The Experts below are selected from a list of 3201 Experts worldwide ranked by ideXlab platform
Kang Sun - One of the best experts on this subject based on the ideXlab platform.
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trilayer three dimensional hydrogel composite scaffold containing encapsulated adipose derived stem cells promotes Bladder Reconstruction via sdf 1α cxcr4 pathway
ACS Applied Materials & Interfaces, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4, and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a trilayer “sandwich” scaffold with minimal interference in systemic homeostasis. ASCs in BAMG-HS promoted morphological and histological Bladder restoration by accelerating scaffold degradation (p < 0.05), ameliorating fibrosis (p < 0.05) and inflammation (p < 0.01). Additionally, ASCs facilitated the recovery of Bladder function by enhancing smooth muscle regeneration (p < 0.05), innervation (p < 0.01) and angiogenesis (p < 0.001). Except for a small number of endothelium-differentiated ASCs, the pro-...
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adipose derived stem cells seeded Bladder acellular matrix graft silk fibroin enhances Bladder Reconstruction in a rat model
Oncotarget, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:// Dongdong Xiao 1, * , Qiong Wang 2, * , Hao Yan 1 , Xiangguo Lv 1 , Yang Zhao 1 , Zhe Zhou 1 , Ming Zhang 1 , Qian Sun 3 , Kang Sun 3 , Wei Li 3, ** and Mujun Lu 1, ** 1 Department of Urology and Andrology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China 2 Department of Urology, The Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China 3 The State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China * These authors contributed equally and are co-first authors of this manuscript ** These authors are co-corresponding authors of this manuscript Correspondence to: Mujun Lu, email: lumujun@163.com Wei Li, email: liwei1016_2000@sjtu.edu.cn Keywords: Bladder augmentation, adipose stem cell, silk fibroin, Bladder acellular matrix graft, angiogenesis Received: July 22, 2017 Accepted: August 28, 2017 Published: September 23, 2017 ABSTRACT The unfavourable clinical outcomes of host cell-seeded scaffolds for Bladder augmentation warrant improved bioactive biomaterials. This study aimed to examine the feasibility of adipose-derived stem cells (ASCs)-seeded bilayer Bladder acellular matrix graft (BAMG)-silk fibroin (SF) scaffold in enhancing Bladder Reconstruction. Sprague Dawley rats were randomly divided into three groups: the BAMG-SF-ASCs group, the acellular BAMG-SF group and the cystotomy group. The BAMG-SF-ASCs group was sampled at 2, 4 and 12 weeks, and compared with the other groups at 12 weeks. In the BAMG-SF-ASCs group, the normal Bladder contour was reformed similar to that in the cystotomy group, with abundant urothelium and smooth muscle regeneration, as well as a suitable scaffold degradation speed, and trivial fibrosis and inflammation. The ASCs seeded in BAMG-SF were maintained in the regenerated region during the 12-week experimental period and significantly enhanced the vessel density, nerve regeneration and Bladder function compared with acellular BAMG-SF. In addition, the BAMG-SF-ASCs group presented elevated levels of SDF-1α, VEGF and their receptors, with an obvious increase in ERK 1/2 phosphorylation. BAMG-SF is a promising biomaterial for ASCs seeding to facilitate Bladder augmentation and demonstrated an enhanced angiogenic potential possibly related to the SDF-1α/CXCR4 pathway via ERK 1/2 activation.
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Trilayer Three-Dimensional Hydrogel Composite Scaffold Containing Encapsulated Adipose-Derived Stem Cells Promotes Bladder Reconstruction via SDF-1α/CXCR4 Pathway
2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4, and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a trilayer “sandwich” scaffold with minimal interference in systemic homeostasis. ASCs in BAMG-HS promoted morphological and histological Bladder restoration by accelerating scaffold degradation (p < 0.05), ameliorating fibrosis (p < 0.05) and inflammation (p < 0.01). Additionally, ASCs facilitated the recovery of Bladder function by enhancing smooth muscle regeneration (p < 0.05), innervation (p < 0.01) and angiogenesis (p < 0.001). Except for a small number of endothelium-differentiated ASCs, the pro-angiogenic effects of ASCs were mainly related to ERK1/2 phosphorylation in the downstream of SDF-1α/CXCR4 pathway
Dongdong Xiao - One of the best experts on this subject based on the ideXlab platform.
