The Experts below are selected from a list of 345 Experts worldwide ranked by ideXlab platform
Hengmin Cui - One of the best experts on this subject based on the ideXlab platform.
-
Effect of dietary NiCl2 on the cell cycle of Cecal Tonsil in the chicken broiler.
Anais da Academia Brasileira de Ciencias, 2020Co-Authors: Baolin Song, Hengmin CuiAbstract:Although the effects of nickel chloride (NiCl2) on the immune system have long been recognized, little is known about the effects of nickel (II) on the cell cycle and related signaling events in immune organs, such as Cecal Tonsil, a key immune organ of chicken. In the present study, we investigated the effect of NiCl2 on the cell cycle of Cecal Tonsil. The cell cycle was detected by the methods of flow cytometry (FCM), quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC). The results showed that dietary NiCl2 in excess of 300 mg/kg caused the G2/M cell cycle arrest and the reduction of cell proportion at S phase of the Cecal Tonsil. The G2/M cell cycle arrest was accompanied by the up-regulation of p53, p21 protein expression and mRNA expression, and down-regulation of cyclinB and proliferating cell nuclear antigen (PCNA) protein expression and mRNA expression. The data suggested that the cells' (mainly the T lymphocytes) proliferation in the Cecal Tonsil was inhibited by the high dietary NiCl2.
-
selenium rescues aflatoxin b 1 inhibited t cell subsets and cytokine levels in Cecal Tonsil of chickens
Biological Trace Element Research, 2019Co-Authors: Fengyuan Wang, Zhicai Zuo, Xi Peng, Jing Fang, Hengmin Cui, Kejie Chen, Gang Shu, Li TangAbstract:Cecal Tonsil is the largest peripheral lymphoid organ of the gut-associated lymphoid tissue executing immune function. To evaluate the protective effect of selenium (Se) on the Cecal Tonsil of chicken exposed to aflatoxin B1 (AFB1), 144 1-day-old healthy Cobb chickens were randomly divided into four groups, and fed with basal diet (control group), 0.6 mg/kg AFB1 (AFB1 group), 0.4 mg/kg Se supplement (+Se group), and 0.6 mg/kg AFB1 + 0.4 mg/kg Se supplement (AFB1 + Se group) for 21 days, respectively. The results showed that AFB1 significantly decreased the percentages of CD3+, CD3+CD4+, CD3+CD8+ T cells, and the CD4+/CD8+ ratio, and suppressed the expressions of IL-2, IL-4, TNF-α, and IFN-γ mRNA in the Cecal Tonsil. However, Selenium (Se) supplied in the diets restored the percentages of T cell subsets, the CD4+/CD8+ ratio, and mRNA expressions of cytokines in the AFB1 group to be close to those in the control group, and did not exhibit obvious toxicity to the Cecal Tonsil. These results indicated that Se exerted protective effect against AFB1 on the functions of Cecal Tonsil, and also partially uncovered a new role of Se that could protect Cecal Tonsil of chickens from immunotoxicity of AFB1.
-
sodium selenite prevents suppression of mucosal humoral response by afb 1 in broiler s Cecal Tonsil
Oncotarget, 2017Co-Authors: Chunyu Liu, Zhicai Zuo, Xi Peng, Jing Fang, Hengmin Cui, Panpan Zhu, Zhixiang Zheng, Yi Zhou, Ping Ouyang, Yi GengAbstract:// Chunyu Liu 1, 2, * , Zhicai Zuo 1, 2, * , Panpan Zhu 1, 2, * , Zhixiang Zheng 1, 2, * , Xi Peng 3 , Jing Fang 1, 2 , Hengmin Cui 1, 2 , Yi Zhou 4 , Ping Ouyang 2 , Yi Geng 2 , Junliang Deng 1, 2 and Yu Sun 2 1 Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China 2 College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China 3 College of Life Sciences, China West Normal University, Nanchong, Sichuan, PR China 4 Life Science Department, Sichuan Agricultural University, Yaan, Sichuan, PR China * These authors contributed equally to this work Correspondence to: Jing Fang, email: fangjing4109@163.com Xi Peng, email: pengxi197313@163.com Keywords: aflatoxin B 1 , selenium, IgA+ cell, immunoglobulin, Cecal Tonsil Received: February 06, 2017 Accepted: April 02, 2017 Published: April 13, 2017 ABSTRACT Aflatoxin B 1 (AFB 1 ), the most common mycotoxin in human food and animal feed, produces hepatotoxic, genotoxic and immunosuppressive effects in multiple species. Selenium (Se) has emerged as an important element in the dietary prevention of various toxic agents. The present study was designed to scrutinize the protective effects of sodium selenite on the histological lesions and suppression of mucosal humoral response in the Cecal Tonsil generated by AFB 1 . A total of 156 one-day-old broilers were divided into four groups and fed on basal diet (control group), 0.6 mg/kg AFB 1 (AFB 1 group), 0.4 mg/kg Se supplement (+Se group), and 0.6 mg/kg AFB 1 + 0.4 mg/kg Se supplement (AFB 1 +Se group) respectively for 21 days. Our results showed that 0.4 mg/kg Se supplement in broiler’s diets could improve the AFB 1 -induced histological lesions in the Cecal Tonsils including the depletion of lymphocytes in the lymphatic nodules as well as the shedding of microvilli in the absorptive cells. Moreover, Se could restore the decreased number of IgA+ cells and expression levels of pIgR, IgA, IgG, and IgM mRNA induced by AFB 1 to be close to those in the control group. These results demonstrated that 0.4 mg/kg supplemented dietary Se in the form of sodium selenite could protect the Cecal Tonsils from the histological lesions and suppression of the mucosal humoral response provoked by 0.6 mg/kg AFB 1 . Our study may provide new experimental evidences for better understanding of AFB 1 -induced damage of mucosal immunity and protective effect of Se against this toxin.
