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José Maurício Simões Bento - One of the best experts on this subject based on the ideXlab platform.
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(3S,6E)-nerolidol-mediated rendezvous of Cyclocephala paraguayensis beetles in Bottle Gourd flowers.
PloS one, 2020Co-Authors: Arodi P. Favaris, Amanda C. Túler, Weliton D. Silva, Sérgio Roberto Rodrigues, Walter S. Leal, José Maurício Simões BentoAbstract:Cyclocephalini beetles of the genus Cyclocephala (Coleoptera: Melolonthidae: Dynastinae) use flowers of some plants as food, shelter, and mating sites. However, little is known about floral scent chemistry involved in this interaction. Here we show that a sesquiterpene alcohol mediates attraction of Cyclocephala paraguayensis Arrow, on Bottle Gourd flowers, Lagenaria siceraria (Cucurbitaceae). Both males and females started to aggregate on the flowers at twilight; after that, mating began and remained for the entire night. GC-FID/EAD analysis of the L. siceraria floral scent collected in the field revealed that only the major constituent of the airborne volatiles elicited electroantennographic responses on male and female antennae of C. paraguayensis. This compound was identified as (3S,6E)-nerolidol, which was tested in two field trapping trials in Brazil. In the first bioassay, traps baited with nerolidol (mix of isomers) captured significantly more adult C. paraguayensis than control traps. In the second field trial, catches in traps baited with a mixture of isomers or enantiopure nerolidol were significantly higher than captures in control traps, but the treatments did not differ significantly. Analysis from the gut content of adult C. paraguayensis showed the presence of pollen, suggesting that they also use Bottle Gourd flowers for their nourishment. Taken together, these results suggest that (3S,6E)-nerolidol plays an essential role in the reproductive behavior of C. paraguayensis by eliciting aggregation, mating, and feeding.
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(3S,6E)-Nerolidol-mediated rendezvous of Cyclocephalini beetles, Cyclocephala paraguayensis, in Bottle Gourd flowers
2020Co-Authors: Arodi P. Favaris, Amanda C. Túler, Weliton D. Silva, Sérgio Roberto Rodrigues, Walter S. Leal, José Maurício Simões BentoAbstract:Cyclocephalini beetles of genus Cyclocephala (Coleoptera: Melolonthidae: Dynastinae) use flowers of some plants as food, shelter, and mating stands. However, little is known about floral scent chemistry involved in this interaction. Here we show that a sesquiterpene alcohol mediates attraction of Cyclocephala paraguayensis Arrow on Bottle Gourd flowers, Lagenaria siceraria (Curcubitaceae). Both males and females started to aggregate on flowers at twilight; after that, mating began and remained for the entire night. The major constituent of the airborne volatiles from L. siceraria was fully characterized as (3S,6E)-nerolidol, which elicited electroantennographic responses on male and female antennae. Field bioassays showed that traps baited with the natural stereoisomer or a mix of nerolidol isomers captured significantly more males and females of C. paraguayensis than control traps. Analysis from the gut content of these Cyclocephalini beetles showed the presence of pollen, suggesting that they also use Bottle Gourd flowers for their nourishment. Taken together, these results suggest that (3S,6E)-nerolidol plays an essential role in the reproductive behavior of C. paraguayensis by eliciting aggregation, mating, and feeding.
Amnon Levi - One of the best experts on this subject based on the ideXlab platform.
