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Uwe Ritter - One of the best experts on this subject based on the ideXlab platform.
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complexation with C60 Fullerene increases doxorubicin efficiency against leukemic cells in vitro
Nanoscale Research Letters, 2019Co-Authors: S V Prylutska, Yu I Prylutskyy, Uwe Ritter, Anna Grebinyk, Sergii Grebinyk, O. P. Matyshevska, Thomas Dandekar, Marcus FrohmeAbstract:Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 Fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells. Here, we studied the physicochemical properties and anticancer activity of C60 Fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 Fullerene considerable nanocarrier function. The results of this study indicated that C60 Fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells.
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A Novel Nanoconjugate of Landomycin A with C60 Fullerene for Cancer Targeted Therapy: In Vitro Studies.
Cellular and molecular bioengineering, 2018Co-Authors: V. Bilobrov, S V Prylutska, Yu I Prylutskyy, O O Litsis, M P Evstigneev, Viktoriya Sokolova, R. R. Panchuk, V. L. Osetskyi, Matthias Epple, Uwe RitterAbstract:Landomycins are a subgroup of angucycline antibiotics that are produced by Streptomyces bacteria and possess strong antineoplastic potential. Literature data suggest that enhancement of the therapeutic activity of this drug may be achieved by means of creating specific drug delivery systems. Here we propose to adopt C60 Fullerene as flexible and stable nanocarrier for landomycin delivery into tumor cells. The methods of molecular modelling, dynamic light scattering and Fourier transform infrared spectroscopy were used to study the assembly of C60 Fullerene and the anticancer drug Landomycin A (LA) in aqueous solution. Cytotoxic activity of this nanocomplex was studied in vitro towards two cancer cell lines in comparison to human mesenchymal stem cells (hMSCs) using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test and a live/dead assay. The morphology of the cells incubated with Fullerene–drug nanoparticles and their uptake into target cells were studied by scanning electron microscopy and fluorescence light microscopy. The viability of primary cells (hMSCs, as a model for healthy cells) and cancer cell lines (human osteosarcoma cells, MG-63, and mouse mammary cells, 4T1, as models for cancer cells) was studied after incubation with water-soluble C60 Fullerenes, LA and the mixture C60 + LA. The C60 + LA nanocomplex in contrast to LA alone showed higher toxicity towards cancer cells and lower toxicity towards normal cells, whereas the water-soluble C60 Fullerenes at the same concentration were not toxic for the cells. The obtained physico-chemical data indicate a complexation between the two compounds, leading to the formation of a C60 + LA nanocomposite. It was concluded that immobilization of LA on C60 Fullerene enhances selectivity of action of this anticancer drug in vitro, indicating on possibility of further preclinical studies of novel C60 + LA nanocomposites on animal tumor models.
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comparative analysis of the antineoplastic activity of C60 Fullerene with 5 fluorouracil and pyrrole derivative in vivo
Nanoscale Research Letters, 2017Co-Authors: O V Lynchak, Yu I Prylutskyy, O A Kyzyma, M P Evstigneev, V V Kostjukov, V K Rybalchenko, Dmytro Soloviov, Uwe RitterAbstract:The antitumor activity of pristine C60 Fullerene aqueous solution (C60FAS) compared to 5-fluorouracil (5-FU) and pyrrole derivative 1-(4-Cl-benzyl)-3-Cl-4-(CF3-fenylamino)-1H-pyrrol-2.5-dione (MI-1) cytostatic drugs was investigated and analyzed in detail using the model of colorectal cancer induced by 1.2-dimethylhydrazine (DMH) in rats. The number, size, and location of the tumors were measured, and the pathology was examined. It was found that the number of tumors and total lesion area decreased significantly under the action of C60FAS and MI-1. Because these drugs have different mechanisms of action, their simultaneous administration can potentially increase the effectiveness and significantly reduce the side effects of antitumor therapy.
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on the origin of C60 Fullerene solubility in aqueous solution
Langmuir, 2014Co-Authors: Yu I Prylutskyy, V I Petrenko, O I Ivankov, O A Kyzyma, L A Bulavin, O O Litsis, M P Evstigneev, V V Cherepanov, A G Naumovets, Uwe RitterAbstract:In this work, we report that the surface hydroxylation of C60 molecules is the most likely mechanism for pristine C60 Fullerenes/C60 Fullerene aggregate stabilization in water, being independent of the method of C60 Fullerene aqueous solution preparation.
