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Guy R. Cornelis - One of the best experts on this subject based on the ideXlab platform.
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capsular serovars of virulent Capnocytophaga canimorsus are shared by the closely related species c canis and c cynodegmi
Emerging microbes & infections, 2018Co-Authors: Francesco Renzi, Estelle Hess, Melanie Dol, Dunia Koudad, Elodie Carlier, Maria Ohlen, Edward R B Moore, Guy R. CornelisAbstract:Capnocytophaga canimorsus is a dog oral commensal bacterium that causes rare but life-threatening generalized infections in humans who have been in contact with its animal hosts. Two other dog commensals, Capnocytophaga canis and Capnocytophaga cynodegmi, cause rare, mild local infections. To date, nine capsular serovars have been described in C. canimorsus. Here, we serotyped 112 strains of Capnocytophaga spp. isolated from human infections. The C. canimorsus strains (86 of 96, 89.6%) belonged to serovars A, B, or C with relative frequencies of approximately 30% for each serovar. The high prevalence of the A, B, and C serovars in strains isolated from humans, compared to the previously described low prevalence of these serovars among dog isolates (7.6%), confirms that these three serovars are more virulent to humans than other serovars and suggests that the low incidence of disease may be linked to the low prevalence of the A, B, and C serovars in dogs. We serotyped six strains of C. canis and ten strains of C. cynodegmi and, surprisingly, found one C. canis and three C. cynodegmi strains to be of capsular serovar B. This observation prompted us to test 34 dog-isolated C. canis and 16 dog-isolated C. cynodegmi strains. We found four C. canis strains belonging to serovar A and one belonging to serovar F. In contrast, no dog-isolated C. cynodegmi strain could be typed with the available antisera. This work demonstrates that virulence-associated capsular polysaccharides (A, B, and C) are not specific to the C. canimorsus species.
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improvement of identification of Capnocytophaga canimorsus by matrix assisted laser desorption ionization time of flight mass spectrometry using enriched database
Diagnostic Microbiology and Infectious Disease, 2016Co-Authors: Amandine Magnette, Francesco Renzi, Guy R. Cornelis, Tedin Huang, Pierre Bogaerts, Youri GlupczynskiAbstract:Abstract Capnocytophaga canimorsus and Capnocytophaga cynodegmi can be transmitted from dogs or cats and cause serious human infections. We aimed to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify these two Capnocytophaga species. Ninety-four C. canimorsus and 10 C. cynodegmi isolates identified by 16S rRNA gene sequencing were analyzed. Using the MALDI BioTyper database, correct identification was achieved for only 16 of 94 (17%) C. canimorsus and all 10 C. cynodegmi strains, according to the manufacturer's log score specifications. Following the establishment of a complementary homemade reference database by addition of 51 C. canimorsus and 8 C. cynodegmi mass spectra, MALDI-TOF MS provided reliable identification to the species level for 100% of the 45 blind-coded Capnocytophaga isolates tested. MALDI-TOF MS can accurately identify C. canimorsus and C. cynodegmi using an enriched database and thus constitutes a valuable diagnostic tool in the clinical laboratory.
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Draft Genome Sequences of Three Capnocytophaga canimorsus Strains Isolated from Septic Patients.
Genome announcements, 2015Co-Authors: Pablo Manfredi, Francesco Renzi, Guy R. CornelisAbstract:ABSTRACT Capnocytophaga canimorsus is a bacterium from the normal oral flora of dogs and cats that causes rare generalized infections in humans. In an attempt to determine whether infections could be caused by a subset of strains and to identify pathogenicity factors, we sequenced the genomes of three strains isolated from human infections.
