The Experts below are selected from a list of 1521 Experts worldwide ranked by ideXlab platform
Dominique Mazier - One of the best experts on this subject based on the ideXlab platform.
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rapid emergence of echinocandin resistance during Candida Kefyr fungemia treatment with caspofungin
Antimicrobial Agents and Chemotherapy, 2013Co-Authors: A Fekkar, I Meyer, J Y Brossas, Eric Dannaoui, M Palous, M Uzunov, S Nguyen, V Leblond, Dominique MazierAbstract:Echinocandin drugs are widely used for the treatment of candidemia. Resistance is considered rare, and only a few cases of breakthrough candidiasis in patients receiving echinocandin have been reported worldwide. We report here for the first time a Candida Kefyr isolate that acquired echinocandin resistance very rapidly after the initiation of caspofungin treatment for candidemia. We characterized the FKS gene mutation responsible for the resistance via the comparison of isolates sampled before and during treatment.
László Majoros - One of the best experts on this subject based on the ideXlab platform.
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decreased killing activity of micafungin against Candida guilliermondii Candida lusitaniae and Candida Kefyr in the presence of human serum
Microbial Drug Resistance, 2017Co-Authors: Qasem Saleh, Aliz Bozó, Renátó Kovács, Gabor Kardos, Rudolf Gesztelyi, Tamas Kardos, László MajorosAbstract:Currently, echinocandins are first-line drugs for treatment of invasive candidiasis. However, data on how serum influences killing activity of echinocandins against uncommon Candida species are limited. Therefore, the killing activity of micafungin in RPMI-1640 and in 50% serum was compared against Candida guilliermondii, Candida lusitaniae, and Candida Kefyr. Minimum inhibitory concentration (MIC) ranges in RPMI-1640 were 0.5-1, 0.12-0.25, and 0.06-0.12 mg/L, respectively. In 50% serum, MICs increased 32- to 256-fold. In RPMI-1640 ≥ 0.25, ≥4, and 32 mg/L micafungin was fungicidal against all four C. Kefyr (≤4.04 hours), two of three C. lusitaniae (≤16.10 hours), and two of three C. guilliermondii (≤12.30 hours), respectively. In 50% serum, all three species grew at ≤4 mg/L. Micafungin at 16-32 mg/L was fungicidal against all C. Kefyr isolates (≤3.03 hours) and at 32 mg/L was fungistatic against one of three C. lusitaniae isolates. Two C. lusitaniae isolates and all three C. guilliermondii grew at all tested concentrations. Adding human serum to susceptibility test media drew attention to loss of fungicidal or fungistatic activity of micafungin in the presence of serum proteins, which is not predicted by MICs in case of C. Kefyr and C. lusitaniae in RPMI-1640. Our results strongly suggest that micafungin and probably other echinocandins should be used with caution against rare Candida species.
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In vitro antifungal susceptibility patterns of planktonic and sessile Candida Kefyr clinical isolates.
Medical Mycology, 2017Co-Authors: Fruzsina Nagy, Aliz Bozó, Zoltán Tóth, Lajos Daróczi, László Majoros, Renátó KovácsAbstract:The activity of fluconazole, amphotericin B, caspofungin and micafungin was determined using XTT-based fungal damage assays against planktonic cells, early and mature biofilms of Candida Kefyr. Median MICs of planktonic cells were 0.25 mg/l, 0.25 mg/l, 0.5 mg/l, and 0.06 mg/l for fluconazole, amphotericin B, caspofungin, and micafungin, respectively. Fluconazole showed at least 50% fungal damage at ≥4 mg/l (51.5% ± 6.63% to 78.38% ± 1.44%) and at ≥128 mg/l (57.88% ± 9.2% to 67.25% ± 9.59%), while amphotericin B produced an even higher anti-biofilm effect at ≥0.5 mg/l (64.63% ± 6.79% to 79.5% ± 5.9%) and at ≥0.12 mg/l (77.63% ± 8.43% to 92.75% ± 1.89%) against early and mature biofilms, respectively. In case of micafungin, 50% fungal damage was observed at ≥0.06 mg/l (66.88% ± 10.16% to 98.63% ± 1.24%) and ≥0.25 mg/l (74.13% ± 10.77% to 99.38% ± 0.38%) for early and mature biofilms, respectively. Caspofungin-exposed cells showed an unexpected susceptibility pattern, that is, planktonic cells showed significantly decreased susceptibility at concentrations ranging from 0.015 mg/l to 1 mg/l compared to biofilms (P < .05-.01). The damage in planktonic cells and biofilms was comparable at higher concentrations. For planktonic cells and biofilms, 50% fungal damage was observed first at 0.5 mg/l (59.75% ± 3.16%) and at 0.06 mg/l (70.25% ± 10.95%), respectively. This unexpected pattern was confirmed using scanning electron microscopy. The unusual susceptibility pattern observed at lower caspofungin concentrations may explain the poorer outcome of caspofungin-treated C. Kefyr infections documented in certain patient populations. As this phenomenon was markedly less apparent in case of micafungin, these data suggest that micafungin may be a more reliable option than caspofungin for the treatment of C. Kefyr infections.
