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Sven Bergstrom – 1st expert on this subject based on the ideXlab platform
global ecology and epidemiology of Borrelia garinii spirochetesInfection ecology & epidemiology, 2011Co-Authors: Par Comstedt, Tobias Jakobsson, Sven BergstromAbstract:
Lyme borreliosis (LB) is a tick-transmitted infectious disease caused by Borrelia burgdorferi sensu lato (s. l.). In Europe, three different Borrelia species are the main causative agents of LB: B. burgdorferi sensu stricto (s.s.), Borrelia afzelii, and Borrelia garinii. The latter depends heavily on birds as its main reservoir hosts. In fact, birds can act both as biological carriers of Borrelia and transporters of infected ticks. The seasonal migration of many bird species not only aid in the spread of B. garinii to new foci but also influence the high level of diversity found within this species. B. garinii have been isolated not only from terrestrial birds in Europe, but also from seabirds worldwide, and homology between isolates in these two different infection cycles suggests an overlap and exchange of strains. In addition, it has been shown that birds can maintain and spread B. garinii genotypes associated with LB in humans. This review article discusses the importance of birds in the ecology and epidemiology of B. garinii spirochetes.
complex population structure of lyme borreliosis group spirochete Borrelia garinii in subarctic eurasiaPLOS ONE, 2009Co-Authors: Par Comstedt, Bjorn R Olsen, Loreta Asokliene, Ingvar Eliasson, Anders Wallensten, Jonas Bunikis, Sven BergstromAbstract:
Borrelia garinii, a causative agent of Lyme borreliosis in Europe and Asia, is naturally maintained in marine and terrestrial enzootic cycles, which primarily involve birds, including seabirds and …
Borrelia garinii isolated from seabirds can infect rodents but is sensitive to normal human serum, 2008Co-Authors: Par Comstedt, Christer Larsson, Taru Meri, Seppo Meri, Sven BergstromAbstract:
Borrelia garinii isolated from seabirds can infect rodents but is sensitive to normal human serum
Nikolaevich Vladislav Fomenko – 2nd expert on this subject based on the ideXlab platform
Antibodies from naive and multiple sclerosis phage display libraries bind Borrelia garinii antigensFEBS Journal, 2013Co-Authors: Vera V Morozova, Nikolaevich Vladislav Fomenko, Andrey L. Matveev, Oleg V. Stronin, Nina V. TikunovaAbstract:
Multiple sclerosis (MS) and progressive encephalomyelitis, a sequela of Lyme borreliosis, have similar clinical symptoms and are accompanied with appearance of multifocal central nervous system lesions. Multiple sclerosis is associated with autoantibodies, specific to myelin proteins and anti-myelin antibodies are present in some cases of neuroborreliosis. To investigate the reasons of the immune disorder the reperoires of antibodies, specific to Borrelia garinii from MS patients and healthy controls were compared. Phage display naive library of antibodies produced using mRNA from peripheral blood lymphocytes of healthy volunteers and MS-library of antibodies of multiple sclerosis patients, were panned against Borrelia garinii antigens. B. gariniispecific antibodies were selected and gene segments, encoding varible fragments of antibodies were sequenced. Gene segments from naïve library were highly homologous to germline genes, at the same time more than a third of gene segments encoding B. garinii-specific antibodies from MS-library possessed low homology with germline genes. Gene segment VH4-39, specific for intrathecal synthesis, and VH6, normally expressing in embryo, were identified among gene segments, encoding B. garinii– specific antibodies from MS-library. Populations of antibodies enriched against B. garinii antigens, effectively bound mielin basic protein in ELISA. Probably, B. garinii proteins may cause autoimmune reaction against nervous system, using molecular mimicry mechanism.
whole genome sequencing of Borrelia garinii bgvir isolated from taiga ticks ixodes persulcatusJournal of Bacteriology, 2012Co-Authors: Evgeniy V Brenner, Oleg V. Stronin, Alexander Kurilshikov, Nikolaevich Vladislav FomenkoAbstract:
ABSTRACT Most Lyme borreliosis cases in Russia result from Borrelia garinii NT29 group infection. Borrelias of this group circulate exclusively in Ixodes persulcatus ticks, which are seldom found beyond Russia and the far east. Here we report the whole-genome sequence of Borrelia garinii BgVir isolated from an I. persulcatus female.
