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B Koudela - One of the best experts on this subject based on the ideXlab platform.

  • Republic
    2016
    Co-Authors: Jiří Volf, David Modrý, B Koudela
    Abstract:

    Abstract. Four laboratory-hatched European kestrels Falco tinnunculus L. were fed on laboratory mice and common voles Microtus arvalis Pallas previously inoculated with different doses of sporulated oocysts of Caryospora kutzeri Böer, 1982. Two kestrels that were fed infected mice shed C. kutzeri oocysts 6 days after ingesting murine tissues. To compare direct and indirect transmissions, two of the kestrels were subsequently directly inoculated with 105 sporulated C. kutzeri oocysts and became patent on days 8 and 9 and shed Caryosporan oocysts up to day 25 post inoculation. Additionally, four mice were inoculated with 106 oocysts in order to examine mouse tissues for the presence of developmental stages of C. kutzeri. No coccidian stages were found in the tissues of inoculated mice. The experiment showed that developmental stages of C. kutzeri are able to survive in mouse tissues and cause infection of suitable host after their ingestion. The coccidian genus Caryospora Léger, 1904, the third largest genus within the family Eimeriidae represents especially parasites of snakes and raptorial birds. The members of this genus were considered monoxenous up to the early eighties. The experimental study of the life cycle of Caryospora bubonis Stockdale et Cawthorn, 1981 suggested that some Caryospora

  • n. sp., a new coccidian parasite (Apicomplexa: Eimeriidae) from the horned bush-viper,
    EDP Sciences, 2014
    Co-Authors: David Modrý, Jan Šlapeta, B Koudela
    Abstract:

    Caryospora matatu n. sp. is the first species of coccidia reported from the a horned bush viper, Atheris ceralophorus endemic to Tanzania. Oocysts are spherical or slightly subspherical, 19.8 (16-23) µm, a micropyle and an oocyst residuum are absent, a single polar granule is present. An oocyst wall is bilayered, ~ 1.5 µm thick, brownish and distinctly pitted. Sporocysts are ellipsoidal, 15.6 (12.5-17.0) x 10.1 (8-12) µm, tightly fitting an internal oocyst diameter, Stieda and substieda bodies are present. An experimental inoculation of SCID mice did not confirm a facultatively heteroxenous life cycle in the studied coccidium. Morphology and evolutionary history of Caryospora species from viperids is is reviewed and discussed

  • Caryospora KALIMANTANENSIS SP. N. (APICOMPLEXA: EIMERIIDAE) FROM THE MANGROVE SNAKE BOIGA DENDROPHILA (SERPENTES: BOIGINAE) FROM KALIMANTAN
    Folia Parasitologica, 2013
    Co-Authors: David Modrý, B Koudela
    Abstract:

    A new coccidian parasite of the genus Caryospora Leger is described from the colubrid snake Boiga dendrophila Boie, 1827 imported from Kalimantan. Oocysts of Caryospora kalimantanensis sp. n. are spherical, 18.7 (17.0-20.0) mu m in diameter, with a bilayered slightly pitted and brownish oocyst wall. A micropyle and oocyst residuum are absent. One globular polar granule is present in 45% of sporulated oocysts. Sporocysts are ovoidal to ellipsoidal, 14.5 (13.0-15.5) x 10.5 (10.0-11.0) mu m, with prominent Stieda and substieda bodies. Sporocyst residuum is present as small granules scattered among sporozoites. Sporozoites are elongate, lying lengthwise and parallel within the sporocyst and completely filling the sporocyst. Each sporozoite contains a spherical anterior and posterior refractile bodies.

