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Jun Hang - One of the best experts on this subject based on the ideXlab platform.
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Canine Caliciviruses of four serotypes from military and research dogs recovered in 1963−1978 belong to two phylogenetic clades in the Vesivirus genus
Virology Journal, 2018Co-Authors: Leonard N. Binn, Erica A. Norby, Ruth H. Marchwicki, Richard G. Jarman, Paul B. Keiser, Jun HangAbstract:Background Vesiviruses (family Caliciviridae ) had been shown capable of invading a variety of host species, raising concern of their zoonotic potential. Since the 1980’s, several Canine Caliciviruses (CaCV) isolates have been reported and are phylogenetically related to the vesiviruses with features distinct from both Vesicular exanthema of swine virus (VESV) and Feline Calicivirus (FCV) species in phylogeny, serology and cell culture specificities. Etiological studies of Canine diseases in dogs used for military services and laboratory studies were conducted in 1963–1978 at the Walter Reed Army Institute of Research. Multiple known and unknown viral pathogens including Caliciviruses were recovered. Methods Four unidentified isolates were recovered in Walter Reed Canine Cells (WRCC) from respiratory, fecal and penile specimens. Physicochemical tests, electron microscopy, viral cultivation in human and animal cells, antibody neutralization assays, and recently the genome sequencing were used to characterize the isolates. Sera from these dogs and their cohorts were tested with the isolates to determine origin and prevalence of the infections. Results The viral isolates were small non-enveloped spherical RNA virions, 27 to 42 nm in diameter with cup-like structures, indicating they are Caliciviruses. They propagated in WRCC and MDCK cells, not in either other Canine cells or human and other animal cells. Each isolate is antigenically distinct and react with dog sera in respective cohorts. The genomes have nucleotide identities ranging from 70.3% to 90.7% and encode the non-structural polyprotein (1810 amino acids), major capsid protein (691 amino acids) and minor structural protein (134 amino acids). They belong to two different phylogenetic clades in Vesivirus genus with close relation with Canine Calicivirus (CaCV). Conclusions These CaCV isolates have restricted cell tropism, antigenic diversity and genetic variation. Further investigation will shed light on antigenic relation to other vesiviruses, and its pathogenicity for dogs and potential infectivity to other animals. Together with the previously reported CaCV strains provides significant evidence to support the formation of a new CaCV species in the Vesivirus genus.
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Canine Caliciviruses of four serotypes from military and research dogs recovered in 1963 1978 belong to two phylogenetic clades in the vesivirus genus
Virology Journal, 2018Co-Authors: Leonard N. Binn, Erica A. Norby, Ruth H. Marchwicki, Richard G. Jarman, Paul B. Keiser, Jun HangAbstract:Vesiviruses (family Caliciviridae) had been shown capable of invading a variety of host species, raising concern of their zoonotic potential. Since the 1980’s, several Canine Caliciviruses (CaCV) isolates have been reported and are phylogenetically related to the vesiviruses with features distinct from both Vesicular exanthema of swine virus (VESV) and Feline Calicivirus (FCV) species in phylogeny, serology and cell culture specificities. Etiological studies of Canine diseases in dogs used for military services and laboratory studies were conducted in 1963–1978 at the Walter Reed Army Institute of Research. Multiple known and unknown viral pathogens including Caliciviruses were recovered. Four unidentified isolates were recovered in Walter Reed Canine Cells (WRCC) from respiratory, fecal and penile specimens. Physicochemical tests, electron microscopy, viral cultivation in human and animal cells, antibody neutralization assays, and recently the genome sequencing were used to characterize the isolates. Sera from these dogs and their cohorts were tested with the isolates to determine origin and prevalence of the infections. The viral isolates were small non-enveloped spherical RNA virions, 27 to 42 nm in diameter with cup-like structures, indicating they are Caliciviruses. They propagated in WRCC and MDCK cells, not in either other Canine cells or human and other animal cells. Each isolate is antigenically distinct and react with dog sera in respective cohorts. The genomes have nucleotide identities ranging from 70.3% to 90.7% and encode the non-structural polyprotein (1810 amino acids), major capsid protein (691 amino acids) and minor structural protein (134 amino acids). They belong to two different phylogenetic clades in Vesivirus genus with close relation with Canine Calicivirus (CaCV). These CaCV isolates have restricted cell tropism, antigenic diversity and genetic variation. Further investigation will shed light on antigenic relation to other vesiviruses, and its pathogenicity for dogs and potential infectivity to other animals. Together with the previously reported CaCV strains provides significant evidence to support the formation of a new CaCV species in the Vesivirus genus.
