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Daniel Kost - One of the best experts on this subject based on the ideXlab platform.
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Ultraviolet radiation induces Stress in etiolated Landoltia punctata, as evidenced by the presence of alanine, a universal Stress Signal: a 15n NMR study.
Plant Biology, 2014Co-Authors: Edna Ben-izhak Monselise, A. Levkovitz, Daniel KostAbstract:Analysis with 15N NMR revealed that alanine, a universal Cellular Stress Signal, accumulates in etiolated duckweed plants exposed to 15-min pulsed UV light, but not in the absence of UV irradiation. The addition of 10 mm vitamin C, a radical scavenger, reduced alanine levels to zero, indicating the involvement of free radicals. Free D-alanine was detected in 15N NMR analysis of the chiral amino acid content, using D-tartaric acid as solvent. The accumulation of D-alanine under Stress conditions presents a new perspective on the biochemical processes taking place in prokaryote and eukaryote cells.
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Ultraviolet radiation induces Stress in etiolated Landoltia punctata, as evidenced by the presence of alanine, a universal Stress Signal: a ¹⁵N NMR study.
Plant biology (Stuttgart Germany), 2014Co-Authors: Edna Ben-izhak Monselise, A. Levkovitz, Daniel KostAbstract:Analysis with (15) N NMR revealed that alanine, a universal Cellular Stress Signal, accumulates in etiolated duckweed plants exposed to 15-min pulsed UV light, but not in the absence of UV irradiation. The addition of 10 mm vitamin C, a radical scavenger, reduced alanine levels to zero, indicating the involvement of free radicals. Free D-alanine was detected in (15) N NMR analysis of the chiral amino acid content, using D-tartaric acid as solvent. The accumulation of D-alanine under Stress conditions presents a new perspective on the biochemical processes taking place in prokaryote and eukaryote cells.
Edna Ben-izhak Monselise - One of the best experts on this subject based on the ideXlab platform.
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Ultraviolet radiation induces Stress in etiolated Landoltia punctata, as evidenced by the presence of alanine, a universal Stress Signal: a 15n NMR study.
Plant Biology, 2014Co-Authors: Edna Ben-izhak Monselise, A. Levkovitz, Daniel KostAbstract:Analysis with 15N NMR revealed that alanine, a universal Cellular Stress Signal, accumulates in etiolated duckweed plants exposed to 15-min pulsed UV light, but not in the absence of UV irradiation. The addition of 10 mm vitamin C, a radical scavenger, reduced alanine levels to zero, indicating the involvement of free radicals. Free D-alanine was detected in 15N NMR analysis of the chiral amino acid content, using D-tartaric acid as solvent. The accumulation of D-alanine under Stress conditions presents a new perspective on the biochemical processes taking place in prokaryote and eukaryote cells.
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Ultraviolet radiation induces Stress in etiolated Landoltia punctata, as evidenced by the presence of alanine, a universal Stress Signal: a ¹⁵N NMR study.
Plant biology (Stuttgart Germany), 2014Co-Authors: Edna Ben-izhak Monselise, A. Levkovitz, Daniel KostAbstract:Analysis with (15) N NMR revealed that alanine, a universal Cellular Stress Signal, accumulates in etiolated duckweed plants exposed to 15-min pulsed UV light, but not in the absence of UV irradiation. The addition of 10 mm vitamin C, a radical scavenger, reduced alanine levels to zero, indicating the involvement of free radicals. Free D-alanine was detected in (15) N NMR analysis of the chiral amino acid content, using D-tartaric acid as solvent. The accumulation of D-alanine under Stress conditions presents a new perspective on the biochemical processes taking place in prokaryote and eukaryote cells.
A. Levkovitz - One of the best experts on this subject based on the ideXlab platform.
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Ultraviolet radiation induces Stress in etiolated Landoltia punctata, as evidenced by the presence of alanine, a universal Stress Signal: a 15n NMR study.
Plant Biology, 2014Co-Authors: Edna Ben-izhak Monselise, A. Levkovitz, Daniel KostAbstract:Analysis with 15N NMR revealed that alanine, a universal Cellular Stress Signal, accumulates in etiolated duckweed plants exposed to 15-min pulsed UV light, but not in the absence of UV irradiation. The addition of 10 mm vitamin C, a radical scavenger, reduced alanine levels to zero, indicating the involvement of free radicals. Free D-alanine was detected in 15N NMR analysis of the chiral amino acid content, using D-tartaric acid as solvent. The accumulation of D-alanine under Stress conditions presents a new perspective on the biochemical processes taking place in prokaryote and eukaryote cells.
