The Experts below are selected from a list of 2205 Experts worldwide ranked by ideXlab platform
Taymour Mostafa - One of the best experts on this subject based on the ideXlab platform.
-
Cavernosal hydrogen sulfide levels are associated with nitric oxide and hemeoxygenase levels in diabetic rats
International Journal of Impotence Research, 2019Co-Authors: Taymour Mostafa, Laila Rashed, Nashaat Nabil, Ahmed F. Abo-sief, Mai M. Mohamed, Maroa S. OmarAbstract:Penile erection is a neuromuscular event modulated by psyche, hormones as well as neurotransmitters. This pre-clinical study aimed to assess hydrogen sulfide (H_2S) relationship with nitric oxide (NO) and hemeoxygenase (HO) in the Cavernous Tissues of diabetic rats. Overall, 90 adult male rats were investigated (6 groups, n = 15 each). They were subdivided into the following groups; untreated rats, rats treated with H_2S donor/inhibitor, induced diabetic rats, diabetic rats treated with H_2S donor/inhibitor. At the 6th week, the rats were killed to assess Cavernous Tissue cGMP, NO, H_2S, HO enzyme activity levels. The rats treated with H_2S donor showed increased mean Cavernous Tissue cGMP, NO, H_2S, and HO enzyme activity levels whereas induced diabetic rats and rats treated with H_2S inhibitor showed significant decreases in these parameters compared with the untreated rats. On the other hands, diabetic rats treated with H_2S donor showed elevated mean Cavernous Tissue cGMP, NO, H_2S, and HO enzyme activity levels whereas diabetic rats treated with H_2S inhibitor showed significant decreases in these parameters compared with diabetic rats. Cavernous Tissue H_2S levels exhibited significant positive correlations with the Cavernous Tissue levels cGMP, NO, and HO enzyme activity levels. From these results, it could be concluded that Cavernous Tissues H_2S plays a role of male sexual health by affecting Cavernous Tissues NO and HO enzyme activity in general and in diabetics in particular.
-
spontaneous penile tumescence by sparing Cavernous Tissue in the course of malleable penile prosthesis implantation
The Journal of Sexual Medicine, 2019Co-Authors: Adham Zaazaa, Taymour MostafaAbstract:Abstract Introduction Spontaneous penile tumescence after penile prosthesis implantation has been sporadically reported in the literature. Aim To preserve residual erectile function of patients’ spontaneous penile tumescence by sparing Cavernous Tissue in the course of malleable penile prosthesis implantation. Methods Overall, 92 patients were randomized into 2 equal arms; patients undergoing conventional malleable penile prosthesis implantation, and patients undergoing the Cavernous Tissue–sparing technique. 1 month after surgery, these patients underwent penile duplex examination to assess the maximal Cavernous Tissue thickness around the implant cylinders. Additionally, they were asked about the occurrence of any spontaneous or arousal-induced penile tumescence. Main Outcome Measures Postoperative changes were compared with the preoperative ones. Results The mean maximal Cavernous Tissue thickness was shown to be significantly higher in the Cavernous Tissue–sparing group compared with the conventional surgery group (5.2 ± 0.8 mm vs 2.2 ± 1.04 mm, P Clinical Implications This study provides a step-by-step approach to maintaining post-implantation penile tumescence and preserving penile girth in a reproducible manner. Strengths & Limitations This is the first study to demonstrate the benefits of implanting a penile prosthesis while the penis is in a pharmacologically induced tumescent state. It is also the first to make use of ultrasound imaging in assessing postoperative corporal Tissue. The main limitations are the short postoperative follow-up period and the non-blinding of measurements. Conclusion It could be concluded that the Cavernous Tissue–sparing technique is a reproducible technique that has the added value of preserving residual erectile function in the form of retained postoperative penile tumescence and preserved penile girth. Zaazaa A, Mostafa T. Spontaneous Penile Tumescence by Sparing Cavernous Tissue in the Course of Malleable Penile Prosthesis Implantation. J Sex Med 2019;16:474–478.