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Canine Caliciviruses of four serotypes from military and research dogs recovered in 1963 1978 belong to two phylogenetic clades in the vesivirus genus
Virology Journal, 2018Co-Authors: Leonard N. Binn, Erica A. Norby, Ruth H. Marchwicki, Richard G. Jarman, Paul B. Keiser, Jun HangAbstract:Vesiviruses (family Caliciviridae) had been shown capable of invading a variety of host species, raising concern of their zoonotic potential. Since the 1980’s, several Canine Caliciviruses (CaCV) isolates have been reported and are phylogenetically related to the vesiviruses with features distinct from both Vesicular exanthema of swine virus (VESV) and Feline Calicivirus (FCV) species in phylogeny, serology and cell culture specificities. Etiological studies of Canine diseases in dogs used for military services and laboratory studies were conducted in 1963–1978 at the Walter Reed Army Institute of Research. Multiple known and unknown viral pathogens including Caliciviruses were recovered. Four unidentified isolates were recovered in Walter Reed Canine Cells (WRCC) from respiratory, fecal and penile specimens. Physicochemical tests, electron microscopy, viral cultivation in human and animal cells, antibody neutralization assays, and recently the genome sequencing were used to characterize the isolates. Sera from these dogs and their cohorts were tested with the isolates to determine origin and prevalence of the infections. The viral isolates were small non-enveloped spherical RNA virions, 27 to 42 nm in diameter with cup-like structures, indicating they are Caliciviruses. They propagated in WRCC and MDCK cells, not in either other Canine cells or human and other animal cells. Each isolate is antigenically distinct and react with dog sera in respective cohorts. The genomes have nucleotide identities ranging from 70.3% to 90.7% and encode the non-structural polyprotein (1810 amino acids), major capsid protein (691 amino acids) and minor structural protein (134 amino acids). They belong to two different phylogenetic clades in Vesivirus genus with close relation with Canine Calicivirus (CaCV). These CaCV isolates have restricted cell tropism, antigenic diversity and genetic variation. Further investigation will shed light on antigenic relation to other vesiviruses, and its pathogenicity for dogs and potential infectivity to other animals. Together with the previously reported CaCV strains provides significant evidence to support the formation of a new CaCV species in the Vesivirus genus.
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Additional file 2: of Canine Caliciviruses of four serotypes from military and research dogs recovered in 1963â1978 belong to two phylogenetic clades in the Vesivirus genus
2018Co-Authors: Leonard N. Binn, Ruth H. Marchwicki, Erica Norby, Richard Jarman, Paul Keiser, Jun HangAbstract:Figure S2. Alignment of Canine Calicivirus (CaCV) major capsid protein VP1 sequences. The MUSCLE program in software Geneious version 10.0.9 was used in multi-sequence alignment of capsid proteins of CaCV type I viruses A128T, L198Â T and 48, and type II viruses W191R, 2117, and 3â68. The amino acid residues differing between strains are shown in color. (DOC 559 kb
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Additional file 1: of Canine Caliciviruses of four serotypes from military and research dogs recovered in 1963â1978 belong to two phylogenetic clades in the Vesivirus genus
2018Co-Authors: Leonard N. Binn, Ruth H. Marchwicki, Erica Norby, Richard Jarman, Paul Keiser, Jun HangAbstract:Figure S1. Genome structure of Canine Calicivirus (CaCV). The CaCV genome is approximately 8.5Â kb in length, containing three open-reading frames (yellow bars). ORF1 encodes putative non-structural polyprotein precursor which may be cleaved into seven mature proteins (green bars), including the RNA-dependent RNA polymerase (RdRP). ORF2 and ORF3 encode major capsid protein (VP1) and small capsid protein (VP2) respectively. There are three nucleotides between the stop codon TGA (in red) of ORF1 and the start codon ATG (in blue) of ORF2. ORF2 and ORF3 overlap by four nucleotides which contains the start codon (ATG) of ORF3 and stop codon (TGA) of ORF2 respectively. (DOC 61 kb
Masami Mochizuki - One of the best experts on this subject based on the ideXlab platform.
