The Experts below are selected from a list of 273 Experts worldwide ranked by ideXlab platform
Didier Bogusz - One of the best experts on this subject based on the ideXlab platform.
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Plant and Soil 254: 229–237, 2003. © 2003 Kluwer Academic Publishers. Printed in the Netherlands.
2013Co-Authors: Carole Santi, Didier Bogusz, Florence Auguy, Emile Duhoux, Sergio Svistoonoff, Laure Constans, Claudine FrancheAbstract:Choosing a reporter for gene expression studies in transgenic actinorhizal plants of the Casuarinaceae famil
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Auxin Influx Activity Is Associated with Frankia Infection during Actinorhizal Nodule Formation in Casuarina glauca
PLANT PHYSIOLOGY, 2007Co-Authors: Benjamin Peret, Gaetan Devos, Claudine Franche, Carole Santi, Valérie Hocher, Florence Auguy, Ranjan Swarup, L Jansen, Matthew Collin, Didier BoguszAbstract:Plants from the Casuarinaceae family enter symbiosis with the actinomycete Frankia leading to the formation of nitrogen-fixing root nodules. We observed that application of the auxin influx inhibitor 1-naphtoxyacetic acid perturbs actinorhizal nodule formation. This suggests a potential role for auxin influx carriers in the infection process. We therefore isolated and characterized homologs of the auxin influx carrier (AUX1-LAX) genes in Casuarina glauca. Two members of this family were found to share high levels of deduced protein sequence identity with Arabidopsis (Arabidopsis thaliana) AUX-LAX proteins. Complementation of the Arabidopsis aux1 mutant revealed that one of them is functionally equivalent to AUX1 and was named CgAUX1. The spatial and temporal expression pattern of CgAUX1 promoter:beta-glucuronidase reporter was analyzed in Casuarinaceae. We observed that CgAUX1 was expressed in plant cells infected by Frankia throughout the course of actinorhizal nodule formation. Our data suggest that auxin plays an important role during plant cell infection in actinorhizal symbioses.
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Auxin Influx Activity Is Associated with Frankia Infection during Actinorhizal Nodule Formation in
2007Co-Authors: Ranjan Swarup, Claudine Franche, Carole Santi, Didier Bogusz, Florence Auguy, L Jansen, Myriam Collin, Malcolm J. Bennett, Laurent LaplazeAbstract:Plants from the Casuarinaceae family enter symbiosis with the actinomycete Frankia leading to the formation of nitrogen-fixing root nodules. We observed that application of the auxin influx inhibitor 1-naphtoxyacetic acid perturbs actinorhizal nodule formation. This suggests a potential role for auxin influx carriers in the infection process. We therefore isolated and characterized homologs of the auxin influx carrier (AUX1-LAX) genes in Casuarina glauca. Two members of this family were found to share high levels of deduced protein sequence identity with Arabidopsis (Arabidopsis thaliana) AUX-LAX proteins. Complementation of the Arabidopsis aux1 mutant revealed that one of them is functionally equivalent to AUX1 and was named CgAUX1. The spatial and temporal expression pattern of CgAUX1 promoter:b-glucuronidase reporter was analyzed in Casuarinaceae. We observed that CgAUX1 was expressed in plant cells infected by Frankia throughout the course of actinorhizal nodule formation. Our data suggest that auxin plays an important role during plant cell infection in actinorhizal symbioses. Actinorhizal plants, which belong to eight families of angiosperms, can form nitrogen-fixing nodules in symbiosis with the soil actinomycete Frankia (Benson and Silvester, 1993). The symbiotic interaction starts, under conditions of nitrogen deprivation, by an exchange of signals between the plant roots and bacteria. The chemical nature of Frankia nodulation factors is unknown, but data suggest that it has different bio
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cg12 expression is specifically linked to infection of root hairs and cortical cells during casuarina glauca and allocasuarina verticillata actinorhizal nodule development
Molecular Plant-microbe Interactions, 2003Co-Authors: Sergio Svistoonoff, Claudine Franche, Florence Auguy, Laurent Laplaze, John Runions, Robin Duponnois, Jim Haseloff, Didier BoguszAbstract:cg12 is an early actinorhizal nodulin gene from Casuarina glauca encoding a subtilisin-like serine protease. Using transgenic Casuarinaceae plants carrying cg12-gus and cg12-gfp fusions, we have studied the expression pattern conferred by the cg12 promoter region after inoculation with Frankia. cg12 was found to be expressed in root hairs and in root and nodule cortical cells containing Frankia infection threads. cg12 expression was also monitored after inoculation with ineffective Frankia strains, during my-corrhizae formation, and after diverse hormonal treatments. None of these treatments was able to induce its expression, therefore suggesting that cg12 expression is linked to plant cell infection by Frankia strains. Possible roles of cg12 in actinorhizal symbiosis are discussed.
