The Experts below are selected from a list of 6945 Experts worldwide ranked by ideXlab platform

Chengming Hsu - One of the best experts on this subject based on the ideXlab platform.

  • autologous thyroid Cartilage Graft implantation in medialization laryngoplasty a modified approach for treating unilateral vocal fold paralysis
    Scientific Reports, 2017
    Co-Authors: Mingshao Tsai, Mingyu Yang, Genghe Chang, Yaote Tsai, Menghung Lin, Chengming Hsu
    Abstract:

    Medialization laryngoplasty is the standard surgical treatment for unilateral vocal fold paralysis. This study presents a modified approach in which a thyroid Cartilage Graft is implanted in medialization laryngoplasty. 22 patients who underwent this approach were included in the study. The results revealed that glottal incompetence and vocal performance were markedly improved following surgery, and the follow-up period ranged from 6 to 74 months (mean, 21.4 months). Acoustic analysis revealed significant improvements in the maximum phonation time (from 3.51 to 7.89 seconds, p < 0.001), F0 (from 221.7 to 171.0 Hertz, p = 0.025), and jitter (from 7.68 to 3.19, p < 0.001). Perceptual assessment revealed a significant decrease in voice grading (from 2.59 to 1.41, p < 0.001), roughness (from 1.82 to 1.23, p = 0.004), and voice breathiness (from 2.55 to 1.23, p < 0.001). None of the patients exhibited severe wound infection, tissue rejection, or other complications attributed to the surgical procedure. In conclusion, autologous thyroid Cartilage implantation in medialization laryngoplasty medializes the vocal cord, minimizes the glottal gap, and improves the voice of patients with vocal fold paralysis. This procedure is characterized by simplicity, safety, and acceptable results.

Dongan Wang - One of the best experts on this subject based on the ideXlab platform.

  • decellularized tissue engineered hyaline Cartilage Graft for articular Cartilage repair
    Biomaterials, 2020
    Co-Authors: Xiaolei Nie, Yon Jin Chuah, Wenzhen Zhu, Yvonne Peck, Dongan Wang
    Abstract:

    Abstract Articular Cartilage repair has been a long-standing challenge in orthopaedic medicine due to the limited self-regenerative capability of Cartilage tissue. Currently, Cartilage lesions are often treated by microfracture or autologous chondrocyte implantation (ACI). However, these treatments are frequently reported to result in a mixture of the desired hyaline Cartilage and mechanically inferior fibroCartilage. In this study, by combining the advantages of Cartilage tissue engineering and decellularization technology, we developed a decellularized allogeneic hyaline Cartilage Graft, named dLhCG, which achieved superior efficacy in articular Cartilage repair and surpassed living autologous chondrocyte-based cartilaginous enGraftment and ACI. By the 6-month time point after implantation in porcine knee joints, the fine morphology, composition, phenotype, microstructure and mechanical properties of the regenerated hyaline-like cartilaginous neo-tissue have been demonstrated via histology, biochemical assays, DNA microarrays and mechanical tests. The articular cartilaginous enGraftment with allogeneic dLhCG was indicated to be well consistent, compatible and integrated with the native Cartilage of the host. The successful repair of articular chondral defects in large animal models suggests the readiness of allogeneic dLhCG for clinical trials.

  • a transduced living hyaline Cartilage Graft releasing transgenic stromal cell derived factor 1 inducing endogenous stem cell homing in vivo
    Tissue Engineering Part A, 2013
    Co-Authors: Feng Zhang, Wenyan Leong, Kai Su, Yu Fang, Dongan Wang
    Abstract:

    Stromal cell-derived factor-1 (SDF-1), also known as a homing factor, is a potent chemokine that activates and directs mobilization, migration, and retention of certain cell species via systemic circulation. The responding homing cells largely consist of activated stem cells, so that, in case of tissue lesions, such SDF-1-induced cell migration may execute recruitment of endogenous stem cells to perform autoreparation and compensatory regeneration in situ. In this study, a recombinant adenoviral vector carrying SDF-1 transgene was constructed and applied to transduce a novel scaffold-free living hyaline Cartilage Graft (SDF-t-LhCG). As an engineered transgenic living tissue, SDF-t-LhCG is capable of continuously producing and releasing SDF-1 in vitro and in vivo. The in vitro trials were examined with ELISA, while the in vivo trials were subsequently performed via a subcutaneous implantation of SDF-t-LhCG in a nude mouse model, followed by series of biochemical and biological analyses. The results indicate that transgenic SDF-1 enhanced the presence of this chemokine in mouse's circulation system; in consequence, SDF-1-induced activation and recruitment of endogenous stem cells were also augmented in both peripheral blood and SDF-t-LhCG implant per se. These results were obtained via flow cytometry analyses on mouse blood samples and implanted SDF-t-LhCG samples, indicating an upregulation of the CXCR4+(SDF-1 receptor) cell population, accompanied by upregulation of the CD34+, CD44+, and Sca-1+ cell populations as well as a downregulation of the CD11b+ cell population. With the supply of SDF-1-recruited endogenous stem cells, enhanced chondrogenesis was observed in SDF-t-LhCG implants in situ.

  • creating a living hyaline Cartilage Graft free from non cartilaginous constituents an intermediate role of a biomaterial scaffold
    Advanced Functional Materials, 2012
    Co-Authors: Kai Su, Wenyan Leong, Yihong Gong, Dongan Wang
    Abstract:

    A novel living hyaline Cartilage Graft (LhCG) with controllable dimensions and free of non-cartilaginous constituents for articular regeneration is developed. As a living Graft for regenerative medicine, LhCG is purely living tissue based and truly scaffold-free. The process of neotissue formation in LhCG is mediated by an interim biomaterial-based novel scaffolding system. This design highlights a philosophy of using biomaterials in engineered regenerative medicine as a transient guiding facility rather than a permanent part of substitute. The fabrication is designed and practiced in a continuous and integrated process, which attributes to its simplicity in operation. Because of the intrinsic non-cell-adhesive property of hydrogel scaffolds, articular chondrocytes’ phenotype is always preserved throughout the whole procedure, which has been tested and approved both in vitro and in vivo. In situ Grafting trials in a rabbit model showcase high success rates in both Cartilage repair and Graft-host integration. Beyond Cartilage repair, this LhCG model may provide a living-tissue-based open platform or niche for multi-tissue regenerations.

Mingshao Tsai - One of the best experts on this subject based on the ideXlab platform.

  • autologous thyroid Cartilage Graft implantation in medialization laryngoplasty a modified approach for treating unilateral vocal fold paralysis
    Scientific Reports, 2017
    Co-Authors: Mingshao Tsai, Mingyu Yang, Genghe Chang, Yaote Tsai, Menghung Lin, Chengming Hsu
    Abstract:

    Medialization laryngoplasty is the standard surgical treatment for unilateral vocal fold paralysis. This study presents a modified approach in which a thyroid Cartilage Graft is implanted in medialization laryngoplasty. 22 patients who underwent this approach were included in the study. The results revealed that glottal incompetence and vocal performance were markedly improved following surgery, and the follow-up period ranged from 6 to 74 months (mean, 21.4 months). Acoustic analysis revealed significant improvements in the maximum phonation time (from 3.51 to 7.89 seconds, p < 0.001), F0 (from 221.7 to 171.0 Hertz, p = 0.025), and jitter (from 7.68 to 3.19, p < 0.001). Perceptual assessment revealed a significant decrease in voice grading (from 2.59 to 1.41, p < 0.001), roughness (from 1.82 to 1.23, p = 0.004), and voice breathiness (from 2.55 to 1.23, p < 0.001). None of the patients exhibited severe wound infection, tissue rejection, or other complications attributed to the surgical procedure. In conclusion, autologous thyroid Cartilage implantation in medialization laryngoplasty medializes the vocal cord, minimizes the glottal gap, and improves the voice of patients with vocal fold paralysis. This procedure is characterized by simplicity, safety, and acceptable results.

Mark E Gerber - One of the best experts on this subject based on the ideXlab platform.