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transcriptome changes in reciprocal grafts involving watermelon and Bottle Gourd reveal molecular mechanisms involved in increase of the fruit size rind toughness and soluble solids
Plant Molecular Biology, 2020Co-Authors: Marleny Garcialozano, Amnon Levi, S K Dutta, Purushothaman Natarajan, Yan R Tomason, Carlos Lopez, Ramesh Katam, Padma Nimmakayala, Umesh K ReddyAbstract:Transcriptome landscape reveals the molecular mechanisms involved in the improvement of fruit traits by the grafting of watermelon and Bottle Gourd. Grafting has been used as a sustainable alternative for watermelon breeding to control soil-borne pathogens and to increase tolerance to various abiotic stresses. However, some reports have shown that grafting can negatively affect the quality of fruits. Despite several field studies on the effects of grafting on fruit quality, the regulation of this process at the molecular level has not been revealed. The aim of this study was to elucidate various molecular mechanisms involved in different tissues of heterografted watermelon and Bottle Gourd plants. Grafting with Bottle Gourd rootstock increased the size and rind thickness of watermelon fruits, whereas that with watermelon rootstock produced Bottle Gourd fruits with higher total soluble solid content and thinner rinds. Correspondingly, genes related to ripening, softening, cell wall strengthening, stress response and disease resistance were differentially expressed in watermelon fruits. Moreover, genes associated mainly with sugar metabolism were differentially expressed in Bottle Gourd fruits. RNA-seq revealed more than 400 mobile transcripts across the heterografted sets. More than half of these were validated from PlaMoM, a database for plant mobile macromolecules. In addition, some of these mobile transcripts contained a transfer RNA-like structure. Other RNA motifs were also enriched in these transcripts, most with a biological role based on GO analysis. This transcriptome study provided a comprehensive understanding of various molecular mechanisms underlying grafted tissues in watermelon.
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the Bottle Gourd genome provides insights into cucurbitaceae evolution and facilitates mapping of a papaya ring spot virus resistance locus
Plant Journal, 2017Co-Authors: Honghe Sun, Jérôme Salse, Xuelian Sui, Alan Wilder, Amnon Levi, Kai-shu Ling, Zhangjun FeiAbstract:Summary Bottle Gourd (Lagenaria siceraria) is an important vegetable crop as well as a rootstock for other cucurbit crops. In this study, we report a high-quality 313.4-Mb genome sequence of a Bottle Gourd inbred line, USVL1VR-Ls, with a scaffold N50 of 8.7 Mb and the longest of 19.0 Mb. About 98.3% of the assembled scaffolds are anchored to the 11 pseudomolecules. Our comparative genomic analysis identifies chromosome-level syntenic relationships between Bottle Gourd and other cucurbits, as well as lineage-specific gene family expansions in Bottle Gourd. We reconstruct the genome of the most recent common ancestor of Cucurbitaceae, which reveals that the ancestral Cucurbitaceae karyotypes consists of 12 protochromosomes with 18,534 protogenes. The 12 protochromosomes are largely retained in the modern melon genome, while have undergone different degrees of shuffling events in other investigated cucurbit genomes. The eleven Bottle Gourd chromosomes derive from the ancestral Cucurbitaceae karyotypes followed by 19 chromosomal fissions and 20 fusions. The Bottle Gourd genome sequence has facilitated the mapping of a dominant monogenic locus, Prs, conferring Papaya ringspot virus (PRSV) resistance in Bottle Gourd, to a 317.8-kb region on chromosome 1. We have developed a cleaved amplified polymorphic sequence (CAPS) marker tightly linked to the Prs locus and demonstrated its potential application in marker-assisted selection of PRSV resistance in Bottle Gourd. This study provides insights into the paleohistory of Cucurbitaceae genome evolution, and the high-quality genome sequence of Bottle Gourd provides a useful resource for plant comparative genomics studies and cucurbit improvement. This article is protected by copyright. All rights reserved.
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development and field evaluation of multiple virus resistant Bottle Gourd lagenaria siceraria
Plant Disease, 2013Co-Authors: Kai-shu Ling, Amnon Levi, Scott Adkins, Chandrasekar S Kousik, G Miller, Richard L Hassell, Anthony P KeinathAbstract:Abstract In an effort to develop Bottle Gourd (Lagenaria siceraria) as a widely adapted rootstock for watermelon grafting, we sought to identify lines with broad resistance to several cucurbit viruses that are economically important in the United States. Preliminary analysis under greenhouse conditions indicated that the currently available commercial watermelon rootstocks were either highly susceptible or somewhat tolerant to one or more viruses. However, in greenhouse screening, several breeding lines of Bottle Gourd displayed broad-spectrum resistance to four viruses tested, including Zucchini yellow mosaic virus, Watermelon mosaic virus (WMV), Papaya ringspot virus watermelon strain (PRSV-W), and Squash vein yellowing virus. Resistance to PRSV-W and WMV was confirmed through field trials in two consecutive years at two different locations in South Carolina. Two breeding lines (USVL#1-8 and USVL#5-5) with broad-spectrum virus resistance could be useful materials for watermelon rootstock development.