-
Short communication: Inhibitory effects of dietary aflatoxin B1 on cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens
Spanish Journal of Agricultural Research, 2016Co-Authors: Chunyu Liu, Xi Peng, Jing Fang, Min Jiang, Hengmin CuiAbstract:Aflatoxin B 1 (AFB 1 ) is the most toxic form among the mycotoxins. Cytokines are important mediators of the immune system. T-cell subsets play a crucial role in cell-mediated immunity. The aim of present study was to evaluate the effects of dietary AFB 1 on the cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens throughout a 21-day experimental period. One hundred and fifty six one-day-old broiler chickens were randomly divided into control group (0 mg AFB 1 /kg feed) and AFB 1 group (0.6 mg pure AFB 1 /kg feed). At 7, 14 and 21 days of age, the levels of seven cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α) mRNA expression as well as the proportions of T-cell subsets (CD3+, CD3+CD4+, CD3+CD8+) by qRT-PCR and flow cytometry methods were assessed in the Cecal Tonsils. The levels of the seven cytokines mRNA expression and the percentages of T-cell subsets significantly decreased at 14 and 21 days of age in the AFB 1 group compared with the control group. However, the CD4+/CD8+ ratio was not significantly changed. These results demonstrate that 0.6 mg/kg AFB 1 dietary exposure reduced the levels of cytokines mRNA expression and the percentages of T-cell subsets in the Cecal Tonsils of broiler chickens, suggesting that the cell-mediated immunity of Cecal Tonsils might be impaired in broilers.
-
Inhibitory effects of dietary aflatoxin B1 on cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens
Spanish Journal of Agricultural Research, 2016Co-Authors: Chunyu Liu, Xi Peng, Jing Fang, Min Jiang, Hengmin CuiAbstract:Aflatoxin B1 (AFB1) is the most toxic form among the mycotoxins. Cytokines are important mediators of the immune system. T-cell subsets play a crucial role in cell-mediated immunity. The aim of present study was to evaluate the effects of dietary AFB1 on the cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens throughout a 21-day experimental period. One hundred and fifty six one-day-old broiler chickens were randomly divided into control group (0 mg AFB1/kg feed) and AFB1 group (0.6 mg pure AFB1/kg feed). At 7, 14 and 21 days of age, the levels of seven cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α) mRNA expression as well as the proportions of T-cell subsets (CD3+, CD3+CD4+, CD3+CD8+) by qRT-PCR and flow cytometry methods were assessed in the Cecal Tonsils. The levels of the seven cytokines mRNA expression and the percentages of T-cell subsets significantly decreased at 14 and 21 days of age in the AFB1 group compared with the control group. However, the CD4+/CD8+ ratio was not significantly changed. These results demonstrate that 0.6 mg/kg AFB1 dietary exposure reduced the levels of cytokines mRNA expression and the percentages of T-cell subsets in the Cecal Tonsils of broiler chickens, suggesting that the cell-mediated immunity of Cecal Tonsils might be impaired in broilers.
Jing Fang - One of the best experts on this subject based on the ideXlab platform.
-
selenium rescues aflatoxin b 1 inhibited t cell subsets and cytokine levels in Cecal Tonsil of chickens
Biological Trace Element Research, 2019Co-Authors: Fengyuan Wang, Zhicai Zuo, Xi Peng, Jing Fang, Hengmin Cui, Kejie Chen, Gang Shu, Li TangAbstract:Cecal Tonsil is the largest peripheral lymphoid organ of the gut-associated lymphoid tissue executing immune function. To evaluate the protective effect of selenium (Se) on the Cecal Tonsil of chicken exposed to aflatoxin B1 (AFB1), 144 1-day-old healthy Cobb chickens were randomly divided into four groups, and fed with basal diet (control group), 0.6 mg/kg AFB1 (AFB1 group), 0.4 mg/kg Se supplement (+Se group), and 0.6 mg/kg AFB1 + 0.4 mg/kg Se supplement (AFB1 + Se group) for 21 days, respectively. The results showed that AFB1 significantly decreased the percentages of CD3+, CD3+CD4+, CD3+CD8+ T cells, and the CD4+/CD8+ ratio, and suppressed the expressions of IL-2, IL-4, TNF-α, and IFN-γ mRNA in the Cecal Tonsil. However, Selenium (Se) supplied in the diets restored the percentages of T cell subsets, the CD4+/CD8+ ratio, and mRNA expressions of cytokines in the AFB1 group to be close to those in the control group, and did not exhibit obvious toxicity to the Cecal Tonsil. These results indicated that Se exerted protective effect against AFB1 on the functions of Cecal Tonsil, and also partially uncovered a new role of Se that could protect Cecal Tonsil of chickens from immunotoxicity of AFB1.
