The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Qizhi Chen - One of the best experts on this subject based on the ideXlab platform.
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biomaterials in cardiac tissue engineering ten years of research survey
Materials Science & Engineering R-reports, 2008Co-Authors: Qizhi Chen, Siân E Harding, Alexander R Lyon, Aldo R BoccacciniAbstract:Driven by enormous clinical need, myocardial tissue engineering has become a prime focus of research within the field of tissue engineering. Myocardial tissue engineering combines isolated functional cardiomyocytes and a biodegradable or nondegradable biomaterial to repair diseased heart muscle. The challenges in heart muscle engineering include Cell related issues (such as scale up in a short timeframe, efficiency of Cell seeding or Cell Survival Rate, and immune rejection), the design and fabrication of myocardial tissue engineering substRates, and the engineering of tissue constructs in vitro and in vivo. Several approaches have been put forward, and a number of models combining various polymeric biomaterials, Cell sources and bioreactors have been developed in the last 10 years for myocardial tissue engineering. This review provides a comprehensive update on the biomaterials, as well as Cells and biomimetic systems, used in the engineering of the cardiac muscle. The article is organized as follows. A historic perspective of the evolution of cardiac medicine and emergence of cardiac tissue engineering is presented in the first section. Following a review on the Cells used in myocardial tissue engineering (second section), the third section presents a review on biomaterials used in myocardial tissue engineering. This section starts with an overview of the development of tissue engineering substRates and goes on to discuss the selection of biomaterials and design of solid and porous substRates. Then the applications of a variety of biomaterials used in different approaches of myocardial tissue engineering are reviewed in great detail, and related issues and topics that remain challenges for the future progress of the field are identified at the end of each subsection. This is followed by a brief review on the development of bioreactors (fourth section), which is an important achievement in the field of myocardial tissue engineering, and which is also related to the biomaterials developed. At the end of this article, the major achievements and remaining challenges are summarized, and the most promising paradigm for the future of heart muscle tissue engineering is proposed (fifth section).
Lv Che - One of the best experts on this subject based on the ideXlab platform.
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an ultrasonic nanobubble mediated pnp fludarabine suicide gene system a new approach for the treatment of hepatoCellular carcinoma
PLOS ONE, 2018Co-Authors: O Zhang, Youming Zhang, Lihua Zhang, Mingna Che, Wei Che, Lv CheAbstract:Objective The purpose of this study is to geneRate an ultrasonic nanobubble (NB)-mediated purine nucleoside phosphorylase (PNP)/fludarabine suicide gene system for the treatment of human hepatoCellular carcinoma (HCC). Methods NBs were prepared from a mixture the phospholipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphate (DPPA), perfluoropropane gas and other materials using the high shear dispersion method. NBs treated with ultrasound irradiation functioned as a gene-transfer system, and a self-constructed suicide gene expression plasmid, pcDNA3.1(+)/PNP, treated with fludarabine functioned as a therapeutic gene. This system was used to determine the cytotoxic effects of PNP/fludarabine on HepG2 Cells and SMMC7721 Cells. Results 1. NBs with a small diameter (208–416 nm) and at a high concentration and fine homogeneity were prepared under the optimal method. 2. The pcDNA3.1(+)/PNP plasmid was efficiently transfected into HCC Cells using ultrasonic NBs. 3. At 0.75μg/ml fludarabine, PNP/fludarabine showed marked cytotoxic effects toward HepG2 and SMMC7721 Cells. PNP/fludarabine achieved the same effect against both SMMC7721 and HepG2 Cells but at a lower concentration of fludarabine for the latter. 4. Bystander effects: a 10–20% decrease in the Cell Survival Rate was observed when only 5–10% of transfected Cells were PNP positive. Conclusions NBs constitute a non-toxic, stable and effective gene-delivery platform. The PNP/fludarabine suicide gene system inhibited the growth of HCC Cells, induced HCC Cell apoptosis, and caused a notable bystander effect at a low fludarabine concentration. This study establishes an important new method for miniaturizing microbubbles and improving a new NB-mediated approach for gene therapy of HCC.
Fenghuei Lin - One of the best experts on this subject based on the ideXlab platform.