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Complete Nucleotide Sequence, Genome Organization and Phylogenic Analysis of the Canine Calicivirus
Virus Genes, 2002Co-Authors: Yuichi Matsuura, Yukinobu Tohya, Kazuya Nakamura, Frank Roerink, Kozo Takase, Hiroomi Akashi, Masami Mochizuki, Masayuki Shimojima, Takaaki SugimuraAbstract:The complete genomic sequence of Canine Calicivirus (CaCV) isolated from feces of a dog with diarrhea was determined. The CaCV genome, a positive-sense single-stranded RNA, contained 8513 nucleotides excluding the poly(A) tail and was longer than that of any other Calicivirus strain with a completely known sequence. There were three open reading frames (ORF1, nt 12–5801; ORF2, nt 5805–7880; and ORF3, nt 7877–8278). ORF1 encoded a polyprotein (calculated M _r of 214,802) which had the conserved motifs of non-structural proteins of other Caliciviruses and picornaviruses. Regions containing characteristic motifs in the non-structural polyprotein of CaCV showed highest similarity with those of the species Feline Calicivirus and Vesicular exanthema of swine virus in the genus Vesivirus . Phylogenic analysis indicated that CaCV formed a distinct branch within the genus. Our results strongly suggested that CaCV is a new species in the genus Vesivirus .
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early interaction of Canine Calicivirus with cells is the major determinant for its cell tropism in vitro
Veterinary Microbiology, 2002Co-Authors: Yasuko Maeda, Yukinobu Tohya, Masami Mochizuki, Yuichi Matsuura, Takaaki SugimuraAbstract:Canine Calicivirus (CaCV) No. 48 strain isolated from a dog with fatal diarrhea is known to be able to replicate in MDCK and primary dog kidney cells. In this study, two new Canine cell lines, MCM-B2 and MCA-B1, were determined to be permissive for CaCV No. 48, whereas other cell lines, including one Canine cell line, A-72, were non-permissive. Flow cytometric analysis indicated that CaCV No. 48 binds efficiently to the permissive cells and to some degree also to Vero cells that are non-permissive for the virus, but does not bind to the other non-permissive cells tested. Both the permissive and non-permissive cells could be transfected with genomic RNA from CaCV No. 48, resulting in the appearance of CPE, production of capsid antigen and release of infectious progeny. These results suggested that the early interaction of the virus with cells, probably by binding to a virus receptor on the cell membrane, is the major determinant of CaCV No. 48 cell tropism in vitro.
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Molecular and Seroepidemiological Evidence of Canine Calicivirus Infections in Japan
Journal of clinical microbiology, 2002Co-Authors: Masami Mochizuki, Yukinobu Tohya, Frank Roerink, Michiru Hashimoto, Yuichi Matsuura, Nobuo SasakiAbstract:Calicivirus infection of dogs was epidemiologically investigated by using Canine Calicivirus (CaCV) strain 48 as a reference. Similar RNA polymerase gene sequences and neutralizing antibodies against CaCV were detected in 1.7% of clinical specimens and 57% of serum samples, respectively, suggesting a high prevalence of CaCV in dog populations.
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Identification of conformational neutralizing epitopes on the capsid protein of Canine Calicivirus.
