The Experts below are selected from a list of 1464 Experts worldwide ranked by ideXlab platform

William I. Lutterschmidt - One of the best experts on this subject based on the ideXlab platform.

  • effects of urbanization on genetic diversity gene flow and population structure in the ornate Box Turtle terrapene ornata
    Amphibia-reptilia, 2014
    Co-Authors: James C Cureton, Christopher P. Randle, William I. Lutterschmidt, Michael W Janis, Donald C Ruthven, Raelynn Deaton
    Abstract:

    Large-scale declines in North American Box Turtle (Terrapene spp.) populations have been attributed to habitat fragmentation as a result of urbanization. We compared microsatellite markers and mitochondrial control region sequences of Ornate Box Turtles (Terrapene ornata) in two populations (a natural and an urban habitat) to test two hypotheses. We hypothesized that urban populations of T. ornata experience genetic bottlenecks due to road mortality and habitat fragmentation, and that roadways represent a barrier to gene flow among Turtle populations, resulting in increased fragmentation of gene pools. Both populations shared similar allelic diversity and observed heterozygosity, with eight and seven of twelve microsatellite loci exhibiting heterozygote deficiency in the natural and urban populations, respectively. The number of mitochondrial control region haplotypes in the urban population was nearly four times that of the natural population, although only one haplotype occurred in appreciable frequency in both populations. We did not detect conclusive evidence of a recent genetic bottleneck in the urban population, thereby rejecting our first hypothesis. We detected weak differentiation among populations on opposing sides of a large highway, but did not detect any evidence of population structure, thereby rejecting our second hypothesis. This study indicates that a population of T. ornata with moderate road mortality currently has high genetic diversity, moderate inbreeding, and displays some evidence of genetic differentiation, but no conclusive evidence of recent genetic bottlenecks or unique genetic clustering. We suggest this is primarily due to the species long generation time and is a positive aspect of their life-history.

  • Isolation and characterization of polymorphic microsatellite loci for the three-toed Box Turtle (Terrapene carolina triunguis) and cross-amplification in other Terrapene species.
    Molecular ecology resources, 2009
    Co-Authors: A. B. Buchman, Raelynn Deaton, Christopher P. Randle, T. Brummel, E. D. Wilson, William I. Lutterschmidt
    Abstract:

    We isolated and characterized eight polymorphic microsatellite loci for a Texas population of three-toed Box Turtle, Terrapene carolina triunguis, using a refined hybridization capture procedure. All eight primer pairs amplified successfully at all loci in seven Texas ornate Box Turtles (T. ornata ornata). Due to the decline and conservation concerns of North American Box Turtles, these isolated microsatellites may be a most valuable tool for evaluating baseline population genetic structure for threatened Box Turtle populations.

János Gál - One of the best experts on this subject based on the ideXlab platform.

  • Adenovirus and mycoplasma infection in an ornate Box Turtle () in Hungary
    Veterinary Microbiology, 2009
    Co-Authors: Szilvia L. Farkas, János Gál
    Abstract:

    A female, adult ornate Box Turtle () with fatty liver was submitted for virologic examination in Hungary. Signs of an adenovirus infection including degeneration of the liver cells, enlarged nuclei and intranuclear inclusion bodies were detected by light microscopic examination. The presence of an adenovirus was later confirmed by obtaining partial sequence data from the adenoviral DNA-dependent DNA-polymerase. Phylogenetic analyses revealed that this novel chelonian adenovirus was distinct from previously described reptilian adenoviruses, not belonging to any of the recognized genera of the family . As a part of the routine diagnostic procedure for chelonians the detection of herpes-, rana- and iridoviruses together with spp. was attempted. Amplicons were generated by a general mycoplasma polymerase chain reaction (PCR) targeting the 16S/23S ribosomal RNA (rRNA) intergenic spacer region, as well as, a specific PCR targeting the 16S rRNA gene. Based on the analyses of partial sequences of the 16S rRNA gene, the sp. of the ornate Box Turtle seemed to be identical with the recently described eastern Box Turtle () sp. This is the first report of a novel chelonian adenovirus and a mycoplasma infection in an ornate Box Turtle () in Europe.

