The Experts below are selected from a list of 843 Experts worldwide ranked by ideXlab platform
Steven Sieber - One of the best experts on this subject based on the ideXlab platform.
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Waterhouse-Friderichsen syndrome secondary to Capnocytophaga canimorsus septicemia and demonstration of bacteremia by peripheral blood smear.
Archives of pathology & laboratory medicine, 2000Co-Authors: Imran Mirza, John Wolk, Lajos Toth, Peter Rostenberg, Ramon N. Kranwinkel, Steven SieberAbstract:Abstract Waterhouse-Friderichsen syndrome caused by Capnocytophaga canimorsus septicemia was fatal in a previously healthy 47-year-old woman. The patient died suddenly in less than 12 hours after p...
Guy R. Cornelis - One of the best experts on this subject based on the ideXlab platform.
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Draft Genome Sequences of Three Capnocytophaga canimorsus Strains Isolated from Healthy Canine Oral Cavities
Genome announcements, 2015Co-Authors: Pablo Manfredi, Francesco Renzi, Guy R. CornelisAbstract:ABSTRACT Here, we present the draft genome sequences of three strains of Capnocytophaga canimorsus, each isolated from a different dog9s mouth. Genome analysis provided evidence that these organisms may belong to a different nonpathogenic subtype of C. canimorsus.
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Draft Genome Sequences of Three Capnocytophaga canimorsus Strains Isolated from Septic Patients.
Genome announcements, 2015Co-Authors: Pablo Manfredi, Francesco Renzi, Guy R. CornelisAbstract:ABSTRACT Capnocytophaga canimorsus is a bacterium from the normal oral flora of dogs and cats that causes rare generalized infections in humans. In an attempt to determine whether infections could be caused by a subset of strains and to identify pathogenicity factors, we sequenced the genomes of three strains isolated from human infections.
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Correction: The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG
2015Co-Authors: Francesco Renzi, Pablo Manfredi, Manuela Mally, Suzette Moes, Paul Jenö, Guy R. CornelisAbstract:Correction: The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG
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Complete Genome Sequence of the Dog Commensal and Human Pathogen Capnocytophaga canimorsus Strain 5
Journal of bacteriology, 2011Co-Authors: Pablo Manfredi, Marco Pagni, Guy R. CornelisAbstract:Capnocytophaga canimorsus is a commensal Gram-negative bacterium, originally isolated from a dog's mouth, that causes septicemia in humans. C. canimorsus has the unusual ability to feed on host cells, including phagocytes. This capacity depends on surface-exposed glycan-foraging systems. Here we present the first complete genome sequence of a C. canimorsus strain (Cc5).
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Genetic Tools for Studying Capnocytophaga canimorsus
Applied and environmental microbiology, 2008Co-Authors: Manuela Mally, Guy R. CornelisAbstract:Capnocytophaga canimorsus, a commensal bacterium from canine oral flora, has been isolated throughout the world from severe human infections caused by dog bites. Due to the low level of evolutionary relationship to Proteobacteria, genetic methods suitable for the genus Capnocytophaga needed to be established. Here, we show that Tn4351, derived from Bacteroides fragilis, could be introduced by conjugation into C. canimorsus and conferred resistance to erythromycin. By mapping and sequencing a naturally occurring plasmid isolated from a clinical isolate of C. canimorsus, we identified a repA gene that allowed us to construct Escherichia coli-Capnocytophaga shuttle vectors. Most commonly used antibiotic markers were not functional in C. canimorsus, but cefoxitin (cfxA), tetracycline (tetQ), and erythromycin (ermF) resistances could be used as markers for plasmid maintenance in C. canimorsus and even in some other Capnocytophaga spp. Shuttle vectors were introduced into C. canimorsus either by conjugation using the origin of transfer (oriT) of RP4 or by electrotransformation. Taking advantage of the promoter of ermF, an expression vector was constructed. Finally, a method that allows site-directed mutagenesis is described. All these genetic tools pave the way, not only for molecular studies of the pathogenesis of C. canimorsus, but also for studies of other oral Capnocytophaga species.
Rikki M.a. Graham - One of the best experts on this subject based on the ideXlab platform.
