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Etheresia Pretorius - One of the best experts on this subject based on the ideXlab platform.

  • the stabilizing effect of an oligomeric proanthocyanidin on red blood Cell Membrane Structure of poorly controlled type ii diabetes
    Nutrition & Diabetes, 2017
    Co-Authors: J Visser, P Van Staden, Prashilla Soma, Antoinette V Buys, Etheresia Pretorius
    Abstract:

    Type II diabetes (T2D) is a pandemic characterized by pathological circulating inflammatory markers, high-glucose levels and oxidative stress. The hematological system is especially vulnerable to these aberrant circulating molecules, and erythrocytes (RBCs) show aberrant rheology properties, owing to the direct contact with these molecules. Pathological levels of circulating inflammatory markers in T2D therefore have a direct effect on the molecular and Cellular Structure of RBCs. Previous research has suggested that antioxidants may reduce oxidative stress that results from the pathological inflammatory markers. Particularly, polyphenol antioxidants like oligomeric proanthocyanidins (OPCs) may act as a hydroxyl mopping agent, and may have a positive effect on the deformability and Membrane protein Structure of RBCs from T2D. In this paper, we look at the effect of one such agent, Pinus massoniana bark extract (standardized to 95% oligomeric proanthicyanidins), on the RBC Membrane Structures and RBC shape changes of T2D, after laboratory exposure at physiological levels. Our methods of choice were atomic force microscopy and scanning electron microscopy to study RBC elasticity and ultraStructure. Results showed that in our hands, this OPC could change both the eryptotic nature of the RBCs, as viewed with scanning electron microscopy, as well as the elasticity. We found a significant difference in variation between the elasticity measurement values between the RBCs before and after OPC exposure (P-value <0.0001). In conclusion, the data from both these techniques therefore suggest that OPC usage might contribute to the improvement of RBC functioning.

  • The stabilizing effect of an oligomeric proanthocyanidin on red blood Cell Membrane Structure of poorly controlled Type II diabetes
    Nutrition & Diabetes, 2017
    Co-Authors: J Visser, Prashilla Soma, Antoinette V Buys, P J Van Staden, Etheresia Pretorius
    Abstract:

    Type II diabetes (T2D) is a pandemic characterized by pathological circulating inflammatory markers, high-glucose levels and oxidative stress. The hematological system is especially vulnerable to these aberrant circulating molecules, and erythrocytes (RBCs) show aberrant rheology properties, owing to the direct contact with these molecules. Pathological levels of circulating inflammatory markers in T2D therefore have a direct effect on the molecular and Cellular Structure of RBCs. Previous research has suggested that antioxidants may reduce oxidative stress that results from the pathological inflammatory markers. Particularly, polyphenol antioxidants like oligomeric proanthocyanidins (OPCs) may act as a hydroxyl mopping agent, and may have a positive effect on the deformability and Membrane protein Structure of RBCs from T2D. In this paper, we look at the effect of one such agent, Pinus massoniana bark extract (standardized to 95% oligomeric proanthicyanidins), on the RBC Membrane Structures and RBC shape changes of T2D, after laboratory exposure at physiological levels. Our methods of choice were atomic force microscopy and scanning electron microscopy to study RBC elasticity and ultraStructure. Results showed that in our hands, this OPC could change both the eryptotic nature of the RBCs, as viewed with scanning electron microscopy, as well as the elasticity. We found a significant difference in variation between the elasticity measurement values between the RBCs before and after OPC exposure ( P -value

  • changes in red blood Cell Membrane Structure in type 2 diabetes a scanning electron and atomic force microscopy study
    Cardiovascular Diabetology, 2013
    Co-Authors: Antoinette V Buys, Prashilla Soma, Miajean Van Rooy, Dirk Van Papendorp, Boguslaw Lipinski, Etheresia Pretorius
    Abstract:

    Red blood Cells (RBCs) are highly deformable and possess a robust Membrane that can withstand shear force. Previous research showed that in diabetic patients, there is a changed RBC ultraStructure, where these Cells are elongated and twist around spontaneously formed fibrin fibers. These changes may impact erythrocyte function. Ultrastructural analysis of RBCs in inflammatory and degenerative diseases can no longer be ignored and should form a fundamental research tool in clinical studies. Consequently, we investigated the Membrane roughness and ultrastructural changes in type 2 diabetes. Atomic force microscopy (AFM) was used to study Membrane roughness and we correlate this with scanning electron microscopy (SEM) to compare results of both the techniques with the RBCs of healthy individuals. We show that the combined AFM and SEM analyses of RBCs give valuable information about the disease status of patients with diabetes. Effectiveness of treatment regimes on the integrity, Cell shape and roughness of RBCs may be tracked, as this Cell’s health status is crucial to the overall wellness of the diabetic patient.