Leidy Carolina Cardona - One of the best experts on this subject based on the ideXlab platform.
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DETERMINACIÓN DE LA EFICIENCIA DEL PROCESO DE FERMENTACIÓN REALIZADO POR LAS LEVADURAS Candida Kefyr y Saccharomyces cerevisiae EN LA PRODUCCIÓN DE XILITOL
Revista de Investigaciones Universidad del Quindío, 2017Co-Authors: Henry Reyes Pineda, Bibiana Rosero, Leidy Carolina CardonaAbstract:Este trabajo consistió en determinar la eficiencia fermentativa de las levaduras Candida Kefyr y Saccharomyces cerevisiae en hidrolizado de hojas de Eucalyptus camaldulensis Dehnh. (Myrtaceae) para la producción de xilitol. Para la caracterización fisicoquímica del hidrolizado del material vegetal se midieron: humedad, acidez, grados brix, pH, densidad, azúcares reductores, análisis termogravimétrico (TGA), infrarrojo. Los análisis: de humedad, Aw, y de grados brix, arrojaron valores de: 4.29, 0,45 y 4,03, respectivamente. El análisis termogravimétrico mostró que hasta los 200 °C E. camaldulensis es relativamente estable y que a temperaturas superiores los principios activos empiezan a degradarse. La hidrólisis del material vegetal se realizó por ultrasonido a 37 °C por 20 minutos. El proceso fermentativo se realizó a una temperatura de 35 +/-2 °C, 200 rpm por un período de 30 horas, utilizándose las levaduras Candida Kefyr y Saccharomyces cerevisiae, y realizando análisis de azúcares reductores por DNS. Para ambas levaduras el resultado del análisis de azúcares reductores mostró que entre mayor tiempo de fermentación menor concentración de xilosa en el hidrolizado, resultado que indica que las levaduras utilizaron xilosa para producir xilitol. Saccharomyces Cerevisiae presentó mejor eficiencia consumiendo 51,42 % de la xilosa, en contraste, Cándida Kefyr consumió 36,81 %, ambas en un período de 30 horas.
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DETERMINACIÓN DE LA EFICIENCIA DEL PROCESO DE FERMENTACIÓN REALIZADO POR LAS LEVADURAS Candida Kefyr y Saccharomyces cerevisiae EN LA PRODUCCIÓN DE XILITOL DETERMINATION OF THE EFFICIENCY OF THE FERMENTATION PROCESS BY YEAST Candida Kefyr AND SACCHAR
2014Co-Authors: Henry Reyes Pineda, Bibiana Rosero, Leidy Carolina CardonaAbstract:This study consisted of determining the efficiency of fermentative yeasts Saccharomyces Cerevisiae and Candida Kefyr hydrolyzate leaves of Eucalyptus Camaldulensis Dehnh. (Myrtaceae) for xylitol production. For the physicochemical characterization of hydrolyzed plant, material humidity, acidity, degrees brix, pH, density, reducing sugars, thermogravimetric analysis (TGA), and infrared were measured.. Analysis: the results of moisture, Aw, and brix degrees were: 4.29, 0.45 and 4.03, respectively. Thermogravimetric analysis showed that E. Camaldulensis is relatively stable at higher temperatures (up to 200 ° C ) and the active ingredients start to degrade. The hydrolysis of plant material was carried out by ultrasound at 37 ° C for 20 minutes. The fermentation process was performed at a temperature of 35 + / -2 ° C, 200 rpm for a 30 hour period, using the yeast Saccharomyces Cerevisiae and Candida Kefyr, and performing analysis of reducing sugars by the DNS. For both yeast analyses, the results showed that reducing sugars between lower longer fermentation xylose concentrations in the hydrolysate. This result indicates that the yeast was used xylose to produce xylitol. Saccharomyces Cerevisiae showed better efficiency consuming xylose 51.42 %. Incontrast, Candida Kefyr consumed 36.81 %, both over a period of 30 hours.
C. Douchet - One of the best experts on this subject based on the ideXlab platform.
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identification phenotypique de Candida Kefyr sur un milieu tournesol agar tag modifie par le x gal
Journal De Mycologie Medicale, 2007Co-Authors: C. Douchet, A. Ferrer, J. Chandenier, D RichardlenobleAbstract:Resume Candida Kefyr est une levure pathogene emergente. Le milieu tournesol–agar (milieu TAG) nous ayant anterieurement permis de distinguer Candida albicans de Candida dubliniensis , nous avons recherche si la culture de C. Kefyr sur le milieu TAG conferait a cette levure des caracteristiques specifiques permettant de l’identifier. Sur le milieu TAG, C. Kefyr presente deux types de colonies, des colonies filamenteuses caracteristiques et des colonies non filamenteuses tres voisines morphologiquement de celles de C. albicans . Dans ce dernier cas, l’apport d’un substrat chromogene (X-Gal) specifique de la β-galactosidase, presente chez C. Kefyr et absente chez C. albicans permet de distinguer par la couleur bleue ou blanche des colonies ces deux especes de Candida .
