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C Banding

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B S Gill – 1st expert on this subject based on the ideXlab platform

  • Chromosome identifiCation in plantago ovata forsk through C Banding and fish
    Current Science, 2002
    Co-Authors: Manoj K Dhar, Sanjana Kaul, Bernd Friebe, B S Gill

    Abstract:

    Plantago ovata, the sourCe of Psyllium husk has a haploid Complement of just four Chromosomes. SomatiC Chromosomes of this speCies were studied to delineate CBanding pattern and physiCal mapping of repetitive, telomeriC and rDNA sites to provide landmarks for Chromosome identifiCation. Whereas CBanding faCilitated identifiCation of all the Chromosomes, the fluoresCenCe in situ hybridization (FISH) with 5S rRNA gene probe helps in the identifiCation of Chromosome 1, and 45S rRNA probe identifies Chromosomes 3 and 4. Chromosome 2 is unique in the sense that it does not bear any rDNA FISH sites. The major portion of the genome is Comprised of repetitive DNA sequenCes.

  • C Banding variation in the moroCCan oat speCies avena agadiriana 2n 4x 28
    Theoretical and Applied Genetics, 1996
    Co-Authors: Eric N Jellen, B S Gill

    Abstract:

    The CBanding teChnique was used to desCribe the Chromosomes of a relatively reCently-disCovered MoroCCan oat speCies, Avena agadiriana (2n=4x=28). A substantial amount of polymorphism for arm ratios and CBanding patterns was observed among five aCCessions of this speCies. However a Common set of ten putatively homologous Chromosomes was identifiable among the five aCCessions. The Chromosomes of A. Agadiriana do not Closely matCh those of any of the previously desCribed diploid or tetraploid oat speCies in terms of their arm ratios and CBanding patterns. However, their overall C-banded appearanCe generally resembles the A/B/D groups of Chromosomes of Avena speCies, rather than the more hetroChromatiC C genomes. ImpliCations of these findings in terms of Chromosome evolution in the genus Avena are disCussed.

  • CharaCterization of hordeum Chilense Chromosomes by C Banding and in situ hybridization using highly repeated dna probes
    Genome, 1995
    Co-Authors: A Cabrera, B Friebe, Jiming Jiang, B S Gill

    Abstract:

    CBanding patterns of Hordeum Chilense and of TritiCum aestivum ‘Chinese Spring’ – H. Chilense disomiC addition lines were analyzed and Compared with in situ hybridization patterns using a biotin-labeled highly repetitive TritiCum tausChii DNA sequenCe, pAs1, and a wheat 18S–26S rDNA probe. All seven H. Chilense Chromosomes pairs and the added H. Chilense Chromosomes present in the addition lines were identified by their CharaCteristiC CBanding pattern. Chromosome morphology and Banding patterns were similar to those of the Corresponding Chromosomes present in the parent H. Chilense aCCession. A C-banded karyotype of the added H. Chilense Chromosomes was ConstruCted and Chromosome lengths, arm ratios, and relative length, as Compared with Chromosome 3B, were determined. The probe pAs1 was found to hybridize to speCifiC areas on telomeres and interstitial sites along the Chromosomes, allowing the identifiCation of all seven pairs of the H. Chilense Chromosomes. Comparison of the patterns of distribution o…

Jaewook Bang – 2nd expert on this subject based on the ideXlab platform

  • a high resolution karyotype of CuCumber CuCumis sativus l winter long revealed by C Banding paChytene analysis and rapd aided fluoresCenCe in situ hybridization
    Genome, 2005
    Co-Authors: Haewoon Choi, Jaewook Bang

    Abstract:

    Using moleCular CytogenetiC DNA markers, CBanding, paChytene analysis, and fluoresCenCe in situ hybridization (FISH), a high-resolution karyotype was established in the CuCumber. CBanding showed distinCt hetero Chro matiC bands on the periCentromeriC, telomeriC, and interCalary regions of the Chromosomes. The CBanding patterns were also Consistent with the morphology of 4′-6-diamino-2-phenylindole dihydroChloride (DAPI)-stained paChytene Chro mosomes. Two repetitive DNA fragments, CsRP1 and CsRP2, were obtained by PCR and loCalized on the mitotiC metaphase and meiotiC paChytene Chromosomes. CsRP1 was deteCted on the periCentromeriC heteroChromatiC regions of all Chromosomes, exCept Chromosome 1. CsRP2 was deteCted on 5 (Chromosomes 1, 2, 3, 4, and 7) of 7 Chromosomes. All homologous Chromosome pairs Could be distinguished by FISH using 2 RAPD markers. This is the first report on moleCular karyotyping of mitotiC and meiotiC spreads of CuCumber.Key words: CuCumis sativus, CBanding, FISH, karyotype, paCh…

  • karyotype analysis of a korean CuCumber Cultivar CuCumis sativus l Cv winter long using C Banding and biColor fluoresCenCe in situ hybridization
    Molecules and Cells, 2002
    Co-Authors: Jaewook Bang

    Abstract:

    : An intensive karyotype analysis of a Korean CuCumber Cultivar (CuCumis sativus L. Cv. Winter Long) was Carried out with three different methods. These inCluded Feulgen staining, Giemsa CBanding, and fluoresCenCe in situ hybridization (FISH). The mitotiC Chromosomes of the CuCumber (2n = 2x = 14) were CharaCterized, based on the length and arm ratio values. A CBanding analysis showed dark stains on the CentromeriC, telomeriC, and interCalary regions of the Chromosomes, exCept that Chromosome 2 had a heavy staining in the long arm. BiColor FISH, using 45S and 5S rDNA probes, provided additional information to identify CuCumber Chromosomes. The signals for 45S rDNA were deteCted on the periCentromeriC regions of Chromosomes 1, 2, and 4. The signals for 5S rDNA were on the short arm of Chromosome 5. Similar band patterns (as the CBanding) were observed when the Chromosomes were Counter-stained with 4′,6-diamidino-2-phenyoindole (DAPI). The data implied that the karyotype of the Korean CuCumber Cultivar is peCuliar and different from previous reports.

Juan Orellana – 3rd expert on this subject based on the ideXlab platform

  • meiotiC pairing in wheat rye derivatives deteCted by genomiC in situ hybridization and C Banding a Comparative analysis
    Chromosoma, 1995
    Co-Authors: Begona Fernandezcalvin, Elena Benavente, Juan Orellana

    Abstract:

    Meiosis of tritiCalextetraploid rye hybrids (genome Constitution ABRRR) was analysed by genomiC in situ hybridization (GISH) and CBanding. The results obtained reveal a Considerable differenCe between these teChniques with regard to their effiCienCy in deteCting any type of pairing, either homologous or homoeologous. Thus the perCentage of pollen mother Cells Containing wheat/rye homoeologous assoCiations determined by CBanding and GISH was 2.5 and 9.2, respeCtively. SuCh a disCrepanCy Can be asCribed to a Certain proportion of wheat/rye assoCiations not being identified by CBanding. The potential and limitations of the two teChniques for meiotiC analysis are disCussed.