The Experts below are selected from a list of 321 Experts worldwide ranked by ideXlab platform

Xiaodie Zhang - One of the best experts on this subject based on the ideXlab platform.

  • the in vivo chondrogenesis of cartilage stem progenitor cells from auricular cartilage and the Perichondrium
    American Journal of Translational Research, 2019
    Co-Authors: Xiaodie Zhang, Lin Qi, Yahong Chen, Zhezheng Xiong, Junjie Li, Peng Xu, Huizhong Zhang, Zhuxin Chen
    Abstract:

    Bone marrow-derived stem cells are commonly studied for cartilage tissue engineering and regeneration medicine applications, but their ossification tendency and their limited capacity for chondrogenic differentiation depending on the donor age limit their clinical application. Cartilage stem/progenitor cells are ideal seeding cells, as cartilage stem/progenitor cells from auricular cartilage and the Perichondrium have the inherent advantages of chondrogenesis capacity and an easy and nontraumatic harvesting process, displaying promise for applications. The identification and comparison of cartilage stem/progenitor cells from auricular cartilage and the Perichondrium in vitro were explored in our previous study, but the in vivo chondrogenesis of these cells has not been fully examined. In the current study, we explored the ectopic chondrogenesis of cartilage stem progenitor/cells from auricular cartilage and the Perichondrium after chondrogenic induction in vitro. Our results suggest that stem/progenitor cells from auricular cartilage exhibit significantly better chondrogenesis than those from the Perichondrium in vivo, with upregulated chondrogenic genes and a stable cartilage phenotype, as well as good mechanical properties, indicating that stem/progenitor cells from auricular cartilage could be one type of ideal seeding cells for cartilage tissue engineering.

  • The in vivo chondrogenesis of cartilage stem/progenitor cells from auricular cartilage and the Perichondrium.
    American Journal of Translational Research, 2019
    Co-Authors: Xiaodie Zhang, Lin Qi, Yahong Chen, Zhezheng Xiong, Junjie Li, Peng Xu, Huizhong Zhang, Zhuxin Chen
    Abstract:

    Bone marrow-derived stem cells are commonly studied for cartilage tissue engineering and regeneration medicine applications, but their ossification tendency and their limited capacity for chondrogenic differentiation depending on the donor age limit their clinical application. Cartilage stem/progenitor cells are ideal seeding cells, as cartilage stem/progenitor cells from auricular cartilage and the Perichondrium have the inherent advantages of chondrogenesis capacity and an easy and nontraumatic harvesting process, displaying promise for applications. The identification and comparison of cartilage stem/progenitor cells from auricular cartilage and the Perichondrium in vitro were explored in our previous study, but the in vivo chondrogenesis of these cells has not been fully examined. In the current study, we explored the ectopic chondrogenesis of cartilage stem progenitor/cells from auricular cartilage and the Perichondrium after chondrogenic induction in vitro. Our results suggest that stem/progenitor cells from auricular cartilage exhibit significantly better chondrogenesis than those from the Perichondrium in vivo, with upregulated chondrogenic genes and a stable cartilage phenotype, as well as good mechanical properties, indicating that stem/progenitor cells from auricular cartilage could be one type of ideal seeding cells for cartilage tissue engineering.

  • isolation identification and comparison of cartilage stem progenitor cells from auricular cartilage and Perichondrium
    American Journal of Translational Research, 2016
    Co-Authors: Xiaodie Zhang, Lin Qi, Jia Zhou
    Abstract:

    Auricular cartilage loss or defect remains a challenge to plastic surgeons, and cartilage regenerative medicine provides a novel method to solve the problem. However, ideal seeding cells seem to be the key point in the development of cartilage regeneration. Although bone marrow-mesenchymal stem cells were considered as the ideal seeding cells in cartilage regeneration, regenerative cartilage differentiated from bone marrow-mesenchymal stem cells still faces some problems. It is reported that many tissues and organs contain a certain number of adult progenitor or stem cells that can replace cells that die or restore tissues and organs after injury. Therefore, we tried to use a fibronectin differential adhesion assay to isolate cartilage stem/progenitor cells from auricular cartilage and Perichondrium. Flow cytometric analysis demonstrated the two cell populations expressed mesenchyme stem cell positive surface marker. Meanwhile, the cells differentiate into osteogenic line, chondrogenic line and adipogenic line under different induction conditions. The proliferation of cartilage stem/progenitor cells derived from Perichondrium was higher than cartilage stem/progenitor cells derived from auricular cartilage. In addition, there is a difference on osteogenic differentiation, chondrogenic differentiation and adipogenic differentiation between these two cell populations. In conclusion, auricular cartilage and Perichondrium both contain cartilage stem/progenitor cells, which may provide an ideal seeding cells for cartilage regeneration.

