The Experts below are selected from a list of 1068 Experts worldwide ranked by ideXlab platform
Mara E.m. Braga - One of the best experts on this subject based on the ideXlab platform.
-
effect of scco2 sorption capacity on the total amount of Borage Oil loaded by scco2 impregnation deposition into a polyurethane based wound dressing
Journal of Supercritical Fluids, 2016Co-Authors: C.v. Da Silva, Verônica J. Pereira, Paulo T.v. Rosa, G. M. N. Costa, A.m.a. Dias, H.c. De Sousa, Elaine C M Cabralalbuquerque, S Vieira A B De Melo, Mara E.m. BragaAbstract:Abstract In this work, experimental equilibrium solubility data of scCO 2 in polyurethane (PUR) foam (scCO 2 sorption capacity) was measured by a gravimetric method for different isotherms (308, 318 and 328 K), scCO 2 densities (650, 750 and 850 kg m −3 ) and scCO 2 exposure times (0.5, 2, 4, 8 and 16 h). Sorption data and CO 2 desorption coefficients were estimated from desorption curves that were modeled using both numerical and analytical solution of Fick’s second law, with deviations below 10% in both cases. The influence of scCO 2 sorption capacity on the amount of Borage Oil impregnated/deposited into the PUR foamed matrix was studied for selected pressure and temperature conditions and based on scCO 2 sorption data. Results showed that the highest solubility of scCO 2 in PUR (∼70%) was achieved at the lowest temperature and highest solvent density studied (308 K and 850 kg m −3 ) while the highest Borage Oil loaded yield was observed at the highest temperature and solvent density studied (328 K and 850 kg m −3 ), meaning that the impregnation/deposition process is controlled by the solubility of Borage Oil in scCO 2 . All the compounds present in Borage Oil (and which are detected by HPLC) were successfully loaded into the PUR matrix which confirms the feasibility of the impregnation/deposition process. Moreover, the amount of loaded Oil did not significantly affect the neat properties of the PUR matrix (mainly its hydrophobicity, thermal resistance and water vapor permeation capacity).
-
Effect of scCO2 sorption capacity on the total amount of Borage Oil loaded by scCO2 impregnation/deposition into a polyurethane-based wound dressing
The Journal of Supercritical Fluids, 2016Co-Authors: C.v. Da Silva, Verônica J. Pereira, Paulo T.v. Rosa, Elaine C. M. Cabral-albuquerque, S.a.b. Vieira De Melo, G. M. N. Costa, A.m.a. Dias, H.c. De Sousa, Mara E.m. BragaAbstract:Abstract In this work, experimental equilibrium solubility data of scCO 2 in polyurethane (PUR) foam (scCO 2 sorption capacity) was measured by a gravimetric method for different isotherms (308, 318 and 328 K), scCO 2 densities (650, 750 and 850 kg m −3 ) and scCO 2 exposure times (0.5, 2, 4, 8 and 16 h). Sorption data and CO 2 desorption coefficients were estimated from desorption curves that were modeled using both numerical and analytical solution of Fick’s second law, with deviations below 10% in both cases. The influence of scCO 2 sorption capacity on the amount of Borage Oil impregnated/deposited into the PUR foamed matrix was studied for selected pressure and temperature conditions and based on scCO 2 sorption data. Results showed that the highest solubility of scCO 2 in PUR (∼70%) was achieved at the lowest temperature and highest solvent density studied (308 K and 850 kg m −3 ) while the highest Borage Oil loaded yield was observed at the highest temperature and solvent density studied (328 K and 850 kg m −3 ), meaning that the impregnation/deposition process is controlled by the solubility of Borage Oil in scCO 2 . All the compounds present in Borage Oil (and which are detected by HPLC) were successfully loaded into the PUR matrix which confirms the feasibility of the impregnation/deposition process. Moreover, the amount of loaded Oil did not significantly affect the neat properties of the PUR matrix (mainly its hydrophobicity, thermal resistance and water vapor permeation capacity).
