The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Ivančica Trošić - One of the best experts on this subject based on the ideXlab platform.
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The Effect of Cadmium Chloride and Vitamin C on DNA Damage in V79 Cells
2004Co-Authors: Vilena Kašuba, Ružica Rozgaj, Ivančica TrošićAbstract:In the present work, the ability of Vitamin C to modulate genotoxic effects of Cadmium Chloride on Chinese hamster V79 cells was assessed using alkaline single cell gel electrophoresis (comet assay). The comet assay detects release of the DNA from a highly supercoiled DNA-protein complex. The extent of migration is proportional to the number of breaks in DNA. Tail length ana tail moment reflect DNA damage. Chinese hamster V79 cells were treated with various concentrations of Cadmium Chloride (10-4 M, 10-5 M, 10 -6 M and 10 – 7 M) for 24 hours, with, or without pre-treatment with 100 microM ascorbic acid three hours before Cadmium Chloride was added. Three separate cultures per concentration were analysed. The tail lengths and tail moments of 100 cells per culture were evaluated. To evaluate statistical significance of the data, one-way analysis of variance was used. In addition, a Tukey Post Hoc comparison of means for unequal N was performed to test statistical significance between groups. Cadmium Chloride increased tail length and tail moment. The results showed significant difference between both supplemented or un-supplemented 10-4 M CdCl2 and all other groups, but there was not difference in the effect between these two groups. Protective role of Vitamin C was not confirmed at all tested concentrations of Cadmium Chloride. Our results showed that the alkaline comet assay might be considered as a good test for measuring early heavy metal DNA damage in Chinese hamster V79 cells.
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509 Genotoxic effects of Cadmium Chloride in V79 cell culture
Toxicology Letters, 2003Co-Authors: Vilena Kašuba, Ružica Rozgaj, Ivančica TrošićAbstract:Cadmium Chloride was tested for the ability to induce genotoxic effects in V79 cell culture. DNA damage induced by different doses of Cadmium Chloride was observed through the frequency of micronuclei in cytochalasin B-blocked micronucleus assay and through DNA migration in the single cell gel electrophoresis (comet assay). Cells were treated with different doses of Cadmium Chloride (10-4 to 10-6 M) 24 hours after seeding. The effects of ascorbic acid (conc. 100 microM) on Cadmium-induced DNA damage was also evaluated. The results were analyzed by one-way ANOVA. The cytochalasin B-blocked micronucleus assay showed that 10-4 to 10-6 M and vitamin C supplemented 10-6 M frequencies of MN significantly differed from the control sample, and that 10-4, 10-5 M and vitamin C supplemented 10-5 M samples were significantly differed from the vitamin C sample. The Comet assay showed that the tail lengths from un-supplemented and vitamin C supplemented 10-4 M sample significantly differed from the control sample ; and that 10-5 M and vitamin C supplemented 10-4 M sample significantly differed from vitamin C sample. Tail moments from un-supplemented 10-4 M and vitamin C supplemented 10-6 M samples significantly differed from the vitamin C sample. Results indicate that Cadmium Chloride increases DNA damages as well as frequency of micronuclei. On the other hand, protective role of vitamin C was not confirmed at all tested concentrations of Cadmium Chloride.
Vilena Kašuba - One of the best experts on this subject based on the ideXlab platform.
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The Effect of Cadmium Chloride and Vitamin C on DNA Damage in V79 Cells
2004Co-Authors: Vilena Kašuba, Ružica Rozgaj, Ivančica TrošićAbstract:In the present work, the ability of Vitamin C to modulate genotoxic effects of Cadmium Chloride on Chinese hamster V79 cells was assessed using alkaline single cell gel electrophoresis (comet assay). The comet assay detects release of the DNA from a highly supercoiled DNA-protein complex. The extent of migration is proportional to the number of breaks in DNA. Tail length ana tail moment reflect DNA damage. Chinese hamster V79 cells were treated with various concentrations of Cadmium Chloride (10-4 M, 10-5 M, 10 -6 M and 10 – 7 M) for 24 hours, with, or without pre-treatment with 100 microM ascorbic acid three hours before Cadmium Chloride was added. Three separate cultures per concentration were analysed. The tail lengths and tail moments of 100 cells per culture were evaluated. To evaluate statistical significance of the data, one-way analysis of variance was used. In addition, a Tukey Post Hoc comparison of means for unequal N was performed to test statistical significance between groups. Cadmium Chloride increased tail length and tail moment. The results showed significant difference between both supplemented or un-supplemented 10-4 M CdCl2 and all other groups, but there was not difference in the effect between these two groups. Protective role of Vitamin C was not confirmed at all tested concentrations of Cadmium Chloride. Our results showed that the alkaline comet assay might be considered as a good test for measuring early heavy metal DNA damage in Chinese hamster V79 cells.
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509 Genotoxic effects of Cadmium Chloride in V79 cell culture
Toxicology Letters, 2003Co-Authors: Vilena Kašuba, Ružica Rozgaj, Ivančica TrošićAbstract:Cadmium Chloride was tested for the ability to induce genotoxic effects in V79 cell culture. DNA damage induced by different doses of Cadmium Chloride was observed through the frequency of micronuclei in cytochalasin B-blocked micronucleus assay and through DNA migration in the single cell gel electrophoresis (comet assay). Cells were treated with different doses of Cadmium Chloride (10-4 to 10-6 M) 24 hours after seeding. The effects of ascorbic acid (conc. 100 microM) on Cadmium-induced DNA damage was also evaluated. The results were analyzed by one-way ANOVA. The cytochalasin B-blocked micronucleus assay showed that 10-4 to 10-6 M and vitamin C supplemented 10-6 M frequencies of MN significantly differed from the control sample, and that 10-4, 10-5 M and vitamin C supplemented 10-5 M samples were significantly differed from the vitamin C sample. The Comet assay showed that the tail lengths from un-supplemented and vitamin C supplemented 10-4 M sample significantly differed from the control sample ; and that 10-5 M and vitamin C supplemented 10-4 M sample significantly differed from vitamin C sample. Tail moments from un-supplemented 10-4 M and vitamin C supplemented 10-6 M samples significantly differed from the vitamin C sample. Results indicate that Cadmium Chloride increases DNA damages as well as frequency of micronuclei. On the other hand, protective role of vitamin C was not confirmed at all tested concentrations of Cadmium Chloride.
