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Sven Bergstrom - One of the best experts on this subject based on the ideXlab platform.
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global ecology and epidemiology of Borrelia garinii spirochetes
Infection ecology & epidemiology, 2011Co-Authors: Par Comstedt, Tobias Jakobsson, Sven BergstromAbstract:Lyme borreliosis (LB) is a tick-transmitted infectious disease caused by Borrelia burgdorferi sensu lato (s. l.). In Europe, three different Borrelia species are the main causative agents of LB: B. burgdorferi sensu stricto (s.s.), Borrelia afzelii, and Borrelia garinii. The latter depends heavily on birds as its main reservoir hosts. In fact, birds can act both as biological carriers of Borrelia and transporters of infected ticks. The seasonal migration of many bird species not only aid in the spread of B. garinii to new foci but also influence the high level of diversity found within this species. B. garinii have been isolated not only from terrestrial birds in Europe, but also from seabirds worldwide, and homology between isolates in these two different infection cycles suggests an overlap and exchange of strains. In addition, it has been shown that birds can maintain and spread B. garinii genotypes associated with LB in humans. This review article discusses the importance of birds in the ecology and epidemiology of B. garinii spirochetes.
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complex population structure of lyme borreliosis group spirochete Borrelia garinii in subarctic eurasia
PLOS ONE, 2009Co-Authors: Par Comstedt, Bjorn R Olsen, Loreta Asokliene, Ingvar Eliasson, Anders Wallensten, Jonas Bunikis, Sven BergstromAbstract:Borrelia garinii, a causative agent of Lyme borreliosis in Europe and Asia, is naturally maintained in marine and terrestrial enzootic cycles, which primarily involve birds, including seabirds and ...
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Borrelia garinii isolated from seabirds can infect rodents but is sensitive to normal human serum
2008Co-Authors: Par Comstedt, Christer Larsson, Taru Meri, Seppo Meri, Sven BergstromAbstract:Borrelia garinii isolated from seabirds can infect rodents but is sensitive to normal human serum
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Phylogeographic Relationships of Zxodes Uriae (Acari: Ixodidae) and Their Significance to Transequatorial Dispersal of Borrelia garinii
Hereditas, 2004Co-Authors: Åsa Gylfe, Mihe Yabuki, Marcus K. Drotz, Masahito Fukunaga, Sven Bergstrom, Bjorn R OlsenAbstract:Phylogenetic relationship of Ixodes uriae (Acari: Ixodidae) and their significance to transequatorial dispersal of Borrelia garinii
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augmented intrathecal secretion of interferon γ in response to Borrelia garinii in neuroborreliosis
Journal of Neuroimmunology, 1998Co-Authors: Christina Ekerfelt, Jan Ernerudh, Pia Forsberg, Sven BergstromAbstract:Abstract The Lyme disease agent Borrelia garinii has been suggested to be neurotrop, preferentially affecting the nervous system. We compared the secretion of interferon-γ in response to outer surface proteins from Borrelia garinii and Borrelia afzelii in 10 patients with neuroborreliosis. In cerebrospinal fluid, stimulation with Borrelia garinii revealed higher numbers of interferon-γ-secreting cells in all patients, whereas in blood, only five displayed higher numbers. This further strengthens the hypothesis of Borrelia garinii being associated with the development of neuroborreliosis.
Nikolaevich Vladislav Fomenko - One of the best experts on this subject based on the ideXlab platform.