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trilayer three dimensional hydrogel composite scaffold containing encapsulated adipose derived stem cells promotes Bladder Reconstruction via sdf 1α cxcr4 pathway
ACS Applied Materials & Interfaces, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4, and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a trilayer “sandwich” scaffold with minimal interference in systemic homeostasis. ASCs in BAMG-HS promoted morphological and histological Bladder restoration by accelerating scaffold degradation (p < 0.05), ameliorating fibrosis (p < 0.05) and inflammation (p < 0.01). Additionally, ASCs facilitated the recovery of Bladder function by enhancing smooth muscle regeneration (p < 0.05), innervation (p < 0.01) and angiogenesis (p < 0.001). Except for a small number of endothelium-differentiated ASCs, the pro-...
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adipose derived stem cells seeded Bladder acellular matrix graft silk fibroin enhances Bladder Reconstruction in a rat model
Oncotarget, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:// Dongdong Xiao 1, * , Qiong Wang 2, * , Hao Yan 1 , Xiangguo Lv 1 , Yang Zhao 1 , Zhe Zhou 1 , Ming Zhang 1 , Qian Sun 3 , Kang Sun 3 , Wei Li 3, ** and Mujun Lu 1, ** 1 Department of Urology and Andrology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China 2 Department of Urology, The Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China 3 The State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China * These authors contributed equally and are co-first authors of this manuscript ** These authors are co-corresponding authors of this manuscript Correspondence to: Mujun Lu, email: lumujun@163.com Wei Li, email: liwei1016_2000@sjtu.edu.cn Keywords: Bladder augmentation, adipose stem cell, silk fibroin, Bladder acellular matrix graft, angiogenesis Received: July 22, 2017 Accepted: August 28, 2017 Published: September 23, 2017 ABSTRACT The unfavourable clinical outcomes of host cell-seeded scaffolds for Bladder augmentation warrant improved bioactive biomaterials. This study aimed to examine the feasibility of adipose-derived stem cells (ASCs)-seeded bilayer Bladder acellular matrix graft (BAMG)-silk fibroin (SF) scaffold in enhancing Bladder Reconstruction. Sprague Dawley rats were randomly divided into three groups: the BAMG-SF-ASCs group, the acellular BAMG-SF group and the cystotomy group. The BAMG-SF-ASCs group was sampled at 2, 4 and 12 weeks, and compared with the other groups at 12 weeks. In the BAMG-SF-ASCs group, the normal Bladder contour was reformed similar to that in the cystotomy group, with abundant urothelium and smooth muscle regeneration, as well as a suitable scaffold degradation speed, and trivial fibrosis and inflammation. The ASCs seeded in BAMG-SF were maintained in the regenerated region during the 12-week experimental period and significantly enhanced the vessel density, nerve regeneration and Bladder function compared with acellular BAMG-SF. In addition, the BAMG-SF-ASCs group presented elevated levels of SDF-1α, VEGF and their receptors, with an obvious increase in ERK 1/2 phosphorylation. BAMG-SF is a promising biomaterial for ASCs seeding to facilitate Bladder augmentation and demonstrated an enhanced angiogenic potential possibly related to the SDF-1α/CXCR4 pathway via ERK 1/2 activation.
