The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Jay D Keasling - One of the best experts on this subject based on the ideXlab platform.
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carotenoid based phenotypic screen of the yeast deletion collection reveals new genes with roles in isoprenoid production
Metabolic Engineering, 2013Co-Authors: Bilge Ozaydin, Helcio Burd, Jay D KeaslingAbstract:Abstract Beside their essential cellular functions, isoprenoids have value as pharmaceuticals, nutriceuticals, pesticides, and fuel alternatives. Engineering microorganisms for production of isoprenoids is relatively easy, sustainable, and cost effective in comparison to chemical synthesis or extraction from natural producers. We introduced genes encoding carotenoid biosynthetic enzymes into the haploid yeast deletion collection to identify gene deletions that improved isoprenoid production. Deletions that showed significant improvement in carotenoid production were further screened for production of bisabolene, an isoprenoid alternative to petroleum-derived diesel. Combining those deletions with other mevalonate pathway modifications increased production of bisabolene from 40 mg/L to 800 mg/L in shake-flask cultures. In a fermentation process, this engineered strain produced 5.2 g/L of bisabolene.
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identification and microbial production of a terpene based advanced biofuel
Nature Communications, 2011Co-Authors: Pamela Peraltayahya, Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila MukhopadhyayAbstract:Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, Bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify Bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l(-1). We produce bisabolene in Saccharomyces cerevisiae (>900 mg l(-1)), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into Bisabolane. This work presents a framework for the identification of novel terpene-based advanced biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of biofuels.
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identification and microbial production of a terpene based advanced biofuel
Nature Communications, 2011Co-Authors: Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay, Pamela PeraltayahyaAbstract:Advanced biofuels with comparable properties to petroleum-based fuels could be microbially produced from lignocellulosic biomass. In this study, Escherichia coli is engineered to produce bisabolene, the immediate precursor of Bisabolane, a biosynthetic alternative to D2 diesel.
Armando Cordova - One of the best experts on this subject based on the ideXlab platform.
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catalytic enantioselective β alkylation of α β unsaturated aldehydes by combination of transition metal and aminocatalysis total synthesis of Bisabolane sesquiterpenes
ChemInform, 2011Co-Authors: Samson Afewerki, Palle Breistein, Luca Deiana, Pawel Dziedzic, Ismail Ibrahem, Kristian Pirttilae, Armando CordovaAbstract:The methodology is applied to the short total syntheses of Bisabolane sesquiterpenes (S)-(+)-curcumene, (E)-(S)-(+)-3-dehydrocurcumene and (S)-(+)-tumerone from product (IIIa).
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catalytic enantioselective β alkylation of α β unsaturated aldehydes by combination of transition metal and aminocatalysis total synthesis of Bisabolane sesquiterpenes
Chemistry: A European Journal, 2011Co-Authors: Samson Afewerki, Palle Breistein, Kristian Pirttila, Luca Deiana, Pawel Dziedzic, Ismail Ibrahem, Armando CordovaAbstract:Catalytic enantioselective β -alkylation of α,β-unsaturated aldehydes by combination of transition-metal- and aminocatalysis : Total synthesis of Bisabolane sesquiterpenes
Pamela Peraltayahya - One of the best experts on this subject based on the ideXlab platform.
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identification and microbial production of a terpene based advanced biofuel
Nature Communications, 2011Co-Authors: Pamela Peraltayahya, Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila MukhopadhyayAbstract:Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, Bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify Bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l(-1). We produce bisabolene in Saccharomyces cerevisiae (>900 mg l(-1)), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into Bisabolane. This work presents a framework for the identification of novel terpene-based advanced biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of biofuels.
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identification and microbial production of a terpene based advanced biofuel
Nature Communications, 2011Co-Authors: Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay, Pamela PeraltayahyaAbstract:Advanced biofuels with comparable properties to petroleum-based fuels could be microbially produced from lignocellulosic biomass. In this study, Escherichia coli is engineered to produce bisabolene, the immediate precursor of Bisabolane, a biosynthetic alternative to D2 diesel.
Vassilios Roussis - One of the best experts on this subject based on the ideXlab platform.
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Bisabolane and chamigrane sesquiterpenes from the soft coral pseudopterogorgia rigida
Phytochemistry Letters, 2014Co-Authors: Panagiota Georgantea, Constantinos Vagias, Efstathia Ioannou, Vassilios RoussisAbstract:A chemical investigation of the organic extract of the tropical soft coral Pseudopterogorgia rigida afforded 27 sesquiterpenes, among which one chamigrane (1) and seven Bisabolanes (2–8) are new natural products. The structures of compounds 1–8 were deduced by analysis of their NMR and MS data. The full assignment of the spectroscopic data of four aromatic Bisabolanes previously reported as semisynthetic products (11, 19, 22, 23) is reported.
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perezoperezone and curcuperezone Bisabolane dimers from the soft coral pseudopterogorgia rigida
Tetrahedron Letters, 2013Co-Authors: Panagiota Georgantea, Constantinos Vagias, Efstathia Ioannou, Vassilios RoussisAbstract:Perezoperezone (1), curcuperezone (2), and diperezone (3), belonging to the rare class of Bisabolane dimers, were isolated as minor constituents of the organic extract of the Caribbean soft coral, Pseudopterogorgia rigida. The structures of the new compounds 1 and 2 were established by detailed analyses of their NMR and MS data. 2013 Published by Elsevier Ltd.
Aindrila Mukhopadhyay - One of the best experts on this subject based on the ideXlab platform.
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identification and microbial production of a terpene based advanced biofuel
Nature Communications, 2011Co-Authors: Pamela Peraltayahya, Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila MukhopadhyayAbstract:Rising petroleum costs, trade imbalances and environmental concerns have stimulated efforts to advance the microbial production of fuels from lignocellulosic biomass. Here we identify a novel biosynthetic alternative to D2 diesel fuel, Bisabolane, and engineer microbial platforms for the production of its immediate precursor, bisabolene. First, we identify Bisabolane as an alternative to D2 diesel by measuring the fuel properties of chemically hydrogenated commercial bisabolene. Then, via a combination of enzyme screening and metabolic engineering, we obtain a more than tenfold increase in bisabolene titers in Escherichia coli to >900 mg l(-1). We produce bisabolene in Saccharomyces cerevisiae (>900 mg l(-1)), a widely used platform for the production of ethanol. Finally, we chemically hydrogenate biosynthetic bisabolene into Bisabolane. This work presents a framework for the identification of novel terpene-based advanced biofuels and the rapid engineering of microbial farnesyl diphosphate-overproducing platforms for the production of biofuels.
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identification and microbial production of a terpene based advanced biofuel
Nature Communications, 2011Co-Authors: Jay D Keasling, Mario Ouellet, Rossana Chan, Aindrila Mukhopadhyay, Pamela PeraltayahyaAbstract:Advanced biofuels with comparable properties to petroleum-based fuels could be microbially produced from lignocellulosic biomass. In this study, Escherichia coli is engineered to produce bisabolene, the immediate precursor of Bisabolane, a biosynthetic alternative to D2 diesel.