The Experts below are selected from a list of 198 Experts worldwide ranked by ideXlab platform
Cristina Pujades - One of the best experts on this subject based on the ideXlab platform.
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Yap/Taz-TEAD activity links mechanical cues to progenitor Cell behavior during zebrafish hindbrain segmentation.
Development (Cambridge England), 2019Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Carolyn Engel-pizcueta, Cristina PujadesAbstract:ABSTRACT Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific Cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, and to Cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that Boundary Cells responded to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their Cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain Boundary Cell population.
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yap taz tead activity links mechanical cues to progenitor Cell behavior during zebrafish hindbrain segmentation
Development, 2019Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Carolyn Engelpizcueta, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:ABSTRACT Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific Cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, and to Cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that Boundary Cells responded to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their Cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain Boundary Cell population.
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yap taz tead activity links mechanical cues to specific Cell fate within the hindbrain boundaries
bioRxiv, 2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:How embryonic Cells perceive their microenvironment through physical/mechanical cues during morphogenesis remains largely unexplored. The YAP/TAZ family has emerged as a fundamentally important regulator of Cell proliferation, responding to cues from the extraCellular matrix, Cell shape and the actomyosin cytoskeleton. However, how signals are interpreted during embryonic tissue deformation resulting in specific Cell fates has not been solved yet. In this work, we use the zebrafish hindbrain to explore how changes in tissue architecture during tissue segmentation affect gene expression and thereby ultimately inform Cell decisions. We unveil the role of Yap/Taz-TEAD activity in hindbrain boundaries as sensor and effector of mechanical signals in the regulation of Cell fate upon hindbrain compartmentalization, and show that Boundary Cells respond to mechanical cues Cell-autonomously through Yap/Taz-TEAD-activity. Further, Cell-lineage analysis reveals that Yap/Taz-TEAD Boundary Cells display heterochronic proliferative capacity, and this switch in Cell proliferation results in Cell fate changes, from proliferating progenitors to differentiated neurons. Finally, we demonstrate the role of Yap/Taz-TEAD activity in maintaining the Cell progenitor features in the hindbrain Boundary Cell population.
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YAP/TAZ-TEAD Activity Links Mechanical Cues To Specific Cell Fate Within The Hindbrain Boundaries
2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:How embryonic Cells perceive their microenvironment through physical/mechanical cues during morphogenesis remains largely unexplored. The YAP/TAZ family has emerged as a fundamentally important regulator of Cell proliferation, responding to cues from the extraCellular matrix, Cell shape and the actomyosin cytoskeleton. However, how signals are interpreted during embryonic tissue deformation resulting in specific Cell fates has not been solved yet. In this work, we use the zebrafish hindbrain to explore how changes in tissue architecture during tissue segmentation affect gene expression and thereby ultimately inform Cell decisions. We unveil the role of Yap/Taz-TEAD activity in hindbrain boundaries as sensor and effector of mechanical signals in the regulation of Cell fate upon hindbrain compartmentalization, and show that Boundary Cells respond to mechanical cues Cell-autonomously through Yap/Taz-TEAD-activity. Further, Cell-lineage analysis reveals that Yap/Taz-TEAD Boundary Cells display heterochronic proliferative capacity, and this switch in Cell proliferation results in Cell fate changes, from proliferating progenitors to differentiated neurons. Finally, we demonstrate the role of Yap/Taz-TEAD activity in maintaining the Cell progenitor features in the hindbrain Boundary Cell population.
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YAP/TAZ-TEAD Activity Links Mechanical Cues To Cell Progenitor Behavior During Hindbrain Segmentation
2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:SUMMARY Cells perceive their microenvironment through chemical and physical cues. However, how mechanical signals are interpreted during embryonic tissue deformation resulting in specific Cell behaviors is largely unexplored. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, Cell shape and actomyosin cytoskeleton. In this work, we unveiled the role of Yap/Taz-TEAD activity as sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during hindbrain compartmentalization. Monitoring in vivo Yap/Taz-activity during hindbrain segmentation we discovered that Boundary Cells respond to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decrease their proliferative activity when Yap/Taz-TEAD ceased, preceding changes of Cell fate: from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining the progenitor features in the hindbrain Boundary Cell population.
