The Experts below are selected from a list of 3138 Experts worldwide ranked by ideXlab platform
Young Wook Choi - One of the best experts on this subject based on the ideXlab platform.
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enhanced topical delivery of tacrolimus by a Carbomer hydrogel formulation with transcutol p
Drug Development and Industrial Pharmacy, 2016Co-Authors: Jong Bu Kang, Dong Woo Yeom, Ho Yub Yoon, Seong Shin Kwak, Young Wook ChoiAbstract:AbstractTacrolimus (TAC), a non-steroidal anti-inflammatory and immunosuppressive agent, is used for the treatment of atopic dermatitis (AD) and skin immune diseases. TAC-loaded topical hydrogel formulations composed of Carbomer, carnosine, transcutol P (diethylene glycol monoethyl ether) and humectant were prepared. For comparison, TAC-loaded topical cream-type formulations were also prepared and commercially available TAC ointment was used as a reference. A drug release study in vitro revealed that the total amount of TAC released from hydrogels over 24 h was approximately 30 times greater than that for the reference formulation. Compared to the reference ointment and creams, Carbomer gel formulations showed higher skin permeation and retention of TAC (significantly different at p < 0.05), especially those with more than 10% of transcutol P. Therefore, Carbomer gel formulations with sufficient levels of transcutol P are good candidates for skin delivery of TAC and have potential as therapeutic agents fo...
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Enhanced topical delivery of tacrolimus by a Carbomer hydrogel formulation with Transcutol P
Drug Development and Industrial Pharmacy, 2016Co-Authors: Jong Bu Kang, Dong Woo Yeom, Ho Yub Yoon, Seong Shin Kwak, Young Wook ChoiAbstract:AbstractTacrolimus (TAC), a non-steroidal anti-inflammatory and immunosuppressive agent, is used for the treatment of atopic dermatitis (AD) and skin immune diseases. TAC-loaded topical hydrogel formulations composed of Carbomer, carnosine, transcutol P (diethylene glycol monoethyl ether) and humectant were prepared. For comparison, TAC-loaded topical cream-type formulations were also prepared and commercially available TAC ointment was used as a reference. A drug release study in vitro revealed that the total amount of TAC released from hydrogels over 24 h was approximately 30 times greater than that for the reference formulation. Compared to the reference ointment and creams, Carbomer gel formulations showed higher skin permeation and retention of TAC (significantly different at p
Li Zhang - One of the best experts on this subject based on the ideXlab platform.
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long term toxicity study of topical administration of a highly stable rh afgf Carbomer 940 hydrogel in a rabbit skin wound model
Frontiers in Pharmacology, 2020Co-Authors: Li Zhang, Tongzhou Huang, Jianing Bi, Yingying Zheng, Chao LuAbstract:: We developed a highly stable recombinant human acidic fibroblast growth factor (rh-aFGF) Carbomer 940 hydrogel for wound healing. This study aimed to reveal toxicity target organs and the toxicity dose-response in the long-term administration of rh-aFGF Carbomer 940 hydrogel in a rabbit skin wound model. New Zealand rabbits were topically administrated rh-aFGF Carbomer 940 hydrogel at a daily dose of 900 IU/cm2, 1,800 IU/cm2, and 3,600 IU/cm2 for 28 days. Lyophilized rh-aFGF agent was used as the positive control group. General behavior, serum chemistry, skin irritation, immunogenicity, immunotoxicity, and histopathology were analyzed at designated time points. Results revealed that food intake, body weight, body temperature, heart rate, and eye examinations were all normal, suggesting no obvious toxicity induced by the rh-aFGF hydrogel. Medium and high dose rh-aFGF hydrogel groups and the positive control group displayed increased cell numbers in the local lymph nodes near the site of administration, likely caused mesenteric lymph node follicular hyperplasia, and this observation was alleviated after 14 days of recovery. Immunogenicity studies demonstrated that the serum antibody titer against rh-aFGF increased with the duration and number of drug applications but were not neutralization antibodies. After administration stopped, antibody titer decreased and disappeared in some mice. In summary, the safe dose for long-term administration of rh-aFGF Carbomer 940 hydrogel for persistently damaged skin was 900 IU/cm2, which is 10 times that of the proposed clinical dosing.
