The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Úrsula Catalán - One of the best experts on this subject based on the ideXlab platform.
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Aqueous Stable Gold Nanostar/ZIF-8 Nanocomposites for Light-Triggered Release of Active Cargo Inside Living Cells.
Angewandte Chemie (International ed. in English), 2019Co-Authors: Carolina Carrillo-carrión, Raquel Martínez, María F. Navarro Poupard, Beatriz Pelaz, Ester Polo, Ana Arenas-vivo, Alessandro Olgiati, Pablo Taboada, Mahmoud Soliman, Úrsula CatalánAbstract:A plasmonic core-shell gold nanostar/zeolitic-imidazolate-framework-8 (ZIF-8) nanocomposite was developed for the thermoplasmonic-driven release of encapsulated active molecules inside living cells. The nanocomposites were loaded, as a proof of concept, with Bisbenzimide molecules as functional cargo and wrapped with an amphiphilic polymer that prevents ZIF-8 degradation and Bisbenzimide leaking in aqueous media or inside living cells. The demonstrated molecule-release mechanism relies on the use of near-IR light coupled to the plasmonic absorption of the core gold nanostars, which creates local temperature gradients and thus, Bisbenzimide thermodiffusion. Confocal microscopy and surface-enhanced Raman spectroscopy (SERS) were used to demonstrate Bisbenzimide loading/leaking and near-IR-triggered cargo release inside cells, thereby leading to DNA staining.
William F. Blakemore - One of the best experts on this subject based on the ideXlab platform.
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The effect of injection technique on the passive spread of astrocytes following transplantation into rat spinal cord white matter.
Restorative neurology and neuroscience, 1995Co-Authors: Natasha J. Olby, Marion T. O'leary, M. P. Targett, William F. BlakemoreAbstract:Bisbenzimide-labelled astrocytes were transplanted into the spinal white matter of the rat using three different injection techniques and the variability of the longitudinal distance over which they were found was compared 30 min later. Cells spread up to 5.02 mm and the greatest variability was seen when they were injected as a bolus (54%), compared with 26% when injected over 2 min. These results show the importance of establishing the extent of passive spread of cells and its variability when performing studies in cell migration.
Carolina Carrillo-carrión - One of the best experts on this subject based on the ideXlab platform.
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Aqueous Stable Gold Nanostar/ZIF-8 Nanocomposites for Light-Triggered Release of Active Cargo Inside Living Cells.
Angewandte Chemie (International ed. in English), 2019Co-Authors: Carolina Carrillo-carrión, Raquel Martínez, María F. Navarro Poupard, Beatriz Pelaz, Ester Polo, Ana Arenas-vivo, Alessandro Olgiati, Pablo Taboada, Mahmoud Soliman, Úrsula CatalánAbstract:A plasmonic core-shell gold nanostar/zeolitic-imidazolate-framework-8 (ZIF-8) nanocomposite was developed for the thermoplasmonic-driven release of encapsulated active molecules inside living cells. The nanocomposites were loaded, as a proof of concept, with Bisbenzimide molecules as functional cargo and wrapped with an amphiphilic polymer that prevents ZIF-8 degradation and Bisbenzimide leaking in aqueous media or inside living cells. The demonstrated molecule-release mechanism relies on the use of near-IR light coupled to the plasmonic absorption of the core gold nanostars, which creates local temperature gradients and thus, Bisbenzimide thermodiffusion. Confocal microscopy and surface-enhanced Raman spectroscopy (SERS) were used to demonstrate Bisbenzimide loading/leaking and near-IR-triggered cargo release inside cells, thereby leading to DNA staining.
Yoshikazu Nakai - One of the best experts on this subject based on the ideXlab platform.