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comparison of morphological and functional restoration between asymmetric bilayer chitosan and Bladder acellular matrix graft for Bladder augmentation in a rat model
RSC Advances, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, An Qin, Yang Zhao, Ming Zhang, Zhe ZhouAbstract:Because of the limitations of current scaffolds and unfavorable results of clinical trials, proper scaffolds facilitating Bladder Reconstruction are highly desirable. The aim of this study was to evaluate a novel asymmetric bilayer chitosan scaffold compared with conventional Bladder acellular matrix graft (BAMG) in a rat model of Bladder augmentation. Twenty-four 8 week-old male Sprague-Dawley rats were randomly assigned to the chitosan scaffold, BAMG and cystotomy groups. The rats' Bladders were sampled for cystography and routine histological examination at 21 and 70 days. Immunofluorescence photometry, conscious cystometry, quantitative real-time polymerase chain reaction, and western blot analyses were performed using Bladders at 70 days. Compared with BAMG, the chitosan scaffold consisted of a membrane-like compact layer and a sponge-like porous layer with an excellent combination of mechanical strength and flexibility. The chitosan group showed better performances than the BAMG group in radiographic cystography, smooth muscle regeneration, blood vessel numbers and functional restoration. In contrast to reduced Bladder compliance induced by BAMG, Bladder augmented by chitosan displayed nearly 1.5-fold increased Bladder capacity with comparable compliance to that of the cystotomy group at 70 days. The chitosan group exhibited higher levels of VEGF and VEGFR2, associated with the activation of the hypoxia-related SDF-1α/CXCR4 pathway. These results suggested that the asymmetric bilayer chitosan scaffold is a promising scaffold for Bladder Reconstruction.
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Trilayer Three-Dimensional Hydrogel Composite Scaffold Containing Encapsulated Adipose-Derived Stem Cells Promotes Bladder Reconstruction via SDF-1α/CXCR4 Pathway
2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4, and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a trilayer “sandwich” scaffold with minimal interference in systemic homeostasis. ASCs in BAMG-HS promoted morphological and histological Bladder restoration by accelerating scaffold degradation (p < 0.05), ameliorating fibrosis (p < 0.05) and inflammation (p < 0.01). Additionally, ASCs facilitated the recovery of Bladder function by enhancing smooth muscle regeneration (p < 0.05), innervation (p < 0.01) and angiogenesis (p < 0.001). Except for a small number of endothelium-differentiated ASCs, the pro-angiogenic effects of ASCs were mainly related to ERK1/2 phosphorylation in the downstream of SDF-1α/CXCR4 pathway
Zhe Zhou - One of the best experts on this subject based on the ideXlab platform.
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trilayer three dimensional hydrogel composite scaffold containing encapsulated adipose derived stem cells promotes Bladder Reconstruction via sdf 1α cxcr4 pathway
ACS Applied Materials & Interfaces, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4, and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a trilayer “sandwich” scaffold with minimal interference in systemic homeostasis. ASCs in BAMG-HS promoted morphological and histological Bladder restoration by accelerating scaffold degradation (p < 0.05), ameliorating fibrosis (p < 0.05) and inflammation (p < 0.01). Additionally, ASCs facilitated the recovery of Bladder function by enhancing smooth muscle regeneration (p < 0.05), innervation (p < 0.01) and angiogenesis (p < 0.001). Except for a small number of endothelium-differentiated ASCs, the pro-...
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adipose derived stem cells seeded Bladder acellular matrix graft silk fibroin enhances Bladder Reconstruction in a rat model
Oncotarget, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:// Dongdong Xiao 1, * , Qiong Wang 2, * , Hao Yan 1 , Xiangguo Lv 1 , Yang Zhao 1 , Zhe Zhou 1 , Ming Zhang 1 , Qian Sun 3 , Kang Sun 3 , Wei Li 3, ** and Mujun Lu 1, ** 1 Department of Urology and Andrology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China 2 Department of Urology, The Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China 3 The State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China * These authors contributed equally and are co-first authors of this manuscript ** These authors are co-corresponding authors of this manuscript Correspondence to: Mujun Lu, email: lumujun@163.com Wei Li, email: liwei1016_2000@sjtu.edu.cn Keywords: Bladder augmentation, adipose stem cell, silk fibroin, Bladder acellular matrix graft, angiogenesis Received: July 22, 2017 Accepted: August 28, 2017 Published: September 23, 2017 ABSTRACT The unfavourable clinical outcomes of host cell-seeded scaffolds for Bladder augmentation warrant improved bioactive biomaterials. This study aimed to examine the feasibility of adipose-derived stem cells (ASCs)-seeded bilayer Bladder acellular matrix graft (BAMG)-silk fibroin (SF) scaffold in enhancing Bladder Reconstruction. Sprague Dawley rats were randomly divided into three groups: the BAMG-SF-ASCs group, the acellular BAMG-SF group and the cystotomy group. The BAMG-SF-ASCs group was sampled at 2, 4 and 12 weeks, and compared with the other groups at 12 weeks. In the BAMG-SF-ASCs group, the normal Bladder contour was reformed similar to that in the cystotomy group, with abundant urothelium and smooth muscle regeneration, as well as a suitable scaffold degradation speed, and trivial fibrosis and inflammation. The ASCs seeded in BAMG-SF were maintained in the regenerated region during the 12-week experimental period and significantly enhanced the vessel density, nerve regeneration and Bladder function compared with acellular BAMG-SF. In addition, the BAMG-SF-ASCs group presented elevated levels of SDF-1α, VEGF and their receptors, with an obvious increase in ERK 1/2 phosphorylation. BAMG-SF is a promising biomaterial for ASCs seeding to facilitate Bladder augmentation and demonstrated an enhanced angiogenic potential possibly related to the SDF-1α/CXCR4 pathway via ERK 1/2 activation.
