Developmental aspects of endothelin receptors in rabbit lower urinary tract.

Since evidence of development/age-related alterations of endothelin receptors in circulation and respiration systems has been increasing, we attempted to investigate the pharmacological characterization of endothelin receptors in neonatal, premature, and mature male rabbit lower urinary tract. The biochemical properties of ET receptors were examined in the lower urinary tracts of 1-day (neonatal)-, 6-week (premature)-, and 1-year(mature)-old male rabbits with binding technique utilizing [125I]ET-1.

The rank orders of the densities (Bmax values) of endothelin receptors in the Bladder Dome, Bladder base, and urethra of different aged rabbits were Bladder Dome, 1 day > 6 week vLs 1 year, Bladder base, 1 day > 6 week vLs 1 year, and urethra, 1 day > 6 week > 1 year. The pharmacological profiles of these binding sites inhibited by various kinds of endothelin receptor compounds showed similar Ki values and similar proportions of endothelin receptor subtypes in the same regions of 1-day-, 6-week-, and 1-year-old animals.

Our data clearly demonstrated the presence of regional difference and development-related changes in the density and subtype specificity of endothelin receptors in the lower urinary tract of the male rabbit. Neurourol. Urodynam. 19:71–85, 2000. © 2000 Wiley-Liss, Inc.

Age-related changes in contractile responses of rabbit lower urinary tract to endothelin.

Purpose: As there are significant amounts of endothelin (ET) receptors in the mammalian urinary tract, we investigated the pharmacological properties and localization of ET receptors in the rabbit lower urinary tract as a function of age.Materials and Methods: The characteristics of ET receptors in Bladder Dome, trigone and urethra of 6 weeks and 6 months old male rabbits were determined using muscle bath and autoradiographic techniques.Results: ET-1 produces significant contractile responses in smooth muscle strips from Bladder Dome, trigone, and urethra in both 6 weeks and 6 months old rabbits. Although there was no significant difference in the maximum contractile response of urethral muscle strips to ET-1 between 6 weeks and 6 months old rabbits, the maximum responses to ET-1 were higher in both Bladder Dome and trigone of 6 weeks than 6 months old rabbits. A selective ETA receptor antagonist, BQ 610, shifted the concentration response curve to ET-1 to the right without decreasing maximal contractile …

gene expression localization and pharmacological characterization of endothelin receptors in diabetic rat Bladder Dome

Abstract As there are significant amounts of functional endothelin receptors in the mammalian urinary tract, we examined the effect of experimental diabetes on the expression of endothelin receptors and their mRNAs in the rat Bladder Dome. The density of endothelin receptors in the rat Bladder Dome was higher (8 and 16 weeks following the onset of diabetes) than in age-matched controls. Insulin treatment, started 8 weeks after the induction of diabetes, partially reversed the endothelin receptor alterations. The pharmacological profile of the endothelin receptors in the Bladder Dome was similar in all groups and was consistent with the predominance of the endothelin ET A receptor subtype (ET A :ET B =approximately 4:1). Autoradiographic studies demonstrated that the endothelin receptors were located in all tissue components of the Bladder, including epithelial and muscular layers. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) data indicated that diabetes increased the expression level of gene transcripts for both endothelin receptor subtypes and that insulin treatment reversed the mRNA upregulation.

Developmental aspects of endothelin receptors in rabbit lower urinary tract.

Since evidence of development/age-related alterations of endothelin receptors in circulation and respiration systems has been increasing, we attempted to investigate the pharmacological characterization of endothelin receptors in neonatal, premature, and mature male rabbit lower urinary tract. The biochemical properties of ET receptors were examined in the lower urinary tracts of 1-day (neonatal)-, 6-week (premature)-, and 1-year(mature)-old male rabbits with binding technique utilizing [125I]ET-1.

The rank orders of the densities (Bmax values) of endothelin receptors in the Bladder Dome, Bladder base, and urethra of different aged rabbits were Bladder Dome, 1 day > 6 week vLs 1 year, Bladder base, 1 day > 6 week vLs 1 year, and urethra, 1 day > 6 week > 1 year. The pharmacological profiles of these binding sites inhibited by various kinds of endothelin receptor compounds showed similar Ki values and similar proportions of endothelin receptor subtypes in the same regions of 1-day-, 6-week-, and 1-year-old animals.

Our data clearly demonstrated the presence of regional difference and development-related changes in the density and subtype specificity of endothelin receptors in the lower urinary tract of the male rabbit. Neurourol. Urodynam. 19:71–85, 2000. © 2000 Wiley-Liss, Inc.

Age-related changes in contractile responses of rabbit lower urinary tract to endothelin.