C.v. Da Silva - One of the best experts on this subject based on the ideXlab platform.
-
effect of scco2 sorption capacity on the total amount of Borage Oil loaded by scco2 impregnation deposition into a polyurethane based wound dressing
Journal of Supercritical Fluids, 2016Co-Authors: C.v. Da Silva, Verônica J. Pereira, Paulo T.v. Rosa, G. M. N. Costa, A.m.a. Dias, H.c. De Sousa, Elaine C M Cabralalbuquerque, S Vieira A B De Melo, Mara E.m. BragaAbstract:Abstract In this work, experimental equilibrium solubility data of scCO 2 in polyurethane (PUR) foam (scCO 2 sorption capacity) was measured by a gravimetric method for different isotherms (308, 318 and 328 K), scCO 2 densities (650, 750 and 850 kg m −3 ) and scCO 2 exposure times (0.5, 2, 4, 8 and 16 h). Sorption data and CO 2 desorption coefficients were estimated from desorption curves that were modeled using both numerical and analytical solution of Fick’s second law, with deviations below 10% in both cases. The influence of scCO 2 sorption capacity on the amount of Borage Oil impregnated/deposited into the PUR foamed matrix was studied for selected pressure and temperature conditions and based on scCO 2 sorption data. Results showed that the highest solubility of scCO 2 in PUR (∼70%) was achieved at the lowest temperature and highest solvent density studied (308 K and 850 kg m −3 ) while the highest Borage Oil loaded yield was observed at the highest temperature and solvent density studied (328 K and 850 kg m −3 ), meaning that the impregnation/deposition process is controlled by the solubility of Borage Oil in scCO 2 . All the compounds present in Borage Oil (and which are detected by HPLC) were successfully loaded into the PUR matrix which confirms the feasibility of the impregnation/deposition process. Moreover, the amount of loaded Oil did not significantly affect the neat properties of the PUR matrix (mainly its hydrophobicity, thermal resistance and water vapor permeation capacity).
-
Effect of scCO2 sorption capacity on the total amount of Borage Oil loaded by scCO2 impregnation/deposition into a polyurethane-based wound dressing
The Journal of Supercritical Fluids, 2016Co-Authors: C.v. Da Silva, Verônica J. Pereira, Paulo T.v. Rosa, Elaine C. M. Cabral-albuquerque, S.a.b. Vieira De Melo, G. M. N. Costa, A.m.a. Dias, H.c. De Sousa, Mara E.m. BragaAbstract:Abstract In this work, experimental equilibrium solubility data of scCO 2 in polyurethane (PUR) foam (scCO 2 sorption capacity) was measured by a gravimetric method for different isotherms (308, 318 and 328 K), scCO 2 densities (650, 750 and 850 kg m −3 ) and scCO 2 exposure times (0.5, 2, 4, 8 and 16 h). Sorption data and CO 2 desorption coefficients were estimated from desorption curves that were modeled using both numerical and analytical solution of Fick’s second law, with deviations below 10% in both cases. The influence of scCO 2 sorption capacity on the amount of Borage Oil impregnated/deposited into the PUR foamed matrix was studied for selected pressure and temperature conditions and based on scCO 2 sorption data. Results showed that the highest solubility of scCO 2 in PUR (∼70%) was achieved at the lowest temperature and highest solvent density studied (308 K and 850 kg m −3 ) while the highest Borage Oil loaded yield was observed at the highest temperature and solvent density studied (328 K and 850 kg m −3 ), meaning that the impregnation/deposition process is controlled by the solubility of Borage Oil in scCO 2 . All the compounds present in Borage Oil (and which are detected by HPLC) were successfully loaded into the PUR matrix which confirms the feasibility of the impregnation/deposition process. Moreover, the amount of loaded Oil did not significantly affect the neat properties of the PUR matrix (mainly its hydrophobicity, thermal resistance and water vapor permeation capacity).
Stephen J Demichele - One of the best experts on this subject based on the ideXlab platform.