Javier Terriente - One of the best experts on this subject based on the ideXlab platform.
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Yap/Taz-TEAD activity links mechanical cues to progenitor Cell behavior during zebrafish hindbrain segmentation.
Development (Cambridge England), 2019Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Carolyn Engel-pizcueta, Cristina PujadesAbstract:ABSTRACT Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific Cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, and to Cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that Boundary Cells responded to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their Cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain Boundary Cell population.
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yap taz tead activity links mechanical cues to progenitor Cell behavior during zebrafish hindbrain segmentation
Development, 2019Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Carolyn Engelpizcueta, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:ABSTRACT Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific Cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, and to Cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that Boundary Cells responded to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their Cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain Boundary Cell population.
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yap taz tead activity links mechanical cues to specific Cell fate within the hindbrain boundaries
bioRxiv, 2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:How embryonic Cells perceive their microenvironment through physical/mechanical cues during morphogenesis remains largely unexplored. The YAP/TAZ family has emerged as a fundamentally important regulator of Cell proliferation, responding to cues from the extraCellular matrix, Cell shape and the actomyosin cytoskeleton. However, how signals are interpreted during embryonic tissue deformation resulting in specific Cell fates has not been solved yet. In this work, we use the zebrafish hindbrain to explore how changes in tissue architecture during tissue segmentation affect gene expression and thereby ultimately inform Cell decisions. We unveil the role of Yap/Taz-TEAD activity in hindbrain boundaries as sensor and effector of mechanical signals in the regulation of Cell fate upon hindbrain compartmentalization, and show that Boundary Cells respond to mechanical cues Cell-autonomously through Yap/Taz-TEAD-activity. Further, Cell-lineage analysis reveals that Yap/Taz-TEAD Boundary Cells display heterochronic proliferative capacity, and this switch in Cell proliferation results in Cell fate changes, from proliferating progenitors to differentiated neurons. Finally, we demonstrate the role of Yap/Taz-TEAD activity in maintaining the Cell progenitor features in the hindbrain Boundary Cell population.
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YAP/TAZ-TEAD Activity Links Mechanical Cues To Specific Cell Fate Within The Hindbrain Boundaries
2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:How embryonic Cells perceive their microenvironment through physical/mechanical cues during morphogenesis remains largely unexplored. The YAP/TAZ family has emerged as a fundamentally important regulator of Cell proliferation, responding to cues from the extraCellular matrix, Cell shape and the actomyosin cytoskeleton. However, how signals are interpreted during embryonic tissue deformation resulting in specific Cell fates has not been solved yet. In this work, we use the zebrafish hindbrain to explore how changes in tissue architecture during tissue segmentation affect gene expression and thereby ultimately inform Cell decisions. We unveil the role of Yap/Taz-TEAD activity in hindbrain boundaries as sensor and effector of mechanical signals in the regulation of Cell fate upon hindbrain compartmentalization, and show that Boundary Cells respond to mechanical cues Cell-autonomously through Yap/Taz-TEAD-activity. Further, Cell-lineage analysis reveals that Yap/Taz-TEAD Boundary Cells display heterochronic proliferative capacity, and this switch in Cell proliferation results in Cell fate changes, from proliferating progenitors to differentiated neurons. Finally, we demonstrate the role of Yap/Taz-TEAD activity in maintaining the Cell progenitor features in the hindbrain Boundary Cell population.