Journal of General Virology, 2001Co-Authors: Yuichi Matsuura, Yukinobu Tohya, Kozo Takase, Masami Mochizuki, Takaaki SugimuraAbstract:Two neutralizing monoclonal antibodies (MAbs) against Canine Calicivirus (CaCV), which has a distinct antigenicity from feline Calicivirus (FCV), were obtained. Both MAbs recognized conformational epitopes on the capsid protein of CaCV and were used to identify these epitopes. Neutralization-resistant variants of CaCV were selected in the presence of individual MAbs in a cell culture. Cross-neutralization tests using the variants indicated that the MAbs recognized functionally independent epitopes on the capsid protein. Recombinantly expressed ORF2 products (capsid precursors) of the variants showed no reactivity to the MAbs used for the selection, suggesting that the resistance was induced by a failing in binding of the MAbs to the variant capsid proteins. Several nucleotide changes resulting in amino acid substitutions in the capsid protein were found by sequence analysis. Reactivities of the MAbs to the revertant ORF2 products produced from each variant ORF2 by site-directed mutagenesis identified a single amino acid substitution in each variant capsid protein responsible for the failure of MAb binding. The amino acid residues related to forming the conformational neutralizing epitopes were located in regions equivalent to the 5′ and 3′ hypervariable regions of the FCV capsid protein, where antigenic sites were demonstrated in previous studies. The recombinant ORF2 products expressed in bacteria failed to induce neutralizing antibody, suggesting that neutralizing antibodies were only generated when properly folded capsid protein was used as an antigen. In CaCV, the conformational epitopes may play a more important role in neutralization than do linear epitopes.
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genetic analysis of a Canine Calicivirus evidence for a new clade of animal Caliciviruses
Veterinary Microbiology, 1999Co-Authors: Frank Roerink, Yukinobu Tohya, Michiru Hashimoto, Masami MochizukiAbstract:This paper describes the relationship of a Canine Calicivirus, named No.48, to other human and animal Caliciviruses, based on phylogeny of the 3' half of its genome. It was found that No.48 constitutes a unique lineage, most closely related but distinct from feline and San Miguel sea lion Caliciviruses.
Yukinobu Tohya - One of the best experts on this subject based on the ideXlab platform.
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Complete Nucleotide Sequence, Genome Organization and Phylogenic Analysis of the Canine Calicivirus
Virus Genes, 2002Co-Authors: Yuichi Matsuura, Yukinobu Tohya, Kazuya Nakamura, Frank Roerink, Kozo Takase, Hiroomi Akashi, Masami Mochizuki, Masayuki Shimojima, Takaaki SugimuraAbstract:The complete genomic sequence of Canine Calicivirus (CaCV) isolated from feces of a dog with diarrhea was determined. The CaCV genome, a positive-sense single-stranded RNA, contained 8513 nucleotides excluding the poly(A) tail and was longer than that of any other Calicivirus strain with a completely known sequence. There were three open reading frames (ORF1, nt 12–5801; ORF2, nt 5805–7880; and ORF3, nt 7877–8278). ORF1 encoded a polyprotein (calculated M _r of 214,802) which had the conserved motifs of non-structural proteins of other Caliciviruses and picornaviruses. Regions containing characteristic motifs in the non-structural polyprotein of CaCV showed highest similarity with those of the species Feline Calicivirus and Vesicular exanthema of swine virus in the genus Vesivirus . Phylogenic analysis indicated that CaCV formed a distinct branch within the genus. Our results strongly suggested that CaCV is a new species in the genus Vesivirus .
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early interaction of Canine Calicivirus with cells is the major determinant for its cell tropism in vitro
Veterinary Microbiology, 2002Co-Authors: Yasuko Maeda, Yukinobu Tohya, Masami Mochizuki, Yuichi Matsuura, Takaaki SugimuraAbstract:Canine Calicivirus (CaCV) No. 48 strain isolated from a dog with fatal diarrhea is known to be able to replicate in MDCK and primary dog kidney cells. In this study, two new Canine cell lines, MCM-B2 and MCA-B1, were determined to be permissive for CaCV No. 48, whereas other cell lines, including one Canine cell line, A-72, were non-permissive. Flow cytometric analysis indicated that CaCV No. 48 binds efficiently to the permissive cells and to some degree also to Vero cells that are non-permissive for the virus, but does not bind to the other non-permissive cells tested. Both the permissive and non-permissive cells could be transfected with genomic RNA from CaCV No. 48, resulting in the appearance of CPE, production of capsid antigen and release of infectious progeny. These results suggested that the early interaction of the virus with cells, probably by binding to a virus receptor on the cell membrane, is the major determinant of CaCV No. 48 cell tropism in vitro.
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Molecular and Seroepidemiological Evidence of Canine Calicivirus Infections in Japan
Journal of clinical microbiology, 2002Co-Authors: Masami Mochizuki, Yukinobu Tohya, Frank Roerink, Michiru Hashimoto, Yuichi Matsuura, Nobuo SasakiAbstract:Calicivirus infection of dogs was epidemiologically investigated by using Canine Calicivirus (CaCV) strain 48 as a reference. Similar RNA polymerase gene sequences and neutralizing antibodies against CaCV were detected in 1.7% of clinical specimens and 57% of serum samples, respectively, suggesting a high prevalence of CaCV in dog populations.
