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Kitti Jirarattanaphochai - One of the best experts on this subject based on the ideXlab platform.
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prevention of peridural fibrosis using a cyclooxygenase 2 inhibitor nonsteroidal anti inflammatory drug soaked in absorbable gelatin sponge an experimental comparative animal model
Spine, 2013Co-Authors: Surachai Saejung, Kitti JirarattanaphochaiAbstract:Abstract Experimental study. To evaluate the efficacy and safety of peridural parecoxib-soaked absorbable gelatin sponge, and Cellulose Membrane on peridural fibrosis prevention in an animal model. Postoperative peridural fibrosis is one of the causes of failed back surgery syndrome. Nonsteroidal anti-inflammatory drugs inhibit the inflammatory response, while an absorbable gelatin sponge or Cellulose Membrane interposes between the dura and the paraspinal muscle to staunch the surgical bleeding. These mechanisms may prevent peridural fibrosis. Forty L5-L6 laminectomized adult Sprague-Dawley rats were randomly allocated into 4 groups. The high parecoxib group received 6 mg of parecoxib soaked into an absorbable gelatin sponge placed over the dura. The low parecoxib group was given 2 mg of parecoxib soaked into an absorbable gelatin sponge. The dura in the Cellulose group was covered with a Cellulose Membrane, while the control group received normal saline drip before surgical wound closure. All rats were killed at 6 weeks for histopathological assessment. The fibroblast density, inflammatory cell density, fibrous adherence, and adverse events were quantified. The obtained results were analyzed statistically. The respective mean fibroblast density in the high parecoxib, low parecoxib, Cellulose, and control groups was 217.77 ± 51.76, 317.51 ± 126.92, 321.80 ± 90.94, and 328.48 ± 73.41 cells/mm², while the respective mean inflammatory cell density was 539.65 ± 236.52, 910.17 ± 242.59, 1011.84 ± 239.30, and 1261.78 ± 319.68 cells/mm². The mean fibroblast and inflammatory cell densities of the high parecoxib group were significantly lower than the control. The high parecoxib group also showed statistically less fibrous adherence than low parecoxib, Cellulose, and control groups. The high-dose parecoxib-soaked absorbable gelatin sponge can prevent peridural fibrosis without complications. The low-dose parecoxib and Cellulose Membrane provided no significant benefit vis-a-vis prevention of peridural fibrosis, as adduced from the lack of any statistically significant difference between the test and control rats.
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prevention of peridural fibrosis using a cyclooxygenase 2 inhibitor nonsteroidal anti inflammatory drug soaked in absorbable gelatin sponge an experimental comparative animal model
Spine, 2013Co-Authors: Surachai Saejung, Kitti JirarattanaphochaiAbstract:Study design Experimental study. Objective To evaluate the efficacy and safety of peridural parecoxib-soaked absorbable gelatin sponge, and Cellulose Membrane on peridural fibrosis prevention in an animal model. Summary of background data Postoperative peridural fibrosis is one of the causes of failed back surgery syndrome. Nonsteroidal anti-inflammatory drugs inhibit the inflammatory response, while an absorbable gelatin sponge or Cellulose Membrane interposes between the dura and the paraspinal muscle to staunch the surgical bleeding. These mechanisms may prevent peridural fibrosis. Methods Forty L5-L6 laminectomized adult Sprague-Dawley rats were randomly allocated into 4 groups. The high parecoxib group received 6 mg of parecoxib soaked into an absorbable gelatin sponge placed over the dura. The low parecoxib group was given 2 mg of parecoxib soaked into an absorbable gelatin sponge. The dura in the Cellulose group was covered with a Cellulose Membrane, while the control group received normal saline drip before surgical wound closure. All rats were killed at 6 weeks for histopathological assessment. The fibroblast density, inflammatory cell density, fibrous adherence, and adverse events were quantified. The obtained results were analyzed statistically. Results The respective mean fibroblast density in the high parecoxib, low parecoxib, Cellulose, and control groups was 217.77 ± 51.76, 317.51 ± 126.92, 321.80 ± 90.94, and 328.48 ± 73.41 cells/mm², while the respective mean inflammatory cell density was 539.65 ± 236.52, 910.17 ± 242.59, 1011.84 ± 239.30, and 1261.78 ± 319.68 cells/mm². The mean fibroblast and inflammatory cell densities of the high parecoxib group were significantly lower than the control. The high parecoxib group also showed statistically less fibrous adherence than low parecoxib, Cellulose, and control groups. Conclusion The high-dose parecoxib-soaked absorbable gelatin sponge can prevent peridural fibrosis without complications. The low-dose parecoxib and Cellulose Membrane provided no significant benefit vis-a-vis prevention of peridural fibrosis, as adduced from the lack of any statistically significant difference between the test and control rats.
