The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform
Robert Verpoorte - One of the best experts on this subject based on the ideXlab platform.
-
Cell and tissue cultures of Catharanthus roseus: A literature survey
Plant Cell Tissue and Organ Culture, 1995Co-Authors: Paulo R. H. Moreno, Robert Heijden, Robert VerpoorteAbstract:The literature concerning the regulation and the biosynthesis of secondary metabolites in cell and tissue cultures of Catharanthus roseus is reviewed. The aim of this review is to summarise the progress achieved since the previous review of this subject from 1988 to December 1993. Several factors influencing the production of indole alkaloids are discussed. Special attention is given to large-scale cultivation methods. Some economic considerations on the production of ajmalicine are also discussed.
-
Ajmalicine metabolism in Catharanthus roseus cell cultures
Phytochemistry, 1994Co-Authors: Rosana I. Dos Santos, Jan Schripsema, Robert VerpoorteAbstract:Abstract Ajmalicine degradation was investigated in a low alkaloid-accumulating Catharanthus roseus cell culture, and compared with the chemical degradation which occurred in medium without cells. Degradation was found to be faster in the presence of cells. By adding [Me- 2 H 3 ] ajmalicine to the cell culture the production and the degradation rates were estimated. Both were relatively high at the beginning of the growth phase while the degradation rate was also high in the stationary phase.
-
Purification of the cytochrome P-450 enzyme geraniol 10-hydroxylase from cell cultures of Catharanthus roseus
Journal of Chromatography A, 1993Co-Authors: Annemarie H. Meijer, Anthony De Waal, Robert VerpoorteAbstract:Abstract The cytochrome P-450 enzyme geraniol 10-hydroxylase (G10H) was purified from a suspension culture of Catharanthus roseus, grown on an alkaloid prod
Hiroki Hamada - One of the best experts on this subject based on the ideXlab platform.
-
Biotransformation of geraniol, nerol and (+)- and (−)-carvone by suspension cultured cells of Catharanthus roseus
Phytochemistry, 1997Co-Authors: Hiroki Hamada, Toshifumi Hirata, Yoshihiro Fuchikami, Howard J. Williams, Hideaki Yasumune, Isabel Sattler, A. Ian ScottAbstract:Abstract Suspension cultured cells of Catharanthus roseus hydroxylate the allylic positions of geraniol, nerol and (+)- and (−)-carvone and reduce double bonds and ketone groups. After incubation for 7 days, the main products of (−)- and (+)-carvone were 5β-hydroxyneodihydrocarveol and 5α-hydroxycarvone, respectively.
-
Hydroxylation of piperitone by cell suspension cultures of Catharanthus roseus
Phytochemistry, 1994Co-Authors: Hiroki Hamada, Toshifumi Hirata, Yoshihiro Fuchikami, Yuka Ikematsu, Howard J. Williams, A. Ian ScottAbstract:Abstract Cell suspension cultures of Catharanthus roseus regioselectively hydroxylate the 4- and 6-positions of (−)-piperitone.
-
Regioselective hydroxylation of warfarin by cell suspension cultures of Catharanthus roseus
Phytochemistry, 1993Co-Authors: Hiroki Hamada, Yoshihiro Fuchikami, Robert L. Jansing, Laurence S. KaminskyAbstract:Abstract Cell suspension cultures of Catharanthus roseus reduce the carbonyl group of warfarin to the corresponding alcohol and regioselectively hydroxylate warfarin.
A. Ian Scott - One of the best experts on this subject based on the ideXlab platform.
-
Biotransformation of geraniol, nerol and (+)- and (−)-carvone by suspension cultured cells of Catharanthus roseus
Phytochemistry, 1997Co-Authors: Hiroki Hamada, Toshifumi Hirata, Yoshihiro Fuchikami, Howard J. Williams, Hideaki Yasumune, Isabel Sattler, A. Ian ScottAbstract:Abstract Suspension cultured cells of Catharanthus roseus hydroxylate the allylic positions of geraniol, nerol and (+)- and (−)-carvone and reduce double bonds and ketone groups. After incubation for 7 days, the main products of (−)- and (+)-carvone were 5β-hydroxyneodihydrocarveol and 5α-hydroxycarvone, respectively.