Manu Multani - One of the best experts on this subject based on the ideXlab platform.
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Vibrational Spectroscopic Study of the Semiorganic Nonlinear Optical Crystal Bis(thiourea)Cadmium Chloride
Journal of Raman Spectroscopy, 1997Co-Authors: V. Venkataramanan, H. L. Bhat, M. R. Srinivasan, Pushan Ayyub, Manu MultaniAbstract:Polarized Raman and infrared spectra of bis(thiourea)Cadmium Chloride, a novel semiorganic nonlinear optical crystal, were recorded to determine the symmetries of molecular vibrations. The observed Raman and infrared bands are assigned to molecular vibrations of thiourea, Cadmium–sulphur and Cadmium–Chloride bonds. The vibrational spectra are interpreted in the light of crystal structure data.
Fawzia A. E. Aly - One of the best experts on this subject based on the ideXlab platform.
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In vivo and in vitro studies on the genotoxicity of Cadmium Chloride in mice.
Journal of applied toxicology : JAT, 2000Co-Authors: Maha A. Fahmy, Fawzia A. E. AlyAbstract:The genotoxic effect of Cadmium Chloride was evaluated in chromosomes of experimental mice using in vivo and in vitro studies. In vivo the induction of micronuclei, sister chromatid exchange in mouse bone marrow and chromosomal aberrations in both somatic and germ cells was investigated. Doses 1.9, 5.7 and 7.6 mg kg−1 body wt. (single i.p. treatment) induced a significant and dose-dependent increase in the percentage of polychromatic erythrocytes with micronuclei. Such a percentage reached 2.1% with the highest tested dose, compared with 0.57% for the control (non-treated) and 2.2% for mitomycin c as the positive control. The dose of 1.9 mg kg−1 body wt. had no significant effect with respect to sister chromatid exchange (SCE) but the doses of 5.7 and 7.6 mg kg−1body wt. increased the frequency of SCEs significantly. The frequency of SCE reached 7.35 ± 0.26 per cell after treatment with the highest tested dose, which is a less than twofold increase compared with the control frequency of 4.6 ± 0.42 per cell. However mitomycin c induced a much higher effect (12.1 ± 0.73). Cadmium Chloride also induced a significant increase in the percentage of chromosomal aberrations in mouse bone marrow at the doses of 5.7 and 9.5 mg kg−1 body wt. (single i.p. treatment). The effect is a function of Cadmium Chloride concentration. Moreover, Cadmium Chloride induced its maximum effect concerning the induction of chromosomal aberrations in mouse bone marrow cells 24 h after treatment, compared with 12 and 48 h. In germ cells, chromosomal aberrations were observed in mouse spermatocytes 12 days post-treatment with the dose of 5.7 mg kg−1 body wt. Moreover, a pronounced reduction in the number of spermatocytes was observed after administration of Cadmium Chloride (0.9, 1.9 and 5.7 mg kg−1 body wt.) In in vitro studies, the three tested concentrations of 10, 15 and 20 µg ml−1 Cadmium Chloride induced a statistically significant increase in the frequency of SCEs in cultured mouse spleen cells. The concentrations of 15 and 20 µg ml−1 also induced chromosomal aberrations in mouse spleen culture. The ability of vitamin C (l-ascorbic acid) to minimize the incidence of chromosomal aberrations induced by Cadmium Chloride in cultured mouse spleen cells was investigated. Vitamin C at the concentrations of 3 and 6 µg ml−1 significantly minimized the percentage of aberrant cells induced by Cadmium Chloride. Copyright © 2000 John Wiley & Sons, Ltd.
Wang Ziliang - One of the best experts on this subject based on the ideXlab platform.
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Acute toxic effect of Cadmium Chloride in mice
Chinese journal of veterinary science, 2012Co-Authors: Wang ZiliangAbstract:Mice were orally administered with Cadmium Chloride(the control group:0.0 mg/kg;the test groups Ⅰ-Ⅵ:98.3,122.9,153.6,192.0,240.0,300.0 mg/kg,respectively) to carry out acute toxicity test by Bliss method,and then these indexes such as death rate,body weight,organ relative index,blood physiological index,necropsy and histopathology were analyzed.The results showed that the LD50 of Cadmium Chloride in mice was 187.03 mg/kg.The body weight gain of mice was cut down with the dosage raising of Cadmium Chloride(P0.01).Compared with the control group,the difference of the cardiac relative index in Ⅰgroup was not significant(P0.05),but it was significant(P0.05) in the other groups,the difference of the testis and the spleen relative index were highly significant(P0.01),and the relative indexes of the rest organs were no significant difference(P0.05).The blood physiological index fluctuated in normal ranges(P0.05).In the dead mice of the each group except that the cerebrum tissue had not obviously pathological changes,the rest tissues all had the pathological changes,especially in Ⅵ group.In the survival mice the pathological changes of the tissues were slighter in Ⅰ group and more severe in Ⅴ group than other groups.The results suggested that Cadmium Chloride was the medium intensity toxic substance and it could produce the different degree acute toxic effects in some tissues of mice.
