The Experts below are selected from a list of 1722 Experts worldwide ranked by ideXlab platform
David Sassoon - One of the best experts on this subject based on the ideXlab platform.
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expression analysis of the Stem Cell marker pw1 peg3 reveals a cd34 negative progenitor population in the hair follicle
Stem Cells, 2017Co-Authors: Vanessa Besson, Giovanna Marazzi, Sergiy Kyryachenko, Peggy Janich, Salvador Aznar Benitah, David SassoonAbstract:Pw1/Peg3 is a parentally imprinted gene expressed in adult Stem Cells in every tissue thus far examined including the Stem Cells of the hair follicle. Using a Pw1/Peg3 reporter mouse, we carried out a detailed dissection of the Stem Cells in the Bulge, which is a major Stem Cell compartment of the hair follicle in mammalian skin. We observed that PW1/Peg3 expression initiates upon placode formation during fetal development, coincident with the establishment of the Bulge Stem Cells. In the adult, we observed that PW1/Peg3 expression is found in both CD34+ and CD34- populations of Bulge Stem Cells. We demonstrate that both populations can give rise to new hair follicles, reconstitute their niche, and self-renew. These results demonstrate that PW1/Peg3 is a reliable marker of the full population of follicle Stem Cells and reveal a novel CD34- Bulge Stem-Cell population. Stem Cells 2017;35:1015-1027.
Enkui Duan - One of the best experts on this subject based on the ideXlab platform.
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expansion of hair follicle Stem Cells sticking to isolated sebaceous glands to generate in vivo epidermal structures
Cell Transplantation, 2016Co-Authors: Huishan Zhang, Shoubing Zhang, Shuang Liu, Xiaohua Lei, Lina Ning, Yujing Cao, Huashan Zhao, Jingqiao Qiao, Enkui DuanAbstract:Hair follicle Stem Cells (HFSCs) are considered one of the useful donor Cell types for skin regenerative medicine owing to their robust proliferative capacity and multipotency. However, methods for easily and effectively obtaining HFSCs from a limited skin biopsy are still lacking. Here we report a novel approach for obtaining a subpopulation of HFSCs from a small skin sample from the rat tail, which uses the sebaceous glands (SGs) to capture the adjacent HFSCs. By means of organ culture, keratinocytes were expanded from the detached SGs, which also included adherent HFSCs from the hair follicle that could be passaged at the single-Cell level. These SG-captured keratinocytes strongly expressed the basal layer markers K14, integrin α6, and p63; the Bulge Stem Cell marker K15; and the upper isthmus Stem Cell marker Plet1. Furthermore, we reconstituted new epidermis, hair follicles, and SGs from the SG-captured keratinocytes using an easily operated, modified skin reconstitution assay based on silicone gel sheeting. This study suggests that the SGs could be an accessible capturer to harvest the adjacent HFSC subpopulation, particularly when the donor tissue is limited.
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hair follicle Stem Cells derived from single rat vibrissa via organ culture reconstitute hair follicles in vivo
Cell Transplantation, 2012Co-Authors: Shoubing Zhang, Huishan Zhang, Shuang Liu, Shu Liu, Ying Zhang, Xiaohua Lei, Lina Ning, Yujing Cao, Enkui DuanAbstract:Hair follicle Stem Cells (HFSCs) are potentially useful for the treatment of skin injuries and diseases. To achieve clinical application, a prerequisite must be accomplished: harvesting enough HFSCs from limited skin biopsy. The commonly used sorting approach for isolating HFSCs, however, suffers from its intrinsic disadvantages, such as requirement of large-scale skin biopsy. Here, we report an efficient organ culture method to isolate and expand rat HFSCs from limited skin biopsy and these HFSCs could reconstitute the epidermis and the hair follicles (HFs). Seventy-three percent of cultured HFs formed hair follicle Stem Cell colonies from the Bulge, and a single hair follicle provided all the HFSCs used in this research, demonstrating the high efficiency of this method. Quantitative RT-PCR and immunofluorescent staining results revealed that these Stem Cells obtained from the Bulge highly expressed basal layer markers K14 and alpha-6 integrin, epithelial Stem Cell marker P63, and Bulge Stem Cell marker ...
Vanessa Besson - One of the best experts on this subject based on the ideXlab platform.
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expression analysis of the Stem Cell marker pw1 peg3 reveals a cd34 negative progenitor population in the hair follicle
Stem Cells, 2017Co-Authors: Vanessa Besson, Giovanna Marazzi, Sergiy Kyryachenko, Peggy Janich, Salvador Aznar Benitah, David SassoonAbstract:Pw1/Peg3 is a parentally imprinted gene expressed in adult Stem Cells in every tissue thus far examined including the Stem Cells of the hair follicle. Using a Pw1/Peg3 reporter mouse, we carried out a detailed dissection of the Stem Cells in the Bulge, which is a major Stem Cell compartment of the hair follicle in mammalian skin. We observed that PW1/Peg3 expression initiates upon placode formation during fetal development, coincident with the establishment of the Bulge Stem Cells. In the adult, we observed that PW1/Peg3 expression is found in both CD34+ and CD34- populations of Bulge Stem Cells. We demonstrate that both populations can give rise to new hair follicles, reconstitute their niche, and self-renew. These results demonstrate that PW1/Peg3 is a reliable marker of the full population of follicle Stem Cells and reveal a novel CD34- Bulge Stem-Cell population. Stem Cells 2017;35:1015-1027.
