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J P Dubey - One of the best experts on this subject based on the ideXlab platform.
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shedding of neospora caninum oocysts by dogs fed tissues from naturally infected water Buffaloes bubalus bubalis from brazil
Veterinary Parasitology, 2004Co-Authors: Aline Aparecida Rezende Rodrigues, Daniel Moura De Aguiar, C Sreekumar, M C B Vianna, Solange Maria Gennari, D E Hill, Katarzyna B Miska, J P DubeyAbstract:Attempts were made to isolate Neospora caninum from naturally infected water Buffaloes (Bubalus bubalis) from Brazil. Brains from six Buffaloes with indirect fluorescent antibodies (>1:100) to N. caninum were used to isolate the parasite by bioassay in dogs and gerbils followed by in vitro culture. Shedding of Neospora-like oocysts was noticed in dogs fed brains from three Buffaloes (isolate designation NcBrBuf-1, 2 and 4). Two more isolates (NcBrBuf-3 and 5) were obtained by in vitro culture of the brains of gerbils previously infected with brains of two other Buffaloes. The identity of the isolates was confirmed by biological and molecular methods. The isolates were found to be non-pathogenic to gerbils. All five isolates amplified the gene 5 amplicons using Neospora-specific PCR assay. The sequences of gene 5 fragments and the common toxoplasmatiid ITS-1 fragments were analyzed. The dynamics of oocyst production in the dogs indicate that water Buffaloes are natural intermediate hosts for N. caninum. This is the first report of isolation of N. caninum from water Buffaloes.
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seroprevalence of neospora caninum in female water Buffaloes bubalus bubalis from the southeastern region of brazil
Veterinary Parasitology, 2001Co-Authors: Takako Ueki Fujii, J P Dubey, Nobuko Kasai, Sandra Mayumi Nishi, Solange Maria GennariAbstract:Abstract Antibodies to Neospora caninum were assayed in sera of 222 female water Buffaloes from Ribeira Valley of Sao Paulo State, Brazil, using an indirect fluorescent antibody test (IFAT) and Neospora agglutination test (NAT). IFAT antibodies were found in 64% of Buffaloes with titers of 1:25 (42 Buffaloes), 1:50 (53 Buffaloes), 1:100 (31 Buffaloes), 1:200 (10 Buffaloes), 1:400 (3 Buffaloes), or ≥1:800 (3 Buffaloes). NAT antibodies were found in 53% of Buffaloes; in titers of 1:40 in 52 Buffaloes, 1:80 in 27 Buffaloes, 1:160 in 21 Buffaloes, and ≥1:320 in 17 Buffaloes. Results indicate a high prevalence of N. caninum exposure in water Buffaloes in Brazil and warrant an investigation of the role of N. caninum as an abortifacient in water Buffaloes.
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seroprevalence of antibodies to neospora caniuum and toxoplasma gondii in water Buffaloes bubalus bubalis from egypt
International Journal for Parasitology, 1998Co-Authors: J P Dubey, S Romand, M Hilali, O C H Kwok, P ThulliezAbstract:Abstract Sera from 75 water Buffaloes from Egypt were examined using a direct agglutination test incorporating mercaptoethanol for antibodies to Neospora caninum and Toxoplasma gondii . Antibodies to N. caninum were found in 51 (68%) of 75 Buffaloes in titres of 1:20 (six Buffaloes), 1:40 (15 Buffaloes), 1:160 (one buffalo), 1:320 (one buffalo) and ≥1:640 (28 Buffaloes), using N. caninum formalin-preserved whole tachyzoites as antigen. Antibodies to T. gondii were not found in a 1:100 dilution of serum of any of the 75 Buffaloes, using T. gondii as antigen, indicating specificity in the detection of antibodies to N. caninum . This is the first report of N. caninum prevalence in water Buffaloes, which are economically very important domestic animals in developing countries.
P Thulliez - One of the best experts on this subject based on the ideXlab platform.
