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B S Gill - One of the best experts on this subject based on the ideXlab platform.
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Chromosome identifiCation in plantago ovata forsk through C Banding and fish
Current Science, 2002Co-Authors: Manoj K Dhar, Sanjana Kaul, Bernd Friebe, B S GillAbstract:Plantago ovata, the sourCe of Psyllium husk has a haploid Complement of just four Chromosomes. SomatiC Chromosomes of this speCies were studied to delineate C-Banding pattern and physiCal mapping of repetitive, telomeriC and rDNA sites to provide landmarks for Chromosome identifiCation. Whereas C-Banding faCilitated identifiCation of all the Chromosomes, the fluoresCenCe in situ hybridization (FISH) with 5S rRNA gene probe helps in the identifiCation of Chromosome 1, and 45S rRNA probe identifies Chromosomes 3 and 4. Chromosome 2 is unique in the sense that it does not bear any rDNA FISH sites. The major portion of the genome is Comprised of repetitive DNA sequenCes.
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C Banding variation in the moroCCan oat speCies avena agadiriana 2n 4x 28
Theoretical and Applied Genetics, 1996Co-Authors: Eric N Jellen, B S GillAbstract:The C-Banding teChnique was used to desCribe the Chromosomes of a relatively reCently-disCovered MoroCCan oat speCies, Avena agadiriana (2n=4x=28). A substantial amount of polymorphism for arm ratios and C-Banding patterns was observed among five aCCessions of this speCies. However a Common set of ten putatively homologous Chromosomes was identifiable among the five aCCessions. The Chromosomes of A. Agadiriana do not Closely matCh those of any of the previously desCribed diploid or tetraploid oat speCies in terms of their arm ratios and C-Banding patterns. However, their overall C-banded appearanCe generally resembles the A/B/D groups of Chromosomes of Avena speCies, rather than the more hetroChromatiC C genomes. ImpliCations of these findings in terms of Chromosome evolution in the genus Avena are disCussed.
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CharaCterization of hordeum Chilense Chromosomes by C Banding and in situ hybridization using highly repeated dna probes
Genome, 1995Co-Authors: A Cabrera, B Friebe, Jiming Jiang, B S GillAbstract:C-Banding patterns of Hordeum Chilense and of TritiCum aestivum 'Chinese Spring' – H. Chilense disomiC addition lines were analyzed and Compared with in situ hybridization patterns using a biotin-labeled highly repetitive TritiCum tausChii DNA sequenCe, pAs1, and a wheat 18S–26S rDNA probe. All seven H. Chilense Chromosomes pairs and the added H. Chilense Chromosomes present in the addition lines were identified by their CharaCteristiC C-Banding pattern. Chromosome morphology and Banding patterns were similar to those of the Corresponding Chromosomes present in the parent H. Chilense aCCession. A C-banded karyotype of the added H. Chilense Chromosomes was ConstruCted and Chromosome lengths, arm ratios, and relative length, as Compared with Chromosome 3B, were determined. The probe pAs1 was found to hybridize to speCifiC areas on telomeres and interstitial sites along the Chromosomes, allowing the identifiCation of all seven pairs of the H. Chilense Chromosomes. Comparison of the patterns of distribution o...
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Comparison of C Banding patterns and in situ hybridization sites using highly repetitive and total genomiC rye dna probes of imperial rye Chromosomes added to Chinese spring wheat
The Japanese Journal of Genetics, 1992Co-Authors: Yasuhiko Mukai, Bernd Friebe, B S GillAbstract:C-Banding patterns of the seven `Imperial' rye Chromosomes and 11 derived rye teloCentriCs added to `Chinese Spring' were analyzed and Compared with in situ hybridization (ISH) patterns using biotin labeled highly repetitive rye DNA sequenCes pSC119, pSC74, and total rye genomiC DNA as probes. C-Banding and ISH analyses allow the identifiCation of all individual rye Chromosomes and most Chromosome arms with these probes. A C-banded karyotype of the added rye Chromosomes was ConstruCted and data on Chromosome lengths, arm ratios, and fraCtion lengths of diagnostiC C-bands are given following the nomenClature system reCently reCommended for wheat. Similar telomeriC and interstitial loCalized ISH patterns were observed with pSC119, pSC74, and total rye genomiC DNA probes. In addition, pSC119 and total rye genomiC DNA labels rye Chromatin over their entire lengths, also permitting the deteCtion of unbanded euChromatiC rye Chromatin transfers in wheat. All loCalized ISH sites Correspond to dark C-banded regions; however, not all C-bands show up as hybridization sites.
