The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform

Kenton W Gregory - One of the best experts on this subject based on the ideXlab platform.

  • mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials
    Acta Biomaterialia, 2012
    Co-Authors: Kathryn A Mckenna, Monica T Hinds, Rebecca C Sarao, Ping Cheng Wu, Cheryl L Maslen, Robert W Glanville, Darcie Babcock, Kenton W Gregory
    Abstract:

    The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular Cell Compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1 cm diameter prTE samples were constructed for uniaxial tensile testing and 4 mm small-diameter prTE tubular scaffolds were produced for burst pressure and Cell Compatibility evaluations from 15 wt% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36±0.05 MPa and elastic moduli of 0.15±0.04 MPa and 0.91±0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485 ± 25 mmHg were obtained from 4 mm ID scaffolds with 453 ± 74 μm average wall thickness. prTE supported endothelial Cell growth with typical endothelial Cell cobblestone morphology after 48 hours in culture. Cross-linked electrospun recombinant human tropoelastin has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix, and vascular Cell Compatibility.

  • mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials
    Acta Biomaterialia, 2012
    Co-Authors: Kathryn A Mckenna, Monica T Hinds, Rebecca C Sarao, Cheryl L Maslen, Robert W Glanville, Darcie Babcock, Kenton W Gregory
    Abstract:

    The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular Cell Compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1cm diameter prTE samples were constructed for uniaxial tensile testing and 4mm small-diameter prTE tubular scaffolds were produced for burst pressure and Cell Compatibility evaluations from 15 wt.% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36±0.05 MPa and elastic moduli of 0.15±0.04 and 0.91±0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485±25 mm Hg were obtained from 4mm internal diameter scaffolds with 453±74 μm average wall thickness. prTE supported endothelial Cell growth with typical endothelial Cell cobblestone morphology after 48 h in culture. Cross-linked electrospun rTE has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix and vascular Cell Compatibility.

Kathryn A Mckenna - One of the best experts on this subject based on the ideXlab platform.

  • mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials
    Acta Biomaterialia, 2012
    Co-Authors: Kathryn A Mckenna, Monica T Hinds, Rebecca C Sarao, Ping Cheng Wu, Cheryl L Maslen, Robert W Glanville, Darcie Babcock, Kenton W Gregory
    Abstract:

    The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular Cell Compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1 cm diameter prTE samples were constructed for uniaxial tensile testing and 4 mm small-diameter prTE tubular scaffolds were produced for burst pressure and Cell Compatibility evaluations from 15 wt% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36±0.05 MPa and elastic moduli of 0.15±0.04 MPa and 0.91±0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485 ± 25 mmHg were obtained from 4 mm ID scaffolds with 453 ± 74 μm average wall thickness. prTE supported endothelial Cell growth with typical endothelial Cell cobblestone morphology after 48 hours in culture. Cross-linked electrospun recombinant human tropoelastin has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix, and vascular Cell Compatibility.

  • mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials
    Acta Biomaterialia, 2012
    Co-Authors: Kathryn A Mckenna, Monica T Hinds, Rebecca C Sarao, Cheryl L Maslen, Robert W Glanville, Darcie Babcock, Kenton W Gregory
    Abstract:

    The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular Cell Compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1cm diameter prTE samples were constructed for uniaxial tensile testing and 4mm small-diameter prTE tubular scaffolds were produced for burst pressure and Cell Compatibility evaluations from 15 wt.% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36±0.05 MPa and elastic moduli of 0.15±0.04 and 0.91±0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485±25 mm Hg were obtained from 4mm internal diameter scaffolds with 453±74 μm average wall thickness. prTE supported endothelial Cell growth with typical endothelial Cell cobblestone morphology after 48 h in culture. Cross-linked electrospun rTE has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix and vascular Cell Compatibility.

Wankei Wan - One of the best experts on this subject based on the ideXlab platform.

  • exploring Cell Compatibility of a fibronectin functionalized physically crosslinked poly vinyl alcohol hydrogel
    Journal of Biomedical Materials Research Part B, 2012
    Co-Authors: Leonardo E Millon, Donna T Padavan, Amanda M Hamilton, Derek R Boughner, Wankei Wan
    Abstract:

    Physically crosslinked poly(vinyl alcohol) (PVA) hydrogels prepared using a low-temperature thermally cycled process have tunable mechanical properties that fall within the range of soft tissues, including cardiovascular tissue. An approach to render it hemocompatible is by endothelization, but its hydrophilic nature is not conducive to Cell adhesion and spreading. We investigated the functionalization reaction of this class of PVA hydrogel with fibronectin (FN) for adhesion and spreading of primary porcine radial artery Cells and vascular endothelial Cells. These are Cells relevant to small-diameter vascular graft development. FN functionalization was achieved using a multistep reaction, but the activation step involving carbonyl diimidazole normally required for chemically crosslinked PVA was found to be unnecessary. The reaction resulted in an increase in the elastic modulus of the PVA hydrogel but is still well within the range of cardiovascular tissue. Confocal microscopy confirmed the adhesion and spreading of both Cell types on the PVA–FN surfaces, whereas Cells failed to adhere to the PVA control. This is a first step toward an alternative for the realization of a synthetic replacement small-diameter vascular graft. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.

