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Samir Droby - One of the best experts on this subject based on the ideXlab platform.
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2016Co-Authors: Hila Yehuda, Martin Goldway, Samir Droby, Michael Wisniewski, Meirav Bar-shimonAbstract:The effect of under- and overexpressed CoEXGI-encoded exoglucanase secreted by Candida Oleophila on the biocontrol of Penicillium digitatu
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characterization of extracellular lytic enzymes produced by the yeast biocontrol agent Candida Oleophila
Current Genetics, 2004Co-Authors: Meirav Barshimon, Hila Yehuda, Martin Goldway, Michael Wisniewski, B Weiss, Avinoam Daus, Lea Cohen, Alexsandra Kobeshnikov, Samir DrobyAbstract:The yeast Candida Oleophila, the base of the commercial product Aspire, is recommended for the control of postharvest decay of citrus and pome fruit. Competition for nutrients and space is believed to be the major mode of action. Involvement of fungal cell wall-degrading enzymes is also suggested to play a role in the mechanism of action of yeast antagonists. The present study showed that the yeast C. Oleophila is capable of producing and secreting various cell wall-degrading enzymes, including exo-beta-1,3-glucanase, chitinase and protease. Exo-beta-1,3-glucanase and chitinase were produced and maximized in the early stages of growth, whereas protease reached a maximum level only after 6-8 days. Production of exo-beta-1,3-glucanase, chitinase and protease was stimulated by the presence of cell wall fragments of Penicillium digitatum in the growth medium, in addition to glucose. This study also provided evidence that C. Oleophila is capable of secreting exo-beta-1,3-glucanase into the wounded surface of grapefruit. The role of exo-beta-1,3-glucanase ( CoEXG1) in the biocontrol activity of C. Oleophila was tested using CoEXG1-knockouts and double- CoEXG1 over-producing transformants. In vitro bioassays showed that wild-type C. Oleophila and exo-beta-1,3-glucanase over-expressing transformants had similar inhibitory effects on spore germination and germ-tube elongation; and both were more inhibitory to the fungus than the knockout transformant. In experiments conducted on fruit to test the biocontrol activity against infection by P. digitatum, no significant difference in inhibition was observed between transformants and untransformed C. Oleophila cells at the high concentrations of cells used, whereas at a lower concentration of yeast cells the knockout transformants appeared to be less effective.
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induction of resistance to penicillium digitatum in grapefruit by the yeast biocontrol agent Candida Oleophila
Phytopathology, 2002Co-Authors: Samir Droby, Victor Vinokur, B Weiss, L Cohen, Avinoam Daus, Eliezer E Goldschmidt, Ron PoratAbstract:ABSTRACT The yeast Candida Oleophila, the base of the commercial product Aspire, is recommended for the control of postharvest decay in citrus and pome fruit. Its modes of action include nutrient competition, site exclusion, and direct mycoparasitism. In the present study, we showed that application of Candida Oleophila to surface wounds or to intact ‘Marsh Seedless’ grapefruit elicited systemic resistance against Penicillium digitatum, the main postharvest pathogen of citrus fruit. The induction of pathogen resistance in fruit was already pronounced 24 h after elicitation; it was distance, concentration, and time dependent and restricted to the peel tissue closely surrounding the yeast application site. The induction of pathogen resistance required viable yeast cells at concentrations of 108 to 109 cells ml-1. Nonviable autoclaved or boiled yeast cells or lower yeast concentrations were ineffective in enhancing fruit disease resistance. Application of Candida Oleophila cell suspensions to grapefruit peel...
