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Kexuan Tang - One of the best experts on this subject based on the ideXlab platform.
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Isolation and molecular characterization of a cax gene from Capsella Bursa-Pastoris
Biocell, 2008Co-Authors: Wen Zhang, Xinglong Wang, Kexuan TangAbstract:A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursa- pastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. Bursa-Pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na + /Ca 2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed ex- tensive homology with CAX from other plant species. The expression analysis by different treatments indi- cated that Cbcax51 could be activated by cold triggering and was related to the cold acclimation process, but its expression is regulated negatively by drought and not affected by ABA or salt.
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Molecular cloning and characterization of cold-responsive gene Cbrci35 from Capsella Bursa-Pastoris
Biologia, 2007Co-Authors: Wen Zhang, Xinglong Wang, Xuanwei Zhou, Kexuan TangAbstract:A new rare cold-inducible (RCI) gene designated Cbrci35 was cloned from Capsella Bursa-Pastoris, an edible wild herb, using the rapid amplification of cDNA ends (RACE) method. The full-length cDNA of Cbrci35 (Database Accession No.: AY566573) was 1300 bp and contained a 978 bp ORF encoding a precursor of 326 amino acid residues with a 23 amino acids signal peptide. The predicted Cbrci35 protein contained a peroxidase active site and proximal heme-ligand signatures, an RGD cell attachment sequence motif and two leucine zipper pattern motifs. Bioinformatics analysis revealed that Cbrci35 has a high level of similarity with RCI genes from Arabidopsis thaliana and peroxidases genes from other plants. RT-PCR analysis revealed that Cbrci35 expressed only in root. A cold acclimation assay showed that Cbrci35 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. But expression was not induced exposed to dehydration, salt stress or abscisic acid, indicating that it might be subjected specifically to cold regulation. These results indicate that Cbrci35 is an analogue of RCI genes and may participate in cold-response or increasing the freezing tolerance of plants.
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Molecular cloning and characterization of a novel ice gene from Capsella Bursa-Pastoris
Molecular Biology, 2005Co-Authors: Xinglong Wang, Kexuan TangAbstract:A new ice gene (designated as Cbice53, an inducer of CBF expression) was cloned from Capsella Bursa-Pastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbice53 was 1811 bp long, with a 1476-bp open reading frame (ORF) encoding a Myc-like protein of 492 amino acids. The predicted CbICE53 protein contained a potential basic helix-loop-helix domain, a nuclear localization signal (NLS), an RNA-binding region (RGG box), and N-glycosylation and kinase phosphorylation sites. Bioinformatic analysis showed that CbICE53 was highly homologous to ICE1 from Arabidopsis thaliana. Transcription of Cbice53 gene was induced transiently during salt and cold treatments, suggesting that it was somehow involved in cold acclimation. The results of our study indicate that the Cbice53 gene is a new member of the ice gene family and may have a role in cold and salt responsiveness in C. Bursa-Pastoris.
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Isolation and Molecular Characterization of a New CRT Binding Factor Gene from Capsella Bursa-Pastoris
Journal of Biochemistry and Molecular Biology, 2004Co-Authors: Xinglong Wang, Zhongxiang Deng, Yan Pi, Kexuan TangAbstract:A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella Bursa-Pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbf1, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbf16, and Bncbf17 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.
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Molecular Cloning and Characterization of a CBF Gene from Capsella Bursa-Pastoris
Dna Sequence, 2004Co-Authors: Xinglong Wang, Zhonghai Chen, Yongzhen Pang, Kexuan TangAbstract:A new CBF gene was cloned from Capsella Bursa-Pastoris(shepherd's purse) by rapid amplification of cDNA ends (RACE). The full-length cDNA of C. Bursa-Pastoris CBF gene (designated as Cbcbf) was 1034 bp long and contained a 657 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 219 amino acids. The predicted CbCBF protein was found to have a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and an acidic C-terminal half that might act as an activator domain. Bioinformatic analysis revealed that Cbcbf strongly resembled other CBF genes from Arabidopsis thaliana (cbf1, cbf2, cbf3) and Brassica napus (Bncbf5, Bncbf 7, Bncbf16 and Bncbf17). Subsequent cold acclimation assay showed that Cbcbf was relevant to cold acclimation. Our study implies that Cbcbf might have similar functions possessed by other CBF genes such as inducing the expression of some cold-regulated genes and increasing plants' freezing tolerance.
Barbara Neuffer - One of the best experts on this subject based on the ideXlab platform.