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Correction: The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG
2015Co-Authors: Francesco Renzi, Pablo Manfredi, Manuela Mally, Suzette Moes, Paul Jenö, Guy R. CornelisAbstract:Correction: The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG
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new iron acquisition system in bacteroidetes
Infection and Immunity, 2015Co-Authors: Pablo Manfredi, Francesco Renzi, Guy R. Cornelis, Frederic Lauber, Katrin Hack, Estelle HessAbstract:Capnocytophaga canimorsus, a dog mouth commensal and a member of the Bacteroidetes phylum, causes rare but often fatal septicemia in humans that have been in contact with a dog. Here, we show that C. canimorsus strains isolated from human infections grow readily in heat-inactivated human serum and that this property depends on a typical polysaccharide utilization locus (PUL), namely, PUL3 in strain Cc5. PUL are a hallmark of Bacteroidetes, and they encode various products, including surface protein complexes that capture and process polysaccharides or glycoproteins. The archetype system is the Bacteroides thetaiotaomicron Sus system, devoted to starch utilization. Unexpectedly, PUL3 conferred the capacity to acquire iron from serotransferrin (STF), and this capacity required each of the seven encoded proteins, indicating that a whole Sus-like machinery is acting as an iron capture system (ICS), a new and unexpected function for Sus-like machinery. No siderophore could be detected in the culture supernatant of C. canimorsus, suggesting that the Sus-like machinery captures iron directly from transferrin, but this could not be formally demonstrated. The seven genes of the ICS were found in the genomes of several opportunistic pathogens from the Capnocytophaga and Prevotella genera, in different isolates of the severe poultry pathogen Riemerella anatipestifer, and in strains of Bacteroides fragilis and Odoribacter splanchnicus isolated from human infections. Thus, this study describes a new type of ICS that evolved in Bacteroidetes from a polysaccharide utilization system and most likely represents an important virulence factor in this group.
Francesco Renzi - One of the best experts on this subject based on the ideXlab platform.
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Septic shock caused by Capnocytophaga canis after a cat scratch
European Journal of Clinical Microbiology & Infectious Diseases, 2020Co-Authors: Viviane Donner, Francesco Renzi, Marta Buzzi, Vladimir Lazarevic, Nadia Gaïa, Myriam Girard, Gesuele Renzi, Abdessalam Cherkaoui, Jacques SchrenzelAbstract:Capnocytophaga canis is an uncommon cause of septic shock. Only three cases have been previously reported in the literature. In this article, we describe the case of a 70-year-old male admitted to the intensive care unit for septic shock of unknown origin. On day 2, one anaerobic bottle out of the two sets taken at admission turned positive with Gram-negative bacilli. The pathogen was identified by 16S rRNA gene as C . canis. The strain was characterized and compared with other clinical isolates of Capnocytophaga spp.
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capsular serovars of virulent Capnocytophaga canimorsus are shared by the closely related species c canis and c cynodegmi
Emerging microbes & infections, 2018Co-Authors: Francesco Renzi, Estelle Hess, Melanie Dol, Dunia Koudad, Elodie Carlier, Maria Ohlen, Edward R B Moore, Guy R. CornelisAbstract:Capnocytophaga canimorsus is a dog oral commensal bacterium that causes rare but life-threatening generalized infections in humans who have been in contact with its animal hosts. Two other dog commensals, Capnocytophaga canis and Capnocytophaga cynodegmi, cause rare, mild local infections. To date, nine capsular serovars have been described in C. canimorsus. Here, we serotyped 112 strains of Capnocytophaga spp. isolated from human infections. The C. canimorsus strains (86 of 96, 89.6%) belonged to serovars A, B, or C with relative frequencies of approximately 30% for each serovar. The high prevalence of the A, B, and C serovars in strains isolated from humans, compared to the previously described low prevalence of these serovars among dog isolates (7.6%), confirms that these three serovars are more virulent to humans than other serovars and suggests that the low incidence of disease may be linked to the low prevalence of the A, B, and C serovars in dogs. We serotyped six strains of C. canis and ten strains of C. cynodegmi and, surprisingly, found one C. canis and three C. cynodegmi strains to be of capsular serovar B. This observation prompted us to test 34 dog-isolated C. canis and 16 dog-isolated C. cynodegmi strains. We found four C. canis strains belonging to serovar A and one belonging to serovar F. In contrast, no dog-isolated C. cynodegmi strain could be typed with the available antisera. This work demonstrates that virulence-associated capsular polysaccharides (A, B, and C) are not specific to the C. canimorsus species.
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improvement of identification of Capnocytophaga canimorsus by matrix assisted laser desorption ionization time of flight mass spectrometry using enriched database
Diagnostic Microbiology and Infectious Disease, 2016Co-Authors: Amandine Magnette, Francesco Renzi, Guy R. Cornelis, Tedin Huang, Pierre Bogaerts, Youri GlupczynskiAbstract:Abstract Capnocytophaga canimorsus and Capnocytophaga cynodegmi can be transmitted from dogs or cats and cause serious human infections. We aimed to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify these two Capnocytophaga species. Ninety-four C. canimorsus and 10 C. cynodegmi isolates identified by 16S rRNA gene sequencing were analyzed. Using the MALDI BioTyper database, correct identification was achieved for only 16 of 94 (17%) C. canimorsus and all 10 C. cynodegmi strains, according to the manufacturer's log score specifications. Following the establishment of a complementary homemade reference database by addition of 51 C. canimorsus and 8 C. cynodegmi mass spectra, MALDI-TOF MS provided reliable identification to the species level for 100% of the 45 blind-coded Capnocytophaga isolates tested. MALDI-TOF MS can accurately identify C. canimorsus and C. cynodegmi using an enriched database and thus constitutes a valuable diagnostic tool in the clinical laboratory.