Udo Reichl - One of the best experts on this subject based on the ideXlab platform.
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use of sulfated Cellulose Membrane adsorbers for chromatographic purification of cell cultured derived influenza a and b viruses
Separation and Purification Technology, 2019Co-Authors: Raquel A Fortuna, Louis Villain, Sebastian Van Teeffelen, Adrian Ley, Laura M Fischer, Florian Taft, Yvonne Genzel, Michael W Wolff, Udo ReichlAbstract:Abstract Influenza virus particles can be purified by pseudo-affinity chromatography using sulfated carbohydrate matrices. In this study, we compared the binding capacity and the purification performance of two bead-based resins and one Membrane adsorber for three influenza virus strains (H1N1, H3N2 and B) produced in MDCK suspension cells in a chemically defined medium. The dynamic binding capacity for the sulfated Cellulose Membrane adsorbers was consistently higher than for the resins (8 to 22-fold). Confocal microscopy demonstrated, that while virus particles uniformly covered the complete surface of the Membrane, the virus particles only bound to the external surface of the sulfated beads. Overall, recovery of virus varied between 66% and 81%. Total protein and DNA removal were >74% and >96%, respectively. Statistically significant differences in the purification performance were more often observed between strains than between matrices. In particular, H3N2 influenza virus particles could only be purified using the sulfated Membrane adsorbers. Due to the higher operating flow rate and binding capacity, the productivity with the Membrane adsorbers was on average 25-times higher than with the resins.
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purification of influenza virus like particles using sulfated Cellulose Membrane adsorbers
Journal of Chemical Technology & Biotechnology, 2018Co-Authors: Sofia B Carvalho, Raquel A Fortuna, Michael W Wolff, Udo Reichl, Cristina Peixoto, Paula M Alves, Manuel J T CarrondoAbstract:BACKGROUND Vaccines based on virus-like particles (VLPs) are an alternative to inactivated viral vaccines that combine good safety profiles with strong immunogenicity. In order to be economically competitive, efficient manufacturing is required, in particular downstream processing, which often accounts for major production costs. This study describes the optimization and establishment of a chromatography capturing technique using sulfated Cellulose Membrane adsorbers (SCMA) for purification of influenza VLPs. RESULTS Using a design of experiments approach, the critical factors for SCMA performance were described and optimized. For optimal conditions (Membrane ligand density: 15.4 µmol cm-2, salt concentration of the loading buffer: 24 mmol L-1 NaCl, and elution buffer: 920 mmol L-1 NaCl, as well as the corresponding flow rates: 0.24 and 1.4 mL min-1), a yield of 80% in the product fraction was obtained. No loss of VLPs was detected in the flowthrough fraction. Removal of total protein and DNA impurities were higher than 89% and 80%, respectively. CONCLUSION Use of SCMA represents a significant improvement compared with conventional ion exchanger Membrane adsorbers. As the method proposed is easily scalable and reduces the number of steps required compared with conventional purification methods, SCMA could qualify as a generic platform for purification of VLP-based influenza vaccines. © 2017 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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optimization of cell culture derived influenza a virus particles purification using sulfated Cellulose Membrane adsorbers
Engineering in Life Sciences, 2018Co-Authors: Ana Raquel Fortuna, Louis Villain, Florian Taft, Michael W Wolff, Udo ReichlAbstract:Downstream processing remains one of the biggest challenges in manufacturing of biologicals and vaccines. This work focuses on a Design of Experiments approach to understand factors influencing the performance of sulfated Cellulose Membrane adsorbers for the chromatographic purification of a cell culture-derived H1N1 influenza virus strain (A/Puerto Rico/8/34). Membranes with a medium ligand density together with low conductivity and a high virus titer in the feed stream resulted in optimum virus yields and low protein and DNA content in the product fraction. Flow rate and salt concentration in the buffer used for elution were of secondary importance while Membrane permeability had no significant impact on separation performance. A virus loss of 2.1% in the flow through, a yield of 57.4% together with a contamination level of 5.1 pgDNA HAU−1 and 1.2 ngprot HAU−1 were experimentally confirmed for the optimal operating point predicted. The critical process parameters identified and their optimal settings should support the optimization of sulfated Cellulose Membrane adsorbers based purification trains for other influenza virus strains, streamlining cell culture-derived vaccine manufacturing. This article is protected by copyright. All rights reserved