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Antibodies from naive and multiple sclerosis phage display libraries bind Borrelia garinii antigens
FEBS Journal, 2013Co-Authors: Vera V Morozova, Nikolaevich Vladislav Fomenko, Andrey L. Matveev, Oleg V. Stronin, Nina V. TikunovaAbstract:Multiple sclerosis (MS) and progressive encephalomyelitis, a sequela of Lyme borreliosis, have similar clinical symptoms and are accompanied with appearance of multifocal central nervous system lesions. Multiple sclerosis is associated with autoantibodies, specific to myelin proteins and anti-myelin antibodies are present in some cases of neuroborreliosis. To investigate the reasons of the immune disorder the reperoires of antibodies, specific to Borrelia garinii from MS patients and healthy controls were compared. Phage display naive library of antibodies produced using mRNA from peripheral blood lymphocytes of healthy volunteers and MS-library of antibodies of multiple sclerosis patients, were panned against Borrelia garinii antigens. B. gariniispecific antibodies were selected and gene segments, encoding varible fragments of antibodies were sequenced. Gene segments from naïve library were highly homologous to germline genes, at the same time more than a third of gene segments encoding B. garinii-specific antibodies from MS-library possessed low homology with germline genes. Gene segment VH4-39, specific for intrathecal synthesis, and VH6, normally expressing in embryo, were identified among gene segments, encoding B. garinii- specific antibodies from MS-library. Populations of antibodies enriched against B. garinii antigens, effectively bound mielin basic protein in ELISA. Probably, B. garinii proteins may cause autoimmune reaction against nervous system, using molecular mimicry mechanism.
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whole genome sequencing of Borrelia garinii bgvir isolated from taiga ticks ixodes persulcatus
Journal of Bacteriology, 2012Co-Authors: Evgeniy V Brenner, Oleg V. Stronin, Alexander Kurilshikov, Nikolaevich Vladislav FomenkoAbstract:ABSTRACT Most Lyme borreliosis cases in Russia result from Borrelia garinii NT29 group infection. Borrelias of this group circulate exclusively in Ixodes persulcatus ticks, which are seldom found beyond Russia and the far east. Here we report the whole-genome sequence of Borrelia garinii BgVir isolated from an I. persulcatus female.
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Heterogeneity of the ospA gene structure from isolates of Borrelia garinii and Borrelia afzelii from Western Siberia and Mongolia
Molecular Genetics Microbiology and Virology, 2009Co-Authors: Nikolaevich Vladislav Fomenko, Oleg V. Stronin, M A Khasnatinov, G A Danchinova, J Bataa, N. A. Gol’tsovaAbstract:In this work, identification and analyses of 48 full-length sequences of the ospA gene isolates of Borrelia garinii and Borrelia afzelii from Western Siberia and Mongolia has been made. It was shown that B. garinii isolates was of its high genetic heterogeneity of the ospA gene. Four genetic groups of the ospA gene from the Ixodes persulcatus tick collected in of Western Siberia and Mongolia were defined. The basic differences in the genetic variants of the ospA gene considered are seen in regions which code for antibody determinants of thhe OspA protein.
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the heterogeneity of the ospa gene of Borrelia garinii and Borrelia afzelii in western siberia and mongolia
Molekuliarnaia genetika mikrobiologiia i virusologiia, 2009Co-Authors: Nikolaevich Vladislav Fomenko, Oleg V. Stronin, M A Khasnatinov, G A Danchinova, J Bataa, N A GoltsovaAbstract:: 48 full-length Borrelia garinii and Borrelia afzelii from West Siberia and Mongolia ospA gene nucleotide sequences was determined. Four groups of Borrelia garinii were revealed using the analysis of nucleotide sequences. The most variable ospA gene region was demonstrated to be included in region where the antigenic determinants of protein were encoded. High homology level was shown for nucleotide sequences corresponding to isolates of Borrelia afzelii.
Bettina Wilske - One of the best experts on this subject based on the ideXlab platform.
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adhesion of Borrelia garinii to neuronal cells is mediated by the interaction of ospa with proteoglycans
Journal of Neuroimmunology, 2006Co-Authors: Tobias A Rupprecht, Uwe Koedel, Christiane Heimerl, Volker Fingerle, Robert Paul, Bettina Wilske, Hanswalter PfisterAbstract:Abstract To study pathogenic mechanisms of Lyme meningoradiculitis, dorsal root ganglia (DRG) cells and two neuronal cell lines (B50, SH-SY5Y) were incubated with Borrelia garinii , the Borrelia species most frequently isolated from CSF of Lyme neuroborreliosis patients in Europe. We demonstrated that (I) OspA-positive B. garinii adhere to neuronal cells, (II) Borrelia adhesion can be blocked by a monoclonal antibody against OspA, (III) preincubation with proteoglycans interferes with the adhesion process and (IV) rOspA directly binds to the proteoglycans. This indicates that both OspA and the cell bound proteoglycans are involved in the attachment of B. garinii to neuronal cells.