Ana Aranda - One of the best experts on this subject based on the ideXlab platform.
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thyroid hormone signaling controls hair follicle Stem Cell function
Molecular Biology of the Cell, 2015Co-Authors: Constanza Contrerasjurado, Corina Lorz, Laura Garciaserrano, Jesus M Paramio, Ana ArandaAbstract:Observations in thyroid patients and experimental animals show that the skin is an important target for the thyroid hormones. We previously showed that deletion in mice of the thyroid hormone nuclear receptors TRα1 and TRβ (the main thyroid hormone-binding isoforms) results in impaired epidermal proliferation, hair growth, and wound healing. Stem Cells located at the Bulges of the hair follicles are responsible for hair cycling and contribute to the regeneration of the new epidermis after wounding. Therefore a reduction in the number or function of the Bulge Stem Cells could be responsible for this phenotype. Bulge Cells show increased levels of epigenetic repressive marks, can retain bromodeoxyuridine labeling for a long time, and have colony-forming efficiency (CFE) in vitro. Here we demonstrate that mice lacking TRs do not have a decrease of the Bulge Stem Cell population. Instead, they show an increase of label-retaining Cells (LRCs) in the Bulges and enhanced CFE in vitro. Reduced activation of Stem Cells leading to their accumulation in the Bulges is indicated by a strongly reduced response to mobilization by 12-O-tetradecanolyphorbol-13-acetate. Altered function of the Bulge Stem Cells is associated with aberrant activation of Smad signaling, leading to reduced nuclear accumulation of β-catenin, which is crucial for Stem Cell proliferation and mobilization. LRCs of TR-deficient mice also show increased levels of epigenetic repressive marks. We conclude that thyroid hormone signaling is an important determinant of the mobilization of Stem Cells out of their niche in the hair Bulge. These findings correlate with skin defects observed in mice and alterations found in human thyroid disorders.
Fiona M Watt - One of the best experts on this subject based on the ideXlab platform.
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β catenin and hedgehog signal strength can specify number and location of hair follicles in adult epidermis without recruitment of Bulge Stem Cells
Developmental Cell, 2005Co-Authors: Violeta Silvavargas, Kristin M Braun, Cristina Lo Celso, Adam Giangreco, Tyler Ofstad, David M Prowse, Fiona M WattAbstract:Summary Using K14ΔNβ-cateninER transgenic mice, we show that short-term, low-level β-catenin activation stimulates de novo hair follicle formation from sebaceous glands and interfollicular epidermis, while only sustained, high-level activation induces new follicles from preexisting follicles. The Hedgehog pathway is upregulated by β-catenin activation, and inhibition of Hedgehog signaling converts the low β-catenin phenotype to wild-type epidermis and the high phenotype to low. β-catenin-induced follicles contain clonogenic keratinocytes that express Bulge markers; the follicles induce dermal papillae and provide a niche for melanocytes, and they undergo 4OHT-dependent cycles of growth and regression. New follicles induced in interfollicular epidermis are derived from that Cellular compartment and not through Bulge Stem Cell migration or division. These results demonstrate the remarkable capacity of adult epidermis to be reprogrammed by titrating β-catenin and Hedgehog signal strength and establish that Cells from interfollicular epidermis can acquire certain characteristics of Bulge Stem Cells.
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β-Catenin and Hedgehog Signal Strength Can Specify Number and Location of Hair Follicles in Adult Epidermis without Recruitment of Bulge Stem Cells
Developmental cell, 2005Co-Authors: Violeta Silva-vargas, Kristin M Braun, Cristina Lo Celso, Adam Giangreco, Tyler Ofstad, David M Prowse, Fiona M WattAbstract:Using K14deltaNbeta-cateninER transgenic mice, we show that short-term, low-level beta-catenin activation stimulates de novo hair follicle formation from sebaceous glands and interfollicular epidermis, while only sustained, high-level activation induces new follicles from preexisting follicles. The Hedgehog pathway is upregulated by beta-catenin activation, and inhibition of Hedgehog signaling converts the low beta-catenin phenotype to wild-type epidermis and the high phenotype to low. beta-catenin-induced follicles contain clonogenic keratinocytes that express Bulge markers; the follicles induce dermal papillae and provide a niche for melanocytes, and they undergo 4OHT-dependent cycles of growth and regression. New follicles induced in interfollicular epidermis are derived from that Cellular compartment and not through Bulge Stem Cell migration or division. These results demonstrate the remarkable capacity of adult epidermis to be reprogrammed by titrating beta-catenin and Hedgehog signal strength and establish that Cells from interfollicular epidermis can acquire certain characteristics of Bulge Stem Cells.
