The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Rosalind E. Hand - One of the best experts on this subject based on the ideXlab platform.
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studies on triploid oysters in australia xiii performance of diploid and triploid sydney rock oyster saccostrea glomerata gould 1850 progeny from a third generation Breeding Line
Aquaculture, 2004Co-Authors: Rosalind E. Hand, John A. Nell, Peter A ThompsonAbstract:The performance of diploid and triploid selected and control oysters was compared at three sites to determine if the improvements in growth of selected oyster Line (L2) Sydney rock oysters were additive to the faster growth of triploids. After a grow-out period of 32 months, both mean whole weights and shell heights were in the order: L2 triploids>control triploids>L2 diploids>control diploids. Mean whole weights of different oyster Lines were all significantly different (p<0.05). A significant (p=0.02) site×Line interaction effect on oyster weights was also detected. On average, L2 triploids were 74% heavier than control diploids, indicating that growth improvements from selective Breeding and triploidy were at least additive and could reduce the time to market by at least 10 months. Oyster type had no effect on the meat condition index, percent cavity volume, percent shell weight or cumulative mortality, but did affect whole weight/shell height ratios after 21 months grow-out.
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evaluation of the progeny of second generation sydney rock oyster saccostrea glomerata gould 1850 Breeding Lines for resistance to qx disease marteilia sydneyi
Aquaculture, 2003Co-Authors: John A. Nell, Rosalind E. HandAbstract:Abstract The progeny of second-generation Sydney rock oyster Saccostrea glomerata (Gould, 1850) Breeding Lines were tested for resistance to QX disease Marteilia sydneyi against a non-selected control. Mortality was reduced from 85.7±1.5% for the controls to 63.5±1.2% for the most improved Breeding Line. This is a reduction in mortality of 22% after only two generations of selection. These partially QX disease-resistant oysters in which M. sydneyi was found were also 21% heavier than controls. Selection for resistance to M. sydneyi is feasible and may be improved through further selection.
John A. Nell - One of the best experts on this subject based on the ideXlab platform.
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studies on triploid oysters in australia xiii performance of diploid and triploid sydney rock oyster saccostrea glomerata gould 1850 progeny from a third generation Breeding Line
Aquaculture, 2004Co-Authors: Rosalind E. Hand, John A. Nell, Peter A ThompsonAbstract:The performance of diploid and triploid selected and control oysters was compared at three sites to determine if the improvements in growth of selected oyster Line (L2) Sydney rock oysters were additive to the faster growth of triploids. After a grow-out period of 32 months, both mean whole weights and shell heights were in the order: L2 triploids>control triploids>L2 diploids>control diploids. Mean whole weights of different oyster Lines were all significantly different (p<0.05). A significant (p=0.02) site×Line interaction effect on oyster weights was also detected. On average, L2 triploids were 74% heavier than control diploids, indicating that growth improvements from selective Breeding and triploidy were at least additive and could reduce the time to market by at least 10 months. Oyster type had no effect on the meat condition index, percent cavity volume, percent shell weight or cumulative mortality, but did affect whole weight/shell height ratios after 21 months grow-out.
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evaluation of the progeny of second generation sydney rock oyster saccostrea glomerata gould 1850 Breeding Lines for resistance to qx disease marteilia sydneyi
Aquaculture, 2003Co-Authors: John A. Nell, Rosalind E. HandAbstract:Abstract The progeny of second-generation Sydney rock oyster Saccostrea glomerata (Gould, 1850) Breeding Lines were tested for resistance to QX disease Marteilia sydneyi against a non-selected control. Mortality was reduced from 85.7±1.5% for the controls to 63.5±1.2% for the most improved Breeding Line. This is a reduction in mortality of 22% after only two generations of selection. These partially QX disease-resistant oysters in which M. sydneyi was found were also 21% heavier than controls. Selection for resistance to M. sydneyi is feasible and may be improved through further selection.
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the sydney rock oyster saccostrea glomerata gould 1850 Breeding programme progress and goals
Aquaculture Research, 2000Co-Authors: John A. Nell, I R Smith, C C McpheeAbstract:In 1990, NSW Fisheries initiated a mass selection programme in Port Stephens, NSW, with the aim of Breeding faster growing Sydney rock oysters Saccostrea glomerata (Gould 1850). After two generations of selection, an average weight for age advantage of 18% (range 14-23% per Breeding Line) was achieved. This equates to a reduction of 3 months in the time taken to reach market size. Experiments are planned to determine how much of this 3 months advantage is additive to the 6 months advantage this laboratory has already obtained using triploid S. glomerata. A parallel set of S. glomerata Breeding Lines was established on the Georges River, NSW, to include selection for resistance to the protistan parasite Mikrocytos roughleyi, the causal agent of winter mortality. The programme was disrupted by the outbreak of QX disease Marteilia sydneyi, another protistan parasite, in 1994. In 1997, the Breeding programme was reorganized and expanded. New Lines were bred from oysters that had survived both QX and winter mortality. After one generation, a small improvement in resistance to QX has been recorded; however, the exposure of further generations to M. sydneyi will be required to confirm an increase in resistance.