H L Verwoerdverhoef - One of the best experts on this subject based on the ideXlab platform.

  • the dual role of Perichondrium in cartilage wound healing
    Plastic and Reconstructive Surgery, 2002
    Co-Authors: Mark L G Duynstee, H L Verwoerdverhoef, C D A Verwoerd, Gerjo J V M Van Osch
    Abstract:

    Cartilage structures from the head and neck possess a certain but limited capacity to heal after injury. This capacity is accredited to the Perichondrium. In this study, the role of the inner (cambium) and the outer (fibrous) layers of the Perichondrium in cartilage wound healing in vitro is investigated. For the first time, the possibility of selectively removing the outer Perichondrium layer is presented. Using rabbit ears, three different conditions were created: cartilage explants with both Perichondrium layers intact, cartilage explants with only the outer Perichondrium layer dissected, and cartilage explants with both Perichondrium layers removed. The explants were studied after 0, 3, 7,14, and 21 days of in vitro culturing using histochemistry and immunohistochemistry for Ki-67, collagen type II, transforming growth factor beta 1 (TGFβ1), and fibroblast growth factor 2 (FGF2). When both Perichondrium layers were not disturbed, fibrous cells grew over the cut edges of the explants from day 3 of culture on. New cartilage formation was never observed in this condition. When only the outer Perichondrium layer was dissected from the cartilage explants, new cartilage formation was observed around the whole explant at day 21. When both Perichondrium layers were removed, no alterations were observed at the wound surfaces. The growth factors TGFβ1 and FGF2 were expressed in the entire Perichondrium immediately after explantation. The expression gradually decreased with time in culture. However, the expression of TGFβ1 remained high in the outer Perichondrium layer and the layer of cells growing over the explant. This indicates a role for TGFβ1 in the enhancement of fibrous overgrowth during the cartilage wound-healing process. The results of this experimental in vitro study demonstrate the dual role of Perichondrium in cartilage wound healing. On the one hand, the inner layer of the Perichondrium, adjacent to the cartilage, provides (in time) cells for new cartilage formation. On the other hand, the outer layer rapidly produces fibrous overgrowth, preventing the good cartilage-to-cartilage connection necessary to restore the mechanical function of the structure.

  • chondrogenic potential of in vitro multiplied rabbit Perichondrium cells cultured in alginate beads in defined medium
    Tissue Engineering, 2000
    Co-Authors: Gerjo J V M Van Osch, Simone W Van Der Veen, Elisabeth H Burger, H L Verwoerdverhoef
    Abstract:

    Perichondrium has a chondrogenic capacity and is therefore a candidate tissue for engineering of cartilage in vitro. Donor age and culture conditions probably influence chondrogenesis. The aim of this study was to compare the chondrogenic capacity of ear and nasal Perichondrium from young and adult rabbits, using serum containing and serum-free culture conditions. This study demonstrates that more than 1 million cells can be generated out of 1 cm2 of Perichondrium tissue in 3-5 weeks of culture, irrespective of age. Culturing of these cells in alginate in medium with 2, 10, or 20% fetal calf serum did result in the production of small amounts of glycosaminoglycan, but no collagen type II was demonstrated. When serum was replaced however by insulin-like growth factor-1 (IGF-1) (10 ng/mL) plus transforming growth factor-β2 (TGF-β2) (10 ng/mL) an increased glycosaminoglycan production and induction of collagen type II was found, especially in cells isolated from Perichondrium of the ear. Cells derived from p...

  • the role of trabecular demineralized bone in combination with Perichondrium in the generation of cartilage grafts
    Biomaterials, 1999
    Co-Authors: Gerjo J V M Van Osch, Paul Koppel, Simone W Van Der Veen, Pawel Poppe, Elisabeth H Burger, H L Verwoerdverhoef
    Abstract:

