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Alaa S Amin - One of the best experts on this subject based on the ideXlab platform.
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Spectrophotometric Determination of Lornoxicam in Pure and in Pharmaceutical Formulations Using Ion-Associate Complex Formation
2015Co-Authors: Alaa S Amin, Gamal H. Ragab, Magda M. El-henawee, Amal F. SultanAbstract:Abstract: Simple, rapid, and sensitive spectrophotometric methods for the determination of lornoxicam (LX) in pure form and in pharmaceutical formulations has been developed. The methods are based on the formation of colored ion-associate complexes between LX and three different reagents, brilliant blue G (BBG), Bromocresol green (BCG), and Bromocresol Purple (BCP) using Britton-Robinson (B-R) buffer solutions. The colored complexes were measured exhibiting λmax at 537, 616 and 650 nm for BBG, BCP and BCG, respectively. The analytical parameters and their effects were investigated. The ion- associate complexes are intensely colored and very stable at room temperature. The calibration graphs were linear over the concentration range of 1-12 μg/mL for BBG and BCG and 1.0- 18 μg/mL for BCP. The stoichiometry of the ion-associate was found to be 1: 1 for all complexes. The proposed methods were successfully extended to pharmaceutical preparations in tablet dosage form. The results obtained by the proposed methods were comparable with those obtained by the official method
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Sensitive Spectrophotometric and Conductometric Methods for Determination of Candesartan Using Bromocresol Green and Bromocresol Purple by Ion-Pair Complex Formation
2015Co-Authors: Alaa S Amin, Hanna M. Salah, Gamal H. Ragab, Inass S. KamelAbstract:Two simple, sensitive, accurate, rapid spectrophotometric and conductometric methods were developed for the determination of candesartan (CAND) in pure and in its pharmaceutical preparation. The proposed methods depend upon the reaction of Bromocresol green (BCG) or Bromocresol Purple (BCP) with candesartan in phosphate buffered solution to form stable colored ion-pair complex, which was extracted in chloroform. The yellow colored complexes were determined at λmax 415, 405 nm with BCG, BCP, respectively. Using conductometric titration, candesartan could be evaluated in acetone. The optimizations of various experimental conditions were described. The results obtained showed good recoveries of 100.14 with relative standard deviations of 0.62. Applications of the proposed methods to representative pharmaceutical formulations are successfully presented compared with official methods.
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spectrophotometric determination of gemifloxacin mesylate moxifloxacin hydrochloride and enrofloxacin in pharmaceutical formulations using acid dyes
Journal of Automated Methods & Management in Chemistry, 2014Co-Authors: Ayman A Gouda, Alaa S Amin, Ragaa Elsheikh, Amira G YousefAbstract:Simple, rapid, and extractive spectrophotometric methods were developed for the determination of some fluoroquinolones antibiotics: gemifloxacin mesylate (GMF), moxifloxacin hydrochloride (MXF), and enrofloxacin (ENF) in pure forms and pharmaceutical formulations. These methods are based on the formation of ion-pair complexes between the basic drugs and acid dyes, namely, Bromocresol green (BCG), Bromocresol Purple (BCP), bromophenol blue (BPB), bromothymol blue (BTB), and methyl orange (MO) in acidic buffer solutions. The formed complexes were extracted with chloroform and measured at 420, 408, 416, 415, and 422 nm for BCG, BCP, BPB, BTB, and MO, respectively, for GMF; at 410, 415, 416, and 420 nm for BCP, BTB, BPB, and MO, respectively, for MXF; and at 419 and 414 nm for BCG and BTB, respectively, in case of ENF. The analytical parameters and their effects are investigated. Beer’s law was obeyed in the ranges 1.0–30, 1.0–20, and 2.0–24 μg mL−1 for GMF, MXF, and ENF, respectively. The proposed methods have been applied successfully for the analysis of the studied drugs in pure forms and pharmaceutical formulations. Statistical comparison of the results with the reference methods showed excellent agreement and indicated no significant difference in accuracy and precision.