-
spontaneous penile tumescence by sparing Cavernous Tissue in the course of malleable penile prosthesis implantation
The Journal of Sexual Medicine, 2019Co-Authors: Adham Zaazaa, Taymour MostafaAbstract:INTRODUCTION: Spontaneous penile tumescence after penile prosthesis implantation has been sporadically reported in the literature. AIM: To preserve residual erectile function of patients' spontaneous penile tumescence by sparing Cavernous Tissue in the course of malleable penile prosthesis implantation. METHODS: Overall, 92 patients were randomized into 2 equal arms; patients undergoing conventional malleable penile prosthesis implantation, and patients undergoing the Cavernous Tissue-sparing technique. 1 month after surgery, these patients underwent penile duplex examination to assess the maximal Cavernous Tissue thickness around the implant cylinders. Additionally, they were asked about the occurrence of any spontaneous or arousal-induced penile tumescence. MAIN OUTCOME MEASURES: Postoperative changes were compared with the preoperative ones. RESULTS: The mean maximal Cavernous Tissue thickness was shown to be significantly higher in the Cavernous Tissue-sparing group compared with the conventional surgery group (5.2 ± 0.8 mm vs 2.2 ± 1.04 mm, P < .01). In the Cavernous Tissue-sparing group, 41 of 46 patients (89.13%) reported having a significantly higher incidence of residual penile tumescence vs 7 of 46 patients (15.2%) in the conventional surgery group (P < .001). The postoperative penile girth was significantly higher in the Cavernous Tissue-sparing group than in the conventional surgery group (11.16 ± 1.1 cm vs 10.11 ± 1.15 cm, P < .001). CLINICAL IMPLICATIONS: This study provides a step-by-step approach to maintaining post-implantation penile tumescence and preserving penile girth in a reproducible manner. STRENGTHS & LIMITATIONS: This is the first study to demonstrate the benefits of implanting a penile prosthesis while the penis is in a pharmacologically induced tumescent state. It is also the first to make use of ultrasound imaging in assessing postoperative corporal Tissue. The main limitations are the short postoperative follow-up period and the non-blinding of measurements. CONCLUSION: It could be concluded that the Cavernous Tissue-sparing technique is a reproducible technique that has the added value of preserving residual erectile function in the form of retained postoperative penile tumescence and preserved penile girth. Zaazaa A, Mostafa T. Spontaneous Penile Tumescence by Sparing Cavernous Tissue in the Course of Malleable Penile Prosthesis Implantation. J Sex Med 2019;16:474-478.
-
Spontaneous Penile Tumescence by Sparing Cavernous Tissue in the Course of Malleable Penile Prosthesis Implantation.