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higher biostability of rh afgf Carbomer 940 hydrogel and its effect on wound healing in a diabetic rat model
ACS Biomaterials Science & Engineering, 2018Co-Authors: Li Zhang, Xuanxin Yang, Bingjie Yu, Zhen Huang, Shucai Pang, Qingde Zhou, Rongshuai Yang, Wenqing Li, Lufeng Hu, Xiaokun LiAbstract:Hydrogels are excellent drug delivery carriers with excellent ductility. Here, we report the design of a higher biostability of a recombinant human acidic fibroblast growth factor (rh-aFGF) Carbomer hydrogel formulation. To verify the optimality of this formula, we prepared various prescriptions and tested the resulting physical properties including micromorphology, long-term stability, accelerated stability, and destructive test. Furthermore, the efficacy for promoting wound healing in full-thickness injury and scald wound diabetic rat models was explored. We found that rh-aFGF-Carbomer hydrogel had good physical properties. It was stable for 24 months at 5 ± 3 °C, and for 6 months at 25 ± 3 °C. In vivo, the rh-aFGF-Carbomer 940 hydrogel achieved a remarkable promotion of skin wound healing in diabetic rats with full-thickness injuries or scald wounds. Our data suggest that rh-aFGF-Carbomer hydrogel may have applications for the treatment of diabetic ulcers combined with other wounds.
Chao Lu - One of the best experts on this subject based on the ideXlab platform.
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long term toxicity study of topical administration of a highly stable rh afgf Carbomer 940 hydrogel in a rabbit skin wound model
Frontiers in Pharmacology, 2020Co-Authors: Li Zhang, Tongzhou Huang, Jianing Bi, Yingying Zheng, Chao LuAbstract:: We developed a highly stable recombinant human acidic fibroblast growth factor (rh-aFGF) Carbomer 940 hydrogel for wound healing. This study aimed to reveal toxicity target organs and the toxicity dose-response in the long-term administration of rh-aFGF Carbomer 940 hydrogel in a rabbit skin wound model. New Zealand rabbits were topically administrated rh-aFGF Carbomer 940 hydrogel at a daily dose of 900 IU/cm2, 1,800 IU/cm2, and 3,600 IU/cm2 for 28 days. Lyophilized rh-aFGF agent was used as the positive control group. General behavior, serum chemistry, skin irritation, immunogenicity, immunotoxicity, and histopathology were analyzed at designated time points. Results revealed that food intake, body weight, body temperature, heart rate, and eye examinations were all normal, suggesting no obvious toxicity induced by the rh-aFGF hydrogel. Medium and high dose rh-aFGF hydrogel groups and the positive control group displayed increased cell numbers in the local lymph nodes near the site of administration, likely caused mesenteric lymph node follicular hyperplasia, and this observation was alleviated after 14 days of recovery. Immunogenicity studies demonstrated that the serum antibody titer against rh-aFGF increased with the duration and number of drug applications but were not neutralization antibodies. After administration stopped, antibody titer decreased and disappeared in some mice. In summary, the safe dose for long-term administration of rh-aFGF Carbomer 940 hydrogel for persistently damaged skin was 900 IU/cm2, which is 10 times that of the proposed clinical dosing.
Huaxi Xu - One of the best experts on this subject based on the ideXlab platform.
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formulation and in vitro stability evaluation of ethosomal Carbomer hydrogel for transdermal vaccine delivery
Colloids and Surfaces B: Biointerfaces, 2018Co-Authors: Yibang Zhang, Wei Beng Ng, Jianguo Hu, Salma Saleh Mussa, Yanru Ge, Huaxi XuAbstract:Abstract The primary objective of this study was to develop, evaluate and compare the effectiveness and stability of ethosomal Carbomer gels in different solvents. The optimal ethosomal formulation was isolated to create ethosomal gels using Carbomer in either pure water (water gel) or PBS containing 30% ethanol (PBS gel). In vitro release of the ethosomal gels were tested using Franz apparatus on hydrophilic and hydrophobic artificial membranes. In vitro stability of two ethosomal gels was systematically evaluated. Transdermal antigen delivery of ethosomal gel was finally performed on the skin of hair removal mice. Both solvent and concentration effects on the in vitro release performance of ethosomal gel of Carbomer have been confirmed. Penetration depth has been found to be dependent on the nature of the membranes such that penetration rate is higher in the hydrophobic membrane than the hydrophilic ones. Furthermore, in vitro stability test indicated that ethosomal PBS gel was more stable than ethosomal water gel. In vivo immunoassay confirmed that the ethosomal PBS gel could deliver the antigenic molecules into the skin of mice and stimulate specific IgG secretion. Using the same solvent for lipid vesicular formulation when making polymeric hydrogel may help to provide a more conducive environment for lipid vesicles and hence enhance their roles in transdermal antigen delivery.