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Native range variation in Capsella Bursa-Pastoris (Brassicaceae) along a 2500 km latitudinal transect
Flora, 2011Co-Authors: Barbara NeufferAbstract:Abstract Capsella Bursa-Pastoris (L.) Medik. is a self compatible tetraploid annual species which has successfully spread to most continents and is mostly confined to man-made habitats. The colonising success may be associated with fine-scaled ecotypic differentiation, and regional adaptation. Seeds of 377 individuals from 40 provenances were collected over a distance of more than 2500 km from the Kola Peninsula in the north to the Caspian Sea in the south in Russia and from northern to southern Scandinavia. 3558 progenies were investigated in common garden field experiments in order to distinguish between phenotypic variability and genetic variation of quantitative life-history traits (onset of flowering, rosette diameter, leaf type, plant height, and branching and fruit characters). Genetic diversity and population structure was examined with isozyme analysis. Along both transects, Capsella showed clinal differentiation in phenotypic traits: e.g., the most northern and most southern provenances flowered earlier than the provenances from in between. A highly significant quadratic correlation exists between morphological traits and the degree of latitude. Distribution of leaf types showed no geographical pattern, unlike that observed for other regions. Allozyme variation follows a pattern of isolation by distance.
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Catching a ‘hopeful monster’: shepherd's purse (Capsella Bursa-Pastoris) as a model system to study the evolution of flower development
Journal of Experimental Botany, 2006Co-Authors: Maren Hintz, Conny Bartholmes, Pia Nutt, Janine M. Ziermann, Steffen Hameister, Barbara Neuffer, Günter TheissenAbstract:Capsella is a small genus within the mustard family (Brassicaceae). Its three species, however, show many evolutionary trends also observed in other Brassicaceae (including Arabidopsis) and far beyond, including transitions from a diploid, self-incompatible, obligatory outcrossing species with comparatively large and attractive flowers but a restricted distribution to a polyploid, self-compatible, predominantly selfing, invasive species with floral reductions. All these evolutionary transitions may have contributed to the fact that Capsella Bursa-Pastoris (shepherd's purse) has become one of the most widely distributed flowering plants on our planet. In addition, Capsella Bursa-Pastoris shows a phenomenon that, although rare, could be of great evolutionary importance, specifically the occurrence of a homeotic variety found in relatively stable populations in the wild. Several lines of evidence suggest that homeotic changes played a considerable role in floral evolution, but how floral homeotic varieties are established in natural populations has remained a highly controversial topic among evolutionary biologists. Due to its close relationship with the model plant Arabidopsis thaliana, numerous experimental tools are available for studying the genus Capsella, and further tools are currently being developed. Hence, Capsella provides great opportunities to investigate the evolution of flower development from molecular developmental genetics to field ecology and biogeography, and from morphological refinements to major structural transitions.
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Evolutionary Processes Associated with Biological Invasions in the Brassicaceae
Biological Invasions, 2003Co-Authors: Herbert Hurka, Walter Bleeker, Barbara NeufferAbstract:This review highlights evolutionary processes in the Brassicaceae which cause, accompany or are a consequence of biological invasions. Case studies in Capsella and Diplotaxis address the genetics of colonising species. The greatest colonising ability in Capsella Bursa-Pastoris is associated with polyploidy, predominant selfing, and high genetic diversity. Success of colonial populations seems to be due to the introduction of preadapted genotypes. Colonising species in Diplotaxis reveal contrasting evolutionary patterns. Genetic attributes in D. muralis include annuality, polyploidy, and predominant selfing. Very different from Capsella , D. muralis is nearly devoid of genetic diversity as revealed by molecular markers. In contrast to D. muralis , the colonising D. tenuifolia is perennial, diploid, self-incompatible, and displays high genetic diversity. Hybrid speciation, establishment of the hybrids in man-made habitats, stabilisation of their reproductive system, and reproductive isolation from the parent populations were analysed in Cardamine . The Nasturtium example highlights the importance of hybridisation for the evolution of invasiveness. The last case study concentrates on the evolutionary consequences of hybridisation between native and invading Rorippa species. Introgressive hybridisation between the invasive Rorippa austriaca and native Rorippa species is common and widespread in central Europe, and interspecific gene transfer has led to the formation of a new invasive genotype. Each successful invasion presents new aspects and sound case studies are needed in order to understand the ecology and evolution of the colonisation process and to enable us to assess the evolutionary consequences of biological invasions.