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YAP/TAZ-TEAD Activity Links Mechanical Cues To Cell Progenitor Behavior During Hindbrain Segmentation
2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:SUMMARY Cells perceive their microenvironment through chemical and physical cues. However, how mechanical signals are interpreted during embryonic tissue deformation resulting in specific Cell behaviors is largely unexplored. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, Cell shape and actomyosin cytoskeleton. In this work, we unveiled the role of Yap/Taz-TEAD activity as sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during hindbrain compartmentalization. Monitoring in vivo Yap/Taz-activity during hindbrain segmentation we discovered that Boundary Cells respond to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decrease their proliferative activity when Yap/Taz-TEAD ceased, preceding changes of Cell fate: from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining the progenitor features in the hindbrain Boundary Cell population.
David G. Wilkinson - One of the best experts on this subject based on the ideXlab platform.
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Actomyosin regulation by Eph receptor signaling couples Boundary Cell formation to border sharpness.
eLife, 2019Co-Authors: Jordi Cayuso, Megan Addison, David G. WilkinsonAbstract:The segregation of Cells with distinct regional identity underlies formation of a sharp border, which in some tissues serves to organise a Boundary signaling centre. It is unclear whether or how border sharpness is coordinated with induction of Boundary-specific gene expression. We show that forward signaling of EphA4 is required for border sharpening and induction of Boundary Cells in the zebrafish hindbrain, which we find both require kinase-dependent signaling, with a lesser input of PDZ domain-dependent signaling. We find that Boundary-specific gene expression is regulated by myosin II phosphorylation, which increases actomyosin contraction downstream of EphA4 signaling. Myosin phosphorylation leads to nuclear translocation of Taz, which together with Tead1a is required for Boundary marker expression. Since actomyosin contraction maintains sharp borders, there is direct coupling of border sharpness to Boundary Cell induction that ensures correct organisation of signaling centres.
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Signalling from hindbrain boundaries regulates neuronal clustering that patterns neurogenesis
Development (Cambridge England), 2012Co-Authors: Javier Terriente, Sebastian S. Gerety, Tomomi Watanabe-asaka, Rosa Gonzalez-quevedo, David G. WilkinsonAbstract:During central nervous system development, neural progenitors are patterned to form discrete neurogenic and non-neurogenic zones. In the zebrafish hindbrain, neurogenesis is organised by Fgf20a emanating from neurons located at each segment centre that inhibits neuronal differentiation in adjacent progenitors. Here, we have identified a molecular mechanism that clusters fgf20a-expressing neurons in segment centres and uncovered a requirement for this positioning in the regulation of neurogenesis. Disruption of hindbrain Boundary Cell formation alters the organisation of fgf20a-expressing neurons, consistent with a role of chemorepulsion from boundaries. The semaphorins Sema3fb and Sema3gb, which are expressed by Boundary Cells, and their receptor Nrp2a are required for clustering of fgf20a-expressing neurons at segment centres. The dispersal of fgf20a-expressing neurons that occurs following the disruption of boundaries or of Sema3fb/Sema3gb signalling leads to reduced FGF target gene expression in progenitors and an increased number of differentiating neurons. Sema3 signalling from boundaries thus links hindbrain segmentation to the positioning of fgf20a-expressing neurons that regulates neurogenesis.
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morpholino artifacts provide pitfalls and reveal a novel role for pro apoptotic genes in hindbrain Boundary development
Developmental Biology, 2011Co-Authors: Sebastian S. Gerety, David G. WilkinsonAbstract:Morpholino antisense oligonucleotides (MOs) are widely used as a tool to achieve loss of gene function, but many have off-target effects mediated by activation of Tp53 and associated apoptosis. Here, we re-examine our previous MO-based loss-of-function studies that had suggested that Wnt1 expressed at hindbrain boundaries in zebrafish promotes neurogenesis and inhibits Boundary marker gene expression in the adjacent para-Boundary regions. We find that Tp53 is highly activated and apoptosis is frequently induced by the MOs used in these studies. Co-knockdown of Tp53 rescues the decrease in proneural and neuronal marker expression, which is thus an off-target effect of MOs. While loss of gene expression can be attributed to Cell loss through apoptotic Cell death, surprisingly we find that the ectopic expression of hindbrain Boundary markers is also dependent on Tp53 activity and its downstream apoptotic effectors. We examine whether this non-specific activation of hindbrain Boundary gene expression provides insight into the endogenous mechanisms underlying Boundary Cell specification. We find that the pro-apoptotic Bcl genes puma and bax-a are required for hindbrain Boundary marker expression, and that gain of function of the Bcl-caspase pathway leads to ectopic Boundary marker expression. These data reveal a non-apoptotic role for pro-apoptotic genes in the regulation of gene expression at hindbrain boundaries. In light of these findings, we discuss the precautions needed in performing morpholino knockdowns and in interpreting the data derived from their use.