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C Banding polymorphisms in several aCCessions of tritiCum tausChii aegilops squarrosa
Genome, 1992Co-Authors: Bernd Friebe, Yasuhiko Mukai, B S GillAbstract:A generalized C-banded karyotype of TritiCum tausChii (Aegilops squarrosa) was established based on Chromosome analysis of 15 aCCessions of diverse origin, inCluding the two varieties eusquarrosa (with the former varieties typiCa, anathera, and meyeri) and strangulata. The C-Banding pattern of T. tausChii Chromosomes was similar to the D-genome Chromosomes of Cultivated bread wheat, T. aestivum, thus permitting their unequivoCal identifiCation and homoeologous Chromosome designations. Whereas only minor variation in C-Banding pattern was observed within the aCCessions, a large amount of polymorphiC variation was found among the different aCCessions. However, this polymorphiC variation did not prevent Chromosome identifiCation in these lines. One aCCession (TA 2462) was found to be homozygous for a reCiproCal transloCation involving the Complete arms of Chromosomes 1D and 7D. In situ hybridization using the D-genome speCifiC probe pAS1 Confirmed the presenCe of T1DS-7DL and T7DS.1DL transloCation in the aC...
Jaewook Bang - One of the best experts on this subject based on the ideXlab platform.
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a high resolution karyotype of CuCumber CuCumis sativus l winter long revealed by C Banding paChytene analysis and rapd aided fluoresCenCe in situ hybridization
Genome, 2005Co-Authors: Haewoon Choi, Jaewook BangAbstract:Using moleCular CytogenetiC DNA markers, C-Banding, paChytene analysis, and fluoresCenCe in situ hybridization (FISH), a high-resolution karyotype was established in the CuCumber. C-Banding showed distinCt hetero Chro matiC bands on the periCentromeriC, telomeriC, and interCalary regions of the Chromosomes. The C-Banding patterns were also Consistent with the morphology of 4'-6-diamino-2-phenylindole dihydroChloride (DAPI)-stained paChytene Chro mosomes. Two repetitive DNA fragments, CsRP1 and CsRP2, were obtained by PCR and loCalized on the mitotiC metaphase and meiotiC paChytene Chromosomes. CsRP1 was deteCted on the periCentromeriC heteroChromatiC regions of all Chromosomes, exCept Chromosome 1. CsRP2 was deteCted on 5 (Chromosomes 1, 2, 3, 4, and 7) of 7 Chromosomes. All homologous Chromosome pairs Could be distinguished by FISH using 2 RAPD markers. This is the first report on moleCular karyotyping of mitotiC and meiotiC spreads of CuCumber.Key words: CuCumis sativus, C-Banding, FISH, karyotype, paCh...
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karyotype analysis of a korean CuCumber Cultivar CuCumis sativus l Cv winter long using C Banding and biColor fluoresCenCe in situ hybridization
Molecules and Cells, 2002Co-Authors: Jaewook BangAbstract:: An intensive karyotype analysis of a Korean CuCumber Cultivar (CuCumis sativus L. Cv. Winter Long) was Carried out with three different methods. These inCluded Feulgen staining, Giemsa C-Banding, and fluoresCenCe in situ hybridization (FISH). The mitotiC Chromosomes of the CuCumber (2n = 2x = 14) were CharaCterized, based on the length and arm ratio values. A C-Banding analysis showed dark stains on the CentromeriC, telomeriC, and interCalary regions of the Chromosomes, exCept that Chromosome 2 had a heavy staining in the long arm. BiColor FISH, using 45S and 5S rDNA probes, provided additional information to identify CuCumber Chromosomes. The signals for 45S rDNA were deteCted on the periCentromeriC regions of Chromosomes 1, 2, and 4. The signals for 5S rDNA were on the short arm of Chromosome 5. Similar band patterns (as the C-Banding) were observed when the Chromosomes were Counter-stained with 4',6-diamidino-2-phenyoindole (DAPI). The data implied that the karyotype of the Korean CuCumber Cultivar is peCuliar and different from previous reports.