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RAPD ANALYSES IN COLONIAL AND ANCESTRAL POPULATIONS OF Capsella Bursa-Pastoris (L.) MED. (BRASSICACEAE)
Biochemical Systematics and Ecology, 1996Co-Authors: Barbara NeufferAbstract:Abstract Capsella Bursa-Pastoris is a neophyte in the New World. It arrived in California not before 200 years ago. Thirteen populations from California, seven from Spain and one from Germany were analyzed with RAPDs in order to trace the introduction history and to study the structure of the colonial populations. The results not only supported earlier hypotheses on the colonization of Capsella which were based in isozyme studies (Hurka, 1993; Hurka et al. , 1989) and phenotypic traits (Hurka and Neuffer, 1991), but helped also to understand the colonization history in more detail. Overall similarity in RAPD markers was greater between Californian Central Valley and Spanish populations than it was between Central Valley and Californian mountain populations. It is concluded that genotypes preadapted to Mediterranean climate conditions were brought to California by Spaniards from Mexico. Their ancestral populations might be found in Spain which is strongly argued for by RAPD markers. They now occupy the Central Valley of California. The Californian mountains are colonized by other genotypes, the source of which is not yet clear, but unlikely to be of Mediterranean climate origin. In general, colonial Californian populations are genetically less variable than the European populations, but more variable than one would conclude by isozyme studies. Population structures of the colonial populations as revealed by RAPD studies are in full accordance with the expected organization of genetic variability within and between populations of a predominantly self colonizing plant.
Stephen I. Wright - One of the best experts on this subject based on the ideXlab platform.
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Transposable element evolution in the allotetraploid Capsella Bursa-Pastoris
American Journal of Botany, 2016Co-Authors: J. Arvid Ågren, Hui-run Huang, Stephen I. WrightAbstract:Shifts in ploidy affect the evolutionary dynamics of genomes in a myriad of ways. Population genetic theory predicts that transposable element (TE) proliferation may follow because the genomewide efficacy of selection should be reduced and the increase in gene copies may mask the deleterious effects of TE insertions. Moreover, in allopolyploids, TEs may further accumulate because of hybrid breakdown of TE silencing. However, to date the evidence of TE proliferation following an increase in ploidy is mixed, and the relative importance of relaxed selection vs. silencing breakdown remains unclear. We used high-coverage whole-genome sequence data to evaluate the abundance, genomic distribution, and population frequencies of TEs in the self-fertilizing recent allotetraploid Capsella Bursa-Pastoris (Brassicaceae). We then compared the C. Bursa-Pastoris TE profile with that of its two parental diploid species, outcrossing C. grandiflora and self-fertilizing C. orientalis. We found no evidence that C. Bursa-Pastoris has experienced a large genomewide proliferation of TEs relative to its parental species. However, when centromeric regions are excluded, we found evidence of significantly higher abundance of retrotransposons in C. Bursa-Pastoris along the gene-rich chromosome arms compared with C. grandiflora and C. orientalis. The lack of a genomewide effect of allopolyploidy on TE abundance, combined with the increases TE abundance in gene-rich regions, suggests that relaxed selection rather than hybrid breakdown of host silencing explains the TE accumulation in C. Bursa-Pastoris. © 2016 Botanical Society of America.
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Transposable Element Evolution in the Allotetraploid Capsella Bursa-Pastoris
bioRxiv, 2016Co-Authors: J. Arvid Ågren, Hui-run Huang, Stephen I. WrightAbstract:Premise of the study Shifts in ploidy level will affect the evolutionary dynamics of genomes in a myriad of ways. Population genetic theory predicts that transposable element (TE) proliferation may follow because the genome wide efficacy of selection should be reduced and the increase in gene copies may mask the deleterious effects of TE insertions. However, to date the evidence of TE proliferation following an increase in ploidy is mixed, with some studies reporting results consistent with this scenario and others signs of genome downsizing. Methods We used high-coverage whole genome sequence data to evaluate the abundance, genomic distribution, and population frequencies of TEs in the self-fertilizing recent allotetraploid Capsella Bursa-Pastoris, a species with prior evidence for genome-wide reductions in selection at the amino acid level since the transition to selfing. We then compared the C. Bursa-Pastoris TE profile with that of its two parental species, outcrossing C. grandiflora and self-fertilzing C. orientalis. Key results We found no evidence that C. Bursa-Pastoris has experienced a large proliferation of TEs. Instead, the abundance, both overall and near genes, as well as the population frequencies of TEs, are intermediate to that of its two parental species C. grandiflora and C. orientalis. Conclusions The lack of shift in TE profile beyond additivity expectations in C. Bursa-Pastoris can be because of variety of factors. In general, we argue that allopolyploid lineages that retain high outcrossing should provide a "perfect storm" for TE proliferation, while highly selfing polyploids may generally experience TE loss.