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Wnt1 regulates neurogenesis and mediates lateral inhibition of Boundary Cell specification in the zebrafish hindbrain
Development, 2005Co-Authors: Marc Amoyel, Yi-chuan Cheng, Yun-jin Jiang, David G. WilkinsonAbstract:The formation of localised signalling centres is essential for patterning of a number of tissues during development. Previous work has revealed that a distinct population of Boundary Cells forms at the interface of segments in the vertebrate hindbrain, but the role of these Cells is not known. We have investigated the function of the Wnt1 signalling molecule that is expressed by Boundary and roof plate Cells in the zebrafish hindbrain. Knockdown of wnt1 or of tcf3b, a mediator of Wnt signalling, leads to ectopic expression of Boundary Cell markers, rfng and foxb1.2, in non-Boundary regions of the hindbrain. Ectopic Boundary marker expression also occurs following knockdown of rfng, a modulator of Notch signalling required for wnt1 expression at hindbrain boundaries. We show that the Boundary and roof plate expression of wnt1 each contribute to upregulation of proneural and delta gene expression and neurogenesis in non-Boundary regions, which in turn blocks ectopic Boundary marker expression. Boundary Cells therefore play a key role in the regulation of Cell differentiation in the zebrafish hindbrain. The network of genes underlying the regulation of neurogenesis and lateral inhibition of Boundary Cell formation by Wnt1 has a striking similarity to mechanisms at the dorsoventral Boundary in the Drosophila wing imaginal disc.
Adria Voltes - One of the best experts on this subject based on the ideXlab platform.
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Yap/Taz-TEAD activity links mechanical cues to progenitor Cell behavior during zebrafish hindbrain segmentation.
Development (Cambridge England), 2019Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Carolyn Engel-pizcueta, Cristina PujadesAbstract:ABSTRACT Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific Cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, and to Cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that Boundary Cells responded to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their Cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain Boundary Cell population.
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yap taz tead activity links mechanical cues to progenitor Cell behavior during zebrafish hindbrain segmentation
Development, 2019Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Carolyn Engelpizcueta, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:ABSTRACT Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific Cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, and to Cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that Boundary Cells responded to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their Cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain Boundary Cell population.
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yap taz tead activity links mechanical cues to specific Cell fate within the hindbrain boundaries
bioRxiv, 2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:How embryonic Cells perceive their microenvironment through physical/mechanical cues during morphogenesis remains largely unexplored. The YAP/TAZ family has emerged as a fundamentally important regulator of Cell proliferation, responding to cues from the extraCellular matrix, Cell shape and the actomyosin cytoskeleton. However, how signals are interpreted during embryonic tissue deformation resulting in specific Cell fates has not been solved yet. In this work, we use the zebrafish hindbrain to explore how changes in tissue architecture during tissue segmentation affect gene expression and thereby ultimately inform Cell decisions. We unveil the role of Yap/Taz-TEAD activity in hindbrain boundaries as sensor and effector of mechanical signals in the regulation of Cell fate upon hindbrain compartmentalization, and show that Boundary Cells respond to mechanical cues Cell-autonomously through Yap/Taz-TEAD-activity. Further, Cell-lineage analysis reveals that Yap/Taz-TEAD Boundary Cells display heterochronic proliferative capacity, and this switch in Cell proliferation results in Cell fate changes, from proliferating progenitors to differentiated neurons. Finally, we demonstrate the role of Yap/Taz-TEAD activity in maintaining the Cell progenitor features in the hindbrain Boundary Cell population.