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sulfated Membrane adsorbers for economic pseudo affinity capture of influenza virus particles
Biotechnology and Bioengineering, 2009Co-Authors: L Opitz, Michael W Wolff, Udo Reichl, S LehmannAbstract:Strategies to control outbreaks of influenza, a contagious respiratory tract disease, are focused mainly on prophylactic vaccinations in conjunction with antiviral medications. Currently, several mammalian cell culture-based influenza vaccine production processes are being established, such as the technologies introduced by Novartis Behring (Optaflu®) or Baxter International Inc. (Celvapan). Downstream processing of influenza virus vaccines from cell culture supernatant can be performed by adsorbing virions onto sulfated column chromatography beads, such as Cellufine® sulfate. This study focused on the development of a sulfated Cellulose Membrane (SCM) chromatography unit operation to capture cell culture-derived influenza viruses. The advantages of the novel method were demonstrated for the Madin Darby canine kidney (MDCK) cell-derived influenza virus A/Puerto Rico/8/34 (H1N1). Furthermore, the SCM-adsorbers were compared directly to column-based Cellufine® sulfate and commercially available cation-exchange Membrane adsorbers. Sulfated Cellulose Membrane adsorbers showed high viral product recoveries. In addition, the SCM-capture step resulted in a higher reduction of dsDNA compared to the tested cation-exchange Membrane adsorbers. The productivity of the SCM-based unit operation could be significantly improved by a 30-fold increase in volumetric flow rate during adsorption compared to the bead-based capture method. The higher flow rate even further reduced the level of contaminating dsDNA by about twofold. The reproducibility and general applicability of the developed unit operation were demonstrated for two further MDCK cell-derived influenza virus strains: A/Wisconsin/67/2005 (H3N2) and B/Malaysia/2506/2004. Overall, SCM-adsorbers represent a powerful and economically favorable alternative for influenza virus capture over conventional methods using Cellufine® sulfate. Biotechnol. Bioeng. 2009;103: 1144–1154. © 2009 Wiley Periodicals, Inc.
Michael W Wolff - One of the best experts on this subject based on the ideXlab platform.
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use of sulfated Cellulose Membrane adsorbers for chromatographic purification of cell cultured derived influenza a and b viruses
Separation and Purification Technology, 2019Co-Authors: Raquel A Fortuna, Louis Villain, Sebastian Van Teeffelen, Adrian Ley, Laura M Fischer, Florian Taft, Yvonne Genzel, Michael W Wolff, Udo ReichlAbstract:Abstract Influenza virus particles can be purified by pseudo-affinity chromatography using sulfated carbohydrate matrices. In this study, we compared the binding capacity and the purification performance of two bead-based resins and one Membrane adsorber for three influenza virus strains (H1N1, H3N2 and B) produced in MDCK suspension cells in a chemically defined medium. The dynamic binding capacity for the sulfated Cellulose Membrane adsorbers was consistently higher than for the resins (8 to 22-fold). Confocal microscopy demonstrated, that while virus particles uniformly covered the complete surface of the Membrane, the virus particles only bound to the external surface of the sulfated beads. Overall, recovery of virus varied between 66% and 81%. Total protein and DNA removal were >74% and >96%, respectively. Statistically significant differences in the purification performance were more often observed between strains than between matrices. In particular, H3N2 influenza virus particles could only be purified using the sulfated Membrane adsorbers. Due to the higher operating flow rate and binding capacity, the productivity with the Membrane adsorbers was on average 25-times higher than with the resins.
