The Experts below are selected from a list of 5505 Experts worldwide ranked by ideXlab platform

Shigehiko Suzuki - One of the best experts on this subject based on the ideXlab platform.

  • Identification of Cartilage progenitor cells in the adult ear perichondrium: utilization for Cartilage Reconstruction
    Laboratory Investigation, 2006
    Co-Authors: Takeshi Togo, Motoko Naitoh, Yasumi Tsuji, Noriyuki Morikawa, Masayoshi Ohta, Atsushi Utani, Motonobu Nakamura, Shigehiko Suzuki
    Abstract:

    For Cartilage Reconstruction, it is still difficult to obtain a sufficient volume of Cartilage and to maintain its functional phenotype for a long period. Utilizing tissue stem cells is one approach to overcome such difficulties. We show here the presence of Cartilage progenitor cells in the ear perichondrium of adult rabbits by 5-bromo-2′-deoxyuridine labeling, clonogenicity, and differentiation analyses. Long-term label-retaining cells were demonstrated in the perichondrium. Cells from the perichondrium, that is, perichondrocytes were mechanically isolated using a raspatory and maintained in D-MEM/F-12 medium with 10% FCS. They proliferated more vigorously than chondrocytes from the Cartilage. Perichondrocytes could differentiate into adipocytes as well as osteocytes in differentiation induction medium. For Cartilage Reconstruction in vivo , perichondrocytes were seeded on collagen sponge scaffolds and implanted in nude mice. After 4 weeks, the composites with perichondrocytes generated the same weight of cartilaginous tissue as those with chondrocytes. They produced glycosaminoglycan and type II collagen as shown by RT-PCR and immunohistochemical examination. On the contrary, rabbit bone marrow mesenchymal stem cells used as control could regenerate significantly smaller Cartilage than perichondrocytes in the implant study. Based on these findings, we propose that the perichondrium containing tissue progenitor cells is one of the potential candidates for use in reconstructing Cartilage and new therapeutic modalities.

  • identification of Cartilage progenitor cells in the adult ear perichondrium utilization for Cartilage Reconstruction
    Laboratory Investigation, 2006
    Co-Authors: Takeshi Togo, Motoko Naitoh, Yasumi Tsuji, Noriyuki Morikawa, Masayoshi Ohta, Atsushi Utani, Motonobu Nakamura, Shigehiko Suzuki
    Abstract:

    Identification of Cartilage progenitor cells in the adult ear perichondrium: utilization for Cartilage Reconstruction

Biman B. Mandal - One of the best experts on this subject based on the ideXlab platform.

  • potential of agarose silk fibroin blended hydrogel for in vitro Cartilage tissue engineering
    ACS Applied Materials & Interfaces, 2016
    Co-Authors: Yogendra Pratap Singh, Nandana Bhardwaj, Biman B. Mandal
    Abstract:

    An osteoarthritis pandemic has accelerated exploration of various biomaterials for Cartilage Reconstruction with a special emphasis on silk fibroin from mulberry (Bombyx mori) and non-mulberry (Antheraea assamensis) silk worms. Retention of positive attributes of the agarose standard and nullification of its negatives are central to the current agarose/silk fibroin hydrogel design. In this study, hydrogels of mulberry and non-mulberry silk fibroin blended with agarose were fabricated and evaluated in vitro for two weeks for cartilaginous tissue formation. The fabricated hydrogels were physicochemically characterized and analyzed for cell viability, proliferation, and extra cellular matrix deposition. The amalgamation of silk fibroin with agarose impacted the pore size, as illustrated by field emission scanning electron microscopy studies, swelling behavior, and in vitro degradation of the hydrogels. Fourier transform infrared spectroscopy results indicated the blend formation and confirmed the presence of...

  • Potential of Agarose/Silk Fibroin Blended Hydrogel for in Vitro Cartilage Tissue Engineering
    2016
    Co-Authors: Yogendra Pratap Singh, Nandana Bhardwaj, Biman B. Mandal
    Abstract:

    An osteoarthritis pandemic has accelerated exploration of various biomaterials for Cartilage Reconstruction with a special emphasis on silk fibroin from mulberry (Bombyx mori) and non-mulberry (Antheraea assamensis) silk worms. Retention of positive attributes of the agarose standard and nullification of its negatives are central to the current agarose/silk fibroin hydrogel design. In this study, hydrogels of mulberry and non-mulberry silk fibroin blended with agarose were fabricated and evaluated in vitro for two weeks for cartilaginous tissue formation. The fabricated hydrogels were physicochemically characterized and analyzed for cell viability, proliferation, and extra cellular matrix deposition. The amalgamation of silk fibroin with agarose impacted the pore size, as illustrated by field emission scanning electron microscopy studies, swelling behavior, and in vitro degradation of the hydrogels. Fourier transform infrared spectroscopy results indicated the blend formation and confirmed the presence of both components in the fabricated hydrogels. Rheological studies demonstrated enhanced elasticity of blended hydrogels with G′ > G″. Biochemical analysis revealed significantly higher levels of sulfated glycosaminoglycans (sGAGs) and collagen (p ≤ 0.01) in blended hydrogels. More specifically, the non-mulberry silk fibroin blend showed sGAG and collagen content (∼1.5-fold) higher than that of the mulberry blend (p ≤ 0.05). Histological and immunohistochemical analyses further validated the enhanced deposition of sGAG and collagen, indicating maintenance of chondrogenic phenotype within constructs after two weeks of culture. Real-time PCR analysis further confirmed up-regulation of Cartilage-specific aggrecan, sox-9 (∼1.5-fold) and collagen type II (∼2-fold) marker genes (p ≤ 0.01) in blended hydrogels. The hydrogels demonstrated immunocompatibility, which was evidenced by minimal in vitro secretion of tumor necrosis factor-α (TNF-α) by murine macrophages. Taken together, the results suggest promising attributes of blended hydrogels and particularly the non-mulberry silk fibroin/agarose blends as alternative biomaterial for Cartilage tissue engineering

Takeshi Togo - One of the best experts on this subject based on the ideXlab platform.

  • Identification of Cartilage progenitor cells in the adult ear perichondrium: utilization for Cartilage Reconstruction
    Laboratory Investigation, 2006
    Co-Authors: Takeshi Togo, Motoko Naitoh, Yasumi Tsuji, Noriyuki Morikawa, Masayoshi Ohta, Atsushi Utani, Motonobu Nakamura, Shigehiko Suzuki
    Abstract:

    For Cartilage Reconstruction, it is still difficult to obtain a sufficient volume of Cartilage and to maintain its functional phenotype for a long period. Utilizing tissue stem cells is one approach to overcome such difficulties. We show here the presence of Cartilage progenitor cells in the ear perichondrium of adult rabbits by 5-bromo-2′-deoxyuridine labeling, clonogenicity, and differentiation analyses. Long-term label-retaining cells were demonstrated in the perichondrium. Cells from the perichondrium, that is, perichondrocytes were mechanically isolated using a raspatory and maintained in D-MEM/F-12 medium with 10% FCS. They proliferated more vigorously than chondrocytes from the Cartilage. Perichondrocytes could differentiate into adipocytes as well as osteocytes in differentiation induction medium. For Cartilage Reconstruction in vivo , perichondrocytes were seeded on collagen sponge scaffolds and implanted in nude mice. After 4 weeks, the composites with perichondrocytes generated the same weight of cartilaginous tissue as those with chondrocytes. They produced glycosaminoglycan and type II collagen as shown by RT-PCR and immunohistochemical examination. On the contrary, rabbit bone marrow mesenchymal stem cells used as control could regenerate significantly smaller Cartilage than perichondrocytes in the implant study. Based on these findings, we propose that the perichondrium containing tissue progenitor cells is one of the potential candidates for use in reconstructing Cartilage and new therapeutic modalities.

  • identification of Cartilage progenitor cells in the adult ear perichondrium utilization for Cartilage Reconstruction
    Laboratory Investigation, 2006
    Co-Authors: Takeshi Togo, Motoko Naitoh, Yasumi Tsuji, Noriyuki Morikawa, Masayoshi Ohta, Atsushi Utani, Motonobu Nakamura, Shigehiko Suzuki
    Abstract:

    Identification of Cartilage progenitor cells in the adult ear perichondrium: utilization for Cartilage Reconstruction

Atsushi Utani - One of the best experts on this subject based on the ideXlab platform.

