The Experts below are selected from a list of 1971 Experts worldwide ranked by ideXlab platform
W P Saunders - One of the best experts on this subject based on the ideXlab platform.
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comparative evaluation of Calcium Hypochlorite and sodium Hypochlorite on soft tissue dissolution
Journal of Endodontics, 2012Co-Authors: Arindam Dutta, W P SaundersAbstract:INTRODUCTION: The aim of this study was to compare in vitro the tissue-dissolution properties of 5% and 10% Calcium Hypochlorite (Ca(OCl)(2)) with two concentrations (1.36% and 4.65%) of proprietary sodium Hypochlorite (NaOCl) on bovine muscle tissue. METHODS: The available chlorine concentration of each solution was determined using iodometric titration. Tissue specimens from bovine muscle were weight adjusted (50 ± 5 mg). Ten tissue specimens in each group were immersed in 5 mL each test solution, removed after 5 minutes, blotted dry, and weighed. The process was repeated every 5 minutes with a fresh 5-mL aliquot of the test solution for 60 minutes or until complete tissue dissolution, whichever was quickest. The percentage weight loss of the specimens was calculated over the experimental period. RESULTS: Available chlorine concentrations of the irrigants ranged from 1.36% to 4.65%. All solutions dissolved tissue completely after 60 minutes except 5% Ca(OCl)(2) (99.4% dissolution). Between the 35- and 60-minute test readings, there were no significant differences between the solutions. Chlorax (4.65% NaOCl) (Cerkamed Group, Nisko, Poland) dissolved tissue quicker during the first 35 minutes (P < .05). In this period, the weight loss with 10% Ca(OCl)(2) differed from Chlorax at all time intervals except at 5 and 35 minutes (P < .05); 5% Ca(OCl)(2) showed no significant differences with 10% Ca(OCl)(2) and Tesco bleach (1.36% NaOCl) (Tesco Stores Ltd, Chestnut, UK) in the first 35 minutes except at the 5-minute measurement. CONCLUSIONS: Within the limitations of this study, Chlorax (4.65% NaOCl) dissolved tissue faster than the Ca(OCl)(2) solutions and Tesco thin bleach (1.36% NaOCl) over the first 35 minutes, but there were no significant differences among the solutions thereafter.
Francisco Montagner - One of the best experts on this subject based on the ideXlab platform.
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effect of edta sodium and Calcium Hypochlorite on the inorganic component of root canal dentin a sem analysis
Microscopy Research and Technique, 2019Co-Authors: Luana Roleto Cardoso, Francisco Montagner, Flávia Emi Razera Baldasso, Débora Delai, Patrícia Maria Poli KopperAbstract:: This study evaluated the influence of canal irrigation protocols with 5.25% Calcium Hypochlorite [Ca(OCl)2 ], 5.25% sodium Hypochlorite (NaOCl) and 17% EDTA solution on the inorganic component of root canal dentin. Sixty roots were randomly divided into six groups (n = 10): Saline solution (SS) (control); Saline solution (SSE) +17% EDTA; CH - 5.25% Ca(OCl)2 ; CHE - 5.25% Ca(OCl)2 + 17% EDTA; SH - 5.25% NaOCl; SHE - 5.25% NaOCl +17% EDTA. After canal irrigation, the specimens were longitudinally split and analyzed by scanning electron microscopy (SEM). Dentinal tubules were observed in transverse (middle and apical thirds) direction. The images were classified in scores, according to smear layer removal and peritubular dentin alteration, and data were analyzed by Kruskal-Wallis's test, followed by Dunn's test (α = 0.05). In the middle third, all groups with EDTA presented better removal of the smear layer with higher peritubular dentin alteration (p < .05). In the apical third, this happened only in SHE (p < .05). Ca(OCl)2 and NaOCl exhibit similar performance to remove smear layer and alteration of the inorganic component in the middle third. In the apical third, NaOCl associated with EDTA showed better performance in smear layer removal.
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Calcium Hypochlorite Solutions - An In Vitro Evaluation of Antimicrobial Action and Pulp Dissolution.