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purification of influenza virus like particles using sulfated Cellulose Membrane adsorbers
Journal of Chemical Technology & Biotechnology, 2018Co-Authors: Sofia B Carvalho, Raquel A Fortuna, Michael W Wolff, Udo Reichl, Cristina Peixoto, Paula M Alves, Manuel J T CarrondoAbstract:BACKGROUND Vaccines based on virus-like particles (VLPs) are an alternative to inactivated viral vaccines that combine good safety profiles with strong immunogenicity. In order to be economically competitive, efficient manufacturing is required, in particular downstream processing, which often accounts for major production costs. This study describes the optimization and establishment of a chromatography capturing technique using sulfated Cellulose Membrane adsorbers (SCMA) for purification of influenza VLPs. RESULTS Using a design of experiments approach, the critical factors for SCMA performance were described and optimized. For optimal conditions (Membrane ligand density: 15.4 µmol cm-2, salt concentration of the loading buffer: 24 mmol L-1 NaCl, and elution buffer: 920 mmol L-1 NaCl, as well as the corresponding flow rates: 0.24 and 1.4 mL min-1), a yield of 80% in the product fraction was obtained. No loss of VLPs was detected in the flowthrough fraction. Removal of total protein and DNA impurities were higher than 89% and 80%, respectively. CONCLUSION Use of SCMA represents a significant improvement compared with conventional ion exchanger Membrane adsorbers. As the method proposed is easily scalable and reduces the number of steps required compared with conventional purification methods, SCMA could qualify as a generic platform for purification of VLP-based influenza vaccines. © 2017 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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optimization of cell culture derived influenza a virus particles purification using sulfated Cellulose Membrane adsorbers
Engineering in Life Sciences, 2018Co-Authors: Ana Raquel Fortuna, Louis Villain, Florian Taft, Michael W Wolff, Udo ReichlAbstract:Downstream processing remains one of the biggest challenges in manufacturing of biologicals and vaccines. This work focuses on a Design of Experiments approach to understand factors influencing the performance of sulfated Cellulose Membrane adsorbers for the chromatographic purification of a cell culture-derived H1N1 influenza virus strain (A/Puerto Rico/8/34). Membranes with a medium ligand density together with low conductivity and a high virus titer in the feed stream resulted in optimum virus yields and low protein and DNA content in the product fraction. Flow rate and salt concentration in the buffer used for elution were of secondary importance while Membrane permeability had no significant impact on separation performance. A virus loss of 2.1% in the flow through, a yield of 57.4% together with a contamination level of 5.1 pgDNA HAU−1 and 1.2 ngprot HAU−1 were experimentally confirmed for the optimal operating point predicted. The critical process parameters identified and their optimal settings should support the optimization of sulfated Cellulose Membrane adsorbers based purification trains for other influenza virus strains, streamlining cell culture-derived vaccine manufacturing. This article is protected by copyright. All rights reserved
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sulfated Membrane adsorbers for economic pseudo affinity capture of influenza virus particles
Biotechnology and Bioengineering, 2009Co-Authors: L Opitz, Michael W Wolff, Udo Reichl, S LehmannAbstract:Strategies to control outbreaks of influenza, a contagious respiratory tract disease, are focused mainly on prophylactic vaccinations in conjunction with antiviral medications. Currently, several mammalian cell culture-based influenza vaccine production processes are being established, such as the technologies introduced by Novartis Behring (Optaflu®) or Baxter International Inc. (Celvapan). Downstream processing of influenza virus vaccines from cell culture supernatant can be performed by adsorbing virions onto sulfated column chromatography beads, such as Cellufine® sulfate. This study focused on the development of a sulfated Cellulose Membrane (SCM) chromatography unit operation to capture cell culture-derived influenza viruses. The advantages of the novel method were demonstrated for the Madin Darby canine kidney (MDCK) cell-derived influenza virus A/Puerto Rico/8/34 (H1N1). Furthermore, the SCM-adsorbers were compared directly to column-based Cellufine® sulfate and commercially available cation-exchange Membrane adsorbers. Sulfated Cellulose Membrane adsorbers showed high viral product recoveries. In addition, the SCM-capture step resulted in a higher reduction of dsDNA compared to the tested cation-exchange Membrane adsorbers. The productivity of the SCM-based unit operation could be significantly improved by a 30-fold increase in volumetric flow rate during adsorption compared to the bead-based capture method. The higher flow rate even further reduced the level of contaminating dsDNA by about twofold. The reproducibility and general applicability of the developed unit operation were demonstrated for two further MDCK cell-derived influenza virus strains: A/Wisconsin/67/2005 (H3N2) and B/Malaysia/2506/2004. Overall, SCM-adsorbers represent a powerful and economically favorable alternative for influenza virus capture over conventional methods using Cellufine® sulfate. Biotechnol. Bioeng. 2009;103: 1144–1154. © 2009 Wiley Periodicals, Inc.
