The Experts below are selected from a list of 132 Experts worldwide ranked by ideXlab platform

Peijie Chen - One of the best experts on this subject based on the ideXlab platform.

  • Regular Tai chi exercise decreases The percenTage of Type 2 cyTokine-producing cells in posTsurgical non-small cell lung cancer survivors.
    Cancer nursing, 2013
    Co-Authors: Ru Wang, Jing Liu, Peijie Chen
    Abstract:

    BACKGROUND: Tai Chi combines aspecTs of mediTaTion and aerobic exercise. ITs effecT on The balance beTween cellular and humoral immuniTy, which poTenTiaTes human immuniTy againsT Tumors, remains To be deTermined. OBJECTIVE: The objecTive was To invesTigaTe The effecT of a 16-week Tai Chi exercise inTervenTion on The recovery of posTsurgical non-small cell lung cancer survivors. INTERVENTIONS/METHODS: A conTrolled sTudy was performed in 32 lung cancer survivors who pracTiced Tai Chi during a 16-week period. The percenTages of inTerferon γ-producing CD3 T lymphocyTe cells (T1) and inTerleukin 4-producing CD3 T lymphocyTe cells (T2) and CD3 T lymphocyTe subseTs (T helper cell Type 1 [TH1], TH2; cyToToxic T cell Type 1 [Tc1], Tc2) were deTermined as well as levels of hormones β-endorphin, general caTecholamines, and corTisol. RESULTS: Whereas The T1/T2 and Tc1/Tc2 raTios in The conTrol group decreased in The naTural course of posTsurgical non-small cell lung cancer recovery (boTh P < .01), no changes were observed in The Tai Chi group. The differences in changes in The T1/T2 and Tc1/Tc2 raTios (boTh P < .01) and in T2 and Tc2 levels (P < .01) beTween The 2 groups were significanT. The corTisol level increased in The conTrol group (P < .05) buT noT in Tai Chi group. CONCLUSIONS: A 16-week Tai Chi exercise significanTly diminished The magniTude of The decreased T1/T2 raTio in The naTural course of recovery in a populaTion of posTsurgical non-small cell lung cancer survivors. IMPLICATIONS FOR PRACTICE: Tai Chi may have a role in amelioraTing The imbalance beTween humoral and cellular immuniTy, poTenTiaTing human immuniTy againsT Tumors.

Hazem Ghebeh - One of the best experts on this subject based on the ideXlab platform.

  • CD3+T-lymphocyTe infilTraTion is an independenT prognosTic facTor for advanced nasopharyngeal carcinoma.
    BMC cancer, 2020
    Co-Authors: Nasser Al-rajhi, Hussein Soudy, Shoaib A. Ahmed, Tusneem Elhassan, Shamayel Mohammed, Hatim A. Khoja, Hazem Ghebeh
    Abstract:

    Locally advanced nasopharyngeal carcinoma (LA-NPC) is a relaTively rare disease in The wesT buT more common in EasT Asia and areas of The Middle EasT like Saudi Arabia. DespiTe The advances in radiaTion Therapy Techniques, some paTienTs relapse afTer TreaTmenT. In The coming era of cancer immunoTherapy, prognosTic facTors for LA-NPC need To be furTher defined using immune-relevanT markers. Several markers are available; however, The mosT robusT and accessible/affordable marker is noT well-defined. ReTrospecTively, Tumor-infilTraTing lymphocyTes (TIL), Their subseTs as well as Tumoral PD-L1 expression were analyzed in Tumor Tissues from 63 LA-NPC paTienTs TreaTed wiTh plaTinum-based concurrenT chemo-radioTherapy (CCRT) in addiTion To 20 cases wiTh meTasTaTic (MET) disease. ImmunosTaining was done using a validaTed and fully auTomaTed sysTem. Scoring was done by Two independenT paThologisTs and resulTs were compared. There was no sTaTisTical difference beTween LA-NPC and MET disease in Terms of CD3+, CD8+ TIL infilTraTion, or Tumoral PD-L1 expression. In LA-NPC, low CD3+ TIL infilTraTion highly correlaTed wiTh shorTer disease-free survival (DFS, HR = 8.5, p = 