    The use of a composite graft of bovine trabecular demineralized bone matrix (DBM) and Perichondrium has been found a reliable method for in vivo generation of cartilage. In the present study, the mechanism whereby this commercially available matrix increases cartilage formation was investigated. First, the time course of cartilage formation in vivo, in the combined implant of Perichondrium and DBM in the rabbit ear was studied, with special focus on tissue reactions to DBM. DBM was colonized by macrophages from day 3 post-operatively, reaching a maximum after 2 weeks. Only a minimal number of neutrophils was found. After 3 weeks the DBM appeared to be resorbed. In the first week the DBM was invaded with chondroblasts, and chondrogenesis occurred between the first and second week of implantation. After 3 weeks, the initially formed islets of cartilage had fused. Next, the chondrogenic capacity of DBM itself was investigated by implantation of DBM without Perichondrium. This never resulted in cartilage formation. Immunohistochemistry showed only a faint staining of the DBM for growth factors. This indicates a minimal chondrogenic e⁄ect of DBM alone and the requirement of Perichondrium as cell provider. In order to define the conditions which cause chondrogenesis in composites of Perichondrium and DBM, a series of in vitro culture experiments was performed in which the in vivo situation was mimicked step by step. The basic condition was Perichondrium cultured in medium with 10% FCS. In this condition, cartilage formation was variable. Because in the in vivo situation both DBM and macrophages can release growth factors, the e⁄ect of IGF1, TGFb2 or OP1 added to the culture medium was tested. Neither the incidence nor the amount of cartilage formation was stimulated by addition of growth factors. Perichondrium wrapped around DBM in vitro gave cartilage formation in the Perichondrium but the incidence and amount were not significantly stimulated compared to cultures of Perichondrium without DBM. However, cartilage-like cells were found in the DBM suggesting an e⁄ect of DBM on Perichondrium-derived cells. Finally, macrophages and/or blood were added to the composite DBM-Perichondrium to mimic the in vivo situation as close as possible. However, no e⁄ect of this treatment was found. In conclusion, this study indicates that DBM itself has few chondrogenic qualities but functions merely as a spacer for cell ingrowth. The fast resorption of DBM by macrophages in vivo seems of importance for the cartilage forming process, but in vitro the presence of macrophages (in combination with blood) could not enhance chondrogenesis. ( 1999 Elsevier Science Ltd. All rights reserved

  • wound healing of the nasal septal Perichondrium in young rabbits
    Operations Research Letters, 1990
    Co-Authors: C D A Verwoerd, H L Verwoerdverhoef, C A Meeuwis, R O V D Heul
    Abstract:

    The effects of elevation of the Perichondrium of the nasal septum, whether or not followed by resection of the cartilage, was histologically studied in young rabbits. The Perichondrium has proved to be a highly reactive tissue. The degree of reaction varied from a transient oedema to the production of large quantities of cells, differentiating into new cartilage. The type of reaction appeared to depend on the conditions at the inner surface of the elevated Perichondrium. The new cartilage differs from the original septal cartilage in morphology and growth potential. The data described above can contribute to the interpretation of previous observations in children.

Jia Zhou - One of the best experts on this subject based on the ideXlab platform.

  • isolation identification and comparison of cartilage stem progenitor cells from auricular cartilage and Perichondrium
    American Journal of Translational Research, 2016
    Co-Authors: Xiaodie Zhang, Lin Qi, Jia Zhou
    Abstract:

    Auricular cartilage loss or defect remains a challenge to plastic surgeons, and cartilage regenerative medicine provides a novel method to solve the problem. However, ideal seeding cells seem to be the key point in the development of cartilage regeneration. Although bone marrow-mesenchymal stem cells were considered as the ideal seeding cells in cartilage regeneration, regenerative cartilage differentiated from bone marrow-mesenchymal stem cells still faces some problems. It is reported that many tissues and organs contain a certain number of adult progenitor or stem cells that can replace cells that die or restore tissues and organs after injury. Therefore, we tried to use a fibronectin differential adhesion assay to isolate cartilage stem/progenitor cells from auricular cartilage and Perichondrium. Flow cytometric analysis demonstrated the two cell populations expressed mesenchyme stem cell positive surface marker. Meanwhile, the cells differentiate into osteogenic line, chondrogenic line and adipogenic line under different induction conditions. The proliferation of cartilage stem/progenitor cells derived from Perichondrium was higher than cartilage stem/progenitor cells derived from auricular cartilage. In addition, there is a difference on osteogenic differentiation, chondrogenic differentiation and adipogenic differentiation between these two cell populations. In conclusion, auricular cartilage and Perichondrium both contain cartilage stem/progenitor cells, which may provide an ideal seeding cells for cartilage regeneration.

Toshio Suda - One of the best experts on this subject based on the ideXlab platform.