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Choosing a reporter for gene expression studies in transgenic actinorhizal plants of the Casuarinaceae family
Frankia Symbiosis, 2003Co-Authors: Carole Santi, Didier Bogusz, Florence Auguy, Emile Duhoux, Sergio Svistoonoff, Laure Constans, Claudine FrancheAbstract:Transgenic Casuarinaceae and reporter genes provide valuable tools to study gene expression in transgenic actinorhizal nodules. In this paper, we discuss the use of s-glucuronidase for the histochemical localization and quantification of gene expression in transgenic plants of Allocasuarina verticillata and Casuarina glauca nodulated by the actinomycete Frankia. We also report on the genetic transformation of A. verticillata by the Agrobacterium tumefaciens strain C58C1(pGV2260) containing the 35S-mgfp5-ER construct encoding a modified green fluorescent protein of Aequorea victoria in a binary vector. The evolution of the GFP fluorescence was monitored through all stages of the regeneration process. The data indicate that GFP is not toxic in Casuarinaceae and that this reporter gene can be used for visual screening of transformed calli and transgenic plants. The fluorescence pattern of gfp provides a new tool for monitoring in vivo transgenc expression in actinorhizal plants.
Laurent Laplaze - One of the best experts on this subject based on the ideXlab platform.
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Identification of potential transcriptional regulators of actinorhizal symbioses in Casuarina glauca and Alnus glutinosa
BMC Plant Biology, 2014Co-Authors: Hassen Gherbi, Valérie Hocher, Laurent Laplaze, Antony Champion, Diaga Diouf, Issa Diédhiou, Alexandre Tromas, Maïmouna Cissoko, Krystelle Gray, Boris ParizotAbstract:Trees belonging to the Casuarinaceae and Betulaceae families play an important ecological role and are useful tools in forestry for degraded land rehabilitation and reforestation. These functions are linked to their capacity to establish symbiotic relationships with a nitrogen-fixing soil bacterium of the genus Frankia. However, the molecular mechanisms controlling the establishment of these symbioses are poorly understood. The aim of this work was to identify potential transcription factors involved in the establishment and functioning of actinorhizal symbioses.
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Contribution of transgenic Casuarinaceae to our knowledge of the actinorhizal symbioses
Symbiosis, 2010Co-Authors: Sergio Svistoonoff, Valérie Hocher, Florence Auguy, Laurent Laplaze, Hassen Gherbi, Mathish Nambiar-veetil, Chonglu Zhong, Zuzanna Michalak, Virginie Vaissayre, Patrick DoumasAbstract:The Casuarinaceae family is a group of 96 species of trees and shrubs that are tolerant to adverse soil and climatic conditions. In the field, Casuarinaceae bears nitrogen-fixing root nodules (so called actinorhizal nodules) resulting from infection by the soil actinomycete Frankia. The association between Casuarina and Frankia is of tremendous ecological importance in tropical and subtropical areas where these trees contribute to land stabilization and soil reclamation. During differentiation of the actinorhizal nodule, a set of genes called actinorhizal nodulins is activated in the developing nodule. Understanding the molecular basis of actinorhizal nodule ontogenesis requires molecular tools such as genomics together with gene transfer technologies for functional analysis of symbiotic genes. Using the biological vectors Agrobacterium rhizogenes and A. tumefaciens, gene transfer into the two species Allocasuarina verticillata and Casuarina glauca has been successful. Transgenic Casuarinaceae plants proved to be valuable tools for exploring the molecular mechanisms resulting from the infection process of actinorhizal plants by Frankia.