Antoinette V Buys - One of the best experts on this subject based on the ideXlab platform.

  • the stabilizing effect of an oligomeric proanthocyanidin on red blood Cell Membrane Structure of poorly controlled type ii diabetes
    Nutrition & Diabetes, 2017
    Co-Authors: J Visser, P Van Staden, Prashilla Soma, Antoinette V Buys, Etheresia Pretorius
    Abstract:

    Type II diabetes (T2D) is a pandemic characterized by pathological circulating inflammatory markers, high-glucose levels and oxidative stress. The hematological system is especially vulnerable to these aberrant circulating molecules, and erythrocytes (RBCs) show aberrant rheology properties, owing to the direct contact with these molecules. Pathological levels of circulating inflammatory markers in T2D therefore have a direct effect on the molecular and Cellular Structure of RBCs. Previous research has suggested that antioxidants may reduce oxidative stress that results from the pathological inflammatory markers. Particularly, polyphenol antioxidants like oligomeric proanthocyanidins (OPCs) may act as a hydroxyl mopping agent, and may have a positive effect on the deformability and Membrane protein Structure of RBCs from T2D. In this paper, we look at the effect of one such agent, Pinus massoniana bark extract (standardized to 95% oligomeric proanthicyanidins), on the RBC Membrane Structures and RBC shape changes of T2D, after laboratory exposure at physiological levels. Our methods of choice were atomic force microscopy and scanning electron microscopy to study RBC elasticity and ultraStructure. Results showed that in our hands, this OPC could change both the eryptotic nature of the RBCs, as viewed with scanning electron microscopy, as well as the elasticity. We found a significant difference in variation between the elasticity measurement values between the RBCs before and after OPC exposure (P-value <0.0001). In conclusion, the data from both these techniques therefore suggest that OPC usage might contribute to the improvement of RBC functioning.

  • The stabilizing effect of an oligomeric proanthocyanidin on red blood Cell Membrane Structure of poorly controlled Type II diabetes
    Nutrition & Diabetes, 2017
    Co-Authors: J Visser, Prashilla Soma, Antoinette V Buys, P J Van Staden, Etheresia Pretorius
    Abstract:

    Type II diabetes (T2D) is a pandemic characterized by pathological circulating inflammatory markers, high-glucose levels and oxidative stress. The hematological system is especially vulnerable to these aberrant circulating molecules, and erythrocytes (RBCs) show aberrant rheology properties, owing to the direct contact with these molecules. Pathological levels of circulating inflammatory markers in T2D therefore have a direct effect on the molecular and Cellular Structure of RBCs. Previous research has suggested that antioxidants may reduce oxidative stress that results from the pathological inflammatory markers. Particularly, polyphenol antioxidants like oligomeric proanthocyanidins (OPCs) may act as a hydroxyl mopping agent, and may have a positive effect on the deformability and Membrane protein Structure of RBCs from T2D. In this paper, we look at the effect of one such agent, Pinus massoniana bark extract (standardized to 95% oligomeric proanthicyanidins), on the RBC Membrane Structures and RBC shape changes of T2D, after laboratory exposure at physiological levels. Our methods of choice were atomic force microscopy and scanning electron microscopy to study RBC elasticity and ultraStructure. Results showed that in our hands, this OPC could change both the eryptotic nature of the RBCs, as viewed with scanning electron microscopy, as well as the elasticity. We found a significant difference in variation between the elasticity measurement values between the RBCs before and after OPC exposure ( P -value

  • changes in red blood Cell Membrane Structure in type 2 diabetes a scanning electron and atomic force microscopy study
    Cardiovascular Diabetology, 2013
    Co-Authors: Antoinette V Buys, Prashilla Soma, Miajean Van Rooy, Dirk Van Papendorp, Boguslaw Lipinski, Etheresia Pretorius
    Abstract:

    Red blood Cells (RBCs) are highly deformable and possess a robust Membrane that can withstand shear force. Previous research showed that in diabetic patients, there is a changed RBC ultraStructure, where these Cells are elongated and twist around spontaneously formed fibrin fibers. These changes may impact erythrocyte function. Ultrastructural analysis of RBCs in inflammatory and degenerative diseases can no longer be ignored and should form a fundamental research tool in clinical studies. Consequently, we investigated the Membrane roughness and ultrastructural changes in type 2 diabetes. Atomic force microscopy (AFM) was used to study Membrane roughness and we correlate this with scanning electron microscopy (SEM) to compare results of both the techniques with the RBCs of healthy individuals. We show that the combined AFM and SEM analyses of RBCs give valuable information about the disease status of patients with diabetes. Effectiveness of treatment regimes on the integrity, Cell shape and roughness of RBCs may be tracked, as this Cell’s health status is crucial to the overall wellness of the diabetic patient.