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Identification of conformational neutralizing epitopes on the capsid protein of Canine Calicivirus.
Journal of General Virology, 2001Co-Authors: Yuichi Matsuura, Yukinobu Tohya, Kozo Takase, Masami Mochizuki, Takaaki SugimuraAbstract:Two neutralizing monoclonal antibodies (MAbs) against Canine Calicivirus (CaCV), which has a distinct antigenicity from feline Calicivirus (FCV), were obtained. Both MAbs recognized conformational epitopes on the capsid protein of CaCV and were used to identify these epitopes. Neutralization-resistant variants of CaCV were selected in the presence of individual MAbs in a cell culture. Cross-neutralization tests using the variants indicated that the MAbs recognized functionally independent epitopes on the capsid protein. Recombinantly expressed ORF2 products (capsid precursors) of the variants showed no reactivity to the MAbs used for the selection, suggesting that the resistance was induced by a failing in binding of the MAbs to the variant capsid proteins. Several nucleotide changes resulting in amino acid substitutions in the capsid protein were found by sequence analysis. Reactivities of the MAbs to the revertant ORF2 products produced from each variant ORF2 by site-directed mutagenesis identified a single amino acid substitution in each variant capsid protein responsible for the failure of MAb binding. The amino acid residues related to forming the conformational neutralizing epitopes were located in regions equivalent to the 5′ and 3′ hypervariable regions of the FCV capsid protein, where antigenic sites were demonstrated in previous studies. The recombinant ORF2 products expressed in bacteria failed to induce neutralizing antibody, suggesting that neutralizing antibodies were only generated when properly folded capsid protein was used as an antigen. In CaCV, the conformational epitopes may play a more important role in neutralization than do linear epitopes.
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Expression and processing of the Canine Calicivirus capsid precursor.
The Journal of general virology, 2000Co-Authors: Y Matsuura, Yukinobu Tohya, Frank Roerink, M Onuma, M Mochizuki, T SugimuraAbstract:The ORF2 product of Canine Calicivirus (CaCV) was identified and its processing in mammalian cells was analysed. Immunoblot analysis revealed the presence of the 75 kDa capsid precursor in addition to a 57 kDa capsid protein and a 22 kDa N-terminal polypeptide in CaCV-infected cells treated at an elevated temperature. When the CaCV ORF2 was expressed in a transient mammalian expression system, only the 75 kDa precursor was detected in immunoblot analysis, suggesting that no post-translational processing occurred in this system. However, the precursor was processed to a 57 kDa protein and a 22 kDa polypeptide by the proteinase of feline Calicivirus (FCV) when this was co-expressed with ORF2. Processing was blocked by site-directed mutagenesis of the putative cleavage site in the capsid precursor. The results indicate that the proteinase of FCV can cleave the capsid precursor of CaCV to produce the mature capsid protein and that CaCV may have a similar proteinase.
Leonard N. Binn - One of the best experts on this subject based on the ideXlab platform.