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Auxin Influx Activity Is Associated with Frankia Infection during Actinorhizal Nodule Formation in
2007Co-Authors: Ranjan Swarup, Claudine Franche, Carole Santi, Didier Bogusz, Florence Auguy, L Jansen, Myriam Collin, Malcolm J. Bennett, Laurent LaplazeAbstract:Plants from the Casuarinaceae family enter symbiosis with the actinomycete Frankia leading to the formation of nitrogen-fixing root nodules. We observed that application of the auxin influx inhibitor 1-naphtoxyacetic acid perturbs actinorhizal nodule formation. This suggests a potential role for auxin influx carriers in the infection process. We therefore isolated and characterized homologs of the auxin influx carrier (AUX1-LAX) genes in Casuarina glauca. Two members of this family were found to share high levels of deduced protein sequence identity with Arabidopsis (Arabidopsis thaliana) AUX-LAX proteins. Complementation of the Arabidopsis aux1 mutant revealed that one of them is functionally equivalent to AUX1 and was named CgAUX1. The spatial and temporal expression pattern of CgAUX1 promoter:b-glucuronidase reporter was analyzed in Casuarinaceae. We observed that CgAUX1 was expressed in plant cells infected by Frankia throughout the course of actinorhizal nodule formation. Our data suggest that auxin plays an important role during plant cell infection in actinorhizal symbioses. Actinorhizal plants, which belong to eight families of angiosperms, can form nitrogen-fixing nodules in symbiosis with the soil actinomycete Frankia (Benson and Silvester, 1993). The symbiotic interaction starts, under conditions of nitrogen deprivation, by an exchange of signals between the plant roots and bacteria. The chemical nature of Frankia nodulation factors is unknown, but data suggest that it has different bio
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cg12 expression is specifically linked to infection of root hairs and cortical cells during casuarina glauca and allocasuarina verticillata actinorhizal nodule development
Molecular Plant-microbe Interactions, 2003Co-Authors: Sergio Svistoonoff, Claudine Franche, Florence Auguy, Laurent Laplaze, John Runions, Robin Duponnois, Jim Haseloff, Didier BoguszAbstract:cg12 is an early actinorhizal nodulin gene from Casuarina glauca encoding a subtilisin-like serine protease. Using transgenic Casuarinaceae plants carrying cg12-gus and cg12-gfp fusions, we have studied the expression pattern conferred by the cg12 promoter region after inoculation with Frankia. cg12 was found to be expressed in root hairs and in root and nodule cortical cells containing Frankia infection threads. cg12 expression was also monitored after inoculation with ineffective Frankia strains, during my-corrhizae formation, and after diverse hormonal treatments. None of these treatments was able to induce its expression, therefore suggesting that cg12 expression is linked to plant cell infection by Frankia strains. Possible roles of cg12 in actinorhizal symbiosis are discussed.
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symbiotic and non symbiotic expression of cgmt1 a metallothionein like gene from the actinorhizal tree casuarina glauca
Plant Molecular Biology, 2002Co-Authors: Laurent Laplaze, Claudine Franche, Florence Auguy, Katharina Pawlowski, Emile Duhoux, H. Gherbi, Fathia Guermache, Didier BoguszAbstract:A clone for a type 1 metallothionein (cgMT1) was isolated from a Casuarina glauca nodule cDNA library. The corresponding gene belongs to a small family and is highly expressed in roots and nitrogen-fixing nodules, whereas low expression was observed in aerial parts of the plant. The promoter region of cgMT1 was isolated and fused to the β-glucuronidase (gus) gene. Transgenic Casuarinaceae plants showed that the cgMT1 promoter was most active in roots and in the oldest region of the shoot. In situ hybridization indicated that in nodules cgMT1 transcript is present in mature Frankia-infected cells and in the pericycle. Possible roles for cgMT1 in symbiotic and non-symbiotic tissues are discussed.
Claudine Franche - One of the best experts on this subject based on the ideXlab platform.