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control of brown rot and blue mold of peach and nectarine by short hot water brushing and yeast antagonists
Postharvest Biology and Technology, 2002Co-Authors: Ozgur Akgun Karabulut, Avinoam Daus, Lea Cohen, Susan Lurie, Batia Wiess, Samir DrobyAbstract:Abstract We examined the possible use of a hot water brushing (HWB) treatment to disinfect nectarine and peach fruit. In vitro studies showed Monilinia fructicola to be more sensitive than Penicillium expansum to high temperature. In vivo studies of inoculation of peach and nectarine fruit with M. fructicola followed by HWB at 55 or 60 °C for 20 s gave 70 and 80% decay inhibition, respectively, compared with the control. The inhibition percentage of M. fructicola with HWB were similar, if HWB was applied shortly after inoculation or 24 h later. In contrast, the sensitivity of P. expansum spores inoculated into wounds increased when the fruit were treated with HWB 24 h after the inoculation, compared with treatment just after inoculation. The combination of HWB with the application of yeast antagonists was also investigated. We found that treating fruit with HWB at 60 °C for 20 s and then dipping them into a cell suspension (108 cells per ml) of Candida spp. 24 h after inoculation with P. expansum reduced decay development by 60% compared with the controls. Another yeast antagonist, Candida Oleophila was inferior in its ability to control Penicillium expansum and M. fructicola compared with other Candida spp. The combination of HWB and Candida spp. showed no significant additional effect against M. fructicola, compared with HWB alone. HWB alone, at 60 °C for 20 s, reduced brown rot development to very low rates. In storage experiments covering 30 days at 0 °C and 10 days of shelf life, HWB and Candida spp. applied separately were each able to reduce natural decay incidence to commercially acceptable levels (
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Yeast Yeast 2003; 20: 771–780. Published online in Wiley InterScience (www.interscience.wiley.com). DOI: 10.1002/yea.1006 Research Article
2002Co-Authors: Hila Yehuda, Martin Goldway, Samir Droby, Michael Wisniewski, Meirav Bar-shimonAbstract:The effect of under- and overexpressed CoEXG1-encoded exoglucanase secreted by Candida Oleophila on the biocontrol of Penicillium digitatu
Martin Goldway - One of the best experts on this subject based on the ideXlab platform.
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2016Co-Authors: Hila Yehuda, Martin Goldway, Samir Droby, Michael Wisniewski, Meirav Bar-shimonAbstract:The effect of under- and overexpressed CoEXGI-encoded exoglucanase secreted by Candida Oleophila on the biocontrol of Penicillium digitatu
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RESEARCH ARTICLE
2015Co-Authors: Meirav Bar-shimon, Martin Goldway, Alexsandra Kobeshnikov, Æ Hila Yehuda, Æ Lea Cohen, Batia Weiss, Æ Avinoam Daus, Æ Michael Wisniewski, Æ Samir DrobyAbstract:Abstract The yeast Candida Oleophila, the base of the commercial product Aspire, is recommended for the control of postharvest decay of citrus and pome fruit. Competition for nutrients and space is believed to be the major mode of action. Involvement of fungal cell wall-degrading enzymes is also suggested to play a role in the mechanism of action of yeast antagonists. The present study showed that the yeast C. Oleophila is capable of producing and secreting various cell wall-degrading enzymes, including exo-b-1,3-glucanase, chitinase and protease. Exo-b-1,3-glucanase and chitinase were pro-duced and maximized in the early stages of growth, whereas protease reached a maximum level only after 6–8 days. Production of exo-b-1,3-glucanase, chitinase and protease was stimulated by the presence of cell wall fragments of Penicillium digitatum in the growth med-ium, in addition to glucose. This study also provided evidence that C. Oleophila is capable of secreting exo-b-1,3-glucanase into the wounded surface of grapefruit. The role of exo-b-1,3-glucanase (CoEXG1) in the biocontrol activity of C. Oleophila was tested using CoEXG1-knockouts and double-CoEXG1 over-produc-ing transformants. In vitro bioassays showed that wild-type C. Oleophila and exo-b-1,3-glucanase over-expressing transformants had similar inhibitory effects on spore germination and germ-tube elongation; and both were more inhibitory to the fungus than the knockout transformant. In experiments conducted on fruit to test the biocontrol activity against infection by P. digitatum, no significant difference in inhibition was observed between transformants and untransformed C. Oleophila cells at the high concentrations of cells used, whereas at a lower concentration of yeast cells the knockout transformants appeared to be less effective
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characterization of extracellular lytic enzymes produced by the yeast biocontrol agent Candida Oleophila
