The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform

Chunsheng Wang - One of the best experts on this subject based on the ideXlab platform.

  • synthetic eptfe grafts coated with an anti cd133 antibody functionalized heparin collagen multilayer with rapid in vivo endothelialization properties
    ACS Applied Materials & Interfaces, 2013
    Co-Authors: Shuyang Lu, Peng Zhang, Feirong Gong, Shouguo Yang, Li Shen, Zheyong Huang, Chunsheng Wang
    Abstract:

    An anti-CD133 antibody multilayer functionalized by heparin/collagen on an expanded polytetrafluoroethylene (ePTFE) graft was developed to accelerate early endothelialization. The surface modification of ePTFE grafts demonstrated that the multilayer is stable in static incubation and shaking conditions and that the anti-CD133 antibodies were successfully cross-linked onto the surface. Blood compatibility tests revealed that the coimmobilized heparin/collagen films in the presence or absence of anti-CD133 antibodies prolonged the blood coagulation time and that there was less platelet activation and aggregation, whereas the hemolysis rate was comparable with the bare ePTFE grafts. Cellular proliferation was not inhibited, as the heparin/collagen synthetic vascular grafts coated with CD133 antibody showed little cytotoxicity. The endothelial Cells adhered well to the modified ePTFE grafts during a Cell Adhesion Assay. A porcine carotid artery transplantation model was used to evaluate the modified ePTFE gra...

  • Synthetic ePTFE grafts coated with an anti-CD133 antibody-functionalized heparin/collagen multilayer with rapid in vivo endothelialization properties.
    ACS applied materials & interfaces, 2013
    Co-Authors: Peng Zhang, Feirong Gong, Shouguo Yang, Li Shen, Zheyong Huang, Xiaoning Sun, Chunsheng Wang
    Abstract:

    An anti-CD133 antibody multilayer functionalized by heparin/collagen on an expanded polytetrafluoroethylene (ePTFE) graft was developed to accelerate early endothelialization. The surface modification of ePTFE grafts demonstrated that the multilayer is stable in static incubation and shaking conditions and that the anti-CD133 antibodies were successfully cross-linked onto the surface. Blood compatibility tests revealed that the coimmobilized heparin/collagen films in the presence or absence of anti-CD133 antibodies prolonged the blood coagulation time and that there was less platelet activation and aggregation, whereas the hemolysis rate was comparable with the bare ePTFE grafts. Cellular proliferation was not inhibited, as the heparin/collagen synthetic vascular grafts coated with CD133 antibody showed little cytotoxicity. The endothelial Cells adhered well to the modified ePTFE grafts during a Cell Adhesion Assay. A porcine carotid artery transplantation model was used to evaluate the modified ePTFE grafts in vivo. The results of histopathological staining and scanning electron microscopy indicated that the anti-CD133 antibody was able to accelerate the attachment of vascular endothelial Cells onto the ePTFE grafts, resulting in early rapid endothelialization. The success of the anti-CD133 antibody-functionalized heparin/collagen multilayer will provide an effective selection system for the surface modification of synthetic vascular grafts and improve their use in clinical applications.

  • synthetic eptfe grafts coated with an anti cd133 antibody functionalized heparin collagen multilayer with rapid in vivo endothelialization properties
    ACS Applied Materials & Interfaces, 2013
    Co-Authors: Peng Zhang, Feirong Gong, Shouguo Yang, Li Shen, Zheyong Huang, Xiaoning Sun, Chunsheng Wang
    Abstract:

    An anti-CD133 antibody multilayer functionalized by heparin/collagen on an expanded polytetrafluoroethylene (ePTFE) graft was developed to accelerate early endothelialization. The surface modification of ePTFE grafts demonstrated that the multilayer is stable in static incubation and shaking conditions and that the anti-CD133 antibodies were successfully cross-linked onto the surface. Blood compatibility tests revealed that the coimmobilized heparin/collagen films in the presence or absence of anti-CD133 antibodies prolonged the blood coagulation time and that there was less platelet activation and aggregation, whereas the hemolysis rate was comparable with the bare ePTFE grafts. Cellular proliferation was not inhibited, as the heparin/collagen synthetic vascular grafts coated with CD133 antibody showed little cytotoxicity. The endothelial Cells adhered well to the modified ePTFE grafts during a Cell Adhesion Assay. A porcine carotid artery transplantation model was used to evaluate the modified ePTFE grafts in vivo. The results of histopathological staining and scanning electron microscopy indicated that the anti-CD133 antibody was able to accelerate the attachment of vascular endothelial Cells onto the ePTFE grafts, resulting in early rapid endothelialization. The success of the anti-CD133 antibody-functionalized heparin/collagen multilayer will provide an effective selection system for the surface modification of synthetic vascular grafts and improve their use in clinical applications.

