The Experts below are selected from a list of 200265 Experts worldwide ranked by ideXlab platform
Joe Lutkenhaus - One of the best experts on this subject based on the ideXlab platform.
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eLS - Bacterial Cell Division
Emerging Targets in Antibacterial and Antifungal Chemotherapy, 1992Co-Authors: Joe LutkenhausAbstract:The process of Cell Division is necessary for the growth of all Cells. The mechanism by which eubacteria divide has so far shown little homology to the mechanisms employed by eukaryotic Cells. Although this lack of similarity may be a result of our lack of knowledge about these processes it may also be that different mechanisms are involved. If so, it would make Cell Division a selective target for antimicrobial agents.
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FtsZ and Cell Division.
Research in microbiology, 1991Co-Authors: Kang Dai, S. Subbarao, B. Beall, Joe LutkenhausAbstract:The ftsZ gene in Escherichia coli is thought to be an essential gene and to play a pivotal role in Cell Division. Gene disruption experiments confirmed that ftsZ is an essential gene. Examination of Cellular responses to FtsZ depletion indicated that FtsZ was required for Division but not for nucleoid segregation. Analysis of mutations within the ftsZ, gene, selected for resistance to the Cell Division inhibitor SulA, revealed that they also conferred resistance to MinCD. This raises the possibility that ftsZ is the target of these two Cell Division inhibitors. Analysis of the ftsZ gene from Bacillus subtilis revealed that the gene was required for both septation during vegetative growth and asymmetric septation during sporulation.
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Regulation of Cell Division in E. coli.
Trends in genetics : TIG, 1990Co-Authors: Joe LutkenhausAbstract:Recent investigation of some old Cell Division mutants of E. coli suggests that genes playing central roles in the regulation of Division have been identified. The results suggest that Cell Division is triggered when a critical level of a single protein, FtsZ, is attained. The activity of this protein is channelled to the new Division site by the activity of the min locus, which blocks access to old sites. Continued study of these genes should yield further insights into the Cell Division process.
Jo-anne R. Dillon - One of the best experts on this subject based on the ideXlab platform.
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The distinctive Cell Division interactome of Neisseria gonorrhoeae.
BMC microbiology, 2017Co-Authors: Yinan Zou, Jo-anne R. DillonAbstract:Bacterial Cell Division is an essential process driven by the formation of a Z-ring structure, as a cytoskeletal scaffold at the mid-Cell, followed by the recruitment of various proteins which form the divisome. The Cell Division interactome reflects the complement of different interactions between all divisome proteins. To date, only two Cell Division interactomes have been characterized, in Escherichia coli and in Streptococcus pneumoniae. The Cell divison proteins encoded by Neisseria gonorrhoeae include FtsZ, FtsA, ZipA, FtsK, FtsQ, FtsI, FtsW, and FtsN. The purpose of the present study was to characterize the Cell Division interactome of N. gonorrhoeae using several different methods to identify protein-protein interactions. We also characterized the specific subdomains of FtsA implicated in interactions with FtsZ, FtsQ, FtsN and FtsW. Using a combination of bacterial two-hybrid (B2H), glutathione S-transferase (GST) pull-down assays, and surface plasmon resonance (SPR), nine interactions were observed among the eight gonococcal Cell Division proteins tested. ZipA did not interact with any other Cell Division proteins. Comparisons of the N. gonorrhoeae Cell Division interactome with the published interactomes from E. coli and S. pneumoniae indicated that FtsA-FtsZ and FtsZ-FtsK interactions were common to all three species. FtsA-FtsW and FtsK-FtsN interactions were only present in N. gonorrhoeae. The 2A and 2B subdomains of FtsANg were involved in interactions with FtsQ, FtsZ, and FtsN, and the 2A subdomain was involved in interaction with FtsW. Results from this research indicate that N. gonorrhoeae has a distinctive Cell Division interactome as compared with other microorganisms.
Yuh Nung Jan - One of the best experts on this subject based on the ideXlab platform.
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Asymmetric Cell Division.