Current Genetics, 2004Co-Authors: Meirav Barshimon, Hila Yehuda, Martin Goldway, Michael Wisniewski, B Weiss, Avinoam Daus, Lea Cohen, Alexsandra Kobeshnikov, Samir DrobyAbstract:The yeast Candida Oleophila, the base of the commercial product Aspire, is recommended for the control of postharvest decay of citrus and pome fruit. Competition for nutrients and space is believed to be the major mode of action. Involvement of fungal cell wall-degrading enzymes is also suggested to play a role in the mechanism of action of yeast antagonists. The present study showed that the yeast C. Oleophila is capable of producing and secreting various cell wall-degrading enzymes, including exo-beta-1,3-glucanase, chitinase and protease. Exo-beta-1,3-glucanase and chitinase were produced and maximized in the early stages of growth, whereas protease reached a maximum level only after 6-8 days. Production of exo-beta-1,3-glucanase, chitinase and protease was stimulated by the presence of cell wall fragments of Penicillium digitatum in the growth medium, in addition to glucose. This study also provided evidence that C. Oleophila is capable of secreting exo-beta-1,3-glucanase into the wounded surface of grapefruit. The role of exo-beta-1,3-glucanase ( CoEXG1) in the biocontrol activity of C. Oleophila was tested using CoEXG1-knockouts and double- CoEXG1 over-producing transformants. In vitro bioassays showed that wild-type C. Oleophila and exo-beta-1,3-glucanase over-expressing transformants had similar inhibitory effects on spore germination and germ-tube elongation; and both were more inhibitory to the fungus than the knockout transformant. In experiments conducted on fruit to test the biocontrol activity against infection by P. digitatum, no significant difference in inhibition was observed between transformants and untransformed C. Oleophila cells at the high concentrations of cells used, whereas at a lower concentration of yeast cells the knockout transformants appeared to be less effective.
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Yeast Yeast 2003; 20: 771–780. Published online in Wiley InterScience (www.interscience.wiley.com). DOI: 10.1002/yea.1006 Research Article
2002Co-Authors: Hila Yehuda, Martin Goldway, Samir Droby, Michael Wisniewski, Meirav Bar-shimonAbstract:The effect of under- and overexpressed CoEXG1-encoded exoglucanase secreted by Candida Oleophila on the biocontrol of Penicillium digitatu
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A transformation system for the biocontrol yeast, Candida Oleophila, based on hygromycin B resistance.
Current Genetics, 2001Co-Authors: Hila Yehuda, Samir Droby, Michael Wisniewski, Martin GoldwayAbstract:Lithium acetate transformation and electroporation were applied to the biocontrol yeast, Candida Oleophila. The hygromycin B resistance gene, flanked by the phosphoglycerate kinase promoter and terminator of Candida tropicalis, served as the genetic selection marker. The transformation efficiency of electroporation was almost 400 times more efficient than that of the lithium acetate method. While incorporation of DNA, flanked by a sequence endogenous to C. Oleophila, transpired apparently by homologous recombination, the integration of DNA (that did not contain C. Oleophila DNA) occurred at random. Whereas transformants were observed with a linear segment of the plasmid, none were detected with the undigested plasmid. This system provides both a tool for the molecular analysis of the biocontrol mechanism of C. Oleophila and a means of tagging C. Oleophila for field studies.
Ozgur Akgun Karabulut - One of the best experts on this subject based on the ideXlab platform.
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integrated control of postharvest diseases of peaches with a yeast antagonist hot water and modified atmosphere packaging
Crop Protection, 2004Co-Authors: Ozgur Akgun Karabulut, Necati BaykalAbstract:Abstract An integrated approach was studied for the control of postharvest diseases of peaches including application of a yeast antagonist ( Candida Oleophila ), hot water treatment at 55°C for 10 s, and storage in modified atmosphere at 0°C. We evaluated the efficacy of all three tactics, both as stand-alone treatments and in combination. Hot water and yeast antagonist as stand-alone treatments were not effective in controlling Botrytis cinerea and Penicillium expansum infections on wound-inoculated peaches. In contrast, modified atmosphere packaging alone significantly reduced lesion diameters caused by the infections of both pathogens after 45 days storage at 0°C and 5 days at 24°C. The biocontrol activity of yeast antagonist was improved when yeast treatment was combined with modified atmosphere packaging. The results also indicate that all of the treatments significantly reduced the decay incidence caused by natural infections after 45 days of storage at 0°C followed by 5 days at 24°C. The highest efficacy was achieved by the combination of all three tactics. None of the treatments caused surface damage to fruits or impaired quality.