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seroprevalence of antibodies to neospora caniuum and toxoplasma gondii in water Buffaloes bubalus bubalis from egypt
International Journal for Parasitology, 1998Co-Authors: J P Dubey, S Romand, M Hilali, O C H Kwok, P ThulliezAbstract:Abstract Sera from 75 water Buffaloes from Egypt were examined using a direct agglutination test incorporating mercaptoethanol for antibodies to Neospora caninum and Toxoplasma gondii . Antibodies to N. caninum were found in 51 (68%) of 75 Buffaloes in titres of 1:20 (six Buffaloes), 1:40 (15 Buffaloes), 1:160 (one buffalo), 1:320 (one buffalo) and ≥1:640 (28 Buffaloes), using N. caninum formalin-preserved whole tachyzoites as antigen. Antibodies to T. gondii were not found in a 1:100 dilution of serum of any of the 75 Buffaloes, using T. gondii as antigen, indicating specificity in the detection of antibodies to N. caninum . This is the first report of N. caninum prevalence in water Buffaloes, which are economically very important domestic animals in developing countries.
B Brenig - One of the best experts on this subject based on the ideXlab platform.
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analysis of mitochondrial d loop region casts new light on domestic water buffalo bubalus bubalis phylogeny
Molecular Phylogenetics and Evolution, 2004Co-Authors: Gerold Kierstein, Marcelo Vallinoto, Artur Silva, Maria Paula Cruz Schneider, Leopoldo Iannuzzi, B BrenigAbstract:The phylogeny of water Buffaloes (Bubalus bubalis) is still a matter of discussion, especially if the two types of domestic water buffalo (swamp and river) derived from different domestication events or if they are products of human selection. To obtain more insight, we analyzed the entire mitochondrial D-loop region of 80 water Buffaloes of four different breeds, i.e., 19 swamp Buffaloes (Carabao) and 61 river Buffaloes (Murrah, Jafarabadi, and Mediterranean), sampled in Brazil and Italy. We detected 36 mitochondrial haplotypes with 128 polymorphic sites. Pooled with published data of South-East Asian and Australian water Buffaloes and based on comprehensive median-joining network and population demography analyses we show evidence that both river and swamp Buffaloes decent from one domestication event, probably in the Indian subcontinent. However, the today swamp Buffaloes have an unravelled mitochondrial history, which can be explained by introgression of wild water buffalo mtDNA into domestic stocks. We are also discussing indications for an independent domestication of Buffaloes in China.
Solange Maria Gennari - One of the best experts on this subject based on the ideXlab platform.
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shedding of neospora caninum oocysts by dogs fed tissues from naturally infected water Buffaloes bubalus bubalis from brazil
Veterinary Parasitology, 2004Co-Authors: Aline Aparecida Rezende Rodrigues, Daniel Moura De Aguiar, C Sreekumar, M C B Vianna, Solange Maria Gennari, D E Hill, Katarzyna B Miska, J P DubeyAbstract:Attempts were made to isolate Neospora caninum from naturally infected water Buffaloes (Bubalus bubalis) from Brazil. Brains from six Buffaloes with indirect fluorescent antibodies (>1:100) to N. caninum were used to isolate the parasite by bioassay in dogs and gerbils followed by in vitro culture. Shedding of Neospora-like oocysts was noticed in dogs fed brains from three Buffaloes (isolate designation NcBrBuf-1, 2 and 4). Two more isolates (NcBrBuf-3 and 5) were obtained by in vitro culture of the brains of gerbils previously infected with brains of two other Buffaloes. The identity of the isolates was confirmed by biological and molecular methods. The isolates were found to be non-pathogenic to gerbils. All five isolates amplified the gene 5 amplicons using Neospora-specific PCR assay. The sequences of gene 5 fragments and the common toxoplasmatiid ITS-1 fragments were analyzed. The dynamics of oocyst production in the dogs indicate that water Buffaloes are natural intermediate hosts for N. caninum. This is the first report of isolation of N. caninum from water Buffaloes.