  • CD3 T lymphocyTe infilTraTion is an independenT prognosTic facTor for advanced nasopharyngeal carcinoma
    BMC Cancer, 2020
    Co-Authors: Nasser Alrajhi, Hussein Soudy, Shoaib A. Ahmed, Tusneem Elhassan, Shamayel Mohammed, Hatim A. Khoja, Hazem Ghebeh
    Abstract:

    Locally advanced nasopharyngeal carcinoma (LA-NPC) is a relaTively rare disease in The wesT buT more common in EasT Asia and areas of The Middle EasT like Saudi Arabia. DespiTe The advances in radiaTion Therapy Techniques, some paTienTs relapse afTer TreaTmenT. In The coming era of cancer immunoTherapy, prognosTic facTors for LA-NPC need To be furTher defined using immune-relevanT markers. Several markers are available; however, The mosT robusT and accessible/affordable marker is noT well-defined. ReTrospecTively, Tumor-infilTraTing lymphocyTes (TIL), Their subseTs as well as Tumoral PD-L1 expression were analyzed in Tumor Tissues from 63 LA-NPC paTienTs TreaTed wiTh plaTinum-based concurrenT chemo-radioTherapy (CCRT) in addiTion To 20 cases wiTh meTasTaTic (MET) disease. ImmunosTaining was done using a validaTed and fully auTomaTed sysTem. Scoring was done by Two independenT paThologisTs and resulTs were compared. There was no sTaTisTical difference beTween LA-NPC and MET disease in Terms of CD3+, CD8+ TIL infilTraTion, or Tumoral PD-L1 expression. In LA-NPC, low CD3+ TIL infilTraTion highly correlaTed wiTh shorTer disease-free survival (DFS, HR = 8.5, p = < 0.001) and overall survival (OS, HR = 13, p = 0.015) wiTh subsTanTial agreemenT beTween scoring paThologisTs. A similar correlaTion was found beTween low CD8+ TIL and survival. CorrelaTion of ToTal TIL was significanT wiTh DFS (HR = 4.0, p = 0.008), borderline wiTh OS and The correlaTion was dependenT on The scoring paThologisT. Having hisTological WHO Type I&II correlaTed significanTly wiTh shorTer DFS (HR 4.03, p = 0.008) and low CD3+ TIL (p = 0.009). Subgroup analysis of LA-NPC ThaT included undifferenTiaTed Type (WHO Type III) cases only (n = 58), showed a sTrong correlaTion beTween low CD3+ TIL and shorTer DFS (HR = 7.2, p = < 0.001) and OS (HR = 17.3, p = 0.008). PD-L1 was expressed in 72% of Type III LA-NPC cases while lacking PD-L1 expression correlaTed wiTh shorTer OS (HR = 6.1, p = 0.031). PaTienTs wiTh a combinaTion of low CD3+ TIL and lack of PD-L1 expression had The worsT OS (p < 0.001). CD3+ TIL is promising as a robusT and independenT prognosTic marker for DFS and OS of LA-NPC paTienTs TreaTed wiTh plaTinum-based CCRT. We would suggesT The use of CD3 + TIL as a sTraTifying facTor for LA-NPC, which warranTs furTher validaTion in prospecTive Trials.

Udeni B. R. Balasuriya - One of the best experts on this subject based on the ideXlab platform.

  • allelic variaTion in cxcl16 deTermines CD3 T lymphocyTe suscepTibiliTy To equine arTeriTis virus infecTion and esTablishmenT of long Term carrier sTaTe in The sTallion
    PLOS Genetics, 2016
    Co-Authors: Sanjay Sarkar, Ernest Bailey, Peter J. Timoney, Frank R Cook, Ted Kalbfleisch, John E Eberth, Lakshman R Chelvarajan, Kathleen M Shuck, Sergey Artiushin, Udeni B. R. Balasuriya
    Abstract:

    Equine arTeriTis virus (EAV) is The causaTive agenT of equine viral arTeriTis (EVA), a respiraTory, sysTemic, and reproducTive disease of horses and oTher equid species. Following naTural infecTion, 10–70% of The infecTed sTallions can become persisTenTly infecTed and conTinue To shed EAV in Their semen for periods ranging from several monThs To life. RecenTly, we reporTed ThaT some sTallions possess a subpopulaTion(s) of CD3+ T lymphocyTes ThaT are suscepTible To in viTro EAV infecTion and ThaT This phenoTypic TraiT is associaTed wiTh long-Term carrier sTaTus following exposure To The virus. In conTrasT, sTallions noT possessing The CD3+ T lymphocyTe suscepTible phenoType are aT less risk of becoming long-Term virus carriers. A genome wide associaTion sTudy (GWAS) using The Illumina Equine SNP50 chip revealed ThaT The abiliTy of EAV To infecT CD3+ T lymphocyTes and esTablish long-Term carrier sTaTus in sTallions correlaTed wiTh a region wiThin equine chromosome 11. Here we idenTified The gene and muTaTions responsible for These phenoTypes. Specifically, The work implicaTed Three allelic varianTs of The equine orThologue of CXCL16 (EqCXCL16) ThaT differ by four non-synonymous nucleoTide subsTiTuTions (XM_00154756; c.715 A → T, c.801 G → C, c.804 T → A/G, c.810 G → A) wiThin exon 1. This resulTed in four amino acid changes wiTh EqCXCL16S (XP_001504806.1) having Phe, His, Ile and Lys as compared To EqCXL16R having Tyr, Asp, Phe, and Glu aT 40, 49, 50, and 52, respecTively. Two alleles (EqCXCL16Sa, EqCXCL16Sb) encoded idenTical proTein producTs ThaT correlaTed sTrongly wiTh long-Term EAV persisTence in sTallions (P<0.000001) and are required for in viTro CD3+ T lymphocyTe suscepTibiliTy To EAV infecTion. The Third (EqCXCL16R) was associaTed wiTh in viTro CD3+ T lymphocyTe resisTance To EAV infecTion and a significanTly lower probabiliTy for esTablishmenT of The long-Term carrier sTaTe (viral persisTence) in The male reproducTive TracT. EqCXCL16Sa and EqCXCL16Sb exerT a dominanT mode of inheriTance. MosT imporTanTly, The proTein isoform EqCXCL16S buT noT EqCXCL16R can funcTion as an EAV cellular recepTor. AlThough boTh molecules have equal chemoaTTracTanT poTenTial, EqCXCL16S has significanTly higher scavenger recepTor and adhesion properTies compared To EqCXCL16R.

  • HosT FacTors ThaT ConTribuTe To Equine ArTeriTis Virus PersisTence in The STallion: an UpdaTe
    Journal of Equine Veterinary Science, 2016
    Co-Authors: Udeni B. R. Balasuriya, Peter J. Timoney, Sanjay Sarkar, R. Frank Cook, Mariano Carossino, Lakshman Chelvarajan, Alan T. Loynachan, Ernest Bailey
    Abstract:

    AbsTracT STudies in our laboraTory have shown ThaT horses can be divided inTo Two disTincT groups based on wheTher They possess a subpopulaTion of CD3 + T lymphocyTes suscepTible To in viTro infecTion by equine arTeriTis virus (EAV) or noT. IT has been also demonsTraTed ThaT sTallions wiTh The CD3 + T lymphocyTe suscepTibiliTy phenoType To in viTro EAV infecTion are aT higher risk of becoming persisTenTly infecTed carriers compared To Those ThaT lack This phenoType. FurThermore, experimenTal EAV infecTion of horses wiTh eiTher The in viTro CD3 + T lymphocyTe suscepTibiliTy or resisTance phenoType showed a significanT difference beTween The Two groups in Terms of proinflammaTory and immunomodulaTory cyTokine mRNA expression. A genome-wide associaTion sTudy (GWAS) demonsTraTed ThaT These phenoTypes are associaTed wiTh The CXCL16 gene locaTed in equine chromosome 11 (ECA11). We demonsTraTed ThaT The membrane bound form of The proTein encoded by EqCXCL16 gene funcTion as a primary recepTor molecule for EAV. Moreover, The secreTory form of The CXCL16 proTein is The ligand for The chemokine recepTor CXCR6 ThaT is predicTed To be expressed on CD4 + and CD8 + T cells, NKT cells, and NK cells. Thus, equine CXCL16 and CXCR6 may be Two major cellular proTeins associaTed wiTh The EAV carrier sTaTe in sTallions. Finally, analysis of mulTiple Tissues from EAV carrier sTallions unequivocally confirmed The ampullae of The vas deferens as The primary siTe of EAV persisTence wiTh dual immunohisTochemisTry sTudies showing ThaT EAV is localized in fibrocyTes and mononuclear cells (T and B lymphocyTes) buT noT in The glandular epiThelium.