  • Mice serve as paratenic hosts for the transmission of Caryospora duszynskii (Apicomplexa: Eimeriidae) between snakes of the genus Elaphe.
    Folia parasitologica, 2005
    Co-Authors: David Modrý, Jan Šlapeta, B Koudela
    Abstract:

    Caryospora duszynskii Upton, Current et Barnard, 1984 was successfully transmitted to snakes of the genus Elaphe by feeding them previously infected mice. Fifty thousand oocysts were orally administered to two mouse strains, BALB/c and Crl:CD-1(ICR)BR, which were subsequently fed to captive-born coccidia-free Elaphe guttata (L.) in two respective independent experiments. Both E. guttata expelled C. duszynskii oocysts in their faeces, beginning on day 18 and 26 post infection (p.i.) and shed oocysts continuously through the end of the experiment, day 230 and 135 p.i., respectively. There were no parasitic stages or lesions in mice, as revealed by histological examination. Experiments proved that rodents serve as paratenic hosts for C. duszynskii. In summary we discuss the life-cycle strategies of Caryospora spp. in reptiles and present three general modes of their development.

  • Caryospora matatu n. sp., a new coccidian parasite (Apicomplexa: Eimeriidae) from the horned bush-viper, Atheris ceratophorus Werner, 1895 from Tanzania.
    Parasite (Paris France), 2002
    Co-Authors: David Modrý, Jan Šlapeta, B Koudela
    Abstract:

    Caryospora matatu n. sp. is the first species of coccidia reported from the a horned bush viper, Atheris ceratophorus endemic to Tanzania. Oocysts are spherical or slightly subspherical, 19.8 (16-23) microns, a micropyle and an oocyst residuum are absent, a single polar granule is present. An oocyst wall is bilayered, approximately 1.5 microns thick, brownish and distinctly pitted. Sporocysts are ellipsoidal, 15.6 (12.5-17.0) x 10.1 (8-12) microns, tightly fitting an internal oocyst diameter, Stieda and substieda bodies are present. An experimental inoculation of SCID mice did not confirm a facultatively heteroxenous life cycle in the studied coccidium. Morphology and evolutionary history of Caryospora species from viperids is is reviewed and discussed.

David Modrý - One of the best experts on this subject based on the ideXlab platform.

  • Republic
    2016
    Co-Authors: Jiří Volf, David Modrý, B Koudela
    Abstract:

    Abstract. Four laboratory-hatched European kestrels Falco tinnunculus L. were fed on laboratory mice and common voles Microtus arvalis Pallas previously inoculated with different doses of sporulated oocysts of Caryospora kutzeri Böer, 1982. Two kestrels that were fed infected mice shed C. kutzeri oocysts 6 days after ingesting murine tissues. To compare direct and indirect transmissions, two of the kestrels were subsequently directly inoculated with 105 sporulated C. kutzeri oocysts and became patent on days 8 and 9 and shed Caryosporan oocysts up to day 25 post inoculation. Additionally, four mice were inoculated with 106 oocysts in order to examine mouse tissues for the presence of developmental stages of C. kutzeri. No coccidian stages were found in the tissues of inoculated mice. The experiment showed that developmental stages of C. kutzeri are able to survive in mouse tissues and cause infection of suitable host after their ingestion. The coccidian genus Caryospora Léger, 1904, the third largest genus within the family Eimeriidae represents especially parasites of snakes and raptorial birds. The members of this genus were considered monoxenous up to the early eighties. The experimental study of the life cycle of Caryospora bubonis Stockdale et Cawthorn, 1981 suggested that some Caryospora

  • n. sp., a new coccidian parasite (Apicomplexa: Eimeriidae) from the horned bush-viper,
    EDP Sciences, 2014
    Co-Authors: David Modrý, Jan Šlapeta, B Koudela
    Abstract:

    Caryospora matatu n. sp. is the first species of coccidia reported from the a horned bush viper, Atheris ceralophorus endemic to Tanzania. Oocysts are spherical or slightly subspherical, 19.8 (16-23) µm, a micropyle and an oocyst residuum are absent, a single polar granule is present. An oocyst wall is bilayered, ~ 1.5 µm thick, brownish and distinctly pitted. Sporocysts are ellipsoidal, 15.6 (12.5-17.0) x 10.1 (8-12) µm, tightly fitting an internal oocyst diameter, Stieda and substieda bodies are present. An experimental inoculation of SCID mice did not confirm a facultatively heteroxenous life cycle in the studied coccidium. Morphology and evolutionary history of Caryospora species from viperids is is reviewed and discussed