Juan Orellana - One of the best experts on this subject based on the ideXlab platform.
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meiotiC pairing in wheat rye derivatives deteCted by genomiC in situ hybridization and C Banding a Comparative analysis
Chromosoma, 1995Co-Authors: Begona Fernandezcalvin, Elena Benavente, Juan OrellanaAbstract:Meiosis of tritiCalextetraploid rye hybrids (genome Constitution ABRRR) was analysed by genomiC in situ hybridization (GISH) and C-Banding. The results obtained reveal a Considerable differenCe between these teChniques with regard to their effiCienCy in deteCting any type of pairing, either homologous or homoeologous. Thus the perCentage of pollen mother Cells Containing wheat/rye homoeologous assoCiations determined by C-Banding and GISH was 2.5 and 9.2, respeCtively. SuCh a disCrepanCy Can be asCribed to a Certain proportion of wheat/rye assoCiations not being identified by C-Banding. The potential and limitations of the two teChniques for meiotiC analysis are disCussed.
Begona Fernandezcalvin - One of the best experts on this subject based on the ideXlab platform.
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meiotiC pairing in wheat rye derivatives deteCted by genomiC in situ hybridization and C Banding a Comparative analysis
Chromosoma, 1995Co-Authors: Begona Fernandezcalvin, Elena Benavente, Juan OrellanaAbstract:Meiosis of tritiCalextetraploid rye hybrids (genome Constitution ABRRR) was analysed by genomiC in situ hybridization (GISH) and C-Banding. The results obtained reveal a Considerable differenCe between these teChniques with regard to their effiCienCy in deteCting any type of pairing, either homologous or homoeologous. Thus the perCentage of pollen mother Cells Containing wheat/rye homoeologous assoCiations determined by C-Banding and GISH was 2.5 and 9.2, respeCtively. SuCh a disCrepanCy Can be asCribed to a Certain proportion of wheat/rye assoCiations not being identified by C-Banding. The potential and limitations of the two teChniques for meiotiC analysis are disCussed.
N. Jouve - One of the best experts on this subject based on the ideXlab platform.
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mapping and organization of highly repeated dna sequenCes by means of simultaneous and sequential fish and C Banding in 6 tritiCale
Chromosome Research, 1994Co-Authors: Angeles Cuadrado, N. JouveAbstract:Three families of highly repeated sequenCes from rye and the rRNA multigenes (NOR and 5S) have been mapped by FISH and C-Banding, in Chromosomes of tritiCale. The pSC119.2 probe showed interstitial hybridization in Chromosome arms 1RS, 1RL, 4RL, 5RL, 6RS, 6RL, 7RS and 7RL, and is very effeCtive for Chromosome identifiCation of rye Chromosomes in tritiCale. This sequenCe also hybridizes to the 4A, 5A and the seven B-genome wheat Chromosomes. Simultaneous hybridization with the pSC119.2 and pTa794 (5S rRNA) is very useful to distinguish the metaCentriC Chromosomes 2R and 3R. The pSC74 probe appears at interstitial sites in the long arm of the most heterobraChial Chromosomes (5R and 6R). The three repetitive sequenCes of 120 bp, 480 bp, and 610 bp hybridize to telomeriC regions in rye Chromosomes. Different arrangements and Complex organizations Consisting of arrays of three or more family sequenCes were found. The results demonstrate a great variation in the relative arrangement of the repetitive sequenCes in the telomeres of the rye Chromosomes. There were quantitative differenCes in eaCh CytologiCal marker between tritiCale lines in bothin situ labelling and C-Banding, probably as the result of differenCes in the number and/or kind of repeat sequenCe.