The Journal of Sexual Medicine, 2019Co-Authors: Adham Zaazaa, Taymour MostafaAbstract:INTRODUCTION: Spontaneous penile tumescence after penile prosthesis implantation has been sporadically reported in the literature. AIM: To preserve residual erectile function of patients' spontaneous penile tumescence by sparing Cavernous Tissue in the course of malleable penile prosthesis implantation. METHODS: Overall, 92 patients were randomized into 2 equal arms; patients undergoing conventional malleable penile prosthesis implantation, and patients undergoing the Cavernous Tissue-sparing technique. 1 month after surgery, these patients underwent penile duplex examination to assess the maximal Cavernous Tissue thickness around the implant cylinders. Additionally, they were asked about the occurrence of any spontaneous or arousal-induced penile tumescence. MAIN OUTCOME MEASURES: Postoperative changes were compared with the preoperative ones. RESULTS: The mean maximal Cavernous Tissue thickness was shown to be significantly higher in the Cavernous Tissue-sparing group compared with the conventional surgery group (5.2 ± 0.8 mm vs 2.2 ± 1.04 mm, P
-
effect of testosterone and frequent low dose sildenafil tadalafil on Cavernous Tissue oxidative stress of aged diabetic rats
Andrologia, 2012Co-Authors: Taymour Mostafa, L. A. Rashed, K. Kotb, Mai TaymourAbstract:Summary This study aimed to assess the effect of testosterone (T) administration and chronic low-dose sildenafil/tadalafil on Cavernous Tissue oxidative stress (OS) of aged diabetic rats. In all, 140 Sprague-Dawley aged rats were subdivided into the following: controls; streptozotocin (STZ)-induced diabetic rats; diabetic rats injected with T every 4 weeks; diabetic rats on sildenafil orally daily; diabetic rats on T and daily sildenafil; diabetic rats on tadalafil orally every other day; diabetic rats on T and tadalafil; diabetic rats on alternate sildenafil/tadalafil; and diabetic rats on alternate sildenafil/tadalafil with T. After 12 weeks, the rats were euthanised where in dissected Cavernous Tissues malondialdehyde (MAD), glutathione peroxidase (GPx) and cGMP (cyclic guanosine monophosphate) were estimated. Compared with controls, aged diabetic rats demonstrated significant increase in Cavernous Tissue MDA and significant decrease in GPx and cGMP where diabetic rats injected with T had marked improvement of these parameters. Diabetic rats on sildenafil, tadalafil or alternate sildenafil/tadalafil demonstrated significant increased Cavernous Tissue GPx, cGMP and decreased Cavernous MDA that was further improved when supplemented with T. It is concluded that frequent low-dose use of sildenafil and/or tadalafil supplemented with T has a marked impact on ameliorating Cavernous OS in aged diabetic rats.
Mohammed S Elsheemy - One of the best experts on this subject based on the ideXlab platform.
-
are the Cavernous Tissue and serum levels of micro rnas 200a and 206 elevated in patients with refractory veno occlusive erectile dysfunction a comparative study
Urology, 2017Co-Authors: Sameh Fayek Gamalel Din, Laila A Rashed, Hany Ahmed Fouad Alghobary, Lamiaa Tarek Tawfik, Mohammed S ElsheemyAbstract:OBJECTIVE To evaluate the association between miRNAs and veno-occlusive erectile dysfunction. Recently, this association between miRNAs and erectile dysfunction was extensively studied using animal models. Our aim was to explore the miRNAs expressions and functions in the development of erectile dysfunction, especially veno-occlusive dysfunction, using a human Tissue. PATIENTS AND METHODS We prospectively recruited 60 patients with erectile dysfunction and controls between July 2015 and July 2016. The 30 patients had refractory veno-occlusive erectile dysfunction that was proven by investigations. They were scheduled for penile implant. The 30 controls were scheduled for repair of their fracture. We measured miRNAs (200a and 206) and nitric oxide in Cavernous Tissue and serum of both patients with erectile dysfunction and controls. RESULTS A significant association was found between the 2 mentioned miRNAs and erectile dysfunction (P <.001). Mean level of nitric oxide in Cavernous Tissue of the controls was significantly higher than that in the patients (P <.001). miRNA 200a showed a cutoff value of 1.135 with 95% sensitivity and 100% specificity, whereas miRNA 206 showed a cutoff value of 1.125 with 100% sensitivity and 100% specificity. CONCLUSION To the best of our knowledge, our study is the first report to measure the level of miRNAs in the Cavernous Tissue, using a human Tissue. Furthermore, this study can be considered a good step of deploying miRNAs through a blood test to detect early negative changes that lead to erectile dysfunction. Finally, we recommend more studies to be conducted to better understand if these miRNAs are involved in the pathophysiology of veno-occlusive erectile dysfunction.
-
Are the Cavernous Tissue and Serum Levels of Micro RNAs 200a and 206 Elevated in Patients With Refractory Veno-occlusive Erectile Dysfunction? A Comparative Study.