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preparation and in vitro evaluation of fe2o3 doped dp bioglass in combination with 3d printing and selective laser sintering process 3dp sls for alveolar bone augmentation
Ceramics International, 2021Co-Authors: Chihying Chi, Chingyun Chen, Jianyuan Huang, Cheyung Kuan, Yuying Lin, Chunchen Yang, Fenghuei LinAbstract:Abstract Severe periodontal disease can cause damage and atrophy of alveolar bone. Presently, Fe2O3-doped DP-bioglass (DPF-bioglass) was prepared and combined with a 3D-printing and selective laser sintering (3DP-SLS) process to prepare a porous scaffold for alveolar bone augmentation of dental implants. Addition of 2% Fe2O3 effectively lowered the melting point and darkened the color to absorb the laser energy and increased the sintering efficiency. X-ray diffraction, optical microscopy (OM), energy dispersive spectrophotometry, and differential thermal analysis were used to characterize the crystal structure, color/darkness, morphology, qualitative chemical composition, and thermal stability, respectively, for the synthesized DPF-bioglass. After the human fetal osteoblasts (hFOB 1.19 Cells) were cultured with the extraction medium, Cell morphology was observed by OM. The WST-1 and lactate dehydrogenase (LDH) assays were used to evaluate the cytotoxicity of the DPF-bioglass. This bioglass was then prepared as an alveolar bone substitute (ABS) by the 3DP-SLS process and Cells were cultured on the scaffold. The Cell morphology was revealed by scanning electron microscopy (SEM). Cell Survival Rate and Cells in early apoptosis were examined using live/dead and JC-1 staining, respectively. The gene expression of Runx2, type I collagen, and alkaline phosphatase (ALP) were analyzed by qPCR to check early osteogenesis, extraCellular matrix secretion, and mineralization, respectively. Xylenol orange (XO) staining was used to observe the mineralization of calcium phosphate deposition. The improvements in Cell attachment, proliferation, and biomineralization were further confirmed in terms of potential bone regeneration in vitro. The developed ABS was not cytotoxic to human osteoblasts in the WST-1, LDH, live/dead and JC-1 stain. The developed ABS gradually degraded and constantly released Ca+2, PO4−3, Fe+3, and Si+4 in the physiological environment. SEM and XO staining revealed that the released ions promoted bone formation and mineralization. Osteogenesis was also enhanced, as judged by early induction of the gene expression.
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a highly organized three dimensional alginate scaffold for cartilage tissue engineering prepared by microfluidic technology
Biomaterials, 2011Co-Authors: Chen Chie Wang, Hwa-chang Liu, Kai Chiang Yang, Kenghui Lin, Fenghuei LinAbstract:Osteoarthritis is a degenerative disease and frequently involves the knee, hip and phalangeal joints. Current treatments used in small cartilage defects including multiple drilling, abrasion arthroplasty, mosaicplasty, and autogenous chondrocyte transplantation, however, there are problems needed to be solved. The standard treatment for severe osteoarthritis is total joint arthroplasty. The disadvantages of this surgery are the possibility of implant loosening. Therefore, tissue engineering for cartilage regeneration has become a promising topic. We have developed a new method to produce a highly organized single polymer (alginate) scaffold using microfluidic device. Scanning electron microscope and confocal fluoroscope examinations showed that the scaffold has a regular interconnected porous structure in the scale of 250 μm and high porosity. The scaffold is effective in chondrocyte culture; the Cell viability test (WST-1 assay), Cell toxicity (lactate dehydrogenase assay), Cell Survival Rate, extraCellular matrix production (glycosaminoglycans contents), Cell proliferation (DNA quantification), and gene expression (real-time PCR) all revealed good results for chondrocyte culture. The chondrocytes can maintain normal phenotypes, highly express aggrecan and type II collagen, and secrete a great deal of extraCellular matrix when seeded in the alginate scaffold. This study demonstRated that a highly organized alginate scaffold can be prepared with an economical microfluidic device, and this scaffold is effective in cartilage tissue engineering.
Aldo R Boccaccini - One of the best experts on this subject based on the ideXlab platform.
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biomaterials in cardiac tissue engineering ten years of research survey
Materials Science & Engineering R-reports, 2008Co-Authors: Qizhi Chen, Siân E Harding, Alexander R Lyon, Aldo R BoccacciniAbstract:Driven by enormous clinical need, myocardial tissue engineering has become a prime focus of research within the field of tissue engineering. Myocardial tissue engineering combines isolated functional cardiomyocytes and a biodegradable or nondegradable biomaterial to repair diseased heart muscle. The challenges in heart muscle engineering include Cell related issues (such as scale up in a short timeframe, efficiency of Cell seeding or Cell Survival Rate, and immune rejection), the design and fabrication of myocardial tissue engineering substRates, and the engineering of tissue constructs in vitro and in vivo. Several approaches have been put forward, and a number of models combining various polymeric biomaterials, Cell sources and bioreactors have been developed in the last 10 years for myocardial tissue engineering. This review provides a comprehensive update on the biomaterials, as well as Cells and biomimetic systems, used in the engineering of the cardiac muscle. The article is organized as follows. A historic perspective of the evolution of cardiac medicine and emergence of cardiac tissue engineering is presented in the first section. Following a review on the Cells used in myocardial tissue engineering (second section), the third section presents a review on biomaterials used in myocardial tissue engineering. This section starts with an overview of the development of tissue engineering substRates and goes on to discuss the selection of biomaterials and design of solid and porous substRates. Then the applications of a variety of biomaterials used in different approaches of myocardial tissue engineering are reviewed in great detail, and related issues and topics that remain challenges for the future progress of the field are identified at the end of each subsection. This is followed by a brief review on the development of bioreactors (fourth section), which is an important achievement in the field of myocardial tissue engineering, and which is also related to the biomaterials developed. At the end of this article, the major achievements and remaining challenges are summarized, and the most promising paradigm for the future of heart muscle tissue engineering is proposed (fifth section).