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seroprevalence of neospora caninum in female water Buffaloes bubalus bubalis from the southeastern region of brazil
Veterinary Parasitology, 2001Co-Authors: Takako Ueki Fujii, J P Dubey, Nobuko Kasai, Sandra Mayumi Nishi, Solange Maria GennariAbstract:Abstract Antibodies to Neospora caninum were assayed in sera of 222 female water Buffaloes from Ribeira Valley of Sao Paulo State, Brazil, using an indirect fluorescent antibody test (IFAT) and Neospora agglutination test (NAT). IFAT antibodies were found in 64% of Buffaloes with titers of 1:25 (42 Buffaloes), 1:50 (53 Buffaloes), 1:100 (31 Buffaloes), 1:200 (10 Buffaloes), 1:400 (3 Buffaloes), or ≥1:800 (3 Buffaloes). NAT antibodies were found in 53% of Buffaloes; in titers of 1:40 in 52 Buffaloes, 1:80 in 27 Buffaloes, 1:160 in 21 Buffaloes, and ≥1:320 in 17 Buffaloes. Results indicate a high prevalence of N. caninum exposure in water Buffaloes in Brazil and warrant an investigation of the role of N. caninum as an abortifacient in water Buffaloes.
Toshihiko Nakao - One of the best experts on this subject based on the ideXlab platform.
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some characteristics of vaginal prolapse in nepali Buffaloes
Journal of Veterinary Medical Science, 2003Co-Authors: Shyam Kishore Sah, Toshihiko NakaoAbstract:Prolapse of vagina is one of the important maternal abnormalities during pregnancy in cattle and Buffaloes. A field investigation was carried out on 26 Murrah graded Buffaloes to study clinical characteristics of vaginal prolapse in Buffaloes in Nepal. Fifty-seven percent of the 26 Buffaloes with vaginal prolapse were either heifers or in first lactation. Sixty-five percent of the cases were in seventh month of pregnancy or later. About three quarters of the cases occurred between June and October. Twelve cases (63%) of the 19 animals excluding 7 heifers had a history of vaginal prolapse in previous gestations. A half of the Buffaloes were showing prolapse of the vagina even when they were in standing position and showing moderate or vigorous straining. After the conventional treatments, twenty-three Buffaloes retained the replaced vagina and calved normally. One animal aborted although the vagina was retained. Two Buffaloes had severest degree of vaginal prolapse complicated with edema, injury and cyanosis, and they did not respond to the treatment. The two Buffaloes had frequently recurrent prolapse and subsequently died. Early detection and prompt treatment may be imperative to control the vaginal prolapse in Buffaloes.
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Characteristics of Vaginal Prolapse in Buffaloes
Journal of the Institute of Agriculture and Animal Science, 2002Co-Authors: Sk Sah, Toshihiko NakaoAbstract:Prolapse of vagina is one of the important maternal abnormalities during pregnancy in cattle and Buffaloes. A field investigation was carried out to show some clinical characteristics of vaginal prolapse in Buffaloes in Nepal as related to parity, stage of pregnancy, seasonal variation, degree of the prolapse, complications, treatment and prognosis. Fifty-seven percent of 26 Buffaloes with vaginal prolapse were heifers and cows in the first lactation. Fifty-seven percent of the cases were in seven month of pregnancy or later. About three quarters of the cases were diagnosed during a period between June and October including rainy season. Twelve cases (63%) of the nineteen had a history of vaginal prolapse in previous gestation periods. A half of the Buffaloes were showing prolapse of the vagina even when they were in standing position and showing moderate or vigorous staining. After the conventional treatments with calcium and phosphorus, twenty-three Buffaloes retained the replaced vagina and calved normally. One of the aborted while the vagina was retained, while the other two Buffaloes with the severest degree of the prolapse showing severe edema with injury and cyanosis, the prolapse recurred due to strong continuous straining and died subsequently. Early detection and prompt treatment at a less progressive degree of the prolapse may be imperative to control the vaginal prolapse in Buffaloes. Keywords: Buffaloes, pregnancy, vaginal prolapse J. Inst. Agric. Anim. Sci. 23:71-75