  • Caryospora KALIMANTANENSIS SP. N. (APICOMPLEXA: EIMERIIDAE) FROM THE MANGROVE SNAKE BOIGA DENDROPHILA (SERPENTES: BOIGINAE) FROM KALIMANTAN
    Folia Parasitologica, 2013
    Co-Authors: David Modrý, B Koudela
    Abstract:

    A new coccidian parasite of the genus Caryospora Leger is described from the colubrid snake Boiga dendrophila Boie, 1827 imported from Kalimantan. Oocysts of Caryospora kalimantanensis sp. n. are spherical, 18.7 (17.0-20.0) mu m in diameter, with a bilayered slightly pitted and brownish oocyst wall. A micropyle and oocyst residuum are absent. One globular polar granule is present in 45% of sporulated oocysts. Sporocysts are ovoidal to ellipsoidal, 14.5 (13.0-15.5) x 10.5 (10.0-11.0) mu m, with prominent Stieda and substieda bodies. Sporocyst residuum is present as small granules scattered among sporozoites. Sporozoites are elongate, lying lengthwise and parallel within the sporocyst and completely filling the sporocyst. Each sporozoite contains a spherical anterior and posterior refractile bodies.

  • Mice serve as paratenic hosts for the transmission of Caryospora duszynskii (Apicomplexa: Eimeriidae) between snakes of the genus Elaphe.
    Folia parasitologica, 2005
    Co-Authors: David Modrý, Jan Šlapeta, B Koudela
    Abstract:

    Caryospora duszynskii Upton, Current et Barnard, 1984 was successfully transmitted to snakes of the genus Elaphe by feeding them previously infected mice. Fifty thousand oocysts were orally administered to two mouse strains, BALB/c and Crl:CD-1(ICR)BR, which were subsequently fed to captive-born coccidia-free Elaphe guttata (L.) in two respective independent experiments. Both E. guttata expelled C. duszynskii oocysts in their faeces, beginning on day 18 and 26 post infection (p.i.) and shed oocysts continuously through the end of the experiment, day 230 and 135 p.i., respectively. There were no parasitic stages or lesions in mice, as revealed by histological examination. Experiments proved that rodents serve as paratenic hosts for C. duszynskii. In summary we discuss the life-cycle strategies of Caryospora spp. in reptiles and present three general modes of their development.

  • Caryospora matatu n. sp., a new coccidian parasite (Apicomplexa: Eimeriidae) from the horned bush-viper, Atheris ceratophorus Werner, 1895 from Tanzania.
    Parasite (Paris France), 2002
    Co-Authors: David Modrý, Jan Šlapeta, B Koudela
    Abstract:

    Caryospora matatu n. sp. is the first species of coccidia reported from the a horned bush viper, Atheris ceratophorus endemic to Tanzania. Oocysts are spherical or slightly subspherical, 19.8 (16-23) microns, a micropyle and an oocyst residuum are absent, a single polar granule is present. An oocyst wall is bilayered, approximately 1.5 microns thick, brownish and distinctly pitted. Sporocysts are ellipsoidal, 15.6 (12.5-17.0) x 10.1 (8-12) microns, tightly fitting an internal oocyst diameter, Stieda and substieda bodies are present. An experimental inoculation of SCID mice did not confirm a facultatively heteroxenous life cycle in the studied coccidium. Morphology and evolutionary history of Caryospora species from viperids is is reviewed and discussed.

Tsutomu Morii - One of the best experts on this subject based on the ideXlab platform.