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spectrophotometric determination of gatifloxacin in pure form and in pharmaceutical formulation
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2007Co-Authors: Alaa S Amin, Ayman A Gouda, Ragaa Elsheikh, Faten ZahranAbstract:Abstract Simple, rapid, and extractive spectrophotometric methods were developed for the determination of gatifloxacin (GT) in bulk and pharmaceutical dosage form. These methods are based on the formation of yellow ion-pair complexes between the basic nitrogen of the drug and three sulphonphthalein acid dyes, namely; Bromocresol green (BCG), Bromocresol Purple (BCP), bromophenol blue (BPB) and bromothymol blue (BTB) in phthalate buffer pH 3.0, 3.4 and 3.2, using BCG, BCP and (BPB or BTB), respectively. The formed complexes were extracted with chloroform and measured at 415, 417, 412 and 414 nm for BCG, BPB, BCP and BTB, respectively. The analytical parameters and their effects on the reported systems are investigated. The reactions were extremely rapid at room temperature and the absorbance values remains unchanged at 48 h for all reactions. Beer's law was obeyed in the ranges 2.0–20, 2.0–14 and 2.0–16 μg mL −1 for BCG, BCP and (BPB or BTB), respectively. The composition of the ion pairs was found 1:1 by Job's method. Beer's law validation, accuracy, precision, limits of detection, limits of quantification. The proposed methods have been applied successfully for the analysis of the drug bulk form and its dosage form. The results were in good agreement with those obtained by the official and reported methods.
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Quantitative Determination of Some Pharmaceutical Veterinary Formulations Using Bromocresol Purple and Bromocresol Green
Analytical Letters, 1997Co-Authors: Alaa S AminAbstract:Abstract A simple, sensitive, accurate and rapid spectrophotometric method for the determination of some anthelmintics that are used in veterinary medication is described, based on the formation of an ion pair complex with Bromocresol Purple (BCP) and Bromocresol green (BCG). Drugs analyzed are piperazine hexahydrate, tetramisole hydrochloride and metronidazole. The drugs were determined either in pure powdered forms or in pharmaceutical formulations using the standard addition technique. The sensitivity, precision and accuracy of the method was discussed and the results compared with the official method of British Pharmacopoeia (B.P. 1993).
Hiromichi Shoji - One of the best experts on this subject based on the ideXlab platform.
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albumin concentration determined by the modified Bromocresol Purple method is superior to that by the Bromocresol green method for assessing nutritional status in malnourished patients with inflammation
Annals of Clinical Biochemistry, 2013Co-Authors: Tsuyoshi Ueno, Satoshi Hirayama, Masayuki Ito, Emiko Nishioka, Yoshifumi Fukushima, Tomoaki Satoh, Mayumi Idei, Yuki Horiuchi, Hiromichi ShojiAbstract:BackgroundThe controlling nutritional status (CONUT) score (CS), a simple score for assessing nutritional status, is calculated using laboratory data, including serum albumin concentration. Although dye-binding assays such as the Bromocresol green (BCG) and modified Bromocresol Purple (mBCP) methods are widely used for albumin measurement, acute-phase proteins interfere with the BCG method.ObjectiveWe aimed to determine whether the choice of albumin assay affects assessment of nutritional status using CONUT scores (CSs).DesignWe measured serum albumin concentrations by the BCG (ALBBCG) and mBCP (ALBmBCP) methods in 44 malnourished inpatients, 27 of whom underwent nutritional intervention, and compared them to 30 age-matched healthy volunteers. In treated patients, CSs were calculated by ALBBCG (CS-BCG) and ALBmBCP (CS-mBCP).ResultsC-reactive protein (CRP) concentrations were positively correlated with the difference between ALBBCG and ALBmBCP in malnourished inpatients (r = 0.59, p < 0.001). CS-BCG was al...
Tomoaki Satoh - One of the best experts on this subject based on the ideXlab platform.