-
comparison of growth serum biochemistries and n 6 fatty acid metabolism in rats fed diets supplemented with high gamma linolenic acid safflower Oil or Borage Oil for 90 days
Food and Chemical Toxicology, 2012Co-Authors: Jody L Caldwell, Gregory P Boivin, Stephen J DemicheleAbstract:Abstract Recently, steps have been taken to further developments toward increasing gamma-linolenic acid (GLA) concentration and lowering costs in plant seed Oils using transgenic technology. Through identification and expression of a fungal delta-6 desaturase gene in the high linoleic acid safflower plant, the seeds from this genetic transformation produce Oil with >40% GLA (high GLA safflower Oil (HGSO)). The aim of the study was to compare the effects of feeding HGSO to a generally recognized as safe source of GLA, Borage Oil, in a 90 day safety study in rats. Weanling male and female Sprague–Dawley rats were fed a semi-synthetic, fat free, pelleted diet (AIN93G) supplemented with a 10% (wt/wt) Oil blend containing HGSO or Borage Oil, with equivalent GLA levels. Results demonstrated that feeding diets containing HGSO or Borage Oil for 90 days had similar biologic effects with regard to growth characteristics, body composition, behavior, organ weight and histology, and parameters of hematology and serum biochemistries in both sexes. Metabolism of the primary n −6 fatty acids in plasma and organ phospholipids was similar, despite minor changes in females. We conclude that HGSO is biologically equivalent to Borage Oil and provides a safe alternative source of GLA in the diet.
-
Effect of Long-Term Dietary Supplementation of High-Gamma-Linolenic Canola Oil versus Borage Oil on Growth, Hematology, Serum Biochemistry, and N-6 Fatty Acid Metabolism in Rats
Journal of agricultural and food chemistry, 2004Co-Authors: Jimwen Liu, Stephen J Demichele, John D Palombo, Lu-te Chuang, C. Hastilow, Emil G. Bobik, Yungsheng HuangAbstract:Dietary supplementation of a high-gamma-linolenic acid canola Oil (HGCO) containing approximately 36% (w/w) of gamma-linolenic acid (GLA, 18:3n-6) from the seeds of a genetically transformed canola strain, was assessed for its long-term biological effects. Growing Sprague−Dawley rats (n = 30) were fed a purified AIN93G diet containing 5, 10, or 15% (w/w) of HGCO as the fat source. For comparison, a separate group of rats (n = 10) was given the diet containing 15% (w/w) of Borage Oil (BO), which contained 22% (w/w) of GLA. After 12 weeks of feeding, the growth, relative organ weights, hematology, and serum biochemistry were found to be similar among rats fed the 5, 10, and 15% HGCO diets. The GLA levels in plasma and liver phospholipids (PL) were also similar. However, the levels of GLA in peripheral tissues (muscle PL and adipose triacylglycerols) were significantly higher in rats fed the 10 and 15% HGCO diets than those fed the 5% HGCO diet. When the above biologic parameters were compared between the 15...
-
Effects of high-γ-linolenic acid canola Oil compared with Borage Oil on reproduction, growth, and brain and behavioral development in mice
Lipids, 2003Co-Authors: Patricia E. Wainwright, Stephen J Demichele, Jimwen Liu, Yungsheng Huang, Lu-te Chuang, H.-c. Xing, Jessica BiedermanAbstract:Previous research in rats and mice has suggested that γ-linolenic acid (GLA) derived from Borage Oil (BO: 23% GLA) may be an appropriate source for increasing levels of long-chain n−6 FA in the developing brain. Recently, transgenic technology has made available a highly enriched GLA seed Oil from the canola plant (HGCO: 36% GLA). The first objective of this study was to compare the effects of diets containing equal levels of GLA (23%) from either BO or HGCO on reproduction, pup development, and pup brain FA composition in mice. The second objective was to compare the effects of the HGCO diluted to 23% GLA (GLA-23) with those of undiluted HGCO containing 36% GLA (GLA-36). The diets were fed to the dams prior to conception and throughout pregnancy and lactation, as well as to the pups after weaning. The behavioral development of the pups was measured 12 d after birth, and anxiety in the adult male offspring was assessed using the plus maze. The findings show that despite equivalent levels of GLA, GLA-23 differed from BO in that it reduced pup body weight and was associated with a slight increase in neonatal pup attrition. However, there were no significant effects on pup behavioral development or on performance in the plus maze. An increase in dietary GLA resulted in an increase in brain 20∶4n−6 and 22∶4n−6, with a corresponding decrease in 22∶6n−3. Again, despite their similar levels of GLA, these effects tended to be larger in GLA-23 than in BO. In comparison with GLA-23, GLA-36 had larger effects on growth and brain FA composition but no differences with respect to effects on reproduction and behavioral development. These findings suggest that the HGCO can be used as an alternative source of GLA.
