The Experts below are selected from a list of 8196 Experts worldwide ranked by ideXlab platform
Qu Hai-lon - One of the best experts on this subject based on the ideXlab platform.
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The construction of Brucella Melitensis 16M Omp25 gene in eukaryotic expression vector
Chinese journal of veterinary science, 2013Co-Authors: Qu Hai-lonAbstract:In this research,Brucella Melitensis 16M Omp25 gene was amplified by PCR.The purified PCR products were ligated with pMD18-T simple vector.After enzyme digestion and sequence analysis,the correct pMD-18T-Omp25 gene was digested by EcoRⅠand Xho,and cloned into the eukaryotic expression vector pCMV-HA which was digested by the same enzymes.The result shows that the eukaryotic expression vector of Brucella Melitensis 16M Omp25 gene was constructed successfully,which laid the foundation for the Omp25 function analysis.
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Construction of a bait plasmid containing Brucella Melitensis 16M VjbR gene in a yeast two-hybrid system and evaluation of its toxicity and self-activation
Chinese journal of veterinary science, 2013Co-Authors: Qu Hai-lonAbstract:To construct a yeast expression vector of Brucella Melitensis 16M VjbR gene using the Sos-recruitment system(SRS),the coding sequence of Brucella Melitensis 16M VjbR gene was amplified by PCR and cloned into the yeast expression plasmid pSos.The recombinant bait plasmid pSos-VjbR was verified by sequencing before transformation into competent yeast cells.The effects of the expression product on the yeast cell growth and activation of the reporter gene were evaluated.The results show the yeast expression vector of Brucella Melitensis 16M VjbR gene was constructed sucessfully.The recombinant bait plasmid showed no toxic effect on yeast cdc25H cells without a self-activation of the reporter gene.The SRS can be used to study the proteins interacting with Brucella Melitensis 16M VjbR protein and provides a means for obtaining insight into the pathogenic mechanism of Brucella Melitensis 16M.
Wang Xing-long - One of the best experts on this subject based on the ideXlab platform.
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Construction of a bait plasmid containing Brucella Melitensis 16M VjbR gene in a yeast two-hybrid system and evaluation of its toxicity and self-activation
Chinese journal of veterinary science, 2012Co-Authors: Wang Xing-longAbstract:To construct a yeast expression vector of Brucella Melitensis 16M VjbR gene using the Sos-recruitment system(SRS),the coding sequence of Brucella Melitensis 16M VjbR was amplified by PCR and cloned into the yeast expression plasmid pSos.The recombinant bait plasmid pSos-VjbR was verified by sequencing before transformation into competent yeast cells.The effects of the expression product on the yeast cell growth and activation of the reporter gene were evaluated.The results show the yeast expression vector of Brucella Melitensis 16M VjbR gene was constructed sucessfully.The recombinant bait plasmid showed no toxic effect on yeast cdc25H cells without a self-activation of the reporter gene.The SRS can be used to study the proteins interacting with Brucella Melitensis 16M VjbR protein and provides a means for obtaining insight into the pathogenic mechanism of Brucella Melitensis 16M.
Y. Izci - One of the best experts on this subject based on the ideXlab platform.
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Lumbosacral spinal epidural abscess caused by Brucella Melitensis.
Acta neurochirurgica, 2005Co-Authors: Y. IzciAbstract:Spinal epidural abscess is a rare condition that can lead to irreversible complications and death if untreated. Rarely, Brucella Melitensis may produce spondylodiscitis, which may be complicated by spinal epidural abscess. We report a case of lumbosacral spinal epidural abscess caused by Brucella Melitensis. Spinal brucellosis should be kept in mind in the differential diagnosis of low back pain.
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Case Report Lumbosacral spinal epidural abscess caused by Brucella Melitensis
2005Co-Authors: Y. IzciAbstract:Summary Spinal epidural abscess is a rare condition that can lead to irreversible complications and death if untreated. Rarely, Brucella Melitensis may produce spondylodiscitis, which may be complicated by spinal epidural abscess. We report a case of lumbosacral spinal epidural abscess caused by Brucella Melitensis. Spinal brucellosis should be kept in mind in the differential diagnosis of low back pain.
Guler Yayli - One of the best experts on this subject based on the ideXlab platform.
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Investigation of the in vitro activities of various antibiotics against Brucella Melitensis strains
Turkish Journal of Medical Sciences, 2012Co-Authors: Onur Kaya, Fusun Zeynep Akcam, Guler YayliAbstract:To assess the antimicrobial susceptibility of Brucella Melitensis strains in our region. Brucellosis is a common disease in Turkey. Moreover, difficulties are encountered in the treatment process, such as long-lasting therapy, relapses, and side effects of drugs. Hence, novel treatment approaches and the susceptibility of current antibiotics are vital. Materials and methods: Thirty-four Brucella spp. isolated from blood samples of patients with brucellosis were included. Minimal inhibitory concentrations for tetracycline, rifampicin, streptomycin, ciprofloxacin, doxycycline, ceftriaxone, and levofloxacin were detected by broth microdilution. Results: Tetracycline had the lowest and streptomycin the highest MIC50 and MIC90 values, respectively. A total of 5 strains were intermediate-susceptible and 1 strain was resistant to rifampicin. Conclusion: Doxycycline and tetracycline seem to be quite effective antibiotics against Brucella Melitensis strains. Although streptomycin and rifampicin have high MIC levels against Brucella Melitensis strains in our region, inconsistencies between in vitro susceptibility and in vivo activity should be considered.
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A rare agent of pneumonia: Brucella Melitensis
Tuberkuloz ve toraks, 2008Co-Authors: Önder Öztürk, Zeynep Akçam, Unal Sahin, Ahmet Bircan, Ahmet Akkaya, Guler YayliAbstract:Brucellosis remains a world-wide public health problem especially in developing brucellosis remains a world-wide public health problem especially in developing countries. Although involvement of the respiratory system in brucellosis is an acknowledged but rare event, its clinical manifestations and focal complications are often troublesome in making a diagnosis. Herein, we report a pneumonia case which proved due to Brucella Melitensis in a 57-year-old man who presented with chronic cough, sputum, hemoptysis and fever.
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Medically treated splenic abscess due to Brucella Melitensis.
Scandinavian journal of infectious diseases, 2002Co-Authors: Guler Yayli, Mehmet Isler, Orhan OyarAbstract:Brucellosis may lead to complications that affect different organ systems, including the liver and spleen. In acute disease, hepatosplenic abscess is a rare complication. We report herein a woman with splenic abscess due to acute Brucella Melitensis infection who was successfully treated with antibiotics alone.
Pablo Yagupsky - One of the best experts on this subject based on the ideXlab platform.
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Bartholin's Gland Abscess Caused by Brucella Melitensis
Journal of clinical microbiology, 2004Co-Authors: Neha Peled, Yohai David, Pablo YagupskyAbstract:We report herein a case of Bartholin's gland abscess caused by Brucella Melitensis. Clinical microbiology laboratory workers in areas where this disease is endemic should be familiar with the bacteriological features of this organism and consider the possibility of a Brucellar etiology in a broad range of clinical settings.