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Canine Caliciviruses of four serotypes from military and research dogs recovered in 1963−1978 belong to two phylogenetic clades in the Vesivirus genus
Virology Journal, 2018Co-Authors: Leonard N. Binn, Erica A. Norby, Ruth H. Marchwicki, Richard G. Jarman, Paul B. Keiser, Jun HangAbstract:Background Vesiviruses (family Caliciviridae ) had been shown capable of invading a variety of host species, raising concern of their zoonotic potential. Since the 1980’s, several Canine Caliciviruses (CaCV) isolates have been reported and are phylogenetically related to the vesiviruses with features distinct from both Vesicular exanthema of swine virus (VESV) and Feline Calicivirus (FCV) species in phylogeny, serology and cell culture specificities. Etiological studies of Canine diseases in dogs used for military services and laboratory studies were conducted in 1963–1978 at the Walter Reed Army Institute of Research. Multiple known and unknown viral pathogens including Caliciviruses were recovered. Methods Four unidentified isolates were recovered in Walter Reed Canine Cells (WRCC) from respiratory, fecal and penile specimens. Physicochemical tests, electron microscopy, viral cultivation in human and animal cells, antibody neutralization assays, and recently the genome sequencing were used to characterize the isolates. Sera from these dogs and their cohorts were tested with the isolates to determine origin and prevalence of the infections. Results The viral isolates were small non-enveloped spherical RNA virions, 27 to 42 nm in diameter with cup-like structures, indicating they are Caliciviruses. They propagated in WRCC and MDCK cells, not in either other Canine cells or human and other animal cells. Each isolate is antigenically distinct and react with dog sera in respective cohorts. The genomes have nucleotide identities ranging from 70.3% to 90.7% and encode the non-structural polyprotein (1810 amino acids), major capsid protein (691 amino acids) and minor structural protein (134 amino acids). They belong to two different phylogenetic clades in Vesivirus genus with close relation with Canine Calicivirus (CaCV). Conclusions These CaCV isolates have restricted cell tropism, antigenic diversity and genetic variation. Further investigation will shed light on antigenic relation to other vesiviruses, and its pathogenicity for dogs and potential infectivity to other animals. Together with the previously reported CaCV strains provides significant evidence to support the formation of a new CaCV species in the Vesivirus genus.
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Canine Caliciviruses of four serotypes from military and research dogs recovered in 1963 1978 belong to two phylogenetic clades in the vesivirus genus
Virology Journal, 2018Co-Authors: Leonard N. Binn, Erica A. Norby, Ruth H. Marchwicki, Richard G. Jarman, Paul B. Keiser, Jun HangAbstract:Vesiviruses (family Caliciviridae) had been shown capable of invading a variety of host species, raising concern of their zoonotic potential. Since the 1980’s, several Canine Caliciviruses (CaCV) isolates have been reported and are phylogenetically related to the vesiviruses with features distinct from both Vesicular exanthema of swine virus (VESV) and Feline Calicivirus (FCV) species in phylogeny, serology and cell culture specificities. Etiological studies of Canine diseases in dogs used for military services and laboratory studies were conducted in 1963–1978 at the Walter Reed Army Institute of Research. Multiple known and unknown viral pathogens including Caliciviruses were recovered. Four unidentified isolates were recovered in Walter Reed Canine Cells (WRCC) from respiratory, fecal and penile specimens. Physicochemical tests, electron microscopy, viral cultivation in human and animal cells, antibody neutralization assays, and recently the genome sequencing were used to characterize the isolates. Sera from these dogs and their cohorts were tested with the isolates to determine origin and prevalence of the infections. The viral isolates were small non-enveloped spherical RNA virions, 27 to 42 nm in diameter with cup-like structures, indicating they are Caliciviruses. They propagated in WRCC and MDCK cells, not in either other Canine cells or human and other animal cells. Each isolate is antigenically distinct and react with dog sera in respective cohorts. The genomes have nucleotide identities ranging from 70.3% to 90.7% and encode the non-structural polyprotein (1810 amino acids), major capsid protein (691 amino acids) and minor structural protein (134 amino acids). They belong to two different phylogenetic clades in Vesivirus genus with close relation with Canine Calicivirus (CaCV). These CaCV isolates have restricted cell tropism, antigenic diversity and genetic variation. Further investigation will shed light on antigenic relation to other vesiviruses, and its pathogenicity for dogs and potential infectivity to other animals. Together with the previously reported CaCV strains provides significant evidence to support the formation of a new CaCV species in the Vesivirus genus.