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C60 Fullerene aggregation in aqueous solution
Physical Chemistry Chemical Physics, 2013Co-Authors: Yu I Prylutskyy, Uwe Ritter, Anatoly S Buchelnikov, Dmitry P Voronin, V V Kostjukov, John Parkinson, M P EvstigneevAbstract:In the present work we develop a novel approach for quantification of the energetics of C60 Fullerene aggregation in aqueous media in terms of equilibrium aggregation constant KF. In particular, it is shown that the experimental determination of the magnitude of KF is possible only within the framework of the ‘up-scaled aggregation model’, considering the C60 Fullerene water solution as a solution of Fullerene clusters. Using dynamic light scattering (DLS) data we report the value, KF = 56 000 M−1, which is in good agreement with existing theoretical estimates and the results of energetic analyses. It is suggested that the proposed ‘up-scaled model’ may be used in any instances of non-specific aggregation resulting in formation of large spherical particles. The measurement of the translational diffusion coefficient and the dimensions of the light scattering particles using a DLS approach with respect to C60 Fullerene aggregates is found to contain significant systematic errors originating from the interaction effect that is well-known for micellar solutions. As a result, corrections to the equations associated with DLS data are proposed.
Yu I Prylutskyy - One of the best experts on this subject based on the ideXlab platform.
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complexation with C60 Fullerene increases doxorubicin efficiency against leukemic cells in vitro
Nanoscale Research Letters, 2019Co-Authors: S V Prylutska, Yu I Prylutskyy, Uwe Ritter, Anna Grebinyk, Sergii Grebinyk, O. P. Matyshevska, Thomas Dandekar, Marcus FrohmeAbstract:Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 Fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells. Here, we studied the physicochemical properties and anticancer activity of C60 Fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 Fullerene considerable nanocarrier function. The results of this study indicated that C60 Fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells.
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A Novel Nanoconjugate of Landomycin A with C60 Fullerene for Cancer Targeted Therapy: In Vitro Studies.
Cellular and molecular bioengineering, 2018Co-Authors: V. Bilobrov, S V Prylutska, Yu I Prylutskyy, O O Litsis, M P Evstigneev, Viktoriya Sokolova, R. R. Panchuk, V. L. Osetskyi, Matthias Epple, Uwe RitterAbstract:Landomycins are a subgroup of angucycline antibiotics that are produced by Streptomyces bacteria and possess strong antineoplastic potential. Literature data suggest that enhancement of the therapeutic activity of this drug may be achieved by means of creating specific drug delivery systems. Here we propose to adopt C60 Fullerene as flexible and stable nanocarrier for landomycin delivery into tumor cells. The methods of molecular modelling, dynamic light scattering and Fourier transform infrared spectroscopy were used to study the assembly of C60 Fullerene and the anticancer drug Landomycin A (LA) in aqueous solution. Cytotoxic activity of this nanocomplex was studied in vitro towards two cancer cell lines in comparison to human mesenchymal stem cells (hMSCs) using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test and a live/dead assay. The morphology of the cells incubated with Fullerene–drug nanoparticles and their uptake into target cells were studied by scanning electron microscopy and fluorescence light microscopy. The viability of primary cells (hMSCs, as a model for healthy cells) and cancer cell lines (human osteosarcoma cells, MG-63, and mouse mammary cells, 4T1, as models for cancer cells) was studied after incubation with water-soluble C60 Fullerenes, LA and the mixture C60 + LA. The C60 + LA nanocomplex in contrast to LA alone showed higher toxicity towards cancer cells and lower toxicity towards normal cells, whereas the water-soluble C60 Fullerenes at the same concentration were not toxic for the cells. The obtained physico-chemical data indicate a complexation between the two compounds, leading to the formation of a C60 + LA nanocomposite. It was concluded that immobilization of LA on C60 Fullerene enhances selectivity of action of this anticancer drug in vitro, indicating on possibility of further preclinical studies of novel C60 + LA nanocomposites on animal tumor models.
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comparative analysis of the antineoplastic activity of C60 Fullerene with 5 fluorouracil and pyrrole derivative in vivo
Nanoscale Research Letters, 2017Co-Authors: O V Lynchak, Yu I Prylutskyy, O A Kyzyma, M P Evstigneev, V V Kostjukov, V K Rybalchenko, Dmytro Soloviov, Uwe RitterAbstract:The antitumor activity of pristine C60 Fullerene aqueous solution (C60FAS) compared to 5-fluorouracil (5-FU) and pyrrole derivative 1-(4-Cl-benzyl)-3-Cl-4-(CF3-fenylamino)-1H-pyrrol-2.5-dione (MI-1) cytostatic drugs was investigated and analyzed in detail using the model of colorectal cancer induced by 1.2-dimethylhydrazine (DMH) in rats. The number, size, and location of the tumors were measured, and the pathology was examined. It was found that the number of tumors and total lesion area decreased significantly under the action of C60FAS and MI-1. Because these drugs have different mechanisms of action, their simultaneous administration can potentially increase the effectiveness and significantly reduce the side effects of antitumor therapy.
