The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
T. H. Kim - One of the best experts on this subject based on the ideXlab platform.
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Determination of human serum albumin using a single-walled carbon nanotube-FET modified with Bromocresol Green
Microchimica Acta, 2016Co-Authors: Bongsu Kim, T. H. KimAbstract:We developed a nanoscale-based assay for the detection of human serum albumin (HSA) by placing the HSA indicator probe, Bromocresol Green (BCG) on the single-walled carbon nanotubes on a field effect transistor (swCNT-FET) via simple coating. Bovine serum albumin (BSA) was used as a blocking agent to reduce non-specific binding. The selective binding of HSA onto BCG alters the charge of the dye and the surface, and this causes a change in the conductance of the swCNT-FET. The assay is highly sensitive and selective for HSA, with an 18.6 ng⋅mL−1 detection limit. The high sensitivity and selectivity of the assay is ascribed to appropriate BSA blocking and the instantaneous response of sensitive swCNT-FET to rapid BCG-HSA binding, which minimizes non-specific binding and overcomes the time-sensitivity of BCG-HSA binding, respectively.
Tsuyoshi Ueno - One of the best experts on this subject based on the ideXlab platform.
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the Bromocresol Green assay but not the modified Bromocresol purple assay overestimates the serum albumin concentration in nephrotic syndrome through reaction with α2 macroglobulin
Annals of Clinical Biochemistry, 2016Co-Authors: Tsuyoshi Ueno, Satoshi Hirayama, Masami Sugihara, Takashi MiidaAbstract:BackgroundThe Bromocresol Green (BCG) assay is commonly used for measuring albumin (ALB), but is affected by α1- and α2-globulins, which are elevated in systemic inflammation. The modified bromocre...
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albumin concentration determined by the modified Bromocresol purple method is superior to that by the Bromocresol Green method for assessing nutritional status in malnourished patients with inflammation
Annals of Clinical Biochemistry, 2013Co-Authors: Tsuyoshi Ueno, Satoshi Hirayama, Masayuki Ito, Emiko Nishioka, Yoshifumi Fukushima, Tomoaki Satoh, Mayumi Idei, Yuki Horiuchi, Hiromichi ShojiAbstract:BackgroundThe controlling nutritional status (CONUT) score (CS), a simple score for assessing nutritional status, is calculated using laboratory data, including serum albumin concentration. Although dye-binding assays such as the Bromocresol Green (BCG) and modified Bromocresol purple (mBCP) methods are widely used for albumin measurement, acute-phase proteins interfere with the BCG method.ObjectiveWe aimed to determine whether the choice of albumin assay affects assessment of nutritional status using CONUT scores (CSs).DesignWe measured serum albumin concentrations by the BCG (ALBBCG) and mBCP (ALBmBCP) methods in 44 malnourished inpatients, 27 of whom underwent nutritional intervention, and compared them to 30 age-matched healthy volunteers. In treated patients, CSs were calculated by ALBBCG (CS-BCG) and ALBmBCP (CS-mBCP).ResultsC-reactive protein (CRP) concentrations were positively correlated with the difference between ALBBCG and ALBmBCP in malnourished inpatients (r = 0.59, p < 0.001). CS-BCG was al...
Takashi Miida - One of the best experts on this subject based on the ideXlab platform.
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the Bromocresol Green assay but not the modified Bromocresol purple assay overestimates the serum albumin concentration in nephrotic syndrome through reaction with α2 macroglobulin
Annals of Clinical Biochemistry, 2016Co-Authors: Tsuyoshi Ueno, Satoshi Hirayama, Masami Sugihara, Takashi MiidaAbstract:BackgroundThe Bromocresol Green (BCG) assay is commonly used for measuring albumin (ALB), but is affected by α1- and α2-globulins, which are elevated in systemic inflammation. The modified bromocre...
Bongsu Kim - One of the best experts on this subject based on the ideXlab platform.
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Determination of human serum albumin using a single-walled carbon nanotube-FET modified with Bromocresol Green
Microchimica Acta, 2016Co-Authors: Bongsu Kim, T. H. KimAbstract:We developed a nanoscale-based assay for the detection of human serum albumin (HSA) by placing the HSA indicator probe, Bromocresol Green (BCG) on the single-walled carbon nanotubes on a field effect transistor (swCNT-FET) via simple coating. Bovine serum albumin (BSA) was used as a blocking agent to reduce non-specific binding. The selective binding of HSA onto BCG alters the charge of the dye and the surface, and this causes a change in the conductance of the swCNT-FET. The assay is highly sensitive and selective for HSA, with an 18.6 ng⋅mL−1 detection limit. The high sensitivity and selectivity of the assay is ascribed to appropriate BSA blocking and the instantaneous response of sensitive swCNT-FET to rapid BCG-HSA binding, which minimizes non-specific binding and overcomes the time-sensitivity of BCG-HSA binding, respectively.
Satoshi Hirayama - One of the best experts on this subject based on the ideXlab platform.
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the Bromocresol Green assay but not the modified Bromocresol purple assay overestimates the serum albumin concentration in nephrotic syndrome through reaction with α2 macroglobulin
Annals of Clinical Biochemistry, 2016Co-Authors: Tsuyoshi Ueno, Satoshi Hirayama, Masami Sugihara, Takashi MiidaAbstract:BackgroundThe Bromocresol Green (BCG) assay is commonly used for measuring albumin (ALB), but is affected by α1- and α2-globulins, which are elevated in systemic inflammation. The modified bromocre...
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albumin concentration determined by the modified Bromocresol purple method is superior to that by the Bromocresol Green method for assessing nutritional status in malnourished patients with inflammation
Annals of Clinical Biochemistry, 2013Co-Authors: Tsuyoshi Ueno, Satoshi Hirayama, Masayuki Ito, Emiko Nishioka, Yoshifumi Fukushima, Tomoaki Satoh, Mayumi Idei, Yuki Horiuchi, Hiromichi ShojiAbstract:BackgroundThe controlling nutritional status (CONUT) score (CS), a simple score for assessing nutritional status, is calculated using laboratory data, including serum albumin concentration. Although dye-binding assays such as the Bromocresol Green (BCG) and modified Bromocresol purple (mBCP) methods are widely used for albumin measurement, acute-phase proteins interfere with the BCG method.ObjectiveWe aimed to determine whether the choice of albumin assay affects assessment of nutritional status using CONUT scores (CSs).DesignWe measured serum albumin concentrations by the BCG (ALBBCG) and mBCP (ALBmBCP) methods in 44 malnourished inpatients, 27 of whom underwent nutritional intervention, and compared them to 30 age-matched healthy volunteers. In treated patients, CSs were calculated by ALBBCG (CS-BCG) and ALBmBCP (CS-mBCP).ResultsC-reactive protein (CRP) concentrations were positively correlated with the difference between ALBBCG and ALBmBCP in malnourished inpatients (r = 0.59, p < 0.001). CS-BCG was al...