Clarke F. Millette - One of the best experts on this subject based on the ideXlab platform.

  • Sertoli Cell Plasma Membrane Polypeptides Involved in Spermatogenic Cell‐Sertoli Cell Adhesion
    Journal of andrology, 1992
    Co-Authors: Sean C. Newton, Clarke F. Millette
    Abstract:

    : This study concerns Sertoli Cell-spermatogenic Cell adhesive interactions in the seminiferous tubule. Sertoli Cell surface polypeptides involved in germ Cell-Sertoli Cell Adhesion were identified by serological inhibition of an in vitro Sertoli-germ Cell Adhesion Assay. This Assay was modified from a previously reported Adhesion Assay, and employs a scanning laser cytometer for quantification of adherent Cells. Reactivity of the polyclonal antiserum raised against rat Sertoli Cells was also assessed via immunofluorescent microscopy. The addition of antiserum to the Adhesion Assay resulted in a 42% to 66% inhibition of Cell-Cell Adhesion. Moreover, preincubation of antiserum with Sertoli Cell monolayers resulted in a significant reduction of spermatogenic Cell binding. Conversely, preincubation of antiserum with germ Cells resulted in no reduction. Western blot analysis of the antiserum against purified Sertoli Cell membranes indicated reactivity with four polypeptides. The data suggest that one or more of these polypeptides are directly involved in the Adhesion of germ Cells to Sertoli Cell monolayers in vitro.

  • Quantification of an in vitro Cell-Cell Adhesion Assay using interactive laser scanning cytometry.
    Cytometry, 1992
    Co-Authors: Sean C. Newton, Clarke F. Millette
    Abstract:

    We are interested in identifying Cell-Cell Adhesion molecules on the surface of Sertoli Cells that mediate Sertoli Cell-spermatogenic Cell Adhesion. Numerous Cell-Cell Adhesion Assays employ microscopic observation, photomicroscopy or radioactive isotopes for quantification. Previously, we developed an in vitro Assay for testicular Cell interactions. This Assay was, however, time consuming using photography for analysis. We have now modified this system using laser cytometry to quantify adherent Cells. Rat testicular epithelial Cells are cultured for approximately 6 days before labelling with fluorescein diactetate (FDA) to assess confluency by image scanning so that spermatogenic Cell binding can be normalized to available epithelial Cell surface area. Rat spermatogenic Cells are labeled with FDA before addition to epithelial Cell monolayers. In some studies, purified spermatogenic Cell populations were isolated to determine average Cell size. We found that spermatocyte area varied between 225-500 microns2, spermatids were 100-225 microns2 and residual bodies were less than 100 microns2. Using these parameters, scanning cytometry allows the differential analysis of Adhesion by individual germ Cell sub-classes from mixed Cell suspensions, saving time, animals, and major expense. The scanning laser assisted Assay is faster, more reproducible and less subjective than earlier Cell-Cell Adhesion Assays using light microscopy or isotopes. This experimental approach should facilitate any Cell-Cell Adhesion Assay in which one Cell type is adherent to a substrate.

Peng Zhang - One of the best experts on this subject based on the ideXlab platform.

  • synthetic eptfe grafts coated with an anti cd133 antibody functionalized heparin collagen multilayer with rapid in vivo endothelialization properties
    ACS Applied Materials & Interfaces, 2013
    Co-Authors: Shuyang Lu, Peng Zhang, Feirong Gong, Shouguo Yang, Li Shen, Zheyong Huang, Chunsheng Wang
    Abstract:

    An anti-CD133 antibody multilayer functionalized by heparin/collagen on an expanded polytetrafluoroethylene (ePTFE) graft was developed to accelerate early endothelialization. The surface modification of ePTFE grafts demonstrated that the multilayer is stable in static incubation and shaking conditions and that the anti-CD133 antibodies were successfully cross-linked onto the surface. Blood compatibility tests revealed that the coimmobilized heparin/collagen films in the presence or absence of anti-CD133 antibodies prolonged the blood coagulation time and that there was less platelet activation and aggregation, whereas the hemolysis rate was comparable with the bare ePTFE grafts. Cellular proliferation was not inhibited, as the heparin/collagen synthetic vascular grafts coated with CD133 antibody showed little cytotoxicity. The endothelial Cells adhered well to the modified ePTFE grafts during a Cell Adhesion Assay. A porcine carotid artery transplantation model was used to evaluate the modified ePTFE gra...