European endodontic journal, 2019Co-Authors: Karen Barea De Paula, Israel Bangel Carlotto, Marconi Df, Fabiana Soares Grecca, Francisco MontagnerAbstract:Objective: To compare the antimicrobial activity and tissue dissolution capacity of Calcium Hypochlorite (Ca(OCl)2) solution with sodium Hypochlorite (NaOCl) solution at 0.5%, 1.0%, 2.5%, and 5.25% concentrations. Methods: To determine the inhibition halos produced by the tested substances against Enterococcus faecalis, the agar diffusion method was employed. Additionally, the broth contact method was used to determine the time required for the inhibition of E. faecalis. Bovine pulp fragments were used to test the dissolution. Half of the pulps were freely deposited samples in cell culture wells, and the remaining samples were fixed on bovine dentine bases. Results: For both Ca(OCl)2 and NaOCl solutions, the greatest inhibition zones were observed at 5.25% con-centration. However, the most significant inhibition zone was measured with 5.25% Ca(OCl)2 solution (17.38 mm). Hypochlorite solutions at 2.5% and 5.25% concentrations required less time to inhibit E. faecalis than those at 0.5% and 1.0% concentrations (P 0.05). The most effective Hypochlorite solution concentrations were 5.25% and 2.5% for dissolving pulp fragments (P
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influence of surfactants addition on the properties of Calcium Hypochlorite solutions
Journal of Applied Oral Science, 2019Co-Authors: Julia Eick Iglesias, Francisco Montagner, Lucas Siqueira Pinheiro, Daniel Eduardo Weibel, Fabiana Soares GreccaAbstract:OBJECTIVES: The aim of this study was to evaluate the influence of surfactants 0.2% or 0.1% cetrimide (Cet) or 0.008% benzalkonium chloride (BAK) on 2.5% Calcium Hypochlorite (Ca(OCl)2), and compare to sodium Hypochlorite (NaOCl), regarding the properties of pH, free chlorine content, surface tension, contact angle, pulp dissolution and antimicrobial activity. MATERIAL AND METHODS: The pH and free chlorine content were evaluated by digital pHmeter and by titration, respectively. Surface tension was measured by the platinum ring technique with a Du Nouy tensiometer. The solution's contact angle in human dentin surfaces was checked by Drop Shape Analyzer software. Bovine pulps were used for pulp dissolution analysis and the dissolving capacity was expressed by percent weight loss. Antimicrobial activity over Enterococcus faecalis was evaluated by the agar diffusion method. RESULTS: Surfactants addition to Ca(OCl)2 and NaOCl did not alter the pH, free chlorine content and pulp dissolution properties. Ca(OCl)2 had the highest surface tension among all tested solutions. When surfactants were added to Ca(OCl)2 and NaOCl, there was a significant reduction of surface tension and contact angle values. The addition of 0.2% or 0.1% Cet enhanced antimicrobial activity of both Ca(OCl)2 and NaOCl. CONCLUSION: Surfactant addition to 2.5% Ca(OCl)2 has shown acceptable outcomes for pH, free chlorine content, surface tension, contact angle, pulp dissolution and antimicrobial activity. Furthermore, the addition of 0.2% Cet showed better results for all tested properties.