Urology, 2017Co-Authors: Sameh Fayek Gamalel Din, Laila A Rashed, Hany Ahmed Fouad Alghobary, Lamiaa Tarek Tawfik, Mohammed S ElsheemyAbstract:OBJECTIVE To evaluate the association between miRNAs and veno-occlusive erectile dysfunction. Recently, this association between miRNAs and erectile dysfunction was extensively studied using animal models. Our aim was to explore the miRNAs expressions and functions in the development of erectile dysfunction, especially veno-occlusive dysfunction, using a human Tissue. PATIENTS AND METHODS We prospectively recruited 60 patients with erectile dysfunction and controls between July 2015 and July 2016. The 30 patients had refractory veno-occlusive erectile dysfunction that was proven by investigations. They were scheduled for penile implant. The 30 controls were scheduled for repair of their fracture. We measured miRNAs (200a and 206) and nitric oxide in Cavernous Tissue and serum of both patients with erectile dysfunction and controls. RESULTS A significant association was found between the 2 mentioned miRNAs and erectile dysfunction (P
Adham Zaazaa - One of the best experts on this subject based on the ideXlab platform.
-
spontaneous penile tumescence by sparing Cavernous Tissue in the course of malleable penile prosthesis implantation
The Journal of Sexual Medicine, 2019Co-Authors: Adham Zaazaa, Taymour MostafaAbstract:Abstract Introduction Spontaneous penile tumescence after penile prosthesis implantation has been sporadically reported in the literature. Aim To preserve residual erectile function of patients’ spontaneous penile tumescence by sparing Cavernous Tissue in the course of malleable penile prosthesis implantation. Methods Overall, 92 patients were randomized into 2 equal arms; patients undergoing conventional malleable penile prosthesis implantation, and patients undergoing the Cavernous Tissue–sparing technique. 1 month after surgery, these patients underwent penile duplex examination to assess the maximal Cavernous Tissue thickness around the implant cylinders. Additionally, they were asked about the occurrence of any spontaneous or arousal-induced penile tumescence. Main Outcome Measures Postoperative changes were compared with the preoperative ones. Results The mean maximal Cavernous Tissue thickness was shown to be significantly higher in the Cavernous Tissue–sparing group compared with the conventional surgery group (5.2 ± 0.8 mm vs 2.2 ± 1.04 mm, P Clinical Implications This study provides a step-by-step approach to maintaining post-implantation penile tumescence and preserving penile girth in a reproducible manner. Strengths & Limitations This is the first study to demonstrate the benefits of implanting a penile prosthesis while the penis is in a pharmacologically induced tumescent state. It is also the first to make use of ultrasound imaging in assessing postoperative corporal Tissue. The main limitations are the short postoperative follow-up period and the non-blinding of measurements. Conclusion It could be concluded that the Cavernous Tissue–sparing technique is a reproducible technique that has the added value of preserving residual erectile function in the form of retained postoperative penile tumescence and preserved penile girth. Zaazaa A, Mostafa T. Spontaneous Penile Tumescence by Sparing Cavernous Tissue in the Course of Malleable Penile Prosthesis Implantation. J Sex Med 2019;16:474–478.