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Canine Caliciviruses of four serotypes from military and research dogs recovered in 1963 1978 belong to two phylogenetic clades in the vesivirus genus
Virology Journal, 2018Co-Authors: Leonard N. Binn, Erica A. Norby, Ruth H. Marchwicki, Richard G. Jarman, Paul B. Keiser, Jun HangAbstract:Vesiviruses (family Caliciviridae) had been shown capable of invading a variety of host species, raising concern of their zoonotic potential. Since the 1980’s, several Canine Caliciviruses (CaCV) isolates have been reported and are phylogenetically related to the vesiviruses with features distinct from both Vesicular exanthema of swine virus (VESV) and Feline Calicivirus (FCV) species in phylogeny, serology and cell culture specificities. Etiological studies of Canine diseases in dogs used for military services and laboratory studies were conducted in 1963–1978 at the Walter Reed Army Institute of Research. Multiple known and unknown viral pathogens including Caliciviruses were recovered. Four unidentified isolates were recovered in Walter Reed Canine Cells (WRCC) from respiratory, fecal and penile specimens. Physicochemical tests, electron microscopy, viral cultivation in human and animal cells, antibody neutralization assays, and recently the genome sequencing were used to characterize the isolates. Sera from these dogs and their cohorts were tested with the isolates to determine origin and prevalence of the infections. The viral isolates were small non-enveloped spherical RNA virions, 27 to 42 nm in diameter with cup-like structures, indicating they are Caliciviruses. They propagated in WRCC and MDCK cells, not in either other Canine cells or human and other animal cells. Each isolate is antigenically distinct and react with dog sera in respective cohorts. The genomes have nucleotide identities ranging from 70.3% to 90.7% and encode the non-structural polyprotein (1810 amino acids), major capsid protein (691 amino acids) and minor structural protein (134 amino acids). They belong to two different phylogenetic clades in Vesivirus genus with close relation with Canine Calicivirus (CaCV). These CaCV isolates have restricted cell tropism, antigenic diversity and genetic variation. Further investigation will shed light on antigenic relation to other vesiviruses, and its pathogenicity for dogs and potential infectivity to other animals. Together with the previously reported CaCV strains provides significant evidence to support the formation of a new CaCV species in the Vesivirus genus.
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Additional file 2: of Canine Caliciviruses of four serotypes from military and research dogs recovered in 1963â1978 belong to two phylogenetic clades in the Vesivirus genus
2018Co-Authors: Leonard N. Binn, Ruth H. Marchwicki, Erica Norby, Richard Jarman, Paul Keiser, Jun HangAbstract:Figure S2. Alignment of Canine Calicivirus (CaCV) major capsid protein VP1 sequences. The MUSCLE program in software Geneious version 10.0.9 was used in multi-sequence alignment of capsid proteins of CaCV type I viruses A128T, L198Â T and 48, and type II viruses W191R, 2117, and 3â68. The amino acid residues differing between strains are shown in color. (DOC 559 kb
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Additional file 1: of Canine Caliciviruses of four serotypes from military and research dogs recovered in 1963â1978 belong to two phylogenetic clades in the Vesivirus genus
2018Co-Authors: Leonard N. Binn, Ruth H. Marchwicki, Erica Norby, Richard Jarman, Paul Keiser, Jun HangAbstract:Figure S1. Genome structure of Canine Calicivirus (CaCV). The CaCV genome is approximately 8.5Â kb in length, containing three open-reading frames (yellow bars). ORF1 encodes putative non-structural polyprotein precursor which may be cleaved into seven mature proteins (green bars), including the RNA-dependent RNA polymerase (RdRP). ORF2 and ORF3 encode major capsid protein (VP1) and small capsid protein (VP2) respectively. There are three nucleotides between the stop codon TGA (in red) of ORF1 and the start codon ATG (in blue) of ORF2. ORF2 and ORF3 overlap by four nucleotides which contains the start codon (ATG) of ORF3 and stop codon (TGA) of ORF2 respectively. (DOC 61 kb
Frank Roerink - One of the best experts on this subject based on the ideXlab platform.
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Complete Nucleotide Sequence, Genome Organization and Phylogenic Analysis of the Canine Calicivirus
Virus Genes, 2002Co-Authors: Yuichi Matsuura, Yukinobu Tohya, Kazuya Nakamura, Frank Roerink, Kozo Takase, Hiroomi Akashi, Masami Mochizuki, Masayuki Shimojima, Takaaki SugimuraAbstract:The complete genomic sequence of Canine Calicivirus (CaCV) isolated from feces of a dog with diarrhea was determined. The CaCV genome, a positive-sense single-stranded RNA, contained 8513 nucleotides excluding the poly(A) tail and was longer than that of any other Calicivirus strain with a completely known sequence. There were three open reading frames (ORF1, nt 12–5801; ORF2, nt 5805–7880; and ORF3, nt 7877–8278). ORF1 encoded a polyprotein (calculated M _r of 214,802) which had the conserved motifs of non-structural proteins of other Caliciviruses and picornaviruses. Regions containing characteristic motifs in the non-structural polyprotein of CaCV showed highest similarity with those of the species Feline Calicivirus and Vesicular exanthema of swine virus in the genus Vesivirus . Phylogenic analysis indicated that CaCV formed a distinct branch within the genus. Our results strongly suggested that CaCV is a new species in the genus Vesivirus .