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Ultraviolet radiation induces Stress in etiolated Landoltia punctata, as evidenced by the presence of alanine, a universal Stress Signal: a ¹⁵N NMR study.
Plant biology (Stuttgart Germany), 2014Co-Authors: Edna Ben-izhak Monselise, A. Levkovitz, Daniel KostAbstract:Analysis with (15) N NMR revealed that alanine, a universal Cellular Stress Signal, accumulates in etiolated duckweed plants exposed to 15-min pulsed UV light, but not in the absence of UV irradiation. The addition of 10 mm vitamin C, a radical scavenger, reduced alanine levels to zero, indicating the involvement of free radicals. Free D-alanine was detected in (15) N NMR analysis of the chiral amino acid content, using D-tartaric acid as solvent. The accumulation of D-alanine under Stress conditions presents a new perspective on the biochemical processes taking place in prokaryote and eukaryote cells.
Helmut Ruis - One of the best experts on this subject based on the ideXlab platform.
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Stress factors acting at the level of the plasma membrane induce transcription via the Stress response element (STRE) of the yeast Saccharomyces cerevisiae.
Molecular microbiology, 1999Co-Authors: Eugenia Moskvina, Esther-maria Imre, Helmut RuisAbstract:A variety of Stress factors induces transcription via the Stress response element (STRE) present in control regions of a number of genes of the yeast Saccharomyces cerevisiae. Induction of transcription involves nuclear translocation of the STRE-binding transcription activators Msn2p and Msn4p. The primary Cellular events triggering this translocation are presently not well understood. In this investigation, we have observed that a number of factors acting at the level of the yeast plasma membrane, including the antifungal agent nystatin, the steroidal alkaloid tomatine, benzyl alcohol, a number of detergents and the plasma membrane H+-ATPase inhibitor diethylstilbestrol or mutations in the PMA1 gene encoding the plasma membrane ATPase, induce Msn2p nuclear accumulation and STRE-dependent transcription. At least some of the Stress factors acting via STREs cause an increase in plasma membrane permeability, leading to a decrease in membrane potential, which might be a primary Cellular Stress Signal. A decrease in internal pH triggered by permeabilization of the plasma membrane or a change in cAMP levels are at least not obligatory factors in intraCellular Stress Signal transduction. The Signal transduction pathway transmitting the Signal generated at the plasma membrane to Msn2p is still unknown.
Marie Adeline Marques - One of the best experts on this subject based on the ideXlab platform.
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Growth and differentiation factor 15 is secreted by skeletal muscle during exercise and promotes lipolysis in humans.
JCI insight, 2020Co-Authors: Claire Laurens, Anisha Parmar, Enda Murphy, Deborah Carper, Benjamin Lair, Pauline Maes, Julie Vion, Nathalie Boulet, Coralie Fontaine, Marie Adeline MarquesAbstract:We hypothesized that skeletal muscle contraction produces a Cellular Stress Signal, triggering adipose tissue lipolysis to sustain fuel availability during exercise. The present study aimed at identifying exercise-regulated myokines, also known as exerkines, able to promote lipolysis. Human primary myotubes from lean healthy volunteers were submitted to electrical pulse stimulation (EPS) to mimic either acute intense or chronic moderate exercise. Conditioned media (CM) experiments with human adipocytes were performed. CM and human plasma samples were analyzed using unbiased proteomic screening and/or ELISA. Real-time qPCR was performed in cultured myotubes and muscle biopsy samples. CM from both acute intense and chronic moderate exercise increased basal lipolysis in human adipocytes. Growth and differentiation factor 15 (GDF15) gene expression and secretion increased rapidly upon skeletal muscle contraction. GDF15 protein was upregulated in CM from both acute and chronic exercise-stimulated myotubes. We further showed that physiological concentrations of recombinant GDF15 protein increased lipolysis in human adipose tissue, while blocking GDF15 with a neutralizing antibody abrogated EPS CM-mediated lipolysis. We herein provide the first evidence to our knowledge that GDF15 is a potentially novel exerkine produced by skeletal muscle contraction and able to target human adipose tissue to promote lipolysis.