  • infectivity and sporogony of Caryospora type oocysts of isospora rivolta obtained by heating
    Parasitology Research, 1993
    Co-Authors: Toshihiro Matsui, Singo Ito, Takashi Fujino, Tsutomu Morii
    Abstract:

    The infectivity and sporogony of Caryospora-type oocysts of Isospora rivolta obtained by heat treatment were examined. Fresh unsporulated oocysts of I. rivolta were heated at 50 degrees C for 5 min, then poured into a cold 2% potassium dichromate solution and incubated at 25 degrees C. To observe the stage of sporulation, 100 oocysts were examined at 2-h intervals after incubation. The earliest sporulated oocysts were observed at 14 h and the sporulation time was 22 h, similar to that of nontreated oocysts. To determine the infectivity, only Caryospora-type oocysts were collected after sporulation and inoculated orally into cats and mice. The mice were killed at 7 days after inoculation, and their mesenteric lymph nodes and spleens were fed to other cats. All of these cats shed oocysts. The newly discharged oocysts developed into the Isospora type after sporulation. These results suggest that the Caryospora-type oocysts transformed only morphologically after heat treatment and were as infective as the nontreated oocysts to host animals.

  • Infectivity and sporogony of Caryospora-type oocysts of Isospora rivolta obtained by heating.
    Parasitology Research, 1993
    Co-Authors: Toshihiro Matsui, Singo Ito, Takashi Fujino, Tsutomu Morii
    Abstract:

    The infectivity and sporogony ofCaryospora-type oocysts ofIsospora rivolta obtained by heat treatment were examined. Fresh unsporulated oocysts ofI. rivolta were heated at 50°C for 5 min, then poured into a cold 2% potassium dichromate solution and incubated at 25°C. To observe the stage of sporulation, 100 oocysts were examined at 2-h intervals after incubation. The earliest sporulated oocysts were observed at 14 h and the sporulation time was 22 h, similar to that of nontreated oocysts. To determine the infectivity, onlyCaryospora-type oocysts were collected after sporulation and inoculated orally into cats and mice. The mice were killed at 7 days after inoculation, and their mesenteric lymph nodes and spleens were fed to other cats. All of these cats shed oocysts. The newly discharged oocysts developed into theIsospora type after sporulation. These results suggest that theCaryospora-type oocysts transformed only morphologically after heat treatment and were as infective as the nontreated oocysts to host antimals.

Jan Šlapeta - One of the best experts on this subject based on the ideXlab platform.

  • n. sp., a new coccidian parasite (Apicomplexa: Eimeriidae) from the horned bush-viper,
    EDP Sciences, 2014
    Co-Authors: David Modrý, Jan Šlapeta, B Koudela
    Abstract:

    Caryospora matatu n. sp. is the first species of coccidia reported from the a horned bush viper, Atheris ceralophorus endemic to Tanzania. Oocysts are spherical or slightly subspherical, 19.8 (16-23) µm, a micropyle and an oocyst residuum are absent, a single polar granule is present. An oocyst wall is bilayered, ~ 1.5 µm thick, brownish and distinctly pitted. Sporocysts are ellipsoidal, 15.6 (12.5-17.0) x 10.1 (8-12) µm, tightly fitting an internal oocyst diameter, Stieda and substieda bodies are present. An experimental inoculation of SCID mice did not confirm a facultatively heteroxenous life cycle in the studied coccidium. Morphology and evolutionary history of Caryospora species from viperids is is reviewed and discussed

  • Mice serve as paratenic hosts for the transmission of Caryospora duszynskii (Apicomplexa: Eimeriidae) between snakes of the genus Elaphe.
    Folia parasitologica, 2005
    Co-Authors: David Modrý, Jan Šlapeta, B Koudela
    Abstract:

    Caryospora duszynskii Upton, Current et Barnard, 1984 was successfully transmitted to snakes of the genus Elaphe by feeding them previously infected mice. Fifty thousand oocysts were orally administered to two mouse strains, BALB/c and Crl:CD-1(ICR)BR, which were subsequently fed to captive-born coccidia-free Elaphe guttata (L.) in two respective independent experiments. Both E. guttata expelled C. duszynskii oocysts in their faeces, beginning on day 18 and 26 post infection (p.i.) and shed oocysts continuously through the end of the experiment, day 230 and 135 p.i., respectively. There were no parasitic stages or lesions in mice, as revealed by histological examination. Experiments proved that rodents serve as paratenic hosts for C. duszynskii. In summary we discuss the life-cycle strategies of Caryospora spp. in reptiles and present three general modes of their development.

  • Caryospora matatu n. sp., a new coccidian parasite (Apicomplexa: Eimeriidae) from the horned bush-viper, Atheris ceratophorus Werner, 1895 from Tanzania.
    Parasite (Paris France), 2002
    Co-Authors: David Modrý, Jan Šlapeta, B Koudela
    Abstract:

    Caryospora matatu n. sp. is the first species of coccidia reported from the a horned bush viper, Atheris ceratophorus endemic to Tanzania. Oocysts are spherical or slightly subspherical, 19.8 (16-23) microns, a micropyle and an oocyst residuum are absent, a single polar granule is present. An oocyst wall is bilayered, approximately 1.5 microns thick, brownish and distinctly pitted. Sporocysts are ellipsoidal, 15.6 (12.5-17.0) x 10.1 (8-12) microns, tightly fitting an internal oocyst diameter, Stieda and substieda bodies are present. An experimental inoculation of SCID mice did not confirm a facultatively heteroxenous life cycle in the studied coccidium. Morphology and evolutionary history of Caryospora species from viperids is is reviewed and discussed.

  • Caryospora varaniornati sp. n. (Apicomplexa: Eimeriidae) in the Nile monitor, Varanus (Polydaedalus) niloticus species complex.
    Folia parasitologica, 2001
    Co-Authors: David Modrý, Jan Šlapeta, Zdeněk Knotek, B Koudela
    Abstract:

    Parasitological examination of two ornate Nile monitors Varanus ornatus (Daudin, 1803) imported from Benin revealed the presence of a new species of Caryospora. Oocysts of Caryospora varaniornati sp. n. are spherical to slightly subspherical, 12.0 (11-12.5) × 11.5 (11-12) µm, without a micropyle and oocyst residuum, and occasionally possessing one small polar granule. Sporocysts are broadly ellipsoidal, 8.8 (8.5-9.5) × 6.7 (6.5-7) µm; a lentil-like Stieda body is present, ca. 0.5 × 1 µm; substieda body not visible. Experimental infection of a closely related host, Varanus niloticus (L.), did not lead to the oocyst excretion despite the fact that one of the experimentally inoculated monitors was immunosuppressed by dexamethasone. Histological examination did not reveal stages of coccidian development. Therefore, it is possible that C. varaniornati is strictly host specific.

  • SCID mice as a tool for evaluation of heteroxenous life cycle pattern of Caryospora (Apicomplexa, Eimeriidae) species
    Veterinary Parasitology, 2000
    Co-Authors: B Koudela, David Modrý, Jiří Volf, Jan Šlapeta
    Abstract:

    Adult severe combined immunodeficient (SCID) mice were inoculated with oocysts of 13 different Caryospora (Protozoa, Apicomplexa) species isolated from the faeces of 10 reptilian and three raptorial bird hosts in attempt to test heteroxenous life cycle pattern. Only three reptilian isolates originated from viperid snakes, namely from Calloselasma rhodostoma, Atheris nitschei and Vipera ursinii induced lethal dermal caryosporosis in SCID mice. Neither clinical signs nor developmental stages were observed in mice infected with further nine Caryosporan isolates originated from other reptilian and raptorial bird hosts. Results of this study confirmed that SCID mice represent a useful tool for evaluation of heteroxenous life cycle pattern of Caryosporan coccidia and that only the Caryospora species from viperid and crotalid snakes produce dermal caryosporosis in mice

Donald W. Duszynski - One of the best experts on this subject based on the ideXlab platform.