  • Synthetic ePTFE grafts coated with an anti-CD133 antibody-functionalized heparin/collagen multilayer with rapid in vivo endothelialization properties.
    ACS applied materials & interfaces, 2013
    Co-Authors: Peng Zhang, Feirong Gong, Shouguo Yang, Li Shen, Zheyong Huang, Xiaoning Sun, Chunsheng Wang
    Abstract:

    An anti-CD133 antibody multilayer functionalized by heparin/collagen on an expanded polytetrafluoroethylene (ePTFE) graft was developed to accelerate early endothelialization. The surface modification of ePTFE grafts demonstrated that the multilayer is stable in static incubation and shaking conditions and that the anti-CD133 antibodies were successfully cross-linked onto the surface. Blood compatibility tests revealed that the coimmobilized heparin/collagen films in the presence or absence of anti-CD133 antibodies prolonged the blood coagulation time and that there was less platelet activation and aggregation, whereas the hemolysis rate was comparable with the bare ePTFE grafts. Cellular proliferation was not inhibited, as the heparin/collagen synthetic vascular grafts coated with CD133 antibody showed little cytotoxicity. The endothelial Cells adhered well to the modified ePTFE grafts during a Cell Adhesion Assay. A porcine carotid artery transplantation model was used to evaluate the modified ePTFE grafts in vivo. The results of histopathological staining and scanning electron microscopy indicated that the anti-CD133 antibody was able to accelerate the attachment of vascular endothelial Cells onto the ePTFE grafts, resulting in early rapid endothelialization. The success of the anti-CD133 antibody-functionalized heparin/collagen multilayer will provide an effective selection system for the surface modification of synthetic vascular grafts and improve their use in clinical applications.

  • synthetic eptfe grafts coated with an anti cd133 antibody functionalized heparin collagen multilayer with rapid in vivo endothelialization properties
    ACS Applied Materials & Interfaces, 2013
    Co-Authors: Peng Zhang, Feirong Gong, Shouguo Yang, Li Shen, Zheyong Huang, Xiaoning Sun, Chunsheng Wang
    Abstract:

    An anti-CD133 antibody multilayer functionalized by heparin/collagen on an expanded polytetrafluoroethylene (ePTFE) graft was developed to accelerate early endothelialization. The surface modification of ePTFE grafts demonstrated that the multilayer is stable in static incubation and shaking conditions and that the anti-CD133 antibodies were successfully cross-linked onto the surface. Blood compatibility tests revealed that the coimmobilized heparin/collagen films in the presence or absence of anti-CD133 antibodies prolonged the blood coagulation time and that there was less platelet activation and aggregation, whereas the hemolysis rate was comparable with the bare ePTFE grafts. Cellular proliferation was not inhibited, as the heparin/collagen synthetic vascular grafts coated with CD133 antibody showed little cytotoxicity. The endothelial Cells adhered well to the modified ePTFE grafts during a Cell Adhesion Assay. A porcine carotid artery transplantation model was used to evaluate the modified ePTFE grafts in vivo. The results of histopathological staining and scanning electron microscopy indicated that the anti-CD133 antibody was able to accelerate the attachment of vascular endothelial Cells onto the ePTFE grafts, resulting in early rapid endothelialization. The success of the anti-CD133 antibody-functionalized heparin/collagen multilayer will provide an effective selection system for the surface modification of synthetic vascular grafts and improve their use in clinical applications.

Andrés J. García - One of the best experts on this subject based on the ideXlab platform.

  • A centrifugation Cell Adhesion Assay for high-throughput screening of biomaterial surfaces.
    Journal of biomedical materials research. Part A, 2003
    Co-Authors: Catherine D. Reyes, Andrés J. García
    Abstract:

    A quantitative analysis of Cell Adhesion is essential in understanding physiological phenomena and designing biomaterials, implant surfaces, and tissue-engineering scaffolds. The most common Cell Adhesion Assays used to evaluate biomaterial surfaces lack sensitivity and reproducibility and/or require specialized equipment and skill-intensive operation. We describe a modified centrifugation Cell Adhesion Assay that uses simple and convenient techniques with standard laboratory equipment and provides reliable, quantitative measurements of Cell Adhesion. This centrifugation Assay applies controlled and uniform detachment forces to a large population of adherent Cells, providing robust statistics for quantifying Cell Adhesion. The applicability of this system to the design and characterization of biomaterial surfaces is shown by evaluating Cell Adhesion on substrates using different coating proteins, Cell types, seeding times, and relative centrifugal forces (RCF). Results verify that this centrifugation Cell Adhesion Assay represents a simple, convenient, and standard method for high-throughput characterization of a variety of biomaterial surfaces and conditions.

