ, 2016
    Co-Authors: Youjun Zhang, Qingjun Wu, Shaoli Wang
    Abstract:

    Encarsia formosa Gahan (Hymenoptera: Aphelinidae) is a solitary endoparasitoid that is commercially reared and released for augmentative biological control of whiteflies infesting greenhouse crops. In most areas in China, the invasive and destructive whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Biotype Q has replaced B. tabaci Biotype B and has become dominant between the two. A better understanding of the suitability of different nymphal instars of B. tabaci Biotypes Q and B as hosts for E. formosa is needed to improve the use of this parasitoid for biological control. Parasitism of the four nymphal instars of B. tabaci Biotypes Q and B by the commercial strain of E. formosa mass reared on Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae) was assessed in the laboratory. The results indicated that E. formosa parasitized and successfully developed on all instars of both Biotypes but performed best on the 3rd instar of B. tabaci Biotype B and on the 2nd, 3rd, and 4th instars of B. tabaci Biotype Q. The host-feeding rate of the adult parasitoid was generally higher on nymphal instars of B. tabaci Biotype Q than on the corresponding nymphal instars of Biotype B and was significantly higher on the 2nd and 3rd instars. For both whitefly Biotypes, the parasitoid’s immature developmental period was the longest on the 1st instar, intermediate on the 2nd and 3rd instars, and the shortest on the 4th instar. The parasitoid emergence rate was significantly lower on the 1st instar than on the other three instars and did not significantly differ between B. tabaci Biotype B and Biotype Q. Offspring longevity was greater on the 3rd and 4th instars than on the 1st instar and did not significantly differ between the two B. tabaci Biotypes. The results indicate that commercially-produced E. formosa can parasitize all instars of B. tabaci Biotypes B and Q, making this parasitoid a promising tool for the management of the two Biotypes of B. tabaci present in China.

  • further insights into the strange role of bacterial endosymbionts in whitefly bemisia tabaci comparison of secondary symbionts from Biotypes b and q in china
    Bulletin of Entomological Research, 2011
    Co-Authors: D Chu, Youjun Zhang, C S Gao, P J De Barro, F H Wan, I A Khan
    Abstract:

    The percentage infection of secondary symbionts (SS) (Wolbachia, Arsenophonus, Rickettsia, Hamiltonella, Fritschea and Cardinium) in the exotic Bemisia tabaci (Genn.) invaders, commonly known as Biotypes B and Q from China, were determined by PCR. In total, 373 Biotype B and 1830 Biotype Q individuals were screened for the presence of SS. Biotype B was more abundant than Biotype Q from 2005 to 2006, and Biotype Q was more abundant from 2007 to 2009. Each of the SS, with the exception of Fritschea, was detected in both Biotypes B and Q; Fritschea was found in none of the samples examined. For Biotype B, the percentage infection of Hamiltonella was the highest (92.0%) followed by Rickettsia (70.2%). For Biotype Q, the percentage infection of Hamiltonella was again the highest (73.3%). Arsenophonus was the least common of the SS observed in both Biotypes B and Q. The percentage infection of Wolbachia, Rickettsia and Hamiltonella in Biotype B was each significantly higher than in Biotype Q, whereas the percentage infection of Cardinium in Biotype B was significantly lower than in Biotype Q. The percentage infection of SS in Biotypes B and Q varied from year to year over the period 2005-2009. Furthermore, within Biotype Q, two distinct subgroups were identified which differ from each other in terms of their SS complement. We discuss these results in the light of the potentially influential factors and roles of the SS.