Prashilla Soma - One of the best experts on this subject based on the ideXlab platform.

  • the stabilizing effect of an oligomeric proanthocyanidin on red blood Cell Membrane Structure of poorly controlled type ii diabetes
    Nutrition & Diabetes, 2017
    Co-Authors: J Visser, P Van Staden, Prashilla Soma, Antoinette V Buys, Etheresia Pretorius
    Abstract:

    Type II diabetes (T2D) is a pandemic characterized by pathological circulating inflammatory markers, high-glucose levels and oxidative stress. The hematological system is especially vulnerable to these aberrant circulating molecules, and erythrocytes (RBCs) show aberrant rheology properties, owing to the direct contact with these molecules. Pathological levels of circulating inflammatory markers in T2D therefore have a direct effect on the molecular and Cellular Structure of RBCs. Previous research has suggested that antioxidants may reduce oxidative stress that results from the pathological inflammatory markers. Particularly, polyphenol antioxidants like oligomeric proanthocyanidins (OPCs) may act as a hydroxyl mopping agent, and may have a positive effect on the deformability and Membrane protein Structure of RBCs from T2D. In this paper, we look at the effect of one such agent, Pinus massoniana bark extract (standardized to 95% oligomeric proanthicyanidins), on the RBC Membrane Structures and RBC shape changes of T2D, after laboratory exposure at physiological levels. Our methods of choice were atomic force microscopy and scanning electron microscopy to study RBC elasticity and ultraStructure. Results showed that in our hands, this OPC could change both the eryptotic nature of the RBCs, as viewed with scanning electron microscopy, as well as the elasticity. We found a significant difference in variation between the elasticity measurement values between the RBCs before and after OPC exposure (P-value <0.0001). In conclusion, the data from both these techniques therefore suggest that OPC usage might contribute to the improvement of RBC functioning.

  • The stabilizing effect of an oligomeric proanthocyanidin on red blood Cell Membrane Structure of poorly controlled Type II diabetes
    Nutrition & Diabetes, 2017
    Co-Authors: J Visser, Prashilla Soma, Antoinette V Buys, P J Van Staden, Etheresia Pretorius
    Abstract:

    Type II diabetes (T2D) is a pandemic characterized by pathological circulating inflammatory markers, high-glucose levels and oxidative stress. The hematological system is especially vulnerable to these aberrant circulating molecules, and erythrocytes (RBCs) show aberrant rheology properties, owing to the direct contact with these molecules. Pathological levels of circulating inflammatory markers in T2D therefore have a direct effect on the molecular and Cellular Structure of RBCs. Previous research has suggested that antioxidants may reduce oxidative stress that results from the pathological inflammatory markers. Particularly, polyphenol antioxidants like oligomeric proanthocyanidins (OPCs) may act as a hydroxyl mopping agent, and may have a positive effect on the deformability and Membrane protein Structure of RBCs from T2D. In this paper, we look at the effect of one such agent, Pinus massoniana bark extract (standardized to 95% oligomeric proanthicyanidins), on the RBC Membrane Structures and RBC shape changes of T2D, after laboratory exposure at physiological levels. Our methods of choice were atomic force microscopy and scanning electron microscopy to study RBC elasticity and ultraStructure. Results showed that in our hands, this OPC could change both the eryptotic nature of the RBCs, as viewed with scanning electron microscopy, as well as the elasticity. We found a significant difference in variation between the elasticity measurement values between the RBCs before and after OPC exposure ( P -value

  • changes in red blood Cell Membrane Structure in type 2 diabetes a scanning electron and atomic force microscopy study
    Cardiovascular Diabetology, 2013
    Co-Authors: Antoinette V Buys, Prashilla Soma, Miajean Van Rooy, Dirk Van Papendorp, Boguslaw Lipinski, Etheresia Pretorius
    Abstract:

    Red blood Cells (RBCs) are highly deformable and possess a robust Membrane that can withstand shear force. Previous research showed that in diabetic patients, there is a changed RBC ultraStructure, where these Cells are elongated and twist around spontaneously formed fibrin fibers. These changes may impact erythrocyte function. Ultrastructural analysis of RBCs in inflammatory and degenerative diseases can no longer be ignored and should form a fundamental research tool in clinical studies. Consequently, we investigated the Membrane roughness and ultrastructural changes in type 2 diabetes. Atomic force microscopy (AFM) was used to study Membrane roughness and we correlate this with scanning electron microscopy (SEM) to compare results of both the techniques with the RBCs of healthy individuals. We show that the combined AFM and SEM analyses of RBCs give valuable information about the disease status of patients with diabetes. Effectiveness of treatment regimes on the integrity, Cell shape and roughness of RBCs may be tracked, as this Cell’s health status is crucial to the overall wellness of the diabetic patient.