Jian Shen - One of the best experts on this subject based on the ideXlab platform.
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grafting of carboxybetaine brush onto Cellulose Membranes via surface initiated arget atrp for improving blood compatibility
Colloids and Surfaces B: Biointerfaces, 2013Co-Authors: Miao Wang, Jiang Yuan, Xiaobo Huang, Li Li, Jian ShenAbstract:Grafting-from has proven to be a very effective way to create high grafting densities and well-controlled polymer chains on different kinds of surfaces. In this work, we aim to graft zwitterionic brush from Cellulose Membrane (CM) via ARGET-ATRP (Activator Regenerated by Electron Transfer ATRP) method indirectly for blood compatibility improvement. Characterization of the CM substrates before and after modification was carried out by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), water contact angle measurements, X-ray photoelectron spectroscopy analysis, and atomic force microscopy, respectively. The results demonstrated zwitterionic brushes were successfully grafted on the CM surfaces, and the content of the grafted layer increased gradually with the polymerization time. The platelet adhesion, hemolytic test and plasma protein adsorption results indicated the Cellulose Membrane had significantly excellent blood compatibility featured on lower platelet adhesion and protein adsorption without causing hemolysis. The functionalized Cellulose substrate could have a great potential usage for biomedical applications.
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grafting of zwitterion from Cellulose Membranes via atrp for improving blood compatibility
Biomacromolecules, 2009Co-Authors: Qiang Chen, Shishan Wu, Bo Yuan, Jian ShenAbstract:A p-vinylbenzyl sulfobetaine was grafted from Cellulose Membrane (CM) using surface-initiated atom transfer radical polymerization for blood compatibility improvement. Surface structure, wettability, morphology, and thermal stability of the CM substrates before and after modification were characterized by attenuated total reflectance Fourier transform infrared spectra, X-ray photoelectron spectroscopy measurement, water contact angle measurement, atomic force microscopy, and thermogravimetric analysis, respectively. The results showed that zwitterionic brushes were successfully fabricated on the CM surfaces, and the content of the grafted layer increased gradually with the polymerization time. The blood compatibility of the CM substrates was evaluated by protein adsorption tests and platelet adhesion tests in vitro. It was found that all the CMs functionalized with zwitterionic brush showed improved resistance to nonspecific protein adsorption and platelet adhesion, even though the grafting polymerization...
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grafting of zwitterion from Cellulose Membranes via atrp for improving blood compatibility
Biomacromolecules, 2009Co-Authors: Pingsheng Liu, Jian Shen, Qiang Chen, Bo Yuan, Xiang Liu, Sicong LinAbstract:A p-vinylbenzyl sulfobetaine was grafted from Cellulose Membrane (CM) using surface-initiated atom transfer radical polymerization for blood compatibility improvement. Surface structure, wettability, morphology, and thermal stability of the CM substrates before and after modification were characterized by attenuated total reflectance Fourier transform infrared spectra, X-ray photoelectron spectroscopy measurement, water contact angle measurement, atomic force microscopy, and thermogravimetric analysis, respectively. The results showed that zwitterionic brushes were successfully fabricated on the CM surfaces, and the content of the grafted layer increased gradually with the polymerization time. The blood compatibility of the CM substrates was evaluated by protein adsorption tests and platelet adhesion tests in vitro. It was found that all the CMs functionalized with zwitterionic brush showed improved resistance to nonspecific protein adsorption and platelet adhesion, even though the grafting polymerization was conducted for several minutes.