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on the origin of C60 Fullerene solubility in aqueous solution
Langmuir, 2014Co-Authors: Yu I Prylutskyy, V I Petrenko, O I Ivankov, O A Kyzyma, L A Bulavin, O O Litsis, M P Evstigneev, V V Cherepanov, A G Naumovets, Uwe RitterAbstract:In this work, we report that the surface hydroxylation of C60 molecules is the most likely mechanism for pristine C60 Fullerenes/C60 Fullerene aggregate stabilization in water, being independent of the method of C60 Fullerene aqueous solution preparation.
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COMBINED EFFECT OF ELECTROMAGNETIC RADIATION, DNA-INTERCALATORS, C60-Fullerene AND CAFFEINE ON HUMAN BUCCAL EPITHELIUM CELLS
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine., 2014Co-Authors: G. B. Skamrova, Yu I Prylutskyy, M P EvstigneevAbstract:Now the number of physical and chemical biologically active damage factors dramatically increased. The ways to neutralize such effects have not been studied enough. In this work the techniques of visual assessment of chromatin granulation and of electronegativity of human buccal epithelium cell nuclei were used in order to study the combined effects of the exposure to low-intensity electromagnetic radiation of the millimeter range electromagnetic radiation and to the DNA-binding compounds, such as: antibiotic doxorubicin, mutagens ethidium bromide and proflavine, as well as to caffeine and C60 -Fullerene which are not directly interact with DNA. When the action of electromagnetic radiation and DNA-binding compounds is combined, a synergistic effect of reducing the cell response was observed in contrast to the effects caused by electromagnetic radiation and drugs separately. When cells were irradiated in the presence of C60 -Fullerene or caffeine, a protective effect of compounds against electromagnetic radiation influence was observed. The obtained results may provide perspectives in the use of the C60 Fullerene and caffeine as DNA-protectors under the action of electromagnetic radiation
M P Evstigneev - One of the best experts on this subject based on the ideXlab platform.
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A Novel Nanoconjugate of Landomycin A with C60 Fullerene for Cancer Targeted Therapy: In Vitro Studies.
Cellular and molecular bioengineering, 2018Co-Authors: V. Bilobrov, S V Prylutska, Yu I Prylutskyy, O O Litsis, M P Evstigneev, Viktoriya Sokolova, R. R. Panchuk, V. L. Osetskyi, Matthias Epple, Uwe RitterAbstract:Landomycins are a subgroup of angucycline antibiotics that are produced by Streptomyces bacteria and possess strong antineoplastic potential. Literature data suggest that enhancement of the therapeutic activity of this drug may be achieved by means of creating specific drug delivery systems. Here we propose to adopt C60 Fullerene as flexible and stable nanocarrier for landomycin delivery into tumor cells. The methods of molecular modelling, dynamic light scattering and Fourier transform infrared spectroscopy were used to study the assembly of C60 Fullerene and the anticancer drug Landomycin A (LA) in aqueous solution. Cytotoxic activity of this nanocomplex was studied in vitro towards two cancer cell lines in comparison to human mesenchymal stem cells (hMSCs) using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) test and a live/dead assay. The morphology of the cells incubated with Fullerene–drug nanoparticles and their uptake into target cells were studied by scanning electron microscopy and fluorescence light microscopy. The viability of primary cells (hMSCs, as a model for healthy cells) and cancer cell lines (human osteosarcoma cells, MG-63, and mouse mammary cells, 4T1, as models for cancer cells) was studied after incubation with water-soluble C60 Fullerenes, LA and the mixture C60 + LA. The C60 + LA nanocomplex in contrast to LA alone showed higher toxicity towards cancer cells and lower toxicity towards normal cells, whereas the water-soluble C60 Fullerenes at the same concentration were not toxic for the cells. The obtained physico-chemical data indicate a complexation between the two compounds, leading to the formation of a C60 + LA nanocomposite. It was concluded that immobilization of LA on C60 Fullerene enhances selectivity of action of this anticancer drug in vitro, indicating on possibility of further preclinical studies of novel C60 + LA nanocomposites on animal tumor models.