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comparison of morphological and functional restoration between asymmetric bilayer chitosan and Bladder acellular matrix graft for Bladder augmentation in a rat model
RSC Advances, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, An Qin, Yang Zhao, Ming Zhang, Zhe ZhouAbstract:Because of the limitations of current scaffolds and unfavorable results of clinical trials, proper scaffolds facilitating Bladder Reconstruction are highly desirable. The aim of this study was to evaluate a novel asymmetric bilayer chitosan scaffold compared with conventional Bladder acellular matrix graft (BAMG) in a rat model of Bladder augmentation. Twenty-four 8 week-old male Sprague-Dawley rats were randomly assigned to the chitosan scaffold, BAMG and cystotomy groups. The rats' Bladders were sampled for cystography and routine histological examination at 21 and 70 days. Immunofluorescence photometry, conscious cystometry, quantitative real-time polymerase chain reaction, and western blot analyses were performed using Bladders at 70 days. Compared with BAMG, the chitosan scaffold consisted of a membrane-like compact layer and a sponge-like porous layer with an excellent combination of mechanical strength and flexibility. The chitosan group showed better performances than the BAMG group in radiographic cystography, smooth muscle regeneration, blood vessel numbers and functional restoration. In contrast to reduced Bladder compliance induced by BAMG, Bladder augmented by chitosan displayed nearly 1.5-fold increased Bladder capacity with comparable compliance to that of the cystotomy group at 70 days. The chitosan group exhibited higher levels of VEGF and VEGFR2, associated with the activation of the hypoxia-related SDF-1α/CXCR4 pathway. These results suggested that the asymmetric bilayer chitosan scaffold is a promising scaffold for Bladder Reconstruction.
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Trilayer Three-Dimensional Hydrogel Composite Scaffold Containing Encapsulated Adipose-Derived Stem Cells Promotes Bladder Reconstruction via SDF-1α/CXCR4 Pathway
2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4, and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a trilayer “sandwich” scaffold with minimal interference in systemic homeostasis. ASCs in BAMG-HS promoted morphological and histological Bladder restoration by accelerating scaffold degradation (p < 0.05), ameliorating fibrosis (p < 0.05) and inflammation (p < 0.01). Additionally, ASCs facilitated the recovery of Bladder function by enhancing smooth muscle regeneration (p < 0.05), innervation (p < 0.01) and angiogenesis (p < 0.001). Except for a small number of endothelium-differentiated ASCs, the pro-angiogenic effects of ASCs were mainly related to ERK1/2 phosphorylation in the downstream of SDF-1α/CXCR4 pathway
Hao Yan - One of the best experts on this subject based on the ideXlab platform.