Alfred Odindo - One of the best experts on this subject based on the ideXlab platform.
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photosynthetic response of Bottle Gourd lagenaria siceraria molina standl to drought stress relationship between cucurbitacins accumulation and drought tolerance
Scientia Horticulturae, 2018Co-Authors: Jacob Mashilo, Alfred Odindo, Hussein Shimelis, Pearl Musenge, Samson Zeray Tesfay, Lembe Samukelo MagwazaAbstract:Abstract Cucurbitacins are a group of secondary metabolites produced by cucurbits including Bottle Gourd [Lagenaria siceraria (Molina) Standl.]. The role of cucurbitacins in plant responses to drought stress is not well-determined. The objective of this study was to investigate the response of Bottle Gourd to drought stress and to establish the relationship between cucurbitacins accumulation and drought tolerance. Leaf gas exchange, chlorophyll fluorescence and level of cucurbitacins production were investigated on 12 Bottle-Gourd landraces grown under drought-stressed (DS) and non-stressed (NS) conditions. Results showed that stomatal conductance (gs), transpiration (T) rate, net CO2 assimilation rate (A), the ratio of CO2 assimilation rate and intercellular CO2 concentration (Ci/Ca), intrinsic and instantaneous water-use efficiencies declined in landraces that were subjected to drought stress. Intercellular CO2 concentration, the ratio of intercellular and atmospheric CO2 concentration increased significantly in response to drought stress. The maximum quantum efficiency of photosystem II photochemistry (Fv’/Fm’), the effective quantum efficiency of photosystem II photochemistry, photochemical quenching (qP) and non-photochemical quenching (qN) were not affected by drought stress; whereas, electron transport rate, electron transport to oxygen molecules (ETR/A) and alternative electron sink (AES) declined. Cucurbitacin E and I were detected under NS and DS conditions in some of the tested Bottle Gourd landraces. Significant and positive correlations were observed between cucurbitacin I content with ETR/A and AES. The current study identified drought tolerant Bottle Gourd landraces namely: BG-48, BG-58, BG-70, BG-78 and BG-79 based of high values for gs, T, A, Fv’/Fm’ and qN under DS condition. These selections may be useful for drought tolerance breeding in Bottle Gourd or related cucurbits. There is a need for a further investigation whether cucurbitacin I accumulation may be involved in the regulation of the physiological processes evaluated in the present study.
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drought tolerance of selected Bottle Gourd lagenaria siceraria molina standl landraces assessed by leaf gas exchange and photosynthetic efficiency
Plant Physiology and Biochemistry, 2017Co-Authors: Jacob Mashilo, Alfred Odindo, Hussein Shimelis, Pearl Musenge, Samson Zeray Tesfay, Lembe Samukelo MagwazaAbstract:Abstract Successful cultivation of Bottle Gourd in arid and semi-arid areas of sub-Saharan Africa and globally requires the identification of drought tolerant parents for developing superior genotypes with increased drought resistance. The objective of this study was to determine the level of drought tolerance among genetically diverse South African Bottle Gourd landraces based on leaf gas exchange and photosynthetic efficiency and identify promising genotypes for breeding. The responses of 12 Bottle Gourd landraces grown in glasshouse under non-stressed (NS) and drought-stressed (DS) conditions were studied. A significant genotype x water regime interaction was observed for gs, T , A , A/C i , IWUE, WUE ins , F m ′, F v ′/ F m ′, Ф PSII , qP, qN, ETR, ETR/A and AES indicating variability in response among the studied Bottle Gourd landraces under NS and DS conditions. Principal component analysis identified three principal components (PC's) under drought stress condition contributing to 82.9% of total variation among leaf gas exchange and chlorophyll fluorescence parameters measured. PC1 explained 36% of total variation contributed by gs, T , F 0 ′, F m ′, F v ′ /F m ′ and qN, while PC2 explained 28% of the variation and highly correlated with A , A/C i , IWUE, WUE ins ETR/A and AES. PC3 explained 14% of total variation contributed by Ф PSII , qP and ETR. Principal biplot analysis allowed the identification of drought tolerant genotypes such as BG-27, BG-48, BG-58, BG-79, BG-70 and BG-78 which were grouped based on high gs, A , F m ′ F v ′/ F m ′, qN, ETR/A and AES under DS condition. The study suggests that the identified physiological traits could be useful indicators in the selection of Bottle Gourd genotypes for increased drought tolerance.