-
sodium selenite prevents suppression of mucosal humoral response by afb 1 in broiler s Cecal Tonsil
Oncotarget, 2017Co-Authors: Chunyu Liu, Zhicai Zuo, Xi Peng, Jing Fang, Hengmin Cui, Panpan Zhu, Zhixiang Zheng, Yi Zhou, Ping Ouyang, Yi GengAbstract:// Chunyu Liu 1, 2, * , Zhicai Zuo 1, 2, * , Panpan Zhu 1, 2, * , Zhixiang Zheng 1, 2, * , Xi Peng 3 , Jing Fang 1, 2 , Hengmin Cui 1, 2 , Yi Zhou 4 , Ping Ouyang 2 , Yi Geng 2 , Junliang Deng 1, 2 and Yu Sun 2 1 Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China 2 College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China 3 College of Life Sciences, China West Normal University, Nanchong, Sichuan, PR China 4 Life Science Department, Sichuan Agricultural University, Yaan, Sichuan, PR China * These authors contributed equally to this work Correspondence to: Jing Fang, email: fangjing4109@163.com Xi Peng, email: pengxi197313@163.com Keywords: aflatoxin B 1 , selenium, IgA+ cell, immunoglobulin, Cecal Tonsil Received: February 06, 2017 Accepted: April 02, 2017 Published: April 13, 2017 ABSTRACT Aflatoxin B 1 (AFB 1 ), the most common mycotoxin in human food and animal feed, produces hepatotoxic, genotoxic and immunosuppressive effects in multiple species. Selenium (Se) has emerged as an important element in the dietary prevention of various toxic agents. The present study was designed to scrutinize the protective effects of sodium selenite on the histological lesions and suppression of mucosal humoral response in the Cecal Tonsil generated by AFB 1 . A total of 156 one-day-old broilers were divided into four groups and fed on basal diet (control group), 0.6 mg/kg AFB 1 (AFB 1 group), 0.4 mg/kg Se supplement (+Se group), and 0.6 mg/kg AFB 1 + 0.4 mg/kg Se supplement (AFB 1 +Se group) respectively for 21 days. Our results showed that 0.4 mg/kg Se supplement in broiler’s diets could improve the AFB 1 -induced histological lesions in the Cecal Tonsils including the depletion of lymphocytes in the lymphatic nodules as well as the shedding of microvilli in the absorptive cells. Moreover, Se could restore the decreased number of IgA+ cells and expression levels of pIgR, IgA, IgG, and IgM mRNA induced by AFB 1 to be close to those in the control group. These results demonstrated that 0.4 mg/kg supplemented dietary Se in the form of sodium selenite could protect the Cecal Tonsils from the histological lesions and suppression of the mucosal humoral response provoked by 0.6 mg/kg AFB 1 . Our study may provide new experimental evidences for better understanding of AFB 1 -induced damage of mucosal immunity and protective effect of Se against this toxin.
-
Short communication: Inhibitory effects of dietary aflatoxin B1 on cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens
Spanish Journal of Agricultural Research, 2016Co-Authors: Chunyu Liu, Xi Peng, Jing Fang, Min Jiang, Hengmin CuiAbstract:Aflatoxin B 1 (AFB 1 ) is the most toxic form among the mycotoxins. Cytokines are important mediators of the immune system. T-cell subsets play a crucial role in cell-mediated immunity. The aim of present study was to evaluate the effects of dietary AFB 1 on the cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens throughout a 21-day experimental period. One hundred and fifty six one-day-old broiler chickens were randomly divided into control group (0 mg AFB 1 /kg feed) and AFB 1 group (0.6 mg pure AFB 1 /kg feed). At 7, 14 and 21 days of age, the levels of seven cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α) mRNA expression as well as the proportions of T-cell subsets (CD3+, CD3+CD4+, CD3+CD8+) by qRT-PCR and flow cytometry methods were assessed in the Cecal Tonsils. The levels of the seven cytokines mRNA expression and the percentages of T-cell subsets significantly decreased at 14 and 21 days of age in the AFB 1 group compared with the control group. However, the CD4+/CD8+ ratio was not significantly changed. These results demonstrate that 0.6 mg/kg AFB 1 dietary exposure reduced the levels of cytokines mRNA expression and the percentages of T-cell subsets in the Cecal Tonsils of broiler chickens, suggesting that the cell-mediated immunity of Cecal Tonsils might be impaired in broilers.
-
Inhibitory effects of dietary aflatoxin B1 on cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens
Spanish Journal of Agricultural Research, 2016Co-Authors: Chunyu Liu, Xi Peng, Jing Fang, Min Jiang, Hengmin CuiAbstract:Aflatoxin B1 (AFB1) is the most toxic form among the mycotoxins. Cytokines are important mediators of the immune system. T-cell subsets play a crucial role in cell-mediated immunity. The aim of present study was to evaluate the effects of dietary AFB1 on the cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens throughout a 21-day experimental period. One hundred and fifty six one-day-old broiler chickens were randomly divided into control group (0 mg AFB1/kg feed) and AFB1 group (0.6 mg pure AFB1/kg feed). At 7, 14 and 21 days of age, the levels of seven cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α) mRNA expression as well as the proportions of T-cell subsets (CD3+, CD3+CD4+, CD3+CD8+) by qRT-PCR and flow cytometry methods were assessed in the Cecal Tonsils. The levels of the seven cytokines mRNA expression and the percentages of T-cell subsets significantly decreased at 14 and 21 days of age in the AFB1 group compared with the control group. However, the CD4+/CD8+ ratio was not significantly changed. These results demonstrate that 0.6 mg/kg AFB1 dietary exposure reduced the levels of cytokines mRNA expression and the percentages of T-cell subsets in the Cecal Tonsils of broiler chickens, suggesting that the cell-mediated immunity of Cecal Tonsils might be impaired in broilers.