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trilayer three dimensional hydrogel composite scaffold containing encapsulated adipose derived stem cells promotes Bladder Reconstruction via sdf 1α cxcr4 pathway
ACS Applied Materials & Interfaces, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4, and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a trilayer “sandwich” scaffold with minimal interference in systemic homeostasis. ASCs in BAMG-HS promoted morphological and histological Bladder restoration by accelerating scaffold degradation (p < 0.05), ameliorating fibrosis (p < 0.05) and inflammation (p < 0.01). Additionally, ASCs facilitated the recovery of Bladder function by enhancing smooth muscle regeneration (p < 0.05), innervation (p < 0.01) and angiogenesis (p < 0.001). Except for a small number of endothelium-differentiated ASCs, the pro-...
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adipose derived stem cells seeded Bladder acellular matrix graft silk fibroin enhances Bladder Reconstruction in a rat model
Oncotarget, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:// Dongdong Xiao 1, * , Qiong Wang 2, * , Hao Yan 1 , Xiangguo Lv 1 , Yang Zhao 1 , Zhe Zhou 1 , Ming Zhang 1 , Qian Sun 3 , Kang Sun 3 , Wei Li 3, ** and Mujun Lu 1, ** 1 Department of Urology and Andrology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China 2 Department of Urology, The Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China 3 The State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China * These authors contributed equally and are co-first authors of this manuscript ** These authors are co-corresponding authors of this manuscript Correspondence to: Mujun Lu, email: lumujun@163.com Wei Li, email: liwei1016_2000@sjtu.edu.cn Keywords: Bladder augmentation, adipose stem cell, silk fibroin, Bladder acellular matrix graft, angiogenesis Received: July 22, 2017 Accepted: August 28, 2017 Published: September 23, 2017 ABSTRACT The unfavourable clinical outcomes of host cell-seeded scaffolds for Bladder augmentation warrant improved bioactive biomaterials. This study aimed to examine the feasibility of adipose-derived stem cells (ASCs)-seeded bilayer Bladder acellular matrix graft (BAMG)-silk fibroin (SF) scaffold in enhancing Bladder Reconstruction. Sprague Dawley rats were randomly divided into three groups: the BAMG-SF-ASCs group, the acellular BAMG-SF group and the cystotomy group. The BAMG-SF-ASCs group was sampled at 2, 4 and 12 weeks, and compared with the other groups at 12 weeks. In the BAMG-SF-ASCs group, the normal Bladder contour was reformed similar to that in the cystotomy group, with abundant urothelium and smooth muscle regeneration, as well as a suitable scaffold degradation speed, and trivial fibrosis and inflammation. The ASCs seeded in BAMG-SF were maintained in the regenerated region during the 12-week experimental period and significantly enhanced the vessel density, nerve regeneration and Bladder function compared with acellular BAMG-SF. In addition, the BAMG-SF-ASCs group presented elevated levels of SDF-1α, VEGF and their receptors, with an obvious increase in ERK 1/2 phosphorylation. BAMG-SF is a promising biomaterial for ASCs seeding to facilitate Bladder augmentation and demonstrated an enhanced angiogenic potential possibly related to the SDF-1α/CXCR4 pathway via ERK 1/2 activation.
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comparison of morphological and functional restoration between asymmetric bilayer chitosan and Bladder acellular matrix graft for Bladder augmentation in a rat model
RSC Advances, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, An Qin, Yang Zhao, Ming Zhang, Zhe ZhouAbstract:Because of the limitations of current scaffolds and unfavorable results of clinical trials, proper scaffolds facilitating Bladder Reconstruction are highly desirable. The aim of this study was to evaluate a novel asymmetric bilayer chitosan scaffold compared with conventional Bladder acellular matrix graft (BAMG) in a rat model of Bladder augmentation. Twenty-four 8 week-old male Sprague-Dawley rats were randomly assigned to the chitosan scaffold, BAMG and cystotomy groups. The rats' Bladders were sampled for cystography and routine histological examination at 21 and 70 days. Immunofluorescence photometry, conscious cystometry, quantitative real-time polymerase chain reaction, and western blot analyses were performed using Bladders at 70 days. Compared with BAMG, the chitosan scaffold consisted of a membrane-like compact layer and a sponge-like porous layer with an excellent combination of mechanical strength and flexibility. The chitosan group showed better performances than the BAMG group in radiographic cystography, smooth muscle regeneration, blood vessel numbers and functional restoration. In contrast to reduced Bladder compliance induced by BAMG, Bladder augmented by chitosan displayed nearly 1.5-fold increased Bladder capacity with comparable compliance to that of the cystotomy group at 70 days. The chitosan group exhibited higher levels of VEGF and VEGFR2, associated with the activation of the hypoxia-related SDF-1α/CXCR4 pathway. These results suggested that the asymmetric bilayer chitosan scaffold is a promising scaffold for Bladder Reconstruction.