  • Adenovirus and mycoplasma infection in an ornate Box Turtle (Terrapene ornata ornata) in Hungary.
    Veterinary microbiology, 2009
    Co-Authors: Szilvia L. Farkas, János Gál
    Abstract:

    A female, adult ornate Box Turtle (Terrapene ornata ornata) with fatty liver was submitted for virologic examination in Hungary. Signs of an adenovirus infection including degeneration of the liver cells, enlarged nuclei and intranuclear inclusion bodies were detected by light microscopic examination. The presence of an adenovirus was later confirmed by obtaining partial sequence data from the adenoviral DNA-dependent DNA-polymerase. Phylogenetic analyses revealed that this novel chelonian adenovirus was distinct from previously described reptilian adenoviruses, not belonging to any of the recognized genera of the family Adenoviridae. As a part of the routine diagnostic procedure for chelonians the detection of herpes-, rana- and iridoviruses together with Mycoplasma spp. was attempted. Amplicons were generated by a general mycoplasma polymerase chain reaction (PCR) targeting the 16S/23S ribosomal RNA (rRNA) intergenic spacer region, as well as, a specific Mycoplasma agassizii PCR targeting the 16S rRNA gene. Based on the analyses of partial sequences of the 16S rRNA gene, the Mycoplasma sp. of the ornate Box Turtle seemed to be identical with the recently described eastern Box Turtle (Terrapene carolina carolina) Mycoplasma sp. This is the first report of a novel chelonian adenovirus and a mycoplasma infection in an ornate Box Turtle (T. ornata ornata) in Europe.

Bryan L Stuart - One of the best experts on this subject based on the ideXlab platform.

  • occurrence of the endangered keeled Box Turtle cuora mouhotii in southern vietnam
    Chelonian Conservation and Biology, 2013
    Co-Authors: Huy Duc Hoang, Bryan L Stuart
    Abstract:

    Abstract The keeled Box Turtle, Cuora mouhotii, is documented in southern Vietnam for the first time, extending the range of the species approximately 300 km southward. The southern population is morphologically similar to the subspecies C. m. obsti and occurs in sympatry with Cuora picturata, a species recently discovered in the wild for the first time. As C. mouhotii is known to hybridize with the closest living relatives of C. picturata (Cuora galbinifrons and Cuora bourreti), this finding allows for the possibility, on the basis of geography, that C. mouhotii and C. picturata may also hybridize in the wild.

  • molecular phylogeny of the critically endangered indochinese Box Turtle cuora galbinifrons
    Molecular Phylogenetics and Evolution, 2004
    Co-Authors: Bryan L Stuart, James F Parham
    Abstract:

    The Indochinese Box Turtle Cuora galbinifrons is a polytypic, critically endangered species from Vietnam, Laos, and Hainan Island, China. We analyze up to 1790bp of mitochondrial DNA under maximum parsimony and maximum likelihood criteria to test if the five historically recognized subspecies represent evolutionary lineages, and to elucidate the relationship of C. galbinifrons to other Cuora. C. galbinifrons is composed of three major mitochondrial DNA clades corresponding to the three subspecies galbinifrons, bourreti, and picturata. These three lineages are also morphologically diagnosable, and consequently we recommend elevating each to full species. Cuora galbinifrons hainanensis nests within the galbinifrons clade, and we retain it as a synonym of galbinifrons, as supported by morphology. Cuora "serrata" is known to be a hybrid of male Cuora mouhotii and female C. galbinifrons, and our findings show that C. "serrata" originates from both female galbinifrons and bourreti. Little or no mitochondrial DNA variation was found among the morphologically distinct species Cuora aurocapitata, Cuora pani, and Cuora trifasciata, for which hypotheses are proposed. Recognizing galbinifrons, bourreti, and picturata as separate species has consequences for ongoing ex situ captive breeding programs and prioritization of in situ conservation activities, particularly in Vietnam.

James F Parham - One of the best experts on this subject based on the ideXlab platform.

  • molecular phylogeny of the critically endangered indochinese Box Turtle cuora galbinifrons
    Molecular Phylogenetics and Evolution, 2004
    Co-Authors: Bryan L Stuart, James F Parham
    Abstract:

    The Indochinese Box Turtle Cuora galbinifrons is a polytypic, critically endangered species from Vietnam, Laos, and Hainan Island, China. We analyze up to 1790bp of mitochondrial DNA under maximum parsimony and maximum likelihood criteria to test if the five historically recognized subspecies represent evolutionary lineages, and to elucidate the relationship of C. galbinifrons to other Cuora. C. galbinifrons is composed of three major mitochondrial DNA clades corresponding to the three subspecies galbinifrons, bourreti, and picturata. These three lineages are also morphologically diagnosable, and consequently we recommend elevating each to full species. Cuora galbinifrons hainanensis nests within the galbinifrons clade, and we retain it as a synonym of galbinifrons, as supported by morphology. Cuora "serrata" is known to be a hybrid of male Cuora mouhotii and female C. galbinifrons, and our findings show that C. "serrata" originates from both female galbinifrons and bourreti. Little or no mitochondrial DNA variation was found among the morphologically distinct species Cuora aurocapitata, Cuora pani, and Cuora trifasciata, for which hypotheses are proposed. Recognizing galbinifrons, bourreti, and picturata as separate species has consequences for ongoing ex situ captive breeding programs and prioritization of in situ conservation activities, particularly in Vietnam.

A. B. Buchman - One of the best experts on this subject based on the ideXlab platform.