-
dietary nickel chloride induces oxidative stress apoptosis and alters bax bcl 2 and caspase 3 mrna expression in the Cecal Tonsil of broilers
Food and Chemical Toxicology, 2014Co-Authors: Hengmin Cui, Zhicai Zuo, Xi Peng, Jing Fang, Junliang Deng, Jianying HuangAbstract:Abstract The purpose of this study was to investigate the effects of dietary NiCl 2 on antioxidant function, apoptosis, and the protein expression, mRNA expression and contents of the bcl-2, bax and caspase-3 in the Cecal Tonsil of broilers. 280 one-day-old avian broilers were divided into four groups and fed on a corn–soybean basal diet as control diet or the same basal diet supplemented with 300, 600 and 900 mg/kg of NiCl 2 for 42 days. The activities of SOD, CAT and GSH-Px, and the ability to inhibit hydroxy radical, and GSH content were significantly decreased in all experimental groups. MDA content was significantly increased. The protein expression, mRNA expression and contents of bcl-2 were decreased, and bax and caspase-3 were increased in all experimental groups. The percentages of apoptotic lymphocytes were significantly increased. In conclusion, dietary NiCl 2 in excess of 300 mg/kg caused oxidative stress, and then induced decreased the protein expression, mRNA expression and the contents of bcl-2, and increased protein expression, mRNA expression and the contents of bax and caspase-3 proteins in the Cecal Tonsil. The local intestinal mucosal immunity could finally be impaired due to the oxidative stress and apoptosis in the Cecal Tonsil caused by NiCl 2 .
Xi Peng - One of the best experts on this subject based on the ideXlab platform.
-
selenium rescues aflatoxin b 1 inhibited t cell subsets and cytokine levels in Cecal Tonsil of chickens
Biological Trace Element Research, 2019Co-Authors: Fengyuan Wang, Zhicai Zuo, Xi Peng, Jing Fang, Hengmin Cui, Kejie Chen, Gang Shu, Li TangAbstract:Cecal Tonsil is the largest peripheral lymphoid organ of the gut-associated lymphoid tissue executing immune function. To evaluate the protective effect of selenium (Se) on the Cecal Tonsil of chicken exposed to aflatoxin B1 (AFB1), 144 1-day-old healthy Cobb chickens were randomly divided into four groups, and fed with basal diet (control group), 0.6 mg/kg AFB1 (AFB1 group), 0.4 mg/kg Se supplement (+Se group), and 0.6 mg/kg AFB1 + 0.4 mg/kg Se supplement (AFB1 + Se group) for 21 days, respectively. The results showed that AFB1 significantly decreased the percentages of CD3+, CD3+CD4+, CD3+CD8+ T cells, and the CD4+/CD8+ ratio, and suppressed the expressions of IL-2, IL-4, TNF-α, and IFN-γ mRNA in the Cecal Tonsil. However, Selenium (Se) supplied in the diets restored the percentages of T cell subsets, the CD4+/CD8+ ratio, and mRNA expressions of cytokines in the AFB1 group to be close to those in the control group, and did not exhibit obvious toxicity to the Cecal Tonsil. These results indicated that Se exerted protective effect against AFB1 on the functions of Cecal Tonsil, and also partially uncovered a new role of Se that could protect Cecal Tonsil of chickens from immunotoxicity of AFB1.
-
sodium selenite prevents suppression of mucosal humoral response by afb 1 in broiler s Cecal Tonsil
Oncotarget, 2017Co-Authors: Chunyu Liu, Zhicai Zuo, Xi Peng, Jing Fang, Hengmin Cui, Panpan Zhu, Zhixiang Zheng, Yi Zhou, Ping Ouyang, Yi GengAbstract:// Chunyu Liu 1, 2, * , Zhicai Zuo 1, 2, * , Panpan Zhu 1, 2, * , Zhixiang Zheng 1, 2, * , Xi Peng 3 , Jing Fang 1, 2 , Hengmin Cui 1, 2 , Yi Zhou 4 , Ping Ouyang 2 , Yi Geng 2 , Junliang Deng 1, 2 and Yu Sun 2 1 Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China 2 College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China 3 College of Life Sciences, China West Normal University, Nanchong, Sichuan, PR China 4 Life Science Department, Sichuan Agricultural University, Yaan, Sichuan, PR China * These authors contributed equally to this work Correspondence to: Jing Fang, email: fangjing4109@163.com Xi Peng, email: pengxi197313@163.com Keywords: aflatoxin B 1 , selenium, IgA+ cell, immunoglobulin, Cecal Tonsil Received: February 06, 2017 Accepted: April 02, 2017 Published: April 13, 2017 ABSTRACT Aflatoxin B 1 (AFB 1 ), the most common mycotoxin in human food and animal feed, produces hepatotoxic, genotoxic and immunosuppressive effects in multiple species. Selenium (Se) has emerged as an important element in the dietary prevention of various toxic agents. The present study was designed to scrutinize the protective effects of sodium selenite on the histological lesions and suppression of mucosal humoral response in the Cecal Tonsil generated by AFB 1 . A total of 156 one-day-old broilers were divided into four groups and fed on basal diet (control group), 0.6 mg/kg AFB 1 (AFB 1 group), 0.4 mg/kg Se supplement (+Se group), and 0.6 mg/kg AFB 1 + 0.4 mg/kg Se supplement (AFB 1 +Se group) respectively for 21 days. Our results showed that 0.4 mg/kg Se supplement in broiler’s diets could improve the AFB 1 -induced histological lesions in the Cecal Tonsils including the depletion of lymphocytes in the lymphatic nodules as well as the shedding of microvilli in the absorptive cells. Moreover, Se could restore the decreased number of IgA+ cells and expression levels of pIgR, IgA, IgG, and IgM mRNA induced by AFB 1 to be close to those in the control group. These results demonstrated that 0.4 mg/kg supplemented dietary Se in the form of sodium selenite could protect the Cecal Tonsils from the histological lesions and suppression of the mucosal humoral response provoked by 0.6 mg/kg AFB 1 . Our study may provide new experimental evidences for better understanding of AFB 1 -induced damage of mucosal immunity and protective effect of Se against this toxin.