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control of brown rot and blue mold of peach and nectarine by short hot water brushing and yeast antagonists
Postharvest Biology and Technology, 2002Co-Authors: Ozgur Akgun Karabulut, Avinoam Daus, Lea Cohen, Susan Lurie, Batia Wiess, Samir DrobyAbstract:Abstract We examined the possible use of a hot water brushing (HWB) treatment to disinfect nectarine and peach fruit. In vitro studies showed Monilinia fructicola to be more sensitive than Penicillium expansum to high temperature. In vivo studies of inoculation of peach and nectarine fruit with M. fructicola followed by HWB at 55 or 60 °C for 20 s gave 70 and 80% decay inhibition, respectively, compared with the control. The inhibition percentage of M. fructicola with HWB were similar, if HWB was applied shortly after inoculation or 24 h later. In contrast, the sensitivity of P. expansum spores inoculated into wounds increased when the fruit were treated with HWB 24 h after the inoculation, compared with treatment just after inoculation. The combination of HWB with the application of yeast antagonists was also investigated. We found that treating fruit with HWB at 60 °C for 20 s and then dipping them into a cell suspension (108 cells per ml) of Candida spp. 24 h after inoculation with P. expansum reduced decay development by 60% compared with the controls. Another yeast antagonist, Candida Oleophila was inferior in its ability to control Penicillium expansum and M. fructicola compared with other Candida spp. The combination of HWB and Candida spp. showed no significant additional effect against M. fructicola, compared with HWB alone. HWB alone, at 60 °C for 20 s, reduced brown rot development to very low rates. In storage experiments covering 30 days at 0 °C and 10 days of shelf life, HWB and Candida spp. applied separately were each able to reduce natural decay incidence to commercially acceptable levels (
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evaluation of the use of sodium bicarbonate potassium sorbate and yeast antagonists for decreasing postharvest decay of sweet cherries
Postharvest Biology and Technology, 2001Co-Authors: Ozgur Akgun Karabulut, Susan Lurie, Samir DrobyAbstract:Abstract The effects of sodium bicarbonate (SBC), potassium sorbate (K-sor) and two yeast biocontrol agents were tested for suppression of postharvest decay of sweet cherries. SBC at 2% reduced decay incidence after storage for 30 days at 0 °C and 4 days shelf life eightfold compared with untreated controls. K-sor, however, was less effective than SBC in reducing decay under all storage conditions tested. Between the two yeast antagonists, Candida sp. was superior in its ability to reduce decay than that of Candida Oleophila . C. Oleophila failed to provide any protection of the fruit kept under various storage conditions . Modified atmosphere (MA) packaging combined with cold storage markedly enhanced the control activity of SBC and Candida sp. treatments.
Hila Yehuda - One of the best experts on this subject based on the ideXlab platform.