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Plant and Soil 254: 229–237, 2003. © 2003 Kluwer Academic Publishers. Printed in the Netherlands.
2013Co-Authors: Carole Santi, Didier Bogusz, Florence Auguy, Emile Duhoux, Sergio Svistoonoff, Laure Constans, Claudine FrancheAbstract:Choosing a reporter for gene expression studies in transgenic actinorhizal plants of the Casuarinaceae famil
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Potential Casuarina species and suitable techniques for the GGW
Le projet majeur africain de la Grande Muraille Verte, 2010Co-Authors: Zhong Chonglu, Zhang Yong, Chen Zhen, Jiang Qingbin, Khongsak Pinyopusarerk, Claudine FrancheAbstract:Casuarina tree species belong to the Casuarinaceae family, which includes 4 genera and 96 species. Casuarina trees are native fromAustralia, Southeast Asia and Pacific archipelagoes. Casuarinas develop symbiotic associations with Frankia, ectomycorrhizal and endomycorrhizal fungi. They have been introduced into tropical and subtropical zones in the world. Casuarinas are economically and ecologically important. They are multipurpose tree species providing a wide range of goods and services, such as windbreaks, sand stabilization, agroforestry and general rehabilitation for difficult sites. Casuarinas can be planted as pioneer species in hot-dry river valley, dry sandy soil, rock mountain and around desert. The wood is a main source of fuelwood and charcoal, for general construction, fiber-wood, and other wood-based industries.
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Auxin Influx Activity Is Associated with Frankia Infection during Actinorhizal Nodule Formation in Casuarina glauca
PLANT PHYSIOLOGY, 2007Co-Authors: Benjamin Peret, Gaetan Devos, Claudine Franche, Carole Santi, Valérie Hocher, Florence Auguy, Ranjan Swarup, L Jansen, Matthew Collin, Didier BoguszAbstract:Plants from the Casuarinaceae family enter symbiosis with the actinomycete Frankia leading to the formation of nitrogen-fixing root nodules. We observed that application of the auxin influx inhibitor 1-naphtoxyacetic acid perturbs actinorhizal nodule formation. This suggests a potential role for auxin influx carriers in the infection process. We therefore isolated and characterized homologs of the auxin influx carrier (AUX1-LAX) genes in Casuarina glauca. Two members of this family were found to share high levels of deduced protein sequence identity with Arabidopsis (Arabidopsis thaliana) AUX-LAX proteins. Complementation of the Arabidopsis aux1 mutant revealed that one of them is functionally equivalent to AUX1 and was named CgAUX1. The spatial and temporal expression pattern of CgAUX1 promoter:beta-glucuronidase reporter was analyzed in Casuarinaceae. We observed that CgAUX1 was expressed in plant cells infected by Frankia throughout the course of actinorhizal nodule formation. Our data suggest that auxin plays an important role during plant cell infection in actinorhizal symbioses.
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Auxin Influx Activity Is Associated with Frankia Infection during Actinorhizal Nodule Formation in
2007Co-Authors: Ranjan Swarup, Claudine Franche, Carole Santi, Didier Bogusz, Florence Auguy, L Jansen, Myriam Collin, Malcolm J. Bennett, Laurent LaplazeAbstract:Plants from the Casuarinaceae family enter symbiosis with the actinomycete Frankia leading to the formation of nitrogen-fixing root nodules. We observed that application of the auxin influx inhibitor 1-naphtoxyacetic acid perturbs actinorhizal nodule formation. This suggests a potential role for auxin influx carriers in the infection process. We therefore isolated and characterized homologs of the auxin influx carrier (AUX1-LAX) genes in Casuarina glauca. Two members of this family were found to share high levels of deduced protein sequence identity with Arabidopsis (Arabidopsis thaliana) AUX-LAX proteins. Complementation of the Arabidopsis aux1 mutant revealed that one of them is functionally equivalent