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various approaches to modify biomaterial surfaces for improving hemocompatibility
Advances in Colloid and Interface Science, 2004Co-Authors: Chun Mao, Jian Shen, Yongzhi Qiu, Haibo Sang, Hua Mei, Aiping Zhu, Sicong LinAbstract:In this paper, the mechanism of thrombus formation on the surface of polymeric materials and the various approaches of modifying biomaterial surfaces to improve their hemocompatibility are reviewed. Moreover, the blood compatibility of the Cellulose Membrane grafted with O-butyrylchitosan (OBCS) by using a radiation grafting technique was studied. Surface analysis of grafted Cellulose Membrane was verified by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) and electron spectroscopy for chemical analysis (ESCA), which confirmed that OBCS was successfully grafted onto the Cellulose Membrane surfaces. Blood compatibility of the grafted Cellulose Membranes was evaluated by platelet rich plasma (PRP) contacting experiments and protein adsorption experiments using blank Cellulose Membranes as the control. The blood compatibility of OBCS grafted Cellulose Membranes is better than that of blank Cellulose Membranes. These results suggest that the photocrosslinkable chitosan developed here has the potential of serving in blood-contacting applications in medical use.
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chemical modification of Cellulose Membranes with sulfo ammonium zwitterionic vinyl monomer to improve hemocompatibility
Colloids and Surfaces B: Biointerfaces, 2003Co-Authors: Jun Zhang, Jiang Yuan, Jian Shen, Youling Yuan, Sicong LinAbstract:Platelets from human plasma adhered on the Cellulose Membranes grafted with N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl) ammonium (DMMSA) was studied. DMMSA was directly graft-polymerized onto the Cellulose Membranes using ceric ammonium nitrate (CAN) as initiator. Surface analysis of the grafted Membranes by ATR-FTIR and ESCA confirmed that DMMSA was successfully grafted onto the SPEU Membrane surface. The amount of PDMMSA grafted onto Cellulose Membranes increased with increasing the feed monomer concentration. The platelet resistant property of the grafted Membranes was evaluated by a platelet-rich plasma adhesion study and scanning electron microscopy, using original Cellulose Membranes as the control. The results showed that platelet-adhesive resistance of the modified Cellulose Membrane has been greatly improved. This kind of new biomaterials grafted with sulfo ammonium zwitterionic vinyl monomers might have potential for biomedical applications.
Surachai Saejung - One of the best experts on this subject based on the ideXlab platform.
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chitosan pad Cellulose Membrane or gelatin sponge for peridural bleeding an efficacy study on a lumbar laminectomized rat model
Asian Spine Journal, 2018Co-Authors: Surachai Saejung, Punyawat ApiwatanakulAbstract:Study Design Experimental study in an animal model. Purpose This study aims to evaluate the hemostatic properties of four common hemostatic materials including the chitosan clot pad, absorbable gelatin sponge, Cellulose Membrane, and gauze on peridural bleeding using a rat model. Overview of Literature Intraoperative bleeding during spinal surgery can lead to morbidities. Hemostatic materials have been developed, but the efficacy of these materials on peridural bleeding remains unclear. Methods Forty 8-week-old Sprague-Dawley rats were used in this study. Under adequate anesthesia, each rat was posteriorly dissected to their L5 and L6 spinous processes. Bleeding from muscles and soft tissue dissections was stopped before lumbar bone cutting. Immediately after the L5-L6 laminae were cut and removed, the rats were randomly allocated to receive one of the abovementioned hemostatic materials. All hemostatic materials were placed over the raw surface of cut bone and dura and changed every 60 seconds. The procedure was stopped when there was no further bleeding. Time to staunching and amount of bleeding were recorded for statistical analysis. Results The respective mean±standard deviation hemorrhage volume and time for the Cellulose Membrane, gelatin sponge, chitosan pad, and gauze were 1.19±0.44, 1.03±0.72, 0.96±0.57, and 1.98±0.62 mL, respectively, and 2.9±0.6, 2.1±0.6, 1.7±0.5, and 2.9±1.0 minutes, respectively. The overall bleeding volumes for the Cellulose Membrane, gelatin sponge, and chitosan pad were significantly lower than the overall bleeding volume for gauze. Conclusions Chitosan clot pads, gelatin sponges, and Cellulose Membranes have better hemostatic properties than gauze. The chitosan pad had the lowest average bleeding volume, followed by gelatin sponge and Cellulose Membrane.