  • a centrifugation Cell Adhesion Assay for high throughput screening of biomaterial surfaces
    Journal of Biomedical Materials Research Part A, 2003
    Co-Authors: Catherine D. Reyes, Andrés J. García
    Abstract:

    A quantitative analysis of Cell Adhesion is essential in understanding physiological phenomena and designing biomaterials, implant surfaces, and tissue-engineering scaffolds. The most common Cell Adhesion Assays used to evaluate biomaterial surfaces lack sensitivity and reproducibility and/or require specialized equipment and skill-intensive operation. We describe a modified centrifugation Cell Adhesion Assay that uses simple and convenient techniques with standard laboratory equipment and provides reliable, quantitative measurements of Cell Adhesion. This centrifugation Assay applies controlled and uniform detachment forces to a large population of adherent Cells, providing robust statistics for quantifying Cell Adhesion. The applicability of this system to the design and characterization of biomaterial surfaces is shown by evaluating Cell Adhesion on substrates using different coating proteins, Cell types, seeding times, and relative centrifugal forces (RCF). Results verify that this centrifugation Cell Adhesion Assay represents a simple, convenient, and standard method for high-throughput characterization of a variety of biomaterial surfaces and conditions. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 328–333, 2003

Sean C. Newton - One of the best experts on this subject based on the ideXlab platform.

  • Sertoli Cell Plasma Membrane Polypeptides Involved in Spermatogenic Cell‐Sertoli Cell Adhesion
    Journal of andrology, 1992
    Co-Authors: Sean C. Newton, Clarke F. Millette
    Abstract:

    : This study concerns Sertoli Cell-spermatogenic Cell adhesive interactions in the seminiferous tubule. Sertoli Cell surface polypeptides involved in germ Cell-Sertoli Cell Adhesion were identified by serological inhibition of an in vitro Sertoli-germ Cell Adhesion Assay. This Assay was modified from a previously reported Adhesion Assay, and employs a scanning laser cytometer for quantification of adherent Cells. Reactivity of the polyclonal antiserum raised against rat Sertoli Cells was also assessed via immunofluorescent microscopy. The addition of antiserum to the Adhesion Assay resulted in a 42% to 66% inhibition of Cell-Cell Adhesion. Moreover, preincubation of antiserum with Sertoli Cell monolayers resulted in a significant reduction of spermatogenic Cell binding. Conversely, preincubation of antiserum with germ Cells resulted in no reduction. Western blot analysis of the antiserum against purified Sertoli Cell membranes indicated reactivity with four polypeptides. The data suggest that one or more of these polypeptides are directly involved in the Adhesion of germ Cells to Sertoli Cell monolayers in vitro.

  • Quantification of an in vitro Cell-Cell Adhesion Assay using interactive laser scanning cytometry.
    Cytometry, 1992
    Co-Authors: Sean C. Newton, Clarke F. Millette
    Abstract:

    We are interested in identifying Cell-Cell Adhesion molecules on the surface of Sertoli Cells that mediate Sertoli Cell-spermatogenic Cell Adhesion. Numerous Cell-Cell Adhesion Assays employ microscopic observation, photomicroscopy or radioactive isotopes for quantification. Previously, we developed an in vitro Assay for testicular Cell interactions. This Assay was, however, time consuming using photography for analysis. We have now modified this system using laser cytometry to quantify adherent Cells. Rat testicular epithelial Cells are cultured for approximately 6 days before labelling with fluorescein diactetate (FDA) to assess confluency by image scanning so that spermatogenic Cell binding can be normalized to available epithelial Cell surface area. Rat spermatogenic Cells are labeled with FDA before addition to epithelial Cell monolayers. In some studies, purified spermatogenic Cell populations were isolated to determine average Cell size. We found that spermatocyte area varied between 225-500 microns2, spermatids were 100-225 microns2 and residual bodies were less than 100 microns2. Using these parameters, scanning cytometry allows the differential analysis of Adhesion by individual germ Cell sub-classes from mixed Cell suspensions, saving time, animals, and major expense. The scanning laser assisted Assay is faster, more reproducible and less subjective than earlier Cell-Cell Adhesion Assays using light microscopy or isotopes. This experimental approach should facilitate any Cell-Cell Adhesion Assay in which one Cell type is adherent to a substrate.