  • Identification of Cartilage progenitor cells in the adult ear perichondrium: utilization for Cartilage Reconstruction
    Laboratory Investigation, 2006
    Co-Authors: Takeshi Togo, Motoko Naitoh, Yasumi Tsuji, Noriyuki Morikawa, Masayoshi Ohta, Atsushi Utani, Motonobu Nakamura, Shigehiko Suzuki
    Abstract:

    For Cartilage Reconstruction, it is still difficult to obtain a sufficient volume of Cartilage and to maintain its functional phenotype for a long period. Utilizing tissue stem cells is one approach to overcome such difficulties. We show here the presence of Cartilage progenitor cells in the ear perichondrium of adult rabbits by 5-bromo-2′-deoxyuridine labeling, clonogenicity, and differentiation analyses. Long-term label-retaining cells were demonstrated in the perichondrium. Cells from the perichondrium, that is, perichondrocytes were mechanically isolated using a raspatory and maintained in D-MEM/F-12 medium with 10% FCS. They proliferated more vigorously than chondrocytes from the Cartilage. Perichondrocytes could differentiate into adipocytes as well as osteocytes in differentiation induction medium. For Cartilage Reconstruction in vivo , perichondrocytes were seeded on collagen sponge scaffolds and implanted in nude mice. After 4 weeks, the composites with perichondrocytes generated the same weight of cartilaginous tissue as those with chondrocytes. They produced glycosaminoglycan and type II collagen as shown by RT-PCR and immunohistochemical examination. On the contrary, rabbit bone marrow mesenchymal stem cells used as control could regenerate significantly smaller Cartilage than perichondrocytes in the implant study. Based on these findings, we propose that the perichondrium containing tissue progenitor cells is one of the potential candidates for use in reconstructing Cartilage and new therapeutic modalities.

  • identification of Cartilage progenitor cells in the adult ear perichondrium utilization for Cartilage Reconstruction
    Laboratory Investigation, 2006
    Co-Authors: Takeshi Togo, Motoko Naitoh, Yasumi Tsuji, Noriyuki Morikawa, Masayoshi Ohta, Atsushi Utani, Motonobu Nakamura, Shigehiko Suzuki
    Abstract:

    Identification of Cartilage progenitor cells in the adult ear perichondrium: utilization for Cartilage Reconstruction

Motoko Naitoh - One of the best experts on this subject based on the ideXlab platform.

  • Identification of Cartilage progenitor cells in the adult ear perichondrium: utilization for Cartilage Reconstruction
    Laboratory Investigation, 2006
    Co-Authors: Takeshi Togo, Motoko Naitoh, Yasumi Tsuji, Noriyuki Morikawa, Masayoshi Ohta, Atsushi Utani, Motonobu Nakamura, Shigehiko Suzuki
    Abstract:

    For Cartilage Reconstruction, it is still difficult to obtain a sufficient volume of Cartilage and to maintain its functional phenotype for a long period. Utilizing tissue stem cells is one approach to overcome such difficulties. We show here the presence of Cartilage progenitor cells in the ear perichondrium of adult rabbits by 5-bromo-2′-deoxyuridine labeling, clonogenicity, and differentiation analyses. Long-term label-retaining cells were demonstrated in the perichondrium. Cells from the perichondrium, that is, perichondrocytes were mechanically isolated using a raspatory and maintained in D-MEM/F-12 medium with 10% FCS. They proliferated more vigorously than chondrocytes from the Cartilage. Perichondrocytes could differentiate into adipocytes as well as osteocytes in differentiation induction medium. For Cartilage Reconstruction in vivo , perichondrocytes were seeded on collagen sponge scaffolds and implanted in nude mice. After 4 weeks, the composites with perichondrocytes generated the same weight of cartilaginous tissue as those with chondrocytes. They produced glycosaminoglycan and type II collagen as shown by RT-PCR and immunohistochemical examination. On the contrary, rabbit bone marrow mesenchymal stem cells used as control could regenerate significantly smaller Cartilage than perichondrocytes in the implant study. Based on these findings, we propose that the perichondrium containing tissue progenitor cells is one of the potential candidates for use in reconstructing Cartilage and new therapeutic modalities.

  • identification of Cartilage progenitor cells in the adult ear perichondrium utilization for Cartilage Reconstruction
    Laboratory Investigation, 2006
    Co-Authors: Takeshi Togo, Motoko Naitoh, Yasumi Tsuji, Noriyuki Morikawa, Masayoshi Ohta, Atsushi Utani, Motonobu Nakamura, Shigehiko Suzuki
    Abstract:

    Identification of Cartilage progenitor cells in the adult ear perichondrium: utilization for Cartilage Reconstruction