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prevention of peridural fibrosis using a cyclooxygenase 2 inhibitor nonsteroidal anti inflammatory drug soaked in absorbable gelatin sponge an experimental comparative animal model
Spine, 2013Co-Authors: Surachai Saejung, Kitti JirarattanaphochaiAbstract:Abstract Experimental study. To evaluate the efficacy and safety of peridural parecoxib-soaked absorbable gelatin sponge, and Cellulose Membrane on peridural fibrosis prevention in an animal model. Postoperative peridural fibrosis is one of the causes of failed back surgery syndrome. Nonsteroidal anti-inflammatory drugs inhibit the inflammatory response, while an absorbable gelatin sponge or Cellulose Membrane interposes between the dura and the paraspinal muscle to staunch the surgical bleeding. These mechanisms may prevent peridural fibrosis. Forty L5-L6 laminectomized adult Sprague-Dawley rats were randomly allocated into 4 groups. The high parecoxib group received 6 mg of parecoxib soaked into an absorbable gelatin sponge placed over the dura. The low parecoxib group was given 2 mg of parecoxib soaked into an absorbable gelatin sponge. The dura in the Cellulose group was covered with a Cellulose Membrane, while the control group received normal saline drip before surgical wound closure. All rats were killed at 6 weeks for histopathological assessment. The fibroblast density, inflammatory cell density, fibrous adherence, and adverse events were quantified. The obtained results were analyzed statistically. The respective mean fibroblast density in the high parecoxib, low parecoxib, Cellulose, and control groups was 217.77 ± 51.76, 317.51 ± 126.92, 321.80 ± 90.94, and 328.48 ± 73.41 cells/mm², while the respective mean inflammatory cell density was 539.65 ± 236.52, 910.17 ± 242.59, 1011.84 ± 239.30, and 1261.78 ± 319.68 cells/mm². The mean fibroblast and inflammatory cell densities of the high parecoxib group were significantly lower than the control. The high parecoxib group also showed statistically less fibrous adherence than low parecoxib, Cellulose, and control groups. The high-dose parecoxib-soaked absorbable gelatin sponge can prevent peridural fibrosis without complications. The low-dose parecoxib and Cellulose Membrane provided no significant benefit vis-a-vis prevention of peridural fibrosis, as adduced from the lack of any statistically significant difference between the test and control rats.
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prevention of peridural fibrosis using a cyclooxygenase 2 inhibitor nonsteroidal anti inflammatory drug soaked in absorbable gelatin sponge an experimental comparative animal model
Spine, 2013Co-Authors: Surachai Saejung, Kitti JirarattanaphochaiAbstract:Study design Experimental study. Objective To evaluate the efficacy and safety of peridural parecoxib-soaked absorbable gelatin sponge, and Cellulose Membrane on peridural fibrosis prevention in an animal model. Summary of background data Postoperative peridural fibrosis is one of the causes of failed back surgery syndrome. Nonsteroidal anti-inflammatory drugs inhibit the inflammatory response, while an absorbable gelatin sponge or Cellulose Membrane interposes between the dura and the paraspinal muscle to staunch the surgical bleeding. These mechanisms may prevent peridural fibrosis. Methods Forty L5-L6 laminectomized adult Sprague-Dawley rats were randomly allocated into 4 groups. The high parecoxib group received 6 mg of parecoxib soaked into an absorbable gelatin sponge placed over the dura. The low parecoxib group was given 2 mg of parecoxib soaked into an absorbable gelatin sponge. The dura in the Cellulose group was covered with a Cellulose Membrane, while the control group received normal saline drip before surgical wound closure. All rats were killed at 6 weeks for histopathological assessment. The fibroblast density, inflammatory cell density, fibrous adherence, and adverse events were quantified. The obtained results were analyzed statistically. Results The respective mean fibroblast density in the high parecoxib, low parecoxib, Cellulose, and control groups was 217.77 ± 51.76, 317.51 ± 126.92, 321.80 ± 90.94, and 328.48 ± 73.41 cells/mm², while the respective mean inflammatory cell density was 539.65 ± 236.52, 910.17 ± 242.59, 1011.84 ± 239.30, and 1261.78 ± 319.68 cells/mm². The mean fibroblast and inflammatory cell densities of the high parecoxib group were significantly lower than the control. The high parecoxib group also showed statistically less fibrous adherence than low parecoxib, Cellulose, and control groups. Conclusion The high-dose parecoxib-soaked absorbable gelatin sponge can prevent peridural fibrosis without complications. The low-dose parecoxib and Cellulose Membrane provided no significant benefit vis-a-vis prevention of peridural fibrosis, as adduced from the lack of any statistically significant difference between the test and control rats.