-
comparison of growth and fatty acid metabolism in rats fed diets containing equal levels of γ linolenic acid from high γ linolenic acid canola Oil or Borage Oil
Lipids, 2000Co-Authors: John D Palombo, Stephen J Demichele, Jimwen Liu, Bruce R Bistrian, Yungsheng HuangAbstract:We have utilized transgenic technology to develop a new source of γ-linolenic acid (GLA) using the canola plant as a host. The aim of the present study was to compare the growth and fatty acid metabolism in rats fed equal amounts of GLA obtained from the transgenic canola plant relative to GLA from the Borage plant. Young male Sprague-Dawley rats (n=10/group) were randomized and fed a purified AIN93G diet (10% lipid by weight) containing either a mixture of high GLA canola Oil (HGCO) and corn Oil or a control diet containing Borage Oil (BO) for 6 wk. GLA accounted for 23% of the triglyceride fatty acids in both diets. Growth and diet consumption were monitored every 2–3 d throughout the study. At study termination, the fatty acid composition of the liver and plasma phospholipids was analyzed by gas chromatography. The growth and diet consumption of the HGCO group were similar to the BO group. There were no adverse effects of either diet on the general health or appearance of the rats, or on the morphology of the major organs. There was no significant difference between the diet groups for total percentage of n−6 polyunsaturated fatty acids present in either the total or individual phospholipid fractions of liver or plasma. The relative percentage of GLA and its main metabolite, arachidonic acid, in each phospholipid fraction of liver or plasma were also similar between groups. The percentage of 18∶2n−6 in liver phosphatidylethanolamine and phosphatidylinositol/serine was higher (P<0.05) and 22∶5n−6 was lower in the HGCO group than the BO group. This finding could be attributed to the higher 18∶3n−3 content in the HGCO diet than the BO diet. Results from this long-term feeding study of rats show for the first time that a diet containing transgenically modified canola Oil was well-tolerated, and had similar biological effects, i.e., growth characteristics and hepatic metabolism of n−6 fatty acids, as a diet containing Borage Oil.
-
Comparison of growth and fatty acid metabolism in rats fed diets containing equal levels of γ-linolenic acid from high γ-linolenic acid canola Oil or Borage Oil.
Lipids, 2000Co-Authors: John D Palombo, Stephen J Demichele, Jimwen Liu, Bruce R Bistrian, Yungsheng HuangAbstract:We have utilized transgenic technology to develop a new source of γ-linolenic acid (GLA) using the canola plant as a host. The aim of the present study was to compare the growth and fatty acid metabolism in rats fed equal amounts of GLA obtained from the transgenic canola plant relative to GLA from the Borage plant. Young male Sprague-Dawley rats (n=10/group) were randomized and fed a purified AIN93G diet (10% lipid by weight) containing either a mixture of high GLA canola Oil (HGCO) and corn Oil or a control diet containing Borage Oil (BO) for 6 wk. GLA accounted for 23% of the triglyceride fatty acids in both diets. Growth and diet consumption were monitored every 2–3 d throughout the study. At study termination, the fatty acid composition of the liver and plasma phospholipids was analyzed by gas chromatography. The growth and diet consumption of the HGCO group were similar to the BO group. There were no adverse effects of either diet on the general health or appearance of the rats, or on the morphology of the major organs. There was no significant difference between the diet groups for total percentage of n−6 polyunsaturated fatty acids present in either the total or individual phospholipid fractions of liver or plasma. The relative percentage of GLA and its main metabolite, arachidonic acid, in each phospholipid fraction of liver or plasma were also similar between groups. The percentage of 18∶2n−6 in liver phosphatidylethanolamine and phosphatidylinositol/serine was higher (P
Michael D Karlstad - One of the best experts on this subject based on the ideXlab platform.