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comparative analysis of the antineoplastic activity of C60 Fullerene with 5 fluorouracil and pyrrole derivative in vivo
Nanoscale Research Letters, 2017Co-Authors: O V Lynchak, Yu I Prylutskyy, O A Kyzyma, M P Evstigneev, V V Kostjukov, V K Rybalchenko, Dmytro Soloviov, Uwe RitterAbstract:The antitumor activity of pristine C60 Fullerene aqueous solution (C60FAS) compared to 5-fluorouracil (5-FU) and pyrrole derivative 1-(4-Cl-benzyl)-3-Cl-4-(CF3-fenylamino)-1H-pyrrol-2.5-dione (MI-1) cytostatic drugs was investigated and analyzed in detail using the model of colorectal cancer induced by 1.2-dimethylhydrazine (DMH) in rats. The number, size, and location of the tumors were measured, and the pathology was examined. It was found that the number of tumors and total lesion area decreased significantly under the action of C60FAS and MI-1. Because these drugs have different mechanisms of action, their simultaneous administration can potentially increase the effectiveness and significantly reduce the side effects of antitumor therapy.
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on the origin of C60 Fullerene solubility in aqueous solution
Langmuir, 2014Co-Authors: Yu I Prylutskyy, V I Petrenko, O I Ivankov, O A Kyzyma, L A Bulavin, O O Litsis, M P Evstigneev, V V Cherepanov, A G Naumovets, Uwe RitterAbstract:In this work, we report that the surface hydroxylation of C60 molecules is the most likely mechanism for pristine C60 Fullerenes/C60 Fullerene aggregate stabilization in water, being independent of the method of C60 Fullerene aqueous solution preparation.
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COMBINED EFFECT OF ELECTROMAGNETIC RADIATION, DNA-INTERCALATORS, C60-Fullerene AND CAFFEINE ON HUMAN BUCCAL EPITHELIUM CELLS
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine., 2014Co-Authors: G. B. Skamrova, Yu I Prylutskyy, M P EvstigneevAbstract:Now the number of physical and chemical biologically active damage factors dramatically increased. The ways to neutralize such effects have not been studied enough. In this work the techniques of visual assessment of chromatin granulation and of electronegativity of human buccal epithelium cell nuclei were used in order to study the combined effects of the exposure to low-intensity electromagnetic radiation of the millimeter range electromagnetic radiation and to the DNA-binding compounds, such as: antibiotic doxorubicin, mutagens ethidium bromide and proflavine, as well as to caffeine and C60 -Fullerene which are not directly interact with DNA. When the action of electromagnetic radiation and DNA-binding compounds is combined, a synergistic effect of reducing the cell response was observed in contrast to the effects caused by electromagnetic radiation and drugs separately. When cells were irradiated in the presence of C60 -Fullerene or caffeine, a protective effect of compounds against electromagnetic radiation influence was observed. The obtained results may provide perspectives in the use of the C60 Fullerene and caffeine as DNA-protectors under the action of electromagnetic radiation
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C60 Fullerene aggregation in aqueous solution
Physical Chemistry Chemical Physics, 2013Co-Authors: Yu I Prylutskyy, Uwe Ritter, Anatoly S Buchelnikov, Dmitry P Voronin, V V Kostjukov, John Parkinson, M P EvstigneevAbstract:In the present work we develop a novel approach for quantification of the energetics of C60 Fullerene aggregation in aqueous media in terms of equilibrium aggregation constant KF. In particular, it is shown that the experimental determination of the magnitude of KF is possible only within the framework of the ‘up-scaled aggregation model’, considering the C60 Fullerene water solution as a solution of Fullerene clusters. Using dynamic light scattering (DLS) data we report the value, KF = 56 000 M−1, which is in good agreement with existing theoretical estimates and the results of energetic analyses. It is suggested that the proposed ‘up-scaled model’ may be used in any instances of non-specific aggregation resulting in formation of large spherical particles. The measurement of the translational diffusion coefficient and the dimensions of the light scattering particles using a DLS approach with respect to C60 Fullerene aggregates is found to contain significant systematic errors originating from the interaction effect that is well-known for micellar solutions. As a result, corrections to the equations associated with DLS data are proposed.
Marinella Farré - One of the best experts on this subject based on the ideXlab platform.