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Rapid diagnosis of Capnocytophaga canimorsus septic shock in an immunocompetent individual using real-time Nanopore sequencing: a case report
BMC Infectious Diseases, 2019Co-Authors: Seweryn Bialasiewicz, Tania Duarte, Vichitra Sukumaran, Alexandra Stewart, Sally Appleton, Miranda E. Pitt, Arnold Bainomugisa, Son Hoang Nguyen, Amy V. Jennison, Rikki M.a. GrahamAbstract:Rapid diagnosis and appropriate treatment is imperative in bacterial sepsis due increasing risk of mortality with every hour without appropriate antibiotic therapy. Atypical infections with fastidious organisms may take more than 4 days to diagnose leading to calls for improved methods for rapidly diagnosing sepsis. Capnocytophaga canimorsus is a slow-growing, fastidious gram-negative bacillus which is a common commensal within the mouths of dogs, but rarely cause infections in humans. C. canimorsus sepsis risk factors include immunosuppression, alcoholism and elderly age. Here we report on the application of emerging nanopore sequencing methods to rapidly diagnose an atypical case of C. canimorsus septic shock. A 62 year-old female patient was admitted to an intensive care unit with septic shock and multi-organ failure six days after a reported dog bite. Blood cultures were unable to detect a pathogen after 3 days despite observed intracellular bacilli on blood smears. Real-time nanopore sequencing was subsequently employed on whole blood to detect Capnocytophaga canimorsus in 19 h. The patient was not immunocompromised and did not have any other known risk factors. Whole-genome sequencing of clinical sample and of the offending dog’s oral swabs showed near-identical C. canimorsus genomes. The patient responded to antibiotic treatment and was discharged from hospital 31 days after admission. Use of real-time nanopore sequencing reduced the time-to-diagnosis of Capnocytophaga canimorsus in this case from 6.25 days to 19 h. Capnocytophaga canimorsus should be considered in cases of suspected sepsis involving cat or dog contact, irrespective of the patient’s known risk factors.
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Rapid Diagnosis of Capnocytophaga canimorsus Septic Shock in an Immunocompetent Individual Using Real-Time Nanopore Sequencing
2018Co-Authors: Seweryn Bialasiewicz, Tania Duarte, Vichitra Sukumaran, Alexandra Stewart, Sally Appleton, Miranda E. Pitt, Arnold Bainomugisa, Son Hoang Nguyen, Amy V. Jennison, Rikki M.a. GrahamAbstract:We present a case of Capnocytophaga canimorsus septic shock after a dog bite in an immunocompetent individual, where real-time nanopore metagenomic sequencing characterized the microbial agent within 19 hours, with subsequent confirmation using droplet digital PCR. Oral swabs from the dog demonstrated a nearly-identical C. canimorsus isolate by sequencing.
Joseph Lorenzo Smith - One of the best experts on this subject based on the ideXlab platform.
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A rare case of Waterhouse-Friderichsen syndrome caused by Capnocytophaga canimorsus in an immunocompetent patient
Infection, 2015Co-Authors: Joseph David Cooper, Robert Patrick Dorion, Joseph Lorenzo SmithAbstract:A 53-year-old Caucasian male with hypertension and active tobacco abuse presented to a community hospital with a 2-day history of vague abdominal pain, myalgia and increased lethargy after being bitten on his right hand by the family dog while camping just 3 days prior to symptom onset. He expired within 90 min upon arrival to our intensive care unit. Pre-mortem blood cultures grew a fastidious Gram-negative aerobic rod that was identified as Capnocytophaga canimorsus . Autopsy findings showed multi-organ disseminated intravascular coagulopathy with microthrombi along with bilateral adrenal hemorrhage and necrosis of the adrenal glands consistent with Waterhouse-Friderichsen syndrome. This case contributes to the medical literature as a rare presentation of Capnocytophaga canimorsus infection in an otherwise immunocompetent patient and stresses the importance of a thorough history taking and physical examination by clinicians along with prompt administration of appropriate antibiotics.
Imran Mirza - One of the best experts on this subject based on the ideXlab platform.
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Waterhouse-Friderichsen syndrome secondary to Capnocytophaga canimorsus septicemia and demonstration of bacteremia by peripheral blood smear.
Archives of pathology & laboratory medicine, 2000Co-Authors: Imran Mirza, John Wolk, Lajos Toth, Peter Rostenberg, Ramon N. Kranwinkel, Steven SieberAbstract:Abstract Waterhouse-Friderichsen syndrome caused by Capnocytophaga canimorsus septicemia was fatal in a previously healthy 47-year-old woman. The patient died suddenly in less than 12 hours after p...