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Prevalence of Four Species of Borrelia burgdorferi Sensu Lato and Coinfection with Anaplasma phagocytophila in Ixodes ricinus Ticks in Central Germany
European Journal of Clinical Microbiology & Infectious Diseases, 2003Co-Authors: Anke Hildebrandt, Bettina Wilske, Karl-hermann Schmidt, W. Dorn, Eberhard Straube, Volker FingerleAbstract:A total of 305 Ixodes ricinus ticks collected from three areas of Thuringia in central Germany were investigated for infection with Borrelia burgdorferi sensu lato species and Anaplasma phagocytophila. Overall, 11.1% were infected with Borrelia burgdorferi and 2.3% with Anaplasma phagocytophila. Adult ticks showed a significantly higher rate of infection with both Borreliae and Anaplasma phagocytophila. Borrelia garinii (55.9%) was detected most frequently, followed by Borrelia burgdorferi sensu stricto (32.4%), Borrelia afzelii (17.6%), and Borrelia valaisiana (5.9%). Four ticks had dual infection with Borrelia garinii and Borrelia burgdorferi sensu stricto. Two of the Borrelia-positive ticks were coinfected with Anaplasma phagocytophila.
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apodemus species mice are reservoir hosts of Borrelia garinii ospa serotype 4 in switzerland
Journal of Clinical Microbiology, 2002Co-Authors: D Huegli, Bettina Wilske, Chang Min Hu, Pierrefrancois Humair, Lise GernAbstract:Among Borrelia burgdorferi sensu lato isolates, seven outer surface protein A (OspA) serotypes have been described: serotypes 1 and 2 correspond to B. burgdorferi sensu stricto and Borrelia afzelii, respectively, and serotypes 3 to 7 correspond to Borrelia garinii. In Europe, serotype 4 has never been isolated from Ixodes ricinus ticks until recently, although this serotype has been frequently isolated from cerebrospinal fluid from patients. In Europe, B. afzelii and B. burgdorferi sensu stricto were found associated with rodents and B. garinii was found associated with birds. In this study, the reservoir role of Apodemus mice for B. garinii OspA serotype 4 was demonstrated by xenodiagnosis. Apodemus mice are the first identified reservoir hosts for B. garinii OspA serotype 4.
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transmission of Borrelia garinii ospa serotype 4 to balb c mice by ixodes ricinus ticks collected in the field
Journal of Clinical Microbiology, 2001Co-Authors: Chang Min Hu, Volker Fingerle, Bettina Wilske, Y Lobet, Lise GernAbstract:In Europe, Borrelia garinii OspA serotype 4 has been isolated from the cerebrospinal fluid of patients but, up to now, has never been identified among culture isolates from Ixodes ricinus ticks. This information raises the question of whether OspA serotype 4 is transmitted by I. ricinus in nature. In the present study, I. ricinus nymphs collected in an area of endemicity in southern Germany were allowed to feed on mice. Cultivation of ear biopsy specimens showed that six of seven B. garinii-infected mice were infected by OspA serotype 4. In contrast, very few B. garinii OspA serotype 4 organisms were isolated directly from the ticks which infected the mice; most isolates were B. afzelii. The infected mice transmitted mainly OspA serotype 4 to xenodiagnostic ticks, preferentially in combination with B. afzelii.
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Transmission of Borrelia garinii OspA serotype 4 to BALB/c mice by Ixodes ricinus ticks collected in the field.