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phenotypic and genotypic characterization of Bottle Gourd lagenaria siceraria molina standl and implications for breeding a review
Scientia Horticulturae, 2017Co-Authors: Jacob Mashilo, Hussein Shimelis, Alfred OdindoAbstract:Abstract Bottle Gourd [ Lagenaria siceraria (Molina) Standl.] belonging to the family Cucurbitaceae is an important crop widely grown for its fruits, seeds or succulent leaves. Sub-Saharan Africa (SSA) is believed to be the centre of genetic diversity of Bottle Gourd where it is commonly grown by smallholder farmers predominantly using unimproved and genetically diverse landrace varieties. Bottle Gourd received limited research and development priority towards genetic improvement and cultivar deployment and conservation in SSA compared to the continental Asia and America. The genetic variation present among landraces in SSA is a useful raw material for Bottle Gourd improvement programmes in the region and globally. The objective of this review is to present the perspectives on the genetic diversity and genetic resources, population structure and breeding progress of Bottle Gourd. Further, the review discusses the potential of genomic resources for genetic analysis and genomic selection of Bottle Gourd to accelerate conventional breeding in enhancing productivity and systematic conservation of the crop.
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yield based selection indices for drought tolerance evaluation in selected Bottle Gourd lagenaria siceraria molina standl landraces
Acta Agriculturae Scandinavica Section B-soil and Plant Science, 2017Co-Authors: Jacob Mashilo, Hussein Shimelis, Alfred OdindoAbstract:ABSTRACTBottle Gourd is an important crop in arid and semi-arid tropics where recurrent drought is the major constraint to crop production. Identification of drought-tolerant Bottle Gourd genotypes is fundamental to enhance productivity and for effective breeding and conservation. The objective of this study was to determine drought tolerance of a diverse set of Bottle Gourd landraces and to identify promising genotypes for direct production or breeding. A field study was conducted using a 12 × 2 factorial experiment involving 12 Bottle Gourd landraces under drought-stressed (DS) and non-stressed (NS) conditions. Significant differences were observed among Bottle Gourd landraces with respect to fruit yield under DS and NS conditions. The mean fruit yield under DS and NS conditions was 8.75 and 22.4 t ha−1, respectively. Drought stress reduced fruit yield by 62%. Correlation and principal component analyses revealed the significance of yield-based indices of drought tolerance, such as tolerance index, geom...
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correlation and path coefficient analyses of qualitative and quantitative traits in selected Bottle Gourd landraces
Acta Agriculturae Scandinavica Section B-soil and Plant Science, 2016Co-Authors: Jacob Mashilo, Hussein Shimelis, Alfred OdindoAbstract:ABSTRACTKnowledge on associations between yield and related traits is vital to improve selection efficiency in cultivar improvement. This study determined the relationships among qualitative and quantitative traits in Bottle Gourd landraces using simple correlation and path analyses. Results showed significant and positive correlations between the number of fruits per plant (NFPP) with number of male flowers (NMF), number of female flowers (NFF), plant height (PHT) and number of branches (NB). Number of seeds per fruit (NSPF) positively correlated with PHT, NMF, NFF, NB and fruit weight (FW). Fruit texture, degree of warts (DW), fruit shape (FS), and degree of neck bending, stem-end fruit shape and fruit neck length (FNL) had significant negative correlations with NFPP or NSPF. Path analysis revealed high direct path coefficient value (0.96) between FW and NSPF. Also, positive direct path coefficient value (0.92) was exhibited between NFF and NFPP. Relatively high direct path coefficient value (0.47) was ...