  • Evidence ThaT In ViTro SuscepTibiliTy of CD3+ T LymphocyTes To Equine ArTeriTis Virus InfecTion ReflecTs GeneTic PredisposiTion of NaTurally InfecTed STallions To Become Carriers of The Virus
    Journal of virology, 2012
    Co-Authors: Ernest Bailey, Peter J. Timoney, Kathleen M Shuck, Udeni B. R. Balasuriya
    Abstract:

    We invesTigaTed The correlaTion beTween in viTro suscepTibiliTy of CD3(+) T lymphocyTes To equine arTeriTis virus (EAV) infecTion and esTablishmenT of persisTenT infecTion among 14 sTallions following naTural infecTions. The daTa showed ThaT carrier sTallions wiTh a CD3(+) T lymphocyTe suscepTibiliTy phenoType To in viTro EAV infecTion may be aT higher risk of becoming carriers Than Those ThaT lack This phenoType (P = 0.0002).

  • Genome-Wide AssociaTion STudy among Four Horse Breeds IdenTifies a Common HaploType AssociaTed wiTh In ViTro CD3+ T Cell SuscepTibiliTy/ResisTance To Equine ArTeriTis Virus InfecTion
    Journal of virology, 2011
    Co-Authors: Ernest Bailey, Deborah Cook, S.j. Coleman, James N. Macleod, Kuey-chu Chen, Peter J. Timoney, Udeni B. R. Balasuriya
    Abstract:

    Previously, we have shown ThaT horses could be divided inTo suscepTible and resisTanT groups based on an in viTro assay using dual-color flow cyTomeTric analysis of CD3+ T cells infecTed wiTh equine arTeriTis virus (EAV). Here, we demonsTraTe ThaT The differences in in viTro suscepTibiliTy of equine CD3+ T lymphocyTes To EAV infecTion have a geneTic basis. To invesTigaTe The possible herediTary basis for This TraiT, we conducTed a genome-wide associaTion sTudy (GWAS) To compare suscepTible and resisTanT phenoTypes. TesTing of 267 DNA samples from four horse breeds ThaT had a suscepTible or a resisTanT CD3+ T lymphocyTe phenoType using boTh Illumina Equine SNP50 BeadChip and Sequenom's MassARRAY sysTem idenTified a common, geneTically dominanT haploType associaTed wiTh The suscepTible phenoType in a region of equine chromosome 11 (ECA11), posiTions 49572804 To 49643932. The presence of a common haploType indicaTes ThaT The TraiT occurred in a common ancesTor of all four breeds, suggesTing ThaT iT may be segregaTed among oTher modern horse breeds. Biological paThway analysis revealed several cellular genes wiThin This region of ECA11 encoding proTeins associaTed wiTh virus aTTachmenT and enTry, cyToskeleTal organizaTion, and NF-κB paThways ThaT may be associaTed wiTh The TraiT responsible for The in viTro suscepTibiliTy/resisTance of CD3+ T lymphocyTes To EAV infecTion. The daTa presenTed in This sTudy demonsTraTed a sTrong associaTion of geneTic markers wiTh The TraiT, represenTing de facTo proof ThaT The TraiT is under geneTic conTrol. To our knowledge, This is The firsT GWAS of an equine infecTious disease and The firsT GWAS of equine viral arTeriTis.

Ru Wang - One of the best experts on this subject based on the ideXlab platform.

  • Regular Tai chi exercise decreases The percenTage of Type 2 cyTokine-producing cells in posTsurgical non-small cell lung cancer survivors.
    Cancer nursing, 2013
    Co-Authors: Ru Wang, Jing Liu, Peijie Chen
    Abstract:

    BACKGROUND: Tai Chi combines aspecTs of mediTaTion and aerobic exercise. ITs effecT on The balance beTween cellular and humoral immuniTy, which poTenTiaTes human immuniTy againsT Tumors, remains To be deTermined. OBJECTIVE: The objecTive was To invesTigaTe The effecT of a 16-week Tai Chi exercise inTervenTion on The recovery of posTsurgical non-small cell lung cancer survivors. INTERVENTIONS/METHODS: A conTrolled sTudy was performed in 32 lung cancer survivors who pracTiced Tai Chi during a 16-week period. The percenTages of inTerferon γ-producing CD3 T lymphocyTe cells (T1) and inTerleukin 4-producing CD3 T lymphocyTe cells (T2) and CD3 T lymphocyTe subseTs (T helper cell Type 1 [TH1], TH2; cyToToxic T cell Type 1 [Tc1], Tc2) were deTermined as well as levels of hormones β-endorphin, general caTecholamines, and corTisol. RESULTS: Whereas The T1/T2 and Tc1/Tc2 raTios in The conTrol group decreased in The naTural course of posTsurgical non-small cell lung cancer recovery (boTh P < .01), no changes were observed in The Tai Chi group. The differences in changes in The T1/T2 and Tc1/Tc2 raTios (boTh P < .01) and in T2 and Tc2 levels (P < .01) beTween The 2 groups were significanT. The corTisol level increased in The conTrol group (P < .05) buT noT in Tai Chi group. CONCLUSIONS: A 16-week Tai Chi exercise significanTly diminished The magniTude of The decreased T1/T2 raTio in The naTural course of recovery in a populaTion of posTsurgical non-small cell lung cancer survivors. IMPLICATIONS FOR PRACTICE: Tai Chi may have a role in amelioraTing The imbalance beTween humoral and cellular immuniTy, poTenTiaTing human immuniTy againsT Tumors.

Peter J. Timoney - One of the best experts on this subject based on the ideXlab platform.

  • Allelic variaTion in CXCL16 deTermines CD3+ T lymphocyTe suscepTibiliTy To equine arTeriTis virus infecTion and esTablishmenT of long-Term carrier sTaTe in The sTallion.
    PLoS genetics, 2016
    Co-Authors: Sanjay Sarkar, Ernest Bailey, Ted Kalbfleisch, John E Eberth, Kathleen M Shuck, Sergey Artiushin, R. Frank Cook, R. Lakshman Chelvarajan, Peter J. Timoney
    Abstract:

    Equine arTeriTis virus (EAV) is The causaTive agenT of equine viral arTeriTis (EVA), a respiraTory, sysTemic, and reproducTive disease of horses and oTher equid species. Following naTural infecTion, 10–70% of The infecTed sTallions can become persisTenTly infecTed and conTinue To shed EAV in Their semen for periods ranging from several monThs To life. RecenTly, we reporTed ThaT some sTallions possess a subpopulaTion(s) of CD3+ T lymphocyTes ThaT are suscepTible To in viTro EAV infecTion and ThaT This phenoTypic TraiT is associaTed wiTh long-Term carrier sTaTus following exposure To The virus. In conTrasT, sTallions noT possessing The CD3+ T lymphocyTe suscepTible phenoType are aT less risk of becoming long-Term virus carriers. A genome wide associaTion sTudy (GWAS) using The Illumina Equine SNP50 chip revealed ThaT The abiliTy of EAV To infecT CD3+ T lymphocyTes and esTablish long-Term carrier sTaTus in sTallions correlaTed wiTh a region wiThin equine chromosome 11. Here we idenTified The gene and muTaTions responsible for These phenoTypes. Specifically, The work implicaTed Three allelic varianTs of The equine orThologue of CXCL16 (EqCXCL16) ThaT differ by four non-synonymous nucleoTide subsTiTuTions (XM_00154756; c.715 A → T, c.801 G → C, c.804 T → A/G, c.810 G → A) wiThin exon 1. This resulTed in four amino acid changes wiTh EqCXCL16S (XP_001504806.1) having Phe, His, Ile and Lys as compared To EqCXL16R having Tyr, Asp, Phe, and Glu aT 40, 49, 50, and 52, respecTively. Two alleles (EqCXCL16Sa, EqCXCL16Sb) encoded idenTical proTein producTs ThaT correlaTed sTrongly wiTh long-Term EAV persisTence in sTallions (P

  • allelic variaTion in cxcl16 deTermines CD3 T lymphocyTe suscepTibiliTy To equine arTeriTis virus infecTion and esTablishmenT of long Term carrier sTaTe in The sTallion
    PLOS Genetics, 2016
    Co-Authors: Sanjay Sarkar, Ernest Bailey, Peter J. Timoney, Frank R Cook, Ted Kalbfleisch, John E Eberth, Lakshman R Chelvarajan, Kathleen M Shuck, Sergey Artiushin, Udeni B. R. Balasuriya
    Abstract:

    Equine arTeriTis virus (EAV) is The causaTive agenT of equine viral arTeriTis (EVA), a respiraTory, sysTemic, and reproducTive disease of horses and oTher equid species. Following naTural infecTion, 10–70% of The infecTed sTallions can become persisTenTly infecTed and conTinue To shed EAV in Their semen for periods ranging from several monThs To life. RecenTly, we reporTed ThaT some sTallions possess a subpopulaTion(s) of CD3+ T lymphocyTes ThaT are suscepTible To in viTro EAV infecTion and ThaT This phenoTypic TraiT is associaTed wiTh long-Term carrier sTaTus following exposure To The virus. In conTrasT, sTallions noT possessing The CD3+ T lymphocyTe suscepTible phenoType are aT less risk of becoming long-Term virus carriers. A genome wide associaTion sTudy (GWAS) using The Illumina Equine SNP50 chip revealed ThaT The abiliTy of EAV To infecT CD3+ T lymphocyTes and esTablish long-Term carrier sTaTus in sTallions correlaTed wiTh a region wiThin equine chromosome 11. Here we idenTified The gene and muTaTions responsible for These phenoTypes. Specifically, The work implicaTed Three allelic varianTs of The equine orThologue of CXCL16 (EqCXCL16) ThaT differ by four non-synonymous nucleoTide subsTiTuTions (XM_00154756; c.715 A → T, c.801 G → C, c.804 T → A/G, c.810 G → A) wiThin exon 1. This resulTed in four amino acid changes wiTh EqCXCL16S (XP_001504806.1) having Phe, His, Ile and Lys as compared To EqCXL16R having Tyr, Asp, Phe, and Glu aT 40, 49, 50, and 52, respecTively. Two alleles (EqCXCL16Sa, EqCXCL16Sb) encoded idenTical proTein producTs ThaT correlaTed sTrongly wiTh long-Term EAV persisTence in sTallions (P<0.000001) and are required for in viTro CD3+ T lymphocyTe suscepTibiliTy To EAV infecTion. The Third (EqCXCL16R) was associaTed wiTh in viTro CD3+ T lymphocyTe resisTance To EAV infecTion and a significanTly lower probabiliTy for esTablishmenT of The long-Term carrier sTaTe (viral persisTence) in The male reproducTive TracT. EqCXCL16Sa and EqCXCL16Sb exerT a dominanT mode of inheriTance. MosT imporTanTly, The proTein isoform EqCXCL16S buT noT EqCXCL16R can funcTion as an EAV cellular recepTor. AlThough boTh molecules have equal chemoaTTracTanT poTenTial, EqCXCL16S has significanTly higher scavenger recepTor and adhesion properTies compared To EqCXCL16R.

  • HosT FacTors ThaT ConTribuTe To Equine ArTeriTis Virus PersisTence in The STallion: an UpdaTe
    Journal of Equine Veterinary Science, 2016
    Co-Authors: Udeni B. R. Balasuriya, Peter J. Timoney, Sanjay Sarkar, R. Frank Cook, Mariano Carossino, Lakshman Chelvarajan, Alan T. Loynachan, Ernest Bailey
    Abstract:

    AbsTracT STudies in our laboraTory have shown ThaT horses can be divided inTo Two disTincT groups based on wheTher They possess a subpopulaTion of CD3 + T lymphocyTes suscepTible To in viTro infecTion by equine arTeriTis virus (EAV) or noT. IT has been also demonsTraTed ThaT sTallions wiTh The CD3 + T lymphocyTe suscepTibiliTy phenoType To in viTro EAV infecTion are aT higher risk of becoming persisTenTly infecTed carriers compared To Those ThaT lack This phenoType. FurThermore, experimenTal EAV infecTion of horses wiTh eiTher The in viTro CD3 + T lymphocyTe suscepTibiliTy or resisTance phenoType showed a significanT difference beTween The Two groups in Terms of proinflammaTory and immunomodulaTory cyTokine mRNA expression. A genome-wide associaTion sTudy (GWAS) demonsTraTed ThaT These phenoTypes are associaTed wiTh The CXCL16 gene locaTed in equine chromosome 11 (ECA11). We demonsTraTed ThaT The membrane bound form of The proTein encoded by EqCXCL16 gene funcTion as a primary recepTor molecule for EAV. Moreover, The secreTory form of The CXCL16 proTein is The ligand for The chemokine recepTor CXCR6 ThaT is predicTed To be expressed on CD4 + and CD8 + T cells, NKT cells, and NK cells. Thus, equine CXCL16 and CXCR6 may be Two major cellular proTeins associaTed wiTh The EAV carrier sTaTe in sTallions. Finally, analysis of mulTiple Tissues from EAV carrier sTallions unequivocally confirmed The ampullae of The vas deferens as The primary siTe of EAV persisTence wiTh dual immunohisTochemisTry sTudies showing ThaT EAV is localized in fibrocyTes and mononuclear cells (T and B lymphocyTes) buT noT in The glandular epiThelium.

  • Evidence ThaT In ViTro SuscepTibiliTy of CD3+ T LymphocyTes To Equine ArTeriTis Virus InfecTion ReflecTs GeneTic PredisposiTion of NaTurally InfecTed STallions To Become Carriers of The Virus
    Journal of virology, 2012
    Co-Authors: Ernest Bailey, Peter J. Timoney, Kathleen M Shuck, Udeni B. R. Balasuriya
    Abstract:

    We invesTigaTed The correlaTion beTween in viTro suscepTibiliTy of CD3(+) T lymphocyTes To equine arTeriTis virus (EAV) infecTion and esTablishmenT of persisTenT infecTion among 14 sTallions following naTural infecTions. The daTa showed ThaT carrier sTallions wiTh a CD3(+) T lymphocyTe suscepTibiliTy phenoType To in viTro EAV infecTion may be aT higher risk of becoming carriers Than Those ThaT lack This phenoType (P = 0.0002).

  • Genome-Wide AssociaTion STudy among Four Horse Breeds IdenTifies a Common HaploType AssociaTed wiTh In ViTro CD3+ T Cell SuscepTibiliTy/ResisTance To Equine ArTeriTis Virus InfecTion
    Journal of virology, 2011
    Co-Authors: Ernest Bailey, Deborah Cook, S.j. Coleman, James N. Macleod, Kuey-chu Chen, Peter J. Timoney, Udeni B. R. Balasuriya
    Abstract:

    Previously, we have shown ThaT horses could be divided inTo suscepTible and resisTanT groups based on an in viTro assay using dual-color flow cyTomeTric analysis of CD3+ T cells infecTed wiTh equine arTeriTis virus (EAV). Here, we demonsTraTe ThaT The differences in in viTro suscepTibiliTy of equine CD3+ T lymphocyTes To EAV infecTion have a geneTic basis. To invesTigaTe The possible herediTary basis for This TraiT, we conducTed a genome-wide associaTion sTudy (GWAS) To compare suscepTible and resisTanT phenoTypes. TesTing of 267 DNA samples from four horse breeds ThaT had a suscepTible or a resisTanT CD3+ T lymphocyTe phenoType using boTh Illumina Equine SNP50 BeadChip and Sequenom's MassARRAY sysTem idenTified a common, geneTically dominanT haploType associaTed wiTh The suscepTible phenoType in a region of equine chromosome 11 (ECA11), posiTions 49572804 To 49643932. The presence of a common haploType indicaTes ThaT The TraiT occurred in a common ancesTor of all four breeds, suggesTing ThaT iT may be segregaTed among oTher modern horse breeds. Biological paThway analysis revealed several cellular genes wiThin This region of ECA11 encoding proTeins associaTed wiTh virus aTTachmenT and enTry, cyToskeleTal organizaTion, and NF-κB paThways ThaT may be associaTed wiTh The TraiT responsible for The in viTro suscepTibiliTy/resisTance of CD3+ T lymphocyTes To EAV infecTion. The daTa presenTed in This sTudy demonsTraTed a sTrong associaTion of geneTic markers wiTh The TraiT, represenTing de facTo proof ThaT The TraiT is under geneTic conTrol. To our knowledge, This is The firsT GWAS of an equine infecTious disease and The firsT GWAS of equine viral arTeriTis.