-
spontaneous penile tumescence by sparing Cavernous Tissue in the course of malleable penile prosthesis implantation
The Journal of Sexual Medicine, 2019Co-Authors: Adham Zaazaa, Taymour MostafaAbstract:INTRODUCTION: Spontaneous penile tumescence after penile prosthesis implantation has been sporadically reported in the literature. AIM: To preserve residual erectile function of patients' spontaneous penile tumescence by sparing Cavernous Tissue in the course of malleable penile prosthesis implantation. METHODS: Overall, 92 patients were randomized into 2 equal arms; patients undergoing conventional malleable penile prosthesis implantation, and patients undergoing the Cavernous Tissue-sparing technique. 1 month after surgery, these patients underwent penile duplex examination to assess the maximal Cavernous Tissue thickness around the implant cylinders. Additionally, they were asked about the occurrence of any spontaneous or arousal-induced penile tumescence. MAIN OUTCOME MEASURES: Postoperative changes were compared with the preoperative ones. RESULTS: The mean maximal Cavernous Tissue thickness was shown to be significantly higher in the Cavernous Tissue-sparing group compared with the conventional surgery group (5.2 ± 0.8 mm vs 2.2 ± 1.04 mm, P < .01). In the Cavernous Tissue-sparing group, 41 of 46 patients (89.13%) reported having a significantly higher incidence of residual penile tumescence vs 7 of 46 patients (15.2%) in the conventional surgery group (P < .001). The postoperative penile girth was significantly higher in the Cavernous Tissue-sparing group than in the conventional surgery group (11.16 ± 1.1 cm vs 10.11 ± 1.15 cm, P < .001). CLINICAL IMPLICATIONS: This study provides a step-by-step approach to maintaining post-implantation penile tumescence and preserving penile girth in a reproducible manner. STRENGTHS & LIMITATIONS: This is the first study to demonstrate the benefits of implanting a penile prosthesis while the penis is in a pharmacologically induced tumescent state. It is also the first to make use of ultrasound imaging in assessing postoperative corporal Tissue. The main limitations are the short postoperative follow-up period and the non-blinding of measurements. CONCLUSION: It could be concluded that the Cavernous Tissue-sparing technique is a reproducible technique that has the added value of preserving residual erectile function in the form of retained postoperative penile tumescence and preserved penile girth. Zaazaa A, Mostafa T. Spontaneous Penile Tumescence by Sparing Cavernous Tissue in the Course of Malleable Penile Prosthesis Implantation. J Sex Med 2019;16:474-478.
-
Spontaneous Penile Tumescence by Sparing Cavernous Tissue in the Course of Malleable Penile Prosthesis Implantation.
The Journal of Sexual Medicine, 2019Co-Authors: Adham Zaazaa, Taymour MostafaAbstract:INTRODUCTION: Spontaneous penile tumescence after penile prosthesis implantation has been sporadically reported in the literature. AIM: To preserve residual erectile function of patients' spontaneous penile tumescence by sparing Cavernous Tissue in the course of malleable penile prosthesis implantation. METHODS: Overall, 92 patients were randomized into 2 equal arms; patients undergoing conventional malleable penile prosthesis implantation, and patients undergoing the Cavernous Tissue-sparing technique. 1 month after surgery, these patients underwent penile duplex examination to assess the maximal Cavernous Tissue thickness around the implant cylinders. Additionally, they were asked about the occurrence of any spontaneous or arousal-induced penile tumescence. MAIN OUTCOME MEASURES: Postoperative changes were compared with the preoperative ones. RESULTS: The mean maximal Cavernous Tissue thickness was shown to be significantly higher in the Cavernous Tissue-sparing group compared with the conventional surgery group (5.2 ± 0.8 mm vs 2.2 ± 1.04 mm, P
Laila A Rashed - One of the best experts on this subject based on the ideXlab platform.