-
Short communication: Inhibitory effects of dietary aflatoxin B1 on cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens
Spanish Journal of Agricultural Research, 2016Co-Authors: Chunyu Liu, Xi Peng, Jing Fang, Min Jiang, Hengmin CuiAbstract:Aflatoxin B 1 (AFB 1 ) is the most toxic form among the mycotoxins. Cytokines are important mediators of the immune system. T-cell subsets play a crucial role in cell-mediated immunity. The aim of present study was to evaluate the effects of dietary AFB 1 on the cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens throughout a 21-day experimental period. One hundred and fifty six one-day-old broiler chickens were randomly divided into control group (0 mg AFB 1 /kg feed) and AFB 1 group (0.6 mg pure AFB 1 /kg feed). At 7, 14 and 21 days of age, the levels of seven cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α) mRNA expression as well as the proportions of T-cell subsets (CD3+, CD3+CD4+, CD3+CD8+) by qRT-PCR and flow cytometry methods were assessed in the Cecal Tonsils. The levels of the seven cytokines mRNA expression and the percentages of T-cell subsets significantly decreased at 14 and 21 days of age in the AFB 1 group compared with the control group. However, the CD4+/CD8+ ratio was not significantly changed. These results demonstrate that 0.6 mg/kg AFB 1 dietary exposure reduced the levels of cytokines mRNA expression and the percentages of T-cell subsets in the Cecal Tonsils of broiler chickens, suggesting that the cell-mediated immunity of Cecal Tonsils might be impaired in broilers.
-
Inhibitory effects of dietary aflatoxin B1 on cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens
Spanish Journal of Agricultural Research, 2016Co-Authors: Chunyu Liu, Xi Peng, Jing Fang, Min Jiang, Hengmin CuiAbstract:Aflatoxin B1 (AFB1) is the most toxic form among the mycotoxins. Cytokines are important mediators of the immune system. T-cell subsets play a crucial role in cell-mediated immunity. The aim of present study was to evaluate the effects of dietary AFB1 on the cytokines expression and T-cell subsets in the Cecal Tonsil of broiler chickens throughout a 21-day experimental period. One hundred and fifty six one-day-old broiler chickens were randomly divided into control group (0 mg AFB1/kg feed) and AFB1 group (0.6 mg pure AFB1/kg feed). At 7, 14 and 21 days of age, the levels of seven cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ and TNF-α) mRNA expression as well as the proportions of T-cell subsets (CD3+, CD3+CD4+, CD3+CD8+) by qRT-PCR and flow cytometry methods were assessed in the Cecal Tonsils. The levels of the seven cytokines mRNA expression and the percentages of T-cell subsets significantly decreased at 14 and 21 days of age in the AFB1 group compared with the control group. However, the CD4+/CD8+ ratio was not significantly changed. These results demonstrate that 0.6 mg/kg AFB1 dietary exposure reduced the levels of cytokines mRNA expression and the percentages of T-cell subsets in the Cecal Tonsils of broiler chickens, suggesting that the cell-mediated immunity of Cecal Tonsils might be impaired in broilers.
-
dietary nickel chloride induces oxidative stress apoptosis and alters bax bcl 2 and caspase 3 mrna expression in the Cecal Tonsil of broilers
Food and Chemical Toxicology, 2014Co-Authors: Hengmin Cui, Zhicai Zuo, Xi Peng, Jing Fang, Junliang Deng, Jianying HuangAbstract:Abstract The purpose of this study was to investigate the effects of dietary NiCl 2 on antioxidant function, apoptosis, and the protein expression, mRNA expression and contents of the bcl-2, bax and caspase-3 in the Cecal Tonsil of broilers. 280 one-day-old avian broilers were divided into four groups and fed on a corn–soybean basal diet as control diet or the same basal diet supplemented with 300, 600 and 900 mg/kg of NiCl 2 for 42 days. The activities of SOD, CAT and GSH-Px, and the ability to inhibit hydroxy radical, and GSH content were significantly decreased in all experimental groups. MDA content was significantly increased. The protein expression, mRNA expression and contents of bcl-2 were decreased, and bax and caspase-3 were increased in all experimental groups. The percentages of apoptotic lymphocytes were significantly increased. In conclusion, dietary NiCl 2 in excess of 300 mg/kg caused oxidative stress, and then induced decreased the protein expression, mRNA expression and the contents of bcl-2, and increased protein expression, mRNA expression and the contents of bax and caspase-3 proteins in the Cecal Tonsil. The local intestinal mucosal immunity could finally be impaired due to the oxidative stress and apoptosis in the Cecal Tonsil caused by NiCl 2 .