Purpose: As there are significant amounts of endothelin (ET) receptors in the mammalian urinary tract, we investigated the pharmacological properties and localization of ET receptors in the rabbit lower urinary tract as a function of age.Materials and Methods: The characteristics of ET receptors in Bladder Dome, trigone and urethra of 6 weeks and 6 months old male rabbits were determined using muscle bath and autoradiographic techniques.Results: ET-1 produces significant contractile responses in smooth muscle strips from Bladder Dome, trigone, and urethra in both 6 weeks and 6 months old rabbits. Although there was no significant difference in the maximum contractile response of urethral muscle strips to ET-1 between 6 weeks and 6 months old rabbits, the maximum responses to ET-1 were higher in both Bladder Dome and trigone of 6 weeks than 6 months old rabbits. A selective ETA receptor antagonist, BQ 610, shifted the concentration response curve to ET-1 to the right without decreasing maximal contractile …

gene expression localization and pharmacological characterization of endothelin receptors in diabetic rat Bladder Dome

Abstract As there are significant amounts of functional endothelin receptors in the mammalian urinary tract, we examined the effect of experimental diabetes on the expression of endothelin receptors and their mRNAs in the rat Bladder Dome. The density of endothelin receptors in the rat Bladder Dome was higher (8 and 16 weeks following the onset of diabetes) than in age-matched controls. Insulin treatment, started 8 weeks after the induction of diabetes, partially reversed the endothelin receptor alterations. The pharmacological profile of the endothelin receptors in the Bladder Dome was similar in all groups and was consistent with the predominance of the endothelin ET A receptor subtype (ET A :ET B =approximately 4:1). Autoradiographic studies demonstrated that the endothelin receptors were located in all tissue components of the Bladder, including epithelial and muscular layers. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) data indicated that diabetes increased the expression level of gene transcripts for both endothelin receptor subtypes and that insulin treatment reversed the mRNA upregulation.

Developmental aspects of endothelin receptors in rabbit lower urinary tract.

Since evidence of development/age-related alterations of endothelin receptors in circulation and respiration systems has been increasing, we attempted to investigate the pharmacological characterization of endothelin receptors in neonatal, premature, and mature male rabbit lower urinary tract. The biochemical properties of ET receptors were examined in the lower urinary tracts of 1-day (neonatal)-, 6-week (premature)-, and 1-year(mature)-old male rabbits with binding technique utilizing [125I]ET-1.

The rank orders of the densities (Bmax values) of endothelin receptors in the Bladder Dome, Bladder base, and urethra of different aged rabbits were Bladder Dome, 1 day > 6 week vLs 1 year, Bladder base, 1 day > 6 week vLs 1 year, and urethra, 1 day > 6 week > 1 year. The pharmacological profiles of these binding sites inhibited by various kinds of endothelin receptor compounds showed similar Ki values and similar proportions of endothelin receptor subtypes in the same regions of 1-day-, 6-week-, and 1-year-old animals.

Our data clearly demonstrated the presence of regional difference and development-related changes in the density and subtype specificity of endothelin receptors in the lower urinary tract of the male rabbit. Neurourol. Urodynam. 19:71–85, 2000. © 2000 Wiley-Liss, Inc.

Age-related changes in contractile responses of rabbit lower urinary tract to endothelin.

Purpose: As there are significant amounts of endothelin (ET) receptors in the mammalian urinary tract, we investigated the pharmacological properties and localization of ET receptors in the rabbit lower urinary tract as a function of age.Materials and Methods: The characteristics of ET receptors in Bladder Dome, trigone and urethra of 6 weeks and 6 months old male rabbits were determined using muscle bath and autoradiographic techniques.Results: ET-1 produces significant contractile responses in smooth muscle strips from Bladder Dome, trigone, and urethra in both 6 weeks and 6 months old rabbits. Although there was no significant difference in the maximum contractile response of urethral muscle strips to ET-1 between 6 weeks and 6 months old rabbits, the maximum responses to ET-1 were higher in both Bladder Dome and trigone of 6 weeks than 6 months old rabbits. A selective ETA receptor antagonist, BQ 610, shifted the concentration response curve to ET-1 to the right without decreasing maximal contractile …

gene expression localization and pharmacological characterization of endothelin receptors in diabetic rat Bladder Dome

Abstract As there are significant amounts of functional endothelin receptors in the mammalian urinary tract, we examined the effect of experimental diabetes on the expression of endothelin receptors and their mRNAs in the rat Bladder Dome. The density of endothelin receptors in the rat Bladder Dome was higher (8 and 16 weeks following the onset of diabetes) than in age-matched controls. Insulin treatment, started 8 weeks after the induction of diabetes, partially reversed the endothelin receptor alterations. The pharmacological profile of the endothelin receptors in the Bladder Dome was similar in all groups and was consistent with the predominance of the endothelin ET A receptor subtype (ET A :ET B =approximately 4:1). Autoradiographic studies demonstrated that the endothelin receptors were located in all tissue components of the Bladder, including epithelial and muscular layers. Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) data indicated that diabetes increased the expression level of gene transcripts for both endothelin receptor subtypes and that insulin treatment reversed the mRNA upregulation.