  • further spread of and domination by bemisia tabaci hemiptera aleyrodidae Biotype q on field crops in china
    Journal of Economic Entomology, 2011
    Co-Authors: Daqing Ge, Qingjun Wu, Shaoli Wang, Xiaoguo Jiao, Xin Yang, Nina Yang, Qi Su, Baoyun Xu, Youjun Zhang
    Abstract:

    The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), causes severe crop losses to many crops. The worst of these losses are often associated with the invasion and establishment of Biotypes B and Q of this pest. Previous research in 2007 showed that Biotype Q occurred with other Biotypes in most field populations in China. To determine the current status of the Biotype composition in the field, an extensive survey covering mainly eastern parts of China was conducted in 2009. Using polymerase chain reaction primers specific for the mitochondrial cytochrome oxidase I of Biotypes B and Q and gene sequencing, we determined the Biotypes composition in 61 whitefly populations and their distribution across 19 provinces in China. Our research revealed that only Biotypes B and Q have been found in the field in 2009 in China. Among them, Biotype Q was dominant in 44 locations (100.0%) and Biotype B was dominant in 17 locations (100.0%). The current survey indicates that Biotype Q has rapidly displaced Biotype B in most locations in China.

  • change in the Biotype composition of bemisia tabaci in shandong province of china from 2005 to 2008
    Environmental Entomology, 2010
    Co-Authors: Youjun Zhang, Judith K Brown
    Abstract:

    ABSTRACT Certain Biotypes of the Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) complex cause extensive damage and are important pests and virus vectors in agricultural crops throughout the world. Among the most invasive and well studied are the B and Q Biotypes. Recent reports in Shandong Province, China, have indicated that the Q Biotype was introduced there in ≈ 2005, whereas the B Biotype has been established there for ∼10 yr. Even so, the present distribution of the two Biotypes in Shandong has not been examined. The results of this study showed that the B and Q Biotypes are both present in Shandong Province based on bar-coding using a ≈450-base fragment of the mitochondrial cytochrome oxidase I (mtCOI) gene. In addition, a B Biotype-specific polymerase chain reaction primer pair that amplifies a ≈300 bp mtCOI fragment was designed and used to examine the Biotype composition of B. tabaci in selected crops from six provincial locations, using the general mtCOI primers as an internal positive cont...

  • invasive mechanism and management strategy of bemisia tabaci gennadius Biotype b progress report of 973 program on invasive alien species in china
    Science China-life Sciences, 2009
    Co-Authors: Fanghao Wan, Liansheng Zang, Youjun Zhang, Guifen Zhang, Shusheng Liu, Chen Luo, Dong Chu, Min Jiu, Xuhong Cui, Liping Zhang
    Abstract:

    Bemisia tabaci (Gennadius) Biotype B, called a “superbug”, is one of the most harmful Biotypes of this species complex worldwide. In this report, the invasive mechanism and management of B. tabaci Biotype B, based on our 5-year studies, are presented. Six B. tabaci Biotypes, B, Q, ZHJ1, ZHJ2, ZHJ3 and FJ1, have been identified in China. Biotype B dominates the other Biotypes in many regions of the country. Genetic diversity in Biotype B might be induced by host plant, geographical conditions, and/or insecticidal application. The activities of CarE (carboxylesterase) and GSTs (glutathione-S-transferase) in Biotype B reared on cucumber and squash were greater than on other host plants, which might have increased its resistance to insecticides. The higher activities of detoxification enzymes in Biotype B might be induced by the secondary metabolites in host plants. Higher adaptive ability of Biotype B adults to adverse conditions might be linked to the expression of heat shock protein genes. The indigenous B. tabaci Biotypes were displaced by the Biotype B within 225 d. The asymmetric mating interactions and mutualism between Biotype B and begomoviruses via its host plants speed up widespread invasion and displacement of other Biotypes. B. tabaci Biotype B displaced Trialeurodes vaporariorum (Westwood) after 4–7 generations under glasshouse conditions. Greater adaptive ability of the Biotype B to adverse conditions and its rapid population increase might be the reasons of its successful displacement of T. vaporariorum. Greater ability of the Biotype B to switch to different host plants may enrich its host plants, which might enable it to better compete with T. vaporariorum. Native predatory natural enemies possess greater ability to suppress B. tabaci under field conditions. The kairomones in the 3rd and 4th instars of Biotype B may provide an important stimulus in host searching and location by its parasitoids. The present results provide useful information in explaining the mechanisms of genetic diversity, evolution and molecular eco-adaptation of Biotype B. Furthermore, it provides a base for sustainable management of B. tabaci using biological and ecological measures.