Journal of Clinical Microbiology, 2001Co-Authors: Chang Min Hu, Volker Fingerle, Bettina Wilske, Y Lobet, Lise GernAbstract:In Europe, Borrelia garinii OspA serotype 4 has been isolated from the cerebrospinal fluid of patients but, up to now, has never been identified among culture isolates from Ixodes ricinus ticks. This information raises the question of whether OspA serotype 4 is transmitted by I. ricinus in nature. In the present study, I. ricinus nymphs collected in an area of endemicity in southern Germany were allowed to feed on mice. Cultivation of ear biopsy specimens showed that six of seven B. garinii-infected mice were infected by OspA serotype 4. In contrast, very few B. garinii OspA serotype 4 organisms were isolated directly from the ticks which infected the mice; most isolates were B. afzelii. The infected mice transmitted mainly OspA serotype 4 to xenodiagnostic ticks, preferentially in combination with B. afzelii.
Chien-ming Shih - One of the best experts on this subject based on the ideXlab platform.
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First Detection and Molecular Identification of Borrelia garinii Spirochete from Ixodes ovatus Tick Ectoparasitized on Stray Cat in Taiwan
PLOS ONE, 2014Co-Authors: Li-lian Chao, Tsung-yu Ho, Chien-ming ShihAbstract:Borrelia garinii spirochete was detected for the first time in Ixodes ovatus tick ectoparasitized on stray cat in Taiwan. The genetic identity of this detected spirochete was determined by analyzing the gene sequence amplified by genospecies-specific polymerase chain reaction assays based on the 5S–23S intergenic spacer amplicon (rrf-rrl) and outer surface protein A (ospA) genes of B. burgdorferi sensu lato. Phylogenetic relationships were analyzed by comparing the sequences of rrf-rrl and ospA genes obtained from 27 strains of Borrelia spirochetes representing six genospecies of Borrelia. Seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. Phylogenetic analysis based on rrf-rrl gene revealed that this detected spirochete (strain IO-TP-TW) was genetically affiliated to the same clade with a high homogeneous sequences (96.7 to 98.1% similarity) within the genospecies of B. garinii and can be discriminated from other genospecies of Borrelia spirochetes. Interspecies analysis based on the genetic distance values indicates a lower level ( 0.113) to the strains identified in I. ovatus collected from Japan and China. Intraspecies analysis also reveals a higher homogeneity (GD
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first detection and molecular identification of Borrelia garinii spirochete from ixodes ovatus tick ectoparasitized on stray cat in taiwan
PLOS ONE, 2014Co-Authors: Li-lian Chao, Tsung-yu Ho, Chien-ming ShihAbstract:Borrelia garinii spirochete was detected for the first time in Ixodes ovatus tick ectoparasitized on stray cat in Taiwan. The genetic identity of this detected spirochete was determined by analyzing the gene sequence amplified by genospecies-specific polymerase chain reaction assays based on the 5S–23S intergenic spacer amplicon (rrf-rrl) and outer surface protein A (ospA) genes of B. burgdorferi sensu lato. Phylogenetic relationships were analyzed by comparing the sequences of rrf-rrl and ospA genes obtained from 27 strains of Borrelia spirochetes representing six genospecies of Borrelia. Seven major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. Phylogenetic analysis based on rrf-rrl gene revealed that this detected spirochete (strain IO-TP-TW) was genetically affiliated to the same clade with a high homogeneous sequences (96.7 to 98.1% similarity) within the genospecies of B. garinii and can be discriminated from other genospecies of Borrelia spirochetes. Interspecies analysis based on the genetic distance values indicates a lower level ( 0.113) to the strains identified in I. ovatus collected from Japan and China. Intraspecies analysis also reveals a higher homogeneity (GD<0.005) between tick (strain IO-TP-TW) and human (strain Bg-PP-TW1) isolates of B. garinii in Taiwan. This study provides the first evidence of B. garinii isolated and identified in an I. ovatus tick in Asia, and the higher homogeneity of B. garinii between tick and human strain may imply the risk of human infection by I. ovatus bite.