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YAP/TAZ-TEAD Activity Links Mechanical Cues To Specific Cell Fate Within The Hindbrain Boundaries
2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:How embryonic Cells perceive their microenvironment through physical/mechanical cues during morphogenesis remains largely unexplored. The YAP/TAZ family has emerged as a fundamentally important regulator of Cell proliferation, responding to cues from the extraCellular matrix, Cell shape and the actomyosin cytoskeleton. However, how signals are interpreted during embryonic tissue deformation resulting in specific Cell fates has not been solved yet. In this work, we use the zebrafish hindbrain to explore how changes in tissue architecture during tissue segmentation affect gene expression and thereby ultimately inform Cell decisions. We unveil the role of Yap/Taz-TEAD activity in hindbrain boundaries as sensor and effector of mechanical signals in the regulation of Cell fate upon hindbrain compartmentalization, and show that Boundary Cells respond to mechanical cues Cell-autonomously through Yap/Taz-TEAD-activity. Further, Cell-lineage analysis reveals that Yap/Taz-TEAD Boundary Cells display heterochronic proliferative capacity, and this switch in Cell proliferation results in Cell fate changes, from proliferating progenitors to differentiated neurons. Finally, we demonstrate the role of Yap/Taz-TEAD activity in maintaining the Cell progenitor features in the hindbrain Boundary Cell population.
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YAP/TAZ-TEAD Activity Links Mechanical Cues To Cell Progenitor Behavior During Hindbrain Segmentation
2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:SUMMARY Cells perceive their microenvironment through chemical and physical cues. However, how mechanical signals are interpreted during embryonic tissue deformation resulting in specific Cell behaviors is largely unexplored. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, Cell shape and actomyosin cytoskeleton. In this work, we unveiled the role of Yap/Taz-TEAD activity as sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during hindbrain compartmentalization. Monitoring in vivo Yap/Taz-activity during hindbrain segmentation we discovered that Boundary Cells respond to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decrease their proliferative activity when Yap/Taz-TEAD ceased, preceding changes of Cell fate: from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining the progenitor features in the hindbrain Boundary Cell population.
Chaitanya Dingare - One of the best experts on this subject based on the ideXlab platform.
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Yap/Taz-TEAD activity links mechanical cues to progenitor Cell behavior during zebrafish hindbrain segmentation.
Development (Cambridge England), 2019Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Carolyn Engel-pizcueta, Cristina PujadesAbstract:ABSTRACT Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific Cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, and to Cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that Boundary Cells responded to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their Cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain Boundary Cell population.
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yap taz tead activity links mechanical cues to progenitor Cell behavior during zebrafish hindbrain segmentation
Development, 2019Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Carolyn Engelpizcueta, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:ABSTRACT Cells perceive their microenvironment through chemical and physical cues. However, how the mechanical signals are interpreted during embryonic tissue deformation to result in specific Cell behaviors is largely unknown. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, and to Cell shape and actomyosin cytoskeletal changes. In this study, we demonstrate the role of Yap/Taz-TEAD activity as a sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during zebrafish hindbrain compartmentalization. Monitoring of in vivo Yap/Taz activity during hindbrain segmentation indicated that Boundary Cells responded to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decreased their proliferative activity when Yap/Taz-TEAD activity ceased, which preceded changes in their Cell fate from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining progenitor features in the hindbrain Boundary Cell population.