Christophe Baudouin - One of the best experts on this subject based on the ideXlab platform.
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efficacy of 0 18 hypotonic sodium hyaluronate ophthalmic solution in the treatment of signs and symptoms of dry eye disease
Journal Francais D Ophtalmologie, 2012Co-Authors: V Baeyens, Am Bron, Christophe BaudouinAbstract:Summary Background/aims To compare the safety and efficacy of hypotonic 0.18% sodium hyaluronate solution (0.18% SH) versus saline and versus 0.3% Carbomer for the treatment of signs and symptoms of moderate dry eye syndrome. Methods A total of 304 patients were randomized (1:1:1) in this parallel-group, multi-center, phase III trial. They were instructed to instill one drop of the allocated product in each eye two to four times per day over 84 days. The primary efficacy criterion was the change from baseline at Day 28 in symptom frequency score. The superiority of 0.18% SH (Vismed ® ) over saline and its non-inferiority versus Carbomer were statistically tested. Results At Day 28, there was a statistically significant superiority of 0.18% SH over saline in change from baseline for subjective symptom frequency score ( P = 0.0376, primary endpoint) and objective fluorescein staining score ( P = 0.0074, secondary endpoint). 0.18% SH had an excellent safety profile over 84 days. A strong trend was observed in favour of 0.18% SH to cause less blurred vision than Carbomer throughout the trial ( P = 0.0798 at Day 28). Conclusion 0.18% SH caused a statistically significant improvement in both a subjective endpoint (symptom frequency score) and an objective endpoint (fluorescein staining score). 0.18% SH was well tolerated and resulted in low incidence of adverse events.
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cytoprotective effects of hyaluronic acid and Carbomer 934p in ocular surface epithelial cells
Investigative Ophthalmology & Visual Science, 2002Co-Authors: C Debbasch, F Brignole, J M Warnet, Christophe BaudouinAbstract:PURPOSE. To investigate in vitro the cell toxicity and antioxidant effects of two major tear substitutes, hyaluronic acid and a widely used Carbomer, with and without preservative. METHODS. Chang conjunctival cells were treated with different concentrations of unpreserved or preserved Carbomer 934P (0.03% and 0.3%), unpreserved or preserved hyaluronic acid (0.018% and 0.18%), and benzalkonium chloride (BAC 0.0005% and 0.005%) for 15 minutes or for 15 minutes with 24 hours of cell recovery, according to previously validated methods. Microplate cold light cytofluorometry was performed to evaluate cell viability (neutral red test), chromatin condensation (Hoechst 33342 test), and reactive oxygen species (ROS) production (dichlorofluorescein diacetate and hydroethidine tests). Confocal microscopy was used to explore morphologic changes. RESULTS. No alterations were found with unpreserved and preserved hyaluronic acid at all concentrations and times tested. A decrease in cell viability with chromatin condensation appeared with 0.3% preserved Carbomer 934P at the two times tested. This cytotoxicity, however, was significantly less than that observed with BAC alone, although the same concentrations of preservative were used. Unpreserved Carbomer 934P induced no modification of cell viability after 15 minutes but a significant decrease in chromatin condensation, reversible after 24 hours of cell recovery, when a delayed decrease in cell viability was observed. Production of reactive oxygen species (ROS) decreased with the four formulations of tear substitutes tested at their usual concentrations, whereas a significant production of ROS occurred with BAC. CONCLUSIONS. These two ophthalmic hydrogels have no cytotoxicity but possess antioxidant properties and tend to reduce the toxic effects of preservatives. These results may allow use of hydrogels, not only in dry eye but also in ocular surface disorders involving oxidative stress and in ophthalmic drug therapy to improve ocular tolerance. (Invest Ophthalmol Vis Sci. 2002;43:3409 ‐3415)