Jacob Mashilo - One of the best experts on this subject based on the ideXlab platform.
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photosynthetic response of Bottle Gourd lagenaria siceraria molina standl to drought stress relationship between cucurbitacins accumulation and drought tolerance
Scientia Horticulturae, 2018Co-Authors: Jacob Mashilo, Alfred Odindo, Hussein Shimelis, Pearl Musenge, Samson Zeray Tesfay, Lembe Samukelo MagwazaAbstract:Abstract Cucurbitacins are a group of secondary metabolites produced by cucurbits including Bottle Gourd [Lagenaria siceraria (Molina) Standl.]. The role of cucurbitacins in plant responses to drought stress is not well-determined. The objective of this study was to investigate the response of Bottle Gourd to drought stress and to establish the relationship between cucurbitacins accumulation and drought tolerance. Leaf gas exchange, chlorophyll fluorescence and level of cucurbitacins production were investigated on 12 Bottle-Gourd landraces grown under drought-stressed (DS) and non-stressed (NS) conditions. Results showed that stomatal conductance (gs), transpiration (T) rate, net CO2 assimilation rate (A), the ratio of CO2 assimilation rate and intercellular CO2 concentration (Ci/Ca), intrinsic and instantaneous water-use efficiencies declined in landraces that were subjected to drought stress. Intercellular CO2 concentration, the ratio of intercellular and atmospheric CO2 concentration increased significantly in response to drought stress. The maximum quantum efficiency of photosystem II photochemistry (Fv’/Fm’), the effective quantum efficiency of photosystem II photochemistry, photochemical quenching (qP) and non-photochemical quenching (qN) were not affected by drought stress; whereas, electron transport rate, electron transport to oxygen molecules (ETR/A) and alternative electron sink (AES) declined. Cucurbitacin E and I were detected under NS and DS conditions in some of the tested Bottle Gourd landraces. Significant and positive correlations were observed between cucurbitacin I content with ETR/A and AES. The current study identified drought tolerant Bottle Gourd landraces namely: BG-48, BG-58, BG-70, BG-78 and BG-79 based of high values for gs, T, A, Fv’/Fm’ and qN under DS condition. These selections may be useful for drought tolerance breeding in Bottle Gourd or related cucurbits. There is a need for a further investigation whether cucurbitacin I accumulation may be involved in the regulation of the physiological processes evaluated in the present study.