Xiaotian Zhang - One of the best experts on this subject based on the ideXlab platform.
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characterization of a powdery mildew resistance gene in wheat Breeding Line 10v 2 and its application in marker assisted selection
Plant Disease, 2017Co-Authors: Liping Song, Shuoshuo Liang, Yuan Sheng, Guohao Han, Xiaotian ZhangAbstract:Powdery mildew, caused by Blumeria graminis f. sp. tritici, is a serious disease of wheat (Triticum aestivum L.) throughout the world. Host resistance is the most effective and preferred means for managing this disease. Line 10V-2, a wheat Breeding Line with superior agronomic performance, shows broad-spectrum seedling resistance to powdery mildew. Genetic analysis demonstrated that its resistance was controlled by a single dominant gene, tentatively designated Pm10V-2. This gene was localized near the documented Pm2 locus on chromosome 5DS using the simple sequence repeat (SSR) marker Cfd81. To saturate the marker map of Pm10V-2, more markers were developed using bulked segregant RNA-Seq. Two single-nucleotide polymorphism (SNP) markers (Swgi047 and Swgi064), three expressed sequence tag markers (Swgi007, Swgi029, and Swgi038), and one SSR marker (Swgi066) were polymorphic between the resistant and susceptible bulks and showed tightly linked to the Pm10V-2 gene. Pm10V-2 was flanked by the new developed markers Swgi064 and Swgi066 at genetic distances of 0.4 and 1.2 centimorgans (cM), respectively, and cosegregated with Swgi007 and Swgi038. The homologous sequence of Pm2a was cloned from 10V-2 based on a recent study. Although the sequence cloned from 10V-2 was completely identical to that of the reported Pm2a-related gene, they did not cosegregate but were separated at a genetic distance of 1.6 cM, indicating that Pm10V-2 was different from the reported of Pm2a-related gene. When inoculated with multiple B. graminis f. sp. tritici isolates, Pm10V-2 had a significantly different resistance spectrum from Pm2a and other powdery mildew (Pm) resistance genes at or near the Pm2 locus. Therefore, Pm10V-2 may be a new Pm2 allele or Pm2-linked gene. To use Pm10V-2 in marker-assisted selection (MAS) Breeding, seven markers applicable for MAS were confirmed, including three newly developed markers (Swgi029, Swgi038, and Swgi064) in the present work. Using these markers, a great number of resistant Lines with desirable agronomic performance were selected from crosses involving 10V-2, including the Breeding Line KM5016, which has been entered in the Regional trials in Hebei Province, China.
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molecular mapping of a new powdery mildew resistance gene pm2b in chinese Breeding Line km2939
Theoretical and Applied Genetics, 2015Co-Authors: Hongxing Xu, Xiuquan Li, Yunfeng Xu, Lihui Li, Yilin Zhou, Xiaotian Zhang, Diaoguo AnAbstract:Key message An allele of Pm2 for wheat powdery mildew resistance was identified in a putative Agropyron cristatum -derived Line and used in wheat Breeding programs.
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molecular mapping of a new powdery mildew resistance gene pm2b in chinese Breeding Line km2939
Theoretical and Applied Genetics, 2015Co-Authors: Yanmin Qie, Xiaotian Zhang, Qiaoling Luo, Yilin ZhouAbstract:An allele of Pm2 for wheat powdery mildew resistance was identified in a putative Agropyron cristatum -derived Line and used in wheat Breeding programs. Powdery mildew (caused by Blumeria graminis f. sp. tritici, Bgt) is one of the most devastating wheat diseases worldwide. It is important to exploit varied sources of resistance from common wheat and its relatives in resistance Breeding. KM2939, a Chinese Breeding Line, exhibits high resistance to powdery mildew at both the seedling and adult stages. It carries a single dominant powdery mildew resistance (Pm) allele of Pm2, designated Pm2b, the previous allelic designation Pm2 will be re-designated as Pm2a. Pm2b was mapped to chromosome arm 5DS and flanked by sequence characterized amplified region (SCAR) markers SCAR112 and SCAR203 with genetic distances of 0.5 and 1.3 cM, respectively. Sequence tagged site (STS) marker Mag6176 and simple sequence repeat (SSR) marker Cfd81 co-segregated with SCAR203. Pm2b differs in specificity from donors of Pm2a, Pm46 and PmLX66 on chromosome arm 5DS. Allelism tests indicated that Pm2b, Pm2a and PmLX66 are allelic. Therefore, Pm2b appears to be a new allele at the Pm2 locus. The closely linked markers were used to accelerate transfer of Pm2b to wheat cultivars in current production.
Yilin Zhou - One of the best experts on this subject based on the ideXlab platform.
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molecular mapping of a new powdery mildew resistance gene pm2b in chinese Breeding Line km2939
Theoretical and Applied Genetics, 2015Co-Authors: Hongxing Xu, Xiuquan Li, Yunfeng Xu, Lihui Li, Yilin Zhou, Xiaotian Zhang, Diaoguo AnAbstract:Key message An allele of Pm2 for wheat powdery mildew resistance was identified in a putative Agropyron cristatum -derived Line and used in wheat Breeding programs.