-
dietary fish Oil and fish and Borage Oil suppress intrapulmonary proinflammatory eicosanoid biosynthesis and attenuate pulmonary neutrophil accumulation in endotoxic rats
Critical Care Medicine, 1997Co-Authors: Peter Mancuso, Jay Whelan, Stephen J Demichele, Carolyn C Snider, Julie A Guszcza, Michael D KarlstadAbstract:Objective: Proinflammatory eicosanoids and cytokines are important mediators of local inflammation in acute lung injury. We determined if enteral nutrition with anti-inflammatory fatty acids, eicosapentaenoic acid, and γ-linolenic acid would reduce the intrapulmonary synthesis of proinflammatory eicosanoids and cytokines and pulmonary neutrophil accumulation in a rat model of acute lung injury. Design: Prospective, randomized, controlled, double-blind study. Setting: Research laboratory at a university medical center. Subjects: Male Long-Evans rats (250 g). Interventions: Rats were randomly assigned to three dietary treatment groups and fed nutritionally complete diets (300 kcal/kg/ day) containing 55.2% of the total calories from fat with either 97% corn Oil, 20% fish Oil, or 20% fish and 20% Borage Oil for 21 days. On day 22, bronchoalveolar lavage was performed 2 hrs after an intravenous injection of Salmonella enteritidis endotoxin (10 mg/ kg) or saline. Bronchoalveolar lavage fluid was analyzed for leukotriene B 4 , leukotriene C 4 /D 4 , thromboxane B 2 , prostaglandin E 2 , 6 keto-prostaglandin F 1α , tumor necrosis factor (TNF)-α, and macrophage inflammatory protein-2 (MIP-2). Lung myeloperoxidase activity (a marker for neutrophil accumulation) and phospholipid fatty acid composition were also determined. Measurements and Main Results: Lung phospholipid concentrations of arachidonic acid were lower and the concentrations of eicosapentaenoic acid and docosahexaenoic acid were higher with fish Oil and fish and Borage Oil as compared with corn Oil. Dihomo-γ-linolenic acid, the desaturated and elongated intermediate of γ-linolenic acid, increased with fish and Borage Oil as compared with fish Oil and corn Oil. The levels of leukotriene B 4 , leukotriene C 4 /D 4 , 6-keto-prostaglandin F 1α and thromboxane B 2 with corn Oil were significantly increased with endotoxin as compared with saline. In contrast to the corn Oil group, endotoxin did not significantly increase bronchoalveolar lavage levels of leukotriene B 4 , leukotriene C 4 /D 4 , and thromboxane B 2 above those of saline-treated rats with fish Oil and fish and Borage Oil. Lung myeloperoxidase activity was significantly increased in endotoxin-treated rats compared with those rats given saline in all dietary treatment groups. However, lung myeloperoxidase activity was significantly lower with either fish Oil or fish and Borage Oil as compared with corn Oil after endotoxin. Although endotoxin increased the levels of TNF-α and MIP-2 with all dietary treatment groups as compared with saline-treated rats, there were no significant differences in the levels of either cytokine between the dietary treatment groups. Conclusions: These results indicate that dietary fish Oil and fish and Borage Oil as compared with corn Oil may ameliorate endotoxin-induced acute lung injury by suppressing the levels of proinflammatory eicosanoids (but not TNF-α or MIP-2) in bronchoalveolar lavage fluid and reducing pulmonary neutrophil accumulation.
