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Jianlong Wang - One of the best experts on this subject based on the ideXlab platform.
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denitrification performance and microbial diversity in a packed bed bioreactor using pcl as Carbon Source and biofilm carrier
Applied Microbiology and Biotechnology, 2013Co-Authors: Weizhong Wu, Luhua Yang, Jianlong WangAbstract:Polycaprolactone (PCL) was used as both Carbon Source and biofilm support for denitrifying bacteria in a packed-bed bioreactor. The denitrification performance and microbial diversity were investigated. The microbial community of biofilm developed on the surface of PCL in the reactor was analyzed by pyrosequencing method. The experimental results showed the average nitrate removal efficiency reached 93 % at stable operation. ESEM observation and FTIR analysis were conducted to characterize the PCL structure before and after microbial utilization. For the microbial community, Betaproteobacteria predominated, and most of the PCL-degrading denitrifying bacteria assigned to the family of Comamonadacea. Denitrifying bacteria accounted for more than 20 % in the total population, indicating that PCL is a good carrier and Carbon Source for biological denitrification.
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denitrification using pbs as Carbon Source and biofilm support in a packed bed bioreactor
Environmental Science and Pollution Research, 2013Co-Authors: Weizhong Wu, Luhua Yang, Jianlong WangAbstract:Biodegradable polymer was used as Carbon Source and biofilm support for nitrate removal from aqueous solution as an attractive alternative for biological denitrification. The objective of this paper was to investigate the denitrification performance and microbial community of a packed-bed bioreactor using poly (butanediol succinate) (PBS), a biodegradable polymer, as Carbon Source and biofilm support. NO3–N concentration was determined by UV spectrophotometer. NO2–N concentration was assayed by hydrochloric acid naphthyl ethylenediamine spectrophotometry method. Total organic Carbon (TOC) was measured using a TOC analyzer. The morphology of the samples was observed using an environmental scanning electron microscope (ESEM). The microbial community was analyzed by pyrosequencing method. The experimental results showed that an average removal efficiency of nitrate was 95 %. ESEM observation and FTIR analysis indicated the changes of PBS granules before and after microbial utilization. Pyrosequencing results showed that Betaproteobacteria predominated, and most of PBS-degrading denitrifying bacteria were assigned to the family Comamonadaceae. Denitrifying bacteria accounted for 13.02 % in total population. The PBS granules were suitable support and Carbon Source for denitrifying bacteria.
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nitrogen removal using biodegradable polymers as Carbon Source and biofilm carriers in a moving bed biofilm reactor
Chemical Engineering Journal, 2011Co-Authors: Jianlong WangAbstract:Biodegradable polymer (PCL) was used as both solid Carbon Source and biofilm carriers to remove nitrogen from wastewater with a low C/N ratio by simultaneous nitrification and denitrification (SND) in a moving bed biofilm reactor. The experimental results showed that the average TN removal efficiency was 74.6% at hydraulic retention time of 18.5 h. PCL was available both for microbial attachment and for Carbon Source. The consumed PCL amount for removing per gram of TN was calculated to be 1.27 g PCL g−1 N. From the batch tests, 96.3% of nitrogen was removed via SND for the biofilm biomass attached to PCL, compared to 52.0% for the suspended sludge with addition of glucose. This indicated that PCL is an effective solid Carbon Source for simultaneous nitrification and denitrification since it was degraded much slower than soluble substrate which can be rapidly oxidized to CO2 within the early stage of aeration, instead of providing reducing power for denitrification.
Weizhong Wu - One of the best experts on this subject based on the ideXlab platform.
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denitrification performance and microbial diversity in a packed bed bioreactor using pcl as Carbon Source and biofilm carrier
Applied Microbiology and Biotechnology, 2013Co-Authors: Weizhong Wu, Luhua Yang, Jianlong WangAbstract:Polycaprolactone (PCL) was used as both Carbon Source and biofilm support for denitrifying bacteria in a packed-bed bioreactor. The denitrification performance and microbial diversity were investigated. The microbial community of biofilm developed on the surface of PCL in the reactor was analyzed by pyrosequencing method. The experimental results showed the average nitrate removal efficiency reached 93 % at stable operation. ESEM observation and FTIR analysis were conducted to characterize the PCL structure before and after microbial utilization. For the microbial community, Betaproteobacteria predominated, and most of the PCL-degrading denitrifying bacteria assigned to the family of Comamonadacea. Denitrifying bacteria accounted for more than 20 % in the total population, indicating that PCL is a good carrier and Carbon Source for biological denitrification.