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Trilayer Three-Dimensional Hydrogel Composite Scaffold Containing Encapsulated Adipose-Derived Stem Cells Promotes Bladder Reconstruction via SDF-1α/CXCR4 Pathway
2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4, and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a trilayer “sandwich” scaffold with minimal interference in systemic homeostasis. ASCs in BAMG-HS promoted morphological and histological Bladder restoration by accelerating scaffold degradation (p < 0.05), ameliorating fibrosis (p < 0.05) and inflammation (p < 0.01). Additionally, ASCs facilitated the recovery of Bladder function by enhancing smooth muscle regeneration (p < 0.05), innervation (p < 0.01) and angiogenesis (p < 0.001). Except for a small number of endothelium-differentiated ASCs, the pro-angiogenic effects of ASCs were mainly related to ERK1/2 phosphorylation in the downstream of SDF-1α/CXCR4 pathway
Yang Zhao - One of the best experts on this subject based on the ideXlab platform.
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trilayer three dimensional hydrogel composite scaffold containing encapsulated adipose derived stem cells promotes Bladder Reconstruction via sdf 1α cxcr4 pathway
ACS Applied Materials & Interfaces, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4, and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a trilayer “sandwich” scaffold with minimal interference in systemic homeostasis. ASCs in BAMG-HS promoted morphological and histological Bladder restoration by accelerating scaffold degradation (p < 0.05), ameliorating fibrosis (p < 0.05) and inflammation (p < 0.01). Additionally, ASCs facilitated the recovery of Bladder function by enhancing smooth muscle regeneration (p < 0.05), innervation (p < 0.01) and angiogenesis (p < 0.001). Except for a small number of endothelium-differentiated ASCs, the pro-...
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adipose derived stem cells seeded Bladder acellular matrix graft silk fibroin enhances Bladder Reconstruction in a rat model
Oncotarget, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:// Dongdong Xiao 1, * , Qiong Wang 2, * , Hao Yan 1 , Xiangguo Lv 1 , Yang Zhao 1 , Zhe Zhou 1 , Ming Zhang 1 , Qian Sun 3 , Kang Sun 3 , Wei Li 3, ** and Mujun Lu 1, ** 1 Department of Urology and Andrology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200001, China 2 Department of Urology, The Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China 3 The State Key Lab of Metal Matrix Composites, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China * These authors contributed equally and are co-first authors of this manuscript ** These authors are co-corresponding authors of this manuscript Correspondence to: Mujun Lu, email: lumujun@163.com Wei Li, email: liwei1016_2000@sjtu.edu.cn Keywords: Bladder augmentation, adipose stem cell, silk fibroin, Bladder acellular matrix graft, angiogenesis Received: July 22, 2017 Accepted: August 28, 2017 Published: September 23, 2017 ABSTRACT The unfavourable clinical outcomes of host cell-seeded scaffolds for Bladder augmentation warrant improved bioactive biomaterials. This study aimed to examine the feasibility of adipose-derived stem cells (ASCs)-seeded bilayer Bladder acellular matrix graft (BAMG)-silk fibroin (SF) scaffold in enhancing Bladder Reconstruction. Sprague Dawley rats were randomly divided into three groups: the BAMG-SF-ASCs group, the acellular BAMG-SF group and the cystotomy group. The BAMG-SF-ASCs group was sampled at 2, 4 and 12 weeks, and compared with the other groups at 12 weeks. In the BAMG-SF-ASCs group, the normal Bladder contour was reformed similar to that in the cystotomy group, with abundant urothelium and smooth muscle regeneration, as well as a suitable scaffold degradation speed, and trivial fibrosis and inflammation. The ASCs seeded in BAMG-SF were maintained in the regenerated region during the 12-week experimental period and significantly enhanced the vessel density, nerve regeneration and Bladder function compared with acellular BAMG-SF. In addition, the BAMG-SF-ASCs group presented elevated levels of SDF-1α, VEGF and their receptors, with an obvious increase in ERK 1/2 phosphorylation. BAMG-SF is a promising biomaterial for ASCs seeding to facilitate Bladder augmentation and demonstrated an enhanced angiogenic potential possibly related to the SDF-1α/CXCR4 pathway via ERK 1/2 activation.