-
Hydroxylation of piperitone by cell suspension cultures of Catharanthus roseus
Phytochemistry, 1994Co-Authors: Hiroki Hamada, Toshifumi Hirata, Yoshihiro Fuchikami, Yuka Ikematsu, Howard J. Williams, A. Ian ScottAbstract:Abstract Cell suspension cultures of Catharanthus roseus regioselectively hydroxylate the 4- and 6-positions of (−)-piperitone.
Yoshihiro Fuchikami - One of the best experts on this subject based on the ideXlab platform.
-
Biotransformation of geraniol, nerol and (+)- and (−)-carvone by suspension cultured cells of Catharanthus roseus
Phytochemistry, 1997Co-Authors: Hiroki Hamada, Toshifumi Hirata, Yoshihiro Fuchikami, Howard J. Williams, Hideaki Yasumune, Isabel Sattler, A. Ian ScottAbstract:Abstract Suspension cultured cells of Catharanthus roseus hydroxylate the allylic positions of geraniol, nerol and (+)- and (−)-carvone and reduce double bonds and ketone groups. After incubation for 7 days, the main products of (−)- and (+)-carvone were 5β-hydroxyneodihydrocarveol and 5α-hydroxycarvone, respectively.
-
Hydroxylation of piperitone by cell suspension cultures of Catharanthus roseus
Phytochemistry, 1994Co-Authors: Hiroki Hamada, Toshifumi Hirata, Yoshihiro Fuchikami, Yuka Ikematsu, Howard J. Williams, A. Ian ScottAbstract:Abstract Cell suspension cultures of Catharanthus roseus regioselectively hydroxylate the 4- and 6-positions of (−)-piperitone.
-
Regioselective hydroxylation of warfarin by cell suspension cultures of Catharanthus roseus
Phytochemistry, 1993Co-Authors: Hiroki Hamada, Yoshihiro Fuchikami, Robert L. Jansing, Laurence S. KaminskyAbstract:Abstract Cell suspension cultures of Catharanthus roseus reduce the carbonyl group of warfarin to the corresponding alcohol and regioselectively hydroxylate warfarin.
Iyarajan - One of the best experts on this subject based on the ideXlab platform.
-
Influence of Brassinosteroids (BRs) on the vincristine content of Catharanthus roseus (L). G. Don
European Journal of Experimental Biology, 2015Co-Authors: S. Muthulakshmi, IyarajanAbstract:Catharanthus roseus (L). G. Don is an important anticancerous plant belonging to the family Apocynaceae. It contains so many alkaloids like Vinblastine and vincristine are mainly present in aerial parts of C. roseus, which are used in treatment of various human cancers, so it is considered as mile stone in cancer chemotherapy. Vincristine is also used to cure cancer including acute leukemia, Hodgkin's and non- Hodgkin's lymphoma, neuroblastoma, rhabdomyosarcoma, Ewing's sarcoma, Wilms' tumor, multiple myeloma, chronic leukemias, thyroid cancer, brain tumors and blood disorders. In our study the effect of BRs on vincristine content of Catharanthus roseus was quantified. BRswas applied as foliar spray at different concentrations viz 5, 10, 15, and 20 ppm for 30days regularly. The vincristine content was quantified by HPLC method. The vincristine content was found to increase at all the concentration when compared to the control. The maximum concentration of vincristine was found to be around 15 and 20 ppm of BRs. Hence it is concluded that regular application of BRs to Catharanthus roseus can be recommended for attaining better biomass production and vincristine content.
-
Influence of IAA on the vincristine content of Catharanthus roseus (L). G. Don.
Asian Journal of Plant Science & Research, 2013Co-Authors: S. Muthulakshmi, IyarajanAbstract:Catharanthus roseus (L). G. Don is an important anticancerous plant belonging to the family Apocyanaceae. It contains so many alkaloids like vincristine, vinblastine and ajmalcine which are pharmacologically important. This plant may be considered as a famous chemical factory for biosynthesis of a huge array of alkaloids and many of these chemicals are utilized as medicines. The effect of IAA on vincristine content of Catharanthus roseus was studied. IAA was applied as foliar spray at different concentrations viz 50, 100, 150, and 200ppm for 30days. Vincristine was quantified by HPLC method. Vincristine was significantly maximum at 150 and 200ppm concentration of IAA when compared to other concentrations and control. Hence it is concluded that practice of application of IAA to Catharanthus roseus can be recommended for attaining better biomass production and vincristine content.