Zhicai Zuo - One of the best experts on this subject based on the ideXlab platform.
-
selenium rescues aflatoxin b 1 inhibited t cell subsets and cytokine levels in Cecal Tonsil of chickens
Biological Trace Element Research, 2019Co-Authors: Fengyuan Wang, Zhicai Zuo, Xi Peng, Jing Fang, Hengmin Cui, Kejie Chen, Gang Shu, Li TangAbstract:Cecal Tonsil is the largest peripheral lymphoid organ of the gut-associated lymphoid tissue executing immune function. To evaluate the protective effect of selenium (Se) on the Cecal Tonsil of chicken exposed to aflatoxin B1 (AFB1), 144 1-day-old healthy Cobb chickens were randomly divided into four groups, and fed with basal diet (control group), 0.6 mg/kg AFB1 (AFB1 group), 0.4 mg/kg Se supplement (+Se group), and 0.6 mg/kg AFB1 + 0.4 mg/kg Se supplement (AFB1 + Se group) for 21 days, respectively. The results showed that AFB1 significantly decreased the percentages of CD3+, CD3+CD4+, CD3+CD8+ T cells, and the CD4+/CD8+ ratio, and suppressed the expressions of IL-2, IL-4, TNF-α, and IFN-γ mRNA in the Cecal Tonsil. However, Selenium (Se) supplied in the diets restored the percentages of T cell subsets, the CD4+/CD8+ ratio, and mRNA expressions of cytokines in the AFB1 group to be close to those in the control group, and did not exhibit obvious toxicity to the Cecal Tonsil. These results indicated that Se exerted protective effect against AFB1 on the functions of Cecal Tonsil, and also partially uncovered a new role of Se that could protect Cecal Tonsil of chickens from immunotoxicity of AFB1.
-
sodium selenite prevents suppression of mucosal humoral response by afb 1 in broiler s Cecal Tonsil
Oncotarget, 2017Co-Authors: Chunyu Liu, Zhicai Zuo, Xi Peng, Jing Fang, Hengmin Cui, Panpan Zhu, Zhixiang Zheng, Yi Zhou, Ping Ouyang, Yi GengAbstract:// Chunyu Liu 1, 2, * , Zhicai Zuo 1, 2, * , Panpan Zhu 1, 2, * , Zhixiang Zheng 1, 2, * , Xi Peng 3 , Jing Fang 1, 2 , Hengmin Cui 1, 2 , Yi Zhou 4 , Ping Ouyang 2 , Yi Geng 2 , Junliang Deng 1, 2 and Yu Sun 2 1 Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China 2 College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China 3 College of Life Sciences, China West Normal University, Nanchong, Sichuan, PR China 4 Life Science Department, Sichuan Agricultural University, Yaan, Sichuan, PR China * These authors contributed equally to this work Correspondence to: Jing Fang, email: fangjing4109@163.com Xi Peng, email: pengxi197313@163.com Keywords: aflatoxin B 1 , selenium, IgA+ cell, immunoglobulin, Cecal Tonsil Received: February 06, 2017 Accepted: April 02, 2017 Published: April 13, 2017 ABSTRACT Aflatoxin B 1 (AFB 1 ), the most common mycotoxin in human food and animal feed, produces hepatotoxic, genotoxic and immunosuppressive effects in multiple species. Selenium (Se) has emerged as an important element in the dietary prevention of various toxic agents. The present study was designed to scrutinize the protective effects of sodium selenite on the histological lesions and suppression of mucosal humoral response in the Cecal Tonsil generated by AFB 1 . A total of 156 one-day-old broilers were divided into four groups and fed on basal diet (control group), 0.6 mg/kg AFB 1 (AFB 1 group), 0.4 mg/kg Se supplement (+Se group), and 0.6 mg/kg AFB 1 + 0.4 mg/kg Se supplement (AFB 1 +Se group) respectively for 21 days. Our results showed that 0.4 mg/kg Se supplement in broiler’s diets could improve the AFB 1 -induced histological lesions in the Cecal Tonsils including the depletion of lymphocytes in the lymphatic nodules as well as the shedding of microvilli in the absorptive cells. Moreover, Se could restore the decreased number of IgA+ cells and expression levels of pIgR, IgA, IgG, and IgM mRNA induced by AFB 1 to be close to those in the control group. These results demonstrated that 0.4 mg/kg supplemented dietary Se in the form of sodium selenite could protect the Cecal Tonsils from the histological lesions and suppression of the mucosal humoral response provoked by 0.6 mg/kg AFB 1 . Our study may provide new experimental evidences for better understanding of AFB 1 -induced damage of mucosal immunity and protective effect of Se against this toxin.
-
dietary nickel chloride induces oxidative stress apoptosis and alters bax bcl 2 and caspase 3 mrna expression in the Cecal Tonsil of broilers
Food and Chemical Toxicology, 2014Co-Authors: Hengmin Cui, Zhicai Zuo, Xi Peng, Jing Fang, Junliang Deng, Jianying HuangAbstract:Abstract The purpose of this study was to investigate the effects of dietary NiCl 2 on antioxidant function, apoptosis, and the protein expression, mRNA expression and contents of the bcl-2, bax and caspase-3 in the Cecal Tonsil of broilers. 280 one-day-old avian broilers were divided into four groups and fed on a corn–soybean basal diet as control diet or the same basal diet supplemented with 300, 600 and 900 mg/kg of NiCl 2 for 42 days. The activities of SOD, CAT and GSH-Px, and the ability to inhibit hydroxy radical, and GSH content were significantly decreased in all experimental groups. MDA content was significantly increased. The protein expression, mRNA expression and contents of bcl-2 were decreased, and bax and caspase-3 were increased in all experimental groups. The percentages of apoptotic lymphocytes were significantly increased. In conclusion, dietary NiCl 2 in excess of 300 mg/kg caused oxidative stress, and then induced decreased the protein expression, mRNA expression and the contents of bcl-2, and increased protein expression, mRNA expression and the contents of bax and caspase-3 proteins in the Cecal Tonsil. The local intestinal mucosal immunity could finally be impaired due to the oxidative stress and apoptosis in the Cecal Tonsil caused by NiCl 2 .