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molecular mapping of a new powdery mildew resistance gene pm2b in chinese Breeding Line km2939
Theoretical and Applied Genetics, 2015Co-Authors: Yanmin Qie, Xiaotian Zhang, Qiaoling Luo, Yilin ZhouAbstract:An allele of Pm2 for wheat powdery mildew resistance was identified in a putative Agropyron cristatum -derived Line and used in wheat Breeding programs. Powdery mildew (caused by Blumeria graminis f. sp. tritici, Bgt) is one of the most devastating wheat diseases worldwide. It is important to exploit varied sources of resistance from common wheat and its relatives in resistance Breeding. KM2939, a Chinese Breeding Line, exhibits high resistance to powdery mildew at both the seedling and adult stages. It carries a single dominant powdery mildew resistance (Pm) allele of Pm2, designated Pm2b, the previous allelic designation Pm2 will be re-designated as Pm2a. Pm2b was mapped to chromosome arm 5DS and flanked by sequence characterized amplified region (SCAR) markers SCAR112 and SCAR203 with genetic distances of 0.5 and 1.3 cM, respectively. Sequence tagged site (STS) marker Mag6176 and simple sequence repeat (SSR) marker Cfd81 co-segregated with SCAR203. Pm2b differs in specificity from donors of Pm2a, Pm46 and PmLX66 on chromosome arm 5DS. Allelism tests indicated that Pm2b, Pm2a and PmLX66 are allelic. Therefore, Pm2b appears to be a new allele at the Pm2 locus. The closely linked markers were used to accelerate transfer of Pm2b to wheat cultivars in current production.
Mary Ann Lila - One of the best experts on this subject based on the ideXlab platform.
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inhibition of pro inflammatory responses and antioxidant capacity of mexican blackberry rubus spp extracts
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Edith Oliva Cuevasrodriguez, Gad G Yousef, Pedro A Garciasaucedo, Jose Lopezmedina, Octavio Paredeslopez, Mary Ann Lila, Vermont P Dia, Elvira Gonzalez De MejiaAbstract:Total polyphenolic and anthocyanin- and proanthocyanidin-rich fractions from wild blackberry genotypes (WB-3, WB-7, WB-10, and WB-11), a domesticated noncommercial Breeding Line (UM-601), and a commercial cultivar (Tupy) were evaluated for inhibition of pro-inflammatory responses [nitric oxide (NO) production, inducible nitric oxide synthase (iNOS) expression, cyclooxygenase-2 (COX-2) expression, and prostaglandin E2 (PGE2)] in RAW 264.7 macrophages stimulated by lipopolysaccharide (LPS). At 50 μM [cyanidin-3-O-glucoside (C3G) or catechin equivalent], most fractions significantly (P < 0.05) inhibited all markers. The anthocyanin-rich fraction from WB-10 and the proanthocyanidin-rich fraction from UM-601 exhibited the highest NO inhibitory activities (IC50 = 16.1 and 15.1 μM, respectively). Proanthocyanidin-rich fractions from the wild WB-10 showed the highest inhibition of iNOS expression (IC50 = 8.3 μM). Polyphenolic-rich fractions from WB-7 and UM-601 were potent inhibitors of COX-2 expression (IC50 = 1...
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characterization of anthocyanins and proanthocyanidins in wild and domesticated mexican blackberries rubus spp
Journal of Agricultural and Food Chemistry, 2010Co-Authors: Edith Oliva Cuevasrodriguez, Gad G Yousef, Pedro A Garciasaucedo, Jose Lopezmedina, Octavio Paredeslopez, Mary Ann LilaAbstract:This study was designed to characterize and compare wild, commercial, and noncommercial cultivated blackberry genotypes grown in Michoacan, Mexico. Six genotypes, including WB-3, WB-7, WB-10, and WB-11 (all wild blackberry types), Tupy (a commercial cultivar), and UM-601 (a cultivated Breeding Line), were selected and profiled for anthocyanins and proanthocyanidins by separating extracts over Amberlite XAD-7 resin and Sephadex LH-20 columns. Subsequent high-performance liquid chromatography (HPLC) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analyses revealed that the major anthocyanin for all genotypes was cyanidin 3-O-glucoside. The proanthocyanidins (condensed tannins) were present in mono- to hexamer forms. Also, hydrolyzable tannins, ellagitannins, were characterized in the blackberry fruits. The average anthocyanin concentration in Sephadex LH-20 fractions was 49.2 mg/g in the commercial cultivar Tupy, while in the wild genotypes and the Breeding Line, the range was 361.3-494.9 mg/g (cyanidin 3-O-glucoside equivalent). The proanthocyanidin concentration varied widely among wild genotypes (417.5-1343.6 mg/g, catechin equivalent). This study demonstrated that the use of Amberlite XAD-7 followed by Sephadex LH-20 chromatography, with subsequent HPLC and LC-ESI-MS analyses, was able to effectively separate and characterize the diverse polyphenolics in blackberry genotypes. These results suggest that recommendations for dietary intake of blackberries for human health benefits need to take into account the source, because of the wide inherent variation in bioactive polyphenolic content in different blackberry genotypes.