Black Swiss Mouse

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John C. Roder - One of the best experts on this subject based on the ideXlab platform.

  • NIH Swiss and Black Swiss mice have retinal degeneration and performance deficits in cognitive tests.
    Comparative Medicine, 2005
    Co-Authors: Steven J. Clapcote, Noah L. Lazar, Allison R. Bechard, Geoffrey A. Wood, John C. Roder
    Abstract:

    Swiss mice are among the most commonly used outbred strains in biomedical research. Because prior knowledge of the baseline phenotypes of Mouse strains will allow informed selection of strains for particular experiments, we sought to characterize the behavior of two previously untested outbred Swiss strains—NIH Swiss and Black Swiss—in the two most widely used paradigms for evaluating the cognitive abilities of mice. Unlike the C57BL/6J and C57BL/6J- Tyrc-2J controls, animals of both outbred Swiss strains were unable to demonstrate learning in the Morris water maze and contextual fear conditioning paradigms. A polymerase chain reaction assay revealed that all of the NIH Swiss and Black Swiss mice tested were homozygous for the recessive retinal degeneration 1 mutation of the Pde6b gene. Histological examination of NIH Swiss and Black Swiss Mouse eyes confi rmed the presence of retinal degeneration, which causes visual image blindness. These fi ndings indicate that NIH Swiss and Black Swiss mice are visually im- paired and thus may be unsuitable for use in some experiments.

Dane M. Chetkovich - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of HCN2 abnormalities as a cause of juvenile audiogenic seizures in Black Swiss mice
    Brain Research, 2006
    Co-Authors: Minyoung Shin, Dina Simkin, Genn Suyeoka, Dane M. Chetkovich
    Abstract:

    Abstract Epilepsy is an often-debilitating disease with many etiologies. Genetic predisposition is common for many of the generalized epilepsy syndromes, and mutations in genes encoding neuronal ion channels are causative in many cases. We previously identified a locus for juvenile audiogenic monogenic seizures (jams1) in the Black Swiss Mouse strain, delimited by the gene basigin (Bsg) and the marker D10Mit140. This region includes Hcn2, the gene encoding the hyperpolarization-activated cyclic nucleotide-gated channel subunit 2 (HCN2), an ion channel implicated in epilepsy. By sequencing genomic DNA, we found that Black Swiss mice have a single polymorphism in exon 2 within the Hcn2 gene. This single G/C to A/T base change alters the third position of a codon specifying alanine residue 293, without changing the predicted amino acid sequence. Furthermore, we found no detectable differences in HCN2 protein expression in the brains of Black Swiss mice, compared to control mice. We therefore reason that juvenile audiogenic seizures in Black Swiss mice are unlikely to be due to abnormalities of HCN2 channel function.

Steven J. Clapcote - One of the best experts on this subject based on the ideXlab platform.

  • NIH Swiss and Black Swiss mice have retinal degeneration and performance deficits in cognitive tests.
    Comparative Medicine, 2005
    Co-Authors: Steven J. Clapcote, Noah L. Lazar, Allison R. Bechard, Geoffrey A. Wood, John C. Roder
    Abstract:

    Swiss mice are among the most commonly used outbred strains in biomedical research. Because prior knowledge of the baseline phenotypes of Mouse strains will allow informed selection of strains for particular experiments, we sought to characterize the behavior of two previously untested outbred Swiss strains—NIH Swiss and Black Swiss—in the two most widely used paradigms for evaluating the cognitive abilities of mice. Unlike the C57BL/6J and C57BL/6J- Tyrc-2J controls, animals of both outbred Swiss strains were unable to demonstrate learning in the Morris water maze and contextual fear conditioning paradigms. A polymerase chain reaction assay revealed that all of the NIH Swiss and Black Swiss mice tested were homozygous for the recessive retinal degeneration 1 mutation of the Pde6b gene. Histological examination of NIH Swiss and Black Swiss Mouse eyes confi rmed the presence of retinal degeneration, which causes visual image blindness. These fi ndings indicate that NIH Swiss and Black Swiss mice are visually im- paired and thus may be unsuitable for use in some experiments.

Manuchehr Abedi-valugerdi - One of the best experts on this subject based on the ideXlab platform.

  • Mercury and silver induce B cell activation and anti-nucleolar autoantibody production in outbred Mouse stocks : are environmental factors more important than the susceptibility genes in connection with autoimmunity?
    Clinical and Experimental Immunology, 2009
    Co-Authors: Manuchehr Abedi-valugerdi
    Abstract:

    Environmental and predisposing genetic factors are known to play a crucial role in the development of systemic autoimmune diseases. With respect to the role of environmental factors, it is not known how and to what extent they contribute to the initiation and exacerbation of systemic autoimmunity. In the present study, I considered this issue and asked if environmental factors can induce autoimmunity in the absence of specific susceptible genes. The development of genetically controlled mercury- and silver-induced B cell activation and anti-nucleolar autoantibodies (ANolA) production in genetically heterozygous outbred Institute of Cancer Research (ICR), Naval Medical Research Institute (NMRI) and Black Swiss Mouse stocks were analysed. Four weeks of treatment with both mercury and silver induced a strong B cell activation characterized by increased numbers of splenic antibody-secreting cells of at least one or more immunoglobulin (Ig) isotype(s) in all treated stocks. The three stocks also exhibited a marked increase in the serum IgE levels in response to mercury, but not silver. More importantly, in response to mercury a large numbers of ICR (88%), NMRI (96%) and Black Swiss (100%) mice produced different levels of IgG1 and IgG2a ANolA (a characteristic which is linked strictly to the H-2 genes). Similarly, but at lower magnitudes, treatment with silver also induced the production of IgG1 and IgG2a ANolA in 60% of ICR, 75% of NMRI and 100% of Black Swiss mice. Thus, the findings of this study suggest that long-term exposure to certain environmental factors can activate the immune system to produce autoimmunity per se, without requiring specific susceptible genes.

Minyoung Shin - One of the best experts on this subject based on the ideXlab platform.

  • Evaluation of HCN2 abnormalities as a cause of juvenile audiogenic seizures in Black Swiss mice
    Brain Research, 2006
    Co-Authors: Minyoung Shin, Dina Simkin, Genn Suyeoka, Dane M. Chetkovich
    Abstract:

    Abstract Epilepsy is an often-debilitating disease with many etiologies. Genetic predisposition is common for many of the generalized epilepsy syndromes, and mutations in genes encoding neuronal ion channels are causative in many cases. We previously identified a locus for juvenile audiogenic monogenic seizures (jams1) in the Black Swiss Mouse strain, delimited by the gene basigin (Bsg) and the marker D10Mit140. This region includes Hcn2, the gene encoding the hyperpolarization-activated cyclic nucleotide-gated channel subunit 2 (HCN2), an ion channel implicated in epilepsy. By sequencing genomic DNA, we found that Black Swiss mice have a single polymorphism in exon 2 within the Hcn2 gene. This single G/C to A/T base change alters the third position of a codon specifying alanine residue 293, without changing the predicted amino acid sequence. Furthermore, we found no detectable differences in HCN2 protein expression in the brains of Black Swiss mice, compared to control mice. We therefore reason that juvenile audiogenic seizures in Black Swiss mice are unlikely to be due to abnormalities of HCN2 channel function.