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Identification phénotypique de Candida Kefyr sur un milieu tournesol–agar (TAG) modifié par le X-Gal
Journal de Mycologie Médicale, 2007Co-Authors: C. Douchet, A. Ferrer, J. Chandenier, D. Richard-lenobleAbstract:Resume Candida Kefyr est une levure pathogene emergente. Le milieu tournesol–agar (milieu TAG) nous ayant anterieurement permis de distinguer Candida albicans de Candida dubliniensis , nous avons recherche si la culture de C. Kefyr sur le milieu TAG conferait a cette levure des caracteristiques specifiques permettant de l’identifier. Sur le milieu TAG, C. Kefyr presente deux types de colonies, des colonies filamenteuses caracteristiques et des colonies non filamenteuses tres voisines morphologiquement de celles de C. albicans . Dans ce dernier cas, l’apport d’un substrat chromogene (X-Gal) specifique de la β-galactosidase, presente chez C. Kefyr et absente chez C. albicans permet de distinguer par la couleur bleue ou blanche des colonies ces deux especes de Candida .
Aline Oliveira Da Conceição - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial activity of coconut oil-in-water emulsion on Staphylococcus epidermidis and Escherichia coli EPEC associated to Candida Kefyr
Heliyon, 2018Co-Authors: Susy Ferraz De Oliveira, Ivon Pinheiro Lôbo, Rosenira Serpa Da Cruz, João Luciano Andrioli, Camila Pacheco S.m. Da Mata, Glêydison Amarante Soares, Edvan Do Carmo Santos, Elizama Aguiar-oliveira, Marcelo Franco, Aline Oliveira Da ConceiçãoAbstract:Abstract Candida Kefyr has been considered both a food-spoiling agent and a type of yeast with fermentation properties. In this study, the authors have evaluated the antimicrobial activity of a coconut oil-in-water emulsion associated to the presence of C. Kefyr. Fresh coconut kernels were used to obtain the coconut oil-in-water emulsion, the sterile coconut oil-in-water emulsion by decantation, and the coconut oil by means of a heating process. Commercial virgin coconut oil was also used. Agar diffusion, minimal inhibitory concentration and minimal bactericidal concentration (MIC/MBC) techniques were employed to evaluate antimicrobial activity against E. coli and S. epidermidis. The C. Kefyr isolate was identified and confirmed. Coconut milk-derived fatty acids were characterized by acid index and thin layer chromatography. Scanning electronic microscopy was performed to evaluate the morphology of the microorganisms. Lipase activity of C. Kefyr isolate was also detected. Coconut oil-in-water emulsion associated to C. Kefyr was active against both bacteria. Thin layer chromatography confirmed the presence of triglycerides and free fatty acids. The acid index showed higher acidity potential for the coconut oil-in-water emulsion. The microscopic images showed antibacterial action through the formation of membrane holes' and demonstrated yeast shape. All the above show new potentials for C. Kefyr and coconut oil-in-water emulsion in food technology.
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Antimicrobial activity of coconut oil-in-water emulsion on Staphylococcus epidermidis and Escherichia coli EPEC associated to Candida Kefyr
Elsevier, 2018Co-Authors: Susy Ferraz De Oliveira, Ivon Pinheiro Lôbo, Rosenira Serpa Da Cruz, João Luciano Andrioli, Camila Pacheco S.m. Da Mata, Glêydison Amarante Soares, Edvan Do Carmo Santos, Elizama Aguiar-oliveira, Marcelo Franco, Aline Oliveira Da ConceiçãoAbstract:Candida Kefyr has been considered both a food-spoiling agent and a type of yeast with fermentation properties. In this study, the authors have evaluated the antimicrobial activity of a coconut oil-in-water emulsion associated to the presence of C. Kefyr. Fresh coconut kernels were used to obtain the coconut oil-in-water emulsion, the sterile coconut oil-in-water emulsion by decantation, and the coconut oil by means of a heating process. Commercial virgin coconut oil was also used. Agar diffusion, minimal inhibitory concentration and minimal bactericidal concentration (MIC/MBC) techniques were employed to evaluate antimicrobial activity against E. coli and S. epidermidis. The C. Kefyr isolate was identified and confirmed. Coconut milk-derived fatty acids were characterized by acid index and thin layer chromatography. Scanning electronic microscopy was performed to evaluate the morphology of the microorganisms. Lipase activity of C. Kefyr isolate was also detected. Coconut oil-in-water emulsion associated to C. Kefyr was active against both bacteria. Thin layer chromatography confirmed the presence of triglycerides and free fatty acids. The acid index showed higher acidity potential for the coconut oil-in-water emulsion. The microscopic images showed antibacterial action through the formation of membrane holes' and demonstrated yeast shape. All the above show new potentials for C. Kefyr and coconut oil-in-water emulsion in food technology. Keywords: Microbiology, Food technology, Food scienc