-
www.mdpi.com/journal/ijerph Article Analysis of the Toll-Like Receptor 2-2 (TLR2-2) and TLR4 mRNA Expression in the Intestinal Mucosal Immunity of Broilers Fed on Diets Supplemented with Nickel Chloride
2014Co-Authors: Hengmin Cui, Zhicai Zuo, Xi Peng, Jing Fang, Junliang Deng, Jianying HuangAbstract:Abstract: Toll-like receptor (TLRs) are important innate immune receptors, and TLR2 and TLR4 play an important role in intestinal mucosal innate immunity. It has been found that nickel (Ni) can affect the immune system in broilers. The purpose of this study was to analyze changes in TLR2-2 and TLR4 mRNA expression levels in the intestinal mucosal immunity system of broilers induced by dietary nickel chloride (NiCl2) using quantitative real-time polymerase chain reaction (qRT-PCR) assays. Two hundred and forty one-day-old avian broilers were divided into four groups and fed on a corn-soybean basal diet as control diet or the same basal diet supplemented with 300, 600 and 900 mg/kg of NiCl2 for 42 days. Results showed that the TLR2-2 and TLR4 mRNA expression levels in the intestinal mucosa and the Cecal Tonsil were lower (p < 0.05 or p < 0.01) in the 300, 600 and 900 mg/kg groups than those in the control group. It was concluded that dietary NiCl2 in excess of 300 mg/kg could reduce TLR2-2 and TLR4 mRNA expression levels in the intestinal mucosa and Cecal Tonsil in broilers, implying that the innate immunity in intestinal mucosal immune system could be impaired by pathways involving injure
-
Analysis of the Toll-Like Receptor 2-2 (TLR2-2) and TLR4 mRNA Expression in the Intestinal Mucosal Immunity of Broilers Fed on Diets Supplemented with Nickel Chloride
MDPI AG, 2014Co-Authors: Hengmin Cui, Zhicai Zuo, Xi Peng, Jing Fang, Junliang Deng, Jianying HuangAbstract:Toll-like receptor (TLRs) are important innate immune receptors, and TLR2 and TLR4 play an important role in intestinal mucosal innate immunity. It has been found that nickel (Ni) can affect the immune system in broilers. The purpose of this study was to analyze changes in TLR2-2 and TLR4 mRNA expression levels in the intestinal mucosal immunity system of broilers induced by dietary nickel chloride (NiCl2) using quantitative real-time polymerase chain reaction (qRT-PCR) assays. Two hundred and forty one-day-old avian broilers were divided into four groups and fed on a corn-soybean basal diet as control diet or the same basal diet supplemented with 300, 600 and 900 mg/kg of NiCl2 for 42 days. Results showed that the TLR2-2 and TLR4 mRNA expression levels in the intestinal mucosa and the Cecal Tonsil were lower (p < 0.05 or p < 0.01) in the 300, 600 and 900 mg/kg groups than those in the control group. It was concluded that dietary NiCl2 in excess of 300 mg/kg could reduce TLR2-2 and TLR4 mRNA expression levels in the intestinal mucosa and Cecal Tonsil in broilers, implying that the innate immunity in intestinal mucosal immune system could be impaired by pathways involving injured surface epithelium cells or/and the inhibition of the TLR signal transduction
Shayan Sharif - One of the best experts on this subject based on the ideXlab platform.
-
Probiotic Lactobacilli Limit Avian Influenza Virus Subtype H9N2 Replication in Chicken Cecal Tonsil Mononuclear Cells
Vaccines, 2020Co-Authors: Nadiyah Alqazlan, Khaled Taha-abdelaziz, Éva Nagy, Mohammadali Alizadeh, Nitish Boodhoo, Byram W. Bridle, Shayan SharifAbstract:Low pathogenic avian influenza virus (LPAIV) H9N2 poses significant threat to animal and human health. The growing interest in beneficial effects of probiotic bacteria on host immune system has led to research efforts studying their interaction with cells of host immune system. However, the role of lactobacilli in inducing antiviral responses in lymphoid tissue cells requires further investigation. The objective of the present study was to examine the antiviral and immunostimulatory effects of lactobacilli bacteria on chicken Cecal Tonsils (CT) cells against H9N2 LPAIV. CT mononuclear cells were stimulated with probiotic Lactobacillus spp mixture either alone or in combination with a Toll-like receptor (TLR)21 ligand, CpG oligodeoxynucleotides (CpG). Pre-treatment of CT cells with probiotic lactobacilli, alone or in combination with CpG, significantly reduced H9N2 LPAIV replication. Furthermore, lactobacilli alone elicited cytokine expression, including IL-2, IFN-γ, IL-1β, IL-6, and IL-12, and IL-10, while when combined with CpG, a significantly higher expression of (interferon-stimulated gene (viperin)), IL-12, IL-6, CXCLi2, and IL-1β was observed. However, none of these treatments induced significant changes in nitric oxide production by CT cells. In conclusion, probiotic lactobacilli demonstrated a modulatory effect on CT cells, and this correlated with enhanced antiviral immunity and reduced H9N2 LPAIV viral replication.