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molecular detection and genetic identification of Borrelia garinii and Borrelia afzelii from patients presenting with a rare skin manifestation of prurigo pigmentosa in taiwan
International Journal of Infectious Diseases, 2013Co-Authors: Li-lian Chao, Chinfang Lu, Chien-ming ShihAbstract:Summary Objectives To determine the genetic identity of Borrelia spirochetes isolated from patients with an unusual skin lesion of prurigo pigmentosa (PP) in Taiwan. The causative agents responsible for human borreliosis were clarified. Methods Serum samples and skin specimens were collected from 14 patients with suspected PP and five controls. Serological testing by Western immunoblot analysis and isolation of Borrelia spirochetes from skin specimens were used to verify the Borrelia infection. Genetic identities of isolated spirochetes were determined by analyzing the gene sequences amplified by PCR assay based on the 5S ( rrf )–23S ( rrl ) intergenic spacer amplicon gene of Borrelia burgdorferi sensu lato. Results Borrelia spirochetes were isolated from skin biopsies of three patients. Serological evidence of Borrelia infection in these patients was also confirmed by elevated IgG and IgM antibodies against the major protein antigens of B. burgdorferi . Phylogenetic analysis revealed that these detected spirochetes are genetically affiliated to the genospecies of Borrelia garinii and Borrelia afzelii with high sequence homology within the genospecies of B. garinii (91.0–98.7%) and B. afzelii (97%). Conclusions This study provides the first evidence of B. garinii and B. afzelii isolated and identified in patients with PP. Whether this unusual skin lesion is a new manifestation of Lyme disease needs to be studied further.
M. Maroli - One of the best experts on this subject based on the ideXlab platform.
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Rate of infection ofIxodes ricinus ticks withBorrelia burgdorferi sensu stricto,Borrelia garinii,Borrelia afzelii and group VS116 in an endemic focus of Lyme disease in Italy
European Journal of Clinical Microbiology and Infectious Diseases, 1998Co-Authors: M. Cinco, D. Padovan, R. Murgia, L. Poldini, L. Frusteri, N. Verbeek-de Kruif, S. Rijpkema, M. MaroliAbstract:A study to evaluate the natural rate of infection of Ixodes ricinus with Borrelia burgdorferi sensu lato was carried out in an endemic focus of Lyme disease in the Trieste area in northern Italy. Two-hundred and twenty-seven ticks collected in ten different stations were tested individually for the presence of the spirochetes using polymerase chain reaction techniques able to identify both Borrelia burgdorferi sensu lato and the four genospecies ( Borrelia burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii and group VS116). Multiple infection of individual ticks was found. The infection rate ranged from 0–70%. Infection of Ixodes ricinus with Borrelia burgdorferi group VS116 was found for the first time in Italy in both a high and a low endemic focus of Lyme disease.
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rate of infection of ixodes ricinus ticks with Borrelia burgdorferi sensu stricto Borrelia garinii Borrelia afzelii and group vs116 in an endemic focus of lyme disease in italy
European Journal of Clinical Microbiology & Infectious Diseases, 1998Co-Authors: Marina Cinco, Sjoerd Rijpkema, D. Padovan, R. Murgia, L. Poldini, L. Frusteri, Verbeekde N Kruif, M. MaroliAbstract:A study to evaluate the natural rate of infection ofIxodes ricinus withBorrelia burgdorferi sensu lato was carried out in an endemic focus of Lyme disease in the Trieste area in northern Italy. Two-hundred and twenty-seven ticks collected in ten different stations were tested individually for the presence of the spirochetes using polymerase chain reaction techniques able to identify bothBorrelia burgdorferi sensu lato and the four genospecies (Borrelia burgdorferi sensu stricto,Borrelia garinii, Borrelia afzelii and group VS116). Multiple infection of individual ticks was found. The infection rate ranged from 0–70%. Infection ofIxodes ricinus withBorrelia burgdorferi group VS116 was found for the first time in Italy in both a high and a low endemic focus of Lyme disease.