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2016Co-Authors: Hila Yehuda, Martin Goldway, Samir Droby, Michael Wisniewski, Meirav Bar-shimonAbstract:The effect of under- and overexpressed CoEXGI-encoded exoglucanase secreted by Candida Oleophila on the biocontrol of Penicillium digitatu
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characterization of extracellular lytic enzymes produced by the yeast biocontrol agent Candida Oleophila
Current Genetics, 2004Co-Authors: Meirav Barshimon, Hila Yehuda, Martin Goldway, Michael Wisniewski, B Weiss, Avinoam Daus, Lea Cohen, Alexsandra Kobeshnikov, Samir DrobyAbstract:The yeast Candida Oleophila, the base of the commercial product Aspire, is recommended for the control of postharvest decay of citrus and pome fruit. Competition for nutrients and space is believed to be the major mode of action. Involvement of fungal cell wall-degrading enzymes is also suggested to play a role in the mechanism of action of yeast antagonists. The present study showed that the yeast C. Oleophila is capable of producing and secreting various cell wall-degrading enzymes, including exo-beta-1,3-glucanase, chitinase and protease. Exo-beta-1,3-glucanase and chitinase were produced and maximized in the early stages of growth, whereas protease reached a maximum level only after 6-8 days. Production of exo-beta-1,3-glucanase, chitinase and protease was stimulated by the presence of cell wall fragments of Penicillium digitatum in the growth medium, in addition to glucose. This study also provided evidence that C. Oleophila is capable of secreting exo-beta-1,3-glucanase into the wounded surface of grapefruit. The role of exo-beta-1,3-glucanase ( CoEXG1) in the biocontrol activity of C. Oleophila was tested using CoEXG1-knockouts and double- CoEXG1 over-producing transformants. In vitro bioassays showed that wild-type C. Oleophila and exo-beta-1,3-glucanase over-expressing transformants had similar inhibitory effects on spore germination and germ-tube elongation; and both were more inhibitory to the fungus than the knockout transformant. In experiments conducted on fruit to test the biocontrol activity against infection by P. digitatum, no significant difference in inhibition was observed between transformants and untransformed C. Oleophila cells at the high concentrations of cells used, whereas at a lower concentration of yeast cells the knockout transformants appeared to be less effective.
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Yeast Yeast 2003; 20: 771–780. Published online in Wiley InterScience (www.interscience.wiley.com). DOI: 10.1002/yea.1006 Research Article
2002Co-Authors: Hila Yehuda, Martin Goldway, Samir Droby, Michael Wisniewski, Meirav Bar-shimonAbstract:The effect of under- and overexpressed CoEXG1-encoded exoglucanase secreted by Candida Oleophila on the biocontrol of Penicillium digitatu
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A transformation system for the biocontrol yeast, Candida Oleophila, based on hygromycin B resistance.
Current Genetics, 2001Co-Authors: Hila Yehuda, Samir Droby, Michael Wisniewski, Martin GoldwayAbstract:Lithium acetate transformation and electroporation were applied to the biocontrol yeast, Candida Oleophila. The hygromycin B resistance gene, flanked by the phosphoglycerate kinase promoter and terminator of Candida tropicalis, served as the genetic selection marker. The transformation efficiency of electroporation was almost 400 times more efficient than that of the lithium acetate method. While incorporation of DNA, flanked by a sequence endogenous to C. Oleophila, transpired apparently by homologous recombination, the integration of DNA (that did not contain C. Oleophila DNA) occurred at random. Whereas transformants were observed with a linear segment of the plasmid, none were detected with the undigested plasmid. This system provides both a tool for the molecular analysis of the biocontrol mechanism of C. Oleophila and a means of tagging C. Oleophila for field studies.
Luiz Carlos Forti - One of the best experts on this subject based on the ideXlab platform.
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Yeasts isolated from a fungus-growing ant nest, including the description of Trichosporon chiarellii sp. nov., an anamorphic basidiomycetous yeast.
International Journal of Systematic and Evolutionary Microbiology, 2010Co-Authors: Fernando C. Pagnocca, Mara F. C. Legaspe, Andre Rodrigues, Carla C. C. Ruivo, Nilson S. Nagamoto, Maurício Bacci, Luiz Carlos FortiAbstract:Thirty-nine yeast strains were recovered from a field nest of a primitive and putative novel attine ant species in the genus Myrmicocrypta (Hymenoptera: Formicidae: tribe Attini). Yeasts isolated from the fungus garden and waste deposit included Candida dubliniensis, Candida Oleophila, Cryptococcus haglerorum and Hanseniaspora uvarum. In addition, one morphological type was isolated overwhelmingly. Sequencing data of partial large-subunit (LSU) rDNA and the internal transcribed spacer (ITS) region coupled with morphological and physiological characterization accommodated this morphotype in a separate taxonomic position in relation to the known species of Trichosporon (Basidiomycota: Trichosporonales). Here, we propose a novel yeast species named Trichosporon chiarellii sp. nov. based on the description of 34 isolates; the type strain is strain FCP 540806T (=CBS 11177T).