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R scripts: MCMCglmm analyses of Capsella Bursa-Pastoris
2016Co-Authors: Dmytro Kryvokhyzha, Karl Holm, Jun Chen, Amandine Cornille, Sylvain Glémin, Stephen I. Wright, Ulf Lagercrantz, Martin LascouxAbstract:These scripts were used to perform correction for population structure in the analyses of gene expression and phenotypic variation in Capsella Bursa-Pastoris.
Xinglong Wang - One of the best experts on this subject based on the ideXlab platform.
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Isolation and molecular characterization of a cax gene from Capsella Bursa-Pastoris
Biocell, 2008Co-Authors: Wen Zhang, Xinglong Wang, Kexuan TangAbstract:A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursa- pastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. Bursa-Pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na + /Ca 2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed ex- tensive homology with CAX from other plant species. The expression analysis by different treatments indi- cated that Cbcax51 could be activated by cold triggering and was related to the cold acclimation process, but its expression is regulated negatively by drought and not affected by ABA or salt.
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Molecular cloning and characterization of cold-responsive gene Cbrci35 from Capsella Bursa-Pastoris
Biologia, 2007Co-Authors: Wen Zhang, Xinglong Wang, Xuanwei Zhou, Kexuan TangAbstract:A new rare cold-inducible (RCI) gene designated Cbrci35 was cloned from Capsella Bursa-Pastoris, an edible wild herb, using the rapid amplification of cDNA ends (RACE) method. The full-length cDNA of Cbrci35 (Database Accession No.: AY566573) was 1300 bp and contained a 978 bp ORF encoding a precursor of 326 amino acid residues with a 23 amino acids signal peptide. The predicted Cbrci35 protein contained a peroxidase active site and proximal heme-ligand signatures, an RGD cell attachment sequence motif and two leucine zipper pattern motifs. Bioinformatics analysis revealed that Cbrci35 has a high level of similarity with RCI genes from Arabidopsis thaliana and peroxidases genes from other plants. RT-PCR analysis revealed that Cbrci35 expressed only in root. A cold acclimation assay showed that Cbrci35 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. But expression was not induced exposed to dehydration, salt stress or abscisic acid, indicating that it might be subjected specifically to cold regulation. These results indicate that Cbrci35 is an analogue of RCI genes and may participate in cold-response or increasing the freezing tolerance of plants.
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Molecular cloning and characterization of a novel ice gene from Capsella Bursa-Pastoris
Molecular Biology, 2005Co-Authors: Xinglong Wang, Kexuan TangAbstract:A new ice gene (designated as Cbice53, an inducer of CBF expression) was cloned from Capsella Bursa-Pastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbice53 was 1811 bp long, with a 1476-bp open reading frame (ORF) encoding a Myc-like protein of 492 amino acids. The predicted CbICE53 protein contained a potential basic helix-loop-helix domain, a nuclear localization signal (NLS), an RNA-binding region (RGG box), and N-glycosylation and kinase phosphorylation sites. Bioinformatic analysis showed that CbICE53 was highly homologous to ICE1 from Arabidopsis thaliana. Transcription of Cbice53 gene was induced transiently during salt and cold treatments, suggesting that it was somehow involved in cold acclimation. The results of our study indicate that the Cbice53 gene is a new member of the ice gene family and may have a role in cold and salt responsiveness in C. Bursa-Pastoris.
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Isolation and Molecular Characterization of a New CRT Binding Factor Gene from Capsella Bursa-Pastoris
Journal of Biochemistry and Molecular Biology, 2004Co-Authors: Xinglong Wang, Zhongxiang Deng, Yan Pi, Kexuan TangAbstract:A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella Bursa-Pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbf1, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbf16, and Bncbf17 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.
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Molecular Cloning and Characterization of a CBF Gene from Capsella Bursa-Pastoris
Dna Sequence, 2004Co-Authors: Xinglong Wang, Zhonghai Chen, Yongzhen Pang, Kexuan TangAbstract:A new CBF gene was cloned from Capsella Bursa-Pastoris(shepherd's purse) by rapid amplification of cDNA ends (RACE). The full-length cDNA of C. Bursa-Pastoris CBF gene (designated as Cbcbf) was 1034 bp long and contained a 657 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 219 amino acids. The predicted CbCBF protein was found to have a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and an acidic C-terminal half that might act as an activator domain. Bioinformatic analysis revealed that Cbcbf strongly resembled other CBF genes from Arabidopsis thaliana (cbf1, cbf2, cbf3) and Brassica napus (Bncbf5, Bncbf 7, Bncbf16 and Bncbf17). Subsequent cold acclimation assay showed that Cbcbf was relevant to cold acclimation. Our study implies that Cbcbf might have similar functions possessed by other CBF genes such as inducing the expression of some cold-regulated genes and increasing plants' freezing tolerance.