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Enhanced Electron Transfer Reaction of Myoglobin in PEO by DNA/Bisbenzimide Complex
Polymer Journal, 1999Co-Authors: Hiroyuki Ohno, Yoshikazu NakaiAbstract:Double-stranded DNA was used as a molecular wire to enhance the electron transfer reaction between PEO-modified myoglobin (PEO-Mb) and electrode in PEO oligomers. No acceleration but suppression of electron transfer was found when only DNA was used. The π stacking of base-pairs in DNA was enhanced by the complexation with Bisbenzimide. Larger current of PEO-Mb was found under suitable values of the molar ratio of Bisbenzimide to DNA base pair, the quantity of cast PEO-Mb, and the ratio of DNA base pair to PEO-Mb. DNA/Bisbenzimide complex was revealed to be effective for the electron transfer reaction between heme-proteins and electrode even in ion conductive polymer matrix.
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enhanced electron transfer reaction of myoglobin in peo by dna Bisbenzimide complex
Polymer Journal, 1999Co-Authors: Hiroyuki Ohno, Yoshikazu NakaiAbstract:Double-stranded DNA was used as a molecular wire to enhance the electron transfer reaction between PEO-modified myoglobin (PEO-Mb) and electrode in PEO oligomers. No acceleration but suppression of electron transfer was found when only DNA was used. The π stacking of base-pairs in DNA was enhanced by the complexation with Bisbenzimide. Larger current of PEO-Mb was found under suitable values of the molar ratio of Bisbenzimide to DNA base pair, the quantity of cast PEO-Mb, and the ratio of DNA base pair to PEO-Mb. DNA/Bisbenzimide complex was revealed to be effective for the electron transfer reaction between heme-proteins and electrode even in ion conductive polymer matrix.
Kenzo Fujimoto - One of the best experts on this subject based on the ideXlab platform.
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Disassembly-driven signal turn-on probes for bimodal detection of DNA with 19F NMR and fluorescence.
Organic & biomolecular chemistry, 2018Co-Authors: Takashi Sakamoto, Daisaku Hasegawa, Kenzo FujimotoAbstract:To image DNAs in vivo, molecular probes that can detect DNA with a highly transparent signal are required. In this study, we designed and synthesized amphiphilic molecular probes that can detect DNA with a highly transparent 19F NMR signal in a signal turn-on manner based on the self-assembly and target-dependent-disassembly of the probe molecules in aqueous solution. Fluorescence and dynamic light scattering studies revealed that these amphiphilic probes consisting of Bisbenzimide H 33258 and 3,5-bis(trifluoromethyl)benzene are assembled and form aggregates in aqueous solution, and that the aggregates are collapsed when the probes bind to double-stranded DNA via selective interaction between the Bisbenzimide H 33258 moiety and AATT double-stranded region of DNA. Since the target-dependent-disassembly of the probes caused turn-on of 19F NMR and fluorescence signals, bimodal detection of double-stranded DNA was successfully achieved.
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Simultaneous detection of single-nucleotide polymorphisms in a DNA bulge structure using a fluorine-modified Bisbenzimide derivative
The Analyst, 2016Co-Authors: Takashi Sakamoto, Daisaku Hasegawa, Kenzo FujimotoAbstract:Using 3,5-bis(trifluoromethyl)benzene-modified Bisbenzimide H33258, bulged-out nucleobases on a DNA strand can be detected by the chemical shift change in (19)F NMR. Based on this technique, single-nucleotide polymorphisms on a DNA sequence can be analyzed simultaneously.
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Fluorine-modified Bisbenzimide derivative as a molecular probe for bimodal and simultaneous detection of DNAs by 19F NMR and fluorescence
Chemical communications (Cambridge England), 2015Co-Authors: Takashi Sakamoto, Daisaku Hasegawa, Kenzo FujimotoAbstract:3,5-Bis(trifluoromethyl)benzene modified Bisbenzimide H 33258 was synthesized as a 19F magnetic resonance-based DNA detection probe. The chemical shift and fluorescence of the probe were significantly changed by the addition of hairpin DNAs having an AATT sequence. The probe enables 19F NMR/fluorescence bimodal detection of the model DNA double strands simultaneously.