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Black Swiss Mouse

The Experts below are selected from a list of 9 Experts worldwide ranked by ideXlab platform

John C. Roder – 1st expert on this subject based on the ideXlab platform

  • NIH Swiss and Black Swiss mice have retinal degeneration and performance deficits in cognitive tests.
    Comparative Medicine, 2005
    Co-Authors: Steven J. Clapcote, Noah L. Lazar, Allison R. Bechard, Geoffrey A. Wood, John C. Roder

    Abstract:

    Swiss mice are among the most commonly used outbred strains in biomedical research. Because prior knowledge of the baseline phenotypes of Mouse strains will allow informed selection of strains for particular experiments, we sought to characterize the behavior of two previously untested outbred Swiss strains—NIH Swiss and Black Swiss—in the two most widely used paradigms for evaluating the cognitive abilities of mice. Unlike the C57BL/6J and C57BL/6J- Tyrc-2J controls, animals of both outbred Swiss strains were unable to demonstrate learning in the Morris water maze and contextual fear conditioning paradigms. A polymerase chain reaction assay revealed that all of the NIH Swiss and Black Swiss mice tested were homozygous for the recessive retinal degeneration 1 mutation of the Pde6b gene. Histological examination of NIH Swiss and Black Swiss Mouse eyes confi rmed the presence of retinal degeneration, which causes visual image blindness. These fi ndings indicate that NIH Swiss and Black Swiss mice are visually im- paired and thus may be unsuitable for use in some experiments.

Dane M. Chetkovich – 2nd expert on this subject based on the ideXlab platform

  • Evaluation of HCN2 abnormalities as a cause of juvenile audiogenic seizures in Black Swiss mice
    Brain Research, 2006
    Co-Authors: Minyoung Shin, Dina Simkin, Genn Suyeoka, Dane M. Chetkovich

    Abstract:

    Abstract Epilepsy is an often-debilitating disease with many etiologies. Genetic predisposition is common for many of the generalized epilepsy syndromes, and mutations in genes encoding neuronal ion channels are causative in many cases. We previously identified a locus for juvenile audiogenic monogenic seizures (jams1) in the Black Swiss Mouse strain, delimited by the gene basigin (Bsg) and the marker D10Mit140. This region includes Hcn2, the gene encoding the hyperpolarization-activated cyclic nucleotide-gated channel subunit 2 (HCN2), an ion channel implicated in epilepsy. By sequencing genomic DNA, we found that Black Swiss mice have a single polymorphism in exon 2 within the Hcn2 gene. This single G/C to A/T base change alters the third position of a codon specifying alanine residue 293, without changing the predicted amino acid sequence. Furthermore, we found no detectable differences in HCN2 protein expression in the brains of Black Swiss mice, compared to control mice. We therefore reason that juvenile audiogenic seizures in Black Swiss mice are unlikely to be due to abnormalities of HCN2 channel function.

Steven J. Clapcote – 3rd expert on this subject based on the ideXlab platform

  • NIH Swiss and Black Swiss mice have retinal degeneration and performance deficits in cognitive tests.
    Comparative Medicine, 2005
    Co-Authors: Steven J. Clapcote, Noah L. Lazar, Allison R. Bechard, Geoffrey A. Wood, John C. Roder

    Abstract:

    Swiss mice are among the most commonly used outbred strains in biomedical research. Because prior knowledge of the baseline phenotypes of Mouse strains will allow informed selection of strains for particular experiments, we sought to characterize the behavior of two previously untested outbred Swiss strains—NIH Swiss and Black Swiss—in the two most widely used paradigms for evaluating the cognitive abilities of mice. Unlike the C57BL/6J and C57BL/6J- Tyrc-2J controls, animals of both outbred Swiss strains were unable to demonstrate learning in the Morris water maze and contextual fear conditioning paradigms. A polymerase chain reaction assay revealed that all of the NIH Swiss and Black Swiss mice tested were homozygous for the recessive retinal degeneration 1 mutation of the Pde6b gene. Histological examination of NIH Swiss and Black Swiss Mouse eyes confi rmed the presence of retinal degeneration, which causes visual image blindness. These fi ndings indicate that NIH Swiss and Black Swiss mice are visually im- paired and thus may be unsuitable for use in some experiments.