Jianbo Wang - One of the best experts on this subject based on the ideXlab platform.
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Comparative analysis of the variable 3′ UTR and gene expression of the KIN and KIN-homologous LEA genes in Capsella Bursa-Pastoris
Plant Cell Reports, 2012Co-Authors: Peng Tao, Lifang Peng, Xiaojun Huang, Jianbo WangAbstract:As the crucial members of the cold-regulated ( COR ) gene family, KIN genes are involved in diverse abiotic stress responses in plants. In the present study, KIN genes from the widespread plant Capsella bursa - pastoris were identified and analyzed to better understand the powerful adaptation of this species. Two KIN genes were cloned and sequenced by 3′ RACE. As some COR genes are homologous to LEA genes, three KIN -homologous LEA genes were also identified. We deduced the amino acid sequences of the five proteins to estimate their phylogenetic relationships, and grouped them into three subfamilies (CI, CII, and CIII). Variable 3′ UTRs were found in CI, CII, and CIII genes. Using qPCR, we evaluated the transcriptional levels of the five genes in different organs and embryonic stages. Two CI genes were exclusively expressed in early embryos and flowers. The CII and CIII genes showed obvious up-regulation in young leaves after heat stress, cold stress, and ABA treatment. Two of the CI genes, however, rarely responded to those stresses in young leaves. In contrast, all five genes showed differential responses in flowers when C. bursa - pastoris plants were sprayed with ABA. Furthermore, the expression of these genes in C. bursa - pastoris was compared to that of the corresponding Arabidopsis genes, and similar gene expression profiles were found in both species. Our findings suggest that these five genes play different roles in development and the responses to abiotic stresses in C. bursa - pastoris . Key message We characterized two KIN and three KIN -homologous LEA genes, and analyzed their variable 3’UTR and organ-specific, embryo-developmental, stress-induced gene expression in Capsella Bursa-Pastoris .
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Characterization of eight cytosolic sHSP genes and their expression in Capsella Bursa-Pastoris
Biologia Plantarum, 2012Co-Authors: Jianbo WangAbstract:Small heat shock proteins (sHSPs) are crucial components of the plant response to heat shock. We identified and analyzed eight sHSP genes of Capsella Bursa-Pastoris to better understand the ability of this species to adapt. Eight genes were initially cloned and sequenced from the mature embryo cDNA pool. They belong to the cytosolic I (CI), cytosolic II (CII), and cytosolic III (CIII) subfamilies. One CI sHSP gene was homologous to that of C. rubella. Sequence analysis using 3′ RACE revealed that there are two or more variable 3′-untranslated regions (UTRs) in these sHSP transcripts. The transcriptional levels of the eight sHSP genes were analyzed in different organs and developmental stages via qRT-PCR. Eight genes were significantly up-regulated in young leaves exposed to heat stress at 42 °C, and also showed differential responses to ABA treatment. We also compared expression of these genes with corresponding Arabidopsis sHSP genes and found some differences between the two species.
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Identification and characterization of transcripts differentially expressed during embryogenesis in Capsella Bursa-Pastoris
Biologia Plantarum, 2012Co-Authors: Jianbo WangAbstract:Large-scale gene expression analyses were conducted during embryonic development of Capsella Bursa-Pastoris using the cDNA-AFLP technique, and 231 differentially expressed genes were identified. Most of the transcript-derived fragments (TDFs) were identified against Arabidopsis sequences and a minority against other plant sequences by searching in NCBI database. A total of 128 TDFs, homologous to genes with known functions, were classified into 13 functional categories, and many of them were involved in transcription, metabolism, disease defense and protein binding. Expression profiles of 90 C. Bursa-Pastoris genes were compared with those of the corresponding Arabidopsis genes and 64 genes showed similar expression profiles and the expression profiles of the rest 26 TDFs were different from those of the relevant Arabidopsis genes. Besides, some genes related to oxidative stress and ABA response were fiercely up-regulated during embryogenesis of C. Bursa-Pastoris according to RT-PCR.