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Effect of EDTA, sodium, and Calcium Hypochlorite on the inorganic component of root canal dentin: A SEM analysis
Microscopy Research and Technique, 2018Co-Authors: Luana Roleto Cardoso, Francisco Montagner, Flávia Emi Razera Baldasso, Débora Delai, Patrícia Maria Poli KopperAbstract:: This study evaluated the influence of canal irrigation protocols with 5.25% Calcium Hypochlorite [Ca(OCl)2 ], 5.25% sodium Hypochlorite (NaOCl) and 17% EDTA solution on the inorganic component of root canal dentin. Sixty roots were randomly divided into six groups (n = 10): Saline solution (SS) (control); Saline solution (SSE) +17% EDTA; CH - 5.25% Ca(OCl)2 ; CHE - 5.25% Ca(OCl)2 + 17% EDTA; SH - 5.25% NaOCl; SHE - 5.25% NaOCl +17% EDTA. After canal irrigation, the specimens were longitudinally split and analyzed by scanning electron microscopy (SEM). Dentinal tubules were observed in transverse (middle and apical thirds) direction. The images were classified in scores, according to smear layer removal and peritubular dentin alteration, and data were analyzed by Kruskal-Wallis's test, followed by Dunn's test (α = 0.05). In the middle third, all groups with EDTA presented better removal of the smear layer with higher peritubular dentin alteration (p
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Calcium Hypochlorite solutions an in vitro evaluation of antimicrobial action and pulp dissolution
European endodontic journal, 2018Co-Authors: Karen Barea De Paula, Israel Bangel Carlotto, Fabiana Soares Grecca, D F Marconi, Francisco MontagnerAbstract:Objective: To compare the antimicrobial activity and tissue dissolution capacity of Calcium Hypochlorite (Ca(OCl)2) solution with sodium Hypochlorite (NaOCl) solution at 0.5%, 1.0%, 2.5%, and 5.25% concentrations. Methods: To determine the inhibition halos produced by the tested substances against Enterococcus faecalis, the agar diffusion method was employed. Additionally, the broth contact method was used to determine the time required for the inhibition of E. faecalis. Bovine pulp fragments were used to test the dissolution. Half of the pulps were freely deposited samples in cell culture wells, and the remaining samples were fixed on bovine dentine bases. Results: For both Ca(OCl)2 and NaOCl solutions, the greatest inhibition zones were observed at 5.25% con-centration. However, the most significant inhibition zone was measured with 5.25% Ca(OCl)2 solution (17.38 mm). Hypochlorite solutions at 2.5% and 5.25% concentrations required less time to inhibit E. faecalis than those at 0.5% and 1.0% concentrations (P 0.05). The most effective Hypochlorite solution concentrations were 5.25% and 2.5% for dissolving pulp fragments (P<0.05). Additionally, suspended pulp fragments were more susceptible to dissolution than fragments attached to dentine blocks (P<0.05), except for 0.5% Ca(OCl)2. Conclusion: Ca(OCl)2 solutions showed antimicrobial activity against E. faecalis and can dissolve pulp tissues. Future studies are warranted to examine the suitability of Ca(OCl)2 in the chemico-mechanical preparation of the root canal system.
John E. Thoppil - One of the best experts on this subject based on the ideXlab platform.
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Genotoxic assessment of Calcium Hypochlorite and Strychnos potatorum Linn. seeds—two commonly used water purifying agents
Reviews on Environmental Health, 2014Co-Authors: Sandhya Vincent Neelamkavil, John E. ThoppilAbstract:AbstractThe role of water in our daily lives cannot be highlighted enough, and ensuring the availability of pure water is an urgent need. Bleaching powder (Calcium Hypochlorite) and
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Genotoxic assessment of Calcium Hypochlorite and Strychnos potatorum Linn. seeds-two commonly used water purifying agents.
Reviews on environmental health, 2014Co-Authors: Sandhya Vincent Neelamkavil, John E. ThoppilAbstract:: The role of water in our daily lives cannot be highlighted enough, and ensuring the availability of pure water is an urgent need. Bleaching powder (Calcium Hypochlorite) and Strychnos potatorum Linn. seeds are commonly used in water purification as a disinfectant and anticoagulant, respectively, yet their safety levels have not been analyzed so far. Hence, a genotoxic assessment was conducted using Allium cepa chromosome aberration assay. Reduction in mitotic index and increase in abnormality percentage was observed for both, but this effect was dose dependent. All values were statistically significant at p
Shubhra Malik - One of the best experts on this subject based on the ideXlab platform.