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albumin concentration determined by the modified Bromocresol Purple method is superior to that by the Bromocresol green method for assessing nutritional status in malnourished patients with inflammation
Annals of Clinical Biochemistry, 2013Co-Authors: Tsuyoshi Ueno, Satoshi Hirayama, Masayuki Ito, Emiko Nishioka, Yoshifumi Fukushima, Tomoaki Satoh, Mayumi Idei, Yuki Horiuchi, Hiromichi ShojiAbstract:BackgroundThe controlling nutritional status (CONUT) score (CS), a simple score for assessing nutritional status, is calculated using laboratory data, including serum albumin concentration. Although dye-binding assays such as the Bromocresol green (BCG) and modified Bromocresol Purple (mBCP) methods are widely used for albumin measurement, acute-phase proteins interfere with the BCG method.ObjectiveWe aimed to determine whether the choice of albumin assay affects assessment of nutritional status using CONUT scores (CSs).DesignWe measured serum albumin concentrations by the BCG (ALBBCG) and mBCP (ALBmBCP) methods in 44 malnourished inpatients, 27 of whom underwent nutritional intervention, and compared them to 30 age-matched healthy volunteers. In treated patients, CSs were calculated by ALBBCG (CS-BCG) and ALBmBCP (CS-mBCP).ResultsC-reactive protein (CRP) concentrations were positively correlated with the difference between ALBBCG and ALBmBCP in malnourished inpatients (r = 0.59, p < 0.001). CS-BCG was al...
Abdul Rashid Qureshi - One of the best experts on this subject based on the ideXlab platform.
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serum albumin as predictor of nutritional status in patients with esrd
Clinical Journal of The American Society of Nephrology, 2012Co-Authors: Thiane Gamaaxelsson, Olof Heimburger, Peter Stenvinkel, Peter Barany, Bengt Lindholm, Abdul Rashid QureshiAbstract:Summary Background and objectives Serum albumin is a widely used biomarker of nutritional status in patients with CKD; however, its usefulness is debated. This study investigated serum albumin and its correlation with several markers of nutritional status in incident and prevalent dialysis patients. Design, setting, participants, & measurements In a cross-sectional study, serum albumin (Bromocresol Purple), and other biochemical (serum creatinine), clinical (subjective global assessment [SGA]), anthropometric (handgrip strength; skinfold thicknesses), and densitometry (dual-energy x-ray absorptiometry) markers of nutritional status were assessed in 458 incident (61% male; mean age, 54±13 years; GFR, 6.6±0.3 ml/min per 1.73 m2; recruited 1994–2010) and 383 prevalent (56% male; mean age, 62±14 years; recruited 1989–2004) dialysis patients. Results In incident patients, serum albumin was correlated with age (β =−0.15; P 1; β=−0.19; P 1 (β=−0.16; P Conclusions In incident and prevalent dialysis patients, serum albumin correlates poorly with several markers of nutritional status. Thus, its value as a reliable marker of nutritional status in patients with ESRD is limited.
Marijn M. Speeckaert - One of the best experts on this subject based on the ideXlab platform.
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Binding of Bromocresol green and Bromocresol Purple to albumin in hemodialysis patients.
Clinical chemistry and laboratory medicine, 2017Co-Authors: Sigurd E. Delanghe, Wim Van Biesen, Nadeige Van De Velde, Sunny Eloot, Anneleen Pletinck, Eva Schepers, Griet Glorieux, Joris R. Delanghe, Marijn M. SpeeckaertAbstract:Background Colorimetric albumin assays based on binding to Bromocresol Purple (BCP) and Bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin. Methods Albumin was assayed using immunonephelometry on a BN II nephelometer and colorimetrically based on, respectively, BCP and BCG on a Modular P analyzer. Uremic toxins were assessed using high-performance liquid chromatography. Acute phase proteins (C-reactive protein and α1-acid glycoprotein) and plasma protein α2-macroglobulin were assayed nephelometrically. In parallel, Kt/V was calculated. Results Sixty-two serum specimens originating from hemodialysis patients were analyzed. Among the uremic toxins investigated, total para-cresyl sulfate (PCS) showed a significant positive correlation with the BCP/BCG ratio. The serum α1-acid glycoprotein concentration correlated negatively with the BCP/BCG ratio. The BCP/BCG ratio showed also a negative correlation with Kt/V. Conclusions In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role.