Heterogeneity of the ospA gene structure from isolates of Borrelia garinii and Borrelia afzelii from Western Siberia and MongoliaMolecular Genetics Microbiology and Virology, 2009Co-Authors: Nikolaevich Vladislav Fomenko, Oleg V. Stronin, M A Khasnatinov, G A Danchinova, J Bataa, N. A. Gol’tsovaAbstract:
In this work, identification and analyses of 48 full-length sequences of the ospA gene isolates of Borrelia garinii and Borrelia afzelii from Western Siberia and Mongolia has been made. It was shown that B. garinii isolates was of its high genetic heterogeneity of the ospA gene. Four genetic groups of the ospA gene from the Ixodes persulcatus tick collected in of Western Siberia and Mongolia were defined. The basic differences in the genetic variants of the ospA gene considered are seen in regions which code for antibody determinants of thhe OspA protein.
Bettina Wilske – 3rd expert on this subject based on the ideXlab platform
adhesion of Borrelia garinii to neuronal cells is mediated by the interaction of ospa with proteoglycansJournal of Neuroimmunology, 2006Co-Authors: Tobias A Rupprecht, Uwe Koedel, Christiane Heimerl, Volker Fingerle, Robert Paul, Bettina Wilske, Hanswalter PfisterAbstract:
Abstract To study pathogenic mechanisms of Lyme meningoradiculitis, dorsal root ganglia (DRG) cells and two neuronal cell lines (B50, SH-SY5Y) were incubated with Borrelia garinii , the Borrelia species most frequently isolated from CSF of Lyme neuroborreliosis patients in Europe. We demonstrated that (I) OspA-positive B. garinii adhere to neuronal cells, (II) Borrelia adhesion can be blocked by a monoclonal antibody against OspA, (III) preincubation with proteoglycans interferes with the adhesion process and (IV) rOspA directly binds to the proteoglycans. This indicates that both OspA and the cell bound proteoglycans are involved in the attachment of B. garinii to neuronal cells.
Prevalence of Four Species of Borrelia burgdorferi Sensu Lato and Coinfection with Anaplasma phagocytophila in Ixodes ricinus Ticks in Central GermanyEuropean Journal of Clinical Microbiology & Infectious Diseases, 2003Co-Authors: Anke Hildebrandt, Bettina Wilske, Karl-hermann Schmidt, W. Dorn, Eberhard Straube, Volker FingerleAbstract:
A total of 305 Ixodes ricinus ticks collected from three areas of Thuringia in central Germany were investigated for infection with Borrelia burgdorferi sensu lato species and Anaplasma phagocytophila. Overall, 11.1% were infected with Borrelia burgdorferi and 2.3% with Anaplasma phagocytophila. Adult ticks showed a significantly higher rate of infection with both Borreliae and Anaplasma phagocytophila. Borrelia garinii (55.9%) was detected most frequently, followed by Borrelia burgdorferi sensu stricto (32.4%), Borrelia afzelii (17.6%), and Borrelia valaisiana (5.9%). Four ticks had dual infection with Borrelia garinii and Borrelia burgdorferi sensu stricto. Two of the Borrelia-positive ticks were coinfected with Anaplasma phagocytophila.
apodemus species mice are reservoir hosts of Borrelia garinii ospa serotype 4 in switzerlandJournal of Clinical Microbiology, 2002Co-Authors: D Huegli, Bettina Wilske, Chang Min Hu, Pierrefrancois Humair, Lise GernAbstract:
Among Borrelia burgdorferi sensu lato isolates, seven outer surface protein A (OspA) serotypes have been described: serotypes 1 and 2 correspond to B. burgdorferi sensu stricto and Borrelia afzelii, respectively, and serotypes 3 to 7 correspond to Borrelia garinii. In Europe, serotype 4 has never been isolated from Ixodes ricinus ticks until recently, although this serotype has been frequently isolated from cerebrospinal fluid from patients. In Europe, B. afzelii and B. burgdorferi sensu stricto were found associated with rodents and B. garinii was found associated with birds. In this study, the reservoir role of Apodemus mice for B. garinii OspA serotype 4 was demonstrated by xenodiagnosis. Apodemus mice are the first identified reservoir hosts for B. garinii OspA serotype 4.