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comparative evaluation of human pulp tissue dissolution by different concentrations of chlorine dioxide Calcium Hypochlorite and sodium Hypochlorite an in vitro study
Journal of Conservative Dentistry, 2014Co-Authors: Sonali Taneja, Neha Mishra, Shubhra MalikAbstract:Introduction: Irrigation plays an indispensable role in removal of tissue remnants and debris from the complicated root canal system. This study compared the human pulp tissue dissolution by different concentrations of chlorine dioxide, Calcium Hypochlorite and sodium Hypochlorite. Materials and Methods: Pulp tissue was standardized to a weight of 9 mg for each sample. In all,60 samples obtained were divided into 6 groups according to the irrigating solution used- 2.5% sodium Hypochlorite (NaOCl), 5.25% NaOCl, 5% Calcium Hypochlorite (Ca(OCl) 2 ), 10% Ca(OCl) 2 , 5%chlorine dioxide (ClO 2 ) and 13% ClO 2 . Pulp tissue was placed in each test tube carrying irrigants of measured volume (5ml) according to their specified subgroup time interval: 30 minutes (Subgroup A) and 60 minutes (Subgroup B). The solution from each sample test tube was filtered and was left for drying overnight. The residual weight was calculated by filtration method. Results: Mean tissue dissolution increases with increase in time period. Results showed 5.25% NaOCl to be most effective at both time intervals followed by 2.5% NaOCl at 60 minutes, 10%Ca(OCl) 2 and 13% ClO 2 at 60 minutes. Least amount of tissue dissolving ability was demonstrated by 5% Ca(OCl) 2 and 5% ClO 2 at 30 minutes. Distilled water showed no pulp tissue dissolution. Conclusion: Withinthe limitations of the study, NaOCl most efficiently dissolved the pulp tissue at both concentrations and at both time intervals. Mean tissue dissolution by Ca(OCl) 2 and ClO 2 gradually increased with time and with their increase in concentration.
Bertan Yilmaz - One of the best experts on this subject based on the ideXlab platform.
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Calcium Hypochlorite on mouse embryonic fibroblast cells (NIH3T3) in vitro cytotoxicity and genotoxicity: MTT and comet assay
Molecular Biology Reports, 2020Co-Authors: Şehnaz Yilmaz, Oguz Yoldas, Aysin Dumani, Gizem Guler, Seda Ilgaz, Eylül Akbal, Hale Oksuz, Ayla Celik, Bertan YilmazAbstract:Antimicrobial irrigation solutions are widely used under clinical settings. Their effect on dental tissue is a subject of recent research, which aims for a safer irrigant for clinical use. In this regard, here our goal was to evaluate the cytotoxicity and the genotoxicity of Calcium Hypochlorite (Ca(OCl)_2) solution, along with NaOCl, on Mouse embryonic fibroblast cells (NIH3T3). First, Cells were treated either with NaOCl or Ca(OCl)_2 in a time- and dose-dependent manner for cytotoxicity by 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, then cell viability was calculated according to cell proliferation plots. Secondly, genotoxicity was assessed by Comet assay. Data were statistically analyzed by Tukey’s test ( P
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Calcium Hypochlorite on mouse embryonic fibroblast cells nih3t3 in vitro cytotoxicity and genotoxicity mtt and comet assay
Molecular Biology Reports, 2020Co-Authors: Şehnaz Yilmaz, Oguz Yoldas, Aysin Dumani, Gizem Guler, Seda Ilgaz, Eylül Akbal, Hale Oksuz, Ayla Celik, Bertan YilmazAbstract:Antimicrobial irrigation solutions are widely used under clinical settings. Their effect on dental tissue is a subject of recent research, which aims for a safer irrigant for clinical use. In this regard, here our goal was to evaluate the cytotoxicity and the genotoxicity of Calcium Hypochlorite (Ca(OCl)2) solution, along with NaOCl, on Mouse embryonic fibroblast cells (NIH3T3). First, Cells were treated either with NaOCl or Ca(OCl)2 in a time- and dose-dependent manner for cytotoxicity by 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, then cell viability was calculated according to cell proliferation plots. Secondly, genotoxicity was assessed by Comet assay. Data were statistically analyzed by Tukey’s test (P < .05). NaOCl and Ca(OCl)2 had similar effects on cellular viability at 3 and 6 h treatments. Cell viability of Ca(OCl)2 at concentrations of 0.0125%, 0.025%, 0.05%, or 0.125% was significantly lower than that of NaOCl at 24 h treatment (P < .05).Comparing Ca(OCl)2 and NaOCl treatments at all time points and concentrations, the damaged cell number of Ca(OCl)2 was almost fourfold higher than that of NaOCl. In conclusion, both, NaOCl and Ca(OCl)2 solutions were cytotoxic and genotoxic to NIH3T3, however, Ca(OCl)2 had a significantly higher damaged cell percentage than NaOCl at all time points and concentrations investigated.