Current opinion in cell biology, 2004Co-Authors: Fabrice Roegiers, Yuh Nung JanAbstract:Asymmetric Cell Division is a conserved mechanism for partitioning information during mitosis. Over the past several years, significant progress has been made in our understanding of how Cells establish polarity during asymmetric Cell Division and how determinants, in the form of localized proteins and mRNAs, are segregated. In particular, genetic studies in Drosophila and Caenorhabditis elegans have linked Cell polarity, G protein signaling and regulation of the cytoskeleton to coordination of mitotic spindle orientation and localization of determinants. Also, several new studies have furthered our understanding of how asymmetrically localized Cell fate determinants, such as the Numb, a negative regulator Notch signaling, functions in biasing Cell fates in the developing nervous system in Drosophila. In vertebrates, analysis of dividing neural progenitor Cells by in vivo imaging has raised questions about the role of asymmetric Cell Divisions during neurogenesis.
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Asymmetric Cell Division in the Drosophila nervous system.
Nature reviews. Neuroscience, 2001Co-Authors: Yuh Nung Jan, Lily Yeh JanAbstract:Asymmetric Cell Division is a fundamental means of generating Cell-fate diversity. Our understanding of the molecular mechanisms of asymmetric Cell Division in the Drosophila nervous system has advanced greatly in recent years, and insights gained from it might help to unravel the role of asymmetric Cell Division in the development of vertebrate nervous systems.
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asymmetric Cell Division
Nature, 1998Co-Authors: Yuh Nung Jan, Lily Yeh JanAbstract:With the recent identification of intrinsic Cell-fate determinants for asymmetric Cell Division in several systems, biologists have begun to gain insight into the Cellular mechanisms by which these determinants are preferentially segregated into one of the two daughter Cells during mitosis so that the daughter Cells acquire different fates.
Didier Gonze - One of the best experts on this subject based on the ideXlab platform.
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Modeling the effect of Cell Division on genetic oscillators.
Journal of theoretical biology, 2013Co-Authors: Didier GonzeAbstract:Many genetic oscillators (circadian clocks, synthetic oscillators) continue to oscillate across the Cell Division cycle. Since Cell Divisions create discontinuities in the dynamics of genetic oscillators the question about the resilience of oscillations and the factors that contribute to the robustness of the oscillations may be raised. We study here, through stochastic simulations, the effect of the Cell Division cycle on genetic oscillations using the Repressilator-a genetic oscillator developed in the context of synthetic biology. We consider intrinsic noise (molecular noise due to the limited number of molecules) and extrinsic noise (variability in the Cell Division time and in the partition of the molecules into daughter Cells, Cell-Cell variability in kinetic parameters, etc). Our numerical simulations show that, although noisy, oscillations are quite resilient to Cell Division and that Cell-Cell heterogeneity may be the main source of variability observed experimentally. Finally, similar simulations performed with another model, the Goodwin model, show that oscillations may be entrained and synchronized by Cell Division. This highlights the influence of the clock architecture on the robustness of genetic oscillations. Our approach provides a general framework to study the effect of Cell Division on dynamical systems and several possible extensions are described.
Jorge Z. Torres - One of the best experts on this subject based on the ideXlab platform.
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Phase Separation in Cell Division
Molecular cell, 2020Co-Authors: Joseph Y. Ong, Jorge Z. TorresAbstract:Summary Cell Division requires the assembly and organization of a microtubule spindle for the proper separation of chromosomes in mitosis and meiosis. Phase separation is an emerging paradigm for understanding spatial and temporal regulation of a variety of Cellular processes, including Cell Division. Phase-separated condensates have been recently discovered at many structures during Cell Division as a possible mechanism for properly localizing, organizing, and activating proteins involved in Cell Division. Here, we review how these condensates play roles in regulating microtubule density and organization and spindle assembly and function and in activating some of the key players in Cell Division. We conclude with perspectives on areas of future research for this exciting and rapidly advancing field.
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Dissecting the Mechanisms of Cell Division
The Journal of biological chemistry, 2019Co-Authors: Joseph Y. Ong, Jorge Z. TorresAbstract:Cell Division is a highly regulated and carefully orchestrated process. Understanding the mechanisms that promote proper Cell Division is an important step toward unraveling important questions in Cell biology and human health. Early studies seeking to dissect the mechanisms of Cell Division used classical genetics approaches to identify genes involved in mitosis and deployed biochemical approaches to isolate and identify proteins critical for Cell Division. These studies underscored that post-translational modifications and cyclin-kinase complexes play roles at the heart of the Cell Division program. Modern approaches for examining the mechanisms of Cell Division, including the use of high-throughput methods to study the effects of RNAi, cDNA, and chemical libraries, have evolved to encompass a larger biological and chemical space. Here, we outline some of the classical studies that established a foundation for the field and provide an overview of recent approaches that have advanced the study of Cell Division.