-
Dietary fish Oil and fish and Borage Oil suppress intrapulmonary proinflammatory eicosanoid biosynthesis and attenuate pulmonary neutrophil accumulation in endotoxic rats
Critical care medicine, 1997Co-Authors: Peter Mancuso, Jay Whelan, Stephen J Demichele, Carolyn C Snider, Julie A Guszcza, Michael D KarlstadAbstract:Objective: Proinflammatory eicosanoids and cytokines are important mediators of local inflammation in acute lung injury. We determined if enteral nutrition with anti-inflammatory fatty acids, eicosapentaenoic acid, and γ-linolenic acid would reduce the intrapulmonary synthesis of proinflammatory eicosanoids and cytokines and pulmonary neutrophil accumulation in a rat model of acute lung injury. Design: Prospective, randomized, controlled, double-blind study. Setting: Research laboratory at a university medical center. Subjects: Male Long-Evans rats (250 g). Interventions: Rats were randomly assigned to three dietary treatment groups and fed nutritionally complete diets (300 kcal/kg/ day) containing 55.2% of the total calories from fat with either 97% corn Oil, 20% fish Oil, or 20% fish and 20% Borage Oil for 21 days. On day 22, bronchoalveolar lavage was performed 2 hrs after an intravenous injection of Salmonella enteritidis endotoxin (10 mg/ kg) or saline. Bronchoalveolar lavage fluid was analyzed for leukotriene B 4 , leukotriene C 4 /D 4 , thromboxane B 2 , prostaglandin E 2 , 6 keto-prostaglandin F 1α , tumor necrosis factor (TNF)-α, and macrophage inflammatory protein-2 (MIP-2). Lung myeloperoxidase activity (a marker for neutrophil accumulation) and phospholipid fatty acid composition were also determined. Measurements and Main Results: Lung phospholipid concentrations of arachidonic acid were lower and the concentrations of eicosapentaenoic acid and docosahexaenoic acid were higher with fish Oil and fish and Borage Oil as compared with corn Oil. Dihomo-γ-linolenic acid, the desaturated and elongated intermediate of γ-linolenic acid, increased with fish and Borage Oil as compared with fish Oil and corn Oil. The levels of leukotriene B 4 , leukotriene C 4 /D 4 , 6-keto-prostaglandin F 1α and thromboxane B 2 with corn Oil were significantly increased with endotoxin as compared with saline. In contrast to the corn Oil group, endotoxin did not significantly increase bronchoalveolar lavage levels of leukotriene B 4 , leukotriene C 4 /D 4 , and thromboxane B 2 above those of saline-treated rats with fish Oil and fish and Borage Oil. Lung myeloperoxidase activity was significantly increased in endotoxin-treated rats compared with those rats given saline in all dietary treatment groups. However, lung myeloperoxidase activity was significantly lower with either fish Oil or fish and Borage Oil as compared with corn Oil after endotoxin. Although endotoxin increased the levels of TNF-α and MIP-2 with all dietary treatment groups as compared with saline-treated rats, there were no significant differences in the levels of either cytokine between the dietary treatment groups. Conclusions: These results indicate that dietary fish Oil and fish and Borage Oil as compared with corn Oil may ameliorate endotoxin-induced acute lung injury by suppressing the levels of proinflammatory eicosanoids (but not TNF-α or MIP-2) in bronchoalveolar lavage fluid and reducing pulmonary neutrophil accumulation.
-
Effects of eicosapentaenoic and gamma-linolenic acid on lung permeability and alveolar macrophage eicosanoid synthesis in endotoxic rats.