-
are the Cavernous Tissue and serum levels of micro rnas 200a and 206 elevated in patients with refractory veno occlusive erectile dysfunction a comparative study
Urology, 2017Co-Authors: Sameh Fayek Gamalel Din, Laila A Rashed, Hany Ahmed Fouad Alghobary, Lamiaa Tarek Tawfik, Mohammed S ElsheemyAbstract:OBJECTIVE To evaluate the association between miRNAs and veno-occlusive erectile dysfunction. Recently, this association between miRNAs and erectile dysfunction was extensively studied using animal models. Our aim was to explore the miRNAs expressions and functions in the development of erectile dysfunction, especially veno-occlusive dysfunction, using a human Tissue. PATIENTS AND METHODS We prospectively recruited 60 patients with erectile dysfunction and controls between July 2015 and July 2016. The 30 patients had refractory veno-occlusive erectile dysfunction that was proven by investigations. They were scheduled for penile implant. The 30 controls were scheduled for repair of their fracture. We measured miRNAs (200a and 206) and nitric oxide in Cavernous Tissue and serum of both patients with erectile dysfunction and controls. RESULTS A significant association was found between the 2 mentioned miRNAs and erectile dysfunction (P <.001). Mean level of nitric oxide in Cavernous Tissue of the controls was significantly higher than that in the patients (P <.001). miRNA 200a showed a cutoff value of 1.135 with 95% sensitivity and 100% specificity, whereas miRNA 206 showed a cutoff value of 1.125 with 100% sensitivity and 100% specificity. CONCLUSION To the best of our knowledge, our study is the first report to measure the level of miRNAs in the Cavernous Tissue, using a human Tissue. Furthermore, this study can be considered a good step of deploying miRNAs through a blood test to detect early negative changes that lead to erectile dysfunction. Finally, we recommend more studies to be conducted to better understand if these miRNAs are involved in the pathophysiology of veno-occlusive erectile dysfunction.
-
Are the Cavernous Tissue and Serum Levels of Micro RNAs 200a and 206 Elevated in Patients With Refractory Veno-occlusive Erectile Dysfunction? A Comparative Study.
Urology, 2017Co-Authors: Sameh Fayek Gamalel Din, Laila A Rashed, Hany Ahmed Fouad Alghobary, Lamiaa Tarek Tawfik, Mohammed S ElsheemyAbstract:OBJECTIVE To evaluate the association between miRNAs and veno-occlusive erectile dysfunction. Recently, this association between miRNAs and erectile dysfunction was extensively studied using animal models. Our aim was to explore the miRNAs expressions and functions in the development of erectile dysfunction, especially veno-occlusive dysfunction, using a human Tissue. PATIENTS AND METHODS We prospectively recruited 60 patients with erectile dysfunction and controls between July 2015 and July 2016. The 30 patients had refractory veno-occlusive erectile dysfunction that was proven by investigations. They were scheduled for penile implant. The 30 controls were scheduled for repair of their fracture. We measured miRNAs (200a and 206) and nitric oxide in Cavernous Tissue and serum of both patients with erectile dysfunction and controls. RESULTS A significant association was found between the 2 mentioned miRNAs and erectile dysfunction (P
-
The role of PDE5 inhibitors in heme oxygenase-cGMP relationship in rat Cavernous Tissues.
The Journal of Sexual Medicine, 2008Co-Authors: M. T. Abdel Aziz, Taymour Mostafa, Hazem Atta, Samar Marzouk, Eman Obaia, Dina Sabry, Amira Hassouna, Amal El-shehaby, Laila A Rashed, Ahmed T. Abdel AzizAbstract:ABSTRACT Introduction Heme oxygenase (HO) enzyme catalyzes oxidative degradation of heme to biliverdin and carbon monoxide (CO). CO shares many properties with nitric oxide (NO) including the activation of soluble guanyl cyclase. Aim To assess Cavernous Tissue HO activity and cyclic guanosine monophosphate (cGMP) levels in response to oral phosphodiesterse type 5 (PDE5) inhibitors. Methods Seven hundred twenty male Sprague-Dawley rats, divided into six groups, were investigated. Group 1, controls; group 2 received sildenafil citrate orally; group 3 received vardenafil hydrochloride; and group 4 received tadalafil. Group 5 was subdivided into three equal subgroups, received the same dose of each drug added to the HO inhibitor, Zn protoporphyrin. Group 6 was subdivided into three equal subgroups, received the same dose of each drug added to the NO inhibitor, L-nitroarginine methylester. Eight rats from each group/subgroup were sacrificed at 0.5, 1, 2, 3, 4, 6, 18, 24, and 36 hours, respectively. Main Outcome Measures HO enzyme activity assay and cGMP Tissue levels in dissected rat Cavernous Tissues. Results Both Cavernous Tissue HO enzyme activity and cGMP levels were increased significantly in sildenafil-, vardenafil-, and tadalafil-treated rats compared with the controls, with significant decreases after either HO or NO inhibition. Cavernous Tissue HO enzyme activity and cGMP showed a positive significant correlation ( r = 0.854, P Conclusion The effects of PDE5 inhibitors in Cavernous Tissue are partly mediated through HO enzyme activity. Abdel Aziz MT, Mostafa T, Atta H, Rashed L, Marzouk SA, Obaia EM, Sabry D, Hassouna AA, El-Shehaby AM, and Abdel Aziz AT. The role of PDE5 inhibitors in heme oxygenase–cGMP relationship in rat Cavernous Tissues.
