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Pung-pung Hwang - One of the best experts on this subject based on the ideXlab platform.

Hua Zhang - One of the best experts on this subject based on the ideXlab platform.

Yung-che Tseng - One of the best experts on this subject based on the ideXlab platform.

Kenzo Nakamura - One of the best experts on this subject based on the ideXlab platform.

  • Two cis-acting regulatory elements are involved in the sucrose-inducible expression of the sporamin gene promoter from sweet potato in transgenic tobacco
    Molecular Genetics and Genomics, 2005
    Co-Authors: Atsushi Morikami, Rie Matsunaga, Yoshimi Tanaka, Satomi Suzuki, Shoji Mano, Kenzo Nakamura
    Abstract:

    In this study, we generated transgenic tobacco plants that express the β-glucuronidase (GUS) gene under the control of the 305-bp 5′-upstream region of a gene coding for sporamin A of sweet potato. Expression of GUS in excised tobacco leaves was induced by sucrose, mimicking the sugar-inducible expression of the endogenous sporamin genes in sweet potato. Deletion of the sequences extending from position −305 (relative to the transcription start site) to −283 and from −146 to −87 resulted in an approximately 40-fold enhancement in GUS reporter expression. Furthermore, the sequence from −282 to −165 conferred sucrose-inducibility on the −89 core promoter of the Cauliflower Mosaic Virus 35S RNA gene. Analysis of internal deletions, linker scanning and the introduction of base substitutions in the sequence between positions −282 and −165 indicated that sucrose-responsiveness conferred by this region was dependent on the presence of two cis -acting elements, termed CMSREs (Carbohydrate Metabolite signal responsive elements) 1 and 2, which are separated by a spacer. A sequence similar or identical to the core of CMSRE-1 (TGGACGG) is also present in the promoters of several other sugar-inducible genes, and sequences encopassing the TGGACGG-related motifs from two of these could functionally replace the CMSRE-1 in the truncated sporamin A promoter. These results suggest that the TGGACGG element plays an important role in the sucrose-inducible expression of a group of plant genes.

Afsar - One of the best experts on this subject based on the ideXlab platform.

  • Glucose Post Exposure Recovery from Lead Intoxicated Freshwater Fish Anabas testudineus
    Journal of Ecophysiology and Occupational Health, 2011
    Co-Authors: Afsar
    Abstract:

    Glucose are important amongst the several molecules available in the cells, Carbohydrates play an important role in the cellular process Under extreme stress conditions, Carbohydrate Metabolite such as glucose have been known to act as the energy supplier in metabolic pathways and biochemical reactions. In the present investigation fish treated with an equitoxic dose of 10 ppm of lead nitrate and lead acetate intoxicated fish After a period of 15 days of exposure a batch from lead nitrate exposed fish and a batch from lead acetate exposed fish were transferred to lead-freewater. Fishes were scarified on 1, 4, 8, 12 and 15 days for the analysis of recovery pattern in tissues viz. liver, muscle, kidney, gill and brain .It is found that lead toxicated fishes were recovered after 15 days depends upon physical condition of fish.