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bioconcentration and bioaccumulation of C60 Fullerene and C60 epoxide in biofilms and freshwater snails radix sp
Environmental Research, 2020Co-Authors: Josep Sanchís, Damià Barceló, Anna Freixa, Julio Cesar Lopezdoval, Lucia H M L M Santos, Sergi Sabater, Esteban Abad, Marinella FarréAbstract:Abstract Fullerenes are carbon nanomaterials that have awaken a strong interest due to their adsorption properties and potential applications in many fields. However, there are some gaps of information about their effects and bioconcentration potential in the aquatic biota. In the present work, freshwater biofilms and snails (Radix sp.) were exposed to Fullerene C60 aggregates, at concentrations in the low μg/L order, in mesocosms specifically designed to mimic the conditions of a natural stream. The bioconcentration factors of C60 Fullerene and its main transformation product, [6,6]C60O epoxide, were studied to the mentioned organisms employing analyses by liquid chromatography coupled to high-resolution mass spectrometry. Our results show that C60 Fullerene and its [6,6]C60O present a low bioconcentration factor (BCF) to biofilms: BCFC60 = 1.34 ± 0.95 L/kgdw and BCFC60O = 1.43 ± 0.72 L/kgdw. This suggests that the sorption of these aggregates to biota may be less favoured than it would be suggested by its hydrophobic character. According to our model, the surface of Fullerene aggregates is saturated with [6,6]C60O molecules, which exposes the polar epoxide moieties in the surface of the aggregates and decreases their affinity to biofilms. In contrast, freshwater snails showed a moderate capacity to actively retain C60 Fullerenes in their organism (BAFC60 = 2670 ± 3070 L/kgdw; BAFC60O = 1330 ± 1680 L/kgdw), probably through ingestion. Our results indicate that the bioaccumulation of these carbon nanomaterials can be hardly estimated using their respective octanol-water partition coefficients, and that their colloidal properties, as well as the feeding strategies of the tested organism, play fundamental roles.
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Quantitative trace analysis of Fullerenes in river sediment from Spain and soils from Saudi Arabia.
Analytical and bioanalytical chemistry, 2013Co-Authors: Josep Sanchís, Marinella Farré, Dalibor Božović, Naif Abdullah Al-harbi, Luis F.o. Silva, Damià BarcelóAbstract:A quantitative method based on ultrasound-assisted toluene extraction followed by liquid chromatography–electrospray ionization–tandem mass spectrometry for the analysis of C60 and C70 Fullerenes, N-methylfulleropyrrolidine, [6, 6]-phenyl C61 butyric acid methyl ester and [6, 6]-thienyl C61 butyric acid methyl ester has been developed. The method was validated using fortified blank river sediments according to the criteria of Commission Decision 2002/657/EC. The method limits of detection ranged from 14 to 290 pg/g, making it suitable for its application in environmental analysis. The method has been applied to investigate Fullerene content in 58 soil samples collected from different urban and industrial areas in Saudi Arabia and in river sediment from six different sites in the Llobregat River Basin. In addition, in the case of the Llobregat River, superficial water samples from the same sites of the sediments were collected and analysed using a previous method. In soils from Saudi Arabia, C60-Fullerene was the only compound that was detected and quantified in 19 % of samples. In the sediments of the Llobregat River, C60-Fullerene was also the only one detected (33 % of the samples), while in river water, C70-Fullerene was the most frequent compound, and it was quantified in 67 % of the samples. However, C60-Fullerene was present in two of the six samples, but at higher concentrations than C70-Fullerene, ranging from 0.9 to 7.8 ng/L.
Anna Grebinyk - One of the best experts on this subject based on the ideXlab platform.
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complexation with C60 Fullerene increases doxorubicin efficiency against leukemic cells in vitro
Nanoscale Research Letters, 2019Co-Authors: S V Prylutska, Yu I Prylutskyy, Uwe Ritter, Anna Grebinyk, Sergii Grebinyk, O. P. Matyshevska, Thomas Dandekar, Marcus FrohmeAbstract:Conventional anticancer chemotherapy is limited because of severe side effects as well as a quickly evolving multidrug resistance of the tumor cells. To address this problem, we have explored a C60 Fullerene-based nanosized system as a carrier for anticancer drugs for an optimized drug delivery to leukemic cells. Here, we studied the physicochemical properties and anticancer activity of C60 Fullerene noncovalent complexes with the commonly used anticancer drug doxorubicin. C60-Doxorubicin complexes in a ratio 1:1 and 2:1 were characterized with UV/Vis spectrometry, dynamic light scattering, and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The obtained analytical data indicated that the 140-nm complexes were stable and could be used for biological applications. In leukemic cell lines (CCRF-CEM, Jurkat, THP1 and Molt-16), the nanocomplexes revealed ≤ 3.5 higher cytotoxic potential in comparison with the free drug in a range of nanomolar concentrations. Also, the intracellular drug’s level evidenced C60 Fullerene considerable nanocarrier function. The results of this study indicated that C60 Fullerene-based delivery nanocomplexes had a potential value for optimization of doxorubicin efficiency against leukemic cells.