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drought tolerance of selected Bottle Gourd lagenaria siceraria molina standl landraces assessed by leaf gas exchange and photosynthetic efficiency
Plant Physiology and Biochemistry, 2017Co-Authors: Jacob Mashilo, Alfred Odindo, Hussein Shimelis, Pearl Musenge, Samson Zeray Tesfay, Lembe Samukelo MagwazaAbstract:Abstract Successful cultivation of Bottle Gourd in arid and semi-arid areas of sub-Saharan Africa and globally requires the identification of drought tolerant parents for developing superior genotypes with increased drought resistance. The objective of this study was to determine the level of drought tolerance among genetically diverse South African Bottle Gourd landraces based on leaf gas exchange and photosynthetic efficiency and identify promising genotypes for breeding. The responses of 12 Bottle Gourd landraces grown in glasshouse under non-stressed (NS) and drought-stressed (DS) conditions were studied. A significant genotype x water regime interaction was observed for gs, T , A , A/C i , IWUE, WUE ins , F m ′, F v ′/ F m ′, Ф PSII , qP, qN, ETR, ETR/A and AES indicating variability in response among the studied Bottle Gourd landraces under NS and DS conditions. Principal component analysis identified three principal components (PC's) under drought stress condition contributing to 82.9% of total variation among leaf gas exchange and chlorophyll fluorescence parameters measured. PC1 explained 36% of total variation contributed by gs, T , F 0 ′, F m ′, F v ′ /F m ′ and qN, while PC2 explained 28% of the variation and highly correlated with A , A/C i , IWUE, WUE ins ETR/A and AES. PC3 explained 14% of total variation contributed by Ф PSII , qP and ETR. Principal biplot analysis allowed the identification of drought tolerant genotypes such as BG-27, BG-48, BG-58, BG-79, BG-70 and BG-78 which were grouped based on high gs, A , F m ′ F v ′/ F m ′, qN, ETR/A and AES under DS condition. The study suggests that the identified physiological traits could be useful indicators in the selection of Bottle Gourd genotypes for increased drought tolerance.
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phenotypic and genotypic characterization of Bottle Gourd lagenaria siceraria molina standl and implications for breeding a review
Scientia Horticulturae, 2017Co-Authors: Jacob Mashilo, Hussein Shimelis, Alfred OdindoAbstract:Abstract Bottle Gourd [ Lagenaria siceraria (Molina) Standl.] belonging to the family Cucurbitaceae is an important crop widely grown for its fruits, seeds or succulent leaves. Sub-Saharan Africa (SSA) is believed to be the centre of genetic diversity of Bottle Gourd where it is commonly grown by smallholder farmers predominantly using unimproved and genetically diverse landrace varieties. Bottle Gourd received limited research and development priority towards genetic improvement and cultivar deployment and conservation in SSA compared to the continental Asia and America. The genetic variation present among landraces in SSA is a useful raw material for Bottle Gourd improvement programmes in the region and globally. The objective of this review is to present the perspectives on the genetic diversity and genetic resources, population structure and breeding progress of Bottle Gourd. Further, the review discusses the potential of genomic resources for genetic analysis and genomic selection of Bottle Gourd to accelerate conventional breeding in enhancing productivity and systematic conservation of the crop.
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yield based selection indices for drought tolerance evaluation in selected Bottle Gourd lagenaria siceraria molina standl landraces
Acta Agriculturae Scandinavica Section B-soil and Plant Science, 2017Co-Authors: Jacob Mashilo, Hussein Shimelis, Alfred OdindoAbstract:ABSTRACTBottle Gourd is an important crop in arid and semi-arid tropics where recurrent drought is the major constraint to crop production. Identification of drought-tolerant Bottle Gourd genotypes is fundamental to enhance productivity and for effective breeding and conservation. The objective of this study was to determine drought tolerance of a diverse set of Bottle Gourd landraces and to identify promising genotypes for direct production or breeding. A field study was conducted using a 12 × 2 factorial experiment involving 12 Bottle Gourd landraces under drought-stressed (DS) and non-stressed (NS) conditions. Significant differences were observed among Bottle Gourd landraces with respect to fruit yield under DS and NS conditions. The mean fruit yield under DS and NS conditions was 8.75 and 22.4 t ha−1, respectively. Drought stress reduced fruit yield by 62%. Correlation and principal component analyses revealed the significance of yield-based indices of drought tolerance, such as tolerance index, geom...