  • synthesis characterization and in vitro Cell Compatibility study of a poly amic acid graft cross linked poly vinyl alcohol hydrogel
    Acta Biomaterialia, 2011
    Co-Authors: Donna T Padavan, Amanda M Hamilton, Leonardo E Millon, Derek R Boughner, Wankei Wan
    Abstract:

    Although physically cross-linked poly(vinyl alcohol) (PVA) hydrogels have tunable mechanical properties to match that of soft tissues, such as vascular tissue, their hydrophilic nature is not conducive to Cell adhesion and spreading. For applications such as small diameter vascular grafts for coronary bypass both mechanical matching and hemoCompatibility are important. Poly(amic acid) (PAA), derived from ethylene diamine tetraacetic dianhydride, is a Cell-compatible polymer. It was grafted/cross-linked onto physically cross-linked PVA to provide Cell Compatibility. Functionalization was achieved via a one-step esterification reaction using 1,3-dicyclohexylcarbodiimide as the coupling agent and 4-dimethylaminopyridine as the catalyst. The success of the grafting reaction was verified using Fourier transform infrared spectroscopy, solid-state nuclear magnetic resonance spectroscopy and X-ray photoelectron spectroscopy. The mechanical properties of the starting PVA hydrogel were largely preserved after the grafting reaction within the physiological strain range of vascular tissue. In vitro Cell culture studies using primary porcine endothelial Cells confirmed Cell Compatibility of the PAA graft PVA hydrogel, making it an attractive candidate for small diameter vascular graft development.

Cheryl L Maslen - One of the best experts on this subject based on the ideXlab platform.

  • mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials
    Acta Biomaterialia, 2012
    Co-Authors: Kathryn A Mckenna, Monica T Hinds, Rebecca C Sarao, Ping Cheng Wu, Cheryl L Maslen, Robert W Glanville, Darcie Babcock, Kenton W Gregory
    Abstract:

    The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular Cell Compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1 cm diameter prTE samples were constructed for uniaxial tensile testing and 4 mm small-diameter prTE tubular scaffolds were produced for burst pressure and Cell Compatibility evaluations from 15 wt% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36±0.05 MPa and elastic moduli of 0.15±0.04 MPa and 0.91±0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485 ± 25 mmHg were obtained from 4 mm ID scaffolds with 453 ± 74 μm average wall thickness. prTE supported endothelial Cell growth with typical endothelial Cell cobblestone morphology after 48 hours in culture. Cross-linked electrospun recombinant human tropoelastin has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix, and vascular Cell Compatibility.

  • mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials
    Acta Biomaterialia, 2012
    Co-Authors: Kathryn A Mckenna, Monica T Hinds, Rebecca C Sarao, Cheryl L Maslen, Robert W Glanville, Darcie Babcock, Kenton W Gregory
    Abstract:

    The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular Cell Compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1cm diameter prTE samples were constructed for uniaxial tensile testing and 4mm small-diameter prTE tubular scaffolds were produced for burst pressure and Cell Compatibility evaluations from 15 wt.% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36±0.05 MPa and elastic moduli of 0.15±0.04 and 0.91±0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485±25 mm Hg were obtained from 4mm internal diameter scaffolds with 453±74 μm average wall thickness. prTE supported endothelial Cell growth with typical endothelial Cell cobblestone morphology after 48 h in culture. Cross-linked electrospun rTE has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix and vascular Cell Compatibility.

Robert W Glanville - One of the best experts on this subject based on the ideXlab platform.

  • mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials
    Acta Biomaterialia, 2012
    Co-Authors: Kathryn A Mckenna, Monica T Hinds, Rebecca C Sarao, Ping Cheng Wu, Cheryl L Maslen, Robert W Glanville, Darcie Babcock, Kenton W Gregory
    Abstract:

    The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular Cell Compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1 cm diameter prTE samples were constructed for uniaxial tensile testing and 4 mm small-diameter prTE tubular scaffolds were produced for burst pressure and Cell Compatibility evaluations from 15 wt% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36±0.05 MPa and elastic moduli of 0.15±0.04 MPa and 0.91±0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485 ± 25 mmHg were obtained from 4 mm ID scaffolds with 453 ± 74 μm average wall thickness. prTE supported endothelial Cell growth with typical endothelial Cell cobblestone morphology after 48 hours in culture. Cross-linked electrospun recombinant human tropoelastin has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix, and vascular Cell Compatibility.

  • mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials
    Acta Biomaterialia, 2012
    Co-Authors: Kathryn A Mckenna, Monica T Hinds, Rebecca C Sarao, Cheryl L Maslen, Robert W Glanville, Darcie Babcock, Kenton W Gregory
    Abstract:

    The development of vascular grafts has focused on finding a biomaterial that is non-thrombogenic, minimizes intimal hyperplasia, matches the mechanical properties of native vessels and allows for regeneration of arterial tissue. In this study, the structural and mechanical properties and the vascular Cell Compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix. Disuccinimidyl suberate (DSS) was used to cross-link electrospun rTE fibers to produce a polymeric recombinant tropoelastin (prTE) matrix that is stable in aqueous environments. Tubular 1cm diameter prTE samples were constructed for uniaxial tensile testing and 4mm small-diameter prTE tubular scaffolds were produced for burst pressure and Cell Compatibility evaluations from 15 wt.% rTE solutions. Uniaxial tensile tests demonstrated an average ultimate tensile strength (UTS) of 0.36±0.05 MPa and elastic moduli of 0.15±0.04 and 0.91±0.16 MPa, which were comparable to extracted native elastin. Burst pressures of 485±25 mm Hg were obtained from 4mm internal diameter scaffolds with 453±74 μm average wall thickness. prTE supported endothelial Cell growth with typical endothelial Cell cobblestone morphology after 48 h in culture. Cross-linked electrospun rTE has promising properties for utilization as a vascular graft biomaterial with customizable dimensions, a compliant matrix and vascular Cell Compatibility.