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Draft Genome Sequences of Three Capnocytophaga canimorsus Strains Isolated from Septic Patients.
Genome announcements, 2015Co-Authors: Pablo Manfredi, Francesco Renzi, Guy R. CornelisAbstract:ABSTRACT Capnocytophaga canimorsus is a bacterium from the normal oral flora of dogs and cats that causes rare generalized infections in humans. In an attempt to determine whether infections could be caused by a subset of strains and to identify pathogenicity factors, we sequenced the genomes of three strains isolated from human infections.
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Correction: The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG
2015Co-Authors: Francesco Renzi, Pablo Manfredi, Manuela Mally, Suzette Moes, Paul Jenö, Guy R. CornelisAbstract:Correction: The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG
Youri Glupczynski - One of the best experts on this subject based on the ideXlab platform.
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improvement of identification of Capnocytophaga canimorsus by matrix assisted laser desorption ionization time of flight mass spectrometry using enriched database
Diagnostic Microbiology and Infectious Disease, 2016Co-Authors: Amandine Magnette, Francesco Renzi, Guy R. Cornelis, Tedin Huang, Pierre Bogaerts, Youri GlupczynskiAbstract:Abstract Capnocytophaga canimorsus and Capnocytophaga cynodegmi can be transmitted from dogs or cats and cause serious human infections. We aimed to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify these two Capnocytophaga species. Ninety-four C. canimorsus and 10 C. cynodegmi isolates identified by 16S rRNA gene sequencing were analyzed. Using the MALDI BioTyper database, correct identification was achieved for only 16 of 94 (17%) C. canimorsus and all 10 C. cynodegmi strains, according to the manufacturer's log score specifications. Following the establishment of a complementary homemade reference database by addition of 51 C. canimorsus and 8 C. cynodegmi mass spectra, MALDI-TOF MS provided reliable identification to the species level for 100% of the 45 blind-coded Capnocytophaga isolates tested. MALDI-TOF MS can accurately identify C. canimorsus and C. cynodegmi using an enriched database and thus constitutes a valuable diagnostic tool in the clinical laboratory.
Pablo Manfredi - One of the best experts on this subject based on the ideXlab platform.
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Draft Genome Sequences of Three Capnocytophaga cynodegmi Strains Isolated from the Oral Cavity of Healthy Dogs
2016Co-Authors: Pablo Manfredi, Francesco A Renzi, Guy B R. CornelisaAbstract:Here, we present the draft genome sequences of three strains of Capnocytophaga cynodegmi. In contrast to the very close rela-tionship among them, C. cynodegmi and Capnocytophaga canimorsus differ dramatically in terms of virulence in humans. Com-parative genomics provided some understanding on how Capnocytophaga species may switch from being dog commensals to human pathogens
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New Iron Acquisition System in Bacteroidetes
2016Co-Authors: Pablo Manfredi, Frédéric A Lauber, Francesco B Renzi, Katrin B Hack, Estelle B Hess, Guy B R. CornelisaAbstract:Capnocytophaga canimorsus, a dog mouth commensal and a member of the Bacteroidetes phylum, causes rare but often fatal septicemia in humans that have been in contact with a dog. Here, we show that C. canimorsus strains isolated from human infec-tions grow readily in heat-inactivated human serum and that this property depends on a typical polysaccharide utilization locus (PUL), namely, PUL3 in strain Cc5. PUL are a hallmark of Bacteroidetes, and they encode various products, including surface protein complexes that capture and process polysaccharides or glycoproteins. The archetype system is the Bacteroides thetaiotaomicron Sus system, devoted to starch utilization. Unexpectedly, PUL3 conferred the capacity to acquire iron from se-rotransferrin (STF), and this capacity required each of the seven encoded proteins, indicating that a whole Sus-like machinery is acting as an iron capture system (ICS), a new and unexpected function for Sus-like machinery. No siderophore could be detected in the culture supernatant of C. canimorsus, suggesting that the Sus-like machinery captures iron directly from transferrin, but this could not be formally demonstrated. The seven genes of the ICS were found in the genomes of several opportunistic patho-gens from the Capnocytophaga and Prevotella genera, in different isolates of the severe poultry pathogen Riemerella anatipes-tifer, and in strains of Bacteroides fragilis andOdoribacter splanchnicus isolated from human infections. Thus, this study de-scribes a new type of ICS that evolved in Bacteroidetes from a polysaccharide utilization system andmost likely represents an important virulence factor in this group. Capnocytophaga canimorsus is a commensal bacterium from theoral cavity of dogs that is regularly isolated, since its descrip
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Draft Genome Sequences of Three Capnocytophaga canimorsus Strains Isolated from Septic Patients.