  • mesenchymal stem cells in Perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation
    Journal of Experimental Medicine, 2002
    Co-Authors: Fumio Arai, Osamu Ohneda, Takeshi Miyamoto, Xiuqin Zhang, Toshio Suda
    Abstract:

    Perichondrium in fetal limb is composed of undifferentiated mesenchymal cells. However, the multipotency of cells in this region and the role of Perichondrium in bone marrow formation are not well understood. In this report, we purified and characterized perichondrial cells using a monoclonal antibody against activated leukocyte cell adhesion molecule (ALCAM) and investigated the role of perichondrial cells in hematopoietic bone marrow formation. ALCAM is expressed on hematopoietic cells, endothelial cells, bone marrow stromal cells, and mesenchymal stem cells and mediates homophilic (ALCAM–ALCAM)/heterophilic (ALCAM-CD6) cell adhesion. Here we show by immunohistochemical staining that ALCAM is expressed in Perichondrium. ALCAM+ perichondrial cells isolated by FACS® exhibit the characteristics of mesenchymal stem cells. ALCAM+ cells can differentiate into osteoblasts, adipocytes, chondrocytes, and stromal cells, which can support osteoclastogenesis, hematopoiesis, and angiogenesis. Furthermore, the addition of ALCAM-Fc or CD6-Fc to the metatarsal culture, the invasion of the blood vessels to a cartilage was inhibited. Our findings indicate that ALCAM+ perichondrial cells participate in vascular invasion by recruiting osteoclasts and vessels. These findings suggest that Perichondrium might serve as a stem cell reservoir and play an important role in the early development of a bone and bone marrow.

Zhuxin Chen - One of the best experts on this subject based on the ideXlab platform.

  • the in vivo chondrogenesis of cartilage stem progenitor cells from auricular cartilage and the Perichondrium
    American Journal of Translational Research, 2019
    Co-Authors: Xiaodie Zhang, Lin Qi, Yahong Chen, Zhezheng Xiong, Junjie Li, Peng Xu, Huizhong Zhang, Zhuxin Chen
    Abstract:

    Bone marrow-derived stem cells are commonly studied for cartilage tissue engineering and regeneration medicine applications, but their ossification tendency and their limited capacity for chondrogenic differentiation depending on the donor age limit their clinical application. Cartilage stem/progenitor cells are ideal seeding cells, as cartilage stem/progenitor cells from auricular cartilage and the Perichondrium have the inherent advantages of chondrogenesis capacity and an easy and nontraumatic harvesting process, displaying promise for applications. The identification and comparison of cartilage stem/progenitor cells from auricular cartilage and the Perichondrium in vitro were explored in our previous study, but the in vivo chondrogenesis of these cells has not been fully examined. In the current study, we explored the ectopic chondrogenesis of cartilage stem progenitor/cells from auricular cartilage and the Perichondrium after chondrogenic induction in vitro. Our results suggest that stem/progenitor cells from auricular cartilage exhibit significantly better chondrogenesis than those from the Perichondrium in vivo, with upregulated chondrogenic genes and a stable cartilage phenotype, as well as good mechanical properties, indicating that stem/progenitor cells from auricular cartilage could be one type of ideal seeding cells for cartilage tissue engineering.

  • The in vivo chondrogenesis of cartilage stem/progenitor cells from auricular cartilage and the Perichondrium.
    American Journal of Translational Research, 2019
    Co-Authors: Xiaodie Zhang, Lin Qi, Yahong Chen, Zhezheng Xiong, Junjie Li, Peng Xu, Huizhong Zhang, Zhuxin Chen
    Abstract:

    Bone marrow-derived stem cells are commonly studied for cartilage tissue engineering and regeneration medicine applications, but their ossification tendency and their limited capacity for chondrogenic differentiation depending on the donor age limit their clinical application. Cartilage stem/progenitor cells are ideal seeding cells, as cartilage stem/progenitor cells from auricular cartilage and the Perichondrium have the inherent advantages of chondrogenesis capacity and an easy and nontraumatic harvesting process, displaying promise for applications. The identification and comparison of cartilage stem/progenitor cells from auricular cartilage and the Perichondrium in vitro were explored in our previous study, but the in vivo chondrogenesis of these cells has not been fully examined. In the current study, we explored the ectopic chondrogenesis of cartilage stem progenitor/cells from auricular cartilage and the Perichondrium after chondrogenic induction in vitro. Our results suggest that stem/progenitor cells from auricular cartilage exhibit significantly better chondrogenesis than those from the Perichondrium in vivo, with upregulated chondrogenic genes and a stable cartilage phenotype, as well as good mechanical properties, indicating that stem/progenitor cells from auricular cartilage could be one type of ideal seeding cells for cartilage tissue engineering.