-
Assessment of heme oxygenase-1 (HO-1) activity in the Cavernous Tissues of sildenafil citrate-treated rats
Asian journal of andrology, 2007Co-Authors: M. T. Abdel Aziz, Taymour Mostafa, Hazem Atta, Dina Sabry, Laila A Rashed, M. Farid Al-asmar, Shedeed Ashour, Ahmed T. Abdel AzizAbstract:Aim: To assess heme oxygenase-1 (HO-1) activity in the Cavernous Tissue of sildenafil citrate-treated rats. Methods: One hundred and ninety-two Sprague-Dawley male rats, divided into four equal groups, were investigated. Group 1, the control group, received regular animal chow; group 2 received sildenafil citrate by intragastric tube; group 3 received sildenafil and HO inhibitor (zinc protoporphyrin, ZnPP); and group 4 received sildenafil and nitric oxide synthase (NOS) inhibitor L-nitroarginine methyl ester (L-NAME). Twelve rats from each group were killed after 0.5 h, 1 h, 2 h and 3 h of drug administration. Then HO-1 activity, cGMP levels and NOS enzymatic activity in the Cavernous Tissues were estimated. Results: In Cavernous Tissue, HO-1 activity, NOS enzymatic activity and cGMP concentration increased significantly in sildenafil-treated rats compared to other groups throughout the experiment. Rats receiving either HO or NOS inhibitors showed a significant decrease in these parameters. HO-1 Cavernous Tissue activity and NOS enzymatic activity demonstrated a positive significant correlation with cGMP levels (r = 0.646, r = 0.612 respectively; P < 0.001). Conclusion: The actions of PDE5 inhibitor sildenafil citrate in the Cavernous Tissue are partly mediated through the interdependent relationship between both HO-1 and NOS activities. (Asian J Androl 2007 May; 9: 377–381)
Vittorio Cavallari - One of the best experts on this subject based on the ideXlab platform.
-
Ultrastructural Study of Clitoral Cavernous Tissue and Clitoral Blood Flow From Type 1 Diabetic Premenopausal Women on Phosphodiesterase-5 Inhibitor.