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comparison of morphological and functional restoration between asymmetric bilayer chitosan and Bladder acellular matrix graft for Bladder augmentation in a rat model
RSC Advances, 2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, An Qin, Yang Zhao, Ming Zhang, Zhe ZhouAbstract:Because of the limitations of current scaffolds and unfavorable results of clinical trials, proper scaffolds facilitating Bladder Reconstruction are highly desirable. The aim of this study was to evaluate a novel asymmetric bilayer chitosan scaffold compared with conventional Bladder acellular matrix graft (BAMG) in a rat model of Bladder augmentation. Twenty-four 8 week-old male Sprague-Dawley rats were randomly assigned to the chitosan scaffold, BAMG and cystotomy groups. The rats' Bladders were sampled for cystography and routine histological examination at 21 and 70 days. Immunofluorescence photometry, conscious cystometry, quantitative real-time polymerase chain reaction, and western blot analyses were performed using Bladders at 70 days. Compared with BAMG, the chitosan scaffold consisted of a membrane-like compact layer and a sponge-like porous layer with an excellent combination of mechanical strength and flexibility. The chitosan group showed better performances than the BAMG group in radiographic cystography, smooth muscle regeneration, blood vessel numbers and functional restoration. In contrast to reduced Bladder compliance induced by BAMG, Bladder augmented by chitosan displayed nearly 1.5-fold increased Bladder capacity with comparable compliance to that of the cystotomy group at 70 days. The chitosan group exhibited higher levels of VEGF and VEGFR2, associated with the activation of the hypoxia-related SDF-1α/CXCR4 pathway. These results suggested that the asymmetric bilayer chitosan scaffold is a promising scaffold for Bladder Reconstruction.
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Trilayer Three-Dimensional Hydrogel Composite Scaffold Containing Encapsulated Adipose-Derived Stem Cells Promotes Bladder Reconstruction via SDF-1α/CXCR4 Pathway
2017Co-Authors: Dongdong Xiao, Qiong Wang, Hao Yan, Yang Zhao, Ming Zhang, Zhe Zhou, Qian Sun, Kang SunAbstract:Bladder acellular matrix graft-alginate dialdehyde-gelatin hydrogel-silk mesh (BAMG-HS) encapsulated with adipose-derived stem cells (ASCs) was evaluated in a rat model of augmentation cystoplasty, including BAMG-HS-ASCs (n = 18, subgroup n = 6 for 2, 4, and 12 weeks), acellular BAMG-HS (n = 6 for 12 weeks) and cystotomy control (n = 6 for 12 weeks) groups. Equipped with good cytocompatibility and superior mechanical properties (elastic modulus: 5.33 ± 0.96 MPa, maximum load: 28.90 ± 0.69 N), BAMG-HS acted a trilayer “sandwich” scaffold with minimal interference in systemic homeostasis. ASCs in BAMG-HS promoted morphological and histological Bladder restoration by accelerating scaffold degradation (p < 0.05), ameliorating fibrosis (p < 0.05) and inflammation (p < 0.01). Additionally, ASCs facilitated the recovery of Bladder function by enhancing smooth muscle regeneration (p < 0.05), innervation (p < 0.01) and angiogenesis (p < 0.001). Except for a small number of endothelium-differentiated ASCs, the pro-angiogenic effects of ASCs were mainly related to ERK1/2 phosphorylation in the downstream of SDF-1α/CXCR4 pathway