-
Campylobacter-derived ligands induce cytokine and chemokine expression in chicken macrophages and Cecal Tonsil mononuclear cells.
Veterinary microbiology, 2020Co-Authors: Khaled Taha-abdelaziz, Jake Astill, Bahram Shojadoost, Silvia Borrelli, Mario A. Monteiro, Shayan SharifAbstract:Campylobacter jejuni colonizes the chicken gut at a high density without causing disease. However, consumption of poultry products contaminated with this bacterium causes gastroenteritis in humans. Therefore, it is critically important to reduce the Campylobacter burden in poultry products to prevent transmission to humans. Evidence indicates that enhancing intestinal mucosal immune responses is of paramount importance for preventing or reducing Campylobacter colonization in chickens. In view of this, the present study was undertaken to evaluate host responses to different C. jejuni-derived ligands, including lipooligosaccharide (LOS), outer membrane proteins (OMPs), and genomic DNA, with the ultimate goal of identifying a ligand with potent immunostimulatory capacity to serve as a mucosal vaccine adjuvant against enteric infections in chickens. The results revealed that C. jejuni pathogen-associated molecular patterns (PAMPs) varied in their ability to induce the expression of cytokines and chemokines in chicken macrophages and Cecal Tonsil mononuclear cells and nitric oxide production in macrophages. In addition, C. jejuni OMPs demonstrated superior activity over LOS and DNA ligands in eliciting cytokine expression associated with T helper (Th)1 and Th2 responses (interferon [IFN]-γ and interleukin [IL]-13, respectively), in addition to expression of pro-inflammatory cytokines (IL-1β), chemokine (CXCLi2), and regulatory cytokines (IL-10 and TGFβ1/4) in Cecal Tonsil cells. Importantly, in addition to their ability to induce innate responses, OMPs could also function as antigens to elicit C. jejuni-specific antibody responses and thereby confer dual protection against C. jejuni infection. Further studies are required to assess the protective efficacy of C. jejuni OMPs against C. jejuni infection in chickens.
-
Characterization of host responses induced by Toll-like receptor ligands in chicken Cecal Tonsil cells.
Veterinary immunology and immunopathology, 2016Co-Authors: Khaled Taha-abdelaziz, Bahram Shojadoost, Tamiru Negash Alkie, Douglas C. Hodgins, Shayan SharifAbstract:The innate responses of Cecal Tonsils against invading microorganisms are mediated by conserved pattern recognition receptors (PRRs) such as the Toll-like receptors (TLRs). TLRs expressed by mammalian and avian immune system cells have the capability to recognize pathogen-associated molecular patterns (PAMPs). Although, the role of TLR ligands in innate and adaptive responses in chickens has been characterized in spleen and bursa of Fabricius, considerably less is known about responses in Cecal Tonsils. The aim of the current study was to assess responses of mononuclear cells from Cecal Tonsils to treatment with the TLR2, TLR4 and TLR21 ligands, Pam3CSK4, lipopolysaccharide (LPS), and CpG oligodeoxynucleotide (ODN), respectively. All three ligands induced significant up-regulation of interferon (IFN)-γ, interleukin (IL)-1β, IL-6 and CxCLi2/IL-8, whereas no significant changes were observed in expression of IL-13 or the antimicrobial peptides, avian β-defensin (AvBD) 1, AvBD2 and cathelicidin 3 (CATHL-3). In general, CpG ODN elicited the highest cytokine responses by Cecal Tonsil mononuclear cells, inducing significantly higher expression compared to LPS and Pam3CSK4, for IFNγ, IL-1β, IL-6 and CxCLi2 at various time points. These findings suggest the potential use of TLR21 ligands as mucosal vaccine adjuvants, especially in the context of pathogens of the intestinal tract.
-
Effects of Lactobacilli on Cytokine Expression by Chicken Spleen and Cecal Tonsil Cells
Clinical and vaccine immunology : CVI, 2010Co-Authors: Jennifer T. Brisbin, Joshua Gong, Payvand Parvizi, Shayan SharifAbstract:Lactobacillus acidophilus, Lactobacillus reuteri, and Lactobacillus salivarius are all normal residents of the chicken gastrointestinal tract. Given the interest in using probiotic bacteria in chicken production and the important role of the microbiota in the development and regulation of the host immune system, the objective of the current study was to examine the differential effects of these bacteria on cytokine gene expression profiles of lymphoid tissue cells. Mononuclear cells isolated from Cecal Tonsils and spleens of chickens were cocultured with one of the three live bacteria, and gene expression was analyzed via real-time quantitative PCR. All three lactobacilli induced significantly more interleukin 1β (IL-1β) expression in spleen cells than in Cecal Tonsil cells, indicating a more inflammatory response in the spleen than in Cecal Tonsils. In Cecal Tonsil cells, substantial differences were found among strains in the capacity to induce IL-12p40, IL-10, IL-18, transforming growth factor β4 (TGF-β4), and gamma interferon (IFN-γ). In conclusion, we demonstrated that L. acidophilus is more effective at inducing T-helper-1 cytokines while L. salivarius induces a more anti-inflammatory response.