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Molecular and Seroepidemiological Evidence of Canine Calicivirus Infections in Japan
Journal of clinical microbiology, 2002Co-Authors: Masami Mochizuki, Yukinobu Tohya, Frank Roerink, Michiru Hashimoto, Yuichi Matsuura, Nobuo SasakiAbstract:Calicivirus infection of dogs was epidemiologically investigated by using Canine Calicivirus (CaCV) strain 48 as a reference. Similar RNA polymerase gene sequences and neutralizing antibodies against CaCV were detected in 1.7% of clinical specimens and 57% of serum samples, respectively, suggesting a high prevalence of CaCV in dog populations.
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Expression and processing of the Canine Calicivirus capsid precursor.
The Journal of general virology, 2000Co-Authors: Y Matsuura, Yukinobu Tohya, Frank Roerink, M Onuma, M Mochizuki, T SugimuraAbstract:The ORF2 product of Canine Calicivirus (CaCV) was identified and its processing in mammalian cells was analysed. Immunoblot analysis revealed the presence of the 75 kDa capsid precursor in addition to a 57 kDa capsid protein and a 22 kDa N-terminal polypeptide in CaCV-infected cells treated at an elevated temperature. When the CaCV ORF2 was expressed in a transient mammalian expression system, only the 75 kDa precursor was detected in immunoblot analysis, suggesting that no post-translational processing occurred in this system. However, the precursor was processed to a 57 kDa protein and a 22 kDa polypeptide by the proteinase of feline Calicivirus (FCV) when this was co-expressed with ORF2. Processing was blocked by site-directed mutagenesis of the putative cleavage site in the capsid precursor. The results indicate that the proteinase of FCV can cleave the capsid precursor of CaCV to produce the mature capsid protein and that CaCV may have a similar proteinase.
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genetic analysis of a Canine Calicivirus evidence for a new clade of animal Caliciviruses
Veterinary Microbiology, 1999Co-Authors: Frank Roerink, Yukinobu Tohya, Michiru Hashimoto, Masami MochizukiAbstract:This paper describes the relationship of a Canine Calicivirus, named No.48, to other human and animal Caliciviruses, based on phylogeny of the 3' half of its genome. It was found that No.48 constitutes a unique lineage, most closely related but distinct from feline and San Miguel sea lion Caliciviruses.
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Organization of the Canine Calicivirus genome from the RNA polymerase gene to the poly(A) tail.
Journal of General Virology, 1999Co-Authors: Frank Roerink, Yukinobu Tohya, Michiru Hashimoto, Masami MochizukiAbstract:In recent years a wealth of data has become available about the Caliciviruses that infect humans, as well as those which infect a range of animal species, notably cats, rabbits, pigs and marine animals. However, in the two decades since the earliest reports of Calicivirus infection in dogs, very little has become known about the epidemiology, pathogenicity and molecular biology of the Caliciviruses that may infect Canines. In 1990, a Canine Calicivirus (CaCV) was isolated from a 2-month-old diarrhoeic domestic dog in Japan. This virus, which can be grown in cultured cells of Canine origin, has the classic 'Star of David' morphology of Caliciviruses, and the one major structural protein was shown to be immunogenic in dogs. In this study, a 3.8 kb region of the genome of this CaCV isolate from the RNA polymerase gene to the 3' poly(A) tail was cloned and sequenced, and phylogenetic analysis was undertaken in order to establish the relationship of CaCV to other animal and human Caliciviruses. This CaCV isolate had a nucleotide sequence, genomic organization and phylogenetic position closest to, but clearly distinct from, both feline Calicivirus and San Miguel sea lion virus isolates. These findings suggest that CaCV represents a new clade of animal Caliciviruses, presumably as a member of the recently proposed new genus Vesivirus.