Critical care medicine, 1997Co-Authors: Peter Mancuso, Jay Whelan, Stephen J Demichele, Carolyn C Snider, Julie A Guszcza, Kate J. Claycombe, Gary T. Smith, Gregory Timothy James, Michael D KarlstadAbstract:Objectives: Proinflammatory eicosanoids (cyclooxygenase and lipoxygenase metabolites of arachidonic acid) released by alveolar macrophages play an important role in endotoxin-induced acute lung injury. We investigated the effect of prefeeding rats for 21 days with enteral diets that provided the anti-inflammatory fatty acids, eicosapentaenoic acid and γ-linolenic acid (derived from fish Oil and Borage Oil, respectively), as compared with an n-6 fatty acid-enriched diet (corn Oil) on the following: a) lung microvascular protein permeability, arterial blood pressure, and platelet and white blood cells in a model of endotoxin-induced acute lung injury; b) alveolar macrophage prostaglandin and leukotriene synthesis; and c) liver and alveolar macrophage phospholipid fatty acid composition. Design: Prospective, randomized, controlled, double-blind study. Setting: Research laboratory at a university medical center. Subjects: Male Long-Evans rats, weighing 250 g. Interventions: Rats were randomized into four dietary treatment groups and fed nutritionally complete diets (300 kcal/kg/day), containing 55.2% of the total calories from fat with either 97% corn Oil, 20% fish Oil, 20% fish and 5% Borage Oil, or 20% fish and 20% Borage Oil for 21 days. On day 22, lung microvascular protein permeability, mean arterial pressure, and platelet and white blood cell counts were determined for 2 hrs after an intravenous injection of Salmonella enteritidis endotoxin (10 mg/kg). In a second group of prefed rats, the phospholipid fatty acid composition was determined in liver and alveolar macrophages. Alveolar macrophages were harvested by bronchoalveolar lavage and stimulated in vitro with a calcium ionophore (A23187), and the concentrations of leukotrienes B 4 and B 5, thromboxane A 2 , prostaglandin E 2 , and 6keto-prostaglandin F 1α were measured in a third group of prefed rats. Measurements and Main Results: Lung permeability was greatest with corn Oil and was significantly attenuated with 20% fish Oil and 20% fish and 5% Borage Oil, and this effect approached significance with 20% fish and 20% Borage Oil (p =.06). The early and late hypotensive effects of endotoxin were attenuated with 20% fish Oil, 20% fish and 5% Borage Oil, and 20% fish and 20% Borage Oil, as compared with corn Oil. Concentrations of leukotriene B 4 , prostaglandin E 2 , and thromboxane B 2 released from A23187-stimulated alveolar macrophages were significantly lower with 20% fish Oil and 20% fish and 20% Borage Oil, as compared with corn Oil. The increase in lung microvascular protein permeability with 20% fish and 20% Borage Oil was not significantly different than the lung microvascular protein permeability that was found in animals receiving 20% fish Oil (p =.20) and 20% fish and 5% Borage Oil (p = .31). Alveolar macrophage and liver phospholipid concentrations of arachidonic acid were lower, and the concentrations of eicosapentaenoic acid and docosahexaenoic acid were higher, with 20% fish Oil, 20% fish and 5% Borage Oil, and 20% fish and 20% Borage Oil, as compared with corn Oil. Dihomo-γ-linolenic acid, the desaturated and elongated intermediate of γ-linolenic acid, was increased with 20% fish and 20% Borage Oil, as compared with 20% fish Oil and 20% fish and 5% Borage Oil. Conclusions: The severity of pulmonary microvascular protein permeability and the degree of hypotension were reduced with fish or fish and Borage Oil diets, as compared with corn Oil, in endotoxic rats. The reduced synthesis of the proinflammatory arachidonic acid-derived mediators, leukotriene B 4 , thromboxane B 2 , and prostaglandin E 2 from stimulated alveolar macrophages was indicative of a decrease in arachidonic acid and an increase in eicosapentaenoic acid and docosahexaenoic acid in cell membrane phospholipids. The modulation of proinflammatory eicosanoid synthesis with anti-inflammatory fatty acids, eicosapentaenoic acid, and docosapentaenoic acid and γ-linolenic acid, may have reduced the seve
H.c. De Sousa - One of the best experts on this subject based on the ideXlab platform.