Thomas Verse - One of the best experts on this subject based on the ideXlab platform.

  • management of radiation induced tracheocutaneous tissue defects by transplantation of an ear Cartilage Graft and deltopectoral flap
    Auris Nasus Larynx, 2006
    Co-Authors: Frank Riedel, Ulrich R Goessler, Stephan Grupp, Gregor Bran, Karl Hormann, Thomas Verse
    Abstract:

    Patients suffering from head and neck cancer often require temporary tracheostomy during therapy. The tracheostomy can usually be closed when postoperative swelling decreases and swallowing ability recovers. However, some patients, especially after adjuvant radiotherapy, may develop severe chronic wounds resulting in persistent tracheocutaneous fistula. Local wound care and plastic reconstruction strategies are required in such cases. We present two patients with head and neck cancer treated with primary surgical regimen including temporary tracheostomy and adjuvant radiotherapy. Both patients developed a persistent, poorly healing wound with persistent tracheocutaneous tissue defect. After local debridement and wound care, the peristomal necrotic tissue was excised down to the level of the trachea. The defect of the anterior tracheal wall was closed with a autogenous ear Cartilage Graft. The Graft was harvested from the cavum conchae and sutured to the tracheal defect. The soft tissue defect was covered by transposition of a well-vascularized, fasciocutaneous deltopectoral flap. In both cases, the flaps healed satisfactorily. The donor defect was closed primarily. Complications were not observed in these two cases. A flexible tracheo-bronchoscopy showed no stenosis of the trachea at the site of Cartilage Graft transplantation. In conclusion, treatment of persistent radiated tracheocutaneous defects by Cartilage Graft and deltopectoral flap turned out to be a safe and reliable procedure which can be performed as a one-stage method with low morbidity at the donor site.

  • reconstruction of anterior nasal septum back to back autogenous ear Cartilage Graft
    Laryngoscope, 2004
    Co-Authors: Wolfgang Pirsig, Eugene B Kern, Thomas Verse
    Abstract:

    Objectives/Hypothesis The ideal material for reconstructing the nasal septum in the deficient nose has not been found. Since 1986, the authors have used autogenous Cartilage from the cavum conchae to successfully correct the anterior septum and the associated cartilaginous saddle. The long-term results in 26 patients with a destroyed septum and a saddle nose are reported. Study Design Retrospective study. Methods The mean age of the patients at surgery was 40.2 years, and the mean number of previous nasal procedures was 1.6. Because 11 patients had septal perforations and insufficient septal Cartilage or bone, ear Cartilage Grafts from the cavum conchae were harvested through an anterolateral approach. A special incision was used to divide the concave ear Cartilage while preserving the posterior perichondrium. This produced a stable, balanced back-to-back Graft. The Graft was 2.5 to 3 cm long, long enough to allow reconstruction of the anterior septum and to correct part of the saddle nose deformity. The rest of the conchal Cartilage was used to fill the remaining cartilaginous saddle. Follow-up investigations included photographs and visual analogue scales of the patients' symptoms and satisfaction. Results After a mean interval of 36.7 ± 22.3 months, the back-to-back Grafts showed no macroscopic signs of resorption. Graft position and shape remained intact after transplantation. All noses were adequately projected and mobile. All patients but one were satisfied with the functional and esthetic result. With a score of 4 representing the level of satisfaction as “very good,” the mean score of the patients was 3.2 ± 0.79. The saddle of the nose completely disappeared in 65.4% of patients, was minimally visible in 23.1%, and was slightly present in 11.5%. Nasal breathing improved considerably in 21 patients, remained the same in 4 patients, and worsened in 1 patient. The mean score of all patients for nasal breathing was 7.3 ± 1.87 on a visual analogue scale of 0 to 10, with 10 representing satisfaction as “very good”. Conclusion The back-to-back autogenous ear Cartilage Graft is a viable, stable, and balanced Graft for functional and aesthetic reconstruction of the anterior nasal septum and cartilaginous saddle deformity in patients with a severely traumatized and deficient septum.