The journal of sexual medicine, 2019Co-Authors: Salvatore Caruso, Antonio Cianci, Stefano Cianci, Caterina Monaco, V. Fava, Vittorio CavallariAbstract:Abstract Background The effects of phosphodiesterase-type 5 (PDE5) inhibitors on the in vivo clitoral structure of women with diabetes have never been investigated. Aim To study the in vivo structural and hemodynamic changes of the clitoris in premenopausal women with type 1 diabetes on PDE5 inhibitors. Methods 38 premenopausal women with type 1 diabetes aged 36 -46 years. A randomized 1:1 study design was used: Study Group (group A) on Tadalafil 5 mg daily, and control group (group B). Blood samples were taken from each woman to measure HbA1c, testosterone, and Free Androgen Index. The women underwent microbiopsy of the clitoral body by means of semiautomatic gun during total anesthesia for surgery therapy of a benign gynecological pathology. The Tissue removed was processed for electron microscopy. Translabial color Doppler ultrasound was used to measure the peak systolic velocity (PSV), the end diastolic velocity (EDV), and the pulsatility index (PI) of clitoral arteries. Main Outcome Measures Micro-ultrastructure observation of clitoral Tissue and color Doppler sonography of clitoral blood flow. Results Of the 38 women, 13 (68.4%) of group A and 15 (78.9%) of group B completed the study. Group A showed a mean PSV and EDV increase, and a mean PI decrease with respect to baseline (P Clinical Implications PDE5 inhibitors could be used to treat diabetic women with genital arousal disorder. Strengths & Limitations The study shows a clear effect of PDE5 inhibitors on clitoral SMCs. However, a limit was to not have investigated the sexual function/behavior of women of both groups, this was because of the short time of the study. Conclusion This study could help to understand in what way PDE5 inhibitors act on the ultrastructural pathophysiological clitoral Cavernous Tissue of women with diabetes. It could support PDE5 inhibitor usage in women with genital sexual arousal disorder due to metabolic diseases. Caruso S, Cianci A, Cianci S, et al. Ultrastructural Study of Clitoral Cavernous Tissue and Clitoral Blood Flow From Type 1 Diabetic Premenopausal Women on Phosphodiesterase-5 Inhibitor. J Sex Med 2019;16:375–382.
-
Ultrastructural and quantitative study of clitoral Cavernous Tissue from living subjects.
The journal of sexual medicine, 2011Co-Authors: Salvatore Caruso, Antonio Cianci, Chiara Malandrino, Lidia Cavallari, Orazio Gambadoro, Grazia Arena, Letterio Pispisa, Carmela Agnello, Mattea Romano, Vittorio CavallariAbstract:ABSTRACT Introduction There has never been an investigation about the in vivo clitoral structure. Aim To study the “in vivo” age-related structural changes of the clitoris in healthy women and in those affected by metabolic disorders. Methods Forty-three women subgrouping in (i) five teenagers, aged 14–18; (ii) eight young premenopausal women, aged 23–32; (iii) 10 premenopausal women, aged 38–47; (iv) nine diabetic premenopausal women, aged 27–43; and (v) 11 naturally postmenopausal women aged 51–55. Each woman underwent microbiopsy of the clitoral body by means of an 18G needle, length 100 mm, using a semiautomatic gun during total anesthesia for a benign gynecological pathology. The Tissue removed was processed for electron microscopy. A morphometric procedure was used on electron micrographs. Main Outcome Measure Micro ultrastructure observation of clitoral Tissue. Results The Cavernous Tissue from the teenagers and young women showed large amounts of smooth muscle cells (SMCs). The intercellular connective Tissue showed scanty, small isometric collagen fibers and amorphous extracellular matrix. In the premenopausal diabetic women, ultrastructural abnormalities of SMCs were observed, consisting of increase of glycogen deposits, infolding cell borders, and cytoplasmic vacuoles. Moreover, the intercellular connective Tissue was increased by densely packed collagen fibers. Finally, in the healthy, natural postmenopausal women, the SMCs were moderately reduced in number. We observed age-related structural changes of the vascular spaces and of the vascular lacunae. The SMC mean thickness was reduced with age; vascular abnormalities appeared to be correlated with the presence of metabolic diseases, such as diabetes. Conclusion Our “in vivo” study could help to understand some aspects of the physiology of the clitoris and its role in sexual response. Apart from data obtained by studying healthy women and women affected by diabetes, other investigations are needed to study subgroups of otherwise healthy sexually dysfunctional women. Caruso S, Cianci A, Malandrino C, Cavallari L, Gambadoro O, Arena G, Pispisa L, Agnello C, Romano M, and Cavallari V. Ultrastructural and quantitative study of clitoral Cavernous Tissue from living subjects.