J Visser - One of the best experts on this subject based on the ideXlab platform.

  • the stabilizing effect of an oligomeric proanthocyanidin on red blood Cell Membrane Structure of poorly controlled type ii diabetes
    Nutrition & Diabetes, 2017
    Co-Authors: J Visser, P Van Staden, Prashilla Soma, Antoinette V Buys, Etheresia Pretorius
    Abstract:

    Type II diabetes (T2D) is a pandemic characterized by pathological circulating inflammatory markers, high-glucose levels and oxidative stress. The hematological system is especially vulnerable to these aberrant circulating molecules, and erythrocytes (RBCs) show aberrant rheology properties, owing to the direct contact with these molecules. Pathological levels of circulating inflammatory markers in T2D therefore have a direct effect on the molecular and Cellular Structure of RBCs. Previous research has suggested that antioxidants may reduce oxidative stress that results from the pathological inflammatory markers. Particularly, polyphenol antioxidants like oligomeric proanthocyanidins (OPCs) may act as a hydroxyl mopping agent, and may have a positive effect on the deformability and Membrane protein Structure of RBCs from T2D. In this paper, we look at the effect of one such agent, Pinus massoniana bark extract (standardized to 95% oligomeric proanthicyanidins), on the RBC Membrane Structures and RBC shape changes of T2D, after laboratory exposure at physiological levels. Our methods of choice were atomic force microscopy and scanning electron microscopy to study RBC elasticity and ultraStructure. Results showed that in our hands, this OPC could change both the eryptotic nature of the RBCs, as viewed with scanning electron microscopy, as well as the elasticity. We found a significant difference in variation between the elasticity measurement values between the RBCs before and after OPC exposure (P-value <0.0001). In conclusion, the data from both these techniques therefore suggest that OPC usage might contribute to the improvement of RBC functioning.

  • The stabilizing effect of an oligomeric proanthocyanidin on red blood Cell Membrane Structure of poorly controlled Type II diabetes
    Nutrition & Diabetes, 2017
    Co-Authors: J Visser, Prashilla Soma, Antoinette V Buys, P J Van Staden, Etheresia Pretorius
    Abstract:

    Type II diabetes (T2D) is a pandemic characterized by pathological circulating inflammatory markers, high-glucose levels and oxidative stress. The hematological system is especially vulnerable to these aberrant circulating molecules, and erythrocytes (RBCs) show aberrant rheology properties, owing to the direct contact with these molecules. Pathological levels of circulating inflammatory markers in T2D therefore have a direct effect on the molecular and Cellular Structure of RBCs. Previous research has suggested that antioxidants may reduce oxidative stress that results from the pathological inflammatory markers. Particularly, polyphenol antioxidants like oligomeric proanthocyanidins (OPCs) may act as a hydroxyl mopping agent, and may have a positive effect on the deformability and Membrane protein Structure of RBCs from T2D. In this paper, we look at the effect of one such agent, Pinus massoniana bark extract (standardized to 95% oligomeric proanthicyanidins), on the RBC Membrane Structures and RBC shape changes of T2D, after laboratory exposure at physiological levels. Our methods of choice were atomic force microscopy and scanning electron microscopy to study RBC elasticity and ultraStructure. Results showed that in our hands, this OPC could change both the eryptotic nature of the RBCs, as viewed with scanning electron microscopy, as well as the elasticity. We found a significant difference in variation between the elasticity measurement values between the RBCs before and after OPC exposure ( P -value

Kazuhiko Ishihara - One of the best experts on this subject based on the ideXlab platform.