Genome announcements, 2015Co-Authors: Pablo Manfredi, Francesco Renzi, Guy R. CornelisAbstract:ABSTRACT Capnocytophaga canimorsus is a bacterium from the normal oral flora of dogs and cats that causes rare generalized infections in humans. In an attempt to determine whether infections could be caused by a subset of strains and to identify pathogenicity factors, we sequenced the genomes of three strains isolated from human infections.
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Correction: The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG
2015Co-Authors: Francesco Renzi, Pablo Manfredi, Manuela Mally, Suzette Moes, Paul Jenö, Guy R. CornelisAbstract:Correction: The N-glycan Glycoprotein Deglycosylation Complex (Gpd) from Capnocytophaga canimorsus Deglycosylates Human IgG
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new iron acquisition system in bacteroidetes
Infection and Immunity, 2015Co-Authors: Pablo Manfredi, Francesco Renzi, Guy R. Cornelis, Frederic Lauber, Katrin Hack, Estelle HessAbstract:Capnocytophaga canimorsus, a dog mouth commensal and a member of the Bacteroidetes phylum, causes rare but often fatal septicemia in humans that have been in contact with a dog. Here, we show that C. canimorsus strains isolated from human infections grow readily in heat-inactivated human serum and that this property depends on a typical polysaccharide utilization locus (PUL), namely, PUL3 in strain Cc5. PUL are a hallmark of Bacteroidetes, and they encode various products, including surface protein complexes that capture and process polysaccharides or glycoproteins. The archetype system is the Bacteroides thetaiotaomicron Sus system, devoted to starch utilization. Unexpectedly, PUL3 conferred the capacity to acquire iron from serotransferrin (STF), and this capacity required each of the seven encoded proteins, indicating that a whole Sus-like machinery is acting as an iron capture system (ICS), a new and unexpected function for Sus-like machinery. No siderophore could be detected in the culture supernatant of C. canimorsus, suggesting that the Sus-like machinery captures iron directly from transferrin, but this could not be formally demonstrated. The seven genes of the ICS were found in the genomes of several opportunistic pathogens from the Capnocytophaga and Prevotella genera, in different isolates of the severe poultry pathogen Riemerella anatipestifer, and in strains of Bacteroides fragilis and Odoribacter splanchnicus isolated from human infections. Thus, this study describes a new type of ICS that evolved in Bacteroidetes from a polysaccharide utilization system and most likely represents an important virulence factor in this group.
Amandine Magnette - One of the best experts on this subject based on the ideXlab platform.
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improvement of identification of Capnocytophaga canimorsus by matrix assisted laser desorption ionization time of flight mass spectrometry using enriched database
Diagnostic Microbiology and Infectious Disease, 2016Co-Authors: Amandine Magnette, Francesco Renzi, Guy R. Cornelis, Tedin Huang, Pierre Bogaerts, Youri GlupczynskiAbstract:Abstract Capnocytophaga canimorsus and Capnocytophaga cynodegmi can be transmitted from dogs or cats and cause serious human infections. We aimed to evaluate the ability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to identify these two Capnocytophaga species. Ninety-four C. canimorsus and 10 C. cynodegmi isolates identified by 16S rRNA gene sequencing were analyzed. Using the MALDI BioTyper database, correct identification was achieved for only 16 of 94 (17%) C. canimorsus and all 10 C. cynodegmi strains, according to the manufacturer's log score specifications. Following the establishment of a complementary homemade reference database by addition of 51 C. canimorsus and 8 C. cynodegmi mass spectra, MALDI-TOF MS provided reliable identification to the species level for 100% of the 45 blind-coded Capnocytophaga isolates tested. MALDI-TOF MS can accurately identify C. canimorsus and C. cynodegmi using an enriched database and thus constitutes a valuable diagnostic tool in the clinical laboratory.