-
effect of scco2 sorption capacity on the total amount of Borage Oil loaded by scco2 impregnation deposition into a polyurethane based wound dressing
Journal of Supercritical Fluids, 2016Co-Authors: C.v. Da Silva, Verônica J. Pereira, Paulo T.v. Rosa, G. M. N. Costa, A.m.a. Dias, H.c. De Sousa, Elaine C M Cabralalbuquerque, S Vieira A B De Melo, Mara E.m. BragaAbstract:Abstract In this work, experimental equilibrium solubility data of scCO 2 in polyurethane (PUR) foam (scCO 2 sorption capacity) was measured by a gravimetric method for different isotherms (308, 318 and 328 K), scCO 2 densities (650, 750 and 850 kg m −3 ) and scCO 2 exposure times (0.5, 2, 4, 8 and 16 h). Sorption data and CO 2 desorption coefficients were estimated from desorption curves that were modeled using both numerical and analytical solution of Fick’s second law, with deviations below 10% in both cases. The influence of scCO 2 sorption capacity on the amount of Borage Oil impregnated/deposited into the PUR foamed matrix was studied for selected pressure and temperature conditions and based on scCO 2 sorption data. Results showed that the highest solubility of scCO 2 in PUR (∼70%) was achieved at the lowest temperature and highest solvent density studied (308 K and 850 kg m −3 ) while the highest Borage Oil loaded yield was observed at the highest temperature and solvent density studied (328 K and 850 kg m −3 ), meaning that the impregnation/deposition process is controlled by the solubility of Borage Oil in scCO 2 . All the compounds present in Borage Oil (and which are detected by HPLC) were successfully loaded into the PUR matrix which confirms the feasibility of the impregnation/deposition process. Moreover, the amount of loaded Oil did not significantly affect the neat properties of the PUR matrix (mainly its hydrophobicity, thermal resistance and water vapor permeation capacity).
-
Effect of scCO2 sorption capacity on the total amount of Borage Oil loaded by scCO2 impregnation/deposition into a polyurethane-based wound dressing
The Journal of Supercritical Fluids, 2016Co-Authors: C.v. Da Silva, Verônica J. Pereira, Paulo T.v. Rosa, Elaine C. M. Cabral-albuquerque, S.a.b. Vieira De Melo, G. M. N. Costa, A.m.a. Dias, H.c. De Sousa, Mara E.m. BragaAbstract:Abstract In this work, experimental equilibrium solubility data of scCO 2 in polyurethane (PUR) foam (scCO 2 sorption capacity) was measured by a gravimetric method for different isotherms (308, 318 and 328 K), scCO 2 densities (650, 750 and 850 kg m −3 ) and scCO 2 exposure times (0.5, 2, 4, 8 and 16 h). Sorption data and CO 2 desorption coefficients were estimated from desorption curves that were modeled using both numerical and analytical solution of Fick’s second law, with deviations below 10% in both cases. The influence of scCO 2 sorption capacity on the amount of Borage Oil impregnated/deposited into the PUR foamed matrix was studied for selected pressure and temperature conditions and based on scCO 2 sorption data. Results showed that the highest solubility of scCO 2 in PUR (∼70%) was achieved at the lowest temperature and highest solvent density studied (308 K and 850 kg m −3 ) while the highest Borage Oil loaded yield was observed at the highest temperature and solvent density studied (328 K and 850 kg m −3 ), meaning that the impregnation/deposition process is controlled by the solubility of Borage Oil in scCO 2 . All the compounds present in Borage Oil (and which are detected by HPLC) were successfully loaded into the PUR matrix which confirms the feasibility of the impregnation/deposition process. Moreover, the amount of loaded Oil did not significantly affect the neat properties of the PUR matrix (mainly its hydrophobicity, thermal resistance and water vapor permeation capacity).