to AUX1 and was named CgAUX1. The spatial and temporal expression pattern of CgAUX1 promoter:b-glucuronidase reporter was analyzed in Casuarinaceae. We observed that CgAUX1 was expressed in plant cells infected by Frankia throughout the course of actinorhizal nodule formation. Our data suggest that auxin plays an important role during plant cell infection in actinorhizal symbioses. Actinorhizal plants, which belong to eight families of angiosperms, can form nitrogen-fixing nodules in symbiosis with the soil actinomycete Frankia (Benson and Silvester, 1993). The symbiotic interaction starts, under conditions of nitrogen deprivation, by an exchange of signals between the plant roots and bacteria. The chemical nature of Frankia nodulation factors is unknown, but data suggest that it has different bio
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cg12 expression is specifically linked to infection of root hairs and cortical cells during casuarina glauca and allocasuarina verticillata actinorhizal nodule development
Molecular Plant-microbe Interactions, 2003Co-Authors: Sergio Svistoonoff, Claudine Franche, Florence Auguy, Laurent Laplaze, John Runions, Robin Duponnois, Jim Haseloff, Didier BoguszAbstract:cg12 is an early actinorhizal nodulin gene from Casuarina glauca encoding a subtilisin-like serine protease. Using transgenic Casuarinaceae plants carrying cg12-gus and cg12-gfp fusions, we have studied the expression pattern conferred by the cg12 promoter region after inoculation with Frankia. cg12 was found to be expressed in root hairs and in root and nodule cortical cells containing Frankia infection threads. cg12 expression was also monitored after inoculation with ineffective Frankia strains, during my-corrhizae formation, and after diverse hormonal treatments. None of these treatments was able to induce its expression, therefore suggesting that cg12 expression is linked to plant cell infection by Frankia strains. Possible roles of cg12 in actinorhizal symbiosis are discussed.
Florence Auguy - One of the best experts on this subject based on the ideXlab platform.
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Plant and Soil 254: 229–237, 2003. © 2003 Kluwer Academic Publishers. Printed in the Netherlands.
2013Co-Authors: Carole Santi, Didier Bogusz, Florence Auguy, Emile Duhoux, Sergio Svistoonoff, Laure Constans, Claudine FrancheAbstract:Choosing a reporter for gene expression studies in transgenic actinorhizal plants of the Casuarinaceae famil
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Contribution of transgenic Casuarinaceae to our knowledge of the actinorhizal symbioses
Symbiosis, 2010Co-Authors: Sergio Svistoonoff, Valérie Hocher, Florence Auguy, Laurent Laplaze, Hassen Gherbi, Mathish Nambiar-veetil, Chonglu Zhong, Zuzanna Michalak, Virginie Vaissayre, Patrick DoumasAbstract:The Casuarinaceae family is a group of 96 species of trees and shrubs that are tolerant to adverse soil and climatic conditions. In the field, Casuarinaceae bears nitrogen-fixing root nodules (so called actinorhizal nodules) resulting from infection by the soil actinomycete Frankia. The association between Casuarina and Frankia is of tremendous ecological importance in tropical and subtropical areas where these trees contribute to land stabilization and soil reclamation. During differentiation of the actinorhizal nodule, a set of genes called actinorhizal nodulins is activated in the developing nodule. Understanding the molecular basis of actinorhizal nodule ontogenesis requires molecular tools such as genomics together with gene transfer technologies for functional analysis of symbiotic genes. Using the biological vectors Agrobacterium rhizogenes and A. tumefaciens, gene transfer into the two species Allocasuarina verticillata and Casuarina glauca has been successful. Transgenic Casuarinaceae plants proved to be valuable tools for exploring the molecular mechanisms resulting from the infection process of actinorhizal plants by Frankia.