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yap taz tead activity links mechanical cues to specific Cell fate within the hindbrain boundaries
bioRxiv, 2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:How embryonic Cells perceive their microenvironment through physical/mechanical cues during morphogenesis remains largely unexplored. The YAP/TAZ family has emerged as a fundamentally important regulator of Cell proliferation, responding to cues from the extraCellular matrix, Cell shape and the actomyosin cytoskeleton. However, how signals are interpreted during embryonic tissue deformation resulting in specific Cell fates has not been solved yet. In this work, we use the zebrafish hindbrain to explore how changes in tissue architecture during tissue segmentation affect gene expression and thereby ultimately inform Cell decisions. We unveil the role of Yap/Taz-TEAD activity in hindbrain boundaries as sensor and effector of mechanical signals in the regulation of Cell fate upon hindbrain compartmentalization, and show that Boundary Cells respond to mechanical cues Cell-autonomously through Yap/Taz-TEAD-activity. Further, Cell-lineage analysis reveals that Yap/Taz-TEAD Boundary Cells display heterochronic proliferative capacity, and this switch in Cell proliferation results in Cell fate changes, from proliferating progenitors to differentiated neurons. Finally, we demonstrate the role of Yap/Taz-TEAD activity in maintaining the Cell progenitor features in the hindbrain Boundary Cell population.
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YAP/TAZ-TEAD Activity Links Mechanical Cues To Specific Cell Fate Within The Hindbrain Boundaries
2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:How embryonic Cells perceive their microenvironment through physical/mechanical cues during morphogenesis remains largely unexplored. The YAP/TAZ family has emerged as a fundamentally important regulator of Cell proliferation, responding to cues from the extraCellular matrix, Cell shape and the actomyosin cytoskeleton. However, how signals are interpreted during embryonic tissue deformation resulting in specific Cell fates has not been solved yet. In this work, we use the zebrafish hindbrain to explore how changes in tissue architecture during tissue segmentation affect gene expression and thereby ultimately inform Cell decisions. We unveil the role of Yap/Taz-TEAD activity in hindbrain boundaries as sensor and effector of mechanical signals in the regulation of Cell fate upon hindbrain compartmentalization, and show that Boundary Cells respond to mechanical cues Cell-autonomously through Yap/Taz-TEAD-activity. Further, Cell-lineage analysis reveals that Yap/Taz-TEAD Boundary Cells display heterochronic proliferative capacity, and this switch in Cell proliferation results in Cell fate changes, from proliferating progenitors to differentiated neurons. Finally, we demonstrate the role of Yap/Taz-TEAD activity in maintaining the Cell progenitor features in the hindbrain Boundary Cell population.
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YAP/TAZ-TEAD Activity Links Mechanical Cues To Cell Progenitor Behavior During Hindbrain Segmentation
2018Co-Authors: Adria Voltes, Javier Terriente, Covadonga F Hevia, Chaitanya Dingare, Simone Calzolari, Caren Norden, Virginie Lecaudey, Cristina PujadesAbstract:SUMMARY Cells perceive their microenvironment through chemical and physical cues. However, how mechanical signals are interpreted during embryonic tissue deformation resulting in specific Cell behaviors is largely unexplored. The Yap/Taz family of transcriptional co-activators has emerged as an important regulator of tissue growth and regeneration, responding to physical cues from the extraCellular matrix, Cell shape and actomyosin cytoskeleton. In this work, we unveiled the role of Yap/Taz-TEAD activity as sensor of mechanical signals in the regulation of the progenitor behavior of Boundary Cells during hindbrain compartmentalization. Monitoring in vivo Yap/Taz-activity during hindbrain segmentation we discovered that Boundary Cells respond to mechanical cues in a Cell-autonomous manner through Yap/Taz-TEAD activity. Cell-lineage analysis revealed that Yap/Taz-TEAD Boundary Cells decrease their proliferative activity when Yap/Taz-TEAD ceased, preceding changes of Cell fate: from proliferating progenitors to differentiated neurons. Functional experiments demonstrated the pivotal role of Yap/Taz-TEAD signaling in maintaining the progenitor features in the hindbrain Boundary Cell population.