  • Cell Membrane inspired phospholipid polymers for developing medical devices with exCellent biointerfaces
    Science and Technology of Advanced Materials, 2012
    Co-Authors: Yasuhiko Iwasaki, Kazuhiko Ishihara
    Abstract:

    This review article describes fundamental aspects of Cell Membrane-inspired phospholipid polymers and their usefulness in the development of medical devices. Since the early 1990s, polymers composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) units have been considered in the preparation of biomaterials. MPC polymers can provide an artificial Cell Membrane Structure at the surface and serve as exCellent biointerfaces between artificial and biological systems. They have also been applied in the surface modification of some medical devices including long-term implantable artificial organs. An MPC polymer biointerface can suppress unfavorable biological reactions such as protein adsorption and Cell adhesion – in other words, specific biomolecules immobilized on an MPC polymer surface retain their original functions. MPC polymers are also being increasingly used for creating biointerfaces with artificial Cell Membrane Structures.

  • integrated functional nanocolloids covered with artificial Cell Membranes for biomedical applications
    Nano Today, 2011
    Co-Authors: Ryosuke Matsuno, Kazuhiko Ishihara
    Abstract:

    Summary The functionality of nanocolloids used in biomedical applications are subject to strong interference arising from significant interactions with biological components such as proteins and Cells. Among the known examples of surface treatment of nanocolloids, the construction of an artificial Cell Membrane Structure based on phospholipid polymers has proven effective in preventing the occurrence of biological reactions at the surface. Furthermore, certain bioactive molecules can be immobilized on the surface of the phospholipid polymer platform to generate bioaffinity for other biomolecules. This review describes preparation and characterization of integrated functional nanocolloids covered by artificial Cell Membrane Structures and their performance in biomedical applications.

  • Novel polymer biomaterials and interfaces inspired from Cell Membrane functions.
    Biochimica et Biophysica Acta, 2010
    Co-Authors: Kazuhiko Ishihara, Yuuki Inoue, Yusuke Goto, Madoka Takai, Ryosuke Matsuno, Tomohiro Konno
    Abstract:

    Abstract Background Materials with exCellent biocompatibility on interfaces between artificial system and biological system are needed to develop any equipments and devices in bioscience, bioengineering and medicinal science. Suppression of unfavorable biological response on the interface is most important for understanding real functions of biomolecules on the surface. So, we should design and prepare such biomaterials. Scoop of review One of the best ways to design the biomaterials is generated from mimicking a Cell Membrane Structure. It is composed of a phospholipid bilayered Membrane and embedded proteins and polysaccharides. The surface of the Cell Membrane-like Structure is constructed artificially by molecular integration of phospholipid polymer as platform and conjugated biomolecules. Here, it is introduced as the effectiveness of biointerface with highly biological functions observed on artificial Cell Membrane Structure. Major conclusions Reduction of nonspecific protein adsorption is essential for suppression of unfavorable bioresponse and achievement of versatile biomedical applications. Simultaneously, bioconjugation of biomolecules on the phospholipid polymer platform is crucial for a high-performance interface. General significance The biointerfaces with both biocompatibility and biofunctionality based on biomolecules must be installed on advanced devices, which are applied in the fields of nanobioscience and nanomedicine. This article is part of a Special Issue entitled Nanotechnologies - Emerging Applications in Biomedicine.

  • Fabrication of a Cell-adhesive protein imprinting surface with an artificial Cell Membrane Structure for Cell capturing.
    Biosensors & bioelectronics, 2009
    Co-Authors: Kyoko Fukazawa, Kazuhiko Ishihara
    Abstract:

    Abstract We proposed a new molecular imprinting procedure based on molecular integration for the purpose of Cell capture. We selected the Cell-adhesive protein fibronectin (FN) as the imprinting protein for preparing templates and evaluated selective Cell adhesion on the FN imprinting substrate. Silica beads with a diameter of 15 μm were used as the stamp matrix and FN molecules were adsorbed as a monolayer. The FN recognition sites were constructed by integrating a surfactant as the ligand and immobilizing it with new biocompatible photoreactive phospholipid polymer composed of 2-methacryloyloxyethyl phosphorylcholine (MPC) units. As control substrates, imprinting procedures were carried out using albumin (BSA imprinting substrate) and without imprinting protein (non-imprinting substrate). The binding of FN from the Cell culture medium with the fetal calf serum was achieved on the FN imprinting substrate, and induced the Cell adhesion. On the other hand, on the non-imprinted and BSA imprinting substrates, the FN scarcely bound from the Cell culture medium, and subsequent Cell adhesion could not be observed on the substrate. These results indicate that the FN binding sites were well constructed by arranging the ligand surfactant to a suitable position and immobilized by the photoreactive MPC polymer. The MPC polymer prevented the nonspecific adsorption of proteins from the Cell culture medium. We concluded that this procedure is convenient and can be potentially used for the preparation of surfaces for Cell engineering devices.