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correlation and path coefficient analyses of qualitative and quantitative traits in selected Bottle Gourd landraces
Acta Agriculturae Scandinavica Section B-soil and Plant Science, 2016Co-Authors: Jacob Mashilo, Hussein Shimelis, Alfred OdindoAbstract:ABSTRACTKnowledge on associations between yield and related traits is vital to improve selection efficiency in cultivar improvement. This study determined the relationships among qualitative and quantitative traits in Bottle Gourd landraces using simple correlation and path analyses. Results showed significant and positive correlations between the number of fruits per plant (NFPP) with number of male flowers (NMF), number of female flowers (NFF), plant height (PHT) and number of branches (NB). Number of seeds per fruit (NSPF) positively correlated with PHT, NMF, NFF, NB and fruit weight (FW). Fruit texture, degree of warts (DW), fruit shape (FS), and degree of neck bending, stem-end fruit shape and fruit neck length (FNL) had significant negative correlations with NFPP or NSPF. Path analysis revealed high direct path coefficient value (0.96) between FW and NSPF. Also, positive direct path coefficient value (0.92) was exhibited between NFF and NFPP. Relatively high direct path coefficient value (0.47) was ...
Kai-shu Ling - One of the best experts on this subject based on the ideXlab platform.
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the Bottle Gourd genome provides insights into cucurbitaceae evolution and facilitates mapping of a papaya ring spot virus resistance locus
Plant Journal, 2017Co-Authors: Honghe Sun, Jérôme Salse, Xuelian Sui, Alan Wilder, Amnon Levi, Kai-shu Ling, Zhangjun FeiAbstract:Summary Bottle Gourd (Lagenaria siceraria) is an important vegetable crop as well as a rootstock for other cucurbit crops. In this study, we report a high-quality 313.4-Mb genome sequence of a Bottle Gourd inbred line, USVL1VR-Ls, with a scaffold N50 of 8.7 Mb and the longest of 19.0 Mb. About 98.3% of the assembled scaffolds are anchored to the 11 pseudomolecules. Our comparative genomic analysis identifies chromosome-level syntenic relationships between Bottle Gourd and other cucurbits, as well as lineage-specific gene family expansions in Bottle Gourd. We reconstruct the genome of the most recent common ancestor of Cucurbitaceae, which reveals that the ancestral Cucurbitaceae karyotypes consists of 12 protochromosomes with 18,534 protogenes. The 12 protochromosomes are largely retained in the modern melon genome, while have undergone different degrees of shuffling events in other investigated cucurbit genomes. The eleven Bottle Gourd chromosomes derive from the ancestral Cucurbitaceae karyotypes followed by 19 chromosomal fissions and 20 fusions. The Bottle Gourd genome sequence has facilitated the mapping of a dominant monogenic locus, Prs, conferring Papaya ringspot virus (PRSV) resistance in Bottle Gourd, to a 317.8-kb region on chromosome 1. We have developed a cleaved amplified polymorphic sequence (CAPS) marker tightly linked to the Prs locus and demonstrated its potential application in marker-assisted selection of PRSV resistance in Bottle Gourd. This study provides insights into the paleohistory of Cucurbitaceae genome evolution, and the high-quality genome sequence of Bottle Gourd provides a useful resource for plant comparative genomics studies and cucurbit improvement. This article is protected by copyright. All rights reserved.
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development and field evaluation of multiple virus resistant Bottle Gourd lagenaria siceraria
Plant Disease, 2013Co-Authors: Kai-shu Ling, Amnon Levi, Scott Adkins, Chandrasekar S Kousik, G Miller, Richard L Hassell, Anthony P KeinathAbstract:Abstract In an effort to develop Bottle Gourd (Lagenaria siceraria) as a widely adapted rootstock for watermelon grafting, we sought to identify lines with broad resistance to several cucurbit viruses that are economically important in the United States. Preliminary analysis under greenhouse conditions indicated that the currently available commercial watermelon rootstocks were either highly susceptible or somewhat tolerant to one or more viruses. However, in greenhouse screening, several breeding lines of Bottle Gourd displayed broad-spectrum resistance to four viruses tested, including Zucchini yellow mosaic virus, Watermelon mosaic virus (WMV), Papaya ringspot virus watermelon strain (PRSV-W), and Squash vein yellowing virus. Resistance to PRSV-W and WMV was confirmed through field trials in two consecutive years at two different locations in South Carolina. Two breeding lines (USVL#1-8 and USVL#5-5) with broad-spectrum virus resistance could be useful materials for watermelon rootstock development.