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Auxin Influx Activity Is Associated with Frankia Infection during Actinorhizal Nodule Formation in Casuarina glauca
PLANT PHYSIOLOGY, 2007Co-Authors: Benjamin Peret, Gaetan Devos, Claudine Franche, Carole Santi, Valérie Hocher, Florence Auguy, Ranjan Swarup, L Jansen, Matthew Collin, Didier BoguszAbstract:Plants from the Casuarinaceae family enter symbiosis with the actinomycete Frankia leading to the formation of nitrogen-fixing root nodules. We observed that application of the auxin influx inhibitor 1-naphtoxyacetic acid perturbs actinorhizal nodule formation. This suggests a potential role for auxin influx carriers in the infection process. We therefore isolated and characterized homologs of the auxin influx carrier (AUX1-LAX) genes in Casuarina glauca. Two members of this family were found to share high levels of deduced protein sequence identity with Arabidopsis (Arabidopsis thaliana) AUX-LAX proteins. Complementation of the Arabidopsis aux1 mutant revealed that one of them is functionally equivalent to AUX1 and was named CgAUX1. The spatial and temporal expression pattern of CgAUX1 promoter:beta-glucuronidase reporter was analyzed in Casuarinaceae. We observed that CgAUX1 was expressed in plant cells infected by Frankia throughout the course of actinorhizal nodule formation. Our data suggest that auxin plays an important role during plant cell infection in actinorhizal symbioses.
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Auxin Influx Activity Is Associated with Frankia Infection during Actinorhizal Nodule Formation in
2007Co-Authors: Ranjan Swarup, Claudine Franche, Carole Santi, Didier Bogusz, Florence Auguy, L Jansen, Myriam Collin, Malcolm J. Bennett, Laurent LaplazeAbstract:Plants from the Casuarinaceae family enter symbiosis with the actinomycete Frankia leading to the formation of nitrogen-fixing root nodules. We observed that application of the auxin influx inhibitor 1-naphtoxyacetic acid perturbs actinorhizal nodule formation. This suggests a potential role for auxin influx carriers in the infection process. We therefore isolated and characterized homologs of the auxin influx carrier (AUX1-LAX) genes in Casuarina glauca. Two members of this family were found to share high levels of deduced protein sequence identity with Arabidopsis (Arabidopsis thaliana) AUX-LAX proteins. Complementation of the Arabidopsis aux1 mutant revealed that one of them is functionally equivalent to AUX1 and was named CgAUX1. The spatial and temporal expression pattern of CgAUX1 promoter:b-glucuronidase reporter was analyzed in Casuarinaceae. We observed that CgAUX1 was expressed in plant cells infected by Frankia throughout the course of actinorhizal nodule formation. Our data suggest that auxin plays an important role during plant cell infection in actinorhizal symbioses. Actinorhizal plants, which belong to eight families of angiosperms, can form nitrogen-fixing nodules in symbiosis with the soil actinomycete Frankia (Benson and Silvester, 1993). The symbiotic interaction starts, under conditions of nitrogen deprivation, by an exchange of signals between the plant roots and bacteria. The chemical nature of Frankia nodulation factors is unknown, but data suggest that it has different bio
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cg12 expression is specifically linked to infection of root hairs and cortical cells during casuarina glauca and allocasuarina verticillata actinorhizal nodule development
Molecular Plant-microbe Interactions, 2003Co-Authors: Sergio Svistoonoff, Claudine Franche, Florence Auguy, Laurent Laplaze, John Runions, Robin Duponnois, Jim Haseloff, Didier BoguszAbstract:cg12 is an early actinorhizal nodulin gene from Casuarina glauca encoding a subtilisin-like serine protease. Using transgenic Casuarinaceae plants carrying cg12-gus and cg12-gfp fusions, we have studied the expression pattern conferred by the cg12 promoter region after inoculation with Frankia. cg12 was found to be expressed in root hairs and in root and nodule cortical cells containing Frankia infection threads. cg12 expression was also monitored after inoculation with ineffective Frankia strains, during my-corrhizae formation, and after diverse hormonal treatments. None of these treatments was able to induce its expression, therefore suggesting that cg12 expression is linked to plant cell infection by Frankia strains. Possible roles of cg12 in actinorhizal symbiosis are discussed.
Emile Duhoux - One of the best experts on this subject based on the ideXlab platform.
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Plant and Soil 254: 229–237, 2003. © 2003 Kluwer Academic Publishers. Printed in the Netherlands.