Qiang Dong - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Neuroprotective Effect of Neuroserpin in Oxygen-Glucose Deprivation- and Reoxygenation-Treated Rat Astrocytes In
2016Co-Authors: Yang Zhang, Tetsuya Asakawa, Sha Han, Baoguo Xiao, Hiroki Namba, Qiang DongAbstract:Neuroserpin (NSP) reportedly exerts neuroprotective effects in cerebral ischemic animal models and patients; however, the mechanism of protection is poorly understood. We thus attempted to confirm neuroprotective effects of NSP on astrocytes in the ischemic state and then explored the relative mechanisms. Astrocytes from neonatal rats were treated with oxygen-glucose deprivation (OGD) followed by reoxygenation (OGD/R). To confirm the neuroprotective effects of NSP, we measured the Cell Survival Rate, relative lactate dehydro-genase (LDH) release; we also performed morphological methods, namely Hoechst 33342 staining and Annexin V assay. To explore the potential mechanisms of NSP, the release of nitric oxide (NO) and TNF-α related to NSP administration were measured by enzyme-linked immunosorbent assay. The proteins related to the NF-κB, ERK1/2, and PI3K/Akt pathways were investigated by Western blotting. To verify the cause-and-effect relationship between neuroprotection and the NF-κB pathway, a NF-κB pathway inhibitor sc3060 was employed to observe the effects of NSP-induced neuroprotection. We found that NSP sig-nificantly increased the Cell Survival Rate and reduced LDH release in OGD/R-treated astro
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neuroprotective effect of neuroserpin in oxygen glucose deprivation and reoxygenation treated rat astrocytes in vitro
PLOS ONE, 2015Co-Authors: Liang Wang, Yang Zhang, Tetsuya Asakawa, Sha Han, Baoguo Xiao, Hiroki Namba, Qiang DongAbstract:Neuroserpin (NSP) reportedly exerts neuroprotective effects in cerebral ischemic animal models and patients; however, the mechanism of protection is poorly understood. We thus attempted to confirm neuroprotective effects of NSP on astrocytes in the ischemic state and then explored the relative mechanisms. Astrocytes from neonatal rats were treated with oxygen-glucose deprivation (OGD) followed by reoxygenation (OGD/R). To confirm the neuroprotective effects of NSP, we measured the Cell Survival Rate, relative lactate dehydrogenase (LDH) release; we also performed morphological methods, namely Hoechst 33342 staining and Annexin V assay. To explore the potential mechanisms of NSP, the release of nitric oxide (NO) and TNF-α related to NSP administration were measured by enzyme-linked immunosorbent assay. The proteins related to the NF-κB, ERK1/2, and PI3K/Akt pathways were investigated by Western blotting. To verify the cause-and-effect relationship between neuroprotection and the NF-κB pathway, a NF-κB pathway inhibitor sc3060 was employed to observe the effects of NSP-induced neuroprotection. We found that NSP significantly increased the Cell Survival Rate and reduced LDH release in OGD/R-treated astrocytes. It also reduced NO/TNF-α release. Western blotting showed that the protein levels of p-IKKBα/β and P65 were upregulated by the OGD/R treatment and such effects were significantly inhibited by NSP administration. The NSP-induced inhibition could be significantly reversed by administration of the NF-κB pathway inhibitor sc3060, whereas, expressions of p-ERK1, p-ERK2, and p-AKT were upregulated by the OGD/R treatment; however, their levels were unchanged by NSP administration. Our results thus verified the neuroprotective effects of NSP in ischemic astrocytes. The potential mechanisms include inhibition of the release of NO/TNF-α and repression of the NF-κB signaling pathways. Our data also indicated that NSP has little influence on the MAPK and PI3K/Akt pathways.