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denitrification using pbs as Carbon Source and biofilm support in a packed bed bioreactor
Environmental Science and Pollution Research, 2013Co-Authors: Weizhong Wu, Luhua Yang, Jianlong WangAbstract:Biodegradable polymer was used as Carbon Source and biofilm support for nitrate removal from aqueous solution as an attractive alternative for biological denitrification. The objective of this paper was to investigate the denitrification performance and microbial community of a packed-bed bioreactor using poly (butanediol succinate) (PBS), a biodegradable polymer, as Carbon Source and biofilm support. NO3–N concentration was determined by UV spectrophotometer. NO2–N concentration was assayed by hydrochloric acid naphthyl ethylenediamine spectrophotometry method. Total organic Carbon (TOC) was measured using a TOC analyzer. The morphology of the samples was observed using an environmental scanning electron microscope (ESEM). The microbial community was analyzed by pyrosequencing method. The experimental results showed that an average removal efficiency of nitrate was 95 %. ESEM observation and FTIR analysis indicated the changes of PBS granules before and after microbial utilization. Pyrosequencing results showed that Betaproteobacteria predominated, and most of PBS-degrading denitrifying bacteria were assigned to the family Comamonadaceae. Denitrifying bacteria accounted for 13.02 % in total population. The PBS granules were suitable support and Carbon Source for denitrifying bacteria.
Luhua Yang - One of the best experts on this subject based on the ideXlab platform.
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denitrification performance and microbial diversity in a packed bed bioreactor using pcl as Carbon Source and biofilm carrier
Applied Microbiology and Biotechnology, 2013Co-Authors: Weizhong Wu, Luhua Yang, Jianlong WangAbstract:Polycaprolactone (PCL) was used as both Carbon Source and biofilm support for denitrifying bacteria in a packed-bed bioreactor. The denitrification performance and microbial diversity were investigated. The microbial community of biofilm developed on the surface of PCL in the reactor was analyzed by pyrosequencing method. The experimental results showed the average nitrate removal efficiency reached 93 % at stable operation. ESEM observation and FTIR analysis were conducted to characterize the PCL structure before and after microbial utilization. For the microbial community, Betaproteobacteria predominated, and most of the PCL-degrading denitrifying bacteria assigned to the family of Comamonadacea. Denitrifying bacteria accounted for more than 20 % in the total population, indicating that PCL is a good carrier and Carbon Source for biological denitrification.
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denitrification using pbs as Carbon Source and biofilm support in a packed bed bioreactor
Environmental Science and Pollution Research, 2013Co-Authors: Weizhong Wu, Luhua Yang, Jianlong WangAbstract:Biodegradable polymer was used as Carbon Source and biofilm support for nitrate removal from aqueous solution as an attractive alternative for biological denitrification. The objective of this paper was to investigate the denitrification performance and microbial community of a packed-bed bioreactor using poly (butanediol succinate) (PBS), a biodegradable polymer, as Carbon Source and biofilm support. NO3–N concentration was determined by UV spectrophotometer. NO2–N concentration was assayed by hydrochloric acid naphthyl ethylenediamine spectrophotometry method. Total organic Carbon (TOC) was measured using a TOC analyzer. The morphology of the samples was observed using an environmental scanning electron microscope (ESEM). The microbial community was analyzed by pyrosequencing method. The experimental results showed that an average removal efficiency of nitrate was 95 %. ESEM observation and FTIR analysis indicated the changes of PBS granules before and after microbial utilization. Pyrosequencing results showed that Betaproteobacteria predominated, and most of PBS-degrading denitrifying bacteria were assigned to the family Comamonadaceae. Denitrifying bacteria accounted for 13.02 % in total population. The PBS granules were suitable support and Carbon Source for denitrifying bacteria.
Yongzhen Peng - One of the best experts on this subject based on the ideXlab platform.
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Effects of Carbon Source Types on Denitrification Performance at Low Temperature
Environmental Sciences, 2020Co-Authors: Shuying Wang, Ang Xy, Hou Hx, Yongzhen Peng, Wei WangAbstract:To investigate the responding behavior of denitrifying bacteria to different Carbon Source(i.e.methanol,ethanol,sodium acetate,sodium propionate, glucose,domestic wastewater and endogenous Carbon Source),several batch experiments using the sludge from the Carrousel oxidation ditch were carried out at 15.4℃±0.8℃.The results from sodium acetate feeding experiment show the highest maximum specific denitrification rate(MSDR),which is 6.51(mg/(g·h)).However,the denitrificaion yield,which is 0.48,is at lowest level and an obvious accumulating of nitrite is observed.Compared to the other sole Carbon Source,a lower MSDR,0.91(mg/(g·h)) is shown when methanol is used as Carbon Source,which also suggests that the sufficient adaptation time should be promised for denitrifying bacteria.When the reactor is operated without exogenous Carbon Source,denitrifying bacteria can use the endogenous Carbon Source as electron donor for denitrification,but the specific denitrification rate is at lowest level accordingly.The MSDR value of 3.63(mg/(g·h)) is obtained with fermented domestic wastewater feeding,which is equivalent to the MSDR attained from the test using VFAs as Carbon Source.Though the MSDR decreases a lot at low temperature,external Carbon Source addition improves the denitrification performance to some extent definitely.
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Effect of Carbon Source type on intracellular stored polymers during endogenous denitritation (ED) treating landfill leachate.
Water Research, 2016Co-Authors: Lei Miao, Shuying Wang, Baikun Li, Fangzhai Zhang, Zhong Wang, Yongzhen PengAbstract:Abstract Glycogen accumulating organisms (GAOs) capable of storing organic compounds as polyhydroxyalkanoate (PHA) have been used for endogenous denitritation (ED), but the effect of Carbon Sources type on nitrogen removal performance of GAOs treating landfill leachate is unclear. In this study, a successful ED system treating landfill leachate (COD/NH 4 + -N (C/N): 4) without external Carbon Source addition was applied. The mature leachate with C/N of 1 was used as the feeding base solution, with acetate, propionate, and glucose examined as the Carbon Sources, and their effects on yields and compositions of PHA produced by GAOs were determined and associated with nitrogen removal performance. In the case of sole Carbon Source, acetate was much easier to be stored than propionate and glucose, which led to a higher nitrogen removal efficiency. Glucose had the lowest amount of PHA storage and led to the lowest performance. In the case of composite Carbon Sources (two scenarios: acetate + propionate; acetate + propionate + glucose), GAOs stored sufficient PHA and exhibited similar nitrogen removal efficiencies. Moreover, type of Carbon Source influenced the compositions of PHA. The polyhydroxybutyrate (PHB) fraction in PHA was far more than polyhydroxyvalerate (PHV) in all tests. PHV was synthesized only when acetate existed in Carbon Source. The microbial diversity analysis revealed that Proteobacteria was the most abundant phylum. Among the 108 genera detected in this ED system, the genera responsible for denitritation were Thauera , Paracoccus , Ottowia and Comamonadaceae_unclassified , accounting for 46.21% of total bacteria. Especially, Paracoccus and Comamonadaceae_unclassified transformed the Carbon Source into PHA for denitritation, and carried out endogenous denitritation.
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free nitrous acid pretreatment of wasted activated sludge to exploit internal Carbon Source for enhanced denitrification
Bioresource Technology, 2015Co-Authors: Yongzhen Peng, Baikun Li, Yayi WangAbstract:Abstract Using internal Carbon Source contained in waste activated sludge (WAS) is beneficial for nitrogen removal from wastewater with low Carbon/nitrogen ratio, but it is usually limited by sludge disintegration. This study presented a novel strategy based on free nitrous acid (FNA) pretreatment to intensify the release of organic matters from WAS for enhanced denitrification. During FNA pretreatment, soluble chemical oxygen demand (SCOD) production kept increasing when FNA increased from 0 to 2.04 mg HNO2-N/L. Compared with untreated WAS, the internal Carbon Source production increased by 50% in a simultaneous fermentation and denitrification reactor fed with WAS pretreated by FNA for 24 h at 2.04 mg HNO2-N/L. This also increased denitrification efficiency by 76% and sludge reduction by 87.5%. More importantly, greenhouse gas nitrous oxide production in denitrification was alleviated since more electrons could be provided by FNA pretreated WAS.
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Effects of Different Carbon Source on the Performance and Microbial Community of EBPR during Start-Up Period
2012 2nd International Conference on Remote Sensing Environment and Transportation Engineering, 2012Co-Authors: Yongzhen Peng, Shuying Wang, Zhi-jia Miao, Dong-chen WengAbstract:In order to analysis the impact of different Carbon Source on the performance and microbial community of Enhanced biological phosphorus removal (EBPR) start-up period, 4 laboratory scale sequencing batch reactors (SBR) supplied with different Carbon Source were operated. SBR1 (fed with switched Carbon Source between acetate and propionate) quickly achieved enhanced phosphorus removal efficiency, the phosphate removal rate reached 95% at 50th day, while SBR2(fed with glucose as a sole Carbon Source) still remained about 50% at the same time. DGGE analysis revealed the number of major bands of SBR1 and SBR2 decreased during the start-up period, thus their microbial community were getting simpler. The quantity of populations of PAOs and GAOs in each SBR was detected with FISH, it was revealed that PAOs increased rapidly in SBR1 on 27th d and 50th d, and finally reached 57%. Population of PAOs in SBR2 reached 20% on 42th d and kept stability. While only 7% and 4.5% appeared in SBR3 and SBR4, respectively. This study showed that different Carbon Source had a significant impact on the microbial community structure succession, and Carbon Source switched between acetate and propionate had significant advantage over glucose and waste-water in the EBPR start-up period.
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Influence of Carbon Source on EBPR metabolism and microorganism communities
Environmental Sciences, 2009Co-Authors: C.-y. Wu, Yongzhen Peng, Xiaoli Li, Zhiguo YuanAbstract:A SBR was used in this study for investigating the influence of Carbon Source on EBPR metabolism and microorganism communities when feeding with acetate and propionate. The SBR was operated with a cycle time of 8 h and each cycle consisted of 4 min feeding, 2 h anaerobic period, 5 h aerobic period, 35 min setting, 15 min decanting and 6 min waiting. The COD of influent was kept at 300 mg/L during the experiment. Acetate and propionate were used as the sole Carbon Source for operation of 60 days, respectively. The phosphorus release/COD consumption in the end of anaerobic phase were 0.35 and 0.27 when acetate and propionate were used as the Carbon Source, respectively. The PHA composition was different when different Carbon Source was dosed. PHB accounted for 92.6% in the end of anaerobic phase but the value for PHV was only 7.4% when acetate was selected as the Carbon Source. No PH2MV was detected during this process. The compositions of PHA were PHB (10.2%), PHV (35.8%) and PH2MV (54.0%) in the end of anaerobic cycle when propionate was used as the sole Carbon Source. There was variation of microorganism communities during this process for the results of DGGE combined with SEM micrographs and PHA staining. Coccus morphotype PAOs were accumulated in acetate-fed phase and rod morphotype PAOs were accumulated in propionate-fed stage. Different PAOs were accumulated and the metabolic pathways were different when different Carbon Sources were used, but good EBPR could be achieved during all these conditions.
Bikem Ovez - One of the best experts on this subject based on the ideXlab platform.
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biological denitrification in drinking water using glycyrrhiza glabra and arunda donax as the Carbon Source
Process Biochemistry, 2006Co-Authors: Bikem Ovez, S Ozgen, M YukselAbstract:Abstract In this research, two natural organic substances; liquorice ( Glycyrrhiza glabra ) and giant reed ( Arundo donax ) were investigated as a Carbon Source in the biological denitrification of drinking water. These materials act as a solid substrate and bio-film carrier. The experiments were carried out in batch, semi-batch and continuous processes. Complete denitrification was achieved with G. glabra . The removal efficiency of A. donax varied between 87 and 100% according to the type of process used. It was found that the nitrate removal rate of G. glabra was 6.96 mg/(L day) N-NO 3 and the nitrate removal rate of A. donax was 4.23 mg/(L day) N-NO 3 . Results showed that these organic substrates could be used as an alternative Carbon Source for denitrification with complex process control and continuous monitoring. It should be noted that Carbon Source should be changed periodically for the continuation of the process.
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batch biological denitrification using arundo donax glycyrrhiza glabra and gracilaria verrucosa as Carbon Source
Process Biochemistry, 2006Co-Authors: Bikem OvezAbstract:Different natural organic solid substances (NOSS) were examined as the Carbon Source for biological denitrification in batch reactors. Among the examined substances, Gracilaria verrucosa, liquorice, and giant reed gave positive response to denitrification and complete nitrate removal was achieved. Throughout the study, the effect of inoculum addition, pH, and Carbon amount change on denitrification was also investigated. Results showed that best denitrification performance was achieved with G. verrucosa with the highest protein amount and its large surface area.