2013Co-Authors: Carole Santi, Didier Bogusz, Florence Auguy, Emile Duhoux, Sergio Svistoonoff, Laure Constans, Claudine FrancheAbstract:Choosing a reporter for gene expression studies in transgenic actinorhizal plants of the Casuarinaceae famil
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Choosing a reporter for gene expression studies in transgenic actinorhizal plants of the Casuarinaceae family
Frankia Symbiosis, 2003Co-Authors: Carole Santi, Didier Bogusz, Florence Auguy, Emile Duhoux, Sergio Svistoonoff, Laure Constans, Claudine FrancheAbstract:Transgenic Casuarinaceae and reporter genes provide valuable tools to study gene expression in transgenic actinorhizal nodules. In this paper, we discuss the use of s-glucuronidase for the histochemical localization and quantification of gene expression in transgenic plants of Allocasuarina verticillata and Casuarina glauca nodulated by the actinomycete Frankia. We also report on the genetic transformation of A. verticillata by the Agrobacterium tumefaciens strain C58C1(pGV2260) containing the 35S-mgfp5-ER construct encoding a modified green fluorescent protein of Aequorea victoria in a binary vector. The evolution of the GFP fluorescence was monitored through all stages of the regeneration process. The data indicate that GFP is not toxic in Casuarinaceae and that this reporter gene can be used for visual screening of transformed calli and transgenic plants. The fluorescence pattern of gfp provides a new tool for monitoring in vivo transgenc expression in actinorhizal plants.
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symbiotic and non symbiotic expression of cgmt1 a metallothionein like gene from the actinorhizal tree casuarina glauca
Plant Molecular Biology, 2002Co-Authors: Laurent Laplaze, Claudine Franche, Florence Auguy, Katharina Pawlowski, Emile Duhoux, H. Gherbi, Fathia Guermache, Didier BoguszAbstract:A clone for a type 1 metallothionein (cgMT1) was isolated from a Casuarina glauca nodule cDNA library. The corresponding gene belongs to a small family and is highly expressed in roots and nitrogen-fixing nodules, whereas low expression was observed in aerial parts of the plant. The promoter region of cgMT1 was isolated and fused to the β-glucuronidase (gus) gene. Transgenic Casuarinaceae plants showed that the cgMT1 promoter was most active in roots and in the oldest region of the shoot. In situ hybridization indicated that in nodules cgMT1 transcript is present in mature Frankia-infected cells and in the pericycle. Possible roles for cgMT1 in symbiotic and non-symbiotic tissues are discussed.
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Characterization of a Casuarina glauca Nodule-Specific Subtilisin-like Protease Gene, a Homolog of Alnus glutinosa ag12
Molecular plant-microbe interactions : MPMI, 2000Co-Authors: Laurent Laplaze, Claudine Franche, Didier Bogusz, Florence Auguy, Ana Ribeiro, Emile Duhoux, Katharina PawlowskiAbstract:In search of plant genes expressed during early interactions between Casuarina glauca and Frankia, we have isolated and characterized a C. glauca gene that has strong homology to subtilisin-like protease gene families of several plants including the actinorhizal nodulin gene ag12 of another actinorhizal plant, Alnus glutinosa. Based on the expression pattern of cg12 in the course of nodule development, it represents an early actinorhizal nodulin gene. Our results suggest that subtilisin-like proteases may be a common element in the process of infection of plant cells by Frankia in both Betulaceae (Alnus glutinosa) and Casuarinaceae (Casuarina glauca) symbioses.
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Molecular Biology of Tropical Nitrogen-Fixing Trees in the Casuarinaceae Family
Molecular Biology of Woody Plants, 2000Co-Authors: Laurent Laplaze, Florence Auguy, Marie-claude Bon, Aziz Smouni, Christelle Allonneau, Thierry Frutz, Maryannick Rio, Fatiha Guermache, Emile DuhouxAbstract:The Casuarinaceae family includes about eighty species of shrubs and trees belonging to four genera, namely: Allocasuarina, Casuarina, Ceuthostoma, and Gymnostoma. Casuarinaceae are primarily native to the Southern Hemisphere, mostly Australia and Indo-Pacific areas, from Malaysia to Polynesia. However, the range of distribution of some genera such as Casuarina has been extended considerably through artificial dissemination. All members of the family are characterized by highly reduced leaves and photosynthetic